LOCUS       NZ_RDBM01000070         6541 bp    DNA     linear   CON 01-JAN-2025
DEFINITION  Streptomyces sp. gb1(2016) Scaffold_71, whole genome shotgun
            sequence.
ACCESSION   NZ_RDBM01000070
VERSION     NZ_RDBM01000070.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. gb1(2016)
  ORGANISM  Streptomyces sp. gb1(2016)
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Hariharan,J., Choudoir,M.J., Diebold,P., Panke-Buisse,K.,
            Campbell,A.N. and Buckley,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2018) Soil and Crop Sciences, Cornell University,
            306 Tower Road, Ithaca, NY 14850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: A5+MeDuSa v. JUNE-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008042065.1-RS_2025_01_01
            Annotation Date                   :: 01/01/2025 02:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,414
            CDSs (total)                      :: 7,333
            Genes (coding)                    :: 6,887
            CDSs (with protein)               :: 6,887
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 447
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 447
            Pseudo Genes (frameshifted)       :: 94 of 447
            Pseudo Genes (incomplete)         :: 395 of 447
            Pseudo Genes (internal stop)      :: 7 of 447
            Pseudo Genes (multiple problems)  :: 48 of 447
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:17:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6541
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RDBM01000070.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..2339
                     /locus_tag="EAO74_RS36935"
                     /old_locus_tag="EAO74_36995"
     source          1..6541
                     /collection_date="2009"
                     /db_xref="taxon:1828321"
                     /geo_loc_name="USA: Greensboro, North Carolina"
                     /isolation_source="soil"
                     /lat_lon="36.09 N 79.89 W"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. gb1(2016)"
                     /strain="gb1(2016)"
                     /submitter_seqid="Scaffold_71"
     region          1..6541
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /product="hglE-KS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /rules="(hglE or hglD)"
                     /tool="antismash"
     cand_cluster    1..6541
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /detection_rules="(hglE or hglD)"
                     /kind="chemical_hybrid"
                     /product="T1PKS"
                     /product="hglE-KS"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..6541
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[2383:>6541](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2384..>6541
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     protocluster    1..6541
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[2383:>6541](+)"
                     /cutoff="20000"
                     /detection_rule="(hglE or hglD)"
                     /neighbourhood="20000"
                     /product="hglE-KS"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      2384..>6541
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(hglE or hglD)"
                     /neighbourhood="20000"
                     /product="hglE-KS"
                     /protocluster_number="2"
     CDS             <5..2339
                     /NRPS_PKS="Domain: PKS_KR (238-458). E-value: 1.4e-46.
                     Score: 150.7. Matches aSDomain:
                     nrpspksdomains_EAO74_RS36935_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_DH (513-649). E-value: 2.5e-08.
                     Score: 26.4. Matches aSDomain:
                     nrpspksdomains_EAO74_RS36935_PKS_DH.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019324558.1"
                     /locus_tag="EAO74_RS36935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_36995"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_147986041.1"
                     /sec_met_domain="adh_short (E-value: 4.1e-34, bitscore:
                     105.3, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RRRARPVAVPEADHAIPAPRHEPVRTAEPATELTRLAVRTVETPA
                     PGLTLAGLGDEPIAVIEDGEGVAERVAAGLTGHGLRATVVSSVPPEARAVVHLGGLAPA
                     GSPEAALAIQRDVFDAARSLAPHFTTAGGVFVAVQDTGGDFGLRGSSPERAWLGGIAAL
                     ARTAAREWPTAVVKVIDCERGGRDADALAEAVVRELLDGGPAAEVGLRADGTRTTPVTV
                     PAPVEPGTAARIGTDSVIVATGGARGVTAAALLDLARTHRPRIALLGRTEPAEEPPGLA
                     AATDEAALTRALAERTGGATPAAIGAEARRILAAREIRSTVAALESAGSGVRYVAVDVR
                     DGDALHHALEEVRRDWGPVTGIVHGAGVLADKLIAEKTDEQFDHVLATKAGGLRTLLAA
                     TGNDPLDTIVLFSSVAAVHGNAGQSDYAMANEVLNHVACAERARRPDCLVRSVSWGPWH
                     AGMVTPALAAHFGRAGVPLIPTAQGAAAFTAELGAPGADTRVVLVAAGDGPGPAEAVTD
                     PAAAQVRIDARTHPYLADHDIAGVPVLPVAVALDRFAGAAAAWRPDAGHLVLRDLRVFS
                     KVALPHLDGAGHRLTLSGRRAATGSPSAVEAELAGEEGTPHFRVQLDFEADPPRPESWD
                     TPRGLTAPKDTGIYDGRVLFHGPRFHALNSVHGVGAEGAEATVAGLTELGWGGDHWHLD
                     AAATDGALQLALLWADGALGDATLPMAVAECRVHRRGPVQGPVRCVVRARKVHDTGARC
                     DAALLGPDGSPVVELLGVELVRRPS"
     aSDomain        720..1379
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N found: True"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS36935_PKS_KR.1"
                     /evalue="1.40E-46"
                     /label="EAO74_RS36935_PKS_KR.1"
                     /locus_tag="EAO74_RS36935"
                     /protein_end="458"
                     /protein_start="238"
                     /score="150.7"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="VIVATGGARGVTAAALLDLARTHRPRIALLGRTEPAEEPPGLAAA
                     TDEAALTRALAERTGGATPAAIGAEARRILAAREIRSTVAALESAGSGVRYVAVDVRDG
                     DALHHALEEVRRDWGPVTGIVHGAGVLADKLIAEKTDEQFDHVLATKAGGLRTLLAATG
                     NDPLDTIVLFSSVAAVHGNAGQSDYAMANEVLNHVACAERARRPDCLVRSVSWGPWH"
     aSModule        720..1952
                     /domains="nrpspksdomains_EAO74_RS36935_PKS_KR.1"
                     /domains="nrpspksdomains_EAO74_RS36935_PKS_DH.1"
                     /incomplete
                     /locus_tags="EAO74_RS36935"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       732..818
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EAO74_RS36935_0001"
                     /evalue="1.50E-02"
                     /label="PKSI-KR_m1"
                     /locus_tag="EAO74_RS36935"
                     /protein_end="271"
                     /protein_start="242"
                     /score="8.0"
                     /tool="antismash"
                     /translation="TGGARGVTAAALLDLARTHRPRIALLGRT"
     CDS_motif       1218..1316
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EAO74_RS36935_0002"
                     /evalue="2.00E-13"
                     /label="PKSI-KR_m4"
                     /locus_tag="EAO74_RS36935"
                     /protein_end="437"
                     /protein_start="404"
                     /score="42.9"
                     /tool="antismash"
                     /translation="LDTIVLFSSVAAVHGNAGQSDYAMANEVLNHVA"
     aSDomain        1545..1952
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS36935_PKS_DH.1"
                     /evalue="2.50E-08"
                     /label="EAO74_RS36935_PKS_DH.1"
                     /locus_tag="EAO74_RS36935"
                     /protein_end="649"
                     /protein_start="513"
                     /score="26.4"
                     /tool="antismash"
                     /translation="AVTDPAAAQVRIDARTHPYLADHDIAGVPVLPVAVALDRFAGAAA
                     AWRPDAGHLVLRDLRVFSKVALPHLDGAGHRLTLSGRRAATGSPSAVEAELAGEEGTPH
                     FRVQLDFEADPPRPESWDTPRGLTAPKDTGIY"
     CDS_motif       1599..1661
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EAO74_RS36935_0003"
                     /evalue="1.20E-03"
                     /label="PKSI-DH_m2"
                     /locus_tag="EAO74_RS36935"
                     /protein_end="552"
                     /protein_start="531"
                     /score="11.6"
                     /tool="antismash"
                     /translation="YLADHDIAGVPVLPVAVALDR"
     misc_feature    complement(2365..2379)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.19"
                     /tool="antismash"
     gene            2384..>6541
                     /locus_tag="EAO74_RS36940"
                     /old_locus_tag="EAO74_37000"
     CDS             2384..>6541
                     /NRPS_PKS="Domain: PKS_KS(Trans-AT-KS) (5-433). E-value:
                     3.1e-90. Score: 295.0. Matches aSDomain:
                     nrpspksdomains_EAO74_RS36940_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (934-1158). E-value: 1.7e-23.
                     Score: 75.3. Matches aSDomain:
                     nrpspksdomains_EAO74_RS36940_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hglE-KS: hglD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026868.1"
                     /locus_tag="EAO74_RS36940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_37000"
                     /product="type I polyketide synthase"
                     /protein_id="WP_147986042.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.3e-47, bitscore:
                     148.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="hglD (E-value: 2.5e-155, bitscore: 504.1,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_KS (E-value: 5.4e-18, bitscore: 52.1,
                     seeds: 2284, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 5.8e-25, bitscore: 75.3,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFDPIAVVGRGCVFPDASDPDAFWDNIAARRCSLSPVPDGRWRL
                     PHRWAMGTVDDHLDRTWNDVGGYVRDFGREFDPTGFLMAPDDILGLDPLFHWVLHGTRQ
                     ALREAGREGPSPRTGLVLGNLSYPTSAGSAFAEHVWLSQGAPSLLPAAVRTRPDARNRF
                     SSGLPAHFAARALGMGAGAFALDAACASSLYAVKLACDRLHDGTADLMVAGAVNRADSL
                     FLHVGFCGLSAVSRTSRSRPFHRDADGLLPGEGAGFVALMRLRDALAQGAPVLGVIRGV
                     GLSNDGRGRGLLSPAGEGQERAMRLAYAMAGVAPQSVSLVECHATGTPVGDAEEVRSMA
                     RVFAESPDVPIGSVKSNVGHLLASAGAGGLLKVLGAMRAGLRPATLAAEEPLSELHGTP
                     LRVLGESEEWPGPRRAAVSAFGFGGTNAHLIVDAWDGTGRTRGAPSPPPPRTPRSVEGP
                     LTIVAMAVRAGEGAGTHAFHRAVLEGRPSGPAAEIEVALPGLCFPPLAVGQALPQQILM
                     LEAAREAARNTPLPRDRTLVLVGTGVDTENSRASARWRAPSWLEGAGKPTGPEPAARFR
                     DAFCAPMGAERVVGTLPNLVANRINTQLDLGGAGYTVSAEEASGPVALRLAARALRAGE
                     ADAALVGAVDLSCEPVHRAALDALGRRAVSGDAAVAVVLKRLADARRDGDPVLALLDDE
                     ATGDPGAADVVIGDGAGAGFDPAALFGRAHAAHGLLAVATAVTALRHRALPRTGGPAEA
                     APGLRTADVVVTPLEAPPLRLRLRAADAGAWVPGPWPRGSAPRLYVYSGRDRHEVLAAL
                     ESDTESREGPARLAFVSDGDPRTHRDAVRRWLLGDGARPPGAVYRAGPVAGGTAFVFTN
                     GSAAYPGMGRGVQLALPALGDAVATTYGSAAGWPGPGGAPTVLDRIWGAARLAAVHTEL
                     TRGLLGLRPDAALGYSSGESAALAALGAWRDVAALHRDTWESGLFTRELTGELRAVRRA
                     WERLGVEGDRWSSYLVTAPLDQVRAELADERAVHLMAVNAPGICVVGGESQACAAVVER
                     LGTAHAIPVDYDLAAHAPELSEVRDLWWKLHHRRTHAVPGVRFYSGATTLPYAPTAERA
                     ADAVTAQGMGTIDFVGTVERAWADGVRVFVEHGPRGLCTGWIKRILGDREHVAVALDAE
                     DGGLRPLYLAVAELVAAGVPVRTEALFAHLRTAAPAPADDGPTLTVPAHWEITLPSLEE
                     PAVIMPRAPRLSPVPAHRPTEPAESAAAVSVPAAVSVPAPAPAPAPVSPSAPVPAPLSA
                     PAPVPAGTEPRAATGTEPRPPDLRTLVAQHTGRVIALHQDVLAQHLAVHQRFLRTRAQL
                     ATALVHVAAGTGWDGATLPAATCTSAVASWALVLRNRWCTARC"
     aSDomain        2399..3682
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: False"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS36940_PKS_KS.1"
                     /domain_subtypes="Trans-AT-KS"
                     /evalue="3.10E-90"
                     /label="EAO74_RS36940_PKS_KS.1"
                     /locus_tag="EAO74_RS36940"
                     /protein_end="433"
                     /protein_start="5"
                     /score="295.0"
                     /specificity="transATor: (unknown)"
                     /tool="antismash"
                     /translation="IAVVGRGCVFPDASDPDAFWDNIAARRCSLSPVPDGRWRLPHRWA
                     MGTVDDHLDRTWNDVGGYVRDFGREFDPTGFLMAPDDILGLDPLFHWVLHGTRQALREA
                     GREGPSPRTGLVLGNLSYPTSAGSAFAEHVWLSQGAPSLLPAAVRTRPDARNRFSSGLP
                     AHFAARALGMGAGAFALDAACASSLYAVKLACDRLHDGTADLMVAGAVNRADSLFLHVG
                     FCGLSAVSRTSRSRPFHRDADGLLPGEGAGFVALMRLRDALAQGAPVLGVIRGVGLSND
                     GRGRGLLSPAGEGQERAMRLAYAMAGVAPQSVSLVECHATGTPVGDAEEVRSMARVFAE
                     SPDVPIGSVKSNVGHLLASAGAGGLLKVLGAMRAGLRPATLAAEEPLSELHGTPLRVLG
                     ESEEWPGPRRAAVSAFGFGGTNAHLIVDA"
     aSModule        2399..5857
                     /domains="nrpspksdomains_EAO74_RS36940_PKS_KS.1"
                     /domains="nrpspksdomains_EAO74_RS36940_PKS_AT.1"
                     /incomplete
                     /locus_tags="EAO74_RS36940"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       3344..3388
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EAO74_RS36940_0001"
                     /evalue="4.30E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="EAO74_RS36940"
                     /protein_end="335"
                     /protein_start="320"
                     /score="9.8"
                     /tool="antismash"
                     /translation="VECHATGTPVGDAEE"
     aSDomain        5186..5857
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS36940_PKS_AT.1"
                     /evalue="1.70E-23"
                     /label="EAO74_RS36940_PKS_AT.1"
                     /locus_tag="EAO74_RS36940"
                     /protein_end="1158"
                     /protein_start="934"
                     /score="75.3"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: (unknown)"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="LGLRPDAALGYSSGESAALAALGAWRDVAALHRDTWESGLFTREL
                     TGELRAVRRAWERLGVEGDRWSSYLVTAPLDQVRAELADERAVHLMAVNAPGICVVGGE
                     SQACAAVVERLGTAHAIPVDYDLAAHAPELSEVRDLWWKLHHRRTHAVPGVRFYSGATT
                     LPYAPTAERAADAVTAQGMGTIDFVGTVERAWADGVRVFVEHGPRGLCTGWIKRILGDR
                     EH"
ORIGIN
        1 cccgtcgccg tgcccgaccc gtcgccgtgc ccgaggcgga ccacgcgatc cccgctcccc
       61 ggcatgagcc ggtgcgcacg gccgagccgg ccaccgagct gaccaggctc gccgtccgta
      121 cggtcgagac ccccgcaccc gggctcaccc tggccggtct cggggacgag ccgatcgccg
      181 tcatcgagga cggcgagggc gtcgcggagc gggtggccgc cggactgacc ggccacggcc
      241 tgcgcgccac cgtcgtctcc agcgtgcccc cggaggcgcg tgcggtcgtg cacctcgggg
      301 gcctcgcccc ggccggatcg cccgaggccg cgctggcgat ccagcgggac gtcttcgacg
      361 cggccaggtc gctcgcgccg cacttcacca ccgcaggcgg ggtgttcgtc gccgtacagg
      421 acaccggcgg cgacttcggg ctgcgcggca gcagcccgga gcgggcctgg ctcggcggga
      481 tcgccgcgct cgccaggacc gccgccaggg aatggcccac ggccgtggtc aaggtgatcg
      541 actgcgagcg cggcgggcgt gacgccgacg ccctcgccga agcggtcgta cgggaactgc
      601 tcgacggcgg acccgcggcg gaggtcggcc tccgcgcgga cggcacccgc accacccccg
      661 tcacggtccc cgcgccggtg gagccgggga cggccgcgcg catcggtacg gactccgtca
      721 tcgtggccac cggcggggcc cggggagtga cggcggccgc gctgctggac ctcgccagga
      781 cgcaccggcc gcgcatcgcg ctgctcggcc gcaccgagcc cgccgaggaa ccgccggggc
      841 tggcggcggc gaccgacgag gccgcgctca cccgggccct cgcggaacgg accggcgggg
      901 ccacccccgc cgcgatcggg gccgaggccc ggaggatcct ggccgcccgc gagatccggt
      961 ccacggtggc ggcgctggag agcgccggat ccggcgtccg gtacgtggcg gtggacgtcc
     1021 gggacggcga cgcactccac cacgcgctgg aagaggtgcg ccgtgactgg gggccggtca
     1081 ccgggatcgt gcacggcgcc ggggtgctgg cggacaagct gatcgccgag aagaccgacg
     1141 agcagttcga ccacgtcctg gccaccaagg cgggcggtct gcgaaccctg ctcgccgcga
     1201 ccgggaacga cccgctcgac accatcgtcc tcttctcctc cgtggccgcc gttcacggca
     1261 acgccggaca gagcgactac gccatggcca acgaggtgct caaccacgtc gcctgcgccg
     1321 agcgcgccag aaggcccgac tgcctggtgc ggtcggtctc ctggggcccg tggcacgcag
     1381 ggatggtcac acccgcactg gccgcgcact tcggccgggc cggggtgccc ctcatcccca
     1441 ccgcacaggg cgccgccgcc ttcaccgccg aactgggcgc tccgggcgcc gacaccaggg
     1501 tggtgctcgt cgcggccggg gacggccccg gcccggccga agccgtcacc gatccggccg
     1561 cggcgcaggt gcgcatcgac gcgcggacgc atccctacct cgccgaccac gacatcgccg
     1621 gcgtccccgt cctgccggtg gccgtcgccc tggaccggtt cgcgggcgcc gccgcggcct
     1681 ggcggccgga cgccggccac ctggtgctgc gggacctccg cgtcttcagc aaggtcgccc
     1741 ttccgcacct cgacggcgcg ggccaccggc tcaccctgag cggccgccgc gcggcgaccg
     1801 gatccccctc ggccgtggag gccgaactcg cgggggagga gggcacaccg cacttccggg
     1861 tccagctgga cttcgaggcg gacccgccgc gcccggagag ctgggacacc ccccgagggc
     1921 tcaccgcccc gaaggacacc ggcatctacg acggcagggt cctgttccac ggcccccggt
     1981 tccacgccct gaactccgtc cacggcgtcg gtgcggaggg cgccgaggcg acggtggccg
     2041 gcctgaccga actcggctgg ggcggtgacc actggcatct cgacgcggcc gccacggacg
     2101 gtgccctgca actcgcactg ctgtgggcgg acggcgcgct cggtgacgcc accctgccga
     2161 tggcggtcgc ggagtgccgt gtgcaccggc gcggccccgt ccagggcccg gtgcgctgcg
     2221 tggtgcgggc caggaaggtg cacgacacgg gggcgcgctg cgacgccgcc ctcctcggcc
     2281 cggacggctc ccctgtggtg gagctgctcg gcgtggagct cgtgcggcgc ccctcctgac
     2341 cccgcatcac ccggatcgtc cgtgttcgtg aaatgagacc ggtatggcat tcgacccgat
     2401 cgccgtcgtc ggcagggggt gcgtcttccc cgacgcgtcc gaccccgacg ccttctggga
     2461 caacatcgcc gcgcgacggt gcagtctgtc gccggtcccg gacggcaggt ggcggctgcc
     2521 gcaccggtgg gccatgggca ccgtggacga ccacctcgac cgtacgtgga acgatgtcgg
     2581 cggttacgta cgggacttcg ggcgggagtt cgacccgacc gggttcctca tggcccccga
     2641 cgacatcctc ggcctggacc cgctgttcca ctgggtgctc cacggcaccc ggcaggcgct
     2701 gcgggaagcg ggacgcgagg ggccttcgcc ccgcaccggg ctggtgctgg gcaacctctc
     2761 ctacccgacg agcgcgggct cggccttcgc cgagcacgta tggctgtcgc agggggcgcc
     2821 ctccctgctg cccgcggccg tacggaccag gcccgacgcc cgtaaccgtt tctcctcggg
     2881 gctccccgcg cacttcgcgg ccagggccct ggggatgggc gcgggggcct tcgcgctcga
     2941 cgccgcctgc gcctcctccc tgtacgccgt caaactggcc tgcgaccgcc tccacgacgg
     3001 gaccgcggac ctcatggtcg ccggtgccgt caaccgggcc gacagcctgt tcctgcacgt
     3061 ggggttctgc ggcctgtccg ccgtgagccg caccagccgc agccgcccct tccaccggga
     3121 cgccgacggg ctgctccccg gtgagggcgc cgggttcgtc gcgctgatgc gcctgcggga
     3181 cgccctcgcg cagggtgcgc cggtcctggg agtcatccgg ggagtcgggc tgtccaacga
     3241 cggacgcggc agggggctgc tcagccccgc gggggagggg caggagcggg cgatgcgtct
     3301 ggcgtacgcc atggccgggg tcgcgccgca gtccgtctcg ctcgtggagt gccacgcgac
     3361 gggcacaccc gtcggggacg cggaagaggt ccgcagcatg gcgcgggtct tcgcggagag
     3421 ccctgatgtg cccatcggct cggtcaagtc caacgtcggg cacctgctgg cctcggccgg
     3481 tgcgggcggg ctgctcaagg tgctcggagc gatgcgggcg ggcctccgcc ccgccaccct
     3541 ggccgccgag gaaccgctgt ccgagctgca cggcacaccg ctgcgcgtcc tgggtgagtc
     3601 cgaggagtgg cccggtccac gccgggcggc cgtcagcgcc ttcgggttcg gaggcaccaa
     3661 cgcccacctg atcgtcgacg cctgggacgg cacgggacgt acgaggggtg ccccgtcacc
     3721 gccgccgccc cgtacaccac gctcggtgga gggaccgctg acgatcgtgg cgatggccgt
     3781 ccgggccggc gagggggcgg gcacgcacgc cttccaccgc gcggtgctcg aaggccgccc
     3841 gagcggtccg gcggcggaga tcgaggtggc cctgccgggc ctctgcttcc cgcccctggc
     3901 ggtcggacag gccctgcccc agcagatcct gatgctcgaa gcagcacggg aggcggcacg
     3961 gaacaccccc ctgccgcggg accggaccct ggtcctcgtc ggcaccggcg tggacacgga
     4021 gaacagccgt gcctccgcgc gctggcgggc cccgtcctgg ctggagggcg ccgggaagcc
     4081 gaccggaccg gaaccggccg cgcggttccg ggacgccttc tgcgcgccca tgggcgcgga
     4141 gcgagtggtc ggcaccctgc cgaacctcgt ggccaaccgg atcaacaccc aactcgacct
     4201 gggcggcgcc ggatacaccg tgtcggccga ggaggcgtcc ggcccggtgg cgctgcgtct
     4261 cgcggcacgc gcgctccggg ccggggaggc ggacgcggcc ctcgtcggcg ccgtcgacct
     4321 ctcctgcgaa ccggtccacc gggccgctct ggacgcgctc ggacggcggg ccgtcagcgg
     4381 tgacgcggcc gtggcggtcg tcctcaaacg tctcgccgac gcccgccggg acggcgaccc
     4441 cgtactggcg ctcctcgacg acgaggccac cggtgacccg ggcgcggcgg acgtggtcat
     4501 cggtgacggc gccggcgccg ggttcgaccc ggccgcgctc ttcggccgtg cccacgccgc
     4561 ccatggtctg ctggccgtcg ccaccgccgt gaccgccctg cggcaccgcg ccctgccgcg
     4621 gacgggaggc cccgccgagg ccgccccggg gctgcgtacc gcggacgtgg tggtgacacc
     4681 gctggaggcg cccccgctgc ggctgcggct acgggccgcc gacgccgggg cctgggtgcc
     4741 cggcccctgg ccgcgcggct ccgcaccccg gctgtacgtg tactccgggc gcgaccgcca
     4801 cgaggtcctg gccgcgctgg agtcggacac cgagtcccgc gaagggcccg ccaggctggc
     4861 gttcgtgtcc gacggcgacc ctcggaccca tcgggacgcc gtacgccgct ggctcctcgg
     4921 cgacggtgcc aggccgcccg gcgcggtgta ccgcgcgggg ccggtggcgg gcgggaccgc
     4981 cttcgtgttc accaacgggt cggccgccta cccgggcatg ggccgcgggg tgcagctggc
     5041 cctgcccgcg ctcggcgacg cggtcgcgac cacgtacggg agcgccgccg ggtggccagg
     5101 acccggtggc gcgccgacgg tgctcgaccg gatctggggc gctgcccgac tggccgccgt
     5161 ccacacggaa ctgacccggg gtctgctcgg actgcgaccc gacgccgccc tcggctactc
     5221 gtccggcgag tccgccgccc tggccgccct gggggcctgg cgggacgtgg ccgctctcca
     5281 ccgggacacc tgggagagcg gcctgttcac ccgcgaactc acgggcgagc tgcgggcggt
     5341 acgccgcgcc tgggaacggc tcggcgtgga aggggaccgg tggtcgagct acctggtcac
     5401 agcgcccctc gaccaggtgc gtgcggaact ggcggacgaa cgcgccgtcc atctgatggc
     5461 cgtcaacgcc cccggcatct gcgtggtcgg cggcgagtca caggcgtgcg cggccgtcgt
     5521 ggagcggctc ggcaccgccc acgcgatccc ggtcgactac gacctcgccg cccacgcccc
     5581 cgagctgtcc gaggtccgtg acctgtggtg gaagctgcat caccgccgca cccacgccgt
     5641 gccgggggtg cggttctaca gcggggcgac caccctcccg tacgccccca ccgccgagcg
     5701 tgcggccgac gccgtcaccg cgcagggcat ggggacgatc gacttcgtgg gcaccgtcga
     5761 acgggcatgg gccgacggcg tccgggtgtt cgtcgagcac gggccacgcg gcctgtgcac
     5821 cggatggatc aagcggatcc tgggcgaccg cgagcatgtg gccgtcgcgc tcgacgccga
     5881 ggacgggggg ctgcgcccgc tgtacctggc cgtggcggaa ctcgtcgccg ccggcgtgcc
     5941 cgtccgtacg gaggccctct tcgcgcacct gcgaaccgcc gcgcccgcac cggccgatga
     6001 cggccccacc ctcaccgtcc ccgcccactg ggagatcacc ttgccgtccc tggaagaacc
     6061 cgcggtgatc atgccgaggg cgccccggct gtccccggtc ccggcccacc ggccgaccga
     6121 gcccgcggag tccgccgcgg ccgtttccgt acccgcggcc gtttccgtac ctgcgcctgc
     6181 gccagcgccc gcgcccgttt ccccatccgc gcccgtaccc gcgcctctct ccgcccccgc
     6241 gccggtcccc gccggtacgg aaccgcgggc cgccaccggt acggaaccgc ggcccccgga
     6301 cctgcgtacg ctcgtcgcgc agcacaccgg ccgcgtgatc gcgctgcacc aggacgtcct
     6361 cgcgcaacac ctggccgtgc accagcggtt cctgaggacc agggcccagc tcgccaccgc
     6421 gctcgtacac gtggcggcgg ggacgggctg ggacggggcc acgcttcccg ccgccacgtg
     6481 tacgagcgcg gtggcgagct gggccctggt cctcaggaac cgctggtgca cggccaggtg
     6541 t
//
