LOCUS       NZ_RDBM01000066         1512 bp    DNA     linear   CON 01-JAN-2025
DEFINITION  Streptomyces sp. gb1(2016) Scaffold_67, whole genome shotgun
            sequence.
ACCESSION   NZ_RDBM01000066
VERSION     NZ_RDBM01000066.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. gb1(2016)
  ORGANISM  Streptomyces sp. gb1(2016)
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Hariharan,J., Choudoir,M.J., Diebold,P., Panke-Buisse,K.,
            Campbell,A.N. and Buckley,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2018) Soil and Crop Sciences, Cornell University,
            306 Tower Road, Ithaca, NY 14850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: A5+MeDuSa v. JUNE-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008042065.1-RS_2025_01_01
            Annotation Date                   :: 01/01/2025 02:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,414
            CDSs (total)                      :: 7,333
            Genes (coding)                    :: 6,887
            CDSs (with protein)               :: 6,887
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 447
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 447
            Pseudo Genes (frameshifted)       :: 94 of 447
            Pseudo Genes (incomplete)         :: 395 of 447
            Pseudo Genes (internal stop)      :: 7 of 447
            Pseudo Genes (multiple problems)  :: 48 of 447
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:17:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1512
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RDBM01000066.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..628
                     /locus_tag="EAO74_RS36830"
                     /old_locus_tag="EAO74_36890"
     source          1..1512
                     /collection_date="2009"
                     /db_xref="taxon:1828321"
                     /geo_loc_name="USA: Greensboro, North Carolina"
                     /isolation_source="soil"
                     /lat_lon="36.09 N 79.89 W"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. gb1(2016)"
                     /strain="gb1(2016)"
                     /submitter_seqid="Scaffold_67"
     region          1..1512
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..1512
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1512
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[<940:1438](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<941..1438)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     CDS             <3..628
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010071295.1"
                     /locus_tag="EAO74_RS36830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_36890"
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="WP_187282035.1"
                     /transl_table=11
                     /translation="GTGRRISRSSSSPERIRPTHEARNSVNFTRWSARLPGTQRRAAAP
                     TPTPTPAPPAPEEPPPAPEVYQVNQLAYSLTGDHTGPEILLGRSQGVFWQRQGLSVAST
                     MYAHGVTAPSRSSVTVQLNRECTRYKAMAGVDDLTLGLGAVRFSVYGGEGARLWQSPVV
                     RGGEPAVPVSVGIEGQETIRLVVEPSGPLGGAALADWAASSISCR"
     gene            complement(685..921)
                     /locus_tag="EAO74_RS39105"
     CDS             complement(685..921)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EAO74_RS39105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_261988505.1"
                     /transl_table=11
                     /translation="MELDQGATAARILGAAGAWRVDSPRSAAEESQVTAATARLHTALG
                     PRRYEEESALGLGLTPDEVLALLTDTAEDLSGG"
     gene            complement(<941..1438)
                     /locus_tag="EAO74_RS36835"
                     /old_locus_tag="EAO74_36895"
                     /pseudo=""
     CDS             complement(<941..1438)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044953.1"
                     /locus_tag="EAO74_RS36835"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="EAO74_36895"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /pseudo=""
                     /sec_met_domain="T1TS (E-value: 1.4e-07, bitscore: 18.8,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRYLILGVTEARDEHGGPLPVGGARLRTLLAALALRAGRPASVAG
                     LIDDVWDDDPPQDAPAALQALVGRLRRVLGREAVAGTPGGYRLTAGPDDIELYVFERLA
                     RQGAAELDAGTPEDAARTLRAALAL"
ORIGIN
        1 ggggaccggc cgcagaatca gcagaagcag cagctcgccc gagcggatcc gaccgacaca
       61 cgaggccaga aattccgtga acttcacgcg ctggagcgcc cgccttcccg gaacgcagcg
      121 tcgagccgcc gcgccgacgc ccacgccgac cccggcaccg cccgccccgg aggagccgcc
      181 gcccgcgccg gaggtctacc aggtgaacca gctggcgtac tccctcaccg gtgaccacac
      241 gggccccgag atcctgctcg gccggagcca gggtgtgttc tggcagcgcc aggggctgtc
      301 ggtcgcctcc acgatgtacg cccacggggt gaccgccccc tcccgctcct ctgtgaccgt
      361 ccagctcaac cgggagtgca cccgctacaa ggcgatggcc ggggtcgacg acctgaccct
      421 ggggctcggc gcggtgcgct tctcggtgta cggcggggag ggggcgcggc tgtggcagtc
      481 cccggtggtc cggggcggtg agcccgccgt accggtgagc gtgggcatcg agggccagga
      541 gacgatccgg ctggtcgtgg agccgtccgg gccgctcggc ggggccgcgc tggccgactg
      601 ggcggcgtcg agcatcagtt gccgctgaga tcctcggcgg cgtgacgtat caactgccgc
      661 cgcgttcccc ggccgcagcg agcctcagcc gccgctgaga tcctcggccg tgtcggtcag
      721 gagggccagg acctcgtccg gtgtcaggcc gaggcccagg gcgctctcct cctcgtaccg
      781 ccgggggccc agcgccgtgt gcagccgtgc cgtcgccgcg gtcacctggg actcctccgc
      841 cgcggagcgg gggctgtcca cccgccaggc gccggccgcc ccgaggatcc gggccgcggt
      901 cgcgccctgg tccagctcca ccagcgaggc cggccgtggc cccgcgccgc aggtccgcct
      961 cgatgcgctg ctggagcgcg gtgagccggt gggcctcggc ctggagcccg tgctcgtgcc
     1021 cggggagatc ggcgagggcg gggccgcgct acagggcgag tgcggcgcgc agggtgcggg
     1081 cggcgtcctc gggggtgccc gcgtcgagct cggcggcgcc ctggcgggcg aggcgctcga
     1141 agacgtacag ctcgatgtcg tcagggccgg cggtgagccg gtagccgccc ggggtgccgg
     1201 ccaccgcctc ccggccgagg acgcgccgca gccgtccgac gagggcttgg agggcggcgg
     1261 gggcgtcctg cggaggatcg tcgtcccata cgtcgtcgat gagcccggcg acgctcgcgg
     1321 ggcggcccgc gcggagggcg agggcggcga gcagggtgcg cagccgggcg ccgccgacgg
     1381 gcagcggccc gccgtgctcg tctcgcgcct cggtgacgcc caggatcaga taccgcaccc
     1441 ggccattgtg tcccgccggg cgcgctgccc gtcggcggcg cccggctgcg caccctgctc
     1501 gccgccctcg cc
//
