LOCUS       NZ_RDBM01000037        10399 bp    DNA     linear   CON 01-JAN-2025
DEFINITION  Streptomyces sp. gb1(2016) Scaffold_37, whole genome shotgun
            sequence.
ACCESSION   NZ_RDBM01000037
VERSION     NZ_RDBM01000037.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. gb1(2016)
  ORGANISM  Streptomyces sp. gb1(2016)
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3233341)
  AUTHORS   Hariharan,J., Choudoir,M.J., Diebold,P., Panke-Buisse,K.,
            Campbell,A.N. and Buckley,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2018) Soil and Crop Sciences, Cornell University,
            306 Tower Road, Ithaca, NY 14850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: A5+MeDuSa v. JUNE-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008042065.1-RS_2025_01_01
            Annotation Date                   :: 01/01/2025 02:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,414
            CDSs (total)                      :: 7,333
            Genes (coding)                    :: 6,887
            CDSs (with protein)               :: 6,887
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 447
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 447
            Pseudo Genes (frameshifted)       :: 94 of 447
            Pseudo Genes (incomplete)         :: 395 of 447
            Pseudo Genes (internal stop)      :: 7 of 447
            Pseudo Genes (multiple problems)  :: 48 of 447
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:17:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2229563
            Orig. end                         :: 2239962
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RDBM01000037.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="4"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(631..1122)
                     /locus_tag="EAO74_RS31745"
                     /old_locus_tag="EAO74_31780"
     CDS             complement(631..1122)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014045198.1"
                     /locus_tag="EAO74_RS31745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31780"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_147985441.1"
                     /transl_table=11
                     /translation="MEQPTSTTLTDQAPADPAAADDALATQPVGYWSGLAHSAVTRHLR
                     DALAGVDVTQPQYWVLNRVRYGLAAPTREELVAELTPLADGPQEISRAVDQLLHRGWIS
                     RDGQRFGLTDAGEAARARLRELVTGLRAEVHRGISDAEYVAALKVLRTMVANIEGARPV
                     "
     gene            complement(1235..2248)
                     /locus_tag="EAO74_RS31750"
                     /old_locus_tag="EAO74_31785"
     CDS             complement(1235..2248)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104671.1"
                     /locus_tag="EAO74_RS31750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31785"
                     /product="VOC family protein"
                     /protein_id="WP_147985442.1"
                     /transl_table=11
                     /translation="MTSLVQHITIDCADAYELALFWAEVLGSQVSDDDAPGDPEALVEA
                     PGAALLFITVPEPKSGKNRIHLDIRPERRTRDEEVERLLALGATLVADHRKPNGRGWAT
                     LADPEGNEFCVECGAAERAALTGTRLPVTADDVTLAVRLAVDTLAASPVDDWHIPAGSL
                     TWDCWETVEHLSDDLFAYAVQLGPRRPSLETEVPYRWAPDREGGPSNSVFADPGAGTAG
                     LLQTLEASGALLTAMVRTASPDLRSYHSHGVSDPEGFAAMGIVETLVHTHDVAAGLSLS
                     WTPPRDLCDRVLARLFPDAPDDEDRWTVLLWSTGRADLPGRARVASWRWHGAPLEG"
     gene            complement(2308..2880)
                     /locus_tag="EAO74_RS31755"
                     /old_locus_tag="EAO74_31790"
     CDS             complement(2308..2880)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405335.1"
                     /locus_tag="EAO74_RS31755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31790"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_403232239.1"
                     /transl_table=11
                     /translation="MQDTRPLTPAGERVVAAAEELFYDRGITAVGVDLIAEHSGVTKRT
                     LYNQFGSKDRLVAAYLAARDRRWQSLVRATVDACDDPVEAVTAPFEALRVWSGTNARGC
                     AFINALAELPDASHPAYVIAADQKRWLVDLFEELATAAGCSAPGALATQLLVLHEGVLA
                     TQPLPLDTLPGSVELARSLVRAAVTRP"
     gene            2953..4002
                     /locus_tag="EAO74_RS31760"
                     /old_locus_tag="EAO74_31795"
     CDS             2953..4002
                     /NRPS_PKS="Domain: PKS_ER (24-347). E-value: 4e-28. Score:
                     90.4. Matches aSDomain:
                     nrpspksdomains_EAO74_RS31760_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018469165.1"
                     /locus_tag="EAO74_RS31760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31795"
                     /product="zinc-binding dehydrogenase"
                     /protein_id="WP_147985443.1"
                     /sec_met_domain="ADH_N (E-value: 4.5e-12, bitscore: 43.3,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 2.2e-25, bitscore:
                     86.7, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVPGTMRAVRIAEHGGPEVLQPSEVAVPAPRAGEVLVRVAAVAL
                     NNTDLWTREGAYGRPGDPEALSGWRGPLDFPRIQGADVAGRVVAVGSGADGSLLGRRVV
                     VDPAIYDSEGPDANPVGLMGSERDGGYAEYVTAPAGRVHDVTDSPLTDDQLATLPTAYG
                     TALGMIERGRLGKGETALVSGASGGVGLALIQIARARGAKVLAISSGSKTDAVREAGAH
                     EVLDRAQDVAEQIRAVAPEGVDVALDVVAGDLVGSVLPLLREGGRWVVAGALGGYEVAF
                     DVRRLYLHNAQVIGSAMHTPTQFGLLMDLARQGTVQPVIAATYPLEQAALAQEALSRRG
                     HVGKIVMHP"
     aSDomain        3025..3993
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS31760_PKS_ER.1"
                     /evalue="4.00E-28"
                     /label="EAO74_RS31760_PKS_ER.1"
                     /locus_tag="EAO74_RS31760"
                     /protein_end="347"
                     /protein_start="24"
                     /score="90.4"
                     /tool="antismash"
                     /translation="EVAVPAPRAGEVLVRVAAVALNNTDLWTREGAYGRPGDPEALSGW
                     RGPLDFPRIQGADVAGRVVAVGSGADGSLLGRRVVVDPAIYDSEGPDANPVGLMGSERD
                     GGYAEYVTAPAGRVHDVTDSPLTDDQLATLPTAYGTALGMIERGRLGKGETALVSGASG
                     GVGLALIQIARARGAKVLAISSGSKTDAVREAGAHEVLDRAQDVAEQIRAVAPEGVDVA
                     LDVVAGDLVGSVLPLLREGGRWVVAGALGGYEVAFDVRRLYLHNAQVIGSAMHTPTQFG
                     LLMDLARQGTVQPVIAATYPLEQAALAQEALSRRGHVGKIVM"
     CDS_motif       3481..3543
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EAO74_RS31760_0001"
                     /evalue="6.30E-06"
                     /label="PKSI-ER_m5"
                     /locus_tag="EAO74_RS31760"
                     /protein_end="197"
                     /protein_start="176"
                     /score="18.7"
                     /tool="antismash"
                     /translation="GETALVSGASGGVGLALIQIA"
     gene            complement(4104..4997)
                     /gene="thpD"
                     /locus_tag="EAO74_RS31765"
                     /old_locus_tag="EAO74_31800"
     CDS             complement(4104..4997)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="EAO74_RS31765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31800"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_147985444.1"
                     /sec_met_domain="PhyH (E-value: 2.9e-38, bitscore: 129.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTDVRADLYPSRGAAEMTTPRQGPVIWSAPGAPGPVTAKDLQGF
                     EENGFLTVDQLITPDEVDVYRAELDRLVADPQVRADERSIIEPQSQSVRSVFEVHKLSQ
                     VFANLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKSFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIKLFTGAAGSATWFDCNCMHGSGDNITPYARSNVFIVFNSVENTAQEPFAAP
                     IRRPDFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="EAO74_RS31770"
                     /old_locus_tag="EAO74_31805"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="EAO74_RS31770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31805"
                     /product="ectoine synthase"
                     /protein_id="WP_099176037.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.3e-53, bitscore:
                     177.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHIKSASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGEKHWISPGTMYLLNGHEHHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            complement(5462..6724)
                     /gene="ectB"
                     /locus_tag="EAO74_RS31775"
                     /old_locus_tag="EAO74_31810"
     CDS             complement(5462..6724)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 3.2e-71.
                     Score: 232.0. Matches aSDomain:
                     nrpspksdomains_EAO74_RS31775_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="EAO74_RS31775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31810"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_147985445.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.4e-76, bitscore:
                     253.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFEALESEVRSYCRSWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFENVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQDLCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKGELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQMEKQTLARGEQVEQTLL
                     AICGEDDTAQFRGRGLVWGLEFTDPERASAVCKRAFELGLLLETSGPQSEVVKLLPPLT
                     VTPDELEEGLRTLARSVRETA"
     aSDomain        complement(5636..6640)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS31775_Aminotran_3.1"
                     /evalue="3.20E-71"
                     /label="EAO74_RS31775_Aminotran_3.1"
                     /locus_tag="EAO74_RS31775"
                     /protein_end="363"
                     /protein_start="28"
                     /score="232.0"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFENVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQDLCHRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKGELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWADGQMEKQTLARGEQVEQTLLAICGEDDTAQFRGRGLVWGLEFT"
     gene            complement(6822..7280)
                     /gene="ectA"
                     /locus_tag="EAO74_RS31780"
                     /old_locus_tag="EAO74_31815"
     CDS             complement(6822..7280)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104675.1"
                     /locus_tag="EAO74_RS31780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31815"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_124277112.1"
                     /transl_table=11
                     /translation="MEDGAAIWRIARDSEVLDLNSSYSYLLWCRDFAATSVVARDETGD
                     PIAFVTGYIRPDRPETLVVWQVAVDRAHRGKGLAAKLLDALTARVASDQGLTSVETTIT
                     PDNAASDRLFTSFAQRHDAPLEHEVLFEGALFPEGTHLPEVLYRIGPF"
     misc_feature    complement(7365..7374)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.52"
                     /tool="antismash"
     misc_feature    complement(7410..7426)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.67"
                     /tool="antismash"
     misc_feature    complement(7501..7516)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7511..7526
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7511..7528)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     gene            complement(7689..8795)
                     /locus_tag="EAO74_RS31785"
                     /old_locus_tag="EAO74_31820"
     CDS             complement(7689..8795)
                     /EC_number="2.6.1.-"
                     /NRPS_PKS="Domain: Aminotran_5 (74-286). E-value: 4.1e-29.
                     Score: 93.6. Matches aSDomain:
                     nrpspksdomains_EAO74_RS31785_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123620.1"
                     /locus_tag="EAO74_RS31785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31820"
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="WP_147985446.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.3e-23, bitscore:
                     79.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLTAARFAAIERRVAGLLATEQDVVIMQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGAEVVDLAVPFHTAVTAEQVRQALAEH
                     PEIDFVSLVHAEAATGNTNPVGEIGEVVRAHGALFMVDAVASVGAEPLLVDDWGVDLCV
                     IGAQKAMGGPAGVSAVSVSTRAWERMAANPGAPRQSYLSLLDWKARWIDGGRRALPHAP
                     AQLEMLALDACLDRIEADGLDTVIARHASAAAATRAGASALGGGLEPYVHDARDAAPVA
                     TTLRVPADLDAAELVGRALATGPSLPLIAGGGALSREMIRVNHYGADATRDAVLSSLAA
                     LGAALADAGRETDLDAAREAVAKTWPMN"
     aSDomain        complement(7938..8573)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS31785_Aminotran_5.1"
                     /evalue="4.10E-29"
                     /label="EAO74_RS31785_Aminotran_5.1"
                     /locus_tag="EAO74_RS31785"
                     /protein_end="286"
                     /protein_start="74"
                     /score="93.6"
                     /tool="antismash"
                     /translation="RDCGAEVVDLAVPFHTAVTAEQVRQALAEHPEIDFVSLVHAEAAT
                     GNTNPVGEIGEVVRAHGALFMVDAVASVGAEPLLVDDWGVDLCVIGAQKAMGGPAGVSA
                     VSVSTRAWERMAANPGAPRQSYLSLLDWKARWIDGGRRALPHAPAQLEMLALDACLDRI
                     EADGLDTVIARHASAAAATRAGASALGGGLEPYVHDARDAAPVATTLRV"
     gene            8899..9999
                     /locus_tag="EAO74_RS31790"
                     /old_locus_tag="EAO74_31825"
     CDS             8899..9999
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="EAO74_RS31790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_31825"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_261988423.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.5e-22, bitscore:
                     77.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDHEAAPVLRIKGRVLVGPDEVRDGLWAVDGRITYERPPGADDA
                     VTLTGWVLPGLVDAHCHVGLDHQGPVDAATSEKQALTDREAGTLLIRDAGSPSDTRWTD
                     AREDLPKIIRAGRHIARTRRYIRNFAHEIEPGDLVAYVAQEARRGDGWVKLVGDWIDRD
                     AGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEDALRDLVEAGIDCIEHATGLTEDTV
                     PLFAERGVAIVPTLVNIATFPGLAAAGEARYPRWSAHMRRLHETRYETVRAAYDAGIPV
                     FTGTDAGGSLAHGLIADEVAELVKAGIPPLDALSATTWGARRWLGRPVLEEGAPADLVM
                     YDEDPRADVRVLASPRAVVLNGRVRG"
     misc_feature    10161..10176
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.99"
                     /tool="antismash"
     misc_feature    10172..10185
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.44"
                     /tool="antismash"
ORIGIN
        1 cgcagctcgc cggcctcctc accgccgggg cggacgcggc gaaggcggtc tcctccacga
       61 cccaggtggc tctgcacctc gccgagggcg gcgacaacgc gggcacccgc tggtggttcg
      121 acaacgcggt ggcgcagaag gtgcccttcg acgtcatcgc cctctcgtac tacgcctact
      181 ggcacggctc cctggccgag ctccagacca acctggacga cgccgcctcg cgctacggca
      241 agccggtcat ggtcgcggag acggcgtacg cccgtacgct cgcgaacgac gacggcctgg
      301 agaacaacgt ggccaccgcg agccagctgg tcaccggcta cccggcgacc ccggccggcc
      361 aggcggcgaa ccttcgcgac gtcatgaacg tcgtggaggc cgtgccgaac ggccggggcc
      421 tgggcgccgt ctactgggaa ccggcctgga ccgccgtcgc cggcagcggc tgggacccgg
      481 ccgacccggc gtcgggcaac gcgtgggaga accaggccgt gttcgaccac gacggcaggc
      541 tgctgccggc ggccggctgg ttctcgcacc ggtagaccgg cgggccgttc cgacggcccg
      601 gagtgtgccc cgtccgggcc gtcgcacctc tcagacgggg cgcgcgccct cgatgttggc
      661 caccatcgtc cggagcacct tcagcgcggc cacgtactcc gcgtcgctga tcccccggtg
      721 gacctcggcg cggagaccgg tcaccagctc acgcagccgc gcccgggcgg cctccccggc
      781 gtcggtgagg ccgaaccgct gcccgtcccg gctgatccat ccccggtgga gcagctggtc
      841 gacggccctg gagatctcct gcggcccgtc ggccaggggc gtgagctcgg cgaccagctc
      901 ctcacgggtg ggcgcggcga gtccgtagcg cacccggttg agcacccagt actggggctg
      961 cgtgacatcg accccggcca gggcgtcccg cagatgccgg gtgacggcgg agtgggccag
     1021 gccgctccag tacccgacgg gctgtgtggc cagggcgtcg tccgccgccg ccgggtccgc
     1081 gggcgcctga tcggtgaggg tggtgctggt cggctgctcc atggccgtga cgctaccggc
     1141 gcgccccggc cggcggcccc ggaggcaccg gaaccggccg gaacgcggcc ggttccggcg
     1201 gctcagggct ccggcggccg ggcgatccgg tgactcaccc ctccagcggc gcgccgtgcc
     1261 acctccacga ggcgacgcgg gcgcgccccg gcagatccgc gcggccggtg gaccacagca
     1321 ggacggtcca ccggtcctcg tcgtccgggg cgtcggggaa cagccgggcc agcacgcggt
     1381 cgcagaggtc ccggggcggg gtccaggaga gggacagccc ggcggcgacg tcatgggtgt
     1441 ggaccagggt ctcgacgatg cccatcgcgg cgaagccctc cgggtcggag acgccgtgcg
     1501 agtggtacga acggaggtcg ggggaggccg tccgcaccat cgctgtcagc agcgcgccgc
     1561 tcgcttccag cgtctggagc aggccggcgg tccccgcccc ggggtccgcg aagaccgagt
     1621 tcgaggggcc gccctcgcgg tccggggccc agcggtaggg gacctcggtc tccagggagg
     1681 gcctgcgcgg gccgagctgg acggcgtacg cgaagaggtc gtcgctcagg tgctccacgg
     1741 tctcccagca gtcccacgtc aacgacccgg ccgggatatg ccagtcgtcc accggggacg
     1801 ccgcgagggt gtcgaccgcg agccgtaccg ccagcgtcac gtcgtcggcg gtgaccggca
     1861 gccgtgtgcc ggtcagcgcc gcgcgctcgg ctgccccgca ctccacgcag aactcgttgc
     1921 cctccggatc ggccagcgtc gcccagcccc ggccgttcgg cttgcggtgg tccgcgacca
     1981 gcgtggcgcc cagggcgagc agccgctcga cctcctcgtc gcgggtacgc cgctcgggac
     2041 ggatgtccag atggatccgg ttcttgccgc tcttgggttc cggcacggtg atgaagagca
     2101 gcgcggcgcc cggcgcctcc acgagcgcct cgggatcgcc gggcgcgtcg tcgtcggaga
     2161 cctgcgagcc gagtacctcg gcccagaaga gggccagttc gtaggcgtcg gcacagtcga
     2221 tggtgatgtg ctgtacgagc gaagtcatgg acgccactgt ctccgctgat ccgtgccggg
     2281 tccagcgagt tgtacggtcc ggtggcccta cgggcgggtc acggccgccc gcaccaggga
     2341 ccgcgccagc tcgacgctgc ccgggagggt gtcgagcggg aggggctgcg tggcgaggac
     2401 gccctcgtgc agcacgagga gctgggtggc cagggcgccg ggggccgagc agcccgcagc
     2461 ggtggcgagc tcctcgaaca ggtcgacgag ccagcgcttc tggtccgcgg cgatgacgta
     2521 cgcgggatgg gaggcgtccg ggagctcggc cagggcgttg atgaacgcgc atccccgggc
     2581 gttggtcccg ctccagaccc gcagtgcctc gaagggggcg gtgacggcct cgaccgggtc
     2641 gtcgcaggcg tcgacggtgg cgcggacgag cgactgccag cgccggtcgc gtgccgcgag
     2701 gtaggcggcc acgagccggt ccttggagcc gaactggttg tacagggtcc gcttggtcac
     2761 ccccgagtgc tcggcgatca gatccacgcc gaccgccgtg atgccgcggt cgtagaacag
     2821 ctcctcggcg gccgcgacga cgcgctcgcc ggccggcgtc agggggcgtg tgtcctgcat
     2881 cagcattcct tcaccgatcg gtgtaccgtg gagtgtgttc acagatcagt atacctagca
     2941 ggaggtcgag tgatggacgt gcccggaacc atgcgcgcag tgcggatcgc ggagcacggg
     3001 gggcccgagg tcctccagcc gtcagaggtc gccgtgcccg cgccgcgggc gggagaggtg
     3061 ctggtcaggg tcgccgcggt ggcgctgaac aacaccgacc tgtggacccg tgagggcgcc
     3121 tacggccggc ccggagaccc ggaggcgctg tccggctggc gaggcccgct cgacttcccg
     3181 cgcatccagg gcgccgacgt ggccggccgg gtcgtggccg tcggttccgg cgcggacggg
     3241 agcctcctcg gccgccgggt ggtcgtcgac ccggcgatct acgactcgga aggaccggac
     3301 gcgaacccgg tgggcctgat ggggagcgaa cgcgacggcg gttacgccga gtacgtcacc
     3361 gcgcccgccg ggcgtgtgca cgacgtgacg gactccccgc tcaccgacga ccagctcgcg
     3421 acgctgccga ccgcctacgg cacggcgctg ggcatgatcg agcggggcag gctgggcaag
     3481 ggcgagaccg cgctggtctc gggggcgtcc gggggagtgg gcctcgcgct gatccagatc
     3541 gcccgggccc gcggtgcgaa ggtgctcgcc atcagcagcg gatccaagac cgacgcggtg
     3601 cgtgaggcgg gcgcccacga ggtcctcgac cgggcgcagg acgtcgccga gcagatccgc
     3661 gccgtcgccc cggagggcgt ggacgtcgca ctggacgtcg tcgccggtga cctggtgggc
     3721 tcggtgctgc cgctgctgcg cgagggcggc cgatgggtcg tcgccggtgc gctcggaggg
     3781 tacgaggtgg ccttcgacgt acgccggctc tatctgcaca acgcgcaggt gatcggctcc
     3841 gcgatgcaca cgcccacgca gttcggcctc ctcatggacc tggcccggca gggcacggtc
     3901 cagcccgtca tcgccgcgac ctaccccctg gagcaggccg ccctggcaca ggaggcgctc
     3961 tcccgcaggg gccacgtggg aaagatcgtc atgcacccgt gatcgtcatg cacccatgag
     4021 gggagaacgt cccttgcgcg gcacaggacg aggacggggc gggacgcgtg atgcgtcccg
     4081 ccccgtcctc gtgccgtacg tccctacttc accggcgtga agtcacgcgc cccgatgaag
     4141 tcgggccggc ggatcggagc cgcgaagggc tcctgcgccg tgttctccac gctgttgaag
     4201 acgatgaaga cgttgctgcg cgcgtacggg gtgatgttgt cccccgagcc gtgcatgcag
     4261 ttgcagtcga accaggtcgc cgaaccggcc gcgcccgtga agagcttgat gccgtgccgg
     4321 tcggccatct tcgtcagcgc ctcgtcggac ggggtgccgg cgtcctgcat ctgcagggac
     4381 ttcttgtagt tgtccttcgg cgtctcgccc gcacagccga ggaacgactt gtgcgaaccg
     4441 ggcatgatca tcagcccgcc gttggtgtcg aagttctcgg tcagcgcgat cgagaccgac
     4501 acggtccgca tgttcggcag accgtcctcg gcgtgccagg tctcgaagtc cgagtgccag
     4561 tagaagcccg aggcaccgaa gcccggcttg acgttgatcc ttgactggtg gacgtacacg
     4621 tcggagccga ggatctggcg ggcacggccc accacacgct cgtcgcggac caggttcgcg
     4681 aagacctggc tgagcttgtg gacctcgaag acggaccgca cgctctgcga ctgcggttcg
     4741 atgatcgagc gctcgtcggc ccggacctgc gggtcggcga ccagccggtc cagctcggcc
     4801 cggtagacgt ccacctcgtc cggagtgatg agctggtcga cggtgaggaa gccgttctcc
     4861 tcgaagccct ggagatcctt ggcggtgacc ggcccgggcg cgcccggcgc ggaccagatg
     4921 accgggccct ggcggggagt ggtcatctcg gcggcgccgc gcgaggggta caggtcggcg
     4981 cgtacatcgg tggtcatggt tcagccctcc tctgtcagca gcgggtatac accgttctcg
     5041 tcatggtcct cccgcccggt gacgggggga ttgaagacgc acacgcagcg gaagtcggtc
     5101 ttcggccgca gcgtgtggtg ctcgtgcccg ttcagcaggt acatcgtccc gggggagatc
     5161 cagtgcttct cgccggtctc gtcgttggtg agctcggcct cgccctcggt gcacagaacg
     5221 gcctcgatgt ggttcgcgta ccacatcgac gtctccgtgc ccgcgtacag cacggtctcg
     5281 tggagagaga agcccacctt ctccttggcg agcacgatgc gcttgctctc ccaggtacct
     5341 gatgcggact tgatgtgccg gtcggtgttc tcgatgtcac tgaaagatcg gacgatcacg
     5401 gtggggtggt gcctttctct tctgtcctgc tctgtggtgc ctacgcgggg gagtgccccg
     5461 ctcaggcggt ctctcggacg gagcgggcca gcgtgcgcag gccctcctcc agctcatcgg
     5521 gggtgacggt cagcggcggg agcagcttca cgacctcgga ctgcgggccg gaggtctcca
     5581 gcagcaggcc cagctcgaag gcgcgcttgc agacggcgga cgcgcgctcg gggtcggtga
     5641 actccaggcc ccagaccagg ccgcggccgc ggaactgcgc ggtgtcgtcc tcgccgcaga
     5701 tcgccagcag cgtctgctcg acctgctcgc cgcgggccag ggtctgcttc tccatctggc
     5761 cgtcggccca gtaggcgtcg agcgcggcgg cggcggtgac gaacgccggg ttgttgccgc
     5821 ggaaggtgcc gttgtgctcg cccggctccc agatgtccag ctcgcccttg aagaggcaga
     5881 gcgacatcgg caggccgtag ccgctgatgg acttcgacag cgtcacgatg tccgggacga
     5941 tgccggcctc ctcgaaggag aagaagccgc cggtccggcc gcatcccatc tggatgtcgt
     6001 cgacgatcag gagcatgtcc tggcggtggc agaggtcctg gagggcgcgc agccactcgg
     6061 cgcgggcgac gttgatcccg ccctcgccct gcacggtctc cacgatcacg gcggcgggct
     6121 tgttcagccc ggaaccctgg tcctccagga gccgctcgaa ccagaggaag tcgggcacct
     6181 ggccgtcgaa gtagttgtcg aacggcatcg gggtgccgtg gaccagcggg atgccggcgc
     6241 cggcccgctt gaaggcgttg ccggtcacgg cgagcgagcc gagcgacatg ccgtggaagg
     6301 cgttggtgaa cgagacgacg gactcgcggc ccttgacctt gcgggcgagc ttcagcgccg
     6361 actcgacggc gttggtgccc gtcgggccgg ggaacatcac cttgtagggc aggtcacgcg
     6421 ggcgcaggat cacgttctcg aaggtctcga ggaacgcccg cttggccgtc gtggccatgt
     6481 ccaggccgtg ggtgatgccg tcgcgctcga tgtagtcgat gagcgcgcgt ttcagcaccg
     6541 ggttgttgtg cccgtagttg agagatccgg caccggcgaa gaagtcgagg tacgagtggc
     6601 cgtcctcgtc ggtcaggcgg gcgccctgcg cgcggtcgaa gaccgcgggc caactgcggc
     6661 agtagctccg tacttccgac tccagggctt cgaagacgct cagggcgggc ggggtgatgg
     6721 tcacagctga tctcctgctc gaggggggtg tgacgggcgg cgggtcaggc atgcccggga
     6781 aagccgcgcc cgggcggggc gcggtagggc gctgggaggc cctagaacgg gccgatgcgg
     6841 tagagaacct ccggcaggtg cgtcccctcg gggaacagcg ccccctcgaa gagcacctcg
     6901 tgctccagcg gcgcgtcgtg ccgctgcgcg aaggacgtga acagccggtc ggaggcggcg
     6961 ttgtccgggg tgatcgtcgt ctcaaccgat gtcagaccct ggtcggaggc gacacgggcg
     7021 gtcagtgcgt ccagcagctt ggccgccagt cccttgccac ggtgggcccg gtcgacggcc
     7081 acctgccaga cgacgagcgt ctcggggcgg tcggggcgga tgtatccggt cacgaaggcg
     7141 atggggtcgc cggtctcgtc gcgggcgacc acggaggtcg ccgcgaagtc acggcaccac
     7201 agcaggtagc tgtacgagga gttgaggtcc aggacctcgg agtcgcgggc aatgcgccag
     7261 atcgcggctc cgtcctccac tcgcggggtg tcgattctga ggaattcgct tcgggcactt
     7321 gcaaaatctg ctggtgcggc ggtcatgcga attgaattta cccagcaaaa atcaaaattg
     7381 catcgccgca gggggttggg tcggcgcgct cgttgtgtta tcacgcgggc gcggatccac
     7441 ctcgatcgag gggtgtttta gccggtcctg tccggaattt aacgcccaaa gcggactcga
     7501 tgtggggtcg gtcacatcgt tgtaactctc ctgacatccg tccggatcgg ctgggccgat
     7561 cgagtgaaaa ctgtggcgtt tagcctctga gaacggggga agaagaatgc ggggagctgc
     7621 tcccgataaa gcagctcccc ttttcgtgtc cgcgtccgat aaaccggccg ctgtagttcg
     7681 catttaattc agttcatcgg ccaggttttc gcgacggctt cgcgggcagc atcgaggtcg
     7741 gtctcccgtc ccgcgtccgc cagtgcggcg cccagggcgg ccagcgagga cagcaccgcg
     7801 tcccgcgtcg cgtccgcgcc gtagtggttg acccggatca tctccctgga cagcgcgccg
     7861 ccacccgcga tcagcggcag cgagggcccg gtcgcgagcg cccggccgac cagctccgcc
     7921 gcgtcgagat ccgccggtac gcgcagggtc gtggcgacgg gcgccgcgtc ccgggcgtca
     7981 tggacgtacg gctccagccc gccgccgagc gccgaggcgc ccgcccgggt cgccgcggcg
     8041 gccgacgcgt gccgcgcgat gaccgtgtcc aggccgtccg cctcgatccg gtccaggcag
     8101 gcgtcgagcg ccagcatctc cagctgggcg ggcgcgtgcg gcagcgccct gcggcccccg
     8161 tcgatccagc gggccttcca gtccagcagc gagaggtacg actgccgcgg agcccccggg
     8221 ttggcggcca tccgctccca cgcccgcgtg ctcaccgaca cggcggagac cccggccggg
     8281 ccgcccatgg ccttctgcgc gccgatgacg cacaggtcca cgccccagtc gtccaccagc
     8341 agcggctccg cacccaccga cgcgacggcg tccaccatga acagcgcgcc gtgtgcccgg
     8401 accacctcgc cgatctcacc gaccgggttg gtgttgccgg tcgccgcctc ggcgtgcacg
     8461 agcgacacga agtcgatctc cgggtgctcg gccagtgcct gccggacctg ctcggcggtg
     8521 accgcggtgt ggaacggcac cgccaggtcc acgacctcgg cgccgcagtc gcgcagccag
     8581 ttgccgaagg tctggccgta cggcccggtc acgacgttca gcgcggtcga gccgggccgg
     8641 gcgccgcccc ggatgcagcc ttcgaggggc agcagcgcct cgccctgcat gatgacgacg
     8701 tcctgctcgg tggcgaggag gccggccacc cgccgctcga tcgctgcgaa acgcgcggcg
     8761 gtgagcgggg cgaggtcgag gaatgggtgt gtcacggtgc tgctctcttc gggtcggccg
     8821 gtgcgctgtc agcgcctgcg cgtcgagggt acccagcacc ctccctggcc cccgcggcct
     8881 cctcgtacag tgctgtccat gagcgatcac gaggcggcgc cggtgctgcg gatcaagggg
     8941 cgggtgctcg tcgggcccga cgaggtgagg gacggcctct gggcggtcga cgggcggatc
     9001 acctacgagc ggccgccggg cgcggacgac gccgtgacgc tcaccggctg ggtgctgccc
     9061 ggtctggtcg acgcgcactg ccatgtgggg ctcgaccacc aggggcccgt cgacgcggcc
     9121 accagcgaga agcaggccct caccgaccgt gaggccggca ccctgctcat ccgggacgcc
     9181 gggtcaccct ccgacacccg gtggacggac gcgcgcgagg acctgccgaa gatcatcagg
     9241 gcggggcgtc acatcgccag gacgcgccgt tacatccgca acttcgccca tgagatcgag
     9301 cccggtgacc tggtcgccta cgtcgcccag gaggcccggc gcggcgacgg ctgggtcaag
     9361 ctggtcggcg actggatcga ccgggacgcc ggcgacctga ccgcctgctg gccgcgcggc
     9421 gaggtcgagg ccgcgatcgc cgaggcccac cggctcggcg cccgggtcac cgcccactgc
     9481 ttcgccgagg acgcgctgcg cgacctggtg gaggccggga tcgactgcat cgagcacgcg
     9541 acgggcctca ccgaggacac cgtcccgctc ttcgcggagc gcggtgtcgc gatcgtcccc
     9601 accctggtca acatcgccac cttccccgga ctcgcggcgg ccggcgaggc caggtacccc
     9661 cgctggtccg cccatatgcg gcggctccac gagacccgct acgagaccgt gcgcgccgcc
     9721 tacgacgcgg gcatcccggt cttcaccggc accgacgcgg gcggctcgct ggcccacggc
     9781 ctgatcgcgg acgaggtggc cgaactggtg aaggccggca tcccgcccct ggacgcgctc
     9841 tccgcgacca cctggggcgc ccggcggtgg ctgggacggc cggtgctgga ggagggcgcc
     9901 ccggcggacc tggtgatgta cgacgaggac ccgagggcgg acgtacgggt gctggcgtcc
     9961 ccccgcgcgg tggtgctcaa tgggcgggtg aggggctgat cgccggttga cccgctcagg
    10021 ggcgccgtgt tgaccggcgg tgaccccggg cgccgcgtct cgttgaacca cctctgtgct
    10081 cggcctgggg catcccgtga tcatgccctc gaagcagtga aacgggtggt tccgaggaac
    10141 tcctgtgcca gtggattcga atgtgcacac ggaaacccct ctttggagtg aactggcgga
    10201 aggtgtctgc cagttcaccc tctgtgcgta aagattcacg gagtcgaggc caccggtgcc
    10261 cgcgatgtcc cccattggac ggctccggtg gctccatgtc tcttgtgggg gttccaccat
    10321 cttgaacagc aacaccttcc gttcggctgc cctggcgatc gccgccgctc ccgtcgccct
    10381 gctcgtcgcc gtccccgcg
//
