LOCUS NZ_RDBM01000037 10399 bp DNA linear CON 01-JAN-2025 DEFINITION Streptomyces sp. gb1(2016) Scaffold_37, whole genome shotgun sequence. ACCESSION NZ_RDBM01000037 VERSION NZ_RDBM01000037.1 KEYWORDS WGS; RefSeq. SOURCE Streptomyces sp. gb1(2016) ORGANISM Streptomyces sp. gb1(2016) Bacteria; Bacillati; Actinomycetota; Actinomycetes; Kitasatosporales; Streptomycetaceae; Streptomyces. REFERENCE 1 (bases 1 to 3233341) AUTHORS Hariharan,J., Choudoir,M.J., Diebold,P., Panke-Buisse,K., Campbell,A.N. and Buckley,D.H. TITLE Direct Submission JOURNAL Submitted (16-OCT-2018) Soil and Crop Sciences, Cornell University, 306 Tower Road, Ithaca, NY 14850, USA COMMENT ##Genome-Assembly-Data-START## Assembly Date :: 2018 Assembly Method :: A5+MeDuSa v. JUNE-2018 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 66x Sequencing Technology :: Illumina MiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_008042065.1-RS_2025_01_01 Annotation Date :: 01/01/2025 02:23:01 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.9 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 7,414 CDSs (total) :: 7,333 Genes (coding) :: 6,887 CDSs (with protein) :: 6,887 Genes (RNA) :: 81 rRNAs :: 6, 1, 2 (5S, 16S, 23S) complete rRNAs :: 6, 1, 1 (5S, 16S, 23S) partial rRNAs :: 1 (23S) tRNAs :: 69 ncRNAs :: 3 Pseudo Genes (total) :: 447 CDSs (without protein) :: 446 Pseudo Genes (ambiguous residues) :: 0 of 447 Pseudo Genes (frameshifted) :: 94 of 447 Pseudo Genes (incomplete) :: 395 of 447 Pseudo Genes (internal stop) :: 7 of 447 Pseudo Genes (multiple problems) :: 48 of 447 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-13 13:17:57 NOTE :: This is a single region extracted from a larger record! Orig. start :: 2229563 Orig. end :: 2239962 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to RDBM01000037.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ FEATURES Location/Qualifiers region 1..10399 /candidate_cluster_numbers="1" /contig_edge="False" /product="ectoine" /region_number="4" /rules="ectoine_synt" /tool="antismash" cand_cluster 1..10399 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="ectoine_synt" /kind="single" /product="ectoine" /protoclusters="1" /tool="antismash" protocluster 1..10399 /aStool="rule-based-clusters" /category="other" /contig_edge="False" /core_location="[5000:5399](-)" /cutoff="20000" /detection_rule="ectoine_synt" /neighbourhood="5000" /product="ectoine" /protocluster_number="1" /tool="antismash" proto_core complement(5001..5399) /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="ectoine_synt" /neighbourhood="5000" /product="ectoine" /protocluster_number="1" gene complement(631..1122) /locus_tag="EAO74_RS31745" /old_locus_tag="EAO74_31780" CDS complement(631..1122) /GO_function="GO:0003700 - DNA-binding transcription factor activity [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014045198.1" /locus_tag="EAO74_RS31745" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31780" /product="MarR family winged helix-turn-helix transcriptional regulator" /protein_id="WP_147985441.1" /transl_table=11 /translation="MEQPTSTTLTDQAPADPAAADDALATQPVGYWSGLAHSAVTRHLR DALAGVDVTQPQYWVLNRVRYGLAAPTREELVAELTPLADGPQEISRAVDQLLHRGWIS RDGQRFGLTDAGEAARARLRELVTGLRAEVHRGISDAEYVAALKVLRTMVANIEGARPV " gene complement(1235..2248) /locus_tag="EAO74_RS31750" /old_locus_tag="EAO74_31785" CDS complement(1235..2248) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018104671.1" /locus_tag="EAO74_RS31750" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31785" /product="VOC family protein" /protein_id="WP_147985442.1" /transl_table=11 /translation="MTSLVQHITIDCADAYELALFWAEVLGSQVSDDDAPGDPEALVEA PGAALLFITVPEPKSGKNRIHLDIRPERRTRDEEVERLLALGATLVADHRKPNGRGWAT LADPEGNEFCVECGAAERAALTGTRLPVTADDVTLAVRLAVDTLAASPVDDWHIPAGSL TWDCWETVEHLSDDLFAYAVQLGPRRPSLETEVPYRWAPDREGGPSNSVFADPGAGTAG LLQTLEASGALLTAMVRTASPDLRSYHSHGVSDPEGFAAMGIVETLVHTHDVAAGLSLS WTPPRDLCDRVLARLFPDAPDDEDRWTVLLWSTGRADLPGRARVASWRWHGAPLEG" gene complement(2308..2880) /locus_tag="EAO74_RS31755" /old_locus_tag="EAO74_31790" CDS complement(2308..2880) /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_function="GO:0003700 - DNA-binding transcription factor activity [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1057: TetR family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008405335.1" /locus_tag="EAO74_RS31755" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31790" /product="TetR/AcrR family transcriptional regulator" /protein_id="WP_403232239.1" /transl_table=11 /translation="MQDTRPLTPAGERVVAAAEELFYDRGITAVGVDLIAEHSGVTKRT LYNQFGSKDRLVAAYLAARDRRWQSLVRATVDACDDPVEAVTAPFEALRVWSGTNARGC AFINALAELPDASHPAYVIAADQKRWLVDLFEELATAAGCSAPGALATQLLVLHEGVLA TQPLPLDTLPGSVELARSLVRAAVTRP" gene 2953..4002 /locus_tag="EAO74_RS31760" /old_locus_tag="EAO74_31795" CDS 2953..4002 /NRPS_PKS="Domain: PKS_ER (24-347). E-value: 4e-28. Score: 90.4. Matches aSDomain: nrpspksdomains_EAO74_RS31760_PKS_ER.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) ADH_N" /gene_functions="biosynthetic-additional (rule-based-clusters) ADH_zinc_N" /gene_functions="biosynthetic-additional (smcogs) SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018469165.1" /locus_tag="EAO74_RS31760" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31795" /product="zinc-binding dehydrogenase" /protein_id="WP_147985443.1" /sec_met_domain="ADH_N (E-value: 4.5e-12, bitscore: 43.3, seeds: 66, tool: rule-based-clusters)" /sec_met_domain="ADH_zinc_N (E-value: 2.2e-25, bitscore: 86.7, seeds: 86, tool: rule-based-clusters)" /transl_table=11 /translation="MDVPGTMRAVRIAEHGGPEVLQPSEVAVPAPRAGEVLVRVAAVAL NNTDLWTREGAYGRPGDPEALSGWRGPLDFPRIQGADVAGRVVAVGSGADGSLLGRRVV VDPAIYDSEGPDANPVGLMGSERDGGYAEYVTAPAGRVHDVTDSPLTDDQLATLPTAYG TALGMIERGRLGKGETALVSGASGGVGLALIQIARARGAKVLAISSGSKTDAVREAGAH EVLDRAQDVAEQIRAVAPEGVDVALDVVAGDLVGSVLPLLREGGRWVVAGALGGYEVAF DVRRLYLHNAQVIGSAMHTPTQFGLLMDLARQGTVQPVIAATYPLEQAALAQEALSRRG HVGKIVMHP" aSDomain 3025..3993 /ASF="ER configuration inconclusive" /aSDomain="PKS_ER" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_EAO74_RS31760_PKS_ER.1" /evalue="4.00E-28" /label="EAO74_RS31760_PKS_ER.1" /locus_tag="EAO74_RS31760" /protein_end="347" /protein_start="24" /score="90.4" /tool="antismash" /translation="EVAVPAPRAGEVLVRVAAVALNNTDLWTREGAYGRPGDPEALSGW RGPLDFPRIQGADVAGRVVAVGSGADGSLLGRRVVVDPAIYDSEGPDANPVGLMGSERD GGYAEYVTAPAGRVHDVTDSPLTDDQLATLPTAYGTALGMIERGRLGKGETALVSGASG GVGLALIQIARARGAKVLAISSGSKTDAVREAGAHEVLDRAQDVAEQIRAVAPEGVDVA LDVVAGDLVGSVLPLLREGGRWVVAGALGGYEVAFDVRRLYLHNAQVIGSAMHTPTQFG LLMDLARQGTVQPVIAATYPLEQAALAQEALSRRGHVGKIVM" CDS_motif 3481..3543 /aSTool="nrps_pks_domains" /database="abmotifs" /detection="hmmscan" /domain_id="nrpspksmotif_EAO74_RS31760_0001" /evalue="6.30E-06" /label="PKSI-ER_m5" /locus_tag="EAO74_RS31760" /protein_end="197" /protein_start="176" /score="18.7" /tool="antismash" /translation="GETALVSGASGGVGLALIQIA" gene complement(4104..4997) /gene="thpD" /locus_tag="EAO74_RS31765" /old_locus_tag="EAO74_31800" CDS complement(4104..4997) /EC_number="1.14.11.55" /GO_function="GO:0016706 - 2-oxoglutarate-dependent dioxygenase activity [Evidence IEA]" /codon_start=1 /gene="thpD" /gene_functions="biosynthetic-additional (rule-based-clusters) PhyH" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014156966.1" /locus_tag="EAO74_RS31765" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31800" /product="ectoine hydroxylase" /protein_id="WP_147985444.1" /sec_met_domain="PhyH (E-value: 2.9e-38, bitscore: 129.7, seeds: 39, tool: rule-based-clusters)" /transl_table=11 /translation="MTTDVRADLYPSRGAAEMTTPRQGPVIWSAPGAPGPVTAKDLQGF EENGFLTVDQLITPDEVDVYRAELDRLVADPQVRADERSIIEPQSQSVRSVFEVHKLSQ VFANLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM RTVSVSIALTENFDTNGGLMIMPGSHKSFLGCAGETPKDNYKKSLQMQDAGTPSDEALT KMADRHGIKLFTGAAGSATWFDCNCMHGSGDNITPYARSNVFIVFNSVENTAQEPFAAP IRRPDFIGARDFTPVK" gene complement(5001..5399) /locus_tag="EAO74_RS31770" /old_locus_tag="EAO74_31805" CDS complement(5001..5399) /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) ectoine: ectoine_synt" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_006123624.1" /locus_tag="EAO74_RS31770" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31805" /product="ectoine synthase" /protein_id="WP_099176037.1" /sec_met_domain="ectoine_synt (E-value: 1.3e-53, bitscore: 177.7, seeds: 10, tool: rule-based-clusters)" /transl_table=11 /translation="MIVRSFSDIENTDRHIKSASGTWESKRIVLAKEKVGFSLHETVLY AGTETSMWYANHIEAVLCTEGEAELTNDETGEKHWISPGTMYLLNGHEHHTLRPKTDFR CVCVFNPPVTGREDHDENGVYPLLTEEG" gene complement(5462..6724) /gene="ectB" /locus_tag="EAO74_RS31775" /old_locus_tag="EAO74_31810" CDS complement(5462..6724) /EC_number="2.6.1.76" /GO_function="GO:0030170 - pyridoxal phosphate binding [Evidence IEA]" /GO_function="GO:0047307 - diaminobutyrate-pyruvate transaminase activity [Evidence IEA]" /GO_process="GO:0019491 - ectoine biosynthetic process [Evidence IEA]" /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 3.2e-71. Score: 232.0. Matches aSDomain: nrpspksdomains_EAO74_RS31775_Aminotran_3.1" /codon_start=1 /gene="ectB" /gene_functions="biosynthetic-additional (rule-based-clusters) Aminotran_3" /gene_functions="biosynthetic-additional (smcogs) SMCOG1013: aminotransferase class-III" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014156968.1" /locus_tag="EAO74_RS31775" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31810" /product="diaminobutyrate--2-oxoglutarate transaminase" /protein_id="WP_147985445.1" /sec_met_domain="Aminotran_3 (E-value: 6.4e-76, bitscore: 253.1, seeds: 13, tool: rule-based-clusters)" /transl_table=11 /translation="MTITPPALSVFEALESEVRSYCRSWPAVFDRAQGARLTDEDGHSY LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFENVILRPRD LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA EWLRALQDLCHRQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS LCLFKGELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWADGQMEKQTLARGEQVEQTLL AICGEDDTAQFRGRGLVWGLEFTDPERASAVCKRAFELGLLLETSGPQSEVVKLLPPLT VTPDELEEGLRTLARSVRETA" aSDomain complement(5636..6640) /aSDomain="Aminotran_3" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_EAO74_RS31775_Aminotran_3.1" /evalue="3.20E-71" /label="EAO74_RS31775_Aminotran_3.1" /locus_tag="EAO74_RS31775" /protein_end="363" /protein_start="28" /score="232.0" /tool="antismash" /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE RDGITHGLDMATTAKRAFLETFENVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQDLCHRQDMLLIVDDIQMGCGR TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKGELDIWEPGEHNGTFRGNNPAFV TAAAALDAYWADGQMEKQTLARGEQVEQTLLAICGEDDTAQFRGRGLVWGLEFT" gene complement(6822..7280) /gene="ectA" /locus_tag="EAO74_RS31780" /old_locus_tag="EAO74_31815" CDS complement(6822..7280) /EC_number="2.3.1.178" /GO_function="GO:0033816 - diaminobutyrate acetyltransferase activity [Evidence IEA]" /GO_process="GO:0019491 - ectoine biosynthetic process [Evidence IEA]" /codon_start=1 /gene="ectA" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018104675.1" /locus_tag="EAO74_RS31780" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31815" /product="diaminobutyrate acetyltransferase" /protein_id="WP_124277112.1" /transl_table=11 /translation="MEDGAAIWRIARDSEVLDLNSSYSYLLWCRDFAATSVVARDETGD PIAFVTGYIRPDRPETLVVWQVAVDRAHRGKGLAAKLLDALTARVASDQGLTSVETTIT PDNAASDRLFTSFAQRHDAPLEHEVLFEGALFPEGTHLPEVLYRIGPF" misc_feature complement(7365..7374) /note="TFBS match to ToxT, Regulator of MSHA biosynthesis, confidence: weak, score: 15.52" /tool="antismash" misc_feature complement(7410..7426) /note="TFBS match to ZuR_variant_2, Zinc-responsive repressor, confidence: weak, score: 16.67" /tool="antismash" misc_feature complement(7501..7516) /note="TFBS match to CRP, Cyclic AMP receptor protein, confidence: weak, score: 16.03" /tool="antismash" misc_feature 7511..7526 /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2, activator of antibiotic production, confidence: medium, score: 17.62" /tool="antismash" misc_feature complement(7511..7528) /note="TFBS match to ArcA, Repressor of carbon oxidation, confidence: weak, score: 14.75" /tool="antismash" gene complement(7689..8795) /locus_tag="EAO74_RS31785" /old_locus_tag="EAO74_31820" CDS complement(7689..8795) /EC_number="2.6.1.-" /NRPS_PKS="Domain: Aminotran_5 (74-286). E-value: 4.1e-29. Score: 93.6. Matches aSDomain: nrpspksdomains_EAO74_RS31785_Aminotran_5.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Aminotran_5" /gene_functions="biosynthetic-additional (smcogs) SMCOG1139: aminotransferase class V" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_006123620.1" /locus_tag="EAO74_RS31785" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31820" /product="pyridoxal-phosphate-dependent aminotransferase family protein" /protein_id="WP_147985446.1" /sec_met_domain="Aminotran_5 (E-value: 3.3e-23, bitscore: 79.7, seeds: 34, tool: rule-based-clusters)" /transl_table=11 /translation="MTHPFLDLAPLTAARFAAIERRVAGLLATEQDVVIMQGEALLPLE GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGAEVVDLAVPFHTAVTAEQVRQALAEH PEIDFVSLVHAEAATGNTNPVGEIGEVVRAHGALFMVDAVASVGAEPLLVDDWGVDLCV IGAQKAMGGPAGVSAVSVSTRAWERMAANPGAPRQSYLSLLDWKARWIDGGRRALPHAP AQLEMLALDACLDRIEADGLDTVIARHASAAAATRAGASALGGGLEPYVHDARDAAPVA TTLRVPADLDAAELVGRALATGPSLPLIAGGGALSREMIRVNHYGADATRDAVLSSLAA LGAALADAGRETDLDAAREAVAKTWPMN" aSDomain complement(7938..8573) /aSDomain="Aminotran_5" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_EAO74_RS31785_Aminotran_5.1" /evalue="4.10E-29" /label="EAO74_RS31785_Aminotran_5.1" /locus_tag="EAO74_RS31785" /protein_end="286" /protein_start="74" /score="93.6" /tool="antismash" /translation="RDCGAEVVDLAVPFHTAVTAEQVRQALAEHPEIDFVSLVHAEAAT GNTNPVGEIGEVVRAHGALFMVDAVASVGAEPLLVDDWGVDLCVIGAQKAMGGPAGVSA VSVSTRAWERMAANPGAPRQSYLSLLDWKARWIDGGRRALPHAPAQLEMLALDACLDRI EADGLDTVIARHASAAAATRAGASALGGGLEPYVHDARDAAPVATTLRV" gene 8899..9999 /locus_tag="EAO74_RS31790" /old_locus_tag="EAO74_31825" CDS 8899..9999 /GO_function="GO:0016787 - hydrolase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Amidohydro_1" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_006123619.1" /locus_tag="EAO74_RS31790" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_31825" /product="amidohydrolase family protein" /protein_id="WP_261988423.1" /sec_met_domain="Amidohydro_1 (E-value: 1.5e-22, bitscore: 77.8, seeds: 57, tool: rule-based-clusters)" /transl_table=11 /translation="MSDHEAAPVLRIKGRVLVGPDEVRDGLWAVDGRITYERPPGADDA VTLTGWVLPGLVDAHCHVGLDHQGPVDAATSEKQALTDREAGTLLIRDAGSPSDTRWTD AREDLPKIIRAGRHIARTRRYIRNFAHEIEPGDLVAYVAQEARRGDGWVKLVGDWIDRD AGDLTACWPRGEVEAAIAEAHRLGARVTAHCFAEDALRDLVEAGIDCIEHATGLTEDTV PLFAERGVAIVPTLVNIATFPGLAAAGEARYPRWSAHMRRLHETRYETVRAAYDAGIPV FTGTDAGGSLAHGLIADEVAELVKAGIPPLDALSATTWGARRWLGRPVLEEGAPADLVM YDEDPRADVRVLASPRAVVLNGRVRG" misc_feature 10161..10176 /note="TFBS match to CopR, Copper-responsive regulator, confidence: weak, score: 16.99" /tool="antismash" misc_feature 10172..10185 /note="TFBS match to PsrA, Oleic acid responsive regulator, confidence: weak, score: 18.44" /tool="antismash" ORIGIN 1 cgcagctcgc cggcctcctc accgccgggg cggacgcggc gaaggcggtc tcctccacga 61 cccaggtggc tctgcacctc gccgagggcg gcgacaacgc gggcacccgc tggtggttcg 121 acaacgcggt ggcgcagaag gtgcccttcg acgtcatcgc cctctcgtac tacgcctact 181 ggcacggctc cctggccgag ctccagacca acctggacga cgccgcctcg cgctacggca 241 agccggtcat ggtcgcggag acggcgtacg cccgtacgct cgcgaacgac gacggcctgg 301 agaacaacgt ggccaccgcg agccagctgg tcaccggcta cccggcgacc ccggccggcc 361 aggcggcgaa ccttcgcgac gtcatgaacg tcgtggaggc cgtgccgaac ggccggggcc 421 tgggcgccgt ctactgggaa ccggcctgga ccgccgtcgc cggcagcggc tgggacccgg 481 ccgacccggc gtcgggcaac gcgtgggaga accaggccgt gttcgaccac gacggcaggc 541 tgctgccggc ggccggctgg ttctcgcacc ggtagaccgg cgggccgttc cgacggcccg 601 gagtgtgccc cgtccgggcc gtcgcacctc tcagacgggg cgcgcgccct cgatgttggc 661 caccatcgtc cggagcacct tcagcgcggc cacgtactcc gcgtcgctga tcccccggtg 721 gacctcggcg cggagaccgg tcaccagctc acgcagccgc gcccgggcgg cctccccggc 781 gtcggtgagg ccgaaccgct gcccgtcccg gctgatccat ccccggtgga gcagctggtc 841 gacggccctg gagatctcct gcggcccgtc ggccaggggc gtgagctcgg cgaccagctc 901 ctcacgggtg ggcgcggcga gtccgtagcg cacccggttg agcacccagt actggggctg 961 cgtgacatcg accccggcca gggcgtcccg cagatgccgg gtgacggcgg agtgggccag 1021 gccgctccag tacccgacgg gctgtgtggc cagggcgtcg tccgccgccg ccgggtccgc 1081 gggcgcctga tcggtgaggg tggtgctggt cggctgctcc atggccgtga cgctaccggc 1141 gcgccccggc cggcggcccc ggaggcaccg gaaccggccg gaacgcggcc ggttccggcg 1201 gctcagggct ccggcggccg ggcgatccgg tgactcaccc ctccagcggc gcgccgtgcc 1261 acctccacga ggcgacgcgg gcgcgccccg gcagatccgc gcggccggtg gaccacagca 1321 ggacggtcca ccggtcctcg tcgtccgggg cgtcggggaa cagccgggcc agcacgcggt 1381 cgcagaggtc ccggggcggg gtccaggaga gggacagccc ggcggcgacg tcatgggtgt 1441 ggaccagggt ctcgacgatg cccatcgcgg cgaagccctc cgggtcggag acgccgtgcg 1501 agtggtacga acggaggtcg ggggaggccg tccgcaccat cgctgtcagc agcgcgccgc 1561 tcgcttccag cgtctggagc aggccggcgg tccccgcccc ggggtccgcg aagaccgagt 1621 tcgaggggcc gccctcgcgg tccggggccc agcggtaggg gacctcggtc tccagggagg 1681 gcctgcgcgg gccgagctgg acggcgtacg cgaagaggtc gtcgctcagg tgctccacgg 1741 tctcccagca gtcccacgtc aacgacccgg ccgggatatg ccagtcgtcc accggggacg 1801 ccgcgagggt gtcgaccgcg agccgtaccg ccagcgtcac gtcgtcggcg gtgaccggca 1861 gccgtgtgcc ggtcagcgcc gcgcgctcgg ctgccccgca ctccacgcag aactcgttgc 1921 cctccggatc ggccagcgtc gcccagcccc ggccgttcgg cttgcggtgg tccgcgacca 1981 gcgtggcgcc cagggcgagc agccgctcga cctcctcgtc gcgggtacgc cgctcgggac 2041 ggatgtccag atggatccgg ttcttgccgc tcttgggttc cggcacggtg atgaagagca 2101 gcgcggcgcc cggcgcctcc acgagcgcct cgggatcgcc gggcgcgtcg tcgtcggaga 2161 cctgcgagcc gagtacctcg gcccagaaga gggccagttc gtaggcgtcg gcacagtcga 2221 tggtgatgtg ctgtacgagc gaagtcatgg acgccactgt ctccgctgat ccgtgccggg 2281 tccagcgagt tgtacggtcc ggtggcccta cgggcgggtc acggccgccc gcaccaggga 2341 ccgcgccagc tcgacgctgc ccgggagggt gtcgagcggg aggggctgcg tggcgaggac 2401 gccctcgtgc agcacgagga gctgggtggc cagggcgccg ggggccgagc agcccgcagc 2461 ggtggcgagc tcctcgaaca ggtcgacgag ccagcgcttc tggtccgcgg cgatgacgta 2521 cgcgggatgg gaggcgtccg ggagctcggc cagggcgttg atgaacgcgc atccccgggc 2581 gttggtcccg ctccagaccc gcagtgcctc gaagggggcg gtgacggcct cgaccgggtc 2641 gtcgcaggcg tcgacggtgg cgcggacgag cgactgccag cgccggtcgc gtgccgcgag 2701 gtaggcggcc acgagccggt ccttggagcc gaactggttg tacagggtcc gcttggtcac 2761 ccccgagtgc tcggcgatca gatccacgcc gaccgccgtg atgccgcggt cgtagaacag 2821 ctcctcggcg gccgcgacga cgcgctcgcc ggccggcgtc agggggcgtg tgtcctgcat 2881 cagcattcct tcaccgatcg gtgtaccgtg gagtgtgttc acagatcagt atacctagca 2941 ggaggtcgag tgatggacgt gcccggaacc atgcgcgcag tgcggatcgc ggagcacggg 3001 gggcccgagg tcctccagcc gtcagaggtc gccgtgcccg cgccgcgggc gggagaggtg 3061 ctggtcaggg tcgccgcggt ggcgctgaac aacaccgacc tgtggacccg tgagggcgcc 3121 tacggccggc ccggagaccc ggaggcgctg tccggctggc gaggcccgct cgacttcccg 3181 cgcatccagg gcgccgacgt ggccggccgg gtcgtggccg tcggttccgg cgcggacggg 3241 agcctcctcg gccgccgggt ggtcgtcgac ccggcgatct acgactcgga aggaccggac 3301 gcgaacccgg tgggcctgat ggggagcgaa cgcgacggcg gttacgccga gtacgtcacc 3361 gcgcccgccg ggcgtgtgca cgacgtgacg gactccccgc tcaccgacga ccagctcgcg 3421 acgctgccga ccgcctacgg cacggcgctg ggcatgatcg agcggggcag gctgggcaag 3481 ggcgagaccg cgctggtctc gggggcgtcc gggggagtgg gcctcgcgct gatccagatc 3541 gcccgggccc gcggtgcgaa ggtgctcgcc atcagcagcg gatccaagac cgacgcggtg 3601 cgtgaggcgg gcgcccacga ggtcctcgac cgggcgcagg acgtcgccga gcagatccgc 3661 gccgtcgccc cggagggcgt ggacgtcgca ctggacgtcg tcgccggtga cctggtgggc 3721 tcggtgctgc cgctgctgcg cgagggcggc cgatgggtcg tcgccggtgc gctcggaggg 3781 tacgaggtgg ccttcgacgt acgccggctc tatctgcaca acgcgcaggt gatcggctcc 3841 gcgatgcaca cgcccacgca gttcggcctc ctcatggacc tggcccggca gggcacggtc 3901 cagcccgtca tcgccgcgac ctaccccctg gagcaggccg ccctggcaca ggaggcgctc 3961 tcccgcaggg gccacgtggg aaagatcgtc atgcacccgt gatcgtcatg cacccatgag 4021 gggagaacgt cccttgcgcg gcacaggacg aggacggggc gggacgcgtg atgcgtcccg 4081 ccccgtcctc gtgccgtacg tccctacttc accggcgtga agtcacgcgc cccgatgaag 4141 tcgggccggc ggatcggagc cgcgaagggc tcctgcgccg tgttctccac gctgttgaag 4201 acgatgaaga cgttgctgcg cgcgtacggg gtgatgttgt cccccgagcc gtgcatgcag 4261 ttgcagtcga accaggtcgc cgaaccggcc gcgcccgtga agagcttgat gccgtgccgg 4321 tcggccatct tcgtcagcgc ctcgtcggac ggggtgccgg cgtcctgcat ctgcagggac 4381 ttcttgtagt tgtccttcgg cgtctcgccc gcacagccga ggaacgactt gtgcgaaccg 4441 ggcatgatca tcagcccgcc gttggtgtcg aagttctcgg tcagcgcgat cgagaccgac 4501 acggtccgca tgttcggcag accgtcctcg gcgtgccagg tctcgaagtc cgagtgccag 4561 tagaagcccg aggcaccgaa gcccggcttg acgttgatcc ttgactggtg gacgtacacg 4621 tcggagccga ggatctggcg ggcacggccc accacacgct cgtcgcggac caggttcgcg 4681 aagacctggc tgagcttgtg gacctcgaag acggaccgca cgctctgcga ctgcggttcg 4741 atgatcgagc gctcgtcggc ccggacctgc gggtcggcga ccagccggtc cagctcggcc 4801 cggtagacgt ccacctcgtc cggagtgatg agctggtcga cggtgaggaa gccgttctcc 4861 tcgaagccct ggagatcctt ggcggtgacc ggcccgggcg cgcccggcgc ggaccagatg 4921 accgggccct ggcggggagt ggtcatctcg gcggcgccgc gcgaggggta caggtcggcg 4981 cgtacatcgg tggtcatggt tcagccctcc tctgtcagca gcgggtatac accgttctcg 5041 tcatggtcct cccgcccggt gacgggggga ttgaagacgc acacgcagcg gaagtcggtc 5101 ttcggccgca gcgtgtggtg ctcgtgcccg ttcagcaggt acatcgtccc gggggagatc 5161 cagtgcttct cgccggtctc gtcgttggtg agctcggcct cgccctcggt gcacagaacg 5221 gcctcgatgt ggttcgcgta ccacatcgac gtctccgtgc ccgcgtacag cacggtctcg 5281 tggagagaga agcccacctt ctccttggcg agcacgatgc gcttgctctc ccaggtacct 5341 gatgcggact tgatgtgccg gtcggtgttc tcgatgtcac tgaaagatcg gacgatcacg 5401 gtggggtggt gcctttctct tctgtcctgc tctgtggtgc ctacgcgggg gagtgccccg 5461 ctcaggcggt ctctcggacg gagcgggcca gcgtgcgcag gccctcctcc agctcatcgg 5521 gggtgacggt cagcggcggg agcagcttca cgacctcgga ctgcgggccg gaggtctcca 5581 gcagcaggcc cagctcgaag gcgcgcttgc agacggcgga cgcgcgctcg gggtcggtga 5641 actccaggcc ccagaccagg ccgcggccgc ggaactgcgc ggtgtcgtcc tcgccgcaga 5701 tcgccagcag cgtctgctcg acctgctcgc cgcgggccag ggtctgcttc tccatctggc 5761 cgtcggccca gtaggcgtcg agcgcggcgg cggcggtgac gaacgccggg ttgttgccgc 5821 ggaaggtgcc gttgtgctcg cccggctccc agatgtccag ctcgcccttg aagaggcaga 5881 gcgacatcgg caggccgtag ccgctgatgg acttcgacag cgtcacgatg tccgggacga 5941 tgccggcctc ctcgaaggag aagaagccgc cggtccggcc gcatcccatc tggatgtcgt 6001 cgacgatcag gagcatgtcc tggcggtggc agaggtcctg gagggcgcgc agccactcgg 6061 cgcgggcgac gttgatcccg ccctcgccct gcacggtctc cacgatcacg gcggcgggct 6121 tgttcagccc ggaaccctgg tcctccagga gccgctcgaa ccagaggaag tcgggcacct 6181 ggccgtcgaa gtagttgtcg aacggcatcg gggtgccgtg gaccagcggg atgccggcgc 6241 cggcccgctt gaaggcgttg ccggtcacgg cgagcgagcc gagcgacatg ccgtggaagg 6301 cgttggtgaa cgagacgacg gactcgcggc ccttgacctt gcgggcgagc ttcagcgccg 6361 actcgacggc gttggtgccc gtcgggccgg ggaacatcac cttgtagggc aggtcacgcg 6421 ggcgcaggat cacgttctcg aaggtctcga ggaacgcccg cttggccgtc gtggccatgt 6481 ccaggccgtg ggtgatgccg tcgcgctcga tgtagtcgat gagcgcgcgt ttcagcaccg 6541 ggttgttgtg cccgtagttg agagatccgg caccggcgaa gaagtcgagg tacgagtggc 6601 cgtcctcgtc ggtcaggcgg gcgccctgcg cgcggtcgaa gaccgcgggc caactgcggc 6661 agtagctccg tacttccgac tccagggctt cgaagacgct cagggcgggc ggggtgatgg 6721 tcacagctga tctcctgctc gaggggggtg tgacgggcgg cgggtcaggc atgcccggga 6781 aagccgcgcc cgggcggggc gcggtagggc gctgggaggc cctagaacgg gccgatgcgg 6841 tagagaacct ccggcaggtg cgtcccctcg gggaacagcg ccccctcgaa gagcacctcg 6901 tgctccagcg gcgcgtcgtg ccgctgcgcg aaggacgtga acagccggtc ggaggcggcg 6961 ttgtccgggg tgatcgtcgt ctcaaccgat gtcagaccct ggtcggaggc gacacgggcg 7021 gtcagtgcgt ccagcagctt ggccgccagt cccttgccac ggtgggcccg gtcgacggcc 7081 acctgccaga cgacgagcgt ctcggggcgg tcggggcgga tgtatccggt cacgaaggcg 7141 atggggtcgc cggtctcgtc gcgggcgacc acggaggtcg ccgcgaagtc acggcaccac 7201 agcaggtagc tgtacgagga gttgaggtcc aggacctcgg agtcgcgggc aatgcgccag 7261 atcgcggctc cgtcctccac tcgcggggtg tcgattctga ggaattcgct tcgggcactt 7321 gcaaaatctg ctggtgcggc ggtcatgcga attgaattta cccagcaaaa atcaaaattg 7381 catcgccgca gggggttggg tcggcgcgct cgttgtgtta tcacgcgggc gcggatccac 7441 ctcgatcgag gggtgtttta gccggtcctg tccggaattt aacgcccaaa gcggactcga 7501 tgtggggtcg gtcacatcgt tgtaactctc ctgacatccg tccggatcgg ctgggccgat 7561 cgagtgaaaa ctgtggcgtt tagcctctga gaacggggga agaagaatgc ggggagctgc 7621 tcccgataaa gcagctcccc ttttcgtgtc cgcgtccgat aaaccggccg ctgtagttcg 7681 catttaattc agttcatcgg ccaggttttc gcgacggctt cgcgggcagc atcgaggtcg 7741 gtctcccgtc ccgcgtccgc cagtgcggcg cccagggcgg ccagcgagga cagcaccgcg 7801 tcccgcgtcg cgtccgcgcc gtagtggttg acccggatca tctccctgga cagcgcgccg 7861 ccacccgcga tcagcggcag cgagggcccg gtcgcgagcg cccggccgac cagctccgcc 7921 gcgtcgagat ccgccggtac gcgcagggtc gtggcgacgg gcgccgcgtc ccgggcgtca 7981 tggacgtacg gctccagccc gccgccgagc gccgaggcgc ccgcccgggt cgccgcggcg 8041 gccgacgcgt gccgcgcgat gaccgtgtcc aggccgtccg cctcgatccg gtccaggcag 8101 gcgtcgagcg ccagcatctc cagctgggcg ggcgcgtgcg gcagcgccct gcggcccccg 8161 tcgatccagc gggccttcca gtccagcagc gagaggtacg actgccgcgg agcccccggg 8221 ttggcggcca tccgctccca cgcccgcgtg ctcaccgaca cggcggagac cccggccggg 8281 ccgcccatgg ccttctgcgc gccgatgacg cacaggtcca cgccccagtc gtccaccagc 8341 agcggctccg cacccaccga cgcgacggcg tccaccatga acagcgcgcc gtgtgcccgg 8401 accacctcgc cgatctcacc gaccgggttg gtgttgccgg tcgccgcctc ggcgtgcacg 8461 agcgacacga agtcgatctc cgggtgctcg gccagtgcct gccggacctg ctcggcggtg 8521 accgcggtgt ggaacggcac cgccaggtcc acgacctcgg cgccgcagtc gcgcagccag 8581 ttgccgaagg tctggccgta cggcccggtc acgacgttca gcgcggtcga gccgggccgg 8641 gcgccgcccc ggatgcagcc ttcgaggggc agcagcgcct cgccctgcat gatgacgacg 8701 tcctgctcgg tggcgaggag gccggccacc cgccgctcga tcgctgcgaa acgcgcggcg 8761 gtgagcgggg cgaggtcgag gaatgggtgt gtcacggtgc tgctctcttc gggtcggccg 8821 gtgcgctgtc agcgcctgcg cgtcgagggt acccagcacc ctccctggcc cccgcggcct 8881 cctcgtacag tgctgtccat gagcgatcac gaggcggcgc cggtgctgcg gatcaagggg 8941 cgggtgctcg tcgggcccga cgaggtgagg gacggcctct gggcggtcga cgggcggatc 9001 acctacgagc ggccgccggg cgcggacgac gccgtgacgc tcaccggctg ggtgctgccc 9061 ggtctggtcg acgcgcactg ccatgtgggg ctcgaccacc aggggcccgt cgacgcggcc 9121 accagcgaga agcaggccct caccgaccgt gaggccggca ccctgctcat ccgggacgcc 9181 gggtcaccct ccgacacccg gtggacggac gcgcgcgagg acctgccgaa gatcatcagg 9241 gcggggcgtc acatcgccag gacgcgccgt tacatccgca acttcgccca tgagatcgag 9301 cccggtgacc tggtcgccta cgtcgcccag gaggcccggc gcggcgacgg ctgggtcaag 9361 ctggtcggcg actggatcga ccgggacgcc ggcgacctga ccgcctgctg gccgcgcggc 9421 gaggtcgagg ccgcgatcgc cgaggcccac cggctcggcg cccgggtcac cgcccactgc 9481 ttcgccgagg acgcgctgcg cgacctggtg gaggccggga tcgactgcat cgagcacgcg 9541 acgggcctca ccgaggacac cgtcccgctc ttcgcggagc gcggtgtcgc gatcgtcccc 9601 accctggtca acatcgccac cttccccgga ctcgcggcgg ccggcgaggc caggtacccc 9661 cgctggtccg cccatatgcg gcggctccac gagacccgct acgagaccgt gcgcgccgcc 9721 tacgacgcgg gcatcccggt cttcaccggc accgacgcgg gcggctcgct ggcccacggc 9781 ctgatcgcgg acgaggtggc cgaactggtg aaggccggca tcccgcccct ggacgcgctc 9841 tccgcgacca cctggggcgc ccggcggtgg ctgggacggc cggtgctgga ggagggcgcc 9901 ccggcggacc tggtgatgta cgacgaggac ccgagggcgg acgtacgggt gctggcgtcc 9961 ccccgcgcgg tggtgctcaa tgggcgggtg aggggctgat cgccggttga cccgctcagg 10021 ggcgccgtgt tgaccggcgg tgaccccggg cgccgcgtct cgttgaacca cctctgtgct 10081 cggcctgggg catcccgtga tcatgccctc gaagcagtga aacgggtggt tccgaggaac 10141 tcctgtgcca gtggattcga atgtgcacac ggaaacccct ctttggagtg aactggcgga 10201 aggtgtctgc cagttcaccc tctgtgcgta aagattcacg gagtcgaggc caccggtgcc 10261 cgcgatgtcc cccattggac ggctccggtg gctccatgtc tcttgtgggg gttccaccat 10321 cttgaacagc aacaccttcc gttcggctgc cctggcgatc gccgccgctc ccgtcgccct 10381 gctcgtcgcc gtccccgcg //