LOCUS NZ_RDBM01000035 10204 bp DNA linear CON 01-JAN-2025 DEFINITION Streptomyces sp. gb1(2016) Scaffold_35, whole genome shotgun sequence. ACCESSION NZ_RDBM01000035 VERSION NZ_RDBM01000035.1 KEYWORDS WGS; RefSeq. SOURCE Streptomyces sp. gb1(2016) ORGANISM Streptomyces sp. gb1(2016) Bacteria; Bacillati; Actinomycetota; Actinomycetes; Kitasatosporales; Streptomycetaceae; Streptomyces. REFERENCE 1 (bases 1 to 2333361) AUTHORS Hariharan,J., Choudoir,M.J., Diebold,P., Panke-Buisse,K., Campbell,A.N. and Buckley,D.H. TITLE Direct Submission JOURNAL Submitted (16-OCT-2018) Soil and Crop Sciences, Cornell University, 306 Tower Road, Ithaca, NY 14850, USA REFERENCE 2 (bases 1 to 2333361) AUTHORS Hariharan,J., Choudoir,M.J., Diebold,P., Panke-Buisse,K., Campbell,A.N. and Buckley,D.H. TITLE Direct Submission JOURNAL Submitted (16-OCT-2018) Soil and Crop Sciences, Cornell University, 306 Tower Road, Ithaca, NY 14850, USA COMMENT ##Genome-Assembly-Data-START## Assembly Date :: 2018 Assembly Method :: A5+MeDuSa v. JUNE-2018 Genome Representation :: Full Expected Final Version :: Yes Genome Coverage :: 66x Sequencing Technology :: Illumina MiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_008042065.1-RS_2025_01_01 Annotation Date :: 01/01/2025 02:23:01 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.9 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 7,414 CDSs (total) :: 7,333 Genes (coding) :: 6,887 CDSs (with protein) :: 6,887 Genes (RNA) :: 81 rRNAs :: 6, 1, 2 (5S, 16S, 23S) complete rRNAs :: 6, 1, 1 (5S, 16S, 23S) partial rRNAs :: 1 (23S) tRNAs :: 69 ncRNAs :: 3 Pseudo Genes (total) :: 447 CDSs (without protein) :: 446 Pseudo Genes (ambiguous residues) :: 0 of 447 Pseudo Genes (frameshifted) :: 94 of 447 Pseudo Genes (incomplete) :: 395 of 447 Pseudo Genes (internal stop) :: 7 of 447 Pseudo Genes (multiple problems) :: 48 of 447 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-13 13:17:57 NOTE :: This is a single region extracted from a larger record! Orig. start :: 787880 Orig. end :: 798084 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to RDBM01000035.1. Annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (released 2013). Information about the Pipeline can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ FEATURES Location/Qualifiers region 1..10204 /candidate_cluster_numbers="1" /contig_edge="False" /product="RiPP-like" /region_number="2" /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /tool="antismash" cand_cluster 1..10204 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /kind="single" /product="RiPP-like" /protoclusters="1" /tool="antismash" protocluster 1..10204 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="False" /core_location="[5000:5204](-)" /cutoff="20000" /detection_rule="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /neighbourhood="5000" /product="RiPP-like" /protocluster_number="1" /tool="antismash" proto_core complement(5001..5204) /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /neighbourhood="5000" /product="RiPP-like" /protocluster_number="1" misc_feature complement(6..20) /note="TFBS match to VqsM, Regulator of quorum-sensing signalling systems, confidence: medium, score: 18.37" /tool="antismash" gene complement(259..2796) /locus_tag="EAO74_RS14090" /old_locus_tag="EAO74_14100" CDS complement(259..2796) /GO_component="GO:0016020 - membrane [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016828175.1" /locus_tag="EAO74_RS14090" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_14100" /product="FtsX-like permease family protein" /protein_id="WP_187281817.1" /transl_table=11 /translation="MMRPNGLARAAVRFKPASFAGTFLALLMSALIVAACGILLETGLR ASVPAERYAKVPVVAAADQYVRVVTGSGEDREEEAVPLPDRARMDAGLAAKAATAPGAA TAVADFTFPVRTADATPGSRELPVPGGALTAHGWGSHAFTGTALTTGSAPHDGEVVLDA GTARAAKAAVGDTVTLRTAAGRRDFRVAGVAEAGPGDTARNTGAGDALAWFADTQAPAL AGHPGRADAIAVLAEPGTRAGALAGSVRTALAGSGAGVHLGDDRGAVEDPGLGYAKETL FGIGGSFGGIATIVAIFTAAGTVALSVSQRAREFALLRAVGATPRQIRRAVASEALLVA PLAGIAGCLPGIGLAHWWFGQLQDRGAIPEAVRVHVSWIPLLVAVATGLLTALAAGWAA GRRPARTKPGQALTEASVERLRPGVVRTVLGVAALVGGAILTGVSANSAGDDAAGASLG VVMCFMLAVALLGPLVARVCAGLFGLPLRGAGPAGELAAANSRTNARRLASAITPIVLA MAFASTLVFMQTSESHIADKQLRAGLTADHVVTDPAGIPVDAAARAARAPGVEAVVGVL STQVLIPVGSGEFAALQGAAVQGVTGSGEDLAKVQDLDVREGSLDRLGAGRIAIDTTLA TSAGVGVGDPLPLHLPDGTKARPEVVAVYGHGLGLATVTMDRASLAGHVTSGFDSTLLV RGGSEESLAGFGEVTDASGYATGRSLDAELGAWSNTTMAAVLGGFAAVAAVNTLVMTVL DRRRELGTLRLVGSTRRQVLQMLHWEGLLVSAVGVTLGTAIAAATLIPMIRGLTGDGPY VPPLVYGSFVAAAVALVLLAVTLPARAALRRWS" gene complement(2793..3878) /locus_tag="EAO74_RS38535" /old_locus_tag="EAO74_14105" CDS complement(2793..3878) /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /codon_start=1 /gene_functions="transport (smcogs) SMCOG1288: ABC transporter related protein" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_003975187.1" /locus_tag="EAO74_RS38535" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_14105" /product="ATP-binding cassette domain-containing protein" /protein_id="WP_147983744.1" /transl_table=11 /translation="MDTSVTTRGTAPATPVTGAAASLRKLYFLRFAFAAVWAGLLFATA DTLGPLSAALLVVYPLFDVACAVVDARSARAVGGPVRGLYVNIALSTLTAVGLAVAATS GIPAVLRVWGIWAVTAGLVQLVVGAVRRRLGGQWAMIVSGAISTLAGASFFAQAGADGA TLSNLAGYAFLGGVFFLVSALRLKRPGEGGDGTADDHRPLAAQPASYGAYDELDEARGD GPDAPDPQDRGDAGRGGDGEAYGGEGAGELSGGQQQRVAVARALVTAPDVVFADEPTGA LDTGTAAEVLGLLRQAVDQLGATVVMVTHDPVAAAWADRVLFLADGAFADGVERGSAEL IAERMAALTARTARVGAGVAA" misc_feature complement(3924..3937) /note="TFBS match to LexA_variant_2, Repressor of DNA damage response, confidence: weak, score: 18.69" /tool="antismash" misc_feature 3975..3988 /note="TFBS match to LexA_variant_2, Repressor of DNA damage response, confidence: weak, score: 18.69" /tool="antismash" gene 4017..4622 /locus_tag="EAO74_RS14100" /old_locus_tag="EAO74_14110" CDS 4017..4622 /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_function="GO:0003700 - DNA-binding transcription factor activity [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1057: TetR family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014048110.1" /locus_tag="EAO74_RS14100" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_14110" /product="TetR/AcrR family transcriptional regulator" /protein_id="WP_147983745.1" /transl_table=11 /translation="MSRGTGSGSTSEARARLLGTATRIFYAEGIHSVGVDRIIAEAQVT RATLYRHFSGKEELVLAYLDQADRGIRAQVASAQAEDAPAAERIRAVSRSITEGIRSPG FRGCAFLNAVAEYPDPAHPIHQAVLAHRQWFLDTVTELLAEAGHTPAGAAGRHLVMLRD GAMAEGCLFDPELVSETFLQAVEELLRARPASTGRVTA" gene complement(5001..5204) /locus_tag="EAO74_RS14105" /old_locus_tag="EAO74_14115" CDS complement(5001..5204) /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) RiPP-like: strepbact" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_014153869.1" /locus_tag="EAO74_RS14105" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_14115" /product="hypothetical protein" /protein_id="WP_147983746.1" /sec_met_domain="strepbact (E-value: 2.6e-15, bitscore: 53.7, seeds: 15, tool: rule-based-clusters)" /transl_table=11 /translation="MSSAPQIQAQEISDADLDNVSGGTFAGLAGEVALETPLSSISAGF SGALGVDGLGVAGLGSTSTLSA" misc_feature 5274..5286 /note="TFBS match to MatP, Cell cycle regulator, confidence: weak, score: 19.5" /tool="antismash" gene complement(5702..7594) /locus_tag="EAO74_RS14110" /old_locus_tag="EAO74_14120" CDS complement(5702..7594) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018518577.1" /locus_tag="EAO74_RS14110" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="EAO74_14120" /product="SMI1/KNR4 family protein" /protein_id="WP_147983747.1" /transl_table=11 /translation="MSDETFDWRDFLGRWQEEWVPGEDDEQDDEPPLAGPARPGADEAA IAAAERRLGQRLPPSYREFLAASDGWRVEQTAAIYELGGAADIDWFHDPHGMTPLYEEG LGDDPREEEVLLAGMWQRALRLETESDMSSALLDPGDRDQDGEWALYVYKGWSGEYPDR YPSFRAYMEAMYRGFHGDRAGMPGFVNATTRAQDAHVEEARLLALRGRHEEALPLLEDA LSFGRPHSATLLNQLRHLTAPQTARDYGFLVADPSCLPEILPLQAMEPARGERGPDGDA HWLGMMAARGVPRETAEAVLGAVRDGTHRYAPPGPWGRAVAEAREAARWGATDAAWRVL RAALPLWEAPGPQLIAPVGLLADPFLGPLVTPERGREILATPRAGEQGPAPDPVPDLDP PGLAWLTESDPYRQADDGYRCVWAEGIDPARLPGLIGEEGSTLSAAMDQRWGRWRRASV AETQEHAEPWEDRAPVAVGRTAGGWAFAFDRDPHHLGERFVSPAAAASASGRAVVVWHE PGRPSPGDRPPTFHLSVAEQGEELYAFTVRGTEIQRSGAIPDALDPAGLFLPDATERDN ELRALAALHTKLGLSLPRFALTRGRLSTFTTRSWTRAPREGESYAYIRFVRHRS" tRNA complement(7649..7722) /anticodon=(pos:complement(795567..795569),aa:Trp,seq:cca) /inference="COORDINATES: profile:tRNAscan-SE:2.0.12" /locus_tag="EAO74_RS14115" /note="Derived by automated computational analysis using gene prediction method: tRNAscan-SE." /old_locus_tag="EAO74_14125" /product="tRNA-Trp" gene complement(7649..7722) /locus_tag="EAO74_RS14115" /old_locus_tag="EAO74_14125" misc_feature 7742..7755 /note="TFBS match to PsrA, Oleic acid responsive regulator, confidence: weak, score: 17.39" /tool="antismash" misc_feature complement(7951..7964) /note="TFBS match to BldD, Development and antibiotic global regulator, confidence: weak, score: 19.14" /tool="antismash" ORIGIN 1 gatgatggcc gatctcgacc gctacctctc ccgcgacgac gccgatcccc cgaacgacgg 61 cgtcctctgc cggcagggag cggtatgggt gacccccggg gagttcaccg aactggtggc 121 ggaactggag gccctggtcg cccggtacgc ggggcgcgcc cccggcgacg accgcatccg 181 tcacatcgtc agtctcgccc tggtgcccga cagggcggcc ggggaggagg gccgggcatc 241 cgaggcgtag ggcacgggct acgaccagcg gcgcagcgcg gcccgtgcgg gcagggtcac 301 tgcgagcagg accagggcga cggcggccgc cacgaaggag ccgtacacca gcggcggcac 361 gtatgggccg tcgccggtca ggccgcggat catcgggatc agcgtggccg cggcgatggc 421 ggtgccgagg gtcacgccca ccgcggagac cagcaggccc tcccagtgga gcatctgcag 481 cacctggcgc cgggtggagc cgaccaggcg cagcgtgccc agctcgcgcc ggcggtcgag 541 gaccgtcatc accagggtgt tgacggcggc gacggcggcg aagccgccga ggaccgcggc 601 catggtggtg ttggaccagg cacccagctc cgcgtcgagg ctccgcccgg tggcgtagcc 661 ggaggcgtcg gtgacctcgc cgaagccggc gagcgactcc tccgaaccgc cccgtaccag 721 cagcgtgctg tcgaagcccg aggtcacatg cccggccagg gacgcccggt ccatggtcac 781 cgtggccagg ccgaggccgt ggccgtagac cgcgacgacc tcggggcggg ccttcgtgcc 841 gtccgggagg tggagcggca gcggatcgcc gacgccgacg cccgccgagg tggccagcgt 901 ggtgtcgatg gcgatgcggc cggcaccgag ccggtcgagg ctgccttcgc gtacgtccag 961 gtcctgcacc ttcgcgaggt cctcgcccga tccggtgaca ccctggaccg ccgcgccctg 1021 gagcgccgcg aactcgccgg agccgaccgg gatcagcacc tgcgtgctga gcacgccgac 1081 aaccgcctcc acccccggcg cacgggccgc tcgggccgcg gcgtccaccg ggatccccgc 1141 cgggtccgtg accacgtggt ccgcggtgag gcccgcgcgc agctgtttgt cggcgatgtg 1201 gctctcgctc gtttgcatga agacgagcgt cgaggcgaag gccatggcga gcacgatcgg 1261 ggtgatcgcg gaggctagac ggcgggcgtt cgtacgggag ttggcggccg ccagttcacc 1321 ggccgggccc gctccgcgca gcgggaggcc gaagaggccc gcgcacaccc gggccaccag 1381 cgggccgagc agggcgacgg cgagcatgaa gcacatgacg acgccgaggg aggccccggc 1441 cgcgtcgtcc cccgcggagt tcgcggagac gccggtgagg atcgcgccgc cgacgagggc 1501 ggcgacgccc agcaccgtac ggacgacgcc cggacgcagc cgctccacgg acgcctcggt 1561 gagcgcctgc cccggcttgg tcctcgcggg cctgcgcccg gcggcccagc cggcggcgag 1621 cgccgtgagc agcccggtgg cgacggcgac gaggagcggg atccaggaga cgtgcacccg 1681 taccgcctcg gggatcgcgc cccggtcctg caactgcccg aaccaccagt gcgccagccc 1741 gattccgggc agacagccgg cgatcccggc gagcggcgcg acgagcagcg cctcggaggc 1801 gaccgcgcgg cggatctgcc ggggggtggc tccgacggcg cgcagcagcg cgaactcgcg 1861 ggcccgctgg gacaccgaca gggccaccgt cccggccgcg gtgaagatcg cgacgatggt 1921 ggcgatgccg ccgaaggagc cgccgatccc gaagagcgtc tccttcgcgt agccgagacc 1981 ggggtcctcg acggcgcccc ggtcgtcgcc gaggtgtacg ccggcgccgg agccggccag 2041 ggccgtccgc acggatccgg cgagggcgcc ggcgcgcgta ccgggctcgg ccagtacggc 2101 gatggcgtcc gccctgccgg ggtggccggc gagcgccggg gcctgggtgt cggcgaacca 2161 cgcgagggcg tccccggcgc cggtgttccg ggccgtgtcc ccgggtccgg cctcggcgac 2221 gcccgcgacc cggaagtccc gtcgcccggc tgcggtccgg agggtgacgg tgtcgccgac 2281 ggcggccttc gcggcgcgag ccgtacccgc gtcgagcacg acctcgccgt cgtggggcgc 2341 ggaacccgtg gtcagcgccg taccggtgaa ggcgtgcgag ccccacccgt gggcggtgag 2401 cgcaccgccg ggaacgggga gttcacgcga gcccggcgtg gcatccgccg tgcgcacggg 2461 gaaggtgaag tccgctacgg cggtggccgc cccgggtgcc gtggccgcct tcgccgccag 2521 tccggcgtcc atccgtgccc tgtccggcag cgggacggcc tcctcctcgc ggtcctcgcc 2581 gctgcccgtt accacgcgta cgtactggtc ggccgccgcg acgaccggca ccttcgcgta 2641 ccgctccgcc ggcaccgacg cccgcaggcc cgtctccagc aggatgccgc aggccgcgac 2701 gatcagcgcg gacatcagca gcgcgaggaa cgtcccggcg aaggacgcgg gcttgaagcg 2761 gacggcggcg cgcgccagtc cgttggggcg catcatgccg ccaccccggc ccccacacgg 2821 gcggtacggg ccgtgagcgc cgccatccgc tccgcgatca gctccgcaga accgcgctcc 2881 acgccgtccg cgaaggcgcc gtccgcgagg aagagcaccc ggtccgccca ggcggccgcc 2941 accggatcgt gggtgaccat gacgaccgtc gcgcccagct ggtcgacggc ctgccggagc 3001 aggccgagga cctcggcggc ggtgccggtg tcgagggcgc cggtgggctc gtcggcgaac 3061 accacgtccg gggcggtgac cagcgcgcgg gcgacggcca cccgttgctg ctggccgccc 3121 gacagctcgc cggcaccctc accgccgtag gcctcgccgt cgccgccacg tccggcatcc 3181 ccgcggtcct gcgggtctgg ggcatctggg ccgtcaccgc gggcctcgtc cagctcgtcg 3241 taggcgccgt acgacgccgg ctgggcggcc agtgggcgat gatcgtcagc ggtgccatct 3301 ccaccctcgc cggggcgctt gaggcggagg gcggagacca ggaagaagac gccgccgagg 3361 aaggcgtaac cggccagatt gctcagcgtg gcgccgtccg cgcccgcctg cgcgaagaag 3421 gacgccccgg cgagggtgga gatggcaccg ctgacgatca tcgcccactg gccgcccagc 3481 cggcgtcgta cggcgcctac gacgagctgg acgaggcccg cggtgacggc ccagatgccc 3541 cagacccgca ggaccgcggg gatgccggac gtggcggcga cggcgaggcc tacggcggtg 3601 agggtgctga gcgcgatgtt cacgtacagg ccgcggaccg gaccgccgac cgccctcgcg 3661 gaccgggcgt cgacgaccgc gcaggccacg tcgaacaggg gatagaccac cagcagggcc 3721 gcgctcagcg gaccgagggt gtcggcggtc gcgaagagca ggcccgccca tacggcggcg 3781 aacgcgaacc gcaggaagta cagcttccgc agggacgcgg cggcgccggt gacgggggtg 3841 gcgggggcgg tgcctcgggt ggtgacggag gtgtccacgg gggtgccttt cggtggcggc 3901 gcatcgatgg ggatgcgcgg gggagaacgt tcggtctctt gtggacatca aagggtgctc 3961 cgggatggct gtcaagaccg aacgttctcc ctcacctgtt tggtagcctc gggagcatga 4021 gtaggggcac aggaagcggc agcacgtccg aggcgcgagc caggctgctc ggtacggcga 4081 cgaggatctt ctacgcggag ggaatccact ccgtcggcgt cgaccgcatc atcgcggagg 4141 cgcaggtgac ccgggcgacg ctgtaccggc acttctcggg caaggaggag ctcgtcctcg 4201 cctacctcga ccaggccgac cgggggatcc gggcgcaggt cgcgtccgcg caggcggagg 4261 acgcgcccgc ggcggagcgg atccgggccg tcagccgctc catcaccgaa ggcatcaggt 4321 cacccgggtt ccgcggatgc gccttcctca acgcggtggc cgagtacccg gaccccgccc 4381 atccgatcca ccaggccgtg ctggcccacc ggcagtggtt cctggacacc gtcacggagt 4441 tgctggcgga ggcgggtcac acgcccgccg gtgcggccgg ccggcatctc gtgatgctgc 4501 gggacggcgc catggcggag ggctgcctct tcgacccgga gctcgtctcc gagacgttcc 4561 tgcaggccgt cgaggaactg ctgcgggcgc gccccgcctc gacggggcga gtgacagctt 4621 gacggggccg gtcgtggggg ccgggggcgg cgcctctcga ccggccggtg tgctcacggt 4681 cgagtcgtct cgactcgtcg gtgtgctcac ggccgagtcg tacggctcat gtcggagacc 4741 cgtcgtccgc ctccgggacc cgggctgccc gcgtggctac ctggaggggt gtggggcgcg 4801 agcgtggcgc cgccccacgg ttgtccgggc cgccgggccg aggtgacact gtgtgtgggg 4861 tgcgagcgtg gcgccgcccc acggttgtcc gggccgccgg gccgaggtga cactgtgtgt 4921 ggggtgcgag cgtggcgccg ccccacggcc gctatgcccc gcggcctgac cggcatcggg 4981 gcatacgggc tcgggtcggc tcaggccgag agcgtggagg tggagccgag cccggcgaca 5041 cccaggccgt cgacgccgag ggcgccggag aatcccgcgg agatcgagga gagcggcgtc 5101 tcgagggcga cctcgccggc caggccggcg aaggtgccac cggacacgtt gtccaggtcc 5161 gcgtcggaga tttcctgggc ctgtatctgg ggggcggagc tcatgaggtg aattcctttc 5221 acgttctgct tccgaatggc gggaggccga ccccgtcagg aaagaaggcc ccattgccgc 5281 tgtcacgact gcggcttccg cggtgcgaag gatgaaagca cgtggacgcg tcacgaggcc 5341 acccacccgg cagcgctccg gcctgctctt cctcaccgaa aaggcagggc ggtcacatcg 5401 gttcgtggac cggtgcgaat tcttcacccg acaggacggc tgaatttccg tcggccgtat 5461 tcctgatccg ccgcgaacgg gacattcccc gtaccggagg cgaggcgttc tccccgcgcc 5521 gggagaacgg ccgtcacctg cgtgcgccgt gtggagatcc accgcagacg ggccggcgac 5581 ggccttctcg gacccaggtc agggcccgac cggcgtgccc ggagcgatgt cgtacgccct 5641 ctgtgcgcgg atacgggcat ttcggtcacc ctcggcagcg gtgggagcgt ggggtccgag 5701 gtcaggaccg gtggcgtacg aaccggatat aggcgtagct ctccccctca cgcggcgccc 5761 gggtccagga ccgggtggtg aaggtgctca ggcgcccccg ggtcagcgcg aagcggggca 5821 gggacaggcc gagtttggtg tgcagggccg ccagcgcgcg cagctcgtta tcccgttcgg 5881 tggcgtccgg gaggaacagg cctgcggggt ccagggcgtc gggtatcgct ccggagcgct 5941 ggatctccgt accccgcacc gtgaacgcgt agagctcctc accctgttcg gccaccgaca 6001 ggtggaacgt gggcgggcgg tcgcccggcg acgggcggcc cggttcgtgc cacaccacca 6061 ccgcacggcc ggacgcggag gcggccgcgg cgggggacac gaaccgctcg cccaggtggt 6121 gagggtcgcg gtcgaaggcg aaggcccacc cgccggcggt gcggccgacg gcgaccgggg 6181 cccggtcctc ccagggctcc gcgtgctcct gcgtctcggc cacggatgcc cgccgccagc 6241 gcccccagcg ctgatccatg gcggcgctca gcgtggagcc ctcctcgccg atcagacccg 6301 gcagccgggc gggatcgatc ccctcggccc agacacagcg gtatccgtcg tcggcctgcc 6361 ggtacggatc ggattccgtc agccacgcga gacccggcgg gtcgagatcg ggcacgggat 6421 ccggagcagg gccctgctcc ccggcccgag gggtcgcgag gatctcccgc ccgcgctccg 6481 gagtgaccag cggccccagg aacggatcgg cgagcaggcc gaccggggcg atcagctggg 6541 gtccgggggc ttcccacagc gggagcgccg cccgcagcac ccgccaggcc gcgtcggtcg 6601 caccccagcg ggccgcttcc cgggcctcgg cgacggcccg gccccacggg ccgggaggcg 6661 cataccggtg ggtgccgtcc cgcacggcgc ccagtacggc ctcggcggtc tcccggggga 6721 ccccgcgggc ggccatcatg ccgagccagt gggcgtcccc gtccggcccc cgttcgccgc 6781 gcgccggttc catggcctgc aggggaagta tctcgggcag acagctcggg tcggccacca 6841 ggaagccgta gtcccgggcg gtctgcggag ccgtcaggtg ccggagctgg ttcagcaggg 6901 tggcgctgtg cggccgaccg aaggagagcg cgtcctccag caggggcagg gcctcctcgt 6961 gccgcccgcg cagggccagc agacgggcct cctcgacatg ggcgtcctgg gcgcgggtgg 7021 tcgcgttcac gaagccgggc atccccgccc ggtccccgtg gaagccgcgg tacatggcct 7081 ccatgtacgc gcggaacgac gggtagcggt ccgggtactc gccgctccag cccttgtaga 7141 cgtagagcgc ccactcgccg tcctggtccc ggtcgcccgg gtcgaggagg gccgacgaca 7201 tgtcggactc cgtctccagc cgcagcgccc gctgccacat accggcgagc aggacctcct 7261 cctcgcgcgg gtcgtcgccc agaccctcct cgtacagcgg ggtcatgccg tgcggatcat 7321 ggaaccagtc gatgtccgcg gcgcccccga gctcgtagat cgcggctgtc tgctccacgc 7381 gccacccgtc gctcgcggcc aggaactccc ggtaggacgg cggcagccgc tgccccagcc 7441 gtctctcggc cgcggcgatc gcggcctcgt ccgctccggg tctggccggg ccggcgaggg 7501 gcggctcgtc gtcctgctcg tcgtcctcgc cgggaaccca ctcctcctgc caacggccca 7561 ggaagtcgcg ccagtcgaaa gtctcatcgc tcatggccag gatgctgcca tctgccaccg 7621 acaacggcgc cggcaccagg gatccggtcg gcgccgaccg gattcgaacc ggcgtcctcg 7681 cggttgtgga gaccggcgcg acgacctctg cgctacggct ccgcccggcc cggcatgcta 7741 gcaaccgtcc gttcgcctgt gccgacggtc agttcgctca cccccgcggg tttctccgcg 7801 tccgccgagc gcaccgtgag cctgacccgg cgggccggat cgcccgggtg gtacgggcgc 7861 cacgcgcggc cgtccaggga ggtctccagg gtgtgggagg ccggtgcggt gtccgtccac 7921 cggggcgtca gcgaagcgat ccgggtcacc tgacgcaggg tcaccgtcag cgtgcccgtc 7981 gcgccgtccg gtgaccagga ggtcgacggg ctgccgtcca ccgccgcctc ggcgtacagg 8041 ccgggcgcct cggacgtggc cgtcgccggg cggcagcggg ccgcgtcggt cgtcggggcg 8101 aggtcgggcc gccgggtggg gagcgtgagg gtgccggaga ggcgtcgcgg ccccgccggg 8161 gtgtggacgg tgaagggcgc gcccgccgtc agccggaccg tggtcgtccg ggggccgacg 8221 gcgacgtcgt acgtacggcc ccggtagtgc agccccgtca gctccacgcc ctcgcggagc 8281 tgcggcggca ggagcgggtc caggcgtgcg ccgtcctcgc gcagcctgag cccggtcaga 8341 ccgtgcgtga acacctggag gaagccgccc ttgccggtga ggaagtcctc ggccgggaag 8401 cccgagagcg ggtcctcggc cccggacttc tcgccccgcg cctcggagaa gaggctgtac 8461 gggccccgga cgaacgggcg tacggcgcgc tggagatacg tgtacgtggc gcagcccggc 8521 tccccggtcg cggcggcgtc gatcgcgtgc accgagtcgg tcatcgcggg gccgtccggg 8581 tcggtgcggg cagcgtagta atcgagcgtc gcggcggcgg cgccctcctc catcggccac 8641 tccagcgggt agaccagcag caccgtgtcg gcctgcttga tcgtcgagcc gttgtagccg 8701 gcgtactgga ggaacagctt ccgatcggcg tcgtacggga tgcgcagccc gtcggcgacc 8761 cgggtccagc cggcgggcgc cgggtggccg aggagggctg cggcgcgggt ggcgtggcgc 8821 agggcagtgg cggcgaccgc gttggtgaag acgccgtcgg tgacgccgtt gctgtactcg 8881 tcggggccgg cgacgttctt caccgagtag ctgccgtcgt cgttggccgt ggcgcgcgac 8941 tgccagaagt cggcgatgcc cttcagcagc ggccagcccc gctcggccag ccaggcgcgg 9001 tcgccggtgg ccaggtagta ctgccagacg gcgagtgcga tgtcgccctg gaggtggttc 9061 tgggtgaggc agtgcggcgg gtcccagctc tggcactcgg agtccagacg gcccttgctc 9121 gcgctcgtcc aggggtagaa cagccccttg tagcccagct tcccggcgtt cgcgcgggcg 9181 gcggcgcggg tgcggtagcg gtactcgacg accgagcggg ccaggtcggg gcgggtggcg 9241 agcagccccg gatacatcca ggtctcggcg tcccagaaca ccatgcccgc gtagttgtcg 9301 ctggtcagcc cggccggggc gatgctgtcc gaggagccgg gccgggtgtt ggcgagcagt 9361 ccgtactgcg cggcccgcag ccaggcctgg aggtccgggc tgcccggggt ccggacgtcg 9421 gacgcccacg cctcgcgcca ggccgccgcg ttggcggcga ggaccccgga ccagccgcgc 9481 cgggccgcgc ggtccgccgc cgcaccggcc gagacgagcg gggtgcggga ggtgagggcg 9541 gtgtcgacgc ccacgtactt ctcgaaggtg tacgagcgcc ccgccctgac ccgggtggta 9601 cgcgcgccgt cggtgccccg ctggacgatc gccccctccg tgccggtgcc gagcgtccgg 9661 aacgtgccgt ccccggcgac cgtgacccgg cgcgccccgc gcgcgtccag ccggcccgtg 9721 accgtcgcgg tgccgctcca gtgcggggtc atccgcaggc gtacggcgcc ggtgtggacg 9781 tcggagcggt cggccagcac ctcgtaggtc aggtcggtcg cacgcccgtc ggccgtggtc 9841 caccgcaggg acgtgatcac gacgccgcag cgcaggtgca gggtctggcg gtagcgggac 9901 acacggccgg cgggggtggc ggagccgtac gtctcggagc cgacgcgcac gtcgagcgtg 9961 gtccaggacg gcagcgcggc gatcacctcg cggccctcgg cgagggcttc gtcccgggcg 10021 tggagccccg aaacgaaggc accgtcgtac cgtggggtgt agaggggcca gcccgtcttc 10081 tcgcccgtgg cggcgtatcc ggcgccggtg gcgggcaccc ggtggcccag gtagccgttg 10141 ccgacatagg ggtcgtatcc ggcggcctcg ccgaaggtgg tggacgtcgg tgcccaggag 10201 gcgt //