LOCUS       NZ_RDBM01000019         7973 bp    DNA     linear   CON 01-JAN-2025
DEFINITION  Streptomyces sp. gb1(2016) Scaffold_19, whole genome shotgun
            sequence.
ACCESSION   NZ_RDBM01000019
VERSION     NZ_RDBM01000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. gb1(2016)
  ORGANISM  Streptomyces sp. gb1(2016)
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 52540)
  AUTHORS   Hariharan,J., Choudoir,M.J., Diebold,P., Panke-Buisse,K.,
            Campbell,A.N. and Buckley,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2018) Soil and Crop Sciences, Cornell University,
            306 Tower Road, Ithaca, NY 14850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: A5+MeDuSa v. JUNE-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008042065.1-RS_2025_01_01
            Annotation Date                   :: 01/01/2025 02:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,414
            CDSs (total)                      :: 7,333
            Genes (coding)                    :: 6,887
            CDSs (with protein)               :: 6,887
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 447
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 447
            Pseudo Genes (frameshifted)       :: 94 of 447
            Pseudo Genes (incomplete)         :: 395 of 447
            Pseudo Genes (internal stop)      :: 7 of 447
            Pseudo Genes (multiple problems)  :: 48 of 447
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:17:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 44567
            Orig. end                         :: 52540
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RDBM01000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..7973
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..7973
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7973
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5912](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5912)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(700..715)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.19"
                     /tool="antismash"
     misc_feature    808..823
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.57"
                     /tool="antismash"
     misc_feature    1101..1111
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    complement(1162..1177)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.11"
                     /tool="antismash"
     gene            1626..1934
                     /locus_tag="EAO74_RS04930"
                     /old_locus_tag="EAO74_04940"
     CDS             1626..1934
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092371.1"
                     /locus_tag="EAO74_RS04930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_04940"
                     /product="SCO5918 family protein"
                     /protein_id="WP_124277799.1"
                     /transl_table=11
                     /translation="MRCVIARFPFDFFKNEVEDLMKGVKPEPVTGDAVVVNRRVYPVKQ
                     VGEIITRQDRRDFSAGEVTRALSRLGFTCRPAEVPVPVAPLTPLENASAMLGTPGEA"
     gene            complement(2008..3084)
                     /locus_tag="EAO74_RS04935"
                     /old_locus_tag="EAO74_04945"
     CDS             complement(2008..3084)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155343.1"
                     /locus_tag="EAO74_RS04935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_04945"
                     /product="acyltransferase family protein"
                     /protein_id="WP_124277798.1"
                     /transl_table=11
                     /translation="MPSLTSRTPGPAGADAGEPAAAQSRPGRDPFFDNAKYLAIVLVAM
                     GHSWEPLPGSGRLVETLYTFVYTFHMPVFIIIAGYFSRGLELSSDRVWRLVTSLAVPYA
                     VFEVAYTLFERLTGSPEFPLSPLTPYWLLWFLPALFLWRLTTPFWLRVRWPVALALVIA
                     AMGAVTSNVGEDLQFQRILQFLPYYVLGLCLRPEHFDLVRRRAVRLASVPVCLVALAVA
                     YWAVPRSTTSWFFRRYGAEQLGSPAWTGAVVTPLLFGCALVLSACFLAWVPNRRLWMTA
                     LGAGTLYGYLLHGFFIKGAVKLGLYEVDVVSTPLGRVLVSAVAAAVVTALCSPPVRRVF
                     RHVVEPRLDWARKPVPKS"
     gene            complement(3123..4031)
                     /locus_tag="EAO74_RS04940"
                     /old_locus_tag="EAO74_04950"
     CDS             complement(3123..4031)
                     /NRPS_PKS="Domain: PKS_ER (20-300). E-value: 8.9e-38.
                     Score: 122.1. Matches aSDomain:
                     nrpspksdomains_EAO74_RS04940_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155344.1"
                     /locus_tag="EAO74_RS04940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_04950"
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="WP_147982786.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 6.4e-19, bitscore:
                     60.0, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLAIQFDRFGGPDVLRPVELAAPVPGPGEVLLTVEAAGVNFADTH
                     QVDGSYLGADALPYVPGSEVVGRTPDGRRVLARVSHGYAEQVTAKESAVLEIPEDLGAA
                     QALALMMQGLTAWHLLRSAARLREGETVVVHSAAGGVGSLAVQLAREFGAGRVLAQAST
                     PEKERLALDVGADAVAAYPLAEKADVVLDAVGGDLFDQALGSLASFGRLVTYGNAARSG
                     FTPVDPARLGRLNASVVGFWLRPALAAPGGVSGPLKELFALAGEGRLRPVTGGSYPLAD
                     ARRAHEDLLARRTTGKLILTP"
     aSDomain        complement(3132..3971)
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS04940_PKS_ER.1"
                     /evalue="8.90E-38"
                     /label="EAO74_RS04940_PKS_ER.1"
                     /locus_tag="EAO74_RS04940"
                     /protein_end="300"
                     /protein_start="20"
                     /score="122.1"
                     /tool="antismash"
                     /translation="AAPVPGPGEVLLTVEAAGVNFADTHQVDGSYLGADALPYVPGSEV
                     VGRTPDGRRVLARVSHGYAEQVTAKESAVLEIPEDLGAAQALALMMQGLTAWHLLRSAA
                     RLREGETVVVHSAAGGVGSLAVQLAREFGAGRVLAQASTPEKERLALDVGADAVAAYPL
                     AEKADVVLDAVGGDLFDQALGSLASFGRLVTYGNAARSGFTPVDPARLGRLNASVVGFW
                     LRPALAAPGGVSGPLKELFALAGEGRLRPVTGGSYPLADARRAHEDLLARRTTGKLIL"
     CDS_motif       complement(3585..3647)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EAO74_RS04940_0001"
                     /evalue="1.80E-07"
                     /label="PKSI-ER_m5"
                     /locus_tag="EAO74_RS04940"
                     /protein_end="149"
                     /protein_start="128"
                     /score="23.6"
                     /tool="antismash"
                     /translation="GETVVVHSAAGGVGSLAVQLA"
     gene            4232..4996
                     /locus_tag="EAO74_RS04945"
                     /old_locus_tag="EAO74_04955"
     CDS             4232..4996
                     /EC_number="1.1.1.47"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (8-171). E-value: 4e-16. Score:
                     51.4. Matches aSDomain:
                     nrpspksdomains_EAO74_RS04945_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155345.1"
                     /locus_tag="EAO74_RS04945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_04955"
                     /product="glucose 1-dehydrogenase"
                     /protein_id="WP_124277796.1"
                     /sec_met_domain="adh_short (E-value: 3.8e-37, bitscore:
                     119.7, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 5.1e-35, bitscore:
                     113.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDLLHDRVVLITGASSGIGAAAARVFSEEGATVVLAARREDRLA
                     ALTAELKDKGAQASYVVCDVTVAEDAARAVDFTVDTYGRLDSAFNNAGLGGDRTPLHLM
                     GDDVYDAVMDTNVRGVWNCLRHEIAAMLPNSGGSSIVNNSSVGGLVAIPAAAPYIAAKH
                     AVVGLTRAAADEYAQQGIRVNAVAPGTTRSEITADWFGRNPGLEEMVNSRTPQGRTAEP
                     EEIANAVAWLLSDRCPFLTGTVLPVDGGFINQ"
     CDS_motif       4256..4351
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_EAO74_RS04945_0001"
                     /evalue="5.10E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="EAO74_RS04945"
                     /protein_end="40"
                     /protein_start="8"
                     /score="12.7"
                     /tool="antismash"
                     /translation="VVLITGASSGIGAAAARVFSEEGATVVLAARR"
     aSDomain        4256..4744
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS04945_PKS_KR.1"
                     /evalue="4.00E-16"
                     /label="EAO74_RS04945_PKS_KR.1"
                     /locus_tag="EAO74_RS04945"
                     /protein_end="171"
                     /protein_start="8"
                     /score="51.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVLITGASSGIGAAAARVFSEEGATVVLAARREDRLAALTAELKD
                     KGAQASYVVCDVTVAEDAARAVDFTVDTYGRLDSAFNNAGLGGDRTPLHLMGDDVYDAV
                     MDTNVRGVWNCLRHEIAAMLPNSGGSSIVNNSSVGGLVAIPAAAPYIAAKHAVVGLTRA
                     "
     gene            complement(5001..5912)
                     /locus_tag="EAO74_RS04950"
                     /old_locus_tag="EAO74_04960"
     CDS             complement(5001..5912)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577826.1"
                     /locus_tag="EAO74_RS04950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_04960"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_147982787.1"
                     /sec_met_domain="AfsA (E-value: 2.3e-18, bitscore: 57.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDIPVAPRPPHSAAEGRNGVPKEYVHLGRTEMVFLTTWHSREDG
                     EFSLSARWPAPAEGLPYDPRVLTQTIRQSGLIIAHAAYGVPLTDQTLLHYFDFTVAPGF
                     RVPADGPCGLDVEVSVAEPKKRGRAVSSLGMDVRVLQGGSLVARADSEFGWISPRAYRR
                     LRGEHLAVDWNAWPLPAPVDPRTVGRHTPHDVILSAGDRPRHWQLRNDVDNVVLYDHPV
                     DHVPGLVLMEAAHQAANASLHPAPFEATTVTSEFMRYAEFDRPCWIEAEILPAGPGETS
                     VLVTAAQDGETVYRGRLSGPCG"
     misc_feature    5975..5984
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.37"
                     /tool="antismash"
     misc_feature    5976..5989
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.78"
                     /tool="antismash"
     gene            6019..6663
                     /locus_tag="EAO74_RS04955"
                     /old_locus_tag="EAO74_04965"
     CDS             6019..6663
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155347.1"
                     /locus_tag="EAO74_RS04955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_04965"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_099173389.1"
                     /transl_table=11
                     /translation="MARQQRAIRTRRIFLEAAAEVFDEHGYDAATIAAILDRAGLTRGA
                     LYFHFTSKEELARGVLQEAVTADGVTPQTFKLQEWVDIALLLAYRLPREPLLSASIRLS
                     VDPRARGLFGTRWPDWIALGTEILTEARERGELLPHVDPEATSRLFVGAWTGVQLVTES
                     MAEPPDLVAEISALFELILPNNAVPGVLARLDTSPHRAARIIEQQQAVAVS"
     gene            6768..7502
                     /locus_tag="EAO74_RS04960"
                     /old_locus_tag="EAO74_04970"
     CDS             6768..7502
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047019.1"
                     /locus_tag="EAO74_RS04960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_04970"
                     /product="peptidoglycan recognition protein"
                     /protein_id="WP_124277794.1"
                     /transl_table=11
                     /translation="MWLRRTAVCAMVVPLAFLVLRDAPVRLAAPERTTAKPAAPPAAPR
                     PAIVSRAEWHADESLVKEDAVYTGTVRAVFVHHTGHTNNYDCADAPEMLRAMEEAHVRG
                     EGWDDIGYNFVVDRCGTIYEGRAGGVGRTVRGAHATGFNAHSVGIAALGTFDPGVTVPK
                     ALIEGIAKVGAWKLDRSIDPRGIVRMVSTNDDSLYRRGEVVYLHAVAGHRDTFSTYCPG
                     EALYAQLPAIRAEMARLRDRKK"
     gene            7712..7912
                     /locus_tag="EAO74_RS39155"
                     /old_locus_tag="EAO74_04975"
     CDS             7712..7912
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="EAO74_RS39155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="EAO74_04975"
                     /product="hypothetical protein"
                     /protein_id="WP_315985651.1"
                     /transl_table=11
                     /translation="MPASPLAKRPVESVLALLLVLLSVLGLAGRSVADAPAQAPARTRS
                     ATAICSDLEARSARGAYCPPP"
     gene            complement(<7882..7971)
                     /locus_tag="EAO74_RS04970"
                     /old_locus_tag="EAO74_04980"
                     /pseudo=""
     CDS             complement(<7882..7971)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018447.5"
                     /locus_tag="EAO74_RS04970"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_04980"
                     /product="transglycosylase family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MNTGNGYHGGLQFAPSTWRAYGGGQYAPRA"
ORIGIN
        1 gcgatcgcac atctcgcacg aacgaccgat tttcccggac gcgttccggc ggatccaccg
       61 gcagccgtac tgccaacgga ttccgcggca cggcatccgg ccgtcagggg gccccgggtt
      121 cgcagggccg ctccggcggg ccgaagcgct ccggtggtgg ctacggccgc cgctcctcct
      181 ccgtctcggg tgagttcgcc ctgccggtga ccatcacccc cgccctcccg gccgccgagt
      241 ccttcgcgga cctggacatg cccgcgcccc tcctggcggc gctggtctcc gagggcatgt
      301 cggtgccgtt ccccatccag gccgccaccc tgccgaactc gctcgccgga cgggacgtcc
      361 tgggccgcgg ccgcaccgga tcgggcaaga cgctcgcctt cggcctcgcg ctcctggccc
      421 gcaccgcggg caaccgcgcc gacgcccgcc gtccgctggc cctggtcctc gtccccaccc
      481 gggaactggc ccagcaggtc accgacgcgc tcaccccgta cgcccggtcc ctcaagctcc
      541 ggctggccac ggtcgtcggc gggatgtcga tcggccgcca ggccagcgcc ctgcgcggcg
      601 gcgccgaagt cgtcgtcgcc accccgggcc ggctgaagga cctcatcgag cgcggcgact
      661 gccggctgga ccgcgtcgcc atcaccgtcc tcgacgaggc cgaccagatg gccgacatgg
      721 gcttcatgcc gcaggtcacc gagctgctcg accaggtcaa cgccgacggc cagcggatgc
      781 ttttctcggc caccctggac cgcaacgtgg acctgctggt ccgccgctac ctgcacgacc
      841 cggtcgtcca ctccgtggac cccgcggccg gtgcggtgac gacgatggag caccacgtgc
      901 tctacgtcca gggcgccgac aagtacgcga cgaccaccga gatcgccgcc cgtgagggcc
      961 gggtgatcat gttcctcgac accaagcacg cggtggacaa gctcaccgac cacctgctgc
     1021 acagcggtgt ccgggcggcc gcgctgcacg gcggcaagtc gcagccgcag cgcacccgca
     1081 ccctggaccg cttcaagacc ggccatgtca cggtgctggt cgccacgaac gtcgccgccc
     1141 gcggcatcca cgtcgacaac ctcgacctgg tcgtcaacgt ggacccgccc agcgaccaca
     1201 aggactacct gcaccgcggc ggacgtaccg cgcgcgccgg ggagtccggc agcgtcgtca
     1261 cgctggtgct gcccaaccag cgccgcgaga tgacccggct gatggccgac gcgggcatca
     1321 cgccgcagat cgcccaggtc cgctccggcg aggccgagct caaccgcatc accggtgccc
     1381 aggccccctc gggtgtgccc gtcaccatca cctcgccggt gtcggagcgc aagagcggca
     1441 gttccggccc ccggggccgg cgcggcgcgc gtcccggcca ggggcgccgt tccaacgcca
     1501 acacgcccac gccgcaggcg cgagccgctt cggcgcagcg ccgggccaac aaggccgcgt
     1561 agtcacccgg ccgggagccc ggccgtcctg tccggttttc tccacgacac ccttgttgag
     1621 gcaccatgcg ctgtgtcatc gcccgcttcc ccttcgactt cttcaagaac gaggtcgagg
     1681 acctgatgaa gggcgtcaag cccgaacccg tcacgggtga cgcggtggtc gtcaaccgcc
     1741 gtgtgtaccc cgtcaagcag gtgggcgaga tcatcacccg ccaggaccgc cgggacttct
     1801 ccgccggtga ggtgaccagg gcgctgtcgc gtctcggctt cacctgccgc ccggcggagg
     1861 tgcccgtccc cgtggcgccg ctcacgccgc tggagaacgc gtccgccatg ctcggaacgc
     1921 ccggcgaggc ctgagccgcc gctccctccc gcgcaccgca cccccgaggc cttcccggcc
     1981 cgggggtgcg gtgcgtcctg gggggcctca ggacttcggc acgggcttcc tggcccagtc
     2041 gagccggggc tccacgacgt gacggaacac gcgccgcacg ggagggctgc agagggcggt
     2101 gaccacggcc gcggcgacgg cggagacgag gacccggccc aggggcgtgg agacgacgtc
     2161 cacctcgtac aggccgagct tgaccgcgcc cttgatgaag aagccgtgca gcagatagcc
     2221 gtagagcgtg ccggcgccga gcgcggtcat ccacagcctg cggttcggca cccaggcgag
     2281 gaagcaggcg ctgagcacga gcgcgcagcc gaacagcagc ggcgtcacca cggcgcccgt
     2341 ccaggcgggc gagccgagct gctccgcgcc gtaccggcgg aagaaccacg aggtcgtcga
     2401 gcgcggcacg gcccagtagg cgaccgccag ggccaccagg cagaccggta cggacgcgag
     2461 gcgcaccgcc cggcggcgta cgaggtcgaa gtgctcgggg cgcaggcaca gccccaggac
     2521 gtagtacggg aggaactgca ggatccgctg gaactgcagg tcctcgccga cgttgctggt
     2581 caccgcgccc atggcggcga tgaccagggc cagtgcgacg ggccaccgga cccgtagcca
     2641 gaagggtgtg gtgaggcgcc agaggaacag cgcgggcagg aaccagagca gccagtacgg
     2701 cgtgagcggg ctcagaggga actccgggga cccggtcagg cgctcgaaga gcgtgtaggc
     2761 gacctcgaag acggcgtagg gcacggcgag gctcgtgacg agcctccaca cccggtcgga
     2821 gctcagctcc aggccgcgcg agaagtatcc ggcgatgatg atgaagaccg gcatatggaa
     2881 ggtgtagacg aaggtgtaga gcgtctccac cagtcgcccg ctgccgggga ggggctccca
     2941 ggagtgcccc atcgcgacga ggacgatggc caggtacttc gcgttgtcga agaacgggtc
     3001 gcggccaggg cgggactgcg ctgcggccgg ttctccggcg tcggcgcctg ccggtcccgg
     3061 ggtgcgtgag gtcagggagg gcatcgggcc accctagggc ctcccgcccg gaccgggcgg
     3121 gttcagggcg tcaggatcag ctttcccgtg gtccggcgcg cgagcaggtc ctcatgggcg
     3181 cgccgggcgt cggcgagcgg gtagctgccg ccggtcacgg gcctgaggcg gccctcgccg
     3241 gcgagggcga agagctcctt gagggggccg ctgacgccgc ccggggccgc gagggcggga
     3301 cgcagccaga atccgacgac ggaggcgttg agacggccga gacgggccgg gtcgaccggg
     3361 gtgaagccgc tgcgggcggc gttgccgtag gtcaccagcc ggccgaagga cgccagggag
     3421 cccagggcct ggtcgaagag gtccccgccc acggcgtcca ggacgacgtc cgccttctcg
     3481 gcgagggggt aggcggccac cgcgtcggcg ccgacgtcca gggcgaggcg ctccttctcc
     3541 ggggtggagg cctgggcgag cacccggccc gcgccgaact cccgggccag ctggacggcc
     3601 aggctgccga cgccgcccgc cgcggagtgc acgacgacgg tctccccctc gcgcagccgg
     3661 gccgccgacc gcagcagatg ccaggcggtc agaccctgca tcatgagggc gagggcctgt
     3721 gccgcgccga ggtcctcggg gatctccagg acggcggact ccttggcggt cacctgctcc
     3781 gcgtacccgt gggagacccg ggccagcacc cgccggccgt ccggggtgcg gcccacgacc
     3841 tcgctgcccg ggacgtacgg cagggcgtcg gcgccgaggt aggagccgtc cacctgatgg
     3901 gtgtccgcga agttgacccc tgcggcctcg acggtgagga gcacctctcc ggggccgggc
     3961 acgggggcgg ccagttccac ggggcggagg acgtcggggc caccgaagcg gtcgaactgg
     4021 atcgcgagca tgccgcaggg tatcgcggag ccgtccggcc gggccggggc ccggtggcag
     4081 gccgtcggaa tccggtgagc ggggaggcga aaacaggaca atcggtcggt tatggtcgtg
     4141 aggccggtcc ggagcccggg gggtgggccg gccggtgctc cgggcgtatc ggccgtggtc
     4201 gtggagcggt gcgcctgcca gactgcgggc catgagcgat cttcttcacg acagagtcgt
     4261 cctgatcacc ggggcgagca gcggtatcgg cgcggcggcg gcacgggtgt tctccgagga
     4321 gggcgcgacg gtcgtcctgg ccgcgcgccg ggaggaccga ctggccgcgc tgacagccga
     4381 attgaaggac aagggggccc aagcctccta tgtcgtctgc gatgtcacgg tggccgagga
     4441 cgcggcccgt gccgtcgact tcacggtcga cacctacggg cgcctggact cggccttcaa
     4501 caacgcgggc ctcggcggcg accgtactcc cctgcacctc atgggagatg acgtctacga
     4561 cgcggtcatg gacaccaacg tgcggggcgt ctggaactgc ctccgccacg agatcgcggc
     4621 catgctgccc aattccgggg gcagttccat cgtgaacaac agcagcgtgg gcgggctggt
     4681 ggccataccg gcggccgcgc cctacatcgc ggccaagcac gccgtcgtcg gtctcaccag
     4741 ggccgccgcg gacgagtacg cccaacaggg catcagggtc aacgcggtgg cgcccggcac
     4801 cacccgcagc gagatcacgg ccgactggtt cggccgtaac ccggggctgg aggagatggt
     4861 caacagccgt acgccgcagg ggcgtacggc tgagcccgag gagatagcca acgcggtggc
     4921 ctggctgctc agcgaccgct gccccttcct cacgggcacc gtgctcccgg tggacggcgg
     4981 cttcatcaac cagtgaggcg tcagccgcag ggcccgctca gccggccccg gtagaccgtc
     5041 tccccgtcct gcgccgcggt caccaggacg gaggtctccc cggggccggc gggcaggatc
     5101 tccgcctcga tccagcaggg gcggtcgaac tcggcgtacc gcatgaactc actggtgacg
     5161 gtggtcgctt cgaagggcgc gggatgcaga gaggcgttcg cggcctggtg ggcggcctcc
     5221 atcaggacga gccccgggac gtggtcgacg gggtggtcgt agagcacgac gttgtccacg
     5281 tcgttgcgca actgccagtg gcggggccgg tctccggccg acaggatcac gtcgtgcggg
     5341 gtgtgccgcc cgacggtccg ggggtcgacc ggggcaggca gtggccaggc gttccagtcg
     5401 acggcgaggt gttcaccgcg gagccggcgg taggcgcgcg gagaaatcca gccgaactcg
     5461 gaatccgcac gggcgaccaa gctgccaccc tggagtacgc ggacgtccat tccgagtgaa
     5521 ctgaccgccc gtccgcgctt cttgggctcg gcgacggaga cctccacgtc gaggccgcag
     5581 ggtccatcgg cggggacgcg gaagcccggg gcgacggtga agtcgaagta gtggagcagg
     5641 gtttgatcgg tcaggggaac gccgtacgct gcgtgcgcga tgatcagtcc gctctgcctg
     5701 atggtctggg tgagcacccg ggggtcgtaa gggagccctt ccgcgggtgc gggccatcgc
     5761 gcggacagcg agaactcgcc atcttccctg ctgtgccagg tggtgaggaa gaccatctcg
     5821 gtacgcccga ggtggacgta ctccttgggg acgccgttcc ggccctccgc tgcggagtgc
     5881 ggtggtctcg gagcaacggg tatgtcgggc acggccctcc cggacaatgg aacaaaatgg
     5941 gcattacggc gctgatatga taccggttca ccggtacttt ttgaagaagc gtcaggccac
     6001 gttcaagggg agcgcgagtt ggcacgacag caacgggcga tccgcacgcg cagaatcttc
     6061 ctggaagccg ccgcagaggt cttcgacgag cacggttacg acgccgccac gatcgccgcg
     6121 atcctcgacc gggccggtct cacacggggt gcgctctact tccacttcac gtcgaaggag
     6181 gagctggccc gcggtgtcct ccaggaggcg gtgaccgcgg acggggtcac cccgcagacc
     6241 ttcaagctgc aggaatgggt ggacatagca ctcctgctcg cctaccggct gcccagggaa
     6301 cccctgctca gcgcctcgat ccggctgtcc gtcgacccac gggcgcgcgg gctgttcggc
     6361 acccgctggc ccgactggat cgccctcggc acggagatac tcaccgaggc cagagagcgg
     6421 ggcgagctcc ttccgcatgt ggacccggag gccacctcgc ggctcttcgt cggggcgtgg
     6481 accggggtcc agctggtgac cgagtccatg gcggagccgc cggacctcgt cgccgagatc
     6541 tccgccctct tcgagctcat cctcccgaac aacgcggtcc cgggggtgct cgcccggctc
     6601 gacacctccc cgcaccgcgc ggcgcgcatc atcgagcagc agcaggcggt cgccgtctcc
     6661 tgaccgtccg ggcggtgtca cgggcgagcc ccgggtcacc ccgccccatg tcacccgatc
     6721 gggtgtgggc gcctgcgaag tggctggtca gcccgcatgg tggccctatg tggcttcgcc
     6781 gaaccgccgt gtgcgccatg gtcgtgccgc tggcgttcct ggtactccgg gacgcgccgg
     6841 tacggcttgc cgcgcccgag cggaccaccg cgaagccggc ggcgccgccc gcggccccgc
     6901 gtcccgcgat cgtcagccgg gccgagtggc acgcggacga gagcctggtc aaggaggacg
     6961 cggtgtacac ggggacggtg cgggccgtct tcgtccatca cacgggtcac accaacaact
     7021 acgactgcgc cgacgccccc gagatgctcc gggcgatgga ggaggcgcac gtacgcggcg
     7081 agggctggga cgacatcggc tacaacttcg tggtcgaccg ctgcgggacc atatacgagg
     7141 gcagggcggg cggcgtcgga cgcaccgtgc gcggggcaca cgccaccggg ttcaacgcgc
     7201 acagcgtggg gatagcggcc ctcggcacct tcgatcccgg tgtcaccgtg ccgaaggcgc
     7261 tcatcgaggg gatcgcgaag gtcggcgcgt ggaagctgga ccgctccatc gacccccgcg
     7321 gcatcgtgcg gatggtgtcg acgaacgacg acagcctgta ccggcgcggt gaggtggtct
     7381 acctccacgc cgtggccggg caccgcgaca ccttctcgac gtactgcccc ggcgaggcgc
     7441 tctacgccca acttcccgcg atccgggcgg agatggcgcg gctgcgggac cggaagaagt
     7501 agcacgggag ccgtgcggcg cgacgtcccg ccccgcacct cgtggtgccg gcagcggctg
     7561 cgcgcggtca cgcggcgcgg cgaaccggcg ggccccccac ccgggtgatt gttcgggagt
     7621 cggccgcgtc gaagcccatt gaaaagatga tcggcggctt atctgttcgt gcccttgcac
     7681 accttctgga gcacagcgga ggaataccgt catgccggca tcacccctcg caaagcggcc
     7741 cgtcgaatcg gtgctcgccc ttctgctcgt cctcctgagc gtgctcggcc tcgccggccg
     7801 cagcgttgcg gacgcccccg cccaggcccc ggcccggacg cgctcggcga cggcgatctg
     7861 ctcggacttg gaggcgaggt cggcgcgggg cgcgtactgc ccgccgccgt aggcccgcca
     7921 ggtggagggg gcgaactgga ggccaccgtg gtagccgttg ccggtgttga tgc
//
