LOCUS       NZ_RDBM01000002         5612 bp    DNA     linear   CON 01-JAN-2025
DEFINITION  Streptomyces sp. gb1(2016) Scaffold_2, whole genome shotgun
            sequence.
ACCESSION   NZ_RDBM01000002
VERSION     NZ_RDBM01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. gb1(2016)
  ORGANISM  Streptomyces sp. gb1(2016)
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Hariharan,J., Choudoir,M.J., Diebold,P., Panke-Buisse,K.,
            Campbell,A.N. and Buckley,D.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2018) Soil and Crop Sciences, Cornell University,
            306 Tower Road, Ithaca, NY 14850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: A5+MeDuSa v. JUNE-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008042065.1-RS_2025_01_01
            Annotation Date                   :: 01/01/2025 02:23:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,414
            CDSs (total)                      :: 7,333
            Genes (coding)                    :: 6,887
            CDSs (with protein)               :: 6,887
            Genes (RNA)                       :: 81
            rRNAs                             :: 6, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 447
            CDSs (without protein)            :: 446
            Pseudo Genes (ambiguous residues) :: 0 of 447
            Pseudo Genes (frameshifted)       :: 94 of 447
            Pseudo Genes (incomplete)         :: 395 of 447
            Pseudo Genes (internal stop)      :: 7 of 447
            Pseudo Genes (multiple problems)  :: 48 of 447
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:17:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5612
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            RDBM01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..>687)
                     /locus_tag="EAO74_RS00050"
                     /old_locus_tag="EAO74_00050"
                     /pseudo=""
     CDS             complement(<1..687)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1090: acetyl-CoA carboxylase biotin carboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948354.1"
                     /locus_tag="EAO74_RS00050"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="EAO74_00050"
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /pseudo=""
                     /sec_met_domain="ATP-grasp (E-value: 4.4e-09, bitscore:
                     24.8, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAFALGGDTPAASYLDMAKVLQAAKDSGADAVHPGYGFLSENAEF
                     AQAVLDAGLTWIGPPPHAIRDLGDKVAARHIAQRAGAPLVAGTPDPVSGSAEVVEFAQE
                     NGLPIAIKAAFGGGGRGLKVARTLEEIPELYDSAVREAVAAFGRGECFVERYLDKPRHV
                     ETQCLADTHGNVVVVSTRDCSLQRRHQKLVEEAPAPFLSKEQNEQLYAASKAILKEAGY
                     VGAGTVE"
     source          1..5612
                     /collection_date="2009"
                     /db_xref="taxon:1828321"
                     /geo_loc_name="USA: Greensboro, North Carolina"
                     /isolation_source="soil"
                     /lat_lon="36.09 N 79.89 W"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. gb1(2016)"
                     /strain="gb1(2016)"
                     /submitter_seqid="Scaffold_2"
     region          1..5612
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..5612
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5612
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2437:3427](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(2438..3427)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    772..784
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 20.41"
                     /tool="antismash"
     gene            complement(<855..1121)
                     /locus_tag="EAO74_RS39300"
                     /pseudo=""
     CDS             complement(<855..1121)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012510.5"
                     /locus_tag="EAO74_RS39300"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRKVLIANRGEIAVRVARACRDAGIGSVAVYADPDRDALHVRAAD
                     EAFALGGDTPAASYLDMAKVLQAAKDSGADAVHPGYGFLSENAE"
     gene            complement(1359..2402)
                     /locus_tag="EAO74_RS00060"
                     /old_locus_tag="EAO74_00060"
     CDS             complement(1359..2402)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (90-197). E-value:
                     2.9e-08. Score: 25.1. Matches aSDomain:
                     nrpspksdomains_EAO74_RS00060_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546906.1"
                     /locus_tag="EAO74_RS00060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_00060"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_261988071.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.9e-13, bitscore:
                     38.2, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHTTMITGATGFIGRHVVEEARTREIPLRLMSRHRPPVMTTGRAA
                     PGDFGSGSAVRRNAGSGKNGPGNTVSGNPLTATVPAQGVVADLARPETLRGACDGVDVL
                     LHCASQIGGPADVNEAVNARGTAALVAEARRAGVRRIVYLSTASVYGRGTFRSARAEAL
                     DRHPGSVTSRTRAAAEDAVREAGGIVLRPHLVYGVGDIWVVPALARLLLALSGASAGWH
                     ARMSAVSAPELARLVLGVGSAPAGSLTASVYHAAHPRPVTAAELLRAVADCMGLPVPRR
                     ELTVAQVRERLVAAGLPQLALDMLATDHWFDSAPLWSDLGRVPGPGFAVDFAETREWYG
                     NTVRAAV"
     aSDomain        complement(1812..2132)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_EAO74_RS00060_NAD_binding_4.1"
                     /evalue="2.90E-08"
                     /label="EAO74_RS00060_NAD_binding_4.1"
                     /locus_tag="EAO74_RS00060"
                     /protein_end="197"
                     /protein_start="90"
                     /score="25.1"
                     /tool="antismash"
                     /translation="PETLRGACDGVDVLLHCASQIGGPADVNEAVNARGTAALVAEARR
                     AGVRRIVYLSTASVYGRGTFRSARAEALDRHPGSVTSRTRAAAEDAVREAGGIVLRPHL
                     VYG"
     gene            complement(2438..3427)
                     /locus_tag="EAO74_RS00065"
                     /old_locus_tag="EAO74_00065"
     CDS             complement(2438..3427)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627801.1"
                     /locus_tag="EAO74_RS00065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_00065"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_147982223.1"
                     /sec_met_domain="AfsA (E-value: 8.6e-25, bitscore: 75.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSLASAPYEPSRNIVLTPRPAPPIPELTTTVPRQYVHRASVSEV
                     LLTGWEAVADSAAGGAESFVVRAQWPRGHSLFSQVAGYQDPMLLIESVRQIGSLLSHAE
                     FGAPFGHQFLMWDMSFATTEEMLVAGPAPTEVELHTVCSDVVRRGRSLSSMRYDVTAVV
                     DGVALATGRAGFSCTSPAVHRRLRGDRPTSTGYVPGRPIDPARVGRNGAGHVVLADDGT
                     GAGAHRWELRVDTTHPIFFDHPVDHVPGMVLLEAARQAAHASTGLPDALVVALDSVFTR
                     YVELDTPCLIEAAPGRPDASGNVRVTVQGIQEGRAVYSAELVLRPRDC"
     misc_feature    complement(3377..3379)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    3543..3556
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.13"
                     /tool="antismash"
     gene            3974..4753
                     /locus_tag="EAO74_RS00070"
                     /old_locus_tag="EAO74_00070"
     CDS             3974..4753
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740558.1"
                     /locus_tag="EAO74_RS00070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_00070"
                     /product="response regulator transcription factor"
                     /protein_id="WP_099177744.1"
                     /transl_table=11
                     /translation="MNRQASDLLEPRTTQHRPLVSQPHRASALPHPPSAGERDVLRVLV
                     VENEERAADTLVQALLRQGYDAESVATGGKALQGHHQADLILLDLDLPDLDGLEVCRGI
                     RAVSDTPIITVTARGSELDRVLGLQAGSDDYMVKPYGFRELLARIEAVMRRSRPRQEIA
                     RTVDHGPLRIDATTREATLDGRQVDLTRKEFDLLHLLASQPGAVISRRQLMTQVWEDTW
                     SQRGRTIDTHVSSLRGKLGSSSWIITVRGVGFRLGHP"
     misc_feature    4202..4204
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4934..>5612
                     /locus_tag="EAO74_RS00075"
                     /old_locus_tag="EAO74_00075"
     CDS             4934..>5612
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546903.1"
                     /locus_tag="EAO74_RS00075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="EAO74_00075"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_147982224.1"
                     /transl_table=11
                     /translation="MGKQERGTRSRLSILEAAARVFDARGFDAASTNEILASTGLTRGA
                     LYHHFPSKEAIAVALLAAHDEALVVPDRQVKLQSVIDLTFEFAFRLQRDPVLRASVRLA
                     VEQTSFPRPVTTPYEQSGSAINELLKQAQQQGEILPGVDLVEATSIIIGAFTGMQVMSQ
                     MYTNRRDLPERVSTMWRFLLPGLANPGMISHLRITPAPESTPKPEPTPKPELERTGEPA
                     GSPA"
ORIGIN
        1 ctccaccgtg ccggcgccga cgtagccggc ttccttcagg atcgccttcg acgccgcgta
       61 gagctgctcg ttctgctcct tggacaggaa gggggcgggg gcctcctcga cgagcttctg
      121 gtgacggcgc tgcagcgagc agtcacgggt cgagaccacg acgacgttgc cgtgggtgtc
      181 ggccaggcac tgcgtctcca catggcgggg cttgtcgagg tagcgctcca cgaagcactc
      241 accgcgcccg aacgccgcga ccgcctctcg caccgccgag tcgtacagct cggggatctc
      301 ctccagcgtg cgggccacct tcagcccgcg cccgccacca ccgaaagcgg ccttgatcgc
      361 gatcggcaga ccgttctcct gcgcgaactc cacaacctcc gccgaacccg acaccggatc
      421 cggggtgccc gccaccaggg gggcgccggc acgctgggcg atgtgacggg cggcgacctt
      481 gtcacccaga tcccggatcg cgtgcggcgg cggaccgatc cacgtcagac ccgcatccag
      541 caccgcctgc gcgaactccg cgttctccga caggaagcca tacccggggt ggaccgcgtc
      601 cgcaccggaa tccttcgcgg cctgcagcac cttggccatg tccagatagc tcgcggccgg
      661 agtgtcaccg cccagagcga acgcctcacc acaacctccg ccgaacccga caccggatcc
      721 ggggtgcccg ccaccagggg ggcgccggca cgctgggcga tgtgacgggc ggcgaccttg
      781 tcacccagat cccggatcgc gtgcggcggc ggaccgatcc acgtcagacc cgcatccagc
      841 accgcctgcg cgaactccgc gttctccgac aggaagccat acccggggtg gaccgcgtcc
      901 gcaccggaat ccttcgcggc ctgcagcacc ttggccatgt ccagatagct cgcggccgga
      961 gtgtcaccgc ccagagcgaa cgcctcatca gccgcgcgca catgcagagc atcccggtcc
     1021 ggatcggcgt agacggctac gctcccgatc ccggcatccc ggcaagcccg agcaacacga
     1081 acagcgattt caccacggtt ggcgatgagc actttacgca cgatgaacgt ctcctgactc
     1141 gctcggagtc accagactgt tcgatcctcg ggcacctgtt caacacatcc ggttacagaa
     1201 ctgggtgcac attcccagga ctcgcccagc tgtgtcgaga gacgggcagg aaatggtcag
     1261 gcgcccgtca aaactctccg acgacctggg ccgttccgga aaaatgacgg gtgcggcagc
     1321 cggaccggcc gccgcacccc gggaaggacg gtgtcgattc agaccgccgc ccggaccgta
     1381 tttccgtacc actcccgtgt ttccgcgaaa tcaacggcga atccggggcc cggaacacgg
     1441 cccagatcgg accagagcgg ggcactgtcg aaccagtgat cggtggccag catgtccagc
     1501 gcgagctgcg gcaggcccgc ggcgacgagc cgctcccgca cctgcgccac cgtcagctcc
     1561 cggcgcggca cggggaggcc catgcagtcg gcgacggcac gcagcagctc cgccgcggtc
     1621 acgggccgcg gatgggcggc atggtagacg gacgcggtca ggctcccggc cggcgcggat
     1681 cccacgccca ggaccagccg ggccagttcg ggagcggaga cggccgacat acgggcgtgc
     1741 catcccgccg aggcgccgga cagcgccagc agcagccggg ccagcgcggg aaccacccag
     1801 atgtccccca ccccgtacac cagatggggc cggagcacga tccctcccgc ctcccgtacg
     1861 gcgtcctcgg cggcggcccg ggtcctggac gtcaccgaac ccgggtggcg gtcgagggcc
     1921 tcggcgcggg cgctccggaa ggtcccgcgg ccgtacacgg aagcggtgct gagatagacg
     1981 atgcgccgca cccccgcccg ccgggcctcc gcgacgaggg cggcggtccc ccgtgcgttg
     2041 accgcctcgt tgacgtcggc cgggcccccg atctgcgagg cgcagtgcaa caggacgtcc
     2101 acgccgtcac aggcgccgcg gagggtctcc ggccgggcga ggtcggcgac gacgccctgc
     2161 gcgggaacgg tcgcggtgag ggggtttccc gacacggtgt tccccgggcc gttcttcccc
     2221 gagccggcgt tcctcctcac cgcgctcccg gaaccgaagt cccccggggc ggcccgcccc
     2281 gtggtcatga ccggtgggcg gtggcgcgac atcaaccgga gggggatctc cctggtgcgg
     2341 gcttcctcga cgacgtggcg gccgatgaag ccggtcgccc cggtgatcat ggtggtgtgc
     2401 acgcggcact ctccagtcgg ctcgacgagg gcgggcttca gcagtcgcgg ggccgcagga
     2461 cgagctcggc ggagtagacg gcccggccct cctggatgcc ctgcaccgtc actcggacgt
     2521 tgccggaggc gtcgggacgg cccggggccg cctcgatcag gcacggcgtg tcgagttcga
     2581 cgtagcgggt gaagacgctg tcgagggcga cgacgagcgc gtcgggcagt ccggtggagg
     2641 cgtgcgcggc ctgccgggcc gcctccagca gcaccatgcc cgggacgtgg tcgaccgggt
     2701 ggtcgaagaa gatcgggtgc gtggtgtcga cgcgcagttc ccaccggtgc gctcccgcac
     2761 ccgtgccgtc gtcggccagg accacgtgcc cggcgccgtt acgcccgacg cgtgccgggt
     2821 cgatcgggcg gcccggcacg tagccggtgc tggtggggcg gtcgccgcgc agccgccggt
     2881 ggacggcggg gctcgtgcag ctgaagccgg cgcgcccggt ggccagggcc actccgtcga
     2941 cgacggccgt gacgtcgtac cgcatgctgc tgagcgatct tcccctgcgg accacatcgc
     3001 tgcagaccgt gtgcagctcc acctcggtcg gcgcggggcc cgcgacgagc atctcctcgg
     3061 tggtggcgaa cgacatgtcc cacatcagga actggtggcc gaagggcgcc ccgaactcgg
     3121 cgtgggacag caacgatccg atctggcgga ccgactcgat cagcagcatg gggtcctgat
     3181 acccggcgac ctgcgagaag agggagtgcc cacggggcca ctgggcccgt acgacgaacg
     3241 attcggcccc gccggcggcg ctgtcggcga ctgcctccca cccggtgagc agcacctcgg
     3301 agacggaggc ccggtgtacg tactggcgcg gcacggtcgt ggtgagctcg gggatgggcg
     3361 gggcgggacg cggcgttaag acgatgttcc tgctgggctc gtacggtgct gaggcaaggg
     3421 acggcatgcg tggcactccc gatctatgtc tctggacagg ggtgagcaag ccggacgccg
     3481 gcagccaccc ccgtggccga cgattcgttg ctgaccccaa gatacagaca ggtcggttcg
     3541 taattgcaag taaaaacttg gtctgcaccc cgccggactc atatgtaccg agcggccatc
     3601 cggccacccg caagcggaag cacgaccacc cacttcgcct caaagtggac tcctggtgac
     3661 atacacaaca cgtacgccgg acattcagtc acccggaacc cttccggtac ccccacccga
     3721 aaaacaggca agtcagcctg ataacgcctc tgatcaggcg ccttgggtgc acgcggacac
     3781 ccctgaccgg acctctcgaa tcaccgtcca agtcaccaca aattcggcgt catcgcaggt
     3841 cacttcgatg gatacacgag caacacggcc cgccttgtcg tccgttgaca atcagacaag
     3901 tctgtttgct atcttcggta ggcgtgagca ttcagcagtc cgtgatgtca cgcctgtgcc
     3961 tcaggaggag cagatgaacc ggcaggcctc agatctactc gaaccccgga ccactcagca
     4021 tcgccctttg gtttcccagc cccaccgcgc ctcagccctg cctcatccac cctctgccgg
     4081 agagcgtgac gtactgcgtg tcctggtcgt cgagaacgag gaacgtgccg ccgacaccct
     4141 cgtgcaggcc ctgctcagac agggttacga cgccgagagc gtggccaccg gcggcaaggc
     4201 gttacagggc caccaccagg ccgacctgat cctgctcgac ctcgacctcc ccgacctgga
     4261 cggcctggag gtctgccgcg ggatccgggc cgtgagcgac accccgatca tcaccgtgac
     4321 cgcgcgcggc agcgaactcg accgggtgct gggactccag gccggctcgg acgactacat
     4381 ggtcaagccc tacggattcc gtgaactgct ggccaggatc gaagcggtga tgcgccggtc
     4441 gcgtccccgc caggagatcg cgaggaccgt cgaccacggc ccgctccgga tcgacgccac
     4501 cacccgcgag gcgacgctgg acgggcgcca ggtggacctc acccgcaagg agttcgacct
     4561 cctgcacctg ctcgcctccc agccgggggc ggtgatctcc agacgccagc tgatgactca
     4621 ggtctgggag gacacctggt cgcagcgggg ccgcacgatc gacacccatg tcagcagcct
     4681 gcgggggaag ttggggtcga gtagctggat catcaccgtc cggggggtcg gcttccgcct
     4741 cgggcacccc tgagatgccc cggcggggcc ggggcaccgg gggcggcgcg agcgcggacc
     4801 cggcgccctc ggcactcgcg ggaaccacca ctttccggaa ggtgcgcccc caggtaatga
     4861 cgccgtgcgg gcttcgtcat aggatgcaga caagtcggtt tgatatccgt ggtggcgagc
     4921 gcaaggatcc gggatgggta agcaggaacg cgggacaagg tcgcgactct cgatcctgga
     4981 agccgccgcc cgggtgttcg acgcccgcgg cttcgacgcc gcgagcacca acgagatcct
     5041 ggcgagcacc ggcctgacca ggggcgcgct gtaccaccac ttcccctcca aggaagccat
     5101 cgccgtagcg ctgctggccg cgcacgacga ggcactggtc gtcccggacc gccaggtgaa
     5161 gctccagtcg gtcatcgacc tcaccttcga gttcgcgttc cgcctccagc gcgacccggt
     5221 gctgcgggcg agcgtgcgcc tggccgtcga gcagacgtcc ttcccccgcc ccgtgaccac
     5281 gccgtacgag cagtcgggct cggccataaa cgaactgctg aagcaggcac agcagcaggg
     5341 cgagatcctg ccgggcgtgg atctggtgga ggcgacctcg atcatcatcg gcgcgttcac
     5401 cgggatgcag gtgatgtcgc agatgtacac caaccgacgc gatctgccgg agcgcgtcag
     5461 cacgatgtgg cgtttcctgc tgccggggct ggccaacccg ggcatgatca gccacctgcg
     5521 gatcaccccc gcccctgaat cgacaccgaa gccggaaccg actccgaagc cggagttgga
     5581 gcggaccggg gagccggcag ggagtcccgc cc
//
