LOCUS       NZ_CP042828            10393 bp    DNA     circular CON 27-APR-2024
DEFINITION  Ruania zhangjianzhongii strain HY168 chromosome, complete genome.
ACCESSION   NZ_CP042828
VERSION     NZ_CP042828.1
KEYWORDS    RefSeq.
SOURCE      Ruania zhangjianzhongii
  ORGANISM  Ruania zhangjianzhongii
            Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Ruaniaceae;
            Ruania.
REFERENCE   1  (bases 1 to 5067305)
  AUTHORS   Huang,Y.
  TITLE     Ruania sp. HY168
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5067305)
  AUTHORS   Huang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2019) National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, Changping, Beijing 102206, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. HGAP 3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 178.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_008000995.1-RS_2024_04_27
            Annotation Date                   :: 04/27/2024 03:28:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,654
            Genes (coding)                    :: 4,621
            CDSs (with protein)               :: 4,621
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 33
            CDSs (without protein)            :: 33
            Pseudo Genes (ambiguous residues) :: 0 of 33
            Pseudo Genes (frameshifted)       :: 13 of 33
            Pseudo Genes (incomplete)         :: 20 of 33
            Pseudo Genes (internal stop)      :: 1 of 33
            Pseudo Genes (multiple problems)  :: 1 of 33
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:19:52
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1497670
            Orig. end                         :: 1508063
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP042828.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5393)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            795..1391
                     /locus_tag="FU260_RS06920"
     CDS             795..1391
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /locus_tag="FU260_RS06920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="single-stranded DNA-binding protein"
                     /protein_id="WP_147916393.1"
                     /transl_table=11
                     /translation="MVNEIEVTLRGHVGHEPNLLASPGKQTFLRLNVASTPRVRSREGE
                     WGDGATQWFAVKLFGEFAEHVAHSVRKGDAVLVRGRLEHEEYQTRDGEQRWTAVIVADA
                     FGPDLRTATAHVTRAVRRADAETETGSAATEEASEPAGTAADSASEEEADSATEAGEAT
                     ESSEEQDAGVDLSGFQVLPDDDTETEESMAERMSA"
     gene            1487..3169
                     /gene="ettA"
                     /locus_tag="FU260_RS06925"
     CDS             1487..3169
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /gene="ettA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786651.1"
                     /locus_tag="FU260_RS06925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="WP_147916394.1"
                     /transl_table=11
                     /translation="MAEYIYSMVKARKAHGDKVILDDVSMSFLPGAKIGMVGPNGAGKS
                     TILKIMAGLEQPSNGDARLAPGYSVGILLQEPPLNEEKTVLGNVEEGVSQIKGKIDRFN
                     EIGNQMAEPDADFDALMAEMGKLQGEIDAADAWDLDSQLEQAMDALQLPPGDAEVTHLS
                     GGERRRVALAKLLLEQPDLLLLDEPTNHLDAESVQWLEQHLAKYPGAVIAVTHDRYFLD
                     HVAGWIAEVDRGRLYPYEGNYSTYLEKKSARMEIQGKKDAKLAKRLKDELEWVRSSAKG
                     RQTKSRARLARYEEMAAEAERTRKLDFEEIQIPPGPRLGSLVIEAENLRKGFGDRLLID
                     GLSFTLPRNGIVGVIGPNGVGKTTLFKSIVGLEELDGGELKVGDSVKISYVDQGRENID
                     PNKTLWEVVSDGLDFIQVGNVEIPSRAYVSQFGFKGPDQQKPAGVLSGGERNRLNLALT
                     LKQGGNLLLLDEPTNDLDVETLGSLENALLNFPGSAVVISHDRWFLDRVATHILAYEGT
                     EENPANWYWFEGNFADYEENKVERMGPEAARPHRVTYRKLTRD"
     gene            3173..3634
                     /locus_tag="FU260_RS06930"
     CDS             3173..3634
                     /EC_number="3.1.2.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 4HBT_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883124.1"
                     /locus_tag="FU260_RS06930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thioesterase family protein"
                     /protein_id="WP_147916395.1"
                     /sec_met_domain="4HBT_2 (E-value: 2.1e-17, bitscore: 61.7,
                     seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRMRVPVQMRWADLDAYGHVNNAAMLTLLEEARIAVFWTSQGEE
                     SMVSGAKVLSGGPGSDTHTLVARQEIEYLAPLSYSQDPVGVELWIGQIGGASLLVCYEV
                     LTPTGEVCARAASSIVKVDAGTGRPARFSDAEREALAPLVDDPVEFRRR"
     gene            complement(3920..4813)
                     /gene="thpD"
                     /locus_tag="FU260_RS06935"
     CDS             complement(3920..4813)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884777.1"
                     /locus_tag="FU260_RS06935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_147916396.1"
                     /sec_met_domain="PhyH (E-value: 1.6e-30, bitscore: 105.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAATARTDAYPTRVSTEDPIAREHPTVWGSASDGPFDADTLRTH
                     EERGFTICEDFLSAEEVETFGAELTRLTSDATLRSDERVITEKASGQVRSLFEVQDFSA
                     PIDALSRDPRLLDRARQLLGSEVYLHQTRVNYMPGFQGTGFYWHSDFETWHAEDGMPLP
                     RAVSCSIALTPNYPFNGGLMVIPGSHRTFIPGVGVTPEDNYASSLKEQTVGVPSEAAIT
                     RMAAEHGIDQFTGPAGSALWFDANIMHGSGNNITPFPRSNIFLVFNSVENALEEPYAAP
                     APRPGYIGNRDVTPLR"
     gene            complement(5001..5393)
                     /locus_tag="FU260_RS06940"
     CDS             complement(5001..5393)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776472.1"
                     /locus_tag="FU260_RS06940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_147916397.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.2e-53, bitscore:
                     176.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRTLDDVTDTEDDIKTENWRSKRIILAREGVGFSVHETTLYAG
                     TDNYFWYANHIEAVFITHGEGEIEDLATGEVHQLAPGSLYLLNNHDKHRVRPRTEIRCV
                     CVFNPPVTGREVHDENGVYPLIVEEA"
     gene            complement(5433..6695)
                     /gene="ectB"
                     /locus_tag="FU260_RS06945"
     CDS             complement(5433..6695)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (25-362). E-value: 5.4e-70.
                     Score: 228.0. Matches aSDomain:
                     nrpspksdomains_FU260_RS06945_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512924.1"
                     /locus_tag="FU260_RS06945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_147916398.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5e-75, bitscore:
                     250.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTSIDATALESGVRSYSRTWPTTFTKAQGSFMYNEEGDQYLDF
                     FAGAGVMNYGHNNRHLKEVLIKYLAGDNVVHSLDMLTTARTDFLSTFTDQILRPRGLDH
                     TVMFPGPGGANAVEAALKLARKVTGRESVVTFTNAFHGMTLGALSVTGNSMKRGGAGIP
                     LVHATPMPYDDYFDGDVPDFMYLERLLNDAGSGLNTPAAVIVETVQGEGGVNTARREWL
                     RQLSSLCKEQGIILIVDDIQMGCGRTGGFFSFEEAGIVPDIICLSKSISGYGLPLALTL
                     LRPELDVWEPGEHNGTFRGFAPAFVTGAEAIRTYWSDDDLQRSTIAKGAFAESALNTIV
                     ADHPEHGLSAKGRGLARGLEFTNPDHAAATCRNAFEAGLLVETSGPRDEVIKLLPPLTL
                     TNEELARGLDILRQATADAVA"
     aSDomain        complement(5610..6620)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FU260_RS06945_Aminotran_3.1"
                     /evalue="5.40E-70"
                     /label="FU260_RS06945_Aminotran_3.1"
                     /locus_tag="FU260_RS06945"
                     /protein_end="362"
                     /protein_start="25"
                     /score="228.0"
                     /tool="antismash"
                     /translation="FTKAQGSFMYNEEGDQYLDFFAGAGVMNYGHNNRHLKEVLIKYLA
                     GDNVVHSLDMLTTARTDFLSTFTDQILRPRGLDHTVMFPGPGGANAVEAALKLARKVTG
                     RESVVTFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDDYFDGDVPDFMYLER
                     LLNDAGSGLNTPAAVIVETVQGEGGVNTARREWLRQLSSLCKEQGIILIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIICLSKSISGYGLPLALTLLRPELDVWEPGEHNGTFRGFAPAFV
                     TGAEAIRTYWSDDDLQRSTIAKGAFAESALNTIVADHPEHGLSAKGRGLARGLEFT"
     misc_feature    complement(6675..6689)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     gene            complement(6813..7385)
                     /gene="ectA"
                     /locus_tag="FU260_RS06950"
     CDS             complement(6813..7385)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015776470.1"
                     /locus_tag="FU260_RS06950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_147916399.1"
                     /transl_table=11
                     /translation="MPLPTESDTRPGTPEPSATTDILSTTPTASDEPLQIRPPEAGEGA
                     AMWRLARDGGELDLNTSYAYLILADDFAATCRVAVSGGEVVGFVLGYCPPARPSHVFVW
                     QVAVRSDQRGRRVAARMLDDLVDGLPEVEAMEATVTESNTASRRLFAAFARNRGAELSW
                     SAGVRAEDFPDGHEAEPRLLISPLSRS"
     gene            7591..8592
                     /locus_tag="FU260_RS06955"
     CDS             7591..8592
                     /EC_number="4.2.1.126"
                     /GO_function="GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /GO_function="GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017824379.1"
                     /locus_tag="FU260_RS06955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="WP_147916400.1"
                     /transl_table=11
                     /translation="MSVTLGGYAGEVELEGAAEGKPVLAVQVRSPTEERNPRTTDIDTL
                     DASGVVVAILTEDASVAAAVAARSAAITVLVERGVAALADGGRVVYVGAGTSGRLAVLD
                     AAELLPTYQVSEDQVRAHIAGGAPAMLHPVEGAEDDPELGRVAVADVTARDLVLGLAAS
                     GRTPYVAGALRHARSVGASTGLVSCNPHAPLAELADVAVLVDTGPEVVTGSTRMKAGTA
                     QKMVLNTFSTAVMVRLGKTYSNLMIDVLPTNEKLHGRVLRMLTQASGADLHRCQEALEA
                     SGSPRVALVSLLADVPPQAAAQVLAAHPPDDRRAGDPSGVRSAVEQLRVSRR"
     gene            complement(8685..9560)
                     /locus_tag="FU260_RS06960"
     CDS             complement(8685..9560)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770552.1"
                     /locus_tag="FU260_RS06960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl-CoA thioesterase II"
                     /protein_id="WP_147916401.1"
                     /transl_table=11
                     /translation="MTGGQEVDPLQAVLAALVLEQAGEDTFVGTSLPQIHGRVYGGQVL
                     AQALLAAGRTMTTDRLPHSVHGYFLRPGDLDEPITFAVERLRDGRSFSARRTHAIQSGK
                     PILSMITSFQVEQEGLDHAEPMPQVPAPEELDSSVEFFNSLDHPIAKFFYRNGAFDLRH
                     VDGALFTSPGEVRTDRQYLWMRARGPLPVGQLEQRAILAYACDQVMLEPIMRRHGLSWM
                     TKDASVASLDHAMWWHRNVDVGDWLLYAQSSPSAGGGRGLGEAKVYARSGELVATIAQE
                     GMIRIPQIPS"
     gene            complement(9557..9970)
                     /locus_tag="FU260_RS06965"
     CDS             complement(9557..9970)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883122.1"
                     /locus_tag="FU260_RS06965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="globin"
                     /protein_id="WP_280527391.1"
                     /transl_table=11
                     /translation="MSVTPARAERPDSFYAAVGGEPTFRFIVHRFYQQVATDDLLRPMY
                     PEEDLGPAEDRLRMFLEQYWGGPTTYSETRGHPRLRMRHVPFRVTPTARDRWLQFMTVA
                     VQEAELAPMHEARLLDYLERAAHSMVNTLEEDA"
ORIGIN
        1 accgctcggg acgaggtggc agcggtacgc cgacggctct ccgggcacgt gggggccgcg
       61 gatccggcac cgccggaggc tgcgcaggtg gccgaggatc tggcgacggc gtccggggtg
      121 ccgtcggtgg cggagtcgat ccgccgggcg gtggcctcac cgcgcccagt ggcgctgagt
      181 gctgtgcaac gtccggcagg cagccggacc gcggcgatcc gggaccggtg gctggcagca
      241 gcgactgccg gtctgcctgc gccgtggtcc gacgcggtcc aggcccaggt agccaccgag
      301 gagtcgttcg acgaccacgt gtggacggca ctcgaaggac agcagctgcc ccgcagcacc
      361 gaccgggtgg ccagcgggca gcggttggtc ggcttcgtag ccggcgggct cgggctggca
      421 ctgctgatcg ccggcgccgc gctcttcgcc gggtccgccg tggttggtgg ctcattggcc
      481 ggggcggggg tggcgctgct gctcgcctgc ctcgtgctga tcgtcgtggc cggcaggcgc
      541 aggcgccgca cctccgccga acgcgctgag ggctatatgc agagcacgac ggcggagctc
      601 gcctctgtgg tctcagcgga cctggtccgg cctgctcagg cacctctgga ccagcaccgc
      661 acggcgcgtg ccggcatcca cggcgatgcc acagtttcca cagcccgggg gtgaggccca
      721 cccgcggtgt gctcagttag gtgaaggtga ctctatgcac cggacaggcc ggtgcctgag
      781 caactggagt caccatggtc aacgagatcg aggtcaccct tcggggccac gtggggcacg
      841 agccgaacct gctggcgagt ccgggaaagc agacgttcct gcggttgaac gtcgccagta
      901 cgccgcgggt ccgcagccgc gagggggagt ggggtgacgg cgccactcaa tggtttgcgg
      961 tgaagctctt cggggagttc gccgagcatg tggcgcacag tgtgcgcaaa ggagatgcgg
     1021 tgctggtccg cggccggttg gagcatgagg agtaccagac ccgcgacggt gagcagcgct
     1081 ggactgccgt gatcgtggcc gacgcattcg gcccggatct gcgcacggcc acggcgcacg
     1141 tcacccgcgc ggttcggcgc gccgacgccg agaccgagac cggctcggcg gctaccgagg
     1201 aggcgagcga gcccgccgga actgctgccg actctgcgtc ggaggaggag gcggattcgg
     1261 ccacggaggc gggcgaggct accgagagca gcgaggagca ggacgccggg gtggacctat
     1321 ccggattcca ggtgctgccc gatgacgaca ccgagacgga ggagtcgatg gcggagcgga
     1381 tgagcgcctg acctgcgccc gggctccgat tgccatgatc ggggggcgac gggcgtagag
     1441 tgaggggttg atctaaccct gaaacctgct catcgctggg agaaaagtgg ccgagtacat
     1501 ctactcgatg gtgaaggcgc gcaaggcgca tggcgacaag gtcatcctcg atgacgtgtc
     1561 gatgtcgttc ctgcccggag cgaagatcgg catggtcggc ccgaacggtg ccggaaagtc
     1621 gacgatcctg aagatcatgg ctggactgga acaaccctcc aacggtgacg cccggctggc
     1681 cccgggctac tccgtgggca tcctcctgca ggagccgccg ctgaacgagg agaagaccgt
     1741 tctcggcaac gtcgaagaag gcgtcagcca gatcaagggc aagatcgacc ggttcaacga
     1801 gatcggcaac cagatggccg agccggacgc cgacttcgat gcgctgatgg cggagatggg
     1861 caagctccag ggcgagatcg acgccgccga cgcctgggac ctggactccc agctcgagca
     1921 ggcgatggat gcgctgcagc tgccgccggg ggacgcggag gtcacccacc tctccggtgg
     1981 tgagcgccgt cgagtggcgt tggccaaact gctcctggag cagcccgatc tgctcctcct
     2041 cgacgagccg acgaaccacc tggacgcgga gagcgtgcag tggctcgaac agcacctggc
     2101 gaagtacccg ggcgccgtca tcgctgtcac ccacgaccgg tacttcctcg accatgtggc
     2161 cggctggatc gctgaggtgg accggggccg gctgtacccc tacgagggca actactccac
     2221 ctacctggag aagaagtccg cccggatgga gatccagggc aagaaggacg ccaagctggc
     2281 caagcgtctt aaggacgagc tggagtgggt gcgctcctcc gcgaagggtc gccagaccaa
     2341 gtccagggcg cggttggccc ggtacgaaga gatggcggcc gaggcggagc gcacccggaa
     2401 gctcgacttc gaggagatcc agatcccgcc cggcccgcgc ctgggctcgc tggtgatcga
     2461 ggcggagaac ctgaggaagg gcttcggcga ccgcctgctg atcgacgggc tgagcttcac
     2521 cttgccgcgg aacggcatcg tcggtgtgat cggcccgaac ggtgtgggca agaccacctt
     2581 gttcaagtcg atcgtcgggc tcgaagagct ggacggcggc gagctgaagg tcggcgacag
     2641 cgtcaagatc tcctatgtgg accaaggtcg ggagaacatc gacccgaaca agaccctctg
     2701 ggaagtggtc tccgacgggc tggacttcat ccaggtgggc aacgtggaga tcccctcgcg
     2761 ggcgtacgtc tcccagttcg ggttcaaggg cccggaccag cagaagccgg ccggagtgct
     2821 ctccggcggt gagcgcaacc ggctgaacct ggcgctgacc ctcaagcagg gcgggaacct
     2881 gctgctgctg gacgagccca ccaacgacct ggacgtggag accctgggca gcctggagaa
     2941 cgcactgctg aacttcccgg gttcagccgt ggtgatctcc cacgaccggt ggttcctgga
     3001 ccgtgtggcc acgcacatcc tcgcctatga gggcaccgag gagaacccgg cgaactggta
     3061 ctggttcgag ggcaacttcg ctgactatga ggagaacaag gtcgagcgga tggggccgga
     3121 ggctgcccgt ccgcaccggg tcacctaccg caagctcacc cgcgactgag ctatgggccg
     3181 catgcgggtg ccggtgcaga tgcgctgggc ggacctggac gcctacgggc atgtgaacaa
     3241 tgccgcgatg ctcactcttc tggaggaggc gcgcatcgcg gtgttctgga ccagtcaggg
     3301 cgaggagtcg atggtctccg gcgccaaggt gctctctggc ggcccgggca gcgatacgca
     3361 caccctggtg gcgcgtcagg agatcgagta cctggcgccg ctgtcctaca gccaggaccc
     3421 ggtgggcgtg gagctctgga tcgggcagat cggcggagca agcctgctgg tctgctacga
     3481 ggtgctcact cccaccggtg aggtctgtgc ccgggccgcg agctcgatcg tgaaagtcga
     3541 cgccggtacc ggccgccccg cccggttcag cgatgccgaa cgggaggcgc tggccccact
     3601 ggtggacgat ccggtggagt tccggcggcg gtagcctgac cgaccccacc gcgacctcgc
     3661 gccgggagcg acctggcccg gcagcgaccc ggcccggcag cgacccggcc tcgcagcgac
     3721 ctcgcgcggg tagcgacctc accctcggaa tccacttggt ccctcggaat ccgcttcctc
     3781 ctggattcgg agggggcaag tggatttcca gggccgggca ggtcccggga ccgaacgctc
     3841 cctggtgcgg cgacctcgta cggcctgccg cacgagggag cattcgccct cgctagctcg
     3901 gctggctagc tcgggtccgc tagcgcagcg gggtgacgtc ccggttgccg atgtagccgg
     3961 gtcgcggtgc cggggcggcg tacggctcct ccagcgcgtt ctccacgctg ttgaacacca
     4021 ggaagatgtt cgaccggggg aacggagtga tgttgttccc ggacccgtgc atgatgttcg
     4081 catcgaacca cagcgccgaa cctgccggac cggtgaactg gtcgatcccg tgctcggcgg
     4141 ccatccgggt gatcgcggcc tcgctgggca cccccacggt ctgttccttc agcgaggagg
     4201 cgtagttgtc ctccggggtg acgcccacac cggggatgaa cgtgcggtgc gagcccggga
     4261 tgaccatcag gccgccgttg aacggatagt tcggcgtcag ggcgatcgag cagctcaccg
     4321 cccggggcag cggcataccg tcctcggcgt gccaggtctc gaagtcggag tgccagtaga
     4381 agccggtccc ctggaaaccc ggcatgtagt tcacccgggt ctggtgcagg tacacctcgg
     4441 acccgagcaa ctggcgcgcc cggtccagca gccgagggtc gcggctgagc gcatcgatcg
     4501 gcgcgctgaa gtcctggacc tcgaagaggg agcggacctg gccggaggcc ttctcggtga
     4561 tcacgcgctc gtcgctgcgc agtgtggcgt cagaggtgag ccgggtcagc tcggcgccga
     4621 acgtctccac ctcctcggcg ctgaggaaat cctcacagat ggtgaagccg cgttcttcgt
     4681 gagtcctcag tgtgtcagcg tcgaacggac cgtccgaggc cgaaccccac acagtggggt
     4741 gctcgcgggc gatcggatcc tcagtgctca cgcgcgtggg gtaagcgtcc gtccgtgcgg
     4801 tagcggctgt catcgattct cctttcgtcg gcgaaaacgc cgcacccggt gcggcagttg
     4861 gccgcaccgg gtgctagatg acgacaccgt ggtgccgcca ggtccttcca gcgcaaacgc
     4921 cctcccgcgc ggtgcagatc atcagatctg ctcgcgcgga agagcgttcg cgaggtacta
     4981 ctgaggggcc gcgttgcgaa tcaggcctct tcgacgatca acgggtacac gccattctcg
     5041 tcgtgcacct cccggccggt caccggcggg ttgaacacgc acacacaccg gatctcggtg
     5101 cgtgggcgca cccggtgctt gtcgtggttg ttcagcaggt acagcgagcc cggcgccaac
     5161 tggtgcacct caccggtagc caggtcctcg atctcgccct caccatgggt gatgaagacg
     5221 gcctcgatgt ggttggcgta ccagaagtag ttgtcggtac cggcgtacag agtcgtctcg
     5281 tgtacggaga agcccacacc ctcgcgggcg aggatgatgc gcttgctgcg ccagttctcg
     5341 gtcttgatgt catcctcggt gtcggtgacg tcgtcgaggg tgcgaacgag catgaacggg
     5401 tccttatctc tggtgacggt tgtcgatgct gctcaggcta cggcgtccgc cgtggcctgg
     5461 cgcaggatat cgagaccgcg agccagctcc tcgttcgtca gcgtcagcgg cggcagcagc
     5521 ttgatcacct cgtcgcgcgg accggaggtc tccaccagca gaccggcctc gaaggcgttg
     5581 cggcaggtgg cggcagcgtg gtccgggttg gtgaactcga gcccacgggc caggccgcga
     5641 cccttggcgg acaggccgtg ctccgggtgg tcggcgacga tcgtgttcag cgccgactcc
     5701 gcgaacgcac ccttggcgat cgtggagcgc tgcaggtcgt catcggacca gtaggtgcgg
     5761 atcgcctcgg ctccggtgac gaaggccggt gcgaacccgc ggaaggtgcc gttgtgctca
     5821 cccggctccc acacgtccag ctcgggacgc agcagggtca gcgcgagcgg caggccgtag
     5881 ccgctgatcg acttggacag gcagatgatg tccgggacga tcccggcctc ttcgaagctg
     5941 aagaagccgc cggtgcggcc gcagcccatc tggatgtcgt cgacgatcag gatgatgccc
     6001 tgctccttgc acagcgagga gagctgacgc agccactccc gccgggcggt gttcacgccg
     6061 ccttcgccct gcacggtctc cacgatcacg gcagccgggg tgttcaggcc cgagccggca
     6121 tcgttgagca accgctcgag gtacatgaag tccggcacgt caccgtcgaa gtagtcgtcg
     6181 tacggcatcg gcgtggcgtg caccagcggg ataccggcgc cgccgcgctt catcgagttc
     6241 ccggtcaccg acagggcgcc cagggtcatc ccgtggaagg cgttggtgaa cgtcaccacc
     6301 gactcccggc cggtcacctt gcgggcgagc ttcagcgcgg cctcgacggc gttggcgcca
     6361 cccggcccgg ggaacatcac cgtgtggtcc aggccgcgtg ggcgcagaat ctggtcggtg
     6421 aaggtggaga ggaagtcggt gcgggccgtg gtgagcatgt ccagggagtg caccacgttg
     6481 tcaccggcga ggtacttgat cagtacctcc ttcaggtggc ggttgttgtg gccgtagttc
     6541 atcacgccgg caccggcgaa gaagtccagg tactggtcgc cttcctcgtt gtacatgaag
     6601 ctgccctggg ccttggtgaa cgtggtcggc caggtgcggg agtagctacg gacaccagac
     6661 tcaagagcag tggcatcgat actggtgagt gtcatgtgag gtcgctttcc ttcgtgaggt
     6721 tgcggatcag gtcgggtcac gcggtgcgag ccgcgcgcag cacgccgagg ccgggaaggc
     6781 cggcgcagcc tgtggctatc aggtgcgtgg aatcagctcc tcgagagcgg actgatcagg
     6841 aggcggggtt ccgcctcgtg cccatcgggg aagtcttcgg cccgcacacc cgcggaccag
     6901 ctgagctcgg cgccccggtt gcgagcgaag gcggcgaaca atcgtcggga ggcggtgttg
     6961 gactcggtca cggtcgcttc catcgcctcg acctcgggca ggccgtcgac gagatcgtcg
     7021 agcatccggg cagcgacccg ccgaccgcgc tggtcgctgc gcaccgccac ctgccagacg
     7081 aacacgtgac tcgggcgggc cgggggacag tagccgagca cgaagccgac cacctcaccg
     7141 ccgctgacgg cgacgcggca ggtggccgcg aagtcgtcgg caaggatcaa gtaggcgtag
     7201 gaagtgttca ggtccagttc gccgccgtcg cgggcgaggc gccacatcgc ggcgccttcg
     7261 ccggcctcag gcgggcggat ctgcagcggc tcgtcggacg ccgtcggggt ggtgctgagg
     7321 atgtcggtgg tagctgaggg ttcaggagta ccagggcgcg tatcggactc agtcggtagt
     7381 ggcatacggc ttctacggta ggcagtcatt gacgagattg aaaccggtcc ttgaccggaa
     7441 tcgggcgatt cccgccgaca gcgggcatgg agatactcgc tcgctgctgc gggaaacctg
     7501 ggggcaccgg acggccggaa aactccctcc agattgagtc catatagtga gacggcccgg
     7561 gaggtcggaa cccgatccct cgatatcgag atgtcggtca cactcggtgg ctacgctggt
     7621 gaggtggagc tcgagggtgc ggcggagggt aagcccgtcc tggccgtcca ggtccgctca
     7681 cccaccgagg agcgcaaccc gcgcactacc gatatcgaca ccctggacgc cagcggtgtg
     7741 gtggtggcga tcctcaccga ggacgcgtca gtggcggctg ccgtggccgc ccggtccgcg
     7801 gcgatcactg tgctggtcga gcgcggggtg gccgcgctgg ccgacggtgg ccgagtggtc
     7861 tacgtcggtg ccggtacctc cggtcggctg gcagtgctcg acgccgcgga gctgctgccc
     7921 acctaccagg tgagcgagga ccaggtccgg gcgcacatcg ccgggggagc gccggcgatg
     7981 ctgcacccgg tggagggtgc cgaggatgat ccggagctgg gccgggtggc agtagcggac
     8041 gtgaccgcac gggatctggt cctcggactt gccgccagtg ggcgcacccc ctacgtggcc
     8101 ggcgcgctgc ggcacgcccg gtctgtcggt gccagcaccg ggctggtctc ctgcaatccg
     8161 cacgccccgc tggccgagct ggcggatgtg gccgttctgg tggacaccgg gccagaggtg
     8221 gtgaccggga gcacgcggat gaaggccggc acggcccaga aaatggtcct gaacaccttc
     8281 tccaccgcag tgatggtgcg gttgggcaag acctactcga acctgatgat cgatgttctg
     8341 ccgacgaacg agaagctgca cgggcgggtg ctgcggatgc tcacccaggc cagcggtgcg
     8401 gatctgcacc ggtgccagga ggcgctcgag gcgtccggca gcccgcgggt agcgctggtc
     8461 agtctgctgg ccgacgttcc accgcaggca gcggcgcagg tgctggctgc ccacccaccc
     8521 gatgaccggc gtgcggggga tccctcgggg gtgcgatccg ccgtcgagca gctccgcgtg
     8581 tcgcgtcgct gaggtgcgtc gctccgcctg tccagccgct gaggtgcgtc gctccgcctg
     8641 tccagccgct gaggtgcgtc gctccgcccg gtgaggcgtg cggctcagct ggggatctgc
     8701 ggaatccgga tcatgccctc ctgggcgatc gtcgctacca gctcaccgga gcgggcgtag
     8761 accttggcct cgcccagccc gcggccgcct ccagccgacg gggaggactg ggcgtagagc
     8821 agccagtcgc ccacgtccac gttccgatgc caccacatcg cgtggtccag gcttgccact
     8881 gaggcgtcct tggtcatcca ggacagtccg tgccggcgca tgatcggctc cagcatcacc
     8941 tggtcgcagg cgtaggcgag gatcgcgcgc tgctccagct gcccaaccgg caacggtcca
     9001 cgtgcgcgca tccacaggta ctgccggtcg gtgcggacct cgccagggct ggtgaacagg
     9061 gcgccgtcga cgtgccgcag atcgaacgcg ccgttgcggt agaagaactt cgcgatcggg
     9121 tggtccaggg aattgaagaa ctccaccgag ctgtccagct cctccggggc cggaacctgc
     9181 ggcatcggct cggcgtggtc cagaccctcc tgctccacct ggaacgaggt gatcatcgac
     9241 aggatcggct tgccggactg gatcgcgtgc gtgcgcctgg cggagaacga gcggccgtcc
     9301 cggaggcgtt cgacggcgaa ggtgatcggc tcgtccaggt caccggggcg caggaagtag
     9361 ccatgcacgg agtgcggcaa cctgtccgtg gtcatcgtgc gcccggcggc gagcagcgcc
     9421 tgggcgagca cctggccgcc gtagaccctg ccgtggatct gcggcagcga cgtgcccacg
     9481 aacgtgtcct cgccggcctg ctccagcacc agggcggcca gcaccgcctg cagcgggtcg
     9541 acctcctgcc cgccggtcat gcgtcctcct ccagggtgtt caccatcgag tgcgcggccc
     9601 gctccaggta gtccagcagc ctcgcctcgt gcatcggggc cagctcggcc tcctggaccg
     9661 ccacggtcat gaactgcaac cagcggtccc gagcggtcgg ggtgacgcgg aacggtacgt
     9721 gccgcatccg cagtcgcggg tggccgcggg tctcggagta ggtggtcggg ccaccccagt
     9781 actgctccag gaacatccgc agccggtcct cggccggtcc gagatcctcc tccgggtaca
     9841 tcggccggag caggtcgtca gtggctacct gctggtagaa ccgatgcacg atgaagcgga
     9901 acgtcggctc gccaccgaca gcggcgtaga aggagtccgg gcgttcggcc cgcgcgggtg
     9961 tcacactcag gccgggccga gcggctgggc gggggctggg atctggcagg ctcacgggtt
    10021 cagcctaagc ggttctctcg gtgggttcgg cgttgctcgt cggccggctg gccgcggtcc
    10081 ggacgtcaca gggcaagctc ggccagggcg gggagcgcgc cgcgggccgc gcggcgggcg
    10141 tcctcggcgc tgtcggcggc gagtgccaga ctcgctgccg tgcggcactg cgcgagcgtg
    10201 gtggccgcca gcagggcgcc gacgtcgccg agcgcccgcg gcgtcatcga cagactggac
    10261 acccccatac cgaccaacac cgctgccagg gcgggatcgg cggccgcctc cccgcagacc
    10321 cccaccggac gggactgggc tgctccgccg tcgcaggtgc gctggagcag ctgcagcacc
    10381 gcggggtccc acg
//
