LOCUS       NZ_VOGX01000074         8672 bp    DNA     linear   CON 15-OCT-2024
DEFINITION  Streptomyces albidoflavus strain 77 scaffold34_cov50_4369_13040,
            whole genome shotgun sequence.
ACCESSION   NZ_VOGX01000074
VERSION     NZ_VOGX01000074.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces albidoflavus
  ORGANISM  Streptomyces albidoflavus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces albidoflavus group.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Pylro,V., Dias,A., Andreote,F., Varani,A., Andreote,C., Bernardo,E.
            and Martins,T.
  TITLE     Draft Genomic Sequences of Streptomyces misionensis and Streptomyces
            albidoflavus, bacteria applied for phytopathogen biocontrol
  JOURNAL   Microbiol Resour Announc (2019) In press
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   Pylro,V., Dias,A., Andreote,F., Varani,A., Andreote,C., Bernardo,E.
            and Martins,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2019) Biology, UFLA, Universidade Federal de
            Lavras, Lavras, MG 37200000, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-JUL-2019
            Assembly Method                   :: SPAdes v. 3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 36.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007896905.1-RS_2024_10_13
            Annotation Date                   :: 10/13/2024 04:20:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,551
            CDSs (total)                      :: 6,456
            Genes (coding)                    :: 6,343
            CDSs (with protein)               :: 6,343
            Genes (RNA)                       :: 95
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 5 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 34 of 113
            Pseudo Genes (incomplete)         :: 81 of 113
            Pseudo Genes (internal stop)      :: 8 of 113
            Pseudo Genes (multiple problems)  :: 8 of 113
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:41:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8672
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VOGX01000074.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8672
                     /collection_date="2019-07-15"
                     /db_xref="taxon:1886"
                     /geo_loc_name="Brazil"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces albidoflavus"
                     /strain="77"
                     /submitter_seqid="scaffold34_cov50_4369_13040"
     region          1..8672
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..8672
                     /SMILES="NC(CCC(=O)N)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8672
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[3351:7182](+)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3352..7182
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     misc_feature    complement(302..313)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(388..401)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     gene            728..1372
                     /gene="upp"
                     /locus_tag="FRZ02_RS30990"
                     /old_locus_tag="FRZ02_30990"
     CDS             728..1372
                     /EC_number="2.4.2.9"
                     /GO_function="GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="upp"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471090.1"
                     /locus_tag="FRZ02_RS30990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_30990"
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="WP_093846127.1"
                     /transl_table=11
                     /translation="MLLLPQTNQLRALHTVIRDRSASGRDFEVYSRRIIRLLLEAGLDL
                     LPFGKRDVTTPVGETYHGLEFTPQVCGVSVVRAGESMEAELRDMIPGIPIGKILIQRDK
                     RTKQPELFYKHLPGDIGSGHVLLLEPMLATGGSALAALDVLRDAGVPEENVVLVNFLAS
                     PTGLERVSLAAPEMKIVTSSVEDGMNEHAFMVPGIGDFGDRFFGTVPTAAS"
     misc_feature    755..757
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1369..2412
                     /locus_tag="FRZ02_RS30995"
                     /old_locus_tag="FRZ02_30995"
     CDS             1369..2412
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471089.1"
                     /locus_tag="FRZ02_RS30995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_30995"
                     /product="EamA family transporter"
                     /protein_id="WP_129846430.1"
                     /transl_table=11
                     /translation="MRQRAGFDSALTVLAPVVWGSTYLVTTQFLPPDRPLLAAAVRALP
                     GGLILTLAGRKLPQGHWWWRILALGALNIGFFFYLLFVAAYHLPGGVAALVGSIQPVFV
                     LLLSALLLREKIRSWQVGACVLGTAGVGMLVLGPEAGLDAVGVAAGLGGAACMAAGIVL
                     TKRWGRPPGVGLLTFTGWQLAVGGLLIAPVALAAEGLPDRLTWPNFAGFAYLSLIGALL
                     AYAVWFRGIERLPALTVSLLGFASPLAATVLGYLFLRQGLTLVQGAGAVAVISAVLLAQ
                     RSGARESRAARKSGPPPEPAEAGTETPAAAGPAHPAPPSPQPGDRSAARSSQPIPESRK
                     ITEKEQS"
     gene            2409..3071
                     /locus_tag="FRZ02_RS31000"
                     /old_locus_tag="FRZ02_31000"
     CDS             2409..3071
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003946753.1"
                     /locus_tag="FRZ02_RS31000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_31000"
                     /product="HAD family hydrolase"
                     /protein_id="WP_121842212.1"
                     /sec_met_domain="HAD_2 (E-value: 2e-43, bitscore: 137.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRPVLFDLDGTLVDTPNAIVENFTATLAELGAPAASPEEIRSTI
                     GLPLEKAFAQLIGASVPAATLDAAVPCYQRLYREQIVPKAKSLLYPGVVDGLAELSEAG
                     LLLAIATSKHTASAEVLLTAAGIRDRFAVVVGADAVTEPKPAPETGLLIAAELGHPAAR
                     AIMVGDTTHDISMAHNAGMRSVAVTYGIHSPGQLAAVRPTWTVRDFPTAVERILRAD"
     gene            3352..7182
                     /locus_tag="FRZ02_RS31005"
                     /old_locus_tag="FRZ02_31005"
     CDS             3352..7182
                     /NRPS_PKS="Domain: AMP-binding (20-420). E-value: 3.2e-106.
                     Score: 347.3. Matches aSDomain:
                     nrpspksdomains_FRZ02_RS31005_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (934-1003). E-value: 1.3e-19. Score:
                     62.2. Matches aSDomain: nrpspksdomains_FRZ02_RS31005_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (1023-1266). E-value:
                     7.6e-25. Score: 80.6. Matches aSDomain:
                     nrpspksdomains_FRZ02_RS31005_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508254.1"
                     /locus_tag="FRZ02_RS31005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_31005"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_146583356.1"
                     /sec_met_domain="AMP-binding (E-value: 1.4e-113, bitscore:
                     368.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 9.3e-15, bitscore:
                     44.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Thioesterase (E-value: 2.7e-22, bitscore:
                     68.9, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSTPPPATRQHASLPALLRHQARERGDDTAVTAAEGRLTFAEL
                     HSAATRMGSHLVGAGVSPDTCVGLFVEPSADLVTGVWGILTAGAAYLPLSPDYPDSRLR
                     HMAADAGVGVVVTQSHLRARLHGLVPAGTLVVTVDEATDADGAHPLPEPREDHLAYVIH
                     TSGSTGSPKGVMIEHRSVVAQLRWLTRRGYLGPAVSVLQKTPISFDAAQWEILACAAGA
                     RVVMSPPGSFRDPQAVIDAIHTYGVTCLQGVPTLLRALVDTGELETCSTLTRIFSGGEA
                     LTRALAQELLRTLPGVSLVNLYGPTETTINATSLEVSPDTLDDEDLHILPIGRPADGVT
                     CHILDENLRPVGTGRSGELFIGGVQVARGYAGRPEATRERFLTAPSAPGERLYRTGDLA
                     RWRPDGTIQYEGRTDNQVKLRGYRVELEEVASRVEEHMWVRKAAAVVTDNPRTGSQVLV
                     AAVELNEHTAAVMDQGRSDAGHHQSKSSKLQVKAQLANPGLREPEELAGRTVVPLAGAE
                     AMPEQRRAAFARKSYRYYEGGRATAEQLTALLTSPPPPAAAARRPGGLTPQELGSLLRW
                     LGPHRSPDRLLPKYAYASPGALYATQLYLEIDGVAGLSGVYYHHPFDHTLVRVGDHPAP
                     GRTAELKIHFLGKRRAVEPVYKNNIVEVLEFEAGHMLGVLEEVLPGMGLMVAPHGFDPD
                     AAARLDVAADDHYLGTFAVVPHDGRPRPEEVELFLQVHGDRVEGLPGGLYRHQDGTLVR
                     LGDEVVEQRHVIAINQGVYERASFGIGAVARTSDDWRRYMVLGTLLHRLQRVGGMGLMS
                     AGYSSRTGHPLPAAQRLDDLLSRASVRAAPASYFFVGGPVSAEQAAHEGMNEDAVHTKG
                     PAEMIRDDAAQFLPDYMVPARVVVVDQLPVTPNGKTDLKATALLPEVAAGRNTTPHVAP
                     ATPTERWLAGEWGRLLGYEDVSTRDEFFASGGNSLHSVALVNRINRRFGTQFPLQTVFE
                     APTLAELAARVAERAPAPASRLIRLNSDGAPARAFVWPGLGGYPMNLRPLALGFDGERS
                     VTAVQAQGINTGETPHPTVRRMAEADIAEIRRVQPSGPYTLWGYSFGARVAFEAAWQLE
                     QAGEKVEDLLLICPGNPRVRTEDGRRRGREASFANPVYVTILHSVFTGTTHGPAVAACL
                     RETTDEDSFVAFVHRTTPALSPETIRRITQVVAATYAFEYTFSELRERRVEAPVTVFKA
                     HGDDYSFLEDSSGYSARPPRVVPLTGDHYEVLRERGVAELISGIRALDLFA"
     aSDomain        3412..4611
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FRZ02_RS31005_AMP-binding.1"
                     /evalue="3.20E-106"
                     /label="FRZ02_RS31005_AMP-binding.1"
                     /locus_tag="FRZ02_RS31005"
                     /protein_end="420"
                     /protein_start="20"
                     /score="347.3"
                     /specificity="substrate consensus: Gln"
                     /tool="antismash"
                     /translation="RHQARERGDDTAVTAAEGRLTFAELHSAATRMGSHLVGAGVSPDT
                     CVGLFVEPSADLVTGVWGILTAGAAYLPLSPDYPDSRLRHMAADAGVGVVVTQSHLRAR
                     LHGLVPAGTLVVTVDEATDADGAHPLPEPREDHLAYVIHTSGSTGSPKGVMIEHRSVVA
                     QLRWLTRRGYLGPAVSVLQKTPISFDAAQWEILACAAGARVVMSPPGSFRDPQAVIDAI
                     HTYGVTCLQGVPTLLRALVDTGELETCSTLTRIFSGGEALTRALAQELLRTLPGVSLVN
                     LYGPTETTINATSLEVSPDTLDDEDLHILPIGRPADGVTCHILDENLRPVGTGRSGELF
                     IGGVQVARGYAGRPEATRERFLTAPSAPGERLYRTGDLARWRPDGTIQYEGRTDNQVKL
                     R"
     aSModule        3412..7149
                     /complete
                     /domains="nrpspksdomains_FRZ02_RS31005_AMP-binding.1"
                     /domains="nrpspksdomains_FRZ02_RS31005_PCP.1"
                     /domains="nrpspksdomains_FRZ02_RS31005_Thioesterase.1"
                     /final_module
                     /locus_tags="FRZ02_RS31005"
                     /monomer_pairings="Gln -> Gln"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       3595..3633
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FRZ02_RS31005_0001"
                     /evalue="9.70E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="FRZ02_RS31005"
                     /protein_end="94"
                     /protein_start="81"
                     /score="12.7"
                     /tool="antismash"
                     /translation="WGILTAGAAYLPL"
     CDS_motif       3826..3885
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FRZ02_RS31005_0002"
                     /evalue="4.80E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="FRZ02_RS31005"
                     /protein_end="178"
                     /protein_start="158"
                     /score="31.3"
                     /tool="antismash"
                     /translation="AYVIHTSGSTGSPKGVMIEH"
     misc_feature    3904..3906
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       4411..4494
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FRZ02_RS31005_0003"
                     /evalue="4.40E-12"
                     /label="NRPS-A_a6"
                     /locus_tag="FRZ02_RS31005"
                     /protein_end="381"
                     /protein_start="353"
                     /score="38.2"
                     /tool="antismash"
                     /translation="GRSGELFIGGVQVARGYAGRPEATRERF"
     CDS_motif       4579..4638
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FRZ02_RS31005_0004"
                     /evalue="1.30E-08"
                     /label="NRPS-A_a8"
                     /locus_tag="FRZ02_RS31005"
                     /protein_end="429"
                     /protein_start="409"
                     /score="27.1"
                     /tool="antismash"
                     /translation="EGRTDNQVKLRGYRVELEEV"
     aSDomain        6154..6360
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FRZ02_RS31005_PCP.1"
                     /evalue="1.30E-19"
                     /label="FRZ02_RS31005_PCP.1"
                     /locus_tag="FRZ02_RS31005"
                     /protein_end="1003"
                     /protein_start="934"
                     /score="62.2"
                     /tool="antismash"
                     /translation="ERWLAGEWGRLLGYEDVSTRDEFFASGGNSLHSVALVNRINRRFG
                     TQFPLQTVFEAPTLAELAARVAER"
     aSDomain        6421..7149
                     /ASF="active site serine inconclusive"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FRZ02_RS31005_Thioesterase.1"
                     /evalue="7.60E-25"
                     /label="FRZ02_RS31005_Thioesterase.1"
                     /locus_tag="FRZ02_RS31005"
                     /protein_end="1266"
                     /protein_start="1023"
                     /score="80.6"
                     /tool="antismash"
                     /translation="FVWPGLGGYPMNLRPLALGFDGERSVTAVQAQGINTGETPHPTVR
                     RMAEADIAEIRRVQPSGPYTLWGYSFGARVAFEAAWQLEQAGEKVEDLLLICPGNPRVR
                     TEDGRRRGREASFANPVYVTILHSVFTGTTHGPAVAACLRETTDEDSFVAFVHRTTPAL
                     SPETIRRITQVVAATYAFEYTFSELRERRVEAPVTVFKAHGDDYSFLEDSSGYSARPPR
                     VVPLTGDHYEVLRERGVAELI"
     CDS_motif       6595..6669
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FRZ02_RS31005_0005"
                     /evalue="4.90E-11"
                     /label="NRPS-te1"
                     /locus_tag="FRZ02_RS31005"
                     /protein_end="1106"
                     /protein_start="1081"
                     /score="35.0"
                     /tool="antismash"
                     /translation="QPSGPYTLWGYSFGARVAFEAAWQL"
     gene            7235..7489
                     /locus_tag="FRZ02_RS31010"
                     /old_locus_tag="FRZ02_31010"
     CDS             7235..7489
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003946751.1"
                     /locus_tag="FRZ02_RS31010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_31010"
                     /product="tautomerase family protein"
                     /protein_id="WP_003946751.1"
                     /transl_table=11
                     /translation="MPHVSIQHFSRTFSEAEKAELVEALTETVTRVFGTGPEAVSIAVE
                     PVDPSRWTELVWQPQIEARPHLLWKQPGYTPNATTEGTR"
     gene            7486..8427
                     /locus_tag="FRZ02_RS31015"
                     /old_locus_tag="FRZ02_31015"
     CDS             7486..8427
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003946750.1"
                     /locus_tag="FRZ02_RS31015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_31015"
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="WP_003946750.1"
                     /transl_table=11
                     /translation="MNPAAPSIPVVHTEEVREALHEGRPVVALESNVITHGLAYPDNAQ
                     TAHQVEAAVRKGGAVPATICLDGGAIRVGMSEDDIERFASEPGIPKVSSRDLPVVLARG
                     GRGATTVASSLVAAELAGIPFFASAGLGGVHRGAQATMDVSSDLIQLTRSKVAVVCAGA
                     KMILDLKLTMEYLETQCVPVISTGSDDFPAFYCASSGVRSPHRVDDDALLARIVATHWA
                     AGHPGSVVITTPPRPEDAVDSAEAEQAITEALAQAERDGVSGQGLTKYLMRAVDKATGG
                     RTAQANMAVLISTAETGGRLAAAYARHLQSGN"
     CDS             complement(8492..>8670)
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003946749.1"
                     /locus_tag="FRZ02_RS31020"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_31020"
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /pseudo=""
                     /transl_table=11
                     /translation="PTPGFAATLAYYDSLRAPRLPAALTQGQRDYFGAHTYHRTDRTGT
                     FHTLWGGDRSETEA"
     gene            complement(8492..>8672)
                     /locus_tag="FRZ02_RS31020"
                     /old_locus_tag="FRZ02_31020"
                     /pseudo=""
ORIGIN
        1 gccctacccc gctccggcgc ctccggagcc ccagcccgac ccgcgctgag caccgggacg
       61 gcccggcacc cgtggaactc cacgggtgcc gggccgtccg cgttcgtctg cggcccggac
      121 gggtccgccc atgatcgttg tcggtccgcc ggaggtggcc ccggtgacgg cgttcgggcg
      181 gatcggcgcc gtccctcctc cgctccggcc gtccgcgcac cgcctcgtcc cggcctgtcg
      241 cgcccagttc tccagccgtc ggcgcataac gtccggctcc tattgggctc tgctttggcc
      301 ggtgttcatt tcccctggcc gggagtgctt ttcgcgttac cggaatgctg gaatccgcag
      361 ggcgcgcaca gcgatactca cctttcgttc atctgggacg aaatcgcctg cgcgcggccg
      421 gtatcgaaac cctggcggcg taaacctctg caggtagagg ccaaatgtgg cccgatttca
      481 gcagcgcggt cgaaggaacg ttgaatatct cggcctcctg gcgcactggc cggtccggca
      541 tggctgacga ttgacaggtt cttattgagc cacctagtgt tcccgaccgt tcggggtcgg
      601 cggtctggac cccgaaacgt ccgatgtgac ttgggggagg ctgcatcgag cccccagtct
      661 ggtattgcgg cgtacgtgtt cgccgttcca tcttccacgg ggggtcgtct tgatttctgc
      721 ccgaaatgtg ttgctgctgc cgcagaccaa ccagttacgc gcgctccaca ccgtcatacg
      781 cgacagaagc gcgtcgggaa gggacttcga ggtctactcg cggcgcatca tccggctgct
      841 gctggaggcg gggctcgatc tgcttccttt cgggaaacgc gacgtcacca cgccggtcgg
      901 cgagacctac cacggactcg aattcactcc gcaggtctgc ggggtttcgg tggtcagggc
      961 cggggaaagc atggaggctg agctgcgaga catgatcccc ggaatcccta taggaaagat
     1021 cctgatccag cgggacaagc ggacaaagca gcccgagctg ttctacaagc atcttcccgg
     1081 cgacatcggc tcgggccacg tgctgctcct ggaacccatg ctggccaccg ggggttcggc
     1141 cctcgccgcc ctggacgtgc tgcgcgacgc gggagttccg gaggagaacg tcgttctggt
     1201 caacttcctg gcgtccccca cgggactgga gcgtgtcagc ctcgccgccc cggagatgaa
     1261 gatcgtgacg tcctcggtgg aagacggaat gaacgagcac gcgttcatgg tgcccggcat
     1321 cggcgacttc ggggacaggt tcttcggcac ggttccgacg gctgcctcat gaggcagcgt
     1381 gccggattcg actccgcgct caccgtcctc gctccggtcg tctggggcag cacctatctg
     1441 gtgacgacgc agttccttcc gccggaccgg ccgctcctcg ccgccgccgt gcgggccctg
     1501 ccgggcgggc tgatcctcac gctggcgggg aggaagctcc cgcaaggcca ttggtggtgg
     1561 cggatcctgg cgctcggcgc cctcaacatc ggcttcttct tctatctgct cttcgtcgcc
     1621 gcctaccacc tgcccggcgg tgtcgccgcg ctggtcggct ccatccagcc cgtcttcgtc
     1681 ctcctgctgt ccgcgctgct gctgcgcgag aagatccgca gctggcaggt gggcgcctgt
     1741 gtcctcggta cggccggggt gggcatgctc gtcctggggc cggaggccgg actcgacgcg
     1801 gtcggggtgg ccgccggtct ggggggtgcc gcatgcatgg cggcgggcat cgtcctgacg
     1861 aagcgctggg gccggcctcc gggggtggga ctgctcacct tcaccggctg gcagctggcc
     1921 gtgggcggtc tgctcatcgc cccggtggcg ctggcggccg aagggcttcc cgaccggctg
     1981 acctggccga acttcgccgg gttcgcctac ctcagcctca tcggtgccct ccttgcctac
     2041 gccgtctggt tccgtggcat cgagcggctc ccggccctga cggtgtcgct gctcggcttc
     2101 gcgagcccgc tcgccgccac cgtcctcggc tacctcttcc tgcgacaggg cctgacgctc
     2161 gtccagggcg ccggagccgt cgccgtgatc agcgcggtcc tgctggccca gcgctccggc
     2221 gcgcgggagt cccgggcggc ccggaagtcc ggcccgccac cggaaccggc ggaggcgggc
     2281 acggagacgc ccgcggccgc cgggcccgcc catccggcac cgccttcgcc gcagcccggt
     2341 gaccggtcgg cggcacgttc ctcccagccg atcccggaga gccgaaagat cacggaaaag
     2401 gagcagtcat gacgcggccc gtcctgttcg atctcgacgg gaccctcgtc gacaccccga
     2461 acgccatcgt cgagaacttc accgccaccc tggccgagct gggcgctccg gccgcctccc
     2521 ccgaggagat ccggtccacc atcgggctcc cgctcgagaa ggcgttcgcc cagctcatcg
     2581 gagcctccgt gcccgccgcc accctcgacg cggccgtgcc gtgctaccaa cggctgtacc
     2641 gcgaacagat cgtgccgaag gccaagtcgt tgctgtaccc cggagtcgtc gacggcctgg
     2701 ccgagctgtc cgaggcgggc ctgctgctcg ccatcgccac cagcaagcac accgcgagtg
     2761 ccgaggtgct gctgacggcg gccggcatcc gggaccggtt cgccgtggtc gtcggcgcgg
     2821 acgccgtcac ggaaccgaaa ccggctccgg agacgggcct gctcatcgcc gccgaactgg
     2881 gccatccggc cgcccgggcc atcatggtcg gtgacaccac ccacgacatc tcgatggcgc
     2941 acaacgccgg tatgcgcagc gtcgcggtca cctacggcat ccactcgcct ggccaactgg
     3001 ccgcggtccg gcccacctgg acggtgcggg acttccccac cgccgtggag cggatcctcc
     3061 gcgccgactg accacgcccg accccggcgc gcccgtgccc agcgcgcggc cccgcccgtc
     3121 acccgtcact cccggcccgg gggcagctct tcccccgggc tgtccggcaa ccctgctccc
     3181 ggcggccggc cgtcgggcca tgccgtgatg ccgtctcgca ctcccgcagg gcacaacagg
     3241 gccgaccgcg cccatgagtt cacctcgcac gatcagcacc agcctgcacc ggcgcccgcc
     3301 gccgacgcgg ccgtacccat cgcagatcac acactcgggg gatcgtcaaa gatgagcacc
     3361 agcacgccgc cacccgccac ccgccagcac gcctccctgc ccgccctgct gcgccaccag
     3421 gcccgtgagc gcggcgacga caccgccgtg acagcggcgg agggccgtct caccttcgcg
     3481 gaactccact ccgcggccac gcggatgggc tcccacctgg tcggcgccgg agtgtcgccc
     3541 gacacctgcg tcgggctctt cgtggagccc tccgccgacc tggtgacagg tgtgtggggc
     3601 atcctcaccg caggcgcggc ctatcttccg ctctcgcccg actacccgga ctcccggctt
     3661 cgccacatgg cggcggacgc cggagtcggc gtggtcgtca cccagagcca cctgcgcgcc
     3721 aggctgcacg gtttggttcc cgcgggcacc ctggtcgtca ccgtggacga ggccacggac
     3781 gccgacggcg cgcaccccct tcccgagccc cgggaggacc acctcgccta cgtcatccac
     3841 acctccggca gcacgggcag ccccaagggc gtgatgatcg aacaccgcag tgtcgtcgcc
     3901 cagttacggt ggctgacgcg ccgtgggtat ctcggcccgg cggtctccgt cctgcagaag
     3961 acgccgatca gcttcgacgc ggcgcagtgg gagatcctgg cctgtgcggc gggggcgcgg
     4021 gtggtcatga gtccgccggg gtccttccgc gacccgcagg cggtcatcga cgcgatccac
     4081 acgtacggcg tcacctgcct gcagggggtg cccaccctgc tgcgggccct cgtggacacc
     4141 ggtgagctgg agacctgctc gacactgacc cggatcttct ccggcggcga agccctcacc
     4201 cgggccctcg cgcaggagct gctccgcacc ctgccaggcg tctcgctggt caacctctac
     4261 ggcccgaccg agacgaccat caacgcgacg tccctcgagg tctccccgga caccctcgac
     4321 gacgaggacc tccacatcct gcccatcggc agacccgcgg acggcgtcac ctgccacatc
     4381 ctggacgaga acctccggcc cgtcggcacc gggcggagcg gggaactgtt catcggcggg
     4441 gtccaggtgg cccgcgggta cgccggccgg cccgaggcga cccgggagcg cttcctcacc
     4501 gcgccgtccg cccctggcga gcggctgtac cgcacgggcg acctcgcccg gtggcggccc
     4561 gacggcacga tccagtacga gggccgcacc gacaaccagg tcaaactccg cggctaccgg
     4621 gtcgagctgg aggaggtcgc gtcgcgcgtc gaggagcaca tgtgggtgcg caaggccgcc
     4681 gcggtggtca cggacaaccc ccgcacgggc tcgcaggtcc tggtcgccgc cgtcgaactc
     4741 aacgaacaca cggcggcggt gatggaccag gggcgctccg acgccgggca ccaccagtcc
     4801 aagtcctcca agctccaggt gaaggcccag ctcgccaacc ccgggctgcg ggaaccggaa
     4861 gaactggcag gccggaccgt cgtcccgctg gccggcgccg aggcgatgcc ggagcagcga
     4921 agagccgcct tcgcccgcaa gtcgtaccgg tactacgagg ggggacgggc gaccgccgag
     4981 cagctcaccg cgctgctcac ctcgcccccg ccgcccgctg ccgccgcccg ccggccgggc
     5041 ggactcaccc cgcaggagct cggctccctc ctgcggtggc tcggtccgca ccgcagccct
     5101 gaccgactgc tgccgaagta cgcctacgcg tcccccggag ccctgtacgc cacccagctg
     5161 tacctggaga tcgacggtgt ggccgggctg tcgggcgtct actaccacca ccccttcgac
     5221 cacaccctcg tacgcgtcgg agaccacccg gcgccgggcc gcaccgccga gctgaagatc
     5281 cacttcctgg ggaagcggcg ggccgtcgag cccgtctaca agaacaacat cgtcgaggtg
     5341 ctggagttcg aggccgggca catgctcggg gtcctggagg aggtgctgcc gggcatgggc
     5401 ctcatggtgg ccccgcacgg cttcgacccg gacgccgcgg cccggctcga cgtcgcggcg
     5461 gacgaccact acctgggcac cttcgcggtg gtcccgcacg acggccggcc ccgccccgag
     5521 gaggtggagc tgttcctcca ggtgcacggt gaccgggtcg agggactgcc cggcggcctc
     5581 taccggcacc aggacggcac gctggtccgt ctcggggacg aggtggtgga gcagcgccac
     5641 gtcatcgcga tcaaccaggg cgtgtacgag cgggcctcgt tcggcatcgg cgccgtcgcg
     5701 cggacgagcg acgactggcg ccggtacatg gtgctcggga ccctgctgca ccgcctccag
     5761 cgcgtcgggg gcatggggct gatgtcggcg gggtactcct cgcggaccgg ccacccgctg
     5821 cccgccgcgc agcgcctgga cgacctcctg agccgcgcct cggtgcgcgc tgcccccgcc
     5881 tcgtacttct tcgtgggggg tccggtctcc gcggaacagg ccgcgcacga gggcatgaac
     5941 gaggacgccg tgcacaccaa ggggccggcg gagatgatcc gtgacgacgc cgcccagttc
     6001 ctccccgact acatggtccc ggcgcgggtc gtcgtggtgg accagctgcc cgtcaccccc
     6061 aacggcaaga ccgatctcaa agccaccgcc ctcctgcccg aggtggccgc cggccggaac
     6121 accaccccgc acgtcgcacc ggcgacgccc accgaacggt ggctggccgg cgagtggggc
     6181 cgcctgctcg gctacgaaga cgtctccacc agggacgagt tcttcgcctc cggaggcaac
     6241 tccctgcact ccgtggccct ggtgaaccgg atcaacagac ggttcgggac acagttcccg
     6301 ctccagacgg tgttcgaggc acccaccctc gccgaactgg ccgcccgcgt ggcggagcgc
     6361 gccccggcac ccgcctccag gctgatacgc ctcaacagcg acggcgcccc ggcccgggcc
     6421 ttcgtctggc ccggcctggg cggctacccc atgaacctgc gcccgctcgc cctgggcttc
     6481 gacggtgagc gctcggtgac cgccgtacag gcccagggca tcaacaccgg cgagaccccc
     6541 caccccaccg tccggcggat ggcggaggcg gacatcgccg aaatacggcg cgtgcagccc
     6601 tcggggccgt acaccctctg gggctactcc ttcggcgcgc gggtggcctt cgaagccgcc
     6661 tggcagctgg agcaggcggg cgagaaggtg gaggacctgc tgctgatctg cccgggcaac
     6721 ccgcgcgtcc ggaccgagga cggccggcgc cggggccgcg aggcatcgtt cgccaacccg
     6781 gtctacgtga ccattctcca ctccgtgttc accggcacca cccacgggcc cgccgtggcc
     6841 gcctgcctgc gcgagaccac cgacgaggac tccttcgtcg ccttcgtcca tcgcaccacg
     6901 cccgcgctgt ccccggagac gatccggcgc atcacccagg tcgtcgcagc gacctacgcg
     6961 ttcgaataca ccttcagcga actgcgggag cgtcgggtcg aggcaccggt gaccgtcttc
     7021 aaggcgcacg gggacgacta ctcgttcctg gaggacagtt ccggctactc ggccaggcca
     7081 ccgcgggtgg tgcccctcac cggcgaccac tacgaggtgc tgagggagcg gggcgtggcc
     7141 gaactgatct ccggcatacg cgccttggac ctcttcgcct agaccctgac ccggcctcgg
     7201 ccacgcagaa tcgcacccgg gaggaacccg ccacatgcca cacgtcagca tccagcactt
     7261 cagccgtacg ttctccgagg ccgagaaggc cgagctggtc gaagccctga ccgagacggt
     7321 cacccgggtc ttcggcaccg ggccggaggc ggtgtccatc gccgtggagc cggtcgaccc
     7381 gtcccggtgg accgagctcg tctggcagcc gcagatcgag gcccgcccgc acctgctgtg
     7441 gaagcagccc ggatacaccc cgaacgccac caccgaagga acccgatgaa tcccgcagca
     7501 ccgtccattc cggtcgtcca caccgaagaa gtccgcgagg ccctccacga gggccgcccg
     7561 gtcgtcgcgc tggagtccaa cgtcatcacc cacggactgg cctacccgga caacgcacag
     7621 accgcccacc aggtggaggc ggccgtccgc aagggcgggg ccgttcccgc cacgatctgt
     7681 ctggacgggg gcgcgatcag ggtcggcatg agcgaggacg acatcgaacg gttcgcctcc
     7741 gagccgggca tcccgaaggt ctccagccgc gacctgccgg tggtcctcgc caggggcggg
     7801 cgcggtgcca cgaccgtcgc ctcctccctg gtcgccgccg aactcgccgg catcccgttc
     7861 ttcgcctcgg ccgggctcgg cggggtccac cggggcgcgc aggccacgat ggacgtctcc
     7921 tcggacctga tccagctgac ccgttccaag gtggcggtcg tctgcgcggg cgccaagatg
     7981 atcctcgacc tgaagctgac catggagtac ctggagaccc agtgcgtccc ggtgatctcc
     8041 accggctcgg acgacttccc ggccttctac tgcgcctcca gcggcgtgcg ttccccacac
     8101 cgcgtggacg acgacgctct cctcgcccgc atcgtcgcca cccactgggc cgccggccac
     8161 cccggctccg tcgtcatcac cacacccccg cgccccgagg acgcggtgga ctccgccgag
     8221 gccgagcagg cgatcaccga ggcactggca caggcggagc gggacggggt gagcggccag
     8281 ggtctcacca agtacctgat gcgggccgtc gacaaggcga ccggaggccg gacggcccag
     8341 gccaacatgg ccgtgctgat ctccaccgcc gagaccgggg gccggctcgc cgccgcctac
     8401 gcccggcacc tgcagtcggg gaactgaccg agggcgcacg ccggccggcc cggggatcct
     8461 gccccgggcc ggccggcgtg cggcgacggt ctcaggcctc ggtctcgctc cggtcgccgc
     8521 cccagagggt gtggaaggtg ccggtgcggt cggtgcggtg gtaggtgtgg gcgccgaagt
     8581 agtcgcgctg gccctgggtg agggcggcgg gcaggcgcgg ggcgcgcagg gagtcgtagt
     8641 aggcgagggt ggccgcgaag ccgggggtgg gg
//
