LOCUS       NZ_VOGX01000028        10399 bp    DNA     linear   CON 15-OCT-2024
DEFINITION  Streptomyces albidoflavus strain 77
            scaffold11_cov50_3246940_3564226, whole genome shotgun sequence.
ACCESSION   NZ_VOGX01000028
VERSION     NZ_VOGX01000028.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces albidoflavus
  ORGANISM  Streptomyces albidoflavus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces albidoflavus group.
REFERENCE   1  (bases 1 to 317287)
  AUTHORS   Pylro,V., Dias,A., Andreote,F., Varani,A., Andreote,C., Bernardo,E.
            and Martins,T.
  TITLE     Draft Genomic Sequences of Streptomyces misionensis and Streptomyces
            albidoflavus, bacteria applied for phytopathogen biocontrol
  JOURNAL   Microbiol Resour Announc (2019) In press
REFERENCE   2  (bases 1 to 317287)
  AUTHORS   Pylro,V., Dias,A., Andreote,F., Varani,A., Andreote,C., Bernardo,E.
            and Martins,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2019) Biology, UFLA, Universidade Federal de
            Lavras, Lavras, MG 37200000, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-JUL-2019
            Assembly Method                   :: SPAdes v. 3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 36.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007896905.1-RS_2024_10_13
            Annotation Date                   :: 10/13/2024 04:20:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,551
            CDSs (total)                      :: 6,456
            Genes (coding)                    :: 6,343
            CDSs (with protein)               :: 6,343
            Genes (RNA)                       :: 95
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 5 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 34 of 113
            Pseudo Genes (incomplete)         :: 81 of 113
            Pseudo Genes (internal stop)      :: 8 of 113
            Pseudo Genes (multiple problems)  :: 8 of 113
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:41:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 93339
            Orig. end                         :: 103738
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VOGX01000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(106..121)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.95"
                     /tool="antismash"
     gene            complement(293..1384)
                     /locus_tag="FRZ02_RS16720"
                     /old_locus_tag="FRZ02_16720"
     CDS             complement(293..1384)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019982886.1"
                     /locus_tag="FRZ02_RS16720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16720"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_146581750.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 2.9e-17, bitscore:
                     57.0, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEDMVLHVKGRILVGPEEARDELWVVGGRITFTRPPGAREVRTV
                     EGWALPGLVDAHCHVGLDRHGPVAAEVAEKQALTDRDAGALLLRDAGSPSDTRWMDDRD
                     DLPRVIRAGRHIARTRRYIRNYAHEIEPDQLVAHVSQEARRGDGWVKLVGDWIDRGAGD
                     LTPCWPREAVEAAIAEAHRLGARVTAHCFAEDSLRDLVEAGIDCIEHATGLTEETIPLF
                     AERGVAIVPTLVNIATFPDLAAGGQAKFPDWSAHMRRLYERRYDTVRAAWDAGIPVFVG
                     TDAGGSLPHGLVAAEVAELVTAGIPPLDALSATAWGARDWLGRPGLTEGAPGDLVVYAS
                     DPRADVRVLAAPRRVVVGGKVVA"
     gene            1459..2574
                     /locus_tag="FRZ02_RS16725"
                     /old_locus_tag="FRZ02_16725"
     CDS             1459..2574
                     /NRPS_PKS="Domain: Aminotran_5 (73-284). E-value: 1.3e-30.
                     Score: 98.5. Matches aSDomain:
                     nrpspksdomains_FRZ02_RS16725_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947419.1"
                     /locus_tag="FRZ02_RS16725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16725"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_030767898.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.2e-24, bitscore:
                     81.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLTPLTAAHFANIERRVAALLSTAQDVVITQGEALLPLE
                     GCIRGVAGPDTTALNVITGPYGQTFGDWLRDCGARVVDLAVPYDAAVGADQVAEALAEH
                     PEISFVSLVHAEAATGNTNPVAEIGRVVREHGALFMVDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWQRMAANPRAPRRSYLSLLDWKERWIDAGRTALLHAP
                     AQLEMVALQACVERIEDEGLETVIGRHTRAAAATRAGVKALGGGFAPYVREARHAAPVA
                     TTLRAPEGVDASELVARALAADPVLPLVAGGGALAKEMIRVNHYGTAAAGSTVHSCLSA
                     LGAALTDLTGRSVDPDAARRAATAAWERPAG"
     aSDomain        1678..2310
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FRZ02_RS16725_Aminotran_5.1"
                     /evalue="1.30E-30"
                     /label="FRZ02_RS16725_Aminotran_5.1"
                     /locus_tag="FRZ02_RS16725"
                     /protein_end="284"
                     /protein_start="73"
                     /score="98.5"
                     /tool="antismash"
                     /translation="LRDCGARVVDLAVPYDAAVGADQVAEALAEHPEISFVSLVHAEAA
                     TGNTNPVAEIGRVVREHGALFMVDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVS
                     AVSVSERAWQRMAANPRAPRRSYLSLLDWKERWIDAGRTALLHAPAQLEMVALQACVER
                     IEDEGLETVIGRHTRAAAATRAGVKALGGGFAPYVREARHAAPVATTL"
     misc_feature    2697..2712
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.17"
                     /tool="antismash"
     misc_feature    complement(2712..2728)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    2715..2728
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.78"
                     /tool="antismash"
     misc_feature    2835..2850
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     misc_feature    complement(2846..2861)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    2856..2871
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    2895..2913
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 21.64"
                     /tool="antismash"
     misc_feature    2968..2978
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.79"
                     /tool="antismash"
     misc_feature    2990..3002
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.12"
                     /tool="antismash"
     misc_feature    3016..3028
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3069..3575
                     /gene="ectA"
                     /locus_tag="FRZ02_RS16730"
                     /old_locus_tag="FRZ02_16730"
     CDS             3069..3575
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412174.1"
                     /locus_tag="FRZ02_RS16730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16730"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_008412174.1"
                     /transl_table=11
                     /translation="MKEMPEDLRLDHPGVEDGAAIWRIARDSRTLDLNSSYSYLLWCRD
                     FAGTSVVARDAAGEPVGFVTGYFRPERPHTLVVWQVAVDASQRGRGLAAALLDGLTDLL
                     REKGRLDGIETTITPGNTPSERLFTSYAKRHGAELEREVLFGAGLFPDGGHDPEVLYRI
                     GPFTD"
     gene            3695..4966
                     /gene="ectB"
                     /locus_tag="FRZ02_RS16735"
                     /old_locus_tag="FRZ02_16735"
     CDS             3695..4966
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 1.8e-75.
                     Score: 246.0. Matches aSDomain:
                     nrpspksdomains_FRZ02_RS16735_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="FRZ02_RS16735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16735"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_018470508.1"
                     /sec_met_domain="Aminotran_3 (E-value: 4e-81, bitscore:
                     266.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRSWPAVFDRAQGSYLYDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALIDYIERDGITHGLDMGTTAKRAFLETFQNVLLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVQDFLWFERLLEDQGSGLNTPAAVIVETIQGEGGINVARP
                     EWLRALADLCKRRDMLLIVDDIQMGCGRTGPFFSFEEAGITPDIVTLSKSIGGYGMPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDAYWSDGQMEKQTLARGEQVEQALT
                     AILDEHGGEAIVSVRGRGLVWGMEFADKSRAGAVSKRAFELGLLVETSGPESEVVKLLP
                     PLTVSPDELDEGLRILARAVRETA"
     aSDomain        3779..4792
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FRZ02_RS16735_Aminotran_3.1"
                     /evalue="1.80E-75"
                     /label="FRZ02_RS16735_Aminotran_3.1"
                     /locus_tag="FRZ02_RS16735"
                     /protein_end="366"
                     /protein_start="28"
                     /score="246.0"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGHTYLDFFAGAGALNYGHNNPVLKRALIDYIE
                     RDGITHGLDMGTTAKRAFLETFQNVLLRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVQDFLWFER
                     LLEDQGSGLNTPAAVIVETIQGEGGINVARPEWLRALADLCKRRDMLLIVDDIQMGCGR
                     TGPFFSFEEAGITPDIVTLSKSIGGYGMPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDAYWSDGQMEKQTLARGEQVEQALTAILDEHGGEAIVSVRGRGLVWGMEFA"
     gene            5001..5399
                     /locus_tag="FRZ02_RS16740"
                     /old_locus_tag="FRZ02_16740"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947422.1"
                     /locus_tag="FRZ02_RS16740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16740"
                     /product="ectoine synthase"
                     /protein_id="WP_003947422.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.9e-56, bitscore:
                     182.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIENTDRHVKSASGTWESKRIVLAKEKVGFSLHETILY
                     AGTETDMWYANHIEAVLCVEGEAELTNRETGETHWIEPGTMYLLNGHEKHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEED"
     gene            5403..6299
                     /gene="thpD"
                     /locus_tag="FRZ02_RS16745"
                     /old_locus_tag="FRZ02_16745"
     CDS             5403..6299
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010637026.1"
                     /locus_tag="FRZ02_RS16745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16745"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_010637026.1"
                     /sec_met_domain="PhyH (E-value: 1.8e-38, bitscore: 127.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVASERTDLYPSRTATEVTIPRKDPVVWSPQDAPGPITRSDLQS
                     YEDNGFLAVEELITPEEVGVYRSELDRLVADPLIRADERAIVEPKSEKVRSVFEIHKIS
                     EIFAGLVRDERLLGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPR
                     MRAVSVSIALTENYDTNGGLMIMPGSHRTYVGCNGVTPKDNYKRSLQMQDAGIPSDEVL
                     TEFADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVDNAAEEPFAA
                     PVRRPEFIGARDFTPVR"
     gene            complement(6473..7534)
                     /locus_tag="FRZ02_RS16750"
                     /old_locus_tag="FRZ02_16750"
     CDS             complement(6473..7534)
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947424.1"
                     /locus_tag="FRZ02_RS16750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16750"
                     /product="alkene reductase"
                     /protein_id="WP_010637028.1"
                     /transl_table=11
                     /translation="MSTAFEPLDLSGTKLPHRIAMAPMTRSRSGAAGVPTALMAEYYAQ
                     RASAALIVTEGIQPSAVGQGYPDTPGLHSAEQVTGWRAVTSAVHAAGGRIFAQLMHAGR
                     IGHPSLLPEGEVNVAPSAVAATGQVYTAEGPQDFVTPRELTDEEIRATLADYVTAARNA
                     VDAGFDGVELHGANGYLIHQFLAPGSNRRTDGWGGSVEGRIRFAAEVTEAVVAEIGAAR
                     TALRLSPGNAFNSIEEPEPEPEYTALVARIAPLGLAYLHLAEDGPARRALTLRLRELFD
                     GPLMLNPADSPTGPEALALVEEGTADLVSYGQLFLANPDLPARLRAGGPFNEARRATMY
                     GGGAEGYTDYPAL"
     gene            7706..8218
                     /locus_tag="FRZ02_RS16755"
                     /old_locus_tag="FRZ02_16755"
     CDS             7706..8218
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470511.1"
                     /locus_tag="FRZ02_RS16755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16755"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_146581752.1"
                     /transl_table=11
                     /translation="MNEATGPEQTPCTWPAERGAAPPGGPVGHLVTRVARLHRTAAGRA
                     LRESGLYPGQEIVLGHLWEADGPVRQSDLIKAVELDPSTVTKTLQRLEQSGHVTRAPDP
                     ADRRAVLVEATELSCALSRQVESAWAGLEEHALAGLTPADRDELLRLLTAVEANLCDRA
                     GGCPEAE"
     gene            complement(8229..9278)
                     /locus_tag="FRZ02_RS16760"
                     /old_locus_tag="FRZ02_16760"
     CDS             complement(8229..9278)
                     /NRPS_PKS="Domain: Aminotran_5 (55-343). E-value: 1.2e-22.
                     Score: 72.3. Matches aSDomain:
                     nrpspksdomains_FRZ02_RS16760_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018895195.1"
                     /locus_tag="FRZ02_RS16760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16760"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_018895195.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.1e-18, bitscore:
                     61.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLARSLAEDEFKPSRTHLATAGTGLLPLSAARAVHEAVDAAAAG
                     TPPDMFGARDAARASFARLVGVPATRVATGSSVATFTGLIAAALPDGAEVLTAEGDFSS
                     LVNPFHVRPGVSVRTVPLERIAEHVTGTTALVAVSAVQSADGRRADLAAVREAARAHGA
                     RTLVDASQAAGWDTFEAGAYDYTVAVGFKWLLAPKGTAFLTVPPEPGALPPLLAGWVAG
                     DSPFDSCYGPVTGLAPDARRYDESPAPLVWTGAQHALALLERIGTEAVGAHDVALADRF
                     RERLTRAGHAPVPAPGSAIVSVPGLGERAAALGERGITVSDRAGMLRAAFHLYNTESDV
                     DRLVAALDG"
     aSDomain        complement(8250..9113)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FRZ02_RS16760_Aminotran_5.1"
                     /evalue="1.20E-22"
                     /label="FRZ02_RS16760_Aminotran_5.1"
                     /locus_tag="FRZ02_RS16760"
                     /protein_end="343"
                     /protein_start="55"
                     /score="72.3"
                     /tool="antismash"
                     /translation="AARASFARLVGVPATRVATGSSVATFTGLIAAALPDGAEVLTAEG
                     DFSSLVNPFHVRPGVSVRTVPLERIAEHVTGTTALVAVSAVQSADGRRADLAAVREAAR
                     AHGARTLVDASQAAGWDTFEAGAYDYTVAVGFKWLLAPKGTAFLTVPPEPGALPPLLAG
                     WVAGDSPFDSCYGPVTGLAPDARRYDESPAPLVWTGAQHALALLERIGTEAVGAHDVAL
                     ADRFRERLTRAGHAPVPAPGSAIVSVPGLGERAAALGERGITVSDRAGMLRAAFHLYNT
                     ESDVDRL"
     gene            complement(9404..10129)
                     /locus_tag="FRZ02_RS16765"
                     /old_locus_tag="FRZ02_16765"
     CDS             complement(9404..10129)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947427.1"
                     /locus_tag="FRZ02_RS16765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_16765"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_146581754.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFDKALAAFPHRDRIEVVHRSFELDP
                     ARPKGDPELVLPMLAKKYGMSEAQAAEAERNLGQNAAAEGLPYLTEGRDHGSTFDMHRL
                     LHLAKARGRQDELLAVLYRANFAETATVFGDDERLVALAVEAGLEETEAREVLADAQRY
                     AAEVRADERAATELGASGVPFFVLDRRYGVSGAQPADVFTQALTQAWESHVPASGLAIP
                     AGAEGGEACGPDGCEVPKN"
ORIGIN
        1 cgcacctggg gacaggcggc cgccgacgac gtcgttgccg gaatctttac gcactccggg
       61 tgaaggggcg acaacctggg gtgacttcac tccaaagggt ggttttggtg cagacgttcg
      121 acccggcctg cacggccgtt cccccacgtc acccctgacc gccgtttccg ggcccccgaa
      181 gggcgcagct gggcgtacgg acggcgtacg cgggcgcgga cgtgcggcgg gggccggagc
      241 acaacgacgc tgctccggcc cccgtaacgg cccgtcaaca cgcttctcgc ggtcaggcga
      301 cgaccttgcc gccgaccacg acccggcggg gcgccgccag cacccggacg tcggcgcgcg
      361 ggtcgctcgc gtagaccacc aggtcgcccg gggcaccctc ggtcagtccg gggcggccga
      421 gccagtcgcg ggcgccccac gccgtggcgg agagggcgtc gagcggcggg atgccggcgg
      481 tgaccagttc ggcgacctcg gcggcgacca ggccgtgcgg gaggctgccg cccgcgtcgg
      541 tgccgacgaa gaccgggatg cccgcgtccc aggcggcgcg gaccgtgtcg taccggcgct
      601 cgtacagccg ccgcatgtgg gccgaccagt cggggaactt cgcctggccg ccggccgcga
      661 ggtcggggaa ggtggcgatg ttgaccaggg tggggacgat ggcgacgccg cgttcggcga
      721 agagcgggat ggtctcctcg gtgagcccgg tggcgtgctc gatgcagtcg atgcccgctt
      781 cgaccaggtc gcgcagcgag tcctcggcga agcagtgggc ggtgacccgg gcgccgagcc
      841 ggtgcgcctc ggcgatggcg gcctcgaccg cctcgcgcgg ccagcacggc gtcaggtcgc
      901 ccgcgccccg gtcgatccag tcgccgacca gcttgaccca gccgtccccg cgccgcgcct
      961 cctggctgac gtgggcgacg agttggtccg gctcgatctc gtgggcgtag ttgcggatgt
     1021 agcggcgggt gcgcgcgatg tggcggccgg cccggatgac gcggggcagg tcgtcgcggt
     1081 cgtccatcca gcgggtgtcg ctgggcgaac cggcgtcgcg cagcagcagc gccccggcgt
     1141 cgcggtcggt gagcgcctgc ttctcggcga cctcggcggc gaccgggccg tgccggtcca
     1201 ggccgacatg gcagtgggcg tcgaccaggc ccggcagcgc ccatccctcg acggtccgca
     1261 cctcgcgggc gcccggcggc cgggtgaagg tgatccgccc gcccaccacc cagagttcgt
     1321 ccctggcctc ctcgggaccg accagtatcc ggcccttcac gtgcagcacc atgtcttcgc
     1381 tcatgcacgg cagcgtagga cccggtgggt acgctcggac cggcagcccg acgatcgacc
     1441 ggcgaagaga gcaccaccgt gacgcacccc ctcctggacc tgaccccgct gaccgccgcg
     1501 cacttcgcga acatcgagcg ccgcgtcgcg gcgctgctct ccaccgcgca ggacgtcgtc
     1561 atcacccagg gtgaggcgct gctgccgctg gagggctgca tccgcggcgt ggcgggcccg
     1621 gacaccaccg ccctcaacgt gatcaccggc ccgtacgggc agaccttcgg cgactggctg
     1681 cgcgactgcg gggcgcgcgt ggtcgacctg gcggtgccct acgacgcggc ggtcggcgcc
     1741 gaccaggtcg ccgaggcgct ggcggagcac ccggagatca gcttcgtctc gctggtccac
     1801 gccgaggcgg cgaccggcaa caccaacccg gtggccgaga tcggccgggt ggtgcgcgag
     1861 cacggcgcgc tcttcatggt ggacgcggtg gcctcggtcg gcgccgagcc gctgctgccc
     1921 gacgcctggg gcgtggacct ctgcgtgatc ggcgcccaga aggcgatggg cggcccggcc
     1981 ggggtctcgg cggtctcggt gagcgagcgg gcctggcagc ggatggcggc caacccgcgg
     2041 gcgccgcgcc gctcctacct ctcgctgctg gactggaagg agcggtggat cgacgccggg
     2101 cgcaccgccc tgctgcacgc ccccgcccag ctggagatgg tggcgctcca ggcgtgtgtg
     2161 gagcggatcg aggacgaggg cctggagacg gtgatcggcc ggcacacccg ggcggcggcg
     2221 gccacccggg ccggcgtgaa ggcgctcggc ggcggattcg cgccgtacgt ccgcgaggcg
     2281 cgccacgcgg cgccggtggc gaccacgctg cgggcaccgg agggcgtgga cgcctcggag
     2341 ctggtggcgc gggcgctggc ggcggacccg gtgctgccgc tggtggccgg cggcggggcg
     2401 ctggccaagg agatgatccg ggtcaaccac tacggcacgg ccgcggccgg ctccacggtc
     2461 cactcctgcc tctccgcgct gggggcggcg ctgaccgacc tcacgggccg gtccgtcgac
     2521 ccggacgcgg cgcggcgcgc ggcgaccgcg gcctgggagc gccccgcggg ctgatccggc
     2581 cccgccagcc ccggcgggcc gtccggtgcg cggcgcacgc cgtgcgcggg gcggcccgtc
     2641 gcgcatttcc gttcccgggc acagcgaagt ccgcaccgac ttcggcgcgg acagcgaacg
     2701 atattcttaa tcggtagacc tattacaggg aattccgaat tctttcgcac tcaccttccc
     2761 ctattcgtct gcccgctctc cccgaagcta aacgcatcga ttttccggac accgaaaccg
     2821 cgtaattcgg acgcgtcacg cgtgcgttac gcacttgtga ctgactccac acggtgcccg
     2881 tttcgcggct tatatcctgg acaaatcacc atcttcaggc ggtcgttcgc gcaggcgcac
     2941 ccccgcgccc gataacatag aaagcgctgc catgtaaccc cgacccgcga tgcatttttc
     3001 agagtggatc ggtaaattca atttgcatga ccgccgcaca agccgacctt gaacgtggcc
     3061 gatccaccat gaaggaaatg ccagaggatt tgcgcctgga ccacccgggc gtcgaggacg
     3121 gggcagccat ctggcgtatc gcccgcgact cgcgcaccct cgacctcaac tcctcctaca
     3181 gctacctcct gtggtgtcgc gacttcgccg gcacctccgt ggtggcacgc gacgccgccg
     3241 gcgagccggt cgggttcgtg accggatact tccggcccga gcgcccgcac accctcgtcg
     3301 tctggcaggt cgccgtggac gcctcgcagc gcgggcgggg cctcgccgcc gccctgctgg
     3361 acgggctcac cgacctgctc cgtgagaagg ggcggctgga cgggatcgag accaccatca
     3421 ccccgggcaa cacgccctcc gagcgcctct tcacctccta cgccaagcgc cacggcgccg
     3481 agctggagcg cgaggtcctc ttcggtgccg ggctcttccc cgacgggggg cacgaccccg
     3541 aggtgctgta ccgcatcgga ccgttcaccg actgaccagc gcgaacgccc gcacagcaac
     3601 tccaggtccg gccggaaggc aagcccccgg ccgcccttcc gcacgtatcc ctgacttcgc
     3661 cccgcttcac cccccatctc ccaggagagc agtcgtgacc atcaccccgc ccgccctgag
     3721 cgtcttcgag acccttgagt cggaggtacg gagctactgc cgcagctggc ccgccgtctt
     3781 cgaccgggcc cagggcagct acctgtacga cgaggacggc cacacctacc tcgacttctt
     3841 cgccggcgcc ggcgccctca actacggcca caacaacccg gtgctgaaac gcgcgctgat
     3901 cgactacatc gagcgcgacg gcatcaccca cggcctggac atgggcacca ccgccaagcg
     3961 cgcgttcctg gagaccttcc agaacgtgct gctgcgcccg cgtgacctgc cgtacaaggt
     4021 catgttcccc ggcccgacgg gcaccaacgc cgtcgagtcg gcgctgaagc tggcccgcaa
     4081 ggtcaagggc cgcgagtcca tcgtctcctt caccaacgcc ttccacggca tgtcgctggg
     4141 ctcgctcgcc gtgaccggca acgccttcaa gcgcgccggg gccggcatcc cgctggtgca
     4201 cggcacgccg atgccgttcg acaactactt cgacggcacc gtgcaggact tcctctggtt
     4261 cgagcggctg ctggaggacc agggctcggg cctcaacacc cccgccgcgg tcatcgtcga
     4321 gaccatccag ggcgagggcg gcatcaacgt ggcccgcccc gagtggctgc gcgccctcgc
     4381 cgacctgtgc aagcgccgcg acatgctgct gatcgtcgac gacatccaga tgggctgcgg
     4441 ccgcaccggc ccgttcttct ccttcgagga ggcgggcatc accccggaca tcgtcaccct
     4501 gtcgaagtcc atcggcggct acggcatgcc catgtcgctc tgcctgttca agccggagct
     4561 ggacatctgg gagccgggcg agcacaacgg caccttccgg ggcaacaacc cggccttcgt
     4621 caccgccgcc gccgcgctgg acgcgtactg gagcgacggc cagatggaaa agcagaccct
     4681 cgcccggggt gagcaggtcg agcaggcgct caccgccatc ctcgacgagc acggcggcga
     4741 ggccatcgtc tccgtccgcg gccggggcct ggtctggggc atggagttcg ccgacaagtc
     4801 ccgcgcgggc gccgtctcca agcgcgcctt cgagctgggt ctgctggtcg agacttccgg
     4861 gccggagagc gaagtggtca agctgctgcc gccgctcacc gtgagcccgg acgagctgga
     4921 cgagggtctg cgcatcctcg cccgcgccgt ccgagagacg gcctgacaca caacgacgga
     4981 agaaagggca cgaccacact gtgatcgtcc gatcgttcaa ggacatcgag aacaccgacc
     5041 ggcacgtcaa gtcggcgtcc ggcacctggg agagcaagcg catcgtgctc gccaaggaga
     5101 aggtcggttt ctcgctgcac gagaccatcc tctacgcggg caccgagacc gacatgtggt
     5161 acgccaacca catcgaggcc gtgctctgcg tcgagggtga ggccgagctg accaaccgcg
     5221 agacgggcga gacccactgg atcgagcccg gcaccatgta cttgctgaac ggccacgaaa
     5281 agcacaccct gaggccgaag acagattttc gctgcgtctg cgtcttcaac cctcctgtga
     5341 ccggacggga ggaccacgac gagaacggcg tctacccgct gctgaccgag gaggactgag
     5401 tcatgactgt cgcgtcggaa cgcaccgatc tctacccgag ccggactgca accgaggtga
     5461 cgatcccgcg caaggacccc gtggtgtggt ccccgcagga cgcccctgga cccattacgc
     5521 ggtccgacct gcagtcgtac gaggacaacg gcttcctcgc cgtcgaggag ctgatcaccc
     5581 ccgaggaggt cggcgtctac cggtcggaac tggaccggct ggtcgccgac ccgctcatcc
     5641 gggccgacga gcgggccatc gtggagccca agtcggagaa ggtccgctcc gtcttcgaga
     5701 tccacaagat cagcgagatc ttcgccggcc tggtccgtga cgagcggctg ctgggccgcg
     5761 cccggcagat cctcggctcg gacgtctacg tccaccagtc ccggatcaac gtgaagcccg
     5821 gcttcggcgc ctccggcttc tactggcact cggacttcga gacctggcac gccgaggacg
     5881 gtctgccgag gatgcgcgcg gtgtccgtct cgatcgcgct gaccgagaac tacgacacca
     5941 acggcggcct catgatcatg ccggggtcgc accgcacgta cgtggggtgc aacggcgtga
     6001 cgccgaagga caactacaag cggtccctcc agatgcagga cgcgggcatc ccctccgacg
     6061 aggtgctgac cgagttcgcc gaccgccacg gcatcaagct cttcacgggc aaggccgggt
     6121 cggccacctg gttcgactgc aacgccatgc acggctccgg ggacaacatc accccgtacc
     6181 cgcgcagcaa cgtgttcatc gtcttcaaca gcgtcgacaa cgctgctgag gagcccttcg
     6241 cggcgccggt gcggcgtccg gagttcatcg gggcgcgtga cttcactccg gtgaggtgaa
     6301 ccggtgacgt cccgcttcac ccaggtggag caggcgcacc ggacgagccg ctgacagcgg
     6361 gctcatcacg caccggcgca cacggcgccc gggccacggg accgggggcg gccacaggcg
     6421 ggccgctccc ggtccggttg cgtttcacgg gccttctccg cccgtgcgcg ggtcagagcg
     6481 ccgggtagtc ggtgtacccc tcggccccgc cgccgtacat cgtggcgcgc cgggcctcgt
     6541 tgaacggccc cccggcacgg agccgcgcgg gtaggtcggg gttggccagg aagagctggc
     6601 cgtagctgac caggtcggcg gtgccctcct cgacgagggc cagcgcctcg ggaccggtcg
     6661 ggctgtcggc cgggttgagc atcagcgggc cgtcgaagag ctcgcgcagg cgcagggtga
     6721 gcgcccggcg ggcggggccg tcctcggcga ggtgcaggta ggcgaggccg agcggggcga
     6781 tccgggcgac cagcgcggtg tactccggct ccggctcggg ctcctcgatg ctgttgaagg
     6841 cgttgccggg cgagaggcgc agggcggtcc tggcggcgcc gatctcggcg acgaccgcct
     6901 cggtgacctc ggcggcgaac cggatgcggc cctcgaccga gccgccccag ccgtcggtgc
     6961 ggcggttgga gccgggggcg aggaactggt ggatcagata cccgttggcg ccgtgcagct
     7021 cgaccccgtc gaaaccggcg tcgaccgcgt tgcgggcggc cgtgacgtag tcggcgaggg
     7081 tggcgcggat ctcctcgtcg gtcagctcac gcggggtgac gaagtcctgc gggccctcgg
     7141 cggtgtacac ctggcccgtg gcggcgaccg cggagggggc gacgttgacc tcgccctccg
     7201 gcagcagcga ggggtggccg atccggccgg cgtgcatgag ctgggcgaag atccggccgc
     7261 ccgcggcgtg cacggcggag gtgaccgcgc gccaccccgt cacctgttcg gccgaatgga
     7321 ggccgggggt gtccgggtac ccctggccga cggcggaggg ctggatgccc tcggtgacga
     7381 tcagggcggc cgaggcgcgc tgcgcgtagt actcggccat gagcgccgtc ggcacgcccg
     7441 ccgcgccgga gcggctgcgg gtcatggggg ccatggcgat gcggtgcggg agcttggtgc
     7501 cggacaggtc gaggggctcg aaggcagtgc tcacgggcgg tctccagtgg ggggaacggg
     7561 cggttccggg cagagcgggg cccggccgac gcattgcatg gtcggccaat caatggggcc
     7621 gggagccact gtaacccatt gcttggccga ccaaaggtat agtcgggcga agaccgggac
     7681 gaggacgacc cagcgggaga cgtggatgaa cgaggcgacc ggccccgagc agaccccttg
     7741 tacctggccc gccgagcggg gcgcggcccc gcccggcggg cccgtcggcc atctcgtcac
     7801 ccgggtggcg cggctgcacc ggaccgccgc cggccgggcg ctgcgcgagt ccggtctcta
     7861 cccgggccag gagatcgtcc tcggccacct ctgggaggcg gacggcccgg tccgccagtc
     7921 cgacctgatc aaggcggtcg aactcgaccc ctccacggtg accaagaccc tgcagcggct
     7981 ggagcagtcc gggcacgtca cccgcgcccc cgaccccgcc gaccggcggg ccgtcctggt
     8041 cgaggcgacc gagctgagct gcgccctgag ccgccaggtc gagtccgcct gggccggcct
     8101 ggaggagcac gccctggccg gcctcacccc cgccgaccgc gacgaactgc tgcgcctgct
     8161 gacggcggtg gaggccaacc tctgcgaccg ggcgggcggc tgccccgagg cggagtagcc
     8221 cgcgcggctc agccgtcgag cgccgccacc agccggtcga cgtcgctctc ggtgttgtac
     8281 aggtggaagg cggcccgcag catcccggcc cggtcggaga cggtgatccc ccgctccccg
     8341 agggccgccg cccgctcccc cagccccggt acggagacga tggccgagcc gggggccggt
     8401 acgggggcgt gtccggcgcg ggtcagccgc tcccggaagc ggtcggcgag cgccacgtcg
     8461 tgggcgccca cggcctcggt cccgatccgc tccagcaggg cgagggcgtg ctgcgcgccg
     8521 gtccagacga gcggggcggg gctctcgtcg tagcggcgcg cgtcgggggc caggccggtg
     8581 acgggtccgt agcagctgtc gaacggggag tcgcccgcga cccagcccgc gaggagcggg
     8641 ggcagggcgc cgggctccgg cgggacggtg aggaaggcag tgcccttcgg ggcgagcagc
     8701 cacttgaacc cgacggccac cgtgtagtcg tacgcgccgg cctcgaaggt gtcccagccg
     8761 gcggcctggg aggcgtcgac cagggtgcgg gcgccgtggg cgcgggccgc ctcgcggacg
     8821 gcggcgaggt cggcgcggcg gccgtcggcg gactgcacgg cgctgaccgc gaccagggcg
     8881 gtcgtcccgg tgacgtgctc ggcgatccgc tccagcggca cggtccgtac gctcactccc
     8941 ggccggacgt ggaaggggtt gaccagcgag ctgaagtcgc cttcggcggt gagcacttcg
     9001 gcgccgtcgg gcagcgcggc ggcgatcagg ccggtgaagg tcgccaccga cgatccggtg
     9061 gcgacgcggg tggcggggac gcccaccagc cgggcgaagg aggcccgggc ggcgtcccgg
     9121 gcgccgaaca tgtcgggcgg ggtgccggcc gcggcggcgt cgaccgcctc gtgcacggcg
     9181 cgggccgcgc tcagcggcag gaggccggtg ccggcggtcg ccaggtgcgt ccgggagggc
     9241 ttgaactcgt cttcggccag gctgcgggcg aggtccatgc ggccactgtg caccctcgcg
     9301 cggcagccgt ccatggtgcg gcgaccgccg aaccgtccct ggtgggtacg ggtccggccc
     9361 gccgccccgc cggagcggat ggcgacgggc cgggcgggcg cggtcagttc ttcgggacct
     9421 cgcagccgtc ggggccgcac gcctcgccgc cctcggcgcc ggccgggatg gccagcccgg
     9481 aggcggggac gtggctctcc cacgcctggg tgagggcctg ggtgaagaca tcggcgggct
     9541 gggcgccgga gacgccgtac cgccggtcga ggacgaagaa cgggacgccg ctcgcgccca
     9601 gttcggtggc ggcgcgctcg tcggcgcgga cctcggcggc gtaccgctgt gcgtcggcga
     9661 ggacctcgcg ggcctcggtc tcctccagtc cggcctcgac ggcgagggcg acgagccgct
     9721 cgtcgtcgcc gaagacggtc gcggtctcgg cgaagttggc gcggtacagc acggcgagca
     9781 gttcgtcctg gcggccgcgc gccttggcga ggtggagcag ccggtgcatg tcgaaggtgc
     9841 tgccgtggtc gcggccctcg gtgaggtagg gcaggccctc ggcggcggcg ttctgcccga
     9901 ggttccgctc ggcctcggcg gcctgggcct cgctcatgcc gtacttcttg gcgagcatcg
     9961 gcaggaccag ctcggggtcg cccttggggc gcgccgggtc cagctcgaag gagcggtgca
    10021 cgacctcgat ccggtcgcgg tgcgggaagg cggcgagcgc cttgtcgaac cgcgcctttc
    10081 ctacgtagca ccagggacag gcgatgtcgg accagatctc gacgcgcatg gctgcggttc
    10141 aactcctcgg cccggggacc gggcaggtcg ggggtgccgg gagcggtctc ccggggcgtg
    10201 cggacaccag ggtacggagg gtgcctccgc tctgccgcaa cgcgggggcg gaccggtgca
    10261 ttccggaccg gcccgggtca ccccggcctc cgcctcaggc cccgcgttcg gcccgcccgt
    10321 ccacgtgccg gggcagcccc agcgggttgc cgtcccgcag ctccggcggc agcagcgcct
    10381 cgggggtggt ctggtacgc
//
