LOCUS       NZ_VOGX01000018        10216 bp    DNA     linear   CON 15-OCT-2024
DEFINITION  Streptomyces albidoflavus strain 77 scaffold11_cov50_1_2691061,
            whole genome shotgun sequence.
ACCESSION   NZ_VOGX01000018
VERSION     NZ_VOGX01000018.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces albidoflavus
  ORGANISM  Streptomyces albidoflavus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces albidoflavus group.
REFERENCE   1  (bases 1 to 2691061)
  AUTHORS   Pylro,V., Dias,A., Andreote,F., Varani,A., Andreote,C., Bernardo,E.
            and Martins,T.
  TITLE     Draft Genomic Sequences of Streptomyces misionensis and Streptomyces
            albidoflavus, bacteria applied for phytopathogen biocontrol
  JOURNAL   Microbiol Resour Announc (2019) In press
REFERENCE   2  (bases 1 to 2691061)
  AUTHORS   Pylro,V., Dias,A., Andreote,F., Varani,A., Andreote,C., Bernardo,E.
            and Martins,T.
  TITLE     Draft Genomic Sequences of Streptomyces misionensis and Streptomyces
            albidoflavus, bacteria applied for phytopathogen biocontrol
  JOURNAL   Microbiol Resour Announc (2019) In press
REFERENCE   3  (bases 1 to 2691061)
  AUTHORS   Pylro,V., Dias,A., Andreote,F., Varani,A., Andreote,C., Bernardo,E.
            and Martins,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2019) Biology, UFLA, Universidade Federal de
            Lavras, Lavras, MG 37200000, Brazil
REFERENCE   4  (bases 1 to 2691061)
  AUTHORS   Pylro,V., Dias,A., Andreote,F., Varani,A., Andreote,C., Bernardo,E.
            and Martins,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JUL-2019) Biology, UFLA, Universidade Federal de
            Lavras, Lavras, MG 37200000, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-JUL-2019
            Assembly Method                   :: SPAdes v. 3.12
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 36.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007896905.1-RS_2024_10_13
            Annotation Date                   :: 10/13/2024 04:20:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,551
            CDSs (total)                      :: 6,456
            Genes (coding)                    :: 6,343
            CDSs (with protein)               :: 6,343
            Genes (RNA)                       :: 95
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 5 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 34 of 113
            Pseudo Genes (incomplete)         :: 81 of 113
            Pseudo Genes (internal stop)      :: 8 of 113
            Pseudo Genes (multiple problems)  :: 8 of 113
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 03:41:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 684413
            Orig. end                         :: 694629
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VOGX01000018.1.
            Bacteria and source DNA available from MEB Lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(556..565)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            679..1578
                     /locus_tag="FRZ02_RS05365"
                     /old_locus_tag="FRZ02_05365"
     CDS             679..1578
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506775.1"
                     /locus_tag="FRZ02_RS05365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_05365"
                     /product="hypothetical protein"
                     /protein_id="WP_261399545.1"
                     /transl_table=11
                     /translation="MRTVLRRSRSVPSRRPRGRVAVGAALVAAALFTAAPPALADTSPS
                     PSVSQDEEAEAGPTEAGTAFRTAARLAQGQRGTASASTGDYLYWVFPADIGQRPTVRAT
                     VSLPAEQARQSASTWRIDVYDGLRRRQPCTYGLQERRAAEDAAEVRLTCTLRTVRAYSE
                     PWANDPLPGAYYVRLTVSRLAADDLGRPFQAEVAVDSQETGGSTAVGGRLSEPLTPGVS
                     AVSAEEQAEEETAEEDGAEQAPRPAALAEPEDGWSSGWWTDRWLWTAGGAVLAALAGVF
                     GYTLTRGNGRPGRVPPGV"
     gene            1769..3586
                     /locus_tag="FRZ02_RS05370"
                     /old_locus_tag="FRZ02_05370"
     CDS             1769..3586
                     /NRPS_PKS="Domain: AMP-binding (23-474). E-value: 6e-89.
                     Score: 290.4. Matches aSDomain:
                     nrpspksdomains_FRZ02_RS05370_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412454.1"
                     /locus_tag="FRZ02_RS05370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_05370"
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="WP_146580170.1"
                     /sec_met_domain="AMP-binding (E-value: 4.8e-84, bitscore:
                     279.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREVSVERLVAPPVSGGLADSVFHTAATYPTRPQLALEPTKPGGP
                     WRTVTAARLRDEVLALARGLLAEGVQPGDRVVLMSRTRPEWTLLAYALWTLGAEVVPVY
                     PTSSIEQVRWVLADAGVRAAVVEHSGQAMTIAAACADPGALLGLWQLDLDCTAELAERG
                     AEVPEDEVLRLRAAVRPEATAVICYTSGTTGAAKGCVISHANLAAECDTLIAGWGHLLA
                     PRGEQPSVLAFLPLAHCYGLMVVVTCVRGGYLLAHQPEMAPAVLLPALASFRPTFLFAV
                     PYIFERIFDEACREAEAAGHGVIFEKARAAAVAHAEAREKHTAGTGHGPGPVTRLRHSV
                     YERAVYAKVRAVFGGRVRSAVSGGSPLRRELGLFFQGAGVTVYDGYGLTETTAAVTAQP
                     PGRVRFGTVGRPLPGSTVRIAGDGEVWVRGDTVFRGYLHDPPGLADGWLATGDTGRFDP
                     DGYLVITGRKKDIIITSGGKSVSPQLLEEQLRRHPLIAQCLVVGDNRPYVAALLTVDQE
                     ALAHWQRLRGASGRAASAPADDTELRAQLQRAVNQANTRVSRAESIRAFEILPVDFTRQ
                     EGLMTPSFKLRRSAVTDTYAEVIEALYAR"
     aSDomain        1838..3190
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FRZ02_RS05370_AMP-binding.1"
                     /evalue="6.00E-89"
                     /label="FRZ02_RS05370_AMP-binding.1"
                     /locus_tag="FRZ02_RS05370"
                     /protein_end="474"
                     /protein_start="23"
                     /score="290.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="HTAATYPTRPQLALEPTKPGGPWRTVTAARLRDEVLALARGLLAE
                     GVQPGDRVVLMSRTRPEWTLLAYALWTLGAEVVPVYPTSSIEQVRWVLADAGVRAAVVE
                     HSGQAMTIAAACADPGALLGLWQLDLDCTAELAERGAEVPEDEVLRLRAAVRPEATAVI
                     CYTSGTTGAAKGCVISHANLAAECDTLIAGWGHLLAPRGEQPSVLAFLPLAHCYGLMVV
                     VTCVRGGYLLAHQPEMAPAVLLPALASFRPTFLFAVPYIFERIFDEACREAEAAGHGVI
                     FEKARAAAVAHAEAREKHTAGTGHGPGPVTRLRHSVYERAVYAKVRAVFGGRVRSAVSG
                     GSPLRRELGLFFQGAGVTVYDGYGLTETTAAVTAQPPGRVRFGTVGRPLPGSTVRIAGD
                     GEVWVRGDTVFRGYLHDPPGLADGWLATGDTGRFDPDGYLVITGRKKDIIIT"
     CDS_motif       2318..2377
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FRZ02_RS05370_0001"
                     /evalue="1.10E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="FRZ02_RS05370"
                     /protein_end="203"
                     /protein_start="183"
                     /score="21.2"
                     /tool="antismash"
                     /translation="AVICYTSGTTGAAKGCVISH"
     CDS_motif       3035..3112
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FRZ02_RS05370_0002"
                     /evalue="8.10E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="FRZ02_RS05370"
                     /protein_end="448"
                     /protein_start="422"
                     /score="18.3"
                     /tool="antismash"
                     /translation="GEVWVRGDTVFRGYLHDPPGLADGWL"
     gene            3810..4316
                     /locus_tag="FRZ02_RS05375"
                     /old_locus_tag="FRZ02_05375"
     CDS             3810..4316
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640757.1"
                     /locus_tag="FRZ02_RS05375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_05375"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_093845782.1"
                     /transl_table=11
                     /translation="MRIRAVHQAELTALQDIERAAGVWFREIGMAEIADDEPPPVAELA
                     RYARSGLAWVAADGEDRPVAYLVAERVDGNLHVEQVTVRPESARRGVGRALLDHAAGRA
                     RSEGAAALTLTTFTEVPWNAPYYVRCGFRPLAERELTPGLREIRRAEAAHGLDRWPRCC
                     MRREV"
     misc_feature    complement(4516..4533)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.61"
                     /tool="antismash"
     misc_feature    4899..4914
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 16.77"
                     /tool="antismash"
     misc_feature    4951..4971
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.44"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="FRZ02_RS05380"
                     /old_locus_tag="FRZ02_05380"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="FRZ02_RS05380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_05380"
                     /product="hypothetical protein"
                     /protein_id="WP_008412458.1"
                     /sec_met_domain="strepbact (E-value: 1.6e-37, bitscore:
                     125.0, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYTPQVETAEIADSELDNISGGLVNGLVGSVTSSLDSVAPVSDT
                     LGTVTGVVEQTTGLNTAPVTGLVAGL"
     misc_feature    5015..5030
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.07"
                     /tool="antismash"
     gene            5414..6220
                     /locus_tag="FRZ02_RS05385"
                     /old_locus_tag="FRZ02_05385"
     CDS             5414..6220
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640761.1"
                     /locus_tag="FRZ02_RS05385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_05385"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_146580172.1"
                     /transl_table=11
                     /translation="MRFRQQALTKLQSPEQLDLPVRFARPQGLLVLAVTLVVMAAGGVW
                     AVTGSVATTLTAPGVLTHAQGSYVVQSTVSGQVTAVRTAEGRRVARGTPLLTVRTARGD
                     VTIRALAAGRVTALAAATGAVVTTGSDLAAVERQENPDDPLLAVLYAPADSGPSLRPGA
                     EVDLTVQSVPAQSYGVLRGTVKSVGRAARTRQQISADLGSTQLGEEFSRNGPPIAVEVR
                     LHPTKTTRSGYTWSTSDGPPQPLTTLTLVDGAVHLDEQRPADWLLP"
     gene            6217..8514
                     /locus_tag="FRZ02_RS05390"
                     /old_locus_tag="FRZ02_05390"
     CDS             6217..8514
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640763.1"
                     /locus_tag="FRZ02_RS05390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FRZ02_05390"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_146580174.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.7e-36, bitscore:
                     121.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPADAPAAGTGSTAPPTPGGRRGRRAAPSSGRRRAAPPRRTPR
                     RRPVRTPTVLQMEAVECGAACLAMVLAHHGRHVPLEELRIACGVSRDGSRASNVLKAAR
                     SYGLTAKGMQMEPAALREVRAPAVLFWEFNHYVVHEGFLRRPGRRGAVRLNDPDKGRRV
                     VSAEEFDASFTGVALVLEPGPSFRKGGRKPGLLGALPARLRGTGATLLTALLASLLLVV
                     VGAALPALSRTYIDLFLIGERTSVLGPLFAAMGALVALTASLTALQQANLLRGRIISST
                     LSSARFLRHLLRLPVTFFAQRSPADLVQRLQSNDTVAETLARDLAAAAVDGVVVVLYAL
                     LLWTYDPQLTVVGVGIALLNIVAMRVVIHLRATRTQKLRADNARLTTTAYTGLQLIETM
                     KATGGEDGFFRRWAGQHATTLEEQQRLGVPTAVLSVVAPALATFNSALILWIGGLRAVE
                     GHLSVGLLVAFQALMVRFTAPLTRLNGVAGRIQDFAADVTRLRDVEAFPADGLYRREGA
                     GGGEADTRRLAGRVELEGLTFGYSPLDAPLLTEFSLTVGPGRQVALVGGSGSGKSTVSR
                     LLAGLYQPWEGTIRIDGQPLTEIPRSALAASVSFVDQDIFLFEGTVRENVTLWDPSLPD
                     EAVRTALRDAGLEEVIARRPDGIHARVEQDGRNFSGGQRQRLEIARALVRRPSVLVLDE
                     VTSALDAETEQRVMAALRRRGCACVIIAHRLSTVRDSDEIVVLDGGRVVERGRHAALVA
                     RGGAYAALVEEK"
ORIGIN
        1 gggagcgacc ggcggcacct acaccgccgt gcagcacaag gaggacctct ccgacaaggt
       61 gaagcagctg gtggaccgcg cggccgaccc ggtagtcacg ccggtgccgg tcgagggggc
      121 cgcggcctgc gccgacgcgc cgaagatcga gccgggcctg tacaccgacc gccagccgct
      181 cggcacccac cgctggtacc gggtggacgt gctgcccggc caggagctgc gcgcctcggt
      241 gagtgtggcg gccgaccgcc cgctcgccga cgactacggc gtgctgctgc gcgcggtgac
      301 cgcgcacggc cgcgagctgg tgcgcggctc ggaggcgggc agcggccgga ccgacgtgat
      361 ctcgtccggt ctgcgccacc cggagcggga gcgggaggac gccgacaccg acaagccggc
      421 cgcgaccccg gtctgcctgg aggtcagcaa ctccttcgcc ccggcctccg ccaaggccgc
      481 cgacgcctcg gcgccgggta tgccgctgga gctgacggtc gacgtggtcg acgggccgtc
      541 cgacgcctcg gacacggccg ccttcgggct gggccgtggc tggtggctgc tgggcgtgct
      601 ggtgctcgcc ggtttcctcg gcggcctgat cttcggctgg ctgtcgcgct ggcgcttcgc
      661 cgtctggagg acccactgat gcgtaccgtt cttcgcaggt cccggtccgt gccgtcccgg
      721 cgcccgcgcg ggcgcgtcgc cgtgggggcc gccctcgtcg cggcggcgct gttcacggcc
      781 gcgccgccgg ccctcgccga cacctcgccc tcgccctccg tctcgcagga cgaggaggcc
      841 gaggccggtc cgaccgaggc gggcaccgcg ttccgcacgg cggcgcggct cgcgcagggg
      901 cagcgcggca ccgcctccgc gtcgaccggt gactacctct actgggtctt cccggccgac
      961 atcgggcagc gcccgaccgt gcgggcgacc gtgtcgctcc ccgccgaaca ggcccggcag
     1021 tccgcctcca cctggcggat cgacgtctac gacgggctcc gccgccgcca gccgtgcacc
     1081 tacggcctcc aggagcggcg ggccgccgag gacgccgccg aggtgcggct gacctgcacg
     1141 ctccgtaccg tgcgggccta ctccgagccg tgggccaacg acccgctgcc cggggcgtac
     1201 tacgtccggc tgacggtctc ccggctcgcc gccgacgacc tgggccggcc gttccaggcc
     1261 gaggtggcgg tcgactccca ggagaccggc ggctccacgg cggtcggcgg gaggctctcc
     1321 gagccgctga cgcccggcgt ctcggcggtc tccgccgagg agcaggccga ggaggagacg
     1381 gccgaggagg acggtgccga gcaggcgccg cgtccggcgg cgctcgccga gcccgaggac
     1441 ggctggtcct ccggctggtg gaccgaccgg tggctgtgga cggccggggg tgccgtactg
     1501 gccgccctgg cgggcgtctt cggctacacc ctgacccggg gcaacgggcg tcccggccgg
     1561 gtgccgcccg gcgtctgacg ggccgacggc tccgcgcgtc cccggccggg cggcgcgcgg
     1621 agccgccccc ggcgcacggt ggtgaactcc cccgtcaccc ctggccagtg cgtgcggggc
     1681 gctgtaggtt gccgagccgg ccatccgtca ggtacgggcc ccgcgctggt gcggcacgcg
     1741 gccgggatga cgaacggagg cgtacccggt gcgggaagtg agtgtggagc gcctggtcgc
     1801 cccgccggtc tccggcggcc tggccgactc ggtcttccac accgccgcga cgtacccgac
     1861 gcgcccccag ctggccctgg aacccacaaa gcccggcggc ccctggcgca ccgtcacggc
     1921 ggcccggctc cgcgacgagg tactggcgct ggcccggggc ctgctggccg agggggtgca
     1981 gcccggcgac cgggtggtgc tgatgtcccg tacccggccc gagtggacgc tgctggcgta
     2041 cgccctgtgg acgctgggcg ccgaggtggt gccggtctac cccacctcct ccatcgagca
     2101 ggtccgctgg gtcctcgccg acgcgggcgt ccgggcggcg gtggtggagc acagcgggca
     2161 ggcgatgacg atcgcggcgg cctgcgccga ccccggcgcc ctgctcgggc tctggcagct
     2221 ggacctggac tgcacggcgg aactcgccga gcgcggcgcc gaggtgccgg aggacgaggt
     2281 gctgcggctg cgggcggcgg tccgcccgga ggcgaccgcc gtcatctgct acacctcggg
     2341 caccaccggc gccgccaagg gctgcgtgat cagccacgcc aacctggccg ccgagtgcga
     2401 caccctcatc gcgggctggg gccacctgct ggccccccgc ggcgaacagc cctcggtcct
     2461 cgccttcctg ccgctggccc actgctacgg cctgatggtg gtggtcacct gtgtacgcgg
     2521 cggctatctc ctcgcccacc agccggagat ggccccggcg gtgctcctcc cggccctcgc
     2581 ctcgttccgg cccaccttcc tcttcgccgt cccctacatc ttcgagcgga tcttcgacga
     2641 ggcgtgccgg gaggccgagg ccgccgggca cggggtgatc ttcgagaagg cgcgggcggc
     2701 cgccgtggcc cacgccgagg cccgcgagaa gcacacggcg ggcaccggac acggcccggg
     2761 accggtcacc cggctgcggc actccgtcta cgagcgggcc gtctacgcca aggtccgggc
     2821 ggtcttcggc ggacgggtcc gctcggccgt ctcgggcggc tcgcccctcc gccgcgaact
     2881 ggggctcttc ttccagggcg cgggggtgac cgtctacgac ggctacgggc tgaccgagac
     2941 cacggcggcg gtgaccgccc agcccccggg ccgggtccgc ttcggcaccg tggggcgccc
     3001 gctgccgggc tcgaccgtcc ggatcgccgg cgacggcgag gtgtgggtac gcggggacac
     3061 ggtcttccgg ggctatctgc acgacccgcc ggggctcgcc gacggctggc tggccaccgg
     3121 ggacaccggc cgcttcgacc cggacggcta cctggtgatc accggccgca agaaggacat
     3181 catcatcacc agcggcggca agagcgtctc cccacaactg ctggaggagc agttgcgccg
     3241 ccatccgctg atcgcccagt gcctggtggt cggcgacaac cggccctacg tggcggccct
     3301 gctcaccgtc gaccaggagg ccctggccca ctggcagcgc ctgcgcgggg cgtccggccg
     3361 ggccgcctcg gccccggcgg acgacacgga gctgcgggcc cagctccagc gggcggtcaa
     3421 ccaggccaac acccgggtct cacgcgccga gtcgatccgg gccttcgaga tcctgccggt
     3481 cgacttcacc cgtcaggagg gcctgatgac cccctcgttc aaactccgcc gcagcgccgt
     3541 cacggacacc tacgcggagg tcatcgaggc cctgtacgcc cgctgaacgg ccgtgcgggg
     3601 cgggtcgcga ggaccccgcg cgaagaattg cccgcccccg gccggcatga ctccccccgc
     3661 cccgggcaca cgcccctcag aacgacctga cggcccttcc ccctggcctg gcaggttgcc
     3721 tcggccccac cggactttcc ccccttcgtc cggtggggcc gccccctttt cccgttgcct
     3781 cccgcccccg cgcaccggac catgccccca tgcgtatccg agccgtgcac caggccgaac
     3841 tgacggccct ccaggacatc gaacgggccg ccggagtctg gttccgggag atcggcatgg
     3901 ccgagatcgc cgacgacgag ccgccgccgg tggcggagct ggcgcggtac gcacggtcgg
     3961 ggctcgcctg ggtggcggcc gacggggagg accggcccgt ggcgtacctg gtcgcggagc
     4021 gggtcgacgg caaccttcac gtcgagcagg tgacggtgcg tccggagagt gcgcgccggg
     4081 gtgtcgggcg ggccctgctg gatcacgcgg ccgggcgggc ccggagcgag ggggccgcgg
     4141 cgttgacgct caccaccttc accgaggtgc cgtggaacgc gccgtactac gtgcggtgcg
     4201 gcttccgccc gctggcggag cgcgagctga cgccggggct gcgggagatc cggcgggccg
     4261 aggcggcgca cggactggac cggtggccgc gctgctgcat gcgccgggag gtgtgagcgc
     4321 gggcgccgag gagaacgcgg ggcgcgaaag ggcggggacg gggaagcgcg cattccccgt
     4381 ccccgccctt tcgcgccctg tgggcaattc ccgcccctgc ccctccgctc tcttttctca
     4441 gtgcgcaaga attcgccggg cgaaggcaat caggccgctt ccgctccttc ttctcctcgt
     4501 ggcggacgct gttcctccac atgttggaaa catggccttc tcagagccgt ccgcacacga
     4561 atccgccgca ccccgcagcg ggttctcctg cggtgaatct gcacagccgt gccgcagctc
     4621 cgtcaccgcc acccatacct tcaccgggcg tgaacggccg aggtcgacgg cgtgcaccag
     4681 ggcttcacag cgaggggcgc ggtgtacacg cgagcgtgac cccacctggt acggcggcgg
     4741 gacggtgtga aggtggtgcc gttctcctgt gcccgattcc gcacacggtg gtgaggagaa
     4801 cggcaccgcc ggccgaattc cgctctcacc gattggccgc cccgcgcacc gtcgtgcttt
     4861 gatccatcgc actgcggcac cgcccccgga tctgaaggtt cacgcctttc acccgggggc
     4921 cgtcagaaga ttttcccctt cttctgcacg agtgcgtcgc ggtgactatt tccgtcagtg
     4981 atgcgaaggg aactcccccc atgagctaca ccccccaggt cgagaccgcc gagatcgccg
     5041 acagcgagct ggacaacatc tccggcggcc tcgtgaacgg cctcgtcggc tcggtcacct
     5101 ccagcctcga ctcggtcgcc ccggtctccg acaccctcgg caccgtcacc ggcgtcgtgg
     5161 agcagaccac cggtctgaac accgccccgg tcaccggcct cgtcgccggt ctctgagacc
     5221 ccacgcgtcg cctgacgacg cgtgcggccc gctgaggcgg accgctcggc cggtgccccg
     5281 gacccgctcg ggttccgggg caccggccgc ctcctttgtc ctccccggac gcccgcgcgg
     5341 cgtccccccg caccacggcg acacccggcc gcacggccga gaggccggtg cccggcagtc
     5401 gaggaagtct tccgtgcgct ttcgccaaca ggccctgacc aagctccagt cgcccgaaca
     5461 gctggacctg ccggtgcggt tcgcccgccc gcagggactc ctggtcctcg ccgtcaccct
     5521 cgtcgtgatg gccgccggcg gcgtgtgggc cgtcaccggc tcggtcgcca ccaccctcac
     5581 cgcgcccggc gtcctcaccc acgcccaggg cagctacgtc gtccagtcca ccgtctccgg
     5641 acaggtcacc gccgtccgca ccgccgaggg ccgccgcgtc gcccgcggca ccccgctgct
     5701 gaccgtccgc accgcgcggg gcgacgtgac catccgcgcg ctggccgccg ggcgggtcac
     5761 cgcactggcc gccgccaccg gcgccgtcgt caccaccggc tccgacctgg ccgccgtcga
     5821 gcgccaggag aacccggacg accccctcct cgcggtgctc tacgcccccg ccgacagcgg
     5881 cccctccctc cggcccggcg ccgaggtcga cctcaccgtg cagtcggtgc ccgcccagag
     5941 ctacggcgtg ctgcgcggca ccgtgaagtc ggtcggccgc gccgcccgga cccggcagca
     6001 gatctccgcc gacctcggct ccacccagct cggcgaggag ttctcccgga acgggccgcc
     6061 gatcgccgtc gaggtccggc tgcaccccac gaagaccacc cgcagcggct acacctggtc
     6121 gacctccgac ggcccgccgc agccgctgac caccctcacc ctcgtcgacg gcgccgtcca
     6181 cctggacgaa cagcgcccgg ccgattggct cctcccgtga ccgcacccgc cgacgccccc
     6241 gccgccggga ccggctccac cgccccgccc acccccggcg gccgccgggg ccgccgcgcc
     6301 gccccctcct ccggccgccg ccgcgccgcg cccccgcgcc gtacgccccg gcggcggccc
     6361 gtccgcaccc ccaccgtcct gcagatggag gcggtggagt gcggcgccgc ctgtctcgcc
     6421 atggttctcg cccaccacgg gcggcacgtc ccgctggagg agctgcggat cgcctgcggc
     6481 gtctcccgcg acggctcacg cgccagcaac gtcctgaagg cggcccgcag ttacggcctg
     6541 accgccaagg gcatgcagat ggaaccggcc gccctgcgcg aggtgcgggc ccccgccgtg
     6601 ctgttctggg agttcaacca ctacgtcgtc cacgaggggt tcctgcggcg tcccgggcgg
     6661 cgcggcgcgg tccgcctcaa cgaccccgac aaggggcgcc gggtggtgag cgccgaggag
     6721 ttcgacgcct cgttcaccgg cgtcgccctg gtcctggagc cgggcccctc gttccgcaag
     6781 ggcggccgca agcccgggct gctcggcgcg ctgccggccc ggctgcgcgg caccggcgcc
     6841 accctgctga ccgcgctcct ggccagcctg ctgctggtcg tggtgggcgc ggcgctgccc
     6901 gcgctgagcc ggacgtacat cgacctgttc ctgatcgggg agcggacctc ggtgctcggg
     6961 ccgctcttcg cggcgatggg cgcgctggtg gcgctgaccg cctcgctgac cgccctccag
     7021 caggccaatc tgctgcgcgg gcggatcatc tcctcgacgc tgagcagcgc ccggttcctg
     7081 cgccatctgc tgcggctgcc ggtgaccttc ttcgcccagc gctccccggc cgacctggtg
     7141 cagcggctcc agtcgaacga cacggtggcc gagaccctcg cccgcgacct ggcggccgcc
     7201 gccgtcgacg gggtggtggt ggtgctgtac gcgctgctgc tgtggacgta cgacccgcaa
     7261 ctgaccgtgg tcggggtggg gatcgcgctg ctcaacatcg tggcgatgcg ggtggtgatc
     7321 cacctgcggg ccacccggac gcagaagctg cgggccgaca acgcgcggct caccaccacc
     7381 gcgtacaccg ggctccagct catcgagacg atgaaggcga ccggcggcga ggacgggttc
     7441 ttccgcaggt gggcggggca gcacgcgacg acgctggagg agcagcagcg gctcggggtg
     7501 ccgacggcgg tgctgtcggt ggtggccccg gcgctcgcca ccttcaacag cgcgctgatc
     7561 ctgtggatcg gcgggctgcg ggcggtcgag gggcatctgt cggtcggact gctggtggcc
     7621 ttccaggcgc tgatggtgcg gttcaccgcg ccgctgaccc ggctcaacgg cgtcgcgggc
     7681 cgcatccagg acttcgcggc cgacgtgacc cggctgcggg acgtcgaggc gttcccggcg
     7741 gacgggctgt accggcggga gggggccggc ggcggcgagg cggacacccg gcggctggcg
     7801 ggccgggtgg agctggaagg gctgaccttc ggttacagcc cgctggacgc accgctgctg
     7861 acggagttct cgctgacggt cgggccgggg cggcaggtgg cgctggtcgg cgggtcgggc
     7921 agcggcaagt cgacggtctc ccggctgctc gcgggcctgt accagccgtg ggagggcacc
     7981 atccgcatcg acgggcagcc gctcaccgag atcccgcgct ccgcgctggc cgcctcggtc
     8041 tccttcgtcg accaggacat cttcctcttc gagggcacgg tgcgggagaa cgtcacgctc
     8101 tgggacccct cgctgcccga cgaggcggtc cgtaccgcgc tgcgcgacgc cgggctggag
     8161 gaggtgatcg cccgccgccc cgacggcatc cacgcccggg tcgagcagga cgggcggaac
     8221 ttctccggcg gccagcggca gcggctggag atcgcccggg ccctggtgcg ccgccccagc
     8281 gtgctggtgc tggacgaggt gaccagcgcg ctggacgcgg agaccgagca gcgggtgatg
     8341 gcggcgctca ggcggcgcgg ctgcgcctgc gtgatcatcg cgcaccgcct ctccaccgtc
     8401 cgcgacagcg acgagatcgt ggtgctggac ggcggccggg tcgtggaacg cggccgccac
     8461 gccgcactgg tcgcccgggg cggcgcgtac gcggccctgg tcgaggagaa gtgagatgac
     8521 gggcacccag caggcttccc accccacccc ggccgccgac ccggtgacgg cggcgctcgg
     8581 cggactcggc gcaccggtgg agggcgccgc agggtcggca ctcctgctcg gcggcccctc
     8641 ggcgctgtgg ctggtcaccg agggcagcct cgacctcttc gcggtggcgg ccgccgagga
     8701 cggccactgg cactacctcg gccgcctgga ggccggcacg ctcctgctcg gcgccgtcga
     8761 gggcccccgc cacaccctgg tgggccgcct ctcctcccgc tgcgccgtac gccgcctgcc
     8821 gctgcgcgag ctgtacgcgc cgcagggggc cgaggggtac cccgggtacg agccggtcgc
     8881 cgggccgctg gagcacgcgg tggggctcgg cctcggccgg gggctcgccg tgctgttcgc
     8941 ggcaccgcag gcggacgggc ccgcggcggg gtccgggccc gccgacgagg acgtgctgtg
     9001 ggtggacgtc ccgccgggca gtgtccggct gggggccgag ttcgccgccg aggccgcggc
     9061 cgacctgctg gtcgacgggg cgctgtggca gtccctggtg gaccagcagt accgcctgct
     9121 gaccgccgtc gacggctggg tggagcggct ggagcgggcc cacgaggacc gggcggcggc
     9181 cggtatccgc gccggtgagg cggtacggga gcgggccgac caggcgctgc tcgactcgat
     9241 cggccgccgc gaccgctccc cgagggcgcg cggcgaggcg ggcgacgacg cggtgtacgc
     9301 ggtcttccgc caggtcgcgc gggcgagcgg catcgcgctg ccggaggcgg cggcgcgttc
     9361 ggtcgcgggc ggcgggcggc tcgacccggt ggagcggatg gccgccgccg cccggctgcg
     9421 gacccgcccg gtccggctgg ccggcgagtg gtggcgtacc gacgccgggc cgctggtggg
     9481 gcggcgcgcc aggtccggcg cgccggtggc cctgctctgg cggcgcggcc ggtacgaggc
     9541 gctcaacccg gcgaccggcg cccggatcag gatcggcgcc aaggaggcgg ccgagttcga
     9601 ggaacgcgcc accatgctct accggccgct tcccgagggc ccgctgacgc tgcgccggct
     9661 gatgttcttc agcctgcgcg gctcccgggg cgacctgcgc aacctcgtcc tgagcggcct
     9721 ggtcaccgtg gcgctgggcg cgctggtgcc ggtggcgacc gggcaggtgc tcggcgcgta
     9781 cgtgcccagt gcgcagaccg ggctgatcgc gcagatgtgt gtggcggtga tggtggccgg
     9841 ggtggtctcg gcggcgttca tgctgctcca gtcgttgacg ctgctgcgtc tggaggggcg
     9901 gatcgaggcg gcgttgcagc cggccgtctg ggaccggctg ctgcggctgc cgacggcctt
     9961 cttcgcggac cgctccaccg gtgagctggc cagtgccgcg atggggatca gcgcgatccg
    10021 ccgggtgctg tcggggatcg ggccgaccgc cgtgcagtcg gtgacggtgg ccggggtcaa
    10081 cctggtgctg ctgctggtgc acagcgtgcc gatggcgctg gcggcgatcg gcgtgctgct
    10141 ggtggtctcg gtggtcttcc tgctgctggg gctctggcag gtgaagtggc agcgcaagct
    10201 ggtggagctg ggcaac
//
