LOCUS       NZ_CP031605            12151 bp    DNA     circular CON 21-DEC-2024
DEFINITION  Staphylococcus pseudintermedius strain VB16 chromosome, complete
            genome.
ACCESSION   NZ_CP031605
VERSION     NZ_CP031605.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 2791609)
  AUTHORS   Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2018) Microbiology, Veterinary Science,
            Chulalongkorn University, Henry-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-FEB-2018
            Assembly Method                   :: Canu v. 1.3
            Assembly Name                     :: VB16
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Oxford Nanopore Technologies
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007833695.1-RS_2024_12_21
            Annotation Date                   :: 12/21/2024 01:51:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,737
            CDSs (total)                      :: 2,655
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 37 of 84
            Pseudo Genes (incomplete)         :: 50 of 84
            Pseudo Genes (internal stop)      :: 12 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:07:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2450092
            Orig. end                         :: 2462243
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031605.1.
            Bacteria and source DNA available from Nuvee prapasarakul,
            Henri-Dunant Rd., Pathumwan, Bangkok 10330, Thailand.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12151
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12151
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12151
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7151](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7151)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(168..1949)
                     /gene="recQ"
                     /locus_tag="DRC71_RS11690"
                     /old_locus_tag="DRC71_11775"
     CDS             complement(168..1949)
                     /EC_number="5.6.2.4"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424488.1"
                     /locus_tag="DRC71_RS11690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_11775"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_015728744.1"
                     /transl_table=11
                     /translation="MEQTLSHYFGYKSFRPGQKEIIERVLNHHHTLGVLPTGGGKSLCY
                     QVPGLMFKGTTIVISPLISLMKDQVDQLTAMGISAAYLNSSLSTQAQKEIETQLVNGEL
                     KFLYIAPERFEQTYFEMLLRRVDISLVAFDEAHCISKWGHDFRPSYQAVIQRVLTMPQQ
                     FSIVALTATATTEVQQDIMERLMISRQHLVKTSIRRRNLKFQVNGTYQRQKFVLDYIQK
                     HSQKAGIVYCSTRKQVEALYEALEDADIPVSYYHAGMTAKQRDEAQNDFLYDRTRVVVA
                     TNAFGMGIDKSNVRYVIHYNMPGDLESYYQEAGRAGRDGLDSDCILLYSDRDIALHQYF
                     IGASKADDDYKERMGEKLTKMIQYTKTTKCLEATLVHYFEPNEKLEECHQCSNCIDKNK
                     TYNMTKEAKMIISGVARLRQQEGYQTVIQVLRGEVSDYIRHQGYENLSTYGLMKDYTTG
                     ELHHLIDELRFKGFLNEHDEILMCDASVEKLLSEDTQVFTRPFKRKSKETVHINTVEGV
                     DRTLFEKLVEVRREMSERLNMPPTNIFTDYTLEAFAKRKPVTKQEMIQIDGVGSYKLKH
                     YCPEFLEAIQEYKMHRS"
     gene            complement(1959..3817)
                     /locus_tag="DRC71_RS11695"
                     /old_locus_tag="DRC71_11780"
                     /pseudo=""
     CDS             complement(1959..3817)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002496151.1"
                     /locus_tag="DRC71_RS11695"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_11780"
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEAYKIERLNKSYADKVIFDDLQLSISNQERIGLVGINGTGKSSL
                     LKVIAGMDEDFDAVQSHPKQYRIRYAAQNHNLEPTLTVYEAVYSADSKELQVIKRYEEA
                     TAQYAEQQTDKAFDAMMAAQADMDAYSAWDYSAEIKTILSKLGIHDTTRRMSELSGGQQ
                     KRVVLARTLIEKPDLLLLDEPTNHLDFESIHWLIQFVKSYPKTVVFVTHDRYFLNEVAT
                     RIVELRNGKLDTYPGNYEAYIAMRAEREEIEVRQQTKQRALFKQELAWMRAGVKAQGKI
                     VFMT"
     gene            3929..4744
                     /locus_tag="DRC71_RS11700"
                     /old_locus_tag="DRC71_11785"
     CDS             3929..4744
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168536.1"
                     /locus_tag="DRC71_RS11700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_11785"
                     /product="ZIP family metal transporter"
                     /protein_id="WP_015728742.1"
                     /transl_table=11
                     /translation="MLDYISNMPPFLQALLAGVITWLLTALGAATVFVFKSVNEKVLNS
                     MQGFAAGIMIAASFWSLLQPAIESSSGSTVPWLPAAIGFLLGGVFIRSLDYVIPHMHRN
                     ATDESQHQEGVETSLNKNTLLLLAITLHNIPEGLAIGVAFGGIATGNEHATLLGALGLA
                     IGIGIQNIPEGAALSLPIHASGQSKWKAFNYGQASALVEPVFATVGAAAVLVVTPILPY
                     ALAFAAGAMIFVVVEELIPDSQAGKNTDLATLSLMVGFTIMMILDVALG"
     gene            complement(5001..7151)
                     /locus_tag="DRC71_RS11705"
                     /old_locus_tag="DRC71_11790"
     CDS             complement(5001..7151)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728741.1"
                     /locus_tag="DRC71_RS11705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_11790"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_015728741.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.2e-38, bitscore:
                     128.4, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 4.9e-271, bitscore:
                     898.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFSKRFYRAQVDSQDCGAAALAMILEFYGSHYSLDFLRRKLRTT
                     VNGTTAYGLVQVADKLGFETVPIKADISLFEIDDVQYPFIVHVKKNNNHFHYYVVIRCD
                     KKYIYIADPDPVVNLTKMTYREFKEEWTGVTILLKPTSLYKPIKQDKVRLTSFIPLLLK
                     EKRLIIKIIIISILVNMINILGSYFMKSIIDFYIPQKLKTWVSMISMALIASYIIQQIL
                     SYLQKYFLILLVKTYSINILSPYIKHLFRLPITFFETRRAGDILSRLNDANSITDALAS
                     TMLSLFLDVTIIVGISIFLYYQSTILFVLILIIIPIYAVIVFGFMNSFERKRIKSMEAN
                     ATLSSYLMENITGMETIKALTCEKKCYKQIKYNFSNYYEKTFLLNKLNIKQNVLKKVVQ
                     LILNVSILWVGANLVIDNQISIGTLLVFNSLVIYFMNPIENIMNLQLKLQIAGVANNRL
                     NEIFTVKSEFEKNKLDIKLNQNSSKLIFSNISYGYGYEKEILTDINITIEHNSKVAFIG
                     ESGSGKTTLAKMMVSFFEPSKGKITLGGLDITNINKSVLRQQIIYIPQQTYIFNGTILE
                     NLLLGVVKEVTQKDIDSAIKMAELKSFIDSLPLGLDSVLSSDFSALSGGQRQKIALARA
                     LLTNSSILIMDEATSNIDILTERKIINNLMALNKTIIFISHNLNITQKVDKIFVLAKGK
                     IIEEGSHEFLLKAKGYYSNLIN"
     gene            complement(7276..7458)
                     /locus_tag="DRC71_RS11710"
                     /old_locus_tag="DRC71_11795"
     CDS             complement(7276..7458)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="DRC71_RS11710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="DRC71_11795"
                     /product="hypothetical protein"
                     /protein_id="WP_037543780.1"
                     /transl_table=11
                     /translation="MKNIDKFKVISNEVLKDLSGGMSRCKWAQIACINDSMLVRFGTSD
                     PESACDYMKKICKDE"
     gene            complement(7530..7838)
                     /locus_tag="DRC71_RS11715"
                     /old_locus_tag="DRC71_11800"
     CDS             complement(7530..7838)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024424.5"
                     /locus_tag="DRC71_RS11715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_11800"
                     /product="DUF3884 family protein"
                     /protein_id="WP_049770161.1"
                     /transl_table=11
                     /translation="MIKISLKKILCQLSQKKLYEKTFQSIYIVDFSLLDRVPLFKDEFK
                     VIGTWYSYSGKRWICHTELSTEQFKKMITKNIDHKDLKKVKFYLDYLPFSITNEIPF"
     gene            complement(7910..9199)
                     /locus_tag="DRC71_RS11720"
                     /old_locus_tag="DRC71_11805"
     CDS             complement(7910..9199)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.5"
                     /locus_tag="DRC71_RS11720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_11805"
                     /product="glycosyltransferase"
                     /protein_id="WP_051144739.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.1e-18,
                     bitscore: 64.1, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNIYPIFDEDWLEKNKAFLNISCETYNKIKELNKFIESKDWESK
                     RVKNDTELIEKINKLELISEYTTISVVYIVKNERDYIIKSLLSILDLADEIIIVDTGSE
                     DNTLDLIKKMCDKKIKIFTFNWCDDFSKARNFANAKATCDWIMILDADEIVRKNDNLKF
                     YLTYLRIFDDFENTAFNFKVIRDEEVYKTSKLIRNTNTLTYKGRVHETFFSLNNSVSYA
                     NLNVEVLGIRRGSQKKTNYYNKLLLKTIIDFPKEGRWYYLYLRDNIENMNYNQMLKFCK
                     KIIFKRDAISGIEINNNYYSVRIIFLLMFKLLEESNHEGFIYYMELLEKDFNHTQDFIF
                     LKYSYYIKKLNIDATNLLKSFLENYDLLDEGDVIFSKECTHSLLGAYLFWGGYLKESKK
                     VFEELEVNGKDTVCFYNKKFIYDCLSTLSK"
ORIGIN
        1 caatattcat taaaacataa ttcattgtaa aaataacaca atttgcatct aaaaaattta
       61 aatcatctac tatcgacacg tctcactttt ccattcttta ccactcaata agaaatcgtt
      121 tatttgtaaa aaaggagcca ggaattcccc cagctccatc tgatgtttta cgaacgatgc
      181 attttatatt cttgaatcgc ttctaaaaat tcaggacaat agtgcttcag cttataacta
      241 ccaacaccat caatctgaat catttcttgc tttgtgacag gctttctttt cgcaaaagct
      301 tctaacgtat agtctgtaaa aatattcgtc ggtggcatgt ttaatcgttc gctcatttca
      361 cgtctgactt cgacaagctt ttcaaataac gtgcggtcga ccccttctac agtgttaata
      421 tgcactgttt ctttcgactt acgcttgaac ggccttgtaa acacttgcgt atcctcactc
      481 aataactttt ctactgaggc atcacacatg agtatttcgt catgttcgtt gagaaagcct
      541 ttaaagcgga gttcatcgat taaatgatgt aactcgccag tcgtataatc tttcatcaaa
      601 ccatacgtcg aaagattttc atagccttga tgacgaatgt agtccgaaac ctcgccacgc
      661 aaaacttgaa tgaccgtttg atatccttct tgttgacgta aacgtgcgac accgctaatg
      721 atcattttcg cttctttcgt catattgtac gttttatttt tatcaataca attactacat
      781 tggtgacatt cttcaagttt ttcattcggc tcaaaataat ggactaatgt tgcttctaga
      841 catttcgtcg ttttcgtata ttgaatcatt ttcgtcaatt tttcgcccat acgttccttg
      901 taatcatcgt ccgctttcga ggcaccgata aaatactgat gtaaagcaat atctcgatca
      961 ctgtacaata aaatacaatc actatctaat ccgtctcgac ctgcacgtcc tgcttcttga
     1021 taatacgatt ccaaatcacc cggcatattg taatgaatga cataacggac attcgattta
     1081 tcaattccca taccgaatgc gttggtcgca acgactacac gagtacggtc atataaaaag
     1141 tcattctggg cttcatctct ttgtttcgct gtcattcctg catgatagta ggatacaggg
     1201 atatctgcgt cttctaaagc ttcatacaag gcttctactt gtttacgcgt tgaacaataa
     1261 acaatccccg ctttctgact atgtttttga atataatcca atacaaactt ttgacgttga
     1321 tacgtcccat tcacctggaa cttcaaatta cgtcgacgaa tactagtttt gactaagtgt
     1381 tgtcgcgaaa tcattaaccg ttccataata tcttgttgca cttcagtcgt cgctgttgca
     1441 gtcaatgcga caatcgaaaa ctgttgcggc atggtcaata cgcgttgtat gactgcttga
     1501 taactcggcc gaaaatcatg tccccatttt gaaatacagt gtgcttcgtc aaatgcgacg
     1561 agtgaaatat cgacacgtcg caacaacatt tcaaaataag tttgctcaaa acgttccggt
     1621 gcgatgtata aaaatttcaa ctcaccattg accaattgcg tttctatctc tttttgtgct
     1681 tgcgtactta aactactatt caaataagcc gcagatattc ccattgcggt caattggtcc
     1741 acttggtctt tcattaacga aattaaagga ctaatgacaa tggtcgttcc tttaaacatc
     1801 aatcccggta cttgataaca taacgattta cccccacctg tgggtaacac ccctaacgta
     1861 tgatgatgat tcaatacgcg ttcaataatt tctttttgac caggacgaaa agatttgtat
     1921 ccaaaataat gcgacaatgt ttgctccatg ctatccctct attgttctaa tatttcttct
     1981 agttcactcc accgcgtcat gtcggtatca tattgagctt cgagcgtttc tttttcttca
     2041 tttaatgctt tgattttggc ataatctgcg ttcgcttcga tcatttcggc gtcaatttca
     2101 gccagacgcg actctgtttc ctcgatgcgc actaataaca tgtcatattc tcgtttttcg
     2161 ttgtatgata atcctttttt cttttttggt tgtaatgtcg gttgttgttt cacggacgct
     2221 acttgagttg ctgcttgttc caattgtttt tggtatgcta aataatcttc aaactgccct
     2281 aaaatcatgt caatttgacc gtcgtgaata taccaataac aatccacgac tttatctaaa
     2341 aagtaacggt catggctcac agtaatgacg gtaccgccaa atgtttgaat gtagtcttct
     2401 aaaatcgtta acgtttctgt atccaaatca tttgtcggtt cgtctaacaa caacacgttc
     2461 ggctgatgca ctaacaactt caataaatac aaacgctttt gttcgccacc tgagagttta
     2521 tacactttct taccatgcgt cacactgggg aataaaaagc gttccaacaa ttgcgtaatc
     2581 gaaacggtcg tcccatcttt ttgacgcgcc aactcacttt cttcacgcaa gaaatcaatg
     2641 acacggacat cacgattcaa tcgttcatcc gtttgtttaa aatatgccac ttttaccgtt
     2701 tgacctatct ttaatacacc ttcaaaatgc tgatcttcac ctgctaaaat gttcagcatc
     2761 gtcgttttgc cgacaccgtt tgggcccaca ataccgattt gaacaccttt ttgaatcaat
     2821 gtcgacacgt tttcaaataa tgtacggcca tcaatacgtt tcgtcacatt ttcaagttca
     2881 aagacttgtt tgcctaagcg cgaataagcc agattcaatg accctttccc ttgcacttga
     2941 tgcgattgta cttgttcttt caagtcatga aaacgatctt gccttgcgct ttgacaccgg
     3001 cacgcatcca cgctaactct tgtttaaaaa gtgcacgttg tttcgtttgt tgacgcactt
     3061 cgatttcttc ccgctctgct cgcattgcaa tatacgcttc atagttacct ggatatgtat
     3121 ctagcttgcc atttcgcaat tctacaatac gcgtcgccac ttcatttaaa aagtaacggt
     3181 catgcgtgac aaaaacgact gttttcggat aagactttac aaattgaatt aaccaatgaa
     3241 tcgattcgaa atctaaatgg ttcgtcggtt cgtctaataa taataaatct ggtttttcaa
     3301 tcaacgtacg cgctaacact acgcgctttt gttggccacc tgagagttca ctcattcggc
     3361 gtgtcgtatc atggatgcca agtttcgata aaatcgtttt aatttcagca ctataatccc
     3421 aagcactgta agcatccata tcagcttgtg ctgccatcat tgcatcaaac gctttgtcag
     3481 tctgttgctc tgcgtattgt gcagtagctt cttcataacg tttaatgact tgcaactctt
     3541 tggaatctgc actataaacc gcttcataaa ccgttaacgt tggctccaaa ttatgatttt
     3601 gtgccgcata acgaatacga tattgtttcg gatgcgattg aacggcatca aaatcttcat
     3661 ccatgcctgc gatgactttg agcaatgaac ttttccccgt tccattaata ccaactaatc
     3721 cgattcgctc ttgattggaa atggataact gtaaatcatc aaaaatgact ttatccgcat
     3781 aagatttgtt gaggcgttca attttatacg cttccacaac acatacaccc tttttcttat
     3841 agatttactc gcatttatct gttaacctta aatcagttct atatattata cacttttttt
     3901 ggaacaaatg atacaaggag ctatttctat gttggattat atttcaaaca tgccaccctt
     3961 tttacaagca ttacttgcag gtgtcatcac gtggttatta accgcattag gggctgcaac
     4021 cgtatttgta tttaaaagtg tcaacgaaaa agtgttgaat tcgatgcaag gctttgctgc
     4081 tggtattatg atcgccgcaa gtttttggtc attactccaa ccggccattg aaagcagttc
     4141 gggcagtact gtaccgtggc tccctgctgc aattggcttt ttactcggcg gggtattcat
     4201 ccgttcttta gactatgtta ttccacacat gcatcgaaac gcaactgatg aatcacaaca
     4261 tcaagaaggt gttgagacga gtctaaataa aaacacactt ttattacttg cgattacatt
     4321 gcataacatt cctgaagggc tcgcaatcgg ggttgccttt ggtggtattg cgacaggaaa
     4381 tgaacacgca actttgttag gggcactcgg acttgcaatt ggtattggga ttcaaaacat
     4441 tcctgaaggg gctgcattat cattaccgat acatgcttca ggacagtcca aatggaaagc
     4501 cttcaactat ggacaagctt cagcacttgt agaacctgtt tttgctacag tgggcgcagc
     4561 tgctgtgttg gtcgttactc cgatactacc atacgcactc gcttttgctg caggggcgat
     4621 gatttttgtc gtcgttgaag aattaattcc tgattcacaa gccggtaaaa acacagatct
     4681 tgcgacacta agcttgatgg ttggctttac catcatgatg atattagatg ttgctttagg
     4741 gtaaattcaa aaaaccgttc aatgtgatga aacactgagc ggttttttga tgtgtacata
     4801 tatcgataca ttaaaaatac tctttaacaa ctcacaacaa agatttcaca ctgttgacta
     4861 tataaaatat caaccaatat accaaaatat aataattaac ttaagttata attgtaaaat
     4921 tcagaaaata aaaggtgtga aaaaacagta ccttcatttc gttcttcaca ttggttgtgt
     4981 taatacaaaa aataccatta ttaattaatt aagttagaat agtaaccttt tgcttttaat
     5041 aaaaattcat gtgagccctc ttctataatt tttccttttg ctaaaacaaa tattttatct
     5101 actttttgag ttatatttag attatgggag ataaatataa tagttttgtt taacgccatc
     5161 aaattattaa taatttttct ctccgtgagt atatcaatat tactcgttgc ttcatccatt
     5221 atcaatatag aagaatttgt taatagtgct cttgctaaag ctatcttctg ccgctgacct
     5281 cctgacaaag cagaaaaatc agaacttaat acactatcaa gtccaagtgg taagctatca
     5341 ataaaggatt ttagttcagc cattttaata gcactatcta tgtccttttg cgtaacctct
     5401 ttaactaccc ctaataaaag attttcaagt atagttccat taaaaatata agtttgttgt
     5461 ggaatgtata taatctgctg tcgtaaaaca gatttattga tatttgttat gtccaatcca
     5521 cctagtgtta ttttaccctt tgaaggttca aaaaaactaa ccatcatttt cgccaaagtt
     5581 gttttacctg atccagactc tccaataaag gctactttac tattatgttc tattgttata
     5641 ttaatatctg taaggatttc tttttcgtac ccatatccat atgaaatatt tgaaaatata
     5701 agtttacttg agttttggtt caatttgata tccaatttat tcttttcaaa ttctgattta
     5761 actgtgaata tttcattcaa tcgattatta gccacacctg ctatttgtaa tttaagctgt
     5821 aaattcataa tattttctat tggattcata aagtaaatca ctaatgaatt aaaaacaagc
     5881 aaagttccaa ttgagatttg attatctata actaggttag cgcctaccca taaaatcgat
     5941 acatttaata ttaattgaac aacctttttc aaaacgtttt gctttatatt tagtttgttt
     6001 aacaagaatg ttttttcgta ataattcgaa aaattatatt ttatctgctt atagcatttc
     6061 ttttcacatg tcagtgcttt tatcgtctcc atacctgtta tattttccat caaataagaa
     6121 gacaaagtag catttgcctc catagatttt attcttttcc gttcaaaaga gttcataaaa
     6181 ccaaaaacaa ttacggcata aataggaatg ataattaata ttaatacaaa taaaatagta
     6241 ctttgataat acaggaagat agatattcca actattattg taacatctaa aaacaatgat
     6301 agcatggtac tagctaaagc gtcagtaatt gaatttgcat cattaagtct tgatagtatg
     6361 tctcctgctc ttctcgtttc aaaaaaagta ataggtaatc taaacaaatg tttaatgtat
     6421 ggtgaaagta tatttataga atatgtcttt actaataaaa tcaaaaaata tttctgcaaa
     6481 taactaagaa tttgttgtat aatgtaagaa gcaattaaag ccatggaaat catactaacc
     6541 catgttttta acttttgcgg aatataaaaa tcaataattg acttcataaa atatgaacct
     6601 aaaatattta tcatattgac taaaatggaa ataattatta tttttataat caaccttttt
     6661 tcttttaata ataaaggtat aaaagaagtt aaacgaactt tatcttgctt tataggttta
     6721 tataaagatg taggttttaa taagattgta actccagtcc actcttcttt aaattcccta
     6781 taagtcatct ttgttaaatt aacaacaggg tcagggtcag ctatatagat atacttttta
     6841 tcacatctaa ttacaacata atagtgaaaa tgattgttat tttttttaac atgaacgata
     6901 aaaggatact gtacatcatc aatttcaaac aaagatatat ctgctttaat aggaactgtc
     6961 tcaaaaccta acttatctgc tacctgaacc aaaccatatg ctgtagtgcc atttactgtt
     7021 gttcttaact ttcttctcag aaaatccaaa gaatagtgag aaccgtaaaa ttctaatatc
     7081 atagcgagcg ctgccgcgcc acaatcttga gaatctactt gtgcacgata aaatcgtttt
     7141 gaaaatttca ttcgacactt ctactccttc ccaccacttt ttagaacaac atttaaatat
     7201 tcaaaaagcg ggcggtaatt cccacccact taatatcatg tcaaaattca atcctattat
     7261 taaaagcttt aatggttatt catctttaca tatttttttc atataatcac atgcactctc
     7321 tggatcagat gtaccgaatc taaccaacat actgtcgttt atacatgcaa tctgagccca
     7381 tttacaacgg gacatcccac cacttaagtc tttcagtacc tcatttgata tcactttaaa
     7441 tttatcaata tttttcatat ctattcctcc taaacaaaac aatttaataa taatattaaa
     7501 aattaattca ttaaatatat aatttatttt caaaacggaa tctcgttagt tatagaaaat
     7561 gggagataat ccaaataaaa tttgactttt tttaaatctt tatggtctat attttttgtt
     7621 atcattttct tgaattgctc ggttgataat tccgtatggc atatccaacg cttaccacta
     7681 tagctatacc aagttcctat tactttaaat tcatctttaa atagaggaac cctatctaaa
     7741 agactaaaat caactatata aatactttga aatgtttttt cataaagttt tttttgagat
     7801 aattgacata aaattttctt taaagatatt ttaatcatag atttctcact ccttcacact
     7861 acctgttacc ttaatgcggt agattcctga gctataataa tatcaatatt tatttgctta
     7921 aagtcgatag acaatcataa ataaattttt tattataaaa acaaactgta tctttaccat
     7981 ttacttctaa ttcttcaaat acttttttac tttcctttaa atatcctccc caaaataaat
     8041 aggctcctaa taacgaatga gtgcattctt tcgaaaaaat tacatctccc tcatctaaca
     8101 aatcataatt ctctaagaaa cttttcaata aatttgtagc atctatatta agtttcttta
     8161 tatagtatga atatttcaaa aaaataaagt cttgagtatg attaaaatct ttctctaaca
     8221 actccatata gtaaataaaa ccttcatgat tggattcttc taataactta aacatcaaaa
     8281 ggaaaataat tctaacagag taataattgt tgtttatttc gattccagat atcgcatctc
     8341 ttttaaatat aattttttta caaaatttta acatttgatt ataattcata ttttctatat
     8401 tatctctcaa atataaataa taccaacgac cttcttttgg gaaatctata attgttttaa
     8461 gaagcaattt attataataa tttgttttct tttgacttcc tcttctaatt cctaaaactt
     8521 ctacatttaa attagcataa gatacagaat tatttaaaga aaagaatgtt tcgtgtactc
     8581 tacctttgta cgtcaaagta ttagtatttc ttatcaactt acttgtttta tatacttctt
     8641 catctctaat tactttaaaa ttaaaggccg tattttcaaa gtcgtcgaaa attcttaaat
     8701 acgttaagta aaactttaaa ttatcatttt ttctaacaat ttcgtcagca tctaaaatca
     8761 taatccagtc acaagttgct ttagcatttg caaagtttct tgcttttgaa aaatcatcac
     8821 accaattaaa tgtaaatatt tttatttttt tatcacacat ttttttaatt aaatctaaag
     8881 tgttgtcttc agaaccagtg tcaacaataa tgatttcatc ggctaaatct aaaatactca
     8941 aaaggctttt tataatataa tctctttcgt ttttaacaat atacactaca gaaattgtag
     9001 tatattcact tataagctct aatttattta ttttttcaat caattctgta tcgtttttaa
     9061 ctcttttaga ctcccaatct tttgattcta taaatttatt taattctttt attttgttgt
     9121 atgtttcaca agaaatgtta agaaatgcct tatttttttc taaccaatct tcatcaaata
     9181 tagggtatat gtttttcaaa gttaatcaca ccgcctctaa aaaataataa aacaaaatat
     9241 ttttaaaagt tttgcaagac tgggaggcga attattatga taaatacgta attctactat
     9301 cacatttaca aattgctatg ctttaattat aaataattgt attactttgt aaatatagtg
     9361 tcaatattta tttatattcg gaagtataat ttctcacata tactttaatt acagaagtac
     9421 atgcagtgta gatgacggtt ataaaatgta ggaaaatggc gaaatgaaaa tgctgcattt
     9481 catatataaa cgccaagata aatatggaca agtttattct ttttatgaaa ggaaatgttt
     9541 taatctaaaa tactttccaa cgatagaggc gattgagacg ggttctttgt caacgtcggt
     9601 tggtgagaca ataacggcac tgagacatta tggtttccta atctctgctt ctatttatag
     9661 actgatatcg attaataatg attgatcctt cgaaaataag gggtgaatgt gtataaaaaa
     9721 tgatgccatt gttttctgga tttcataatc ttgcactcag tttcgctgta ttgatatagg
     9781 attacccaga tggctgagac gcctgagggg gaacgtgcat actgttgaca gtcgcctctt
     9841 tactttaatg gtgggaggcg tttatcgcag tagcgggctg acttctcagt gttttcactt
     9901 ttgagaagtc taatcagcca tgcaaggcag tacaacgaaa tcttgttgca tatgaggttt
     9961 ctgtactgct cctaaaatcc aatttggctt caatcaagcc gaattgagtt gaagggccgg
    10021 ctctaggaaa gcgaaaccat gatgacaatt aaaaccacaa atcgtaatgc ggacaagttt
    10081 aataaaaata ttgcaacaat agcatcaaaa atttttaaga cccattccgt tttttatatg
    10141 cacttacaag tatcttagaa atgattaaga ttccagaaat ttctataact ataagacaca
    10201 catttgtttt attattaatc ctttcaatcg ggccatttgt taaatgagga tgatgcatag
    10261 tatttgaggt tcagggcaaa tatttgatga atgtttgcaa cgattaggta aaatgtcgat
    10321 gatatcatgg gttacactat cacagtaaat aaagctcatt ggtccttcga tatctttcgt
    10381 tgatttgaat tcgtcaaaag ctaagtgctc aggtaaagca tcatgtgcgt atgtctttac
    10441 aaattactca cctcttttaa atggcaatga acagttgaag gtgatacaca tagactttta
    10501 gcgacatctt tttcagatgt gaatttagtt agcttccgcg tcatcaagcg tttgacctta
    10561 tttgagactt cggggttgaa tatagggtgt tttagttata aaggtttgtt gacaagcttt
    10621 aaagtaaaaa cgatgcttct tcaaatttaa ataaacaggt ctttcaaaat ttaaaaacgg
    10681 gcagtaaatc tctcaccaac cgttaaaaat acacaaccaa atatatacaa cctaaaaaac
    10741 agggaaatgc actaatttgt acacttccct atcataacat tttatttttg atttgccttt
    10801 ggacctgtcg gatatttata atctgctggg ttcaatttgt tgaaatcagg atttttatag
    10861 aatcggaata agtctccatt caagacttta tcactcattt ctaagtcatg ttgtacctgc
    10921 tttttgtcct tatcaagatc tgctggtggt gtttctaacg gttgattgtc tctgttactg
    10981 taggctttac cgttgacata tttataattt ctcgtcacga aatcaccatt acggaatggt
    11041 acaatttctt tatggtcctt ggataacata tctgtaccaa acatgagata atttttcgta
    11101 tcaataccta acaagtgaag taatgttggc atcacatcaa cttgacctgc atacgtgtcg
    11161 tccactttag gctcaacccc tggtgctttt aaccagaaac ccgttgcatt caagtcatta
    11221 aacttcgctg gtgtaatctc ttcacctaat aattgtgaca tcgctttgtt atgattttct
    11281 gagataccat aatggtcacc gtaaatcata atgactgaat catcgtacag accacgctct
    11341 ttcaagtcat taatgaactg ttctaacgac tcatctaagt aacgtgctgt ttgaatgtaa
    11401 ccatcgaccg ttgagttacc tgtgtcacct tttgcaatcg ttgcgtcttc atcacttaat
    11461 gtaaaaggat aatggttcgt caacgtaatc aaatgtgaat agaacggttg tttttcttta
    11521 gataagtaat ccgccgcctc tttgaaaaac tctttatctt ttaatccaag gttttctaag
    11581 ttttctggag acatgtcaaa atatgttgca tcataaaact tatcgatacc aaaatgtctg
    11641 tatacttggt cacggttcca aaatgtttta tagtcaccgt gcatgacatt tgttgtatag
    11701 ccttgttgtt gatgcataat agctggtaaa gattgaaaag tattgtcacc ttttaatgaa
    11761 aaggccgaac cttgaggtaa accatacata ctgttgtcca ttgtgaactc agcgtctgac
    11821 gttttacctt gtccagtttg gtgatagaaa tgtgggtaat atctaaagtc ctcattacct
    11881 gacgacaatt tatttaaaaa cggcgtcact tcctcaccat gaattttttt attaattagg
    11941 aatgtttgga agctttctaa atggatttta atgacatttt tacctttagc ttttccatag
    12001 tattccatat tcggtgtagc ttgtttttgt tttgtataat tcaatacttc tgttaaatca
    12061 tcttcagaag cgagtgcctt ttgttgattg ttctgtactg ttttgacacc atcatatact
    12121 gtaaagttgt aaggtcctaa gtatttcaca a
//
