LOCUS       NZ_CP031605            10291 bp    DNA     circular CON 21-DEC-2024
DEFINITION  Staphylococcus pseudintermedius strain VB16 chromosome, complete
            genome.
ACCESSION   NZ_CP031605
VERSION     NZ_CP031605.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales;
            Staphylococcaceae; Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 2791609)
  AUTHORS   Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2018) Microbiology, Veterinary Science,
            Chulalongkorn University, Henry-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-FEB-2018
            Assembly Method                   :: Canu v. 1.3
            Assembly Name                     :: VB16
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40.0x
            Sequencing Technology             :: Oxford Nanopore Technologies
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007833695.1-RS_2024_12_21
            Annotation Date                   :: 12/21/2024 01:51:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,737
            CDSs (total)                      :: 2,655
            Genes (coding)                    :: 2,571
            CDSs (with protein)               :: 2,571
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 37 of 84
            Pseudo Genes (incomplete)         :: 50 of 84
            Pseudo Genes (internal stop)      :: 12 of 84
            Pseudo Genes (multiple problems)  :: 13 of 84
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:07:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 124753
            Orig. end                         :: 135044
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP031605.1.
            Bacteria and source DNA available from Nuvee prapasarakul,
            Henri-Dunant Rd., Pathumwan, Bangkok 10330, Thailand.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10291
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10291
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10291
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5291](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5291)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            198..1589
                     /locus_tag="DRC71_RS00545"
                     /old_locus_tag="DRC71_00560"
     CDS             198..1589
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1212:
                     sodium:dicarboxylate symporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015728531.1"
                     /locus_tag="DRC71_RS00545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_00560"
                     /product="L-cystine transporter"
                     /protein_id="WP_015728531.1"
                     /transl_table=11
                     /translation="MSIFLVIINLIIFLLALLSLWVMAKKHVKFPKRVFFALGLGIVLG
                     LLLHLVYGVEGTVTSQTTDWVGLIGNGYIALLQMIVIPLIFVSIVAAFTKIEIGEKFAK
                     VGSFIFMFLIGTVTIAAMIGIIYALAFNLDASSIDLGQAENARSAEIADKAKGLTATTL
                     PTQILELLPSNPFLDFTGARATSTIAVVIFAAFVGFAFLRVARKQPENGQTLKRGIDAI
                     YALVMAIVTFVLRLTPYGILAIMINTIATSDFAAIWTLGKFVIASYAALITMYIIHLII
                     LAVIGVNPVQYVKKTAEVILFAFTSRSSAGTLPLNVQAQTNRLGVPQAIANFSGSFGLS
                     IGQNGCAGIYPAMLAIMVAPVAGVEVNLQFIVTVIIVVVLSSFGVAGVGGGATFASILV
                     LSALNLPVGLAGVLISVEPLIDMGRTALNVNDSILAGTGTAKLTKQLDEDVFNSNHMDE
                     LTAQH"
     gene            complement(2022..2678)
                     /locus_tag="DRC71_RS00550"
                     /old_locus_tag="DRC71_00565"
     CDS             complement(2022..2678)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571171.1"
                     /locus_tag="DRC71_RS00550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_00565"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_037541928.1"
                     /transl_table=11
                     /translation="MIELKNITIQKGKRTIFDNVNLKFEPGKSYALIGHSGSGKSTLLN
                     TIAGFETLKKGEVQLDQKRLKADFRFYRDILGYVFQNYGLVDSMTINQNLDMALAFKKG
                     SRSGKKQLKTHSLQKVGLDIEGKRKVATLSGGEQQRVALARLILKQPRLILADEPTGSL
                     DEANGQKVMELLFDMVDDTRTLIIATHDLTLAQQCDEIIQVDTLKCHSSELETVQ"
     gene            complement(2675..3004)
                     /locus_tag="DRC71_RS00555"
                     /old_locus_tag="DRC71_00570"
     CDS             complement(2675..3004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463765.1"
                     /locus_tag="DRC71_RS00555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_00570"
                     /product="YxeA family protein"
                     /protein_id="WP_037541929.1"
                     /transl_table=11
                     /translation="MKKFILAAFAIIVVAVGLFLAFARIPYVDYFNPFLKKETSYAVVP
                     LGTQTYVDIQAYDESGEPLKYRLNFGGYDANFDHVKVLHKGTYVFHIDYIKDLSKWPKK
                     VSVEE"
     gene            complement(3008..4975)
                     /locus_tag="DRC71_RS00560"
                     /old_locus_tag="DRC71_00575"
     CDS             complement(3008..4975)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614907.1"
                     /locus_tag="DRC71_RS00560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_00575"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_096533766.1"
                     /transl_table=11
                     /translation="MKWLKHLLDVFTLTLISLFLMVLLYEEDSEILTGSQVAIQVEGWD
                     YQYSKAEVFDRFERVAKDLDIAIFKVITDHKKGQVDKAIYTFNKKANHHTITPMNRSYS
                     YQQLTLDDLMKRDVRGDYFILDSVANPHQIKAALESAGLKVAVVPIKRWMIYIDVLINR
                     GVLLPFVTLLIIYILYHLYDRSKNFKTYATMRLHGYRFYNIFFLNIKKIVARWFLLAMG
                     VSLFSIGLLKWLGLDGQLGYFISHLILADIWFWMILFISSLLSYILLVSMNVPLMIKGL
                     KPYRLLHLINHVSKLSMLVLLTLLILPNFNQLKKLEKIQETETWWGKLDDYYMIELKPI
                     RRSINEERNFAKRFHQMIVYSEQHEQALLMRQNVLAEPSETNFVPGNGNVLFVNQNFVD
                     FFKSQLQDLPRLEDREGQVELYLPPQAKTKAAAIRDDFQEWVDFQLPNSATNTKVKVTQ
                     VDNPYQIYAFDVRTGLSTAYLKSPAILMLNAKDLTDDFYYATLSQGTFIFKNYENIIRN
                     IDRFNLKDDVQGVTNYKDNVIKKYREAQTQWIVYSFSSGLAISVLFIVTLLDVLHYFEQ
                     HRQWLLMRKMFGFRRWQNYRNYIILNGLFTALIGAFLFEKTQNSNVIWLFAIVLLFQFF
                     VQWVYIHYLEKQFNVLIREV"
     gene            complement(5001..5291)
                     /locus_tag="DRC71_RS00565"
                     /old_locus_tag="DRC71_00580"
     CDS             complement(5001..5291)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /locus_tag="DRC71_RS00565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_00580"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_037541931.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 6.2e-22,
                     bitscore: 75.3, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKKFLSTLTAGLLVVFIGATSLVSAESVHAEGGIWNHGVGSKYV
                     WSYYSHNGKYHTSTAIGKYRSDSGATKPGEEAQASAEKRWWWRNEAYYSVL"
     misc_feature    5441..5454
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.72"
                     /tool="antismash"
     gene            complement(6041..6796)
                     /gene="nfsA"
                     /locus_tag="DRC71_RS00570"
                     /old_locus_tag="DRC71_00585"
     CDS             complement(6041..6796)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="nfsA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011276836.1"
                     /locus_tag="DRC71_RS00570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_00585"
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="WP_096533765.1"
                     /sec_met_domain="PF00881 (E-value: 2.8e-20, bitscore: 70.4,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEYVYDLAKRHHSVRKFKQTPIERATIEKLIEAGQMASTSSFLQ
                     TTSFIGVESIEKKEALREVSGQPYVVENGYLLVYVIDYHRHQLVNEKSKADMAASFESA
                     EGLLVGTVDAALAAQNIALTAEDMGYGIVYLGSLRNDVQRVREILNLPEHVFPLFGMAI
                     GEPADDENGSPKPRLPLSHVFHVDTYNQDDAELSEQIEAYDQTVSQYYQERTQGKRDET
                     WSEQIAGFMSSKQRLEMNDWLQMSGFNKK"
     misc_feature    complement(6974..6988)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.15"
                     /tool="antismash"
     misc_feature    7259..7272
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 24.83"
                     /tool="antismash"
     gene            7349..7918
                     /gene="ahpC"
                     /locus_tag="DRC71_RS00575"
                     /old_locus_tag="DRC71_00590"
     CDS             7349..7918
                     /EC_number="1.11.1.26"
                     /GO_function="GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ahpC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /locus_tag="DRC71_RS00575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_00590"
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="WP_014614909.1"
                     /transl_table=11
                     /translation="MSLIGKQIEEFSAQAYNAKTDEFIEVTHEDLKGNWNVVVFYPADF
                     SFVCPTELEDVQNHYEKLQELGTNVFSVSTDTHFVHKAWHDHSDAISKLQYTMIGDPSQ
                     AITRQFDVLDETSGLAQRGTFIIDPDGVVQAAEINADGIGRDASTLVHKIKAAQYVRQN
                     PGEVCPAKWEEGSETLTPGLDLVGKI"
     gene            7933..9456
                     /gene="ahpF"
                     /locus_tag="DRC71_RS00580"
                     /old_locus_tag="DRC71_00595"
     CDS             7933..9456
                     /GO_function="GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_function="GO:0051287 - NAD binding [Evidence IEA]"
                     /GO_process="GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /gene="ahpF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451490.1"
                     /locus_tag="DRC71_RS00580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="DRC71_00595"
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="WP_015728526.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.8e-42, bitscore:
                     142.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLNQELKQQLSQLLDLMEGDVVLKVSTGDDDHSQKMNDLVNEVSD
                     MSSRITVEKAELKRTPSFSINRPNEDTGITFAGVPLGHEFNSFVLALLQVSGRAPKEEQ
                     SVIDQIKSIDEPLHFETFISLTCQKCPDVVQALNLMSVINPNITHTMIDGAVFKKEAED
                     IMAVPAIFLNGEQFGNGRMTVTDILSELGQGPDASEFENKETFDVLVVGGGPASASSAI
                     YAARKGLKTGIVADRIGGQVNDTADIENLIGVKKTTGPSLATSLEEHIKDYNVDIMTGV
                     RAEGLEKTDDIVHLTLDNGAVLKTRSLIIATGARWKKIGVPGEDTFANKGVAYCPHCDG
                     PLFEGKDVAVIGGGNSGVEAAIDLAGICKSVTVLEYGESLKADSVLQERLNSLPNTTVI
                     THAATQEIKGDDRVSGIAYTCNETNQEKHVDLNGVFVQIGLSPNTEWLGDTVQRNRMGE
                     IEVDRLGNTNIPGVFAAGDCTDQRYKQIIISMGSGATAALSAFDYLIRN"
ORIGIN
        1 cattatcgta acgggatttt actgtcaccc attgctggtc aactgatggc gcaactcgtt
       61 gatgaagata gcgaagcccg acatcagctt gctccattta gacctaaaat aaaacaaata
      121 taaaaaatat atcttgattt tatgtttcct tactattatc ctattataga agtcggaatt
      181 aaaaaggaag cgtatttatg tctatttttt tagtgattat taatttaatt atttttcttc
      241 tcgccttatt aagcctgtgg gtgatggcga aaaaacatgt caaattccct aaacgcgtgt
      301 tctttgcact tggtttaggt atcgtattag gcctcctact ccaccttgtt tatggtgttg
      361 aaggcacagt cacatctcaa acaacggatt gggtcggctt aattgggaat gggtatattg
      421 cattgctaca aatgattgtc attccactta tttttgtttc tatcgtcgcg gcatttacga
      481 aaattgaaat tggagaaaag tttgctaaag tcggcagctt catcttcatg tttttgattg
      541 gtaccgtaac aattgcagcg atgatcggta ttatctatgc actcgcattc aatttagatg
      601 cttcaagtat tgacttaggg caggcagaaa acgctcgtag cgccgaaatt gctgataaag
      661 caaaagggct gactgcgaca acattaccaa cacaaatttt agaattgtta ccgtcaaatc
      721 catttttaga tttcactggt gcacgtgcaa cttctactat tgcagtcgtg atctttgccg
      781 catttgtagg ctttgcattt ttacgtgtcg cacgtaaaca acctgaaaat ggtcaaactt
      841 taaaacgcgg tattgatgcc atttacgcac ttgtcatggc tatcgtaaca tttgttttac
      901 gcctaacgcc ttatgggatt ttagcgatta tgattaacac gattgcgacg agtgattttg
      961 ctgcgatatg gactctcggt aagttcgtga ttgcgtccta tgcagcactc attacaatgt
     1021 acatcattca cttaatcatt ttggccgtta ttggtgtcaa tccagtacag tatgtgaaga
     1081 aaacagccga agtgatttta ttcgcattca cttcacgctc aagtgccggt acattgccac
     1141 tcaacgtaca agcgcaaacg aatcgtctag gtgtgcctca agctatcgca aacttctcgg
     1201 gctcatttgg actatctatc ggacaaaatg gctgtgcggg aatctaccct gccatgcttg
     1261 cgattatggt cgcacctgtc gcgggtgttg aagtgaatct acaatttatc gtgacagtca
     1321 tcatcgttgt cgtgttaagt tcattcggtg tagcaggtgt agggggcggt gcaacattcg
     1381 cttcaatcct ggtcttatct gcattgaact tgccggttgg ccttgcgggt gtcctcattt
     1441 cagtcgaacc attgattgat atgggtcgta cagcacttaa cgtgaatgac tctatcttag
     1501 cgggtacagg cactgctaaa ctgacaaaac aactcgatga agatgttttc aattccaatc
     1561 atatggacga attaacagca caacattaag taacacaata gttacaaatt attagttcta
     1621 ggattgtctt gatggattcg ctttcccagg ggacttgcct caactagcca acgcttaatc
     1681 atactgtgac ataagtgaag cgttggcgga tttttggctg cgtctgatcc cttgggagtc
     1741 tcatccatca cgacaatctt actctacaaa gtcatagaaa gagggcaact ttttacatta
     1801 atgcgatgta acttgctttt gatttcatcg ttaattactt gctttattgt aaattagatt
     1861 aaagtgtaca tttcgtacat actgaagctt aattttcata gagcgtctat aaaggcaatc
     1921 atatgtatat aaacataatc aaagagacag aaggcaagtt ggctgtgata cgccatttct
     1981 ttccagctaa cattagtgcc cctatctctt ttaacttatg tttattgtac ggtttctaat
     2041 tcgctactgt gacattttag tgtatcaact tgaataatct cgtcacattg ttgcgcgagc
     2101 gttaaatcat gcgtcgcgat aatcagtgtg cgcgtatcat cgaccatatc aaataacagt
     2161 tccatcactt tttgtccatt tgcttcatct aaagaacccg tgggctcatc cgctaaaatg
     2221 agtcttggtt gtttcaatat cagacgagca agggcgacac gctgttgctc acccccactt
     2281 aatgtcgcaa ctttacgctt gccttcaatg tcaaggccca ctttttgcaa actatgtgtc
     2341 tttaattgtt tctttcctga tctagaaccc tttttaaaag ccagtgccat atcgagattt
     2401 tggttaatcg tcattgaatc aacaagacca taattttgaa atacatagcc gaggatatcg
     2461 cgataaaaac gaaaatcagc tttcaatctt ttttgatcga gttgcacttc accttttttt
     2521 aacgtttcaa aacctgcaat ggtattcagc aaagtcgatt tccccgagcc actatgacca
     2581 ataagcgcat aagacttccc cggctcgaat tttagattca cgttgtcaaa aatcgtacgt
     2641 ttcccttttt gtatcgttat atttttcaat tcaatcattc ttcaacactc acctttttag
     2701 gccatttcga aagatcttta atataatcga tatgaaaaac gtaagtccct ttgtgaagca
     2761 ctttcacatg atcaaaattg gcgtcatatc ccccgaaatt taaacgatat ttaaggggtt
     2821 cacctgattc gtcatacgct tgaatatcca cgtatgtttg tgttcccaat ggcactactg
     2881 cataagaggt ctctttttta agaaagggat taaaataatc gacataggga atacgtgcaa
     2941 aagctaaaaa taaccccact gccactacga taatagcaaa cgcagccaaa ataaatttct
     3001 tcactaccta cacctcccta attaatacgt taaattgttt ttctaaatag tggatgtaaa
     3061 cccattgaac gaagaattga aacagtaaaa caatggcgaa taaccatatc acattactat
     3121 tttgtgtctt ttcaaataag aacgcgccaa taagagcagt aaataaacca tttaatatga
     3181 tatagttgcg atagttttgc caacgtctga agccaaacat tttccgcatg agcagccatt
     3241 gacggtgctg ttcaaagtaa tgtaacacat ctaataacgt gacgatgaat aatactgata
     3301 tcgcgagccc actagaaaag ctatatacaa tccattgtgt ttgtgcttct cgatattttt
     3361 taatcacatt atctttatag ttcgttacac cttgcacgtc atccttcaaa ttaaaacggt
     3421 cgatattacg aatgatattt tcataatttt taaagatgaa agtaccttgt gataaagtgg
     3481 cgtaatagaa atcatctgtt aaatctttgg cgtttaacat caaaattgcg ggtgacttca
     3541 aataagctgt agacaaacct gtccgtacat caaaggcgta aatttggtat ggattgtcca
     3601 cctgtgtcac tttaactttt gtattagttg ctgaatttgg aagttgaaag tctacccatt
     3661 cttgaaaatc atcacgtata gctgcggctt ttgtttttgc ttgaggcggt aaatacaact
     3721 ctacctgccc ctctcgatct tccaaacgcg gtaaatcttg taattgtgac ttaaaaaagt
     3781 ctacaaagtt ttgattaaca aacagcacgt tcccattccc tggtacaaaa ttcgtttcgc
     3841 ttggctcagc tagcacattt tgtcgcatca gtaatgcttg ttcatgttgc tcgctatata
     3901 cgatcatttg atgaaagcgc tttgcgaaat ttctttcttc atttatactg cgccgtattg
     3961 gtttcaattc aatcatatag taatcgtcta atttgcccca ccatgtttct gtctcctgaa
     4021 ttttttctaa cttttttagc tgattaaagt taggcaatat aagcagcgta agtaaaacga
     4081 gcatacttaa cttagataca tgattgataa ggtgtaataa tcgatacggt ttaaggcctt
     4141 taatcattaa tggtacattc atacttacaa gcaaaatata agatagcaaa gaacttatga
     4201 ataaaatcat ccaaaaccat atgtccgcta agataagatg gctaataaaa tacccgagtt
     4261 gtccatccaa gcctaaccat ttcagcagtc caattgagaa gagactgacc cccatcgcta
     4321 aaaggaacca tctcgccact attttcttaa tattcagaaa aaatatattg taaaaacggt
     4381 aaccgtgtaa acgcatcgtc gcatacgttt taaaattttt acttcgatca tacaaatggt
     4441 aaagtatata tatgatgagc aatgtgacaa aggggagtag gacgcctcga ttaatgagta
     4501 catcgatata aatcatccat cgctttatcg gaacaacagc tactttaagc ccagcacttt
     4561 ctaaagctgc tttgatttga tgaggatttg cgacactatc caatatgaaa taatcccccc
     4621 gtacatcccg cttcatcaag tcatcaaggg ttaactgttg atagctatat gacctattca
     4681 ttggcgtgat tgtgtgatga ttcgcttttt tattgaaagt atagatggcc ttatcaactt
     4741 gccccttttt atgatccgta attactttaa aaatcgcaat atctaagtct ttcgcaaccc
     4801 tttcaaaacg atcaaaaact tctgctttcg aatattgata atcccaacct tctacttgaa
     4861 tggccacttg gctcccggta agtatttcac tatcttcttc atacaacaat accattaaaa
     4921 atagacttat tagcgtgagt gtaaacacat ctaataagtg tttcaaccat ttcatgactc
     4981 atcccctaaa actctgataa ttataataca ctataatatg cttcattacg ccaccaccat
     5041 ctcttctccg ctgacgcctg agcttcttct ccaggtttag tagctccact atctgatcta
     5101 tattttccta ttgctgtgga agtgtgatac tttccattat gactataata tgaccatacg
     5161 tatttacttc ccacgccatg gttccatatt ccaccttctg catgaacact ttctgcactc
     5221 actaaacttg ttgcaccaat aaaaacgact aataatcctg ctgtaagtgt acttaaaaat
     5281 ttctttttca taactgcttc ctcctttttg atagaattac acctatatta tatttttaaa
     5341 caactatatt tgcaacataa ttatattact tttaataact agttttttat atattacaat
     5401 taatttataa taagtaacta aagtgagtaa aaaaatgctc attttataca aaaaattctt
     5461 cctagtcaat tatttagatt aaagaggtac attttttaag taaaaccact caaaggttaa
     5521 agggctggat tattttcata tgatactttt tttacttgcg caaacctgtt ccttttactt
     5581 ttttgggggg ataagattgc caaaaagggt gagactcccg agggatcaga cgcagccgaa
     5641 aatccgccaa cgcttcaata atgtaacagt tcaatcaagc gttggctagt tgaggcaagt
     5701 cctgaacttg aacccctcca gtttacactt aaatatcaaa aactgatgtc tagtttagct
     5761 tatcctgcta agctagacat tttcaatgaa acttgttcgc tttcttcaaa tgttggcgtg
     5821 attgccgtat ggacgagact cttgagggat cagctgaacc ggaaaatcca ccaactcttc
     5881 tgcaagtgta acagtataat caaagcgttg gttagttgaa ggttcagccc ctaagaacgc
     5941 gagtccaaga cggcaatcat atgcaaacag catgttcgaa taatgaaaag gcaagttcag
     6001 caaacttagc tctgtcaaaa taatattagc cctttcaccc ctatttcttg ttaaatcctg
     6061 acatttgcaa ccaatcattc atttctaatc gttgcttgct actcataaag ccagcaattt
     6121 gttcagacca agtttcatca cgtttacctt gtgtacgttc ttgataatat tgagaaaccg
     6181 tttggtcata cgcttcaatt tgttcagaca attcagcatc gtcttgattg tacgtatcta
     6241 catgaaagac atgcgataat ggaaggcgcg gtttaggtga accattttcg tcatctgccg
     6301 gctctccaat ggccataccg aataatggga agacgtgttc aggtaaattt aaaatttcgc
     6361 gtacccgttg cacatcgtta cgtaatgaac cgagatatac aatgccgtaa cccatatctt
     6421 cagccgttaa tgcaatattt tgcgccgcta atgccgcgtc aactgtaccg actaataacc
     6481 cttcagccga ttcaaagctc gctgccatgt ctgctttcga tttttcatta accaactggt
     6541 ggcgatggta atcaatcaca taaacgagca agtacccatt ttcaacgaca taaggctgac
     6601 ctgagacttc tctaagtgcc tctttctttt caatagattc taccccaata aatgatgtcg
     6661 tttgtaaaaa actcgatgtc gatgccattt gtcctgcttc tattaatttt tcaattgttg
     6721 ctcgttcgat aggtgtttgt ttgaactttc tcactgaatg gtgacgtttc gccaaatcat
     6781 acacatattc tgacataaaa acactcctaa catccttttc tctatcgtaa catttgccct
     6841 actaaaacgc atgattcgtg catgcttcct agctatcata ctcgtacttt attaaattag
     6901 cgaaaatcac atctgttatg acaacatcaa caaagtgctt tctatattta ccctttaatt
     6961 gagaatgaaa gcatataaat gatattcaaa tcttttatca attaacttat ttccattttt
     7021 atttccaata catcgtctgc tttattaact ttactacaat gagaaaataa tgcgtatcaa
     7081 tcaccaaatg tgaccgaatc gtgatcaaaa agtggctttt ttactgtatt tatgctttat
     7141 tgcacctata acatcaaaac cgctattaat gatgaaagcg tttgacttca aagtggataa
     7201 aaatctttta tatttatttg acgtaatgat tgttccatgt cataatacta cttgagatta
     7261 gaattattat taataaggaa ttatcacaaa gtaaaacttg gttctaatcc aaataataat
     7321 gattcatata aataggagga ttttgattat gtcattaatc ggtaaacaaa ttgaggaatt
     7381 ttcagcacaa gcatacaatg caaaaacaga tgaatttatt gaagttacgc acgaagattt
     7441 aaaaggtaac tggaatgttg tcgtattcta ccctgcagat ttctcattcg tatgtccaac
     7501 tgaattagaa gatgtacaaa atcattacga aaaattacaa gagttaggta caaacgtatt
     7561 ctctgtttct acagatactc actttgtaca taaagcatgg cacgatcatt cagatgccat
     7621 cagcaaatta caatatacaa tgattggtga tccttctcaa gcgattacac gtcaattcga
     7681 tgtattagat gaaacgtcag gtcttgcaca acgtggtact ttcattattg acccagacgg
     7741 tgttgttcaa gcagcagaaa tcaatgcaga cggtatcggt cgtgacgcaa gcacactcgt
     7801 acacaaaatt aaagcagcac aatatgttcg tcaaaatcca ggtgaagttt gcccagcgaa
     7861 atgggaagaa ggtagcgaaa ctttaactcc aggattagat ttagtaggta aaatttaagg
     7921 aggctatact gaatgttaaa tcaagaatta aagcaacaac tttcacaact tcttgatttg
     7981 atggaaggtg acgttgttct gaaagtgagc actggcgatg acgatcattc tcaaaaaatg
     8041 aacgacctcg ttaatgaagt ttcagacatg tcatctcgaa ttacagtaga aaaagctgaa
     8101 ttaaagcgta cacctagctt tagtatcaac agaccaaacg aggacactgg cattacattt
     8161 gctggtgtcc ctcttggtca tgagttcaat tcttttgtcc tagcactctt acaagtgagt
     8221 ggtcgtgcac caaaagaaga gcaatctgta attgatcaaa tcaagtctat tgatgaaccc
     8281 cttcactttg aaacattcat tagcttaaca tgtcaaaaat gtcctgatgt cgttcaagca
     8341 ttaaacttaa tgagtgtcat taatccgaat ataacacata caatgattga tggtgcagtt
     8401 ttcaaaaaag aagcagaaga cattatggcg gtacctgcaa ttttcttaaa tggtgaacaa
     8461 ttcggtaacg gccgcatgac agtgacagac attcttagtg agttaggtca aggtcctgac
     8521 gcttctgaat ttgaaaataa agaaacattt gacgtcctcg tagtcggcgg cggtcctgca
     8581 agtgcaagct ctgccatcta tgctgcgcgt aaaggtttaa aaacaggtat cgtagcagac
     8641 agaattggtg gtcaagttaa tgacacagct gatattgaaa acttaattgg cgtgaagaaa
     8701 acgacaggtc catcacttgc gacaagtctt gaagaacaca ttaaagacta caatgtggat
     8761 attatgaccg gcgtacgtgc agaaggatta gaaaaaacag atgatatcgt tcatttgact
     8821 ttagataatg gcgcagtgct taaaacgcgc tcactcatta tcgcaacagg tgcacgttgg
     8881 aagaaaatcg gtgtaccggg tgaagacaca ttcgcaaaca aaggtgttgc atactgcccg
     8941 cactgtgacg gacctctatt tgaaggtaaa gacgtcgctg ttatcggtgg cggtaactct
     9001 ggtgttgaag ccgcgattga cttagcaggt atttgtaaaa gtgtgacagt ccttgaatat
     9061 ggtgagtcat taaaagcaga ctctgtatta caagagcgtt taaattcatt accgaataca
     9121 acagtcatca cacatgcagc aacacaagaa atcaaaggtg acgaccgcgt gagtggtatt
     9181 gcttacactt gtaacgaaac aaatcaagaa aaacatgttg atttaaatgg tgtcttcgtt
     9241 caaatcggtc tttctcctaa tactgaatgg ttaggcgata ctgttcaacg taaccgcatg
     9301 ggtgaaattg aagtcgaccg tttaggaaac acgaacattc ctggcgtctt tgcggcaggc
     9361 gactgtacag accaacgcta taaacaaatc attatttcta tggggtcagg cgcaacagca
     9421 gcattatctg cattcgacta cttgattcgt aactaaatag gattcatatc attaatcgga
     9481 ctggtgagaa agggttatct tttcatcagt ccgattttct attttatgca cgtaaaggca
     9541 acacagcttt ttcatccatc gaagtaagca agtgagagcc ttttgagtgc aatcataaaa
     9601 ggacaaacac cttaaaatgg taaagcgtgg ttctacattc tgaaatcaac gttatcctac
     9661 aaaaatgaaa tcaaccagtt ccaaatttta gtaaaaatgc tgacaatggc atcccacaat
     9721 ttttgcaaga aattacgcgt ttcctcatta tctaaatcct tcagcttctt cagtttgtcg
     9781 ccggcttggc tttgaatcat ttcttttaac tcagtcgcct gttttttaaa cgcatccgga
     9841 tcttggttga ctacattgga ttccgcgacg ttcaccatga tattttgaat gaccgcaatt
     9901 tcgtgatcac ttaaaatctg atacagtccg cgctctgtta atgtttggtt gacaatttga
     9961 ttgattgttg tactgttcag ttgttgatgt gacgcttttg cttcagcgat ttgagctttc
    10021 atttctgcaa ctgcttcatt caacgcttca tcagaatagc catctttatt ttgatgattt
    10081 tcactaattc gtgctaaatg attcatttct tggttcgcat tttgaatatc ttgcgcattt
    10141 aaacgatgac cttgtgtggc ataggcttta taaatgcctg tcaatgcacc ttcgccagtc
    10201 acttgatcga cagaagcaat tttaatcgtc gcatcttgaa tacctgatgt aatcgctgca
    10261 ttcatatact gttctcgtgt aatacgtgta a
//
