LOCUS       NZ_VLJL01000050        10387 bp    DNA     linear   CON 03-JUL-2024
DEFINITION  Bacillus subtilis J27 BacJ27_scaffold_8, whole genome shotgun
            sequence.
ACCESSION   NZ_VLJL01000050
VERSION     NZ_VLJL01000050.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis J27
  ORGANISM  Bacillus subtilis J27
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 142389)
  AUTHORS   Gladden,J.
  TITLE     Genome sequencing of lignin-degrading bacterial isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 142389)
  AUTHORS   Gladden,J., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.0.19
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 510.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007829735.1-RS_2024_07_03
            Annotation Date                   :: 07/03/2024 03:39:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,202
            CDSs (total)                      :: 4,129
            Genes (coding)                    :: 3,934
            CDSs (with protein)               :: 3,934
            Genes (RNA)                       :: 73
            rRNAs                             :: 5, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 5, 1 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 128 of 195
            Pseudo Genes (incomplete)         :: 68 of 195
            Pseudo Genes (internal stop)      :: 26 of 195
            Pseudo Genes (multiple problems)  :: 24 of 195
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:40:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 99167
            Orig. end                         :: 109554
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VLJL01000050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10387
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10387
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10387
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5387](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5387
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            336..1883
                     /gene="pruA"
                     /locus_tag="L604_RS20945"
                     /old_locus_tag="L604_000900001030"
     CDS             336..1883
                     /EC_number="1.2.1.88"
                     /GO_function="GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA]"
                     /GO_process="GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pruA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388203.2"
                     /locus_tag="L604_RS20945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000900001030"
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="WP_144531492.1"
                     /sec_met_domain="Aldedh (E-value: 2.2e-155, bitscore:
                     510.7, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPYKHEPFTNFQDQNNVEAFKKALATVSEYLGKDYPLVINGER
                     VETEAKIVSINPADKEEVVGRVSKASQEHAEQAIQAAAKAFEEWRYTSPEERAAVLFRA
                     AAKVRRRKHEFSALLVKEAGKPWNEADADTAEAIDFMEYYARQMIELAKGKPVNSREGE
                     KNQYVYTPTGVTVVIPPWNFLFAIMAGTTVAPIVTGNTVVLKPASATPVIAAKFVEVLE
                     ESGLPKGVVNFVPGSGAEVGDYLVDHLKTSLITFTGSREVGTRIFERAAKVQPGQQHLK
                     RVIAEMGGKDTVVVDEDADIELAAQSIFTSAFGFAGQKCSAGSRAVVHEKVYDQVLERV
                     IEITESKVTAKPDSADVYMGPVIDQGSYDKIMSYIEIGKQEGRLVSGGTGDDSKGYFIK
                     PTIFADLDPKARLMQEEIFGPVVAFCKVSDFDEALEVANNTEYGLTGAVITNNRKHIER
                     AKQEFHVGNLYFNRNCTGAIVGYHPFGGFKMSGTDSKAGGPDYLALHMQAKTISEMF"
     gene            2007..3428
                     /gene="putP"
                     /locus_tag="L604_RS20950"
                     /old_locus_tag="L604_000900001040"
     CDS             2007..3428
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA]"
                     /GO_function="GO:0031402 - sodium ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /GO_process="GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /function="16.1: Circulate"
                     /gene="putP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388204.2"
                     /locus_tag="L604_RS20950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000900001040"
                     /product="sodium/proline symporter PutP"
                     /protein_id="WP_010886399.1"
                     /transl_table=11
                     /translation="MLLIGYFAYKRTSNLTDYMLGGRSLGPAVTALSAGAADMSGWLLM
                     GLPGAMFSTGLSGAWIVIGLCLGAWANWLYVAPRLRTYTEKAGNSITIPGFLENRFGDQ
                     TKLLRLFSGIVILVFFTFYVSSGMVSGGVLFNSILGMDYHTGLWIVTGVVVAYTLFGGF
                     LAVSWTDFVQGIIMFAALILVPIVTFFHTGGAGDTVAEIRSVDPDMFNIFKGTSVLGII
                     SLFAWGLGYFGQPHIIVRFMAITSVKEIKRARRIGMGWMILSAVGAVLTGLGGIAYYHQ
                     RGMTLKDPETIFIQLGNILFHPIITGFLISAILAAIMSTISSQLLVTSSSLVEDLYKSM
                     FRRSASDKELVFLGRLAVLAVSIVALVLAWEKNNTILGLVSYAWAGFGASFGPVVLLSL
                     FWKRMTKWGALAGMIVGAATVIIWANAGLSDFLYEMIPGFAASLLSVFFVSILTQAPSQ
                     AVTDQFNDYQDTMSQ"
     gene            3581..4810
                     /gene="putR"
                     /gene_synonym="ycgP"
                     /locus_tag="L604_RS20955"
                     /old_locus_tag="L604_000900001050"
     CDS             3581..4810
                     /codon_start=1
                     /gene="putR"
                     /gene_synonym="ycgP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328047.1"
                     /locus_tag="L604_RS20955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000900001050"
                     /product="proline utilization transcriptional regulator
                     PutR"
                     /protein_id="WP_042977244.1"
                     /transl_table=11
                     /translation="MEELLERVFSFSDVDKLIDFISYELQKPVILESADFFLLAYNSYY
                     INHFDSANQQTIFSKKCPVQIFERFLKDGIIEKLKTEPEPFRVNKIESIGLNQRVVVSA
                     KHKGEVMGYIWIQELDQNLTDEELDFLYETSFHVGKIIYKTNKLKQEKEEKAEDLIKRA
                     IYQQFTSEKELRREAERINTVLPSMFSVVILHAANGDGEAVEDLKENIRSYLNLRDKVS
                     HVLTIESNIVIVVASFSQKSSVSSAASEFINKLLTHFHFQKIPTPIYIGIGNEYNHLLK
                     LGKSYTEALEVIKAAEITGNQENIPYEYAKLGIYRYLESIEQKNEFLEYENKDLALLKA
                     KDEESSTELLKTLEIYLLNNCKTKPAAEQLFIHQNTLNYRIKQITEMTSIDLSDFRTRC
                     QLYLDLMLMK"
     misc_feature    complement(4950..4964)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.21"
                     /tool="antismash"
     misc_feature    4951..4965
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 20.52"
                     /tool="antismash"
     gene            5001..5387
                     /locus_tag="L604_RS20960"
                     /old_locus_tag="L604_000900001060"
     CDS             5001..5387
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328048.1"
                     /locus_tag="L604_RS20960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000900001060"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_042977243.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.8e-08,
                     bitscore: 28.0, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKKGLMSAALIAALALSTGQAASAAGAATSGEVDLTNTPLKNSE
                     VTPFANENVGGGNWDYGTAGYFDWGFKKKVWSNYYHKDKAHASSCALGTRTNSSGKTKA
                     GTTSYSDLSGGATDAKTKANWKTY"
     gene            5467..7674
                     /locus_tag="L604_RS20965"
                     /old_locus_tag="L604_000900001070"
     CDS             5467..7674
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328049.1"
                     /locus_tag="L604_RS20965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000900001070"
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WP_042977242.1"
                     /transl_table=11
                     /translation="MRKIVILFLVISSLFSFLITFRETANKQIEEIEQLDSSVGKPFTI
                     PDDPLLNDPKEMIELIMSSSKKYKTNIFRPATNFSTNDKVEISKYVLLTQQTDYFNGIE
                     LSQGKFLSKLDTQSENFYLSTHKSKDKNQIGQIEKFGITSEITIKPLKNSFDYLPVNGT
                     YYTEIKGKYSFEDFLKGFVNEINEKFDSTFEINDFLQTREVNDSYAEISTSSLQQYNYL
                     ILIVTLFLLIYYIVNESKTIGVLKLHGYSNLRIWFQIVGKLLILTFCFSLLLSIICAIF
                     LPGSTFSFIMNTIMKQIINYLIIGSLSILTYFYIRKVKVNLAIKNKKDTNSIFIFNSIL
                     KIVCSVLIIVIGSSTLNQYSEVKKKQSHLEDWKVSKDYGVFNPLYIGHDIEDVNNGKPQ
                     LTNNIDNIYPILNKMGALLIDTREYEEEFIRLNSSYKGIRSIVVNDNYLKQFPVYNSEK
                     QPISINEREKRLILLVPEKYKQHREKIIKFFKEDRKESAVAYDEEFLGRHVPKYLKEPK
                     IHIIWTASGQKIFSFNPEVFKAEDNLIIDPIIQVVTENNSLVGDRNTILGNGSRDPLKI
                     KLNNRDSKKTMNILVPYLKKYHLDDNLKYLVSLNQYALNEIKELHSEISSLLALAAVLA
                     VALVLLIVQNSVIYFNKKQQKFVVYRLFGIGFFRTFKDYMTLFFILWLIQFISCIVIKR
                     GITLNLLICIVFLLILELVTSIIALIIIERRNKIKVMKGGF"
     misc_feature    5477..5493
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.44"
                     /tool="antismash"
     gene            7679..8323
                     /locus_tag="L604_RS20970"
                     /old_locus_tag="L604_000900001080"
     CDS             7679..8323
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328050.1"
                     /locus_tag="L604_RS20970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000900001080"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_042977240.1"
                     /transl_table=11
                     /translation="MSYVMELQNIRKAYGKHVVLENVNIVIEKGEMVAITGSSGSGKTT
                     ILNIMGMLEKPDGGIVKILGDVAPRIGSGRANELLRSKISYLFQNYALIDNASISANLD
                     VPLTYKKLKREEKEKLKIAVLEKVGLQKVSLTQKVHELSGGEQQRLAIARIILKPCDII
                     LADEPTGSLDVKNRNEIITLLKELNQMNKTIIIVTHDPYVSNQCSRVIELN"
     gene            complement(8412..9269)
                     /gene="ycgQ"
                     /locus_tag="L604_RS20975"
                     /old_locus_tag="L604_000900001090"
     CDS             complement(8412..9269)
                     /codon_start=1
                     /gene="ycgQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388206.1"
                     /locus_tag="L604_RS20975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000900001090"
                     /product="TIGR03943 family protein"
                     /protein_id="WP_042977239.1"
                     /transl_table=11
                     /translation="MFRLLVLMGFTFFFYHLHASGNLTKYINMKYAYLSFIAIFLLAIL
                     TAVQAYLFIKSPEKSGHHHDHDCGCGHDHEHDHEQNKPFYQRYLIYVVFLFPLVSGIFF
                     PIATLDSSIVKTKGFSFKAMESGDHYSQTQYLRPDASLYYAQDSYDKQMKQLFNKYSSK
                     KEISLTDDDFLKGMETIYNYPGEFLGRTIDFHGFAYKGNAINKNQLFVLRFGIIHCIAD
                     SGVYGMLVEFPKDMDIKDDEWIHIKGTLASEYYQPFKSTLPIVKVTDWNTIKKPDDPYV
                     YRGY"
     gene            complement(9274..10155)
                     /gene="ycgR"
                     /locus_tag="L604_RS20980"
                     /old_locus_tag="L604_000900001100"
     CDS             complement(9274..10155)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /gene="ycgR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388207.1"
                     /locus_tag="L604_RS20980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000900001100"
                     /product="permease"
                     /protein_id="WP_144531511.1"
                     /transl_table=11
                     /translation="MTAQSSFLQLNSIFISILIEAIPFILIGVILSGIIQMFVSEEMIA
                     RIMPKNRFLPVLFGALAGVLFPACECGIIPITRRLLLKGVPLHAGVAFMLTAPIINPIV
                     LFSTYIAFGNRWSVVFYRGGLALAVSLIIGVILSYQFKDTQLLKPDEPGHHHHHGTLLQ
                     KLGGTLRHAIDEFFSVGKYLIIGAFIAAAMQTYVKTSTLLAIGQNDVSSSLVMMGLAFV
                     LSLCSEVDAFIASSFSSTFSLGSLIAFLVFGAMVDIKNLLMMLAAFKKRFVFLLITYIV
                     VIVLAGSLLVKG"
ORIGIN
        1 cgatgtcgat gaaaattaca aaaaaatcat ccgaaagcag ctcttaaacg gtcactatac
       61 agcgattgcc acacatgacg acaaaatgat cgactttaca aagcagcttg ccaaggaaca
      121 tggcattgcc aatgacaagt ttgaatttca gatgctgtac ggcatgcggt cgcaaaccca
      181 gctcagcctc gtaaaagaag gttataacat gagagtctac ctgccatacg gcgaggattg
      241 gtacggctac tttatgagac gccttgcaga acgtccgtca aacattgcat ttgctttcaa
      301 aggaatgaca aagaagtaaa aaaggagaga ttatcatgac aacaccttac aaacacgagc
      361 cattcacaaa tttccaagat caaaacaacg tggaagcgtt taaaaaagcg cttgcgacag
      421 taagcgaata tttaggaaaa gactatccgc ttgtcattaa cggcgagaga gtggaaacgg
      481 aagcgaaaat cgtttcaatc aacccggctg ataaagaaga agtcgtcggc cgagtgtcaa
      541 aagcgtctca agagcacgct gagcaagcga ttcaagcggc tgcaaaagca tttgaagagt
      601 ggagatacac gtctcctgaa gagagagcgg ctgtcctgtt ccgcgctgct gccaaagtcc
      661 gcagaagaaa acatgaattc tcagctttgc ttgtgaaaga agcaggaaag ccttggaacg
      721 aggcggatgc cgatacggct gaagcgattg acttcatgga gtattatgca cgccaaatga
      781 tcgaactggc aaaaggcaaa ccggtcaaca gccgtgaagg cgagaaaaac caatatgtat
      841 acacgccgac tggagtgaca gtcgttatcc cgccttggaa cttcttgttt gcgatcatgg
      901 caggcacaac agtggcgccg atcgttactg gaaacacagt ggttctgaaa cctgcgagtg
      961 ctacacctgt tattgcagca aaatttgttg aggtgcttga agagtccgga ttgccaaaag
     1021 gcgtagtcaa ctttgttccg ggaagcggag cggaagtagg cgactatctt gttgaccatc
     1081 tgaaaacaag ccttatcaca tttacgggat caagagaagt tggtacgaga attttcgaac
     1141 gcgcggcgaa ggttcagccg ggccagcagc atttaaagcg tgtcatcgct gaaatgggcg
     1201 gtaaagatac ggttgttgtt gatgaggatg cggacattga attagcggct caatcgatct
     1261 ttacttcagc attcggcttt gcgggacaaa aatgctctgc aggttcacgt gcagttgttc
     1321 atgaaaaagt gtatgatcaa gtattagagc gtgtcattga aattacggaa tcaaaagtaa
     1381 cagctaaacc tgacagtgca gatgtttata tgggacctgt cattgaccaa ggttcttatg
     1441 ataaaattat gagctatatt gagatcggaa aacaggaagg gcgtttagta agcggcggta
     1501 ctggtgatga ttcgaaagga tacttcatca aaccgacgat cttcgctgac cttgatccga
     1561 aagcaagact catgcaggaa gaaattttcg gacctgtcgt tgcattttgt aaagtgtcag
     1621 actttgatga agctttagaa gtggcaaaca atactgaata tggtttgaca ggcgcggtta
     1681 tcacaaacaa ccgcaagcac atcgagcgtg cgaaacagga attccatgtc ggaaacctat
     1741 acttcaaccg caactgtaca ggtgctatcg tcggctacca tccgtttggc ggcttcaaaa
     1801 tgtcgggaac ggattcaaaa gcaggcgggc cggattactt ggctctgcat atgcaagcaa
     1861 aaacaatcag tgaaatgttc taagcgggac taaatgggca tcctccctgc gggggtgtcc
     1921 atttcatcca taaactataa aaaagaggag gaagtgccat agaaaacaca cagctgatta
     1981 tttcgatttg tatttatatg gcgggaatgc tgctaatcgg ctactttgct tacaagcgta
     2041 cgtcgaatct gacggattac atgctgggag gacgctcttt gggtccggca gtaactgctc
     2101 tcagtgccgg cgctgccgat atgagcggct ggctgctaat ggggcttccg ggcgccatgt
     2161 tttctactgg tctgagcggc gcttggatcg tcatcggact ttgccttgga gcgtgggcga
     2221 actggcttta cgtcgcaccg cggctgagaa cctatactga aaaagcgggg aattccatta
     2281 caattcccgg attcctggaa aatcgcttcg gagatcaaac aaagcttctc agactgtttt
     2341 caggaattgt cattttagtc tttttcacgt tttatgtatc gtccggaatg gtgtccggcg
     2401 gcgttttatt caacagcatc ttggggatgg attatcacac cggcctgtgg attgtgacag
     2461 gtgttgttgt ggcatatacc ctgtttggcg gctttttggc agtcagctgg acagattttg
     2521 ttcaggggat cattatgttt gccgcactca ttcttgtgcc gatcgttacg tttttccaca
     2581 cgggaggagc aggtgataca gtcgctgaaa tccgctctgt agatcctgat atgtttaaca
     2641 tttttaaagg aacgagcgtc cttggcatta tttctttgtt tgcctggggg ctgggatact
     2701 ttggccagcc gcatattatt gtgcgtttta tggcgatcac gtctgtcaaa gagatcaaaa
     2761 gagcgcgcag aatcgggatg ggctggatga ttttgtctgc agtaggcgca gtgctgaccg
     2821 gtttgggcgg aatcgcttat taccatcagc gcggcatgac actgaaggac ccagagacga
     2881 tttttattca gttagggaat atcttgttcc atccgattat cacgggtttt ctgatttcag
     2941 cgattttggc cgcgattatg agtacaattt cttcccagct gcttgtgaca tcaagctctc
     3001 tggtagaaga tttgtataag tcgatgttca gacgctcagc ttcagataaa gaactggtgt
     3061 ttttaggccg actggctgtt ctggctgttt caattgtcgc gttggttctg gcttgggaga
     3121 aaaataacac catccttgga ttggtcagct acgcgtgggc gggcttcggt gcatcattcg
     3181 ggccggttgt actgctcagt ctgttttgga aacgaatgac caaatggggg gcactcgccg
     3241 gcatgattgt gggagcagcg actgtaatca tttgggcgaa tgccggtctt tcggactttc
     3301 tgtatgaaat gatacctggt tttgctgcga gtctattatc tgtctttttt gtcagcatat
     3361 tgacgcaggc tccgtcacaa gctgtcacag accagttcaa cgactaccaa gatacaatgt
     3421 cgcaataaag atcgaaagga ggaggaaggg gctgtccctt tttcctcttt tctcctttta
     3481 agcggccatt tttttctttt ggaaacaaac gtattttttt ctatacaaaa agaccccgta
     3541 tggatatgat taaagtaaaa aaagtatagg agaaaaaggt atggaagagc ttttagagag
     3601 agttttttca ttttcagatg ttgataagct aattgatttt attagctatg aactgcaaaa
     3661 gccagtgata ctggaaagcg cggatttctt tttgttagcc tataattctt actatattaa
     3721 ccattttgat tctgccaacc agcagactat tttttcgaaa aagtgtccgg tccagatttt
     3781 tgagaggttt ctgaaggacg gaattattga aaaattgaaa acagagcctg agccttttcg
     3841 cgtcaataaa attgaaagca tcgggttaaa ccagagggtg gtcgtgagtg caaagcacaa
     3901 aggggaagtc atgggctaca tctggattca ggagctggac caaaatctga cggatgaaga
     3961 gctcgacttt ttgtacgaga cctccttcca tgtcgggaaa atcatttata aaacgaataa
     4021 attgaaacag gaaaaggaag aaaaagcaga ggatctcatc aaacgggcga tctatcagca
     4081 atttacctct gagaaggaac tcagacggga agctgaaagg atcaacaccg tgctgccttc
     4141 catgttttca gttgtgatcc tgcacgccgc aaatggggat ggggaagcgg ttgaggattt
     4201 aaaggaaaat atcaggtcgt acctgaattt acgggataag gtcagtcatg tcttaacgat
     4261 cgaatcaaac attgtcatcg tcgtagcgag tttttcccaa aaaagctccg tctcctcagc
     4321 agcttctgaa tttattaaca agctgttaac acattttcac tttcaaaaaa tacctacccc
     4381 tatttatatc ggtatcggaa acgaatataa tcacctatta aaacttggca agagctacac
     4441 agaagcactt gaagtcatca aagccgcaga aatcaccggc aatcaggaaa acattccata
     4501 tgaatatgcc aaactcggca tttaccgtta tttagaaagc atcgaacaga aaaatgaatt
     4561 tttagaatac gagaataaag atttagcttt attaaaagcg aaagacgaag aaagcagcac
     4621 tgagctgctc aaaaccttgg aaatctatct cctcaacaac tgcaaaacaa aaccagcggc
     4681 agaacagctg tttattcacc aaaatacatt gaattaccgc atcaaacaaa ttactgagat
     4741 gacgtcgatc gatttaagcg atttcagaac gcggtgccag ctctatctcg atttgatgct
     4801 gatgaaataa ancagcctgt ttagagagtt tttctgcgag tttgttaata gaatatatct
     4861 agatcacttt cttttatgat ccatttatag gaaaaatatt tatttactat actgttaata
     4921 tatggtataa tcaggttgtg gttgaacgga tgagcgcgct gtcattcaat ctacatttaa
     4981 aaagaaggag tggatcagtt atggtgaaaa aaggtctcat gagtgcagct ttaatagcag
     5041 cattagcatt atcaactgga caagcagcat cagcagcagg tgcagcaaca agtggcgagg
     5101 ttgatttaac taatacgcct ctgaaaaact ctgaagtaac cccatttgcg aatgaaaatg
     5161 ttggcggagg aaactgggat tacggtacag ctggttattt tgattgggga tttaaaaaga
     5221 aagtttggtc aaattattat cacaaagata aggcccatgc ttcatcatgt gctctaggga
     5281 caagaactaa ttcaagcggt aaaactaaag caggaacaac ctcatactcc gatttgtcag
     5341 gcggcgcaac agatgctaag acaaaggcca attggaaaac ttactaatat aaaattttga
     5401 taaggatatc atcggaaact agagttagtt ttcgatgatt ctccttttta taaaggagtt
     5461 ttttttgtga gaaaaattgt gattttattt ctagtgattt cttcactgtt ctcctttttg
     5521 attaccttta gggaaaccgc aaataaacaa attgaagaaa ttgaacagct agatagttct
     5581 gtcggaaaac cttttactat tcctgatgat cccttactaa atgaccctaa agaaatgatc
     5641 gaactaatta tgagtagttc taaaaaatat aaaactaata tttttcggcc ggctactaat
     5701 ttctctacaa acgataaagt ggaaatttct aaatatgtat tgttaacgca acaaactgac
     5761 tattttaatg gcattgaact ctcacaaggg aaatttttaa gtaagttgga tactcaatct
     5821 gaaaattttt acttaagtac gcataaatca aaggataaaa atcagatagg acaaatagaa
     5881 aaattcggta tcacttctga aataacaatt aaaccactaa aaaactcatt tgattatctt
     5941 cctgtaaacg gtacatatta tactgaaatt aaaggtaagt attcttttga agatttttta
     6001 aagggatttg ttaatgagat aaatgaaaaa tttgattcga cgtttgaaat aaatgacttt
     6061 ttacaaactc gagaagttaa tgatagttat gcggaaatct ctacctcgtc attacaacag
     6121 tacaactatt tgattcttat tgtaacattg tttcttctta tttattatat cgtgaatgag
     6181 tcaaaaacaa taggtgtttt aaaattacat ggatattcta atttaagaat ttggtttcaa
     6241 atagtaggga aattattaat attaacgttt tgtttttctt tgttattatc aatcatatgc
     6301 gcaattttct tacctggttc caccttttct tttattatga ataccatcat gaaacaaatt
     6361 attaattatt tgattatagg ctcattatca atacttactt atttttatat aagaaaggta
     6421 aaagttaatc tagcaataaa aaataagaaa gatacaaata gcatctttat ttttaattct
     6481 attttaaaaa tagtttgttc ggtcttaata attgtgatag gtagttcaac tttaaatcaa
     6541 tatagtgaag tgaaaaaaaa acaatctcat ttagaagatt ggaaggttag taaagattat
     6601 ggggttttca atcctttgta tataggtcat gatattgagg atgttaataa cgggaagcca
     6661 caacttacaa ataatattga taatatttac cccattttga acaaaatggg tgctctctta
     6721 atagatacaa gagaatatga agaggaattt attcgattaa actctagcta taagggaatc
     6781 aggtctattg ttgtaaatga taattattta aaacaatttc ctgtatacaa ctcagaaaaa
     6841 caaccaatat caattaatga gagagaaaaa agattaatat tacttgttcc agagaaatat
     6901 aaacaacata gagaaaaaat aattaaattt tttaaagaag atagaaaaga atctgcagta
     6961 gcttatgatg aagagttttt agggagacat gtaccaaagt atttaaaaga acctaaaata
     7021 catattattt ggactgctag tggtcagaaa atatttagtt ttaacccaga agtttttaaa
     7081 gctgaggata atctaattat agatcctatt atccaagttg ttacagagaa taatagcttg
     7141 gtaggtgaca gaaatacaat tttaggaaat ggaagtcgag atccgcttaa aataaaatta
     7201 aataatagag attcaaaaaa gacaatgaat atattagtgc cttatttaaa aaaatatcat
     7261 cttgatgata atttgaagta cttggtctct ttaaatcaat atgcattgaa tgaaataaaa
     7321 gagttacata gcgaaattag ctcactgtta gctttagctg ctgtattagc tgtcgctttg
     7381 gttcttctta tcgtgcaaaa ttctgtaata tacttcaata agaagcaaca aaagtttgta
     7441 gtctatagat tgtttggtat tggttttttt agaacattta aagattatat gaccttgttt
     7501 tttatactat ggcttattca atttatatct tgtattgtta tcaaaagagg aataacttta
     7561 aacctattaa tttgtattgt atttctttta attttagaat tggttacttc gataattgca
     7621 ttaattatta tagaacgaag aaacaagatt aaagttatga aaggaggttt ctgattaatt
     7681 gagttatgtt atggaattgc aaaatataag aaaagcttac gggaaacatg ttgtattgga
     7741 aaacgtcaac attgttatag aaaaagggga aatggttgca attacaggta gcagtggttc
     7801 gggaaaaaca acaatactta atatcatggg tatgctcgaa aagccagatg gtgggattgt
     7861 taaaatactt ggggatgttg ctccgagaat tggttctgga agagccaatg aattgttgcg
     7921 ttctaaaata tcatatttgt ttcaaaatta cgccttgata gataatgcta gtatcagtgc
     7981 gaatttagat gtcccattaa cttataaaaa gttaaagaga gaagaaaagg aaaaattgaa
     8041 gattgcggtt cttgaaaaag tgggtttgca aaaagtgtcc ttaacacaaa aagtccatga
     8101 gctttctggg ggcgaacaac aaagattagc aatagcaaga atcatattaa aaccatgtga
     8161 tattattctt gcagatgaac caacaggctc tttagatgta aagaatcgca atgaaattat
     8221 caccctttta aaagaattaa atcagatgaa taaaacaata ataatagtaa ctcatgatcc
     8281 ttatgtctca aatcaatgca gtcgagtgat cgaacttaac tgacaaaagt aactaaatta
     8341 atatgtatag ggttaaagtg ctaaaaaata tataagtagg tttgaggaac aattttgttc
     8401 ctcaaacctg tttaatagcc tctgtaaaca tacggatcat caggtttttt aatggtattc
     8461 cagtcagtta ctttgacgat agggagagtt gacttgaatg gctggtaata ttctgaggcc
     8521 agagtgccct taatgtgaat ccattcatca tccttgatgt ccatgtcctt cggaaattcc
     8581 acaagcatcc cgtacacgcc ggaatcagcg atacagtgaa tgatgccaaa ccttaatacg
     8641 aacagctgat ttttgttaat tgcgtttcct ttgtaagcaa atccatggaa atctatcgtc
     8701 cgtccgagaa attcacccgg atagttatag atggtttcca ttccttttag gaaatcgtca
     8761 tcggtcagag aaatctcttt tttgctggaa tacttattaa acagctgttt catttgtttg
     8821 tcatagctgt cttgcgcgta ataaaggctg gcatcaggcc gcaaatactg tgtttgggag
     8881 tagtgatctc cgctctccat cgccttaaac gagaaccctt ttgttttgac tatggatgag
     8941 tcaagcgttg caatcgggaa gaagatgcct gacaccaaag ggaaaagaaa aacaacataa
     9001 atcaaataac gctgataaaa tggcttgttt tgttcatggt catgctcgtg atcatggccg
     9061 cacccgcaat catggtcatg gtgatgtccg cttttttcag gcgacttgat aaatagatat
     9121 gcttggacgg cggtcagaat ggccagcagg aagatcgcaa taaaggaaag ataggcatat
     9181 ttcatattga tatattttgt tagatttcct gaggcatgca gatgataaaa gaaaaacgta
     9241 aatcccatca gcaccaatag acgaaacacc caatcacccc tttaccagta gtgatcccgc
     9301 taaaacaatc acgacaatgt atgtaatcag caggaaaaca aatcgttttt tgaaggccgc
     9361 aagcatcatc aatagattct taatatccac cattgcgcca aagacgagaa aggcgattaa
     9421 cgatcctaat gaaaatgtgc tgctgaagga agacgcgatg aacgcatcaa cttctgaaca
     9481 aagcgacagg acaaacgcca gacccatcat cacgagggaa gaggatacat cattctgccc
     9541 gatcgcaagc agagtggatg ttttgacata cgtctgcata gcggctgcga taaaggcgcc
     9601 gatgattaaa tattttccca cggagaaaaa ttcatcaatg gcatgccgca aggtcccgcc
     9661 cagtttttgg agcaaggtgc cgtgatgatg atggtgcccg ggttcatcag gctttaaaag
     9721 ctgggtatcc ttaaattgat aggataagat gaccccgatg atcagcgaaa cggcgagagc
     9781 cagtccgcct ctgtaaaaga ccacgctcca tctattcccg aacgcaatat aagtagaaaa
     9841 taaaacgata ggattaatga taggcgcggt cagcatgaaa gcaacgcccg catgcagagg
     9901 aactcccttt aacagaagcc gcctcgtaat cgggatgatg ccgcattcgc aggcgggaaa
     9961 taaaacgccg gccagcgcac cgaataaaac ggggagaaaa cggtttttcg gcatgattct
    10021 tgcaatcatt tcttcagaaa caaacatttg aatgattccg gaaagtatga cgccaatcag
    10081 aataaacgga atggcttcta ttaaaatgct gataaaaata gaattcagct gaagaaaaga
    10141 tgattgtgct gtcacaataa aacctccgct catgttaagc tgcctttctt atgataccag
    10201 aaaaaaaggc actgcttgtt tgacggagga ggggaaagga tcgtttcact ttggttagga
    10261 ttccaaaaaa acgagtagga tactgtctgt ttctgcagct tcttttccga gccaaatcag
    10321 gtgtccccat gagtgaagca aacagagaac agcgccacga atgtgctctt ttgcatgatg
    10381 tgcatgt
//
