LOCUS       NZ_VLJL01000048        10225 bp    DNA     linear   CON 03-JUL-2024
DEFINITION  Bacillus subtilis J27 BacJ27_scaffold_6, whole genome shotgun
            sequence.
ACCESSION   NZ_VLJL01000048
VERSION     NZ_VLJL01000048.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis J27
  ORGANISM  Bacillus subtilis J27
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 172527)
  AUTHORS   Gladden,J.
  TITLE     Genome sequencing of lignin-degrading bacterial isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 172527)
  AUTHORS   Gladden,J., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.0.19
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 510.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007829735.1-RS_2024_07_03
            Annotation Date                   :: 07/03/2024 03:39:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,202
            CDSs (total)                      :: 4,129
            Genes (coding)                    :: 3,934
            CDSs (with protein)               :: 3,934
            Genes (RNA)                       :: 73
            rRNAs                             :: 5, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 5, 1 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 128 of 195
            Pseudo Genes (incomplete)         :: 68 of 195
            Pseudo Genes (internal stop)      :: 26 of 195
            Pseudo Genes (multiple problems)  :: 24 of 195
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:40:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 128945
            Orig. end                         :: 139170
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VLJL01000048.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10225
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10225
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10225
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5225](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5225
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    1147..1164
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.18"
                     /tool="antismash"
     gene            complement(1355..2305)
                     /locus_tag="L604_RS21610"
                     /old_locus_tag="L604_000700001390"
     CDS             complement(1355..2305)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="L604_RS21610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000700001390"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_353734205.1"
                     /transl_table=11
                     /translation="MKGTLTIGQLITYNALLTYFLNPLQNIIGLQSKLQSASVASKRLG
                     EILKLEPELKEKEAQITSTSSITGPLSFNKINFRYGTRRLILKDITLHIQKGEKVAFVG
                     ESGSGKSTLTKLLMKFYTAESGEVLINGYHINDIHTNALRESISYIPQESHFIQGSILE
                     NLLLGNSGSFTFEEIIEVCKQTKVHDFINDLPMRYDTLIEENGSNLSGGQKQRLAIARA
                     LLRKPNILIMDESTSNLDTTTEQGISKMIYDQTVGITTIIIAHRLSTIMNCDKIFVMEN
                     GEIIEYGNHNDLLKKKGKYYELWENQMPSRGMVTI"
     gene            complement(<2294..3537)
                     /locus_tag="L604_RS21615"
                     /old_locus_tag="L604_000700001400"
                     /pseudo=""
     CDS             complement(<2294..3537)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /locus_tag="L604_RS21615"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="L604_000700001400"
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /pseudo=""
                     /sec_met_domain="Peptidase_C39 (E-value: 5.3e-45, bitscore:
                     146.1, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFFNRYHCVRQLDQKDCGAACLSTIAKHYGSKLSLAYIRDIAGTD
                     TQGTSALGMVKAAEQLGFSAKAVRAEHTAFDQSITLPAIAHIIIDQQLLHYVVIHKISK
                     DKIIIADPGKGLVTYQKDDFFKIWTGILILLAPTETYKKQTETSNISADIFRLILQQKS
                     LIIHTFLASFIITIFGILGTFYFQTIIDQILPNGLSNTLHILSIGLLIMYLFKVFLIGF
                     RQYLMILLGQKLSISIMFGYFQHVLTLPMKFFSNRKVGEIISDLLMLIK"
     misc_feature    complement(3717..3732)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 22.73"
                     /tool="antismash"
     gene            3930..4148
                     /locus_tag="L604_RS19405"
                     /old_locus_tag="L604_000700001420"
     CDS             3930..4148
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664240.1"
                     /locus_tag="L604_RS19405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000700001420"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_042976883.1"
                     /transl_table=11
                     /translation="MNNNLKLERTRANLTQEQLAEKIGVTRQTIGLIEKGKYNPSLQLC
                     LKICIELKVTLNDVFWTYNREEESNEG"
     gene            4138..4563
                     /locus_tag="L604_RS19410"
                     /old_locus_tag="L604_000700001430"
     CDS             4138..4563
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042802.2"
                     /locus_tag="L604_RS19410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000700001430"
                     /product="DUF6773 family protein"
                     /protein_id="WP_042976881.1"
                     /transl_table=11
                     /translation="MKDERISHIENKIGHESFLLVIALVFVSFILSIMFKIPSTLMTII
                     ILFVGSGYYGLRAMSKGILRVNLKKKKKSSYFLLIVFTFILYMATSILFKIITFPFTTK
                     EFLFQILMYILWLIPFSLIMYTTYKFSNRQQKKELEN"
     misc_feature    complement(4564..4582)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: medium, score: 26.61"
                     /tool="antismash"
     misc_feature    4592..4610
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 24.61"
                     /tool="antismash"
     gene            4753..4983
                     /locus_tag="L604_RS19415"
                     /old_locus_tag="L604_000700001440"
     CDS             4753..4983
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021923.4"
                     /locus_tag="L604_RS19415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000700001440"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_042976879.1"
                     /transl_table=11
                     /translation="MDTKSIKDVELQALTLEEAEQIDGGSYAKYLGTSVVTGAGAGVVG
                     GVPGVILGAHAGAIAGSVGYLGGSLIAGKWL"
     gene            5001..5225
                     /locus_tag="L604_RS19420"
                     /old_locus_tag="L604_000700001450"
     CDS             5001..5225
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021923.4"
                     /locus_tag="L604_RS19420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000700001450"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_042976877.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.4e-09,
                     bitscore: 31.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENNNRFSELNVKEMEQIDGGGWRSALGGAFSGALVGASTVGVGG
                     AAVGGPVGGVGGAITGGIVGGIVGGINEW"
     gene            complement(5340..6161)
                     /gene="yxbG"
                     /locus_tag="L604_RS19425"
                     /old_locus_tag="L604_000700001460"
     CDS             complement(5340..6161)
                     /NRPS_PKS="Domain: PKS_KR (7-160). E-value: 1.4e-09. Score:
                     30.0. Matches aSDomain:
                     nrpspksdomains_L604_RS19425_PKS_KR.1"
                     /codon_start=1
                     /gene="yxbG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391863.2"
                     /locus_tag="L604_RS19425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000700001460"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_042976876.1"
                     /sec_met_domain="adh_short (E-value: 1.8e-33, bitscore:
                     109.6, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1e-37, bitscore:
                     124.0, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRLENKTAVITGAATGIGQATAEVFANEGARVMIGDINEDQMKE
                     TVEAIRQNGGQADAFHLDVSDENSVKAFADQIKEACGTIDILFNNAGVDQEGGKVHEYP
                     VDLFDRIIAVDLRGTFLCSKYLIPLMLEKGGSIINTSSMSGRAADLDRSGYNAAKGGIT
                     NLTKAMAIDYARDGIRVNSISPGTIETPLIDKLAGTKEQEMGEKFREANKWVTPLGRLG
                     QPKEMATVALFLASDDSSYVTGEDITADGGIMAYTWPGKMLLDEKWKEETK"
     aSDomain        complement(5682..6140)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS19425_PKS_KR.1"
                     /evalue="1.40E-09"
                     /label="L604_RS19425_PKS_KR.1"
                     /locus_tag="L604_RS19425"
                     /protein_end="160"
                     /protein_start="7"
                     /score="30.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TAVITGAATGIGQATAEVFANEGARVMIGDINEDQMKETVEAIRQ
                     NGGQADAFHLDVSDENSVKAFADQIKEACGTIDILFNNAGVDQEGGKVHEYPVDLFDRI
                     IAVDLRGTFLCSKYLIPLMLEKGGSIINTSSMSGRAADLDRSGYNAAKG"
     CDS_motif       complement(6066..6134)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS19425_0001"
                     /evalue="7.70E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="L604_RS19425"
                     /protein_end="32"
                     /protein_start="9"
                     /score="15.4"
                     /tool="antismash"
                     /translation="VITGAATGIGQATAEVFANEGAR"
     gene            6272..6523
                     /locus_tag="L604_RS19430"
     CDS             6272..6523
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391862.1"
                     /locus_tag="L604_RS19430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_042976874.1"
                     /transl_table=11
                     /translation="MKIQHKKELKFYCFVTIPSAFILLTLISFLFKEITFPVTASAFLN
                     ASWHNLLFLIPFGLFFYPVHIWMKREFGRWNDTEKKRG"
     gene            6691..8571
                     /gene="htpG"
                     /locus_tag="L604_RS19435"
                     /old_locus_tag="L604_000700001470"
     CDS             6691..8571
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /gene="htpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391861.1"
                     /locus_tag="L604_RS19435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000700001470"
                     /product="molecular chaperone HtpG"
                     /protein_id="WP_003243561.1"
                     /transl_table=11
                     /translation="MAKKEFKAESKRLLDMMINSIYTQKEIFLRELISNSSDAIDKIYY
                     KALTDDALTFDKDSYYIKVAADKDARTLTISDTGIGMTKDELEQHLGTIAKSGSLAFKK
                     ENELKDGHDIIGQFGVGFYAAFMVADVVTVISKALGSEEAYKWESAGADGYTIEPCEKD
                     SVGTDIILKIKENTEDDSYDEFLEEYRLKAIIKKYSDFIRYPIKMDTTINKPKEGSENE
                     FEEVQEEQTVNSMVPIWRKNKSELTDEDYEKFYAEKHYGFDKPLTHVHISVDGAVRYNA
                     ILFVPENMPFDYYSKEYEKGLELYSNGVLIMNKCADLLPDHFSFVKGMVDSEDLSLNIS
                     REMLQHDRQLKLIAKNISKKIKSELQSLLKKDREKYETFYKSFGRQLKFGVYNDFGANK
                     DQLKDLLLFYSSKEKKLVSLDEYVSRMPEEQKFIYYATGDSYDRIEKLPQTEMVADKGY
                     EILYFTEDIDEFAIKMLASYQEKEFKSVSSGDLGIDTDEDQKQSEEEESKYKDLFEEMK
                     NILSDKVKNVRISKRLKSHPVCLAADGEVTIEMEKILNAMPDSQNVKAEKVLEINPNHE
                     VFQTLQDAFEQDKEKLSLYTNLLYNQALLIEGLPIQDPVEFTNNICKVMV"
     gene            complement(8613..9998)
                     /gene="csbC"
                     /locus_tag="L604_RS19440"
                     /old_locus_tag="L604_000700001480"
     CDS             complement(8613..9998)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="csbC"
                     /gene_functions="transport (smcogs) SMCOG1169: sugar
                     transport protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391860.1"
                     /locus_tag="L604_RS19440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_000700001480"
                     /product="sugar porter family MFS transporter"
                     /protein_id="WP_042976872.1"
                     /transl_table=11
                     /translation="MKKDTRKYMIYFFGALGGLLYGYDTGVISGALLFINNDIPLTTLT
                     EGLVVSMLLLGAIFGSALSGTCSDRWGRRKVVFVLSIIFIIGALACAFSQTIGMLIASR
                     VMLGLAVGGSTALVPVYLSEMAPTKIRGTLGTMNNLMIVTGILLAYIVNYLFTPFEAWR
                     WMVGLAAVPAVLLLIGIAFMPESPRWLVKRGREEEARRIMNITHDPKDIEMELAEMKQG
                     EAEKKETTLSVLKAKWIRPMLLIGVGLAIFQQAVGINTVIYYAPTIFTKAGLGTSASAL
                     GTMGIGILNVIMCITAMILIDRVGRKKLLIWGSVGITLSLAALSGVLLTLGLSASTAWM
                     TVVFLGVYIVFYQATWGPVVWVLMPELFPSKARGAATGFTTLVLSAANLIVSLVFPLML
                     SAMGIAWVFMVFSVICLLSFFFAFYMVPETKGKSLEEIEASLKKRFKKKKSTQNQVLNE
                     RTL"
     misc_feature    10086..10099
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.21"
                     /tool="antismash"
ORIGIN
        1 taactttatt tttctctatt gtgctttcag caatataaaa tttttgtccg ttttgatcac
       61 taatactgtc tggacttatt tttgtaatga cacctttgat ggtttgattt gtattttcaa
      121 tatggaatct tacttcatct ttttcattaa tatttgtgat ctcatgactc ggaatataaa
      181 tattagcttt aagcttctct ttatcaggga taatagtcaa aatagcagtg cctgcttgta
      241 aattgtcacc tggagatata tttgttagta cattaacata gccgtcactt gctgctttta
      301 ttttatattt ttctaattgt aaatctatca tctttagatc atcctctttt gatttaagag
      361 tggtctgaag atctgtttgc ttttgagatg tctgatttag caagtttgtt ttgagctgtt
      421 caatagaaag cttataattt gtttcctctg agttatcgga atcactgtca gaatcgtcac
      481 ttttaattga actgttaatc tcatcaagag attgatttaa ttcgtctttt tcttcactta
      541 gttttactaa catatcttgt tttaaacttt caagctgtcc gttcaagttc tctttctgct
      601 gtgaaaaacg gtttttatcg agctcatctt tcatcttccg tagatcatca agttgctgct
      661 ctacattttg tttgttagtt tgataactat tatagtaaga actgtactta tcatcctgtt
      721 tggcaaagga tttattgttt ttaatggagt cttgcagatc agaaattctt tgaagcttta
      781 gtttgacttt gttttttgtc ttatccagac tatcttttat gctttttttc tgcttcttaa
      841 gttttttctg ctgagttttt gcattttctt ttgatgactc taacaaaata ctttgttttt
      901 cgtacgagag atattgatct tgataacccc aatgatcgtc ctcactgaat aaatttttgt
      961 cattttcaat actttttttt agagttttgt ttaacgcagc ttgttctttt aaatctttga
     1021 tatcagcttt aacagctttt ttctgcgctt ggatttcagt attgtctaga gttaatagag
     1081 tatctccctt tttcacctta tcaacctgtt taatatcaac tgtttttact ttgccggcta
     1141 cttcgtttgt tacgttgctg ttaggcttgg tatattggat gtcagctgtc gtttttagaa
     1201 tcacttcttt ctttccatag aaactaaaaa caatacatgc aatcagtaaa gctatcagta
     1261 tatatgtaaa gaaaacacca attggtttgg gttttgattc atagaattct ctgctatcct
     1321 tcaaatcatg taaattgaaa aagttacgtt tcatctaaat agtcaccatc cctctggatg
     1381 gcatttggtt ctcccacaac tcatagtact tgcctttttt cttcaataaa tcattgtggt
     1441 tcccatactc tatgatttct ccattttcca tgacaaatat tttgtcacaa ttcataattg
     1501 tactcaaacg gtgagcgatt ataatcgtag ttataccaac agtctgatcg tatatcattt
     1561 ttgatattcc ctgttcggta gtcgtatcga gattgcttgt tgattcatcc attatgagaa
     1621 tgttaggttt tcttaataat gctctggcaa ttgcaagtct ttgcttctgg cctccagata
     1681 aattagaccc gttttcctct ataagagtat cgtatctcat tggtaaatca ttgataaaat
     1741 catgtacttt agtttgttta catacctcta taatctcttc aaaggtaaaa ctgcctgagt
     1801 tcccaagcaa aaggttttca agaattgatc cttgtataaa atgagattct tgagggatat
     1861 aacttatact ttcccttaac gcgttggtgt gaatatcatt tatatgataa ccattgatta
     1921 aaacctcacc tgattcagct gtataaaatt tcattaaaag cttagtcaaa gtagattttc
     1981 ctgaaccgct ttctccaaca aaggcaactt tctctccttt ttgaatatgc agtgtaatat
     2041 cttttaagat gagccttcga gttccgtatc gaaaattgat tttattaaaa gaaagcggac
     2101 ctgtaattga cgacgtcgat gttatttgtg cctctttctc ttttaattcg ggttctagct
     2161 ttagaatttc tccgagcctt ttggacgcaa cagaagctga ttgaagctta gattgtaggc
     2221 cgatgatgtt ttgtaaagga tttaagaagt aggttaataa ggcattatat gtaataagct
     2281 gaccgatagt aagggtgcct ttcatgacct gagttgatcc gatccacagt atcaatgcac
     2341 cgcttgctaa atccaaaaac atttttatgg aactctgaag attatcaata actccgtgtt
     2401 taaaagctga tcgtaagtat ttcacaaatc ttttttctgt ttcaaatgac atcatatatt
     2461 ccgcattgta tgctttaaca gtttcgatgc cgttaagcgt ctcaacaatc tttgaagtca
     2521 gctgcgcatt gttttccatt tctgtgttat ttatattttc gtagggtttt ataaatatcc
     2581 aaaccgtcaa aatataaaaa ggtatgaaag ccaatgtgat gagaaataaa gtagtattct
     2641 gcagatataa gaaaatacca accataataa gcataatggt atcgagtatg accgaaagcg
     2701 atgcactgac aactgcatcg atcactttat tagcatcagt aaatctgata taatttctcc
     2761 gacttttcga ttactgaaaa atttcattgg caaagtaagc acatgttgaa agtatccaaa
     2821 cataatggag atacttaatt tttgtcctag gagaatcatt aggtattgcc taaaacctat
     2881 taagaaaacc ttaaataaat acataatcaa aaggccgatt gaaagaatat gtaatgtgtt
     2941 acttaatcca ttaggcagga tttgatcgat gatcgtctga aagtaaaaag taccgaggat
     3001 cccgaagatg gtaataataa aggatgctaa aaacgtatgt ataatcaacg atttttgctg
     3061 aagaatcaac ctgaaaatat cggcacttat attacttgtt tcggtctgtt ttttataagt
     3121 ctcagtaggt gctaaaagta ttaagatgcc tgtccaaatc ttaaaaaaat catctttttg
     3181 atatgtaacg agcccttttc caggatccgc aatgataatc ttatctttac taattttatg
     3241 tataacaacg taatgcaata attgctgatc tataataata tgtgcaattg ctggaagcgt
     3301 aatggactga tcaaaagctg tatgctctgc acgcacggct tttgctgaaa accccaattg
     3361 ttcggcggct tttaccattc caagagcact agtaccttgt gtatcagttc cagcaatgtc
     3421 acgtatataa gctagggaga gctttgaacc ataatgtttt gcaattgttg ataggcaggc
     3481 tgctccacaa tctttttgat caagttgtcg tacacaatga tatctattaa aaaacatata
     3541 ctactctcca taatgaaggt cattttttag aatagacatc attttattac attttcaggg
     3601 tttgtcaacc tttttcaggt aaatatttat tgctatttag agcttttggc acgtttatat
     3661 aatataaatg cgtagttgat atagataaga tataaagcaa gattaaatac acctaagtga
     3721 aaaaaataag aaagagatat acaaaataaa ccccctgcga ttatatagct atagaacaat
     3781 ttcattttac caactcctat aaatgttaaa ttcattgtaa gatataatgg acaaaatgta
     3841 aaatatatat ggataaatgc aatatatagt ttgcatggtt ttttcatgta tgatatgcta
     3901 cagataaata aaccaatgga gacttgccaa tgaataataa tttgaagttg gaaagaacaa
     3961 gagcgaattt aacacaggaa caacttgccg aaaaaattgg agtgacaaga cagacgattg
     4021 gattaattga aaaagggaag tataatcctt ctttacaatt atgtttgaaa atttgtatag
     4081 aattgaaagt tactctgaat gatgtatttt ggacgtataa cagggaggaa gaatctaatg
     4141 aaggatgaga gaataagtca tattgaaaat aagataggtc atgaaagctt tcttttagta
     4201 attgctttgg tttttgtctc ttttatactt tcgattatgt ttaaaatacc gagtacgtta
     4261 atgacaatta tcatattgtt tgttggaagc ggatattatg gattaagagc tatgtcaaaa
     4321 ggtattttac gagtcaatct gaaaaagaag aagaaaagtt cgtactttct tttaattgtt
     4381 tttactttta ttctgtatat ggcaacgtcg atattattta aaatcattac atttcctttt
     4441 acaactaaag aatttttatt ccaaattctt atgtatatct tatggctaat tccttttagt
     4501 ctcatcatgt atactacata taaattttca aacaggcagc agaaaaaaga attagagaat
     4561 taattaagag agcttaacct aaataactga attaggttaa gctctctttt actatatata
     4621 aattgatgat aggttatata tattttttat ggaaaaaact attgatttag gatttattat
     4681 gtaatatcat aatccgcgtg gtaaatttat actagtaatt atttacctga ataacttcga
     4741 ggaggaatat tgatggacac aaaaagcata aaagacgttg aacttcaggc attgactctt
     4801 gaagaagctg aacaaattga tggaggcagt tatgcgaaat atcttggcac ttctgttgtt
     4861 acaggtgccg gtgcgggtgt agttggagga gttccaggtg ttattcttgg tgcccatgca
     4921 ggtgcaattg caggatcagt tggttattta ggcggatcat taattgcagg aaaatggctt
     4981 taaggaggga taacacatca atggaaaata ataatcgttt ttctgaatta aacgtaaaag
     5041 aaatggaaca aattgatggc ggaggctgga gaagtgctct tggcggagct ttttccggag
     5101 cattagtcgg tgcttcaaca gtaggcgtcg gtggagcagc tgttggcggc cctgttggcg
     5161 gtgtcggcgg agctattact ggcggtatag ttggcggcat tgtcggcgga atcaatgaat
     5221 ggtgaaaaat acataaacga ctagtttagt actatggtat acgaccggca agagtcttct
     5281 cttcccggtc gtattatatt ttataattgt taattaacgc ataaaattgc gcagcatgtt
     5341 tatttcgtct cctccttcca tttctcatct aacagcatct tcccaggcca tgtatacgcc
     5401 atgatgccgc cgtctgcggt gatgtcttct cctgtgacgt atgagctgtc gtctgaagcg
     5461 aggaacagcg ccaccgtcgc catttctttc ggctggccga ggcgtccgag cggtgtgacc
     5521 cacttgtttg cttcgcggaa cttttcgccc atttcctgtt ctttggtgcc cgctagttta
     5581 tcaatcagcg gggtttcgat cgtacccggc gaaatggaat tgacgcggat tccgtcccgt
     5641 gcgtagtcaa tcgccatcgc ttttgtcagg tttgtgatgc cgccttttgc tgcgttgtag
     5701 ccggagcggt caaggtccgc ggcccggcct gacatggagg aggtgttgat gatggagcct
     5761 cctttttcga gcatgagcgg aatcagatat ttgctgcaaa ggaatgtgcc gcgcaggtcg
     5821 acggcgataa tgcggtcaaa caggtcaacc ggatattcgt gcacctttcc gccttcctga
     5881 tcaacgccgg cgttattaaa caaaatatca atcgttccgc acgcctcctt gatttgatca
     5941 gcaaaagctt tcacgctgtt ttcatctgac acatcgaggt gaaaggcgtc cgcctgtcct
     6001 ccattttgtc tgattgcttc aactgtttct ttcatttggt cttcattgat gtccccgatc
     6061 atcacacgcg cgccttcatt ggcaaaaacc tctgccgtcg cttgtccaat gcctgtcgcg
     6121 gcgcctgtga tcactgcggt tttgttttca agtcttccca tgacacgacc tcctttttct
     6181 atttgtcttc ccgctctgtg cagtcataaa catgcgagaa aggaaatgca tgtgttaaga
     6241 taggctggaa attggttggg gggtgtgtga gttgaaaatc cagcataaga aggagctcaa
     6301 gttttattgc ttcgttacaa ttccatctgc atttattctg ctgactctca tctcgtttct
     6361 attcaaagag attacatttc cagttacagc aagcgcgttt ctaaatgctt catggcataa
     6421 tctattattt ttgataccgt tcggtttgtt tttctatccg gtgcatattt ggatgaagcg
     6481 ggagtttggc cgctggaatg acacagaaaa aaagagagga tgatgaagcc tctctttttt
     6541 atgagaatga gtgagaaaaa gagtgaaaac aaatccaact aatgttaaat ttacatatat
     6601 atgcatctat caatctaatt gacaattgtc atcttatgtg ataaatagat gctgaaaagg
     6661 gtttacataa tggtaaagga gcgatcaaga gtggcaaaaa aagagtttaa agcagagtct
     6721 aaacgcttgc tggatatgat gattaactcc atttataccc aaaaagaaat tttcctgcgc
     6781 gagttaatct ccaattcgag tgatgcgatt gacaaaatct attacaaagc gcttacggac
     6841 gatgcgctta cgtttgataa agacagttac tacataaagg tcgcagctga caaagatgcg
     6901 agaacattaa ccatctctga tacgggtatc ggaatgacaa aggatgagct tgaacagcat
     6961 cttggaacaa ttgcaaaaag cggttccctc gcgtttaaaa aggaaaacga gctgaaagac
     7021 ggacacgaca tcatcggcca attcggtgtc ggtttttacg cggcatttat ggttgccgat
     7081 gtcgtgacag tcatcagtaa agcgctgggc agcgaagagg cttataaatg ggagtctgcg
     7141 ggtgcagacg gctatacgat tgaaccgtgt gaaaaggatt ctgtcggtac agacattatc
     7201 ctgaaaatca aagagaacac agaagatgac agctatgacg agtttttaga ggaatatcgt
     7261 ttaaaagcga tcatcaagaa gtactccgac ttcattcgct acccgatcaa aatggataca
     7321 accattaata agccgaagga aggcagcgaa aatgaatttg aagaggtgca ggaagagcag
     7381 acggtcaaca gcatggtgcc gatctggaga aaaaacaaaa gcgagctgac agatgaggat
     7441 tatgaaaaat tctatgctga aaagcattac gggtttgata aaccgctcac acacgttcat
     7501 atcagtgtag atggagccgt tcgctacaac gcgattctat tcgtccctga aaacatgccg
     7561 tttgactact attccaagga atatgagaaa ggcttggagc tgtattccaa tggcgtgctg
     7621 atcatgaata agtgcgcaga cctgctaccg gaccacttca gctttgtgaa gggcatggtc
     7681 gattctgagg atctatcgct gaacatttcc agagaaatgc tgcagcatga ccgccagctg
     7741 aagctgatcg cgaaaaacat cagcaagaaa atcaaaagcg agctgcaaag cctgttgaaa
     7801 aaagaccgcg agaaatacga gacgttctac aagtcgttcg gcagacagct gaaattcggc
     7861 gtgtacaatg attttggcgc gaataaggat caattgaagg atctcctctt attctactct
     7921 tcaaaagaga aaaaactggt cagcttagac gagtatgttt ccagaatgcc agaggagcag
     7981 aaattcattt attacgcgac cggtgattct tacgaccgca ttgagaaact gccgcaaacg
     8041 gaaatggtgg cggacaaagg ctatgaaatc ctctacttta ctgaggacat cgatgagttt
     8101 gcaattaaaa tgctggcttc ttatcaggaa aaagaattca agtccgtatc aagcggagac
     8161 cttggcatag acacggatga ggatcaaaag cagtccgagg aagaggaaag caagtataag
     8221 gatctgtttg aggaaatgaa aaacattctg tcagacaaag tgaaaaacgt cagaatctca
     8281 aaacggctta aatcacatcc agtctgcctt gccgctgacg gcgaagtgac aattgagatg
     8341 gaaaaaatct taaatgcgat gcctgacagc caaaatgtga aggcggaaaa agtgctggaa
     8401 atcaacccaa atcacgaggt attccaaaca ctgcaggacg cgtttgaaca agataaagag
     8461 aagctgagcc tgtacacaaa ccttctgtac aatcaggcgc tcctcatcga aggcctgccg
     8521 attcaggacc ctgtggaatt tacgaacaat atttgcaagg tcatggtgta aacagaaaaa
     8581 ggaatcgtcc cataaggaga cgattccttt ttttataatg tacgctcatt cagcacttgg
     8641 ttttgggtgc ttttcttctt tttaaaccgt tttttcaggc ttgcttcaat ttcctccaag
     8701 cttttccctt tggtttccgg taccatgtag aacgcgaaga aaaatgagag aagacaaatg
     8761 actgagaaaa ccataaagac ccatgcgatg cccatcgcgc ttagcatcag cggaaagaca
     8821 agcgacacga tcaggttagc cgccgataac accaaagtcg tgaaccctgt cgcggctccg
     8881 cgtgctttgg acgggaagag ctcaggcatg agcacccaca ctaccggacc ccatgtcgcc
     8941 tggtaaaaga cgatatagac acctagaaag actactgtca tccacgctgt tgatgcggaa
     9001 agtccgagcg ttaggagcac gcctgataaa gcagcaaggc tcagtgtgat gccgacactt
     9061 ccccagatca gaagtttttt gcggccgaca cggtcaatta agatcatggc agtgatacac
     9121 atgatgacgt tgaggatgcc gatccccatt gtgccgagcg ccgaagctga tgtgccaaga
     9181 ccggccttcg taaaaatggt tggcgcgtag tagatgaccg tgttaatgcc gaccgcctgc
     9241 tggaaaatgg caaggccgac gccaatcaga agcatggggc gaatccattt ggcttttagg
     9301 acgctgagtg tcgtttcttt tttctctgct tctccttgct tcatctctgc caattccatt
     9361 tcgatgtctt ttggatcatg agtgatgttc atgattcgtc tcgcttcttc ttcacggccg
     9421 cgcttcacga gccatcttgg cgattccggc atgaacgcga tgccgattaa taaaagcacg
     9481 gcaggcactg cggccagacc gaccatccag cgccacgctt caaacggtgt gaacagatag
     9541 ttaacgatat aggcgagcaa aatcccggtg acaatcatta agttgttcat tgtgccgagt
     9601 gtcccgcgaa ttttcgtcgg tgccatttcg gaaagataca cgggcacaag cgccgttgaa
     9661 ccgccgacgg ccaacccgag catcacccta gatgcaatca gcatgccgat tgtttgagaa
     9721 aacgcgcaag cgagcgctcc gataatgaaa atgatggaaa ggacaaatac aacctttctc
     9781 ctgccccagc ggtcggagca tgtgccgctc aaggcagaac cgaaaatcgc gccaaggagc
     9841 agcatgctga caaccaatcc ttctgtcaat gtcgttaaag gaatatcgtt attgataaac
     9901 aggagtgctc cggagataac acctgtatcg taaccatata gcagtccccc taaagcgccg
     9961 aaaaagtaga tcatatattt ccttgtgtct ttcttcacaa atgtctcctc ctcagttgtt
    10021 gagccaatga gtatctaaaa tgtataacaa agttaataga ttagtaccca tttcctatct
    10081 catttgaaac atttgttttg aaaaaacgga aacgttaaag ccaaaagaga tgatcacaga
    10141 cccgctgagt ttaggaaaaa cgaaataact ttatgctcag agaaaccatt aaaaaaacct
    10201 tacccgcgcg gagtaaggtt ttgat
//
