LOCUS       NZ_VLJL01000031         9497 bp    DNA     linear   CON 03-JUL-2024
DEFINITION  Bacillus subtilis J27 BacJ27_scaffold_36, whole genome shotgun
            sequence.
ACCESSION   NZ_VLJL01000031
VERSION     NZ_VLJL01000031.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus subtilis J27
  ORGANISM  Bacillus subtilis J27
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Gladden,J.
  TITLE     Genome sequencing of lignin-degrading bacterial isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Gladden,J., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.0.19
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 510.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007829735.1-RS_2024_07_03
            Annotation Date                   :: 07/03/2024 03:39:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,202
            CDSs (total)                      :: 4,129
            Genes (coding)                    :: 3,934
            CDSs (with protein)               :: 3,934
            Genes (RNA)                       :: 73
            rRNAs                             :: 5, 5, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 5, 1 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 195
            CDSs (without protein)            :: 195
            Pseudo Genes (ambiguous residues) :: 0 of 195
            Pseudo Genes (frameshifted)       :: 128 of 195
            Pseudo Genes (incomplete)         :: 68 of 195
            Pseudo Genes (internal stop)      :: 26 of 195
            Pseudo Genes (multiple problems)  :: 24 of 195
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:40:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9497
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VLJL01000031.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..9497
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1827:>7923](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1828..>7923
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     gene            <1..1746
                     /locus_tag="L604_RS14595"
                     /old_locus_tag="L604_003700000010"
     CDS             <1..1746
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (37-105). E-value: 8e-28. Score:
                     88.4. Matches aSDomain: nrpspksdomains_L604_RS14595_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (115-418). E-value:
                     8e-179. Score: 587.9. Matches aSDomain:
                     nrpspksdomains_L604_RS14595_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (423-577). E-value: 6e-56.
                     Score: 180.5. Matches aSDomain:
                     nrpspksdomains_L604_RS14595_TIGR01720.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388230.2"
                     /locus_tag="L604_RS14595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="L604_003700000010"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_252183850.1"
                     /sec_met_domain="PP-binding (E-value: 1.6e-18, bitscore:
                     55.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.6e-98, bitscore:
                     317.9, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TLNELPVTANGKVDRKALPEPDIEAGSGEYKAPTTDMEELLAGIW
                     QDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQHGWKLEMKDLFQHPTIEXLTQYVER
                     AEGKQADQGPVEGEVILTPIQRWFFEKNFTNKHHWNQSVMLHAAKGFDPERVEKTLQAL
                     IEHHDALRMVYREGQEDVIQYNRGLEAASAQLEVIQIEGQAADYEDRIEREAERLQSSI
                     DLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPVLPQ
                     KTHSFAEYAERLQDFANSKAFLKEKEYWRQLEEQAVAAKLPKDRESGDQRMKHTKTIEF
                     SLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVNVHLEGHGREEIIEDL
                     TISRTVGWFTSMYPMVLDMKHADDLGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKE
                     DVAFSIQPDVSFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKL
                     TMSLAYHSLEFHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDK
                     LMEIF"
     source          1..9497
                     /db_xref="taxon:935859"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis J27"
                     /strain="J27"
                     /submitter_seqid="BacJ27_scaffold_36"
     region          1..9497
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9497
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     aSDomain        115..318
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS14595_PCP.1"
                     /evalue="8.00E-28"
                     /label="L604_RS14595_PCP.1"
                     /locus_tag="L604_RS14595"
                     /protein_end="105"
                     /protein_start="37"
                     /score="88.4"
                     /tool="antismash"
                     /translation="EELLAGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQHGW
                     KLEMKDLFQHPTIEXLTQYVERA"
     aSModule        115..1734
                     /domains="nrpspksdomains_L604_RS14595_PCP.1"
                     /domains="nrpspksdomains_L604_RS14595_Epimerization.1"
                     /domains="nrpspksdomains_L604_RS14595_TIGR01720.1"
                     /incomplete
                     /locus_tags="L604_RS14595"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        349..1257
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS14595_Epimerization.1"
                     /evalue="8.00E-179"
                     /label="L604_RS14595_Epimerization.1"
                     /locus_tag="L604_RS14595"
                     /protein_end="418"
                     /protein_start="115"
                     /score="587.9"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EGEVILTPIQRWFFEKNFTNKHHWNQSVMLHAAKGFDPERVEKTL
                     QALIEHHDALRMVYREGQEDVIQYNRGLEAASAQLEVIQIEGQAADYEDRIEREAERLQ
                     SSIDLQEGGLLKAGLFQAEDGDHLLLAIHHLVVDGVSWRILLEDFAAVYTQLEQGNEPV
                     LPQKTHSFAEYAERLQDFANSKAFLKEKEYWRQLEEQAVAAKLPKDRESGDQRMKHTKT
                     IEFSLTAEETEQLTTKVHEAYHTEMNDILLTAFGLAMKEWTGQDRVNVHLEGHGREEII
                     EDLTISRTVGWFTSMYPMVLDM"
     CDS_motif       364..393
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS14595_0001"
                     /evalue="3.60E-05"
                     /label="NRPS-E1"
                     /locus_tag="L604_RS14595"
                     /protein_end="130"
                     /protein_start="120"
                     /score="16.4"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     CDS_motif       454..528
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS14595_0002"
                     /evalue="2.10E-06"
                     /label="C2_LCL_024-062"
                     /locus_tag="L604_RS14595"
                     /protein_end="175"
                     /protein_start="150"
                     /score="20.2"
                     /tool="antismash"
                     /translation="FDPERVEKTLQALIEHHDALRMVYR"
     CDS_motif       730..795
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS14595_0003"
                     /evalue="1.90E-12"
                     /label="NRPS-E2"
                     /locus_tag="L604_RS14595"
                     /protein_end="264"
                     /protein_start="242"
                     /score="39.2"
                     /tool="antismash"
                     /translation="LLLAIHHLVVDGVSWRILLEDF"
     CDS_motif       1204..1251
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS14595_0004"
                     /evalue="1.00E-08"
                     /label="NRPS-E5"
                     /locus_tag="L604_RS14595"
                     /protein_end="416"
                     /protein_start="400"
                     /score="27.1"
                     /tool="antismash"
                     /translation="ISRTVGWFTSMYPMVL"
     aSDomain        1273..1734
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS14595_TIGR01720.1"
                     /evalue="6.00E-56"
                     /label="L604_RS14595_TIGR01720.1"
                     /locus_tag="L604_RS14595"
                     /protein_end="577"
                     /protein_start="423"
                     /score="180.5"
                     /tool="antismash"
                     /translation="LGYQLKQMKEDIRHVPNKGVGYGILRYLTAPEHKEDVAFSIQPDV
                     SFNYLGQFDEMSDAGLFTRSELPSGQSLSPETEKPNALDVVGYIENGKLTMSLAYHSLE
                     FHEKTVQTFSDSFKAHLLRIIEHCLSQDGTELTPSDLGDDDLTLDELDKL"
     CDS_motif       1297..1356
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS14595_0005"
                     /evalue="1.00E-09"
                     /label="NRPS-E6"
                     /locus_tag="L604_RS14595"
                     /protein_end="451"
                     /protein_start="431"
                     /score="30.6"
                     /tool="antismash"
                     /translation="KEDIRHVPNKGVGYGILRYL"
     aSDomain        1828..2673
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS21365_Condensation_DCL.1"
                     /domain_subtypes="Condensation_DCL"
                     /evalue="6.70E-104"
                     /label="L604_RS21365_Condensation_DCL.1"
                     /locus_tag="L604_RS21365"
                     /protein_end="282"
                     /protein_start="0"
                     /score="339.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIMMDG"
                     /tool="antismash"
                     /translation="MLDPHSSSYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFV
                     HQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHIERYKQDVQRQGFNLAKDILFKVA
                     VFRLAADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAGRTPANGQGKPYSDYIKW
                     LGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDEYVNKEYSFTWDETLVARIQQTAN
                     LHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGRPSEINGIETMAGLFINTIPVRVK
                     V"
     aSModule        1828..4797
                     /complete
                     /domains="nrpspksdomains_L604_RS21365_Condensation_DCL.1"
                     /domains="nrpspksdomains_L604_RS21365_AMP-binding.1"
                     /domains="nrpspksdomains_L604_RS21365_PCP.1"
                     /locus_tags="L604_RS21365"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     gene            1828..>4803
                     /gene="srfAB"
                     /locus_tag="L604_RS21365"
                     /pseudo=""
     CDS             1828..>4803
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_DCL (0-282). E-value:
                     6.7e-104. Score: 339.1. Matches aSDomain:
                     nrpspksdomains_L604_RS21365_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (447-841). E-value:
                     4.7e-111. Score: 363.2. Matches aSDomain:
                     nrpspksdomains_L604_RS21365_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (947-990). E-value: 7.2e-14. Score:
                     43.8. Matches aSDomain: nrpspksdomains_L604_RS21365_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="L604_RS21365"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.4e-83, bitscore:
                     268.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.3e-115, bitscore:
                     374.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.9e-09, bitscore:
                     25.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDPHSSSYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFV
                     HQQLQKPRQVVLAERKTKVHYEDISHADENRQKEHIERYKQDVQRQGFNLAKDILFKVA
                     VFRLAADQLYLVWSNHHIMMDGWSMGVLMKSLFQNYEALRAGRTPANGQGKPYSDYIKW
                     LGKQDNEEAESYWSERLAGFEQPSVLPGRLPVKKDEYVNKEYSFTWDETLVARIQQTAN
                     LHQVTGPNLFQAVWGIVLSKYNFTDDVVFGTVVSGRPSEINGIETMAGLFINTIPVRVK
                     VERDAAFADIFTAVQQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYPLDQELE
                     NGSMEDRLGFSIKVESAFEQTSFDFNLIVYPGKTWTVKIKYNGAAFDSAFIERTAEHLT
                     RMMEAAVDQPAAFVREYGLVGDEEQRQIVEVFNSMKAELPEGMAIHQVFEEQAKRTPAS
                     TAVVYEGTELTYRELNAAANRLARKLVEQGLQKGETAAIMNDRSVETVVGMLAXLKAGA
                     AYVPLDPALPEDRLRFMAVDSSIRIVLAGKSYTGQAHQLQVPVLTLDIGFEESEAADNL
                     NLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILRLVKNAGYVPVTEEDRMAQTGAVSFD
                     AGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLREQRISTMWLTSPLFNQLAAKDAGM
                     FGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYGPTENTTFSTSFLIDREYGGSIPI
                     GKPIGNSTAYILDEQQCLQPIGAPGELCVGGIGVARGYVNLPELTEKQFLEDPFRPGER
                     IYRTGDLARWLPDGNIEFLGRIDNQVKVRGFRIELGEIETKLNMAEHVTEAAVIIRKNK
                     ADENEICAYFTADREVAVSELRKTLSQSLPDYMVPAHLIQMDSLPLTPNGKINKKELPA
                     PQSEAVQPEYAAPETESEKKLAEIWEGILGVRAGVTDNFFMIGGHSLKAMMMTAKIQEH
                     FHK"
     CDS_motif       1849..1965
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21365_0001"
                     /evalue="2.70E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="L604_RS21365"
                     /protein_end="46"
                     /protein_start="7"
                     /score="45.4"
                     /tool="antismash"
                     /translation="SYFTQLELGIHGAFDLEIFEKSVNELIRSYDILRTVFVH"
     CDS_motif       2182..2247
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21365_0002"
                     /evalue="1.10E-11"
                     /label="C3_DCL_135-156"
                     /locus_tag="L604_RS21365"
                     /protein_end="140"
                     /protein_start="118"
                     /score="37.1"
                     /tool="antismash"
                     /translation="NHHIMMDGWSMGVLMKSLFQNY"
     CDS_motif       2293..2331
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21365_0003"
                     /evalue="1.10E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="L604_RS21365"
                     /protein_end="168"
                     /protein_start="155"
                     /score="11.5"
                     /tool="antismash"
                     /translation="PYSDYIKWLGKQD"
     CDS_motif       2569..2664
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21365_0004"
                     /evalue="7.20E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="L604_RS21365"
                     /protein_end="279"
                     /protein_start="247"
                     /score="63.4"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEINGIETMAGLFINTIPVR"
     CDS_motif       2713..2829
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21365_0005"
                     /evalue="1.60E-17"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="L604_RS21365"
                     /protein_end="334"
                     /protein_start="295"
                     /score="55.9"
                     /tool="antismash"
                     /translation="QQHAVEAERYDYVPLYEIQKRSALDGNLLNHLVAFENYP"
     aSDomain        3169..4350
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS21365_AMP-binding.1"
                     /evalue="4.70E-111"
                     /label="L604_RS21365_AMP-binding.1"
                     /locus_tag="L604_RS21365"
                     /protein_end="841"
                     /protein_start="447"
                     /score="363.2"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQAKRTPASTAVVYEGTELTYRELNAAANRLARKLVEQGLQKG
                     ETAAIMNDRSVETVVGMLAXLKAGAAYVPLDPALPEDRLRFMAVDSSIRIVLAGKSYTG
                     QAHQLQVPVLTLDIGFEESEAADNLNLPSAPSDLAYIMYTSGSTGKPKGVMIEHKSILR
                     LVKNAGYVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFL
                     REQRISTMWLTSPLFNQLAAKDAGMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNG
                     YGPTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYILDEQQCLQPIGAPGELCVGGIGV
                     ARGYVNLPELTEKQFLEDPFRPGERIYRTGDLARWLPDGNIEFLGRIDNQVKVR"
     CDS_motif       3355..3396
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21365_0006"
                     /evalue="4.00E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="L604_RS21365"
                     /protein_end="523"
                     /protein_start="509"
                     /score="16.8"
                     /tool="antismash"
                     /translation="LAXLKAGAAYVPLD"
     CDS_motif       3583..3642
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21365_0007"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="L604_RS21365"
                     /protein_end="605"
                     /protein_start="585"
                     /score="35.7"
                     /tool="antismash"
                     /translation="AYIMYTSGSTGKPKGVMIEH"
     CDS_motif       4144..4233
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21365_0008"
                     /evalue="2.60E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="L604_RS21365"
                     /protein_end="802"
                     /protein_start="772"
                     /score="48.5"
                     /tool="antismash"
                     /translation="PIGAPGELCVGGIGVARGYVNLPELTEKQF"
     CDS_motif       4315..4380
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21365_0009"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="L604_RS21365"
                     /protein_end="851"
                     /protein_start="829"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRIDNQVKVRGFRIELGEIE"
     aSDomain        4669..4797
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS21365_PCP.1"
                     /evalue="7.20E-14"
                     /label="L604_RS21365_PCP.1"
                     /locus_tag="L604_RS21365"
                     /protein_end="990"
                     /protein_start="947"
                     /score="43.8"
                     /tool="antismash"
                     /translation="EKKLAEIWEGILGVRAGVTDNFFMIGGHSLKAMMMTAKIQEHF"
     gene            4895..5035
                     /gene="comS"
                     /locus_tag="L604_RS21370"
     CDS             4895..5035
                     /codon_start=1
                     /gene="comS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388232.1"
                     /locus_tag="L604_RS21370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="competence protein ComS"
                     /protein_id="WP_010886404.1"
                     /transl_table=11
                     /translation="MNRSGKHLISSIILYPRPSGECISSISLDKQTQATTSPLYFCWRE
                     K"
     gene            <4981..>7923
                     /gene="srfAB"
                     /locus_tag="L604_RS21375"
                     /pseudo=""
     CDS             4981..>7923
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (2-264). E-value:
                     5.3e-94. Score: 306.5. Matches aSDomain:
                     nrpspksdomains_L604_RS21375_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (427-830). E-value:
                     3.1e-115. Score: 377.0. Matches aSDomain:
                     nrpspksdomains_L604_RS21375_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (936-979). E-value: 3.6e-17. Score:
                     54.3. Matches aSDomain: nrpspksdomains_L604_RS21375_PCP.1"
                     /codon_start=1
                     /gene="srfAB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003417.0"
                     /locus_tag="L604_RS21375"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="surfactin non-ribosomal peptide synthetase SrfAB"
                     /pseudo=""
                     /sec_met_domain="Condensation (E-value: 1.9e-85, bitscore:
                     274.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.8e-126, bitscore:
                     409.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.9e-10, bitscore:
                     29.7, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMID
                     GEVVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLL
                     IDMHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDV
                     FKGELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFN
                     VLLSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTAPAGDKTFAQFLEEVKTA
                     SLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNMQNMEIPSLRLGDLKISSYSMLH
                     HVAKFDLSLEAVEREEDIGLSFDYATALFKDETIRRWSRHFVNIIKAAAANPNVRLSDV
                     DLLSSAETAALLEERHMTQITEATFAALFEKQAQQTPDHPAVKAGGNLLTYRELDEQAN
                     QLAHHLRAQGAGNEDIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLED
                     SGAKIAVVNERNMTAIGQYEGTIVSLDDGKWRNESKERPSSISGSCNLAYVIYTSGTTG
                     KPKGVQIEHRNLTNYVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIV
                     QKETYTAPDEIAHYIKEHGITYIKLTPSLFHTIVNTAGFAKDANFESLRLIVLGGEKII
                     PTDVLAFRKMYGHTEFINHYGPTEATIGAIAGRVDLSEPDAFAKRPAIGRPIANAGALV
                     LNEALKLVPPGASGQLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRL
                     SDGTLAFIGRADDQVKIRGYRIEPKEIETVMLSLSGIQEAVVLAVSEGGLQELCAYYTS
                     DQAIEKAELRKQLSLTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNAAQSGGKALA
                     APETALEESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAALE"
     aSDomain        4987..5772
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS21375_Condensation_LCL.1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.30E-94"
                     /label="L604_RS21375_Condensation_LCL.1"
                     /locus_tag="L604_RS21375"
                     /protein_end="264"
                     /protein_start="2"
                     /score="306.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="NTSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSFDMIDGE
                     VVQTVHKNISFQLEAAKGREEDAEEIIKAFVQPFELNRAPLVRSKLVQLEEKRHLLLID
                     MHHIITDGSSTGILIGDLAKIYQGADLELPQIHYKDYAVWHKEQTNYQKDEEYWLDVFK
                     GELPILDLPADFERPAERSFAGERVMFGLDKQITAQIKSLMAETDTTMYMFLLAAFNVL
                     LSKYASQDDIIVGSPTAGRTHPDLQGVPGMFVNTVALRTA"
     aSModule        4987..7917
                     /complete
                     /domains="nrpspksdomains_L604_RS21375_Condensation_LCL.1"
                     /domains="nrpspksdomains_L604_RS21375_AMP-binding.1"
                     /domains="nrpspksdomains_L604_RS21375_PCP.1"
                     /locus_tags="L604_RS21375"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       4990..5103
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21375_0001"
                     /evalue="1.60E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="L604_RS21375"
                     /protein_end="41"
                     /protein_start="3"
                     /score="55.8"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGEVDKDRLENAIQQLINRHEILRTSF"
     CDS_motif       5299..5364
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21375_0002"
                     /evalue="8.20E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="L604_RS21375"
                     /protein_end="128"
                     /protein_start="106"
                     /score="28.0"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIGDLAKIY"
     CDS_motif       5395..5430
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21375_0003"
                     /evalue="1.20E-03"
                     /label="C4_LCL_164-176"
                     /locus_tag="L604_RS21375"
                     /protein_end="150"
                     /protein_start="138"
                     /score="11.6"
                     /tool="antismash"
                     /translation="HYKDYAVWHKEQ"
     CDS_motif       5677..5766
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21375_0004"
                     /evalue="9.00E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="L604_RS21375"
                     /protein_end="262"
                     /protein_start="232"
                     /score="50.2"
                     /tool="antismash"
                     /translation="DIIVGSPTAGRTHPDLQGVPGMFVNTVALR"
     CDS_motif       5821..5934
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21375_0005"
                     /evalue="2.40E-16"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="L604_RS21375"
                     /protein_end="318"
                     /protein_start="280"
                     /score="52.1"
                     /tool="antismash"
                     /translation="ASLQAFEHQSYPLEELIEKLPLTRDTSRSPLFSVMFNM"
     aSDomain        6262..7470
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS21375_AMP-binding.1"
                     /evalue="3.10E-115"
                     /label="L604_RS21375_AMP-binding.1"
                     /locus_tag="L604_RS21375"
                     /protein_end="830"
                     /protein_start="427"
                     /score="377.0"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEKQAQQTPDHPAVKAGGNLLTYRELDEQANQLAHHLRAQGAGNE
                     DIVAIVMDRSAEVMVSILGVMKAGAAFLPIDPDTPEERIRYSLEDSGAKIAVVNERNMT
                     AIGQYEGTIVSLDDGKWRNESKERPSSISGSCNLAYVIYTSGTTGKPKGVQIEHRNLTN
                     YVSWFSEEASLTENDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQKETYTAPDEIAHY
                     IKEHGITYIKLTPSLFHTIVNTAGFAKDANFESLRLIVLGGEKIIPTDVLAFRKMYGHT
                     EFINHYGPTEATIGAIAGRVDLSEPDAFAKRPAIGRPIANAGALVLNEALKLVPPGASG
                     QLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSDGTLAFIGRADDQ
                     VKIR"
     CDS_motif       6448..6489
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21375_0006"
                     /evalue="3.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="L604_RS21375"
                     /protein_end="503"
                     /protein_start="489"
                     /score="17.1"
                     /tool="antismash"
                     /translation="LGVMKAGAAFLPID"
     CDS_motif       6676..6735
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21375_0007"
                     /evalue="9.20E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="L604_RS21375"
                     /protein_end="585"
                     /protein_start="565"
                     /score="33.5"
                     /tool="antismash"
                     /translation="AYVIYTSGTTGKPKGVQIEH"
     CDS_motif       7264..7353
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21375_0008"
                     /evalue="4.20E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="L604_RS21375"
                     /protein_end="791"
                     /protein_start="761"
                     /score="51.0"
                     /tool="antismash"
                     /translation="PPGASGQLYITGQGLARGYLNRPQLTAERF"
     CDS_motif       7435..7500
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21375_0009"
                     /evalue="3.30E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="L604_RS21375"
                     /protein_end="840"
                     /protein_start="818"
                     /score="38.3"
                     /tool="antismash"
                     /translation="FIGRADDQVKIRGYRIEPKEIE"
     aSDomain        7789..7917
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS21375_PCP.1"
                     /evalue="3.60E-17"
                     /label="L604_RS21375_PCP.1"
                     /locus_tag="L604_RS21375"
                     /protein_end="979"
                     /protein_start="936"
                     /score="54.3"
                     /tool="antismash"
                     /translation="EESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQAA"
     aSDomain        8074..8901
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_L604_RS21380_Condensation_LCL.1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.90E-101"
                     /label="L604_RS21380_Condensation_LCL.1"
                     /locus_tag="L604_RS21380"
                     /protein_end="276"
                     /protein_start="0"
                     /score="329.3"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="MYVLNQLDRQTISYNMPSVLLMEGELDISRLRYSLNQLVNRHESL
                     RTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEADQKIKEFIRPFDLNDAPLIRAALLRI
                     EAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFAVWQNEAEQKER
                     MKEHEAYWMSVLSGELPELDLPLDYARPPVQSFKGDTIRFRTGSETAKAVEKLLAETGT
                     TLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRTNADVQDMPGMFVNTLALRMEA"
     gene            8074..>9497
                     /locus_tag="L604_RS21380"
     CDS             8074..>9497
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (0-276). E-value:
                     5.9e-101. Score: 329.3. Matches aSDomain:
                     nrpspksdomains_L604_RS21380_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="L604_RS21380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_252183851.1"
                     /sec_met_domain="Condensation (E-value: 1.6e-93, bitscore:
                     301.4, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYVLNQLDRQTISYNMPSVLLMEGELDISRLRYSLNQLVNRHESL
                     RTSFMEANGEPVQRIIEKAEVDLHVFEAKEDEADQKIKEFIRPFDLNDAPLIRAALLRI
                     EAKKHLLLLDMHHIIADGVSRGIFVKELALLYKGEQLPEPALHYKDFAVWQNEAEQKER
                     MKEHEAYWMSVLSGELPELDLPLDYARPPVQSFKGDTIRFRTGSETAKAVEKLLAETGT
                     TLHMVLHAVFHVFLSKISGQRDIVIGSVTAGRTNADVQDMPGMFVNTLALRMEAKEQQT
                     FAELLEQAKQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMVTTENPDKEQLTLQ
                     NLSISPYEAHQGTSKFDLTLGGFTDENGIGLQLEYATDLFAKETVEKWSEYVLRLLKAV
                     ADNPNQPLSSLSLVTETEKQALLEAWKGKALPVPTDKTVHQLFEETVQRHKDXPAVTYN
                     GQSWTYGELNAKANRLA"
     CDS_motif       8110..8220
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21380_0001"
                     /evalue="9.40E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="L604_RS21380"
                     /protein_end="49"
                     /protein_start="12"
                     /score="53.4"
                     /tool="antismash"
                     /translation="SYNMPSVLLMEGELDISRLRYSLNQLVNRHESLRTSF"
     CDS_motif       8416..8481
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21380_0002"
                     /evalue="7.50E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="L604_RS21380"
                     /protein_end="136"
                     /protein_start="114"
                     /score="31.3"
                     /tool="antismash"
                     /translation="MHHIIADGVSRGIFVKELALLY"
     CDS_motif       8512..8544
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21380_0003"
                     /evalue="5.10E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="L604_RS21380"
                     /protein_end="157"
                     /protein_start="146"
                     /score="15.8"
                     /tool="antismash"
                     /translation="HYKDFAVWQNE"
     CDS_motif       8803..8892
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21380_0004"
                     /evalue="4.40E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="L604_RS21380"
                     /protein_end="273"
                     /protein_start="243"
                     /score="48.0"
                     /tool="antismash"
                     /translation="DIVIGSVTAGRTNADVQDMPGMFVNTLALR"
     CDS_motif       8941..9054
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_L604_RS21380_0005"
                     /evalue="2.30E-18"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="L604_RS21380"
                     /protein_end="327"
                     /protein_start="289"
                     /score="58.5"
                     /tool="antismash"
                     /translation="KQTNLSALEHQEYPFEDLVNQLDLPRDMSRNPLFNVMV"
ORIGIN
        1 acgctgaacg agcttccggt tacggcaaac ggcaaagtgg accgcaaagc cttgcctgag
       61 ccggacatcg aagcgggaag cggagaatac aaagcgccga cgaccgacat ggaagagctg
      121 cttgccggca tctggcagga cgtgcttgga atgtctgaag tcggtgtcac cgacaatttc
      181 ttctcgcttg gcggagattc catcaaagga attcaaatgg cgagccgctt gaaccagcac
      241 ggctggaagc tggaaatgaa agatctcttc cagcanccga cgatcgaggn gcttacccaa
      301 tatgtagagc gtgccgaagg caaacaggca gancaaggcc cggtggaggg cgaagtcatc
      361 ctgacgccga tccagcgctg gttctttgaa aagaacttca cgaacaagca ccactggaac
      421 caatcggtga tgcttcacgc cgcgaagggc tttgaccctg aacgggtgga gaaaacattg
      481 caggcgctga tcgagcatca tgacgcgctc cgtatggtct accgtgaggg acaggaagac
      541 gtcattcaat acaacagagg tcttgaagct gcttccgctc aattggaggt catccagatt
      601 gagggccaag ctgcagatta cgaagaccgt atagagagag aagcggagcg tttgcaaagc
      661 agcatcgact tgcaggaagg cggcttgtta aaagcaggct tgttccaagc ggaagacgga
      721 gaccacttgc ttcttgccat tcaccactta gtggttgacg gcgtgtcgtg gcggatttta
      781 ctggaggatt tcgccgcagt atatacacag cttgagcaag gcaatgaacc ggttctcccg
      841 cagaaaacac attcatttgc agagtatgca gagagattgc aagacttcgc gaactccaaa
      901 gcctttttga aagaaaaaga gtattggaga cagcttgaag aacaagctgt cgcggcaaag
      961 cttccgaaag accgcgaatc tggtgatcag cgaatgaaac atacaaagac aattgaattc
     1021 tcgctgactg ctgaagagac agaacagctc accacaaagg tgcatgaggc atatcacaca
     1081 gaaatgaatg atattttgct gacggcattc ggattggcaa tgaaggagtg gacgggtcag
     1141 gatcgagtaa atgttcattt agaggggcac ggacgtgaag aaatcataga agacctgacc
     1201 atttcccgca cagtcggctg gtttacaagt atgtacccaa tggtgctcga tatgaagcat
     1261 gcggatgatc tgggctacca gctgaagcaa atgaaagaag atatcagaca tgtgccgaat
     1321 aagggagtcg gatacggcat tctgcgctat ctgacggcac cggaacataa agaagatgtg
     1381 gcgttctcga ttcagccgga tgtcagcttc aactatttag gtcagtttga cgaaatgtcg
     1441 gatgcaggtt tgtttacgag atcagagctg ccatcaggac agtcattaag ccctgaaaca
     1501 gaaaaaccga atgcgctgga tgttgtcgga tatatcgaaa acggaaaact gacgatgtca
     1561 ctggcctatc attctcttga atttcatgaa aaaacagtac aaacattcag tgacagcttt
     1621 aaagcgcatc ttctcagaat cattgaacat tgcctatctc aagacggtac ggaactgacc
     1681 ccgagtgatc ttggtgacga cgatttgacg ctggatgagc tggataaatt aatggaaatt
     1741 ttctaataga ggtggcatat gagcaaaaaa tcgattcaaa aggtgtacgc actgacacca
     1801 atgcaggagg gaatgctgta tcatgcgatg cttgatccgc attcttcctc gtacttcaca
     1861 caattagagc ttgggattca cggcgctttt gatcttgaaa tctttgagaa aagcgtcaat
     1921 gaactgattc ggtcatacga tattctccgt acggtatttg tgcatcagca gctgcaaaaa
     1981 ccgcgtcagg tcgtgttagc ggaacgcaaa acaaaggtgc attatgagga tatcagtcat
     2041 gcagacgaga accgccagaa agagcacatt gagcgttaca aacaagacgt tcagcgccaa
     2101 ggctttaacc tggcaaaaga catattgttc aaggtagcgg ttttccgcct tgctgcagat
     2161 cagctgtatc ttgtctggag caatcatcat attatgatgg acggctggag catgggcgtc
     2221 ctcatgaaaa gcctgttcca aaactatgaa gcgctcagag caggaaggac acctgcaaac
     2281 ggtcaaggca agccttactc cgactacatc aagtggcttg gaaaacagga caatgaagaa
     2341 gcggagagct actggagcga gcgcttggcg ggttttgaac aaccaagcgt gctcccgggc
     2401 cgcctgcctg tgaaaaaaga cgaatacgtc aataaagaat attcctttac atgggacgaa
     2461 acattggttg cccgtattca gcaaaccgca aatctccatc aagtgacagg gcctaaccta
     2521 tttcaggccg tttggggcat tgttctcagc aaatacaact ttacggatga tgttgtcttc
     2581 ggaacggtcg tctcaggaag gccgtctgaa atcaacggaa ttgaaacgat ggcggggctg
     2641 tttatcaaca ccattccagt gcgggtgaaa gttgaacgag atgctgcatt cgctgatatt
     2701 ttcacagctg ttcagcagca tgcagtagag gcagagcgtt acgattacgt gccgctctat
     2761 gagattcaaa aacgctcagc ccttgatggc aatctcttaa accatctggt cgcgtttgaa
     2821 aattatccgc ttgatcaaga gcttgaaaac ggcagcatgg aagaccgcct cgggttttcg
     2881 attaaggtag aaagcgcatt tgaacaaacg agcttcgatt tcaacctgat tgtgtatcca
     2941 ggcaaaacgt ggaccgtcaa aattaaatat aacggagccg cctttgattc cgcttttatc
     3001 gaacggacag cggagcacct tacccgcatg atggaagcag ctgtcgatca gccggccgct
     3061 tttgtgcgtg agtacgggct tgtgggagac gaagagcagc gtcaaattgt cgaggtgttt
     3121 aacagcatga aagccgaact ccctgaagga atggctattc atcaggtgtt tgaagagcaa
     3181 gcaaaacgga cgccggcgag cactgccgtc gtatatgaag gaaccgagct gacgtaccgc
     3241 gagctgaatg cagcggctaa ccgtctggcg agaaagcttg tcgaacaagg ccttcaaaaa
     3301 ggggaaacag cagcgattat gaacgatcgc tcagtagaaa ccgttgtcgg catgctggct
     3361 gntttaaaag caggcgccgc atatgtgccg cttgatccag cgcttcctga ggatcgtctt
     3421 cgtttcatgg cagtggacag ttccattcgg attgtattgg ccggaaagtc ttatacaggg
     3481 caggctcatc agctgcaagt gccggttctt acactggaca taggctttga agagagtgaa
     3541 gcggctgaca atctcaacct gccatccgcc ccgtctgatt tggcgtacat catgtacaca
     3601 tccggttcaa cgggcaaacc gaaaggcgtc atgatcgaac ataaaagcat tctgcgcctc
     3661 gtcaaaaatg ccgggtacgt tcctgttact gaagaagacc gcatggcgca aacgggagct
     3721 gtcagctttg atgccggaac gtttgaggta ttcggcgcac tgctgaatgg cgcagcgctt
     3781 tatccggtca aaaaagagac gctgcttgat gcgaaacaat ttgctgcatt tctgcgtgag
     3841 caaagaatct cgacgatgtg gctgacatca cctttattca accagcttgc tgcaaaggat
     3901 gcgggtatgt tcggcacact gcgccattta atcatcggcg gagatgccct tgtcccgcat
     3961 attgtcagca aagtaaaaca ggcatcgccg tcgctttcat tgtggaacgg atacggcccg
     4021 acagaaaaca cgacattttc aaccagtttt ctgatcgacc gcgagtatgg cggctctatc
     4081 ccaatcggga agccgatcgg aaattccact gcttacatcc tggatgagca gcaatgcctg
     4141 cagccaattg gcgcgcctgg tgagctttgc gtaggcggaa tcggtgtagc gcgtgggtat
     4201 gtcaatctcc ctgagctgac agagaagcaa tttctcgaag atccgttcag accgggtgag
     4261 agaatttatc gcactggtga cttggcaaga tggctgccgg acggcaatat cgaattttta
     4321 ggcagaatcg acaaccaagt gaaggtgcgc ggcttccgaa ttgagcttgg cgaaattgaa
     4381 acaaaactga acatggctga gcatgtgaca gaggctgcgg tgatcatccg caagaacaaa
     4441 gcggatgaaa atgaaatttg cgcgtacttt acggcggacc gtgaagtggc tgtgagcgag
     4501 ctgagaaaaa cactgtctca gtctttgcct gactatatgg tccctgccca cctgattcaa
     4561 atggacagtc tgccgctcac gccaaacggg aaaatcaaca aaaaagaact gcctgcaccg
     4621 caatcagaag ccgtgcagcc ggaatacgca gcaccagaaa cagagagtga aaagaaatta
     4681 gcggagatct gggaaggaat actcggcgtc agagcaggcg ttaccgataa cttctttatg
     4741 atcggcggcc attctttgaa agcgatgatg atgacggcga aaattcaaga gcattttcat
     4801 aaggaagttc cgataaaagt gctttttgaa aagccgacta ttcaagaact ggcactgtat
     4861 ttggaagaga acgaaagcaa ggaggagcag acgtttgaac cgatcaggca agcatcttat
     4921 cagcagcatt atcctgtatc cccggcccag cggagaatgt atatcctcaa tcagcttgga
     4981 caagcaaaca caagctacaa cgtccccgct gtacttctgc tggagggaga agtagataaa
     5041 gaccggcttg aaaacgcgat tcagcaatta atcaaccggc acgaaatcct ccgtacatcg
     5101 tttgacatga tcgacggaga ggttgtgcaa accgttcata aaaacatatc gttccagctg
     5161 gaggctgcca agggacggga agaagacgcg gaagagataa tcaaagcatt tgttcagccg
     5221 tttgaattaa accgcgcgcc gctcgtccgt tcgaagcttg tccagctgga agaaaaacgc
     5281 cacctgctgc tcattgatat gcatcatatt attactgacg gaagttcaac aggcattcta
     5341 atcggtgatc ttgccaaaat atatcaaggc gcagatctgg aactgccaca aattcactat
     5401 aaagattacg cagtttggca caaagaacaa actaattatc aaaaagatga ggaatactgg
     5461 ctcgatgtct ttaaaggcga actgccaata ctggatcttc ccgcggattt cgagcggcca
     5521 gctgaacgga gctttgcggg agagcgcgtg atgtttgggc ttgataagca aatcacggct
     5581 caaatcaaat cgctcatggc agaaacagat acgacaatgt acatgttttt gctggcggcg
     5641 ttcaatgtac tcctttccaa gtacgcgtca caggatgata tcattgtcgg ctcgccgaca
     5701 gctggcagaa cacatcctga tctgcaaggt gtgccgggta tgtttgtcaa cacggtggca
     5761 ctcagaacgg caccagcggg agataaaacc ttcgcgcaat ttcttgaaga ggtcaaaaca
     5821 gccagccttc aagcattcga gcaccagagc tatccgcttg aggagctgat tgaaaagctt
     5881 ccgcttacaa gggatacaag cagaagtccg ctgttcagcg tgatgttcaa catgcagaat
     5941 atggagattc cttcattaag attaggagat ttgaagattt cctcgtattc catgcttcat
     6001 catgttgcga aatttgatct ttccttggaa gcggtcgagc gtgaagagga tatcggccta
     6061 agctttgact atgcgactgc cttgtttaag gacgagacga tccgccgctg gagccgccac
     6121 tttgtcaata tcatcaaagc ggccgcggct aatccgaacg ttcggctgtc tgatgtagat
     6181 ctgctttcat ctgcagaaac ggctgctttg ctagaagaaa gacatatgac tcaaattacc
     6241 gaagcaacct ttgccgctct ttttgaaaaa caggcccagc aaacacctga ccatcctgcg
     6301 gtgaaggctg gcggaaatct gctgacctat cgcgagctcg atgaacaggc gaaccagctg
     6361 gcgcatcatc ttcgtgccca aggggccgga aatgaagaca ttgtcgcgat tgttatggac
     6421 cggtcagctg aagtcatggt atccattctc ggtgtcatga aggcgggggc agctttcctt
     6481 ccgattgatc ctgatacacc tgaagaacga atccgttatt cattagagga cagcggagca
     6541 aaaattgcgg tcgtgaatga aagaaacatg acggctattg ggcaatatga agggacaatc
     6601 gtcagccttg atgacggtaa atggagaaat gaaagcaagg agcgcccatc atccatttcc
     6661 gggtcttgca atcttgcata cgtcatttat acgtccggta cgaccggaaa gccgaaggga
     6721 gtgcagattg agcatcgtaa tctgacaaac tatgtctctt ggtttagtga agaggcgagc
     6781 ctgacggaaa atgataagac tgtattgctt tcatcctacg catttgacct tggctatacg
     6841 agcatgttcc ctgtacttct gggcgggggc gagctccata tcgtccagaa ggaaacctat
     6901 acggcgccgg atgaaatagc gcactatatc aaggagcatg ggatcacata tatcaagctg
     6961 acaccgtctc tgttccatac aatagtgaac accgccggtt ttgcaaaaga tgcgaacttt
     7021 gaatccttac gcttgattgt cttgggagga gaaaaaatca tcccgactga tgttctcgcc
     7081 ttccgtaaga tgtatggaca taccgaattt atcaatcact acggcccgac agaagcaacg
     7141 atcggcgcca tcgccgggcg ggttgatctg tctgagccgg atgcatttgc gaaacgcccg
     7201 gcaatcggac gcccgattgc gaatgccggc gcgcttgtct taaatgaagc attgaagctt
     7261 gtgccgccgg gagcgagcgg acagctctat atcacgggac aggggcttgc gagagggtat
     7321 ctcaacaggc ctcagctgac agccgagaga tttgtagaaa atccatattc gccgggaagc
     7381 ctcatgtaca aaaccggaga tgtcgtacga agactttctg acggtacgct tgcatttatc
     7441 ggccgggctg atgatcaggt gaaaatccga ggctaccgca ttgagccgaa agaaattgaa
     7501 acggtcatgc tcagcctcag cggaattcaa gaagcggttg tattagcggt ttccgagggc
     7561 ggtcttcaag agctttgcgc gtattatacg tcggatcaag ctattgaaaa agcagagctc
     7621 cggaagcagc tttccctaac actgccgtct catatgattc ctgctttctt tgtgcaggtt
     7681 gacgcgattc cgctgacggc aaacggaaaa accgacagaa acgctttgcc gaagccgaac
     7741 gcggcacaat caggaggcaa agccttggcc gcaccggaga cagcgctcga agaaagctta
     7801 tgccgcattt ggcagaaaac gcttggcata gaagccatcg gcattgatga caattttttc
     7861 gatttaggcg gccattcatt aaaagggatg atgctgattg ccaacattca ggcggcattg
     7921 gagaaaagcg taccgcttaa agcactgttc gagcagccga ctgttcgcca gctggcggct
     7981 tatatggagg cgtctgccgt ttcaggcggc catcaagtcc tcaaaccggc tgacaagcag
     8041 gctatgtatc cattgtcatc cgcccagaaa cggatgtacg tgctcaatca gcttgaccgc
     8101 cagacgataa gctacaacat gccatctgtt cttctaatgg aaggagagct tgatatttcg
     8161 cgcctgcgtt actcactcaa tcagcttgtg aaccgtcacg aatcattgcg gacatcattt
     8221 atggaagcta atggtgagcc tgttcagcgc atcattgaga aggcggaggt tgatcttcat
     8281 gtgtttgaag ccaaagaaga cgaagcggac caaaagatta aggaatttat ccggccattc
     8341 gacttaaacg acgcaccgct cattcgcgca gctttgcttc gaatagaagc aaaaaaacat
     8401 ttgctgcttt tagatatgca tcacatcatc gcagacggcg tctcaagagg catttttgta
     8461 aaagaattgg cgctgcttta caaaggagaa cagcttccgg agccggcgct tcattataaa
     8521 gatttcgccg tttggcaaaa tgaagctgag caaaaagaac ggatgaagga gcatgaggcg
     8581 tactggatgt cagttctttc aggcgagctg ccagagcttg atctcccgct cgattatgcc
     8641 cgtccgcctg tgcaaagctt taaaggagat acgatccgtt tccgtacggg aagtgagacg
     8701 gcaaaggcgg tagaaaaact gcttgcggaa accggaacga ccttgcacat ggtgctccat
     8761 gctgttttcc acgtcttttt aagcaaaatt tccggacagc gggatatcgt catcggctcc
     8821 gttactgccg gccggacgaa tgctgatgtt caggacatgc cgggaatgtt cgtcaataca
     8881 cttgccctga gaatggaagc gaaagaacag caaacatttg cggagctttt ggagcaagca
     8941 aagcagacga acctgtcagc gcttgagcat caggagtatc cgtttgaaga tctggttaat
     9001 cagcttgatc tccctcggga tatgagccga aacccattgt ttaatgtgat ggtgacgaca
     9061 gaaaaccctg ataaagaaca gcttacattg caaaatctga gcatttcacc ttatgaggct
     9121 catcagggaa cttctaagtt tgatctgaca ctgggcggat ttactgatga aaatggaatt
     9181 ggcttgcagc tcgaatatgc gacagatctg ttcgcgaaag aaacagttga aaaatggagc
     9241 gaatacgttc tgcggctact aaaggctgtt gcggataacc cgaaccagcc gctttccagc
     9301 ctgtcacttg tcacagagac agaaaagcaa gcacttcttg aggcatggaa gggcaaagcg
     9361 ctgcctgtgc cgacagacaa aacggttcat cagctattcg aagagactgt ccagcgccac
     9421 aaagacngnc cggctgtcac atacaacggc cagtcttgga cgtacggcga gctgaatgcg
     9481 aaggcaaacc gtctcgc
//
