LOCUS       NZ_VDTN01000001        21107 bp    DNA     linear   CON 13-OCT-2024
DEFINITION  Priestia megaterium strain DE0399
            NODE_1_length_2016715_cov_40.972596, whole genome shotgun sequence.
ACCESSION   NZ_VDTN01000001
VERSION     NZ_VDTN01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Priestia megaterium (Bacillus megaterium)
  ORGANISM  Priestia megaterium
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Priestia.
REFERENCE   1  (bases 1 to 2016715)
  AUTHORS   Zhang,C.
  TITLE     Temporal encoding of bacterial identity and traits in growth
            dynamics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2016715)
  AUTHORS   Song,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2019) BGI-CNGB, BGI, No. 11, Beishan IZ., Yantian
            Dist., Shenzhen, Guangdong 518083, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.11.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: BGISEQ
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007672925.1-RS_2024_10_12
            Annotation Date                   :: 10/12/2024 01:56:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,486
            CDSs (total)                      :: 5,426
            Genes (coding)                    :: 5,372
            CDSs (with protein)               :: 5,372
            Genes (RNA)                       :: 60
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 50
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 12 of 55
            Pseudo Genes (incomplete)         :: 40 of 55
            Pseudo Genes (internal stop)      :: 11 of 55
            Pseudo Genes (multiple problems)  :: 8 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:32:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 891531
            Orig. end                         :: 912638
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VDTN01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21107
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RRE-containing"
                     /region_number="2"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..21107
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21107
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11107](-)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11107)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     gene            105..830
                     /locus_tag="FS483_RS04470"
     CDS             105..830
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082644.1"
                     /locus_tag="FS483_RS04470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028413790.1"
                     /transl_table=11
                     /translation="MHIEINTLFTLGSVMGLLVGGGLLKLVSHLSRGRIQFMYAICGGI
                     LLGLLFFDILPSTWHLYDLKGVLLGIVAGYIVMLCIDLFLHKTNSAISPFQSLILLTAA
                     IFFHNIITGITFGITDLHQSASVFVATLLHQIPEGMALMTVLSIAKIPNVFFLFIIVLL
                     SLSLGGSLLLGESVQASSLKIQALSTGGAIGTLSFVIFHEIIGKAISHFRNLHVWLLIS
                     LGVLIIYIYHYSLSLFHSH"
     gene            1286..2221
                     /locus_tag="FS483_RS04475"
     CDS             1286..2221
                     /GO_function="GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810499.1"
                     /locus_tag="FS483_RS04475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_154989069.1"
                     /transl_table=11
                     /translation="MVDNHNHDHGDVFHSHAPIGKMKLAFFLTLIILVVELIGGFISRS
                     LALLSDAGHVLTDIAAIGLSWFAMHEAQKPASENFTYGYHRSGILAAFINAMTLIVIAV
                     VILYEAIDRFKDPQPVGSTWMFISASVGLVMNLYLGLGLRKEDNINVKSAVLHMIGDAM
                     ASAGVIIGGLIILFTGWNIVDPILSVLIAFLIALGAWKIVKQTIGILMEGTPKGISLEK
                     VMEAIHAVKGVLNVHDLHVWSITSGKNALSCHVVLNNQISFLEIQVILREIESKLQHQE
                     IEHATIQLENEEHPHDESAICSTTKREGHH"
     gene            complement(2559..2708)
                     /locus_tag="FS483_RS27015"
     CDS             complement(2559..2708)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FS483_RS27015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_155017187.1"
                     /transl_table=11
                     /translation="MELGYKKNIQLKDRDLKYKIKDSEETIELIVFIALLSVIFIINTF
                     FKFL"
     gene            3025..3234
                     /locus_tag="FS483_RS27950"
     CDS             3025..3234
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016763.5"
                     /locus_tag="FS483_RS27950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VanZ family protein"
                     /protein_id="WP_081732881.1"
                     /transl_table=11
                     /translation="MLDLSVTCLIEFIQYFMGRSLDIDDVLLNMFGTVISYVVLLINKQ
                     ASKIKKGDYIDRCPFCSNKLVDGN"
     gene            complement(3854..4357)
                     /locus_tag="FS483_RS04485"
     CDS             complement(3854..4357)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1250: DinB"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016764260.1"
                     /locus_tag="FS483_RS04485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DinB family protein"
                     /protein_id="WP_028413792.1"
                     /transl_table=11
                     /translation="MEKLEYEWVKQARQTLLEQCRELSEGDLTKEFAFGFQNIRDSLVH
                     VAGCYHAWLGSFVLSETSSPLFTKEVINTMKIDDIQHYFQQADRYVEKVLEQFTDKFDE
                     SIEKELAWRANSGFVRKTPRQLLTHSITHESHHKGQIVVMLRLLGHTPKNTDILGLPEK
                     EPFK"
     misc_feature    4413..4426
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 24.83"
                     /tool="antismash"
     gene            4642..5094
                     /locus_tag="FS483_RS04490"
     CDS             4642..5094
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414361.1"
                     /locus_tag="FS483_RS04490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /protein_id="WP_154989093.1"
                     /transl_table=11
                     /translation="MEKNNLLRKDNVSIVVESLDDAISFFKEIGLSLEGRATVEGQWGG
                     RVTGLGSQRVEIAMMVTPDGHSRIELSRFLSPPTISDHRNAPVNALGYLRVMFTVEDID
                     EMVSRLTRYGAQLVGEVVQYEDSYRLCYIRGVEGLLIGLAEQLSNK"
     gene            5299..5772
                     /locus_tag="FS483_RS04495"
     CDS             5299..5772
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016766408.1"
                     /locus_tag="FS483_RS04495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028413794.1"
                     /transl_table=11
                     /translation="MRRAKQAVVASAVGLAMLATPLALPNLGGSAGSAEAAEVGGDVAI
                     DPVAIGQAIADAAKTADNRSGFVKGAMEKAFFESGQQYNVIVMNLSQGYNSDQLQGVQY
                     FDTVDYDGITFGVWIFEEGTFINEGDGGYDNWAFRGWFERTGDDEKTVNFHRP"
     gene            complement(6124..7161)
                     /gene="recA"
                     /locus_tag="FS483_RS04500"
     CDS             complement(6124..7161)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /gene="recA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082635.1"
                     /locus_tag="FS483_RS04500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="recombinase RecA"
                     /protein_id="WP_028413795.1"
                     /transl_table=11
                     /translation="MNDKQAALDMALKQIEKQFGTGSIIKMGEGTHTKVEASSSGSLAL
                     DIALGINGYPKGRIMEVYGPESSGKTTVSLHAIAEVQKNGGRAAFIDAEHALDPVYARK
                     LGVDIDELLLSQPDTGEQALEIAEALVRSGAVDIIVVDSVAALVPQKEIEGEMGDSHVG
                     LQARLMSQALRKLSGAINKSKTIAIFINQIREKVGVMFGNPETTPGGRALKFYSSVRLE
                     VRRAEQLKLGNDIVGNKTRIKVVKNKMAPPFRIAEVDIMYGEGISREGEILDIGSEVDI
                     VQKSGSWFSYNGERLGQGRENAKVFLKENPEIREEIRKNIIDFKYGNTDNDNDIDNDSD
                     SENES"
     misc_feature    complement(7324..7338)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.02"
                     /tool="antismash"
     misc_feature    7325..7338
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 22.97"
                     /tool="antismash"
     gene            7674..8024
                     /locus_tag="FS483_RS04505"
     CDS             7674..8024
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002161992.1"
                     /locus_tag="FS483_RS04505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ASCH domain-containing protein"
                     /protein_id="WP_028413796.1"
                     /transl_table=11
                     /translation="MSNHEAQNELPPKTCAIDRLVTMPDDVNKVIKGSKTATRRNGRYA
                     DVGEIMVLEDQKFVVERVYSQSLGELTDENARQEGYATVEEYKQSILSYHPGMPWLPQM
                     RVWVHEFRLVND"
     gene            8043..8477
                     /locus_tag="FS483_RS04510"
     CDS             8043..8477
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002100522.1"
                     /locus_tag="FS483_RS04510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2269 family protein"
                     /protein_id="WP_028413797.1"
                     /transl_table=11
                     /translation="MLYRILVYIHVSSVILSIGPFFILLPIVKKLHSSREQEMNAYLIT
                     FQSVVRLAKHTGHVLVITGILLVMCGPWTWTTPWIIMTLIFLFCSLFFLARAFSPTLRK
                     YKEEGQNKKELATKLERSIWIYIILLMTMLWFMVGKPTFW"
     gene            complement(8745..9980)
                     /locus_tag="FS483_RS04515"
     CDS             complement(8745..9980)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937089.1"
                     /locus_tag="FS483_RS04515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_372454547.1"
                     /transl_table=11
                     /translation="MKSYEVLKNRDFLCLFLSATTSKLGTSFFQIALPLLVYDLTKSPS
                     LMALTFVLEITPQVLFSLLGGALADRISKKYILLIGDILSAILIIIIPLAASINVLDIW
                     IVYVVAFLISAVSAFYHPSFESVIPEILEGENLIQGNSLFKLSETITTFAGPSIAGILI
                     ALVGTHNLLYFNFFTYFLSGIFIGLITKKYKSQNPVNSSIMNSIAEGVKYVINTKKILI
                     GTILIFLINVGYGAMEALFMFHLKDNLHLSAQYIGLIFSLQTLGSFVAIYAANKLNKLP
                     RGNIIIYCGVIIGLGQTILTFSNTAVILLIVCRIVIVGSVTLLAINWFTLRQEIVPSAL
                     LGRVISSTRMLAFLALPISGMLAGIAAEYISVSLIFISAGILTIVASLIGIKTSLFETQ
                     QSIDQPINSSNK"
     gene            complement(10001..11107)
                     /locus_tag="FS483_RS04520"
     CDS             complement(10001..11107)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Pantocin_Microcin_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ThiF"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802791.1"
                     /locus_tag="FS483_RS04520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ThiF family adenylyltransferase"
                     /protein_id="WP_098113348.1"
                     /sec_met_domain="Pantocin_Microcin_RRE (E-value: 3.8e-17,
                     bitscore: 59.1, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="ThiF (E-value: 7.2e-43, bitscore: 143.6,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPKFKETIPVFFINDEIHIGEEDGVAGVIEDPSGSIKYLISLIN
                     GKNSIEEIIHLVQEKYPNIKENEVLEGIKALDTEKYLEDNSLNPQSIGDYELERYKANL
                     NYFSLFTSLEDNKYNVQETIINTKVALLGIGGLGSQILYHLSALGFHNINALDFDTLEL
                     SNFNRQLLYSEKDINKLKTELAKERISRFNPNVNLNVTNKRIECAEDVMAHIEGSDYVI
                     CVADKPTLHIQDWVNEAVVKLGKPLVSGGVLNTRGRFYSMLPGKTGCVECHKQTIIESD
                     VIVNKQLDSMHQINFQRNNAAISPNVAMLAGAIVNEFLGLVTGIQTPTSLGKMMELNFL
                     TYQTYPISEWNKKEDCPVCHSAPVTAGK"
     aSDomain        complement(10847..11107)
                     /aSDomain="Pantocin_Microcin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing E1-like protein in a
                     pantocin/microcin cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_FS483_RS04520_Pantocin_Microcin_RRE.1
                     "
                     /evalue="5.40E-19"
                     /identifier="RREFam017.1"
                     /label="FS483_RS04520"
                     /locus_tag="FS483_RS04520"
                     /protein_end="87"
                     /protein_start="0"
                     /score="59.1"
                     /tool="antismash"
                     /translation="MKPKFKETIPVFFINDEIHIGEEDGVAGVIEDPSGSIKYLISLIN
                     GKNSIEEIIHLVQEKYPNIKENEVLEGIKALDTEKYLEDNSL"
     gene            complement(11440..12351)
                     /locus_tag="FS483_RS04525"
     CDS             complement(11440..12351)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012933.5"
                     /locus_tag="FS483_RS04525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="WP_028413800.1"
                     /transl_table=11
                     /translation="MRQKIIRINKPSIYNFDLILRYIKNSPSFVIEKVIENTYCRAFII
                     GGRTYLVLVRDEGIDSKYLEVKVIGEEVKNNQVQLVKEKIITIFCLEQDINKAQKSIAN
                     ESKIGKLIKMYEGFRPVLLGSVYESIIWAIIGQQISAKVAHRIKKSLLEKTGAKIIINN
                     CEYYTDLCPHKVMKLNINQLRDLKLSQRKAEYIHEITKAIIDEELDLSCLYLLDREGGC
                     SYLMKHRGIGKWTAESILMRGIGRQDILPAGDLIIRKVVGEIYEYNELLTESQVRKIST
                     QWKTAETWITHLCWFYMQEKIL"
     gene            12512..12988
                     /locus_tag="FS483_RS04530"
     CDS             12512..12988
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014471322.1"
                     /locus_tag="FS483_RS04530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DinB family protein"
                     /protein_id="WP_154989095.1"
                     /transl_table=11
                     /translation="MDAMSFILLNFEEVRRRSIKVWMSIPQDRLDWRPDPEAMSCREMI
                     HHVLEGEYLYHQMLEKPLNLSLTEKHNPYKAVTFSSVEEALKFAQPYREKFIEFLGLLK
                     EKQLTEIKIDRSEMGYIRELGDMLLRVAYHESVHTGQLLDYLRTAKVKRPIIWD"
     gene            13085..13930
                     /locus_tag="FS483_RS04535"
     CDS             13085..13930
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231: carboxyvinyl-carboxyphosphonate"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009333858.1"
                     /locus_tag="FS483_RS04535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="WP_028413802.1"
                     /sec_met_domain="PEP_mutase (E-value: 4.4e-76, bitscore:
                     252.4, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNEKQLLKFQTFQQLHKRPYTFVLPNAWDVISAKIFEKNGFPAI
                     ATTSAGIAMSLGYNDGEKIPFEQMLGAVKRIINSVNIPVSVDLESGYGRSTNEVVDNTR
                     QIILAGGIGINLEDGTGDLDSPILDVSLQVEKISEIRLLSKSLKAPLFINARTDLYWLN
                     TGDPDLRLQEAINRGKAYQKAGADCIFIPGLYDMESIKKVRQELSCPINLLSGPNLPSI
                     NTLSQVGIERISTGSAPFRATATLLQRITEDILYDNNFQRLMNDNMSYDAITELIGTPE
                     "
     misc_feature    complement(13975..13993)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.05"
                     /tool="antismash"
     misc_feature    14241..14254
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.97"
                     /tool="antismash"
     gene            14358..15212
                     /locus_tag="FS483_RS04540"
     CDS             14358..15212
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056499.1"
                     /locus_tag="FS483_RS04540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_028413803.1"
                     /transl_table=11
                     /translation="MNLIDKKVTHKHFGTGTIVKYNDSSIEINFASENKKFVFPDVFGK
                     HLKLHDKSVAHSLEKILEEKETERKEEEWKKEEEKKLQRKNQELRWGHEKLMKNHKLHP
                     ESQMVFWCDTEEQNSSFSEWTVFSGVIKSGNYKGKPTKPTRLHQNSAVLLTAIDSSMPE
                     KDRRILGVYMVNENFIGKLCEDGYIPAHSTYRLQLTEQESNQMLFWKYYINEKNSQKMV
                     WNTGKYRYFDNSWMAQILLDIVSLKSDSNEREQAQQFFEHFCKMNLITDQDLPKPNGAL
                     MRI"
     gene            15951..16184
                     /locus_tag="FS483_RS04545"
     CDS             15951..16184
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FS483_RS04545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_028413804.1"
                     /transl_table=11
                     /translation="MSNYEKTKNNKNIILGIIYALIVISVYAFNLHLQIALSILTLVII
                     FELVTSIKEDDKAKNVINIITICLFILGIVFL"
     gene            complement(16531..18294)
                     /gene="dhaK"
                     /locus_tag="FS483_RS04550"
     CDS             complement(16531..18294)
                     /EC_number="2.7.1.121"
                     /GO_function="GO:0004371 - glycerone kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="dhaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056511.1"
                     /locus_tag="FS483_RS04550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="WP_154989097.1"
                     /transl_table=11
                     /translation="MKKIINKPENVVIEMCKGMVLAHPELNLIEKYKVIKKEQINKNKV
                     SIISGGGSGHEPAHAGYVGKGMLDAAVCGDVFASPSQIQIYQAIKETASDKGTLLVIKN
                     YSGDMMNFKNAAYLAEEDGIQVDYVKVDDDIAVQDSLYTVGRRGVAGTVLVHKIAGAAA
                     ERGYDLPKVKEAAESAIANVKSIGIGLTSCTVPAKGTPTFEIAEDEMEFGVGIHGEPGI
                     RREKIISADELAERMVTALLKEIGIEDGKGEVAVLINGFGSTPLQELYLLNHSVIRELS
                     RRKVTIARTFVGNYMTAIDMAGASISIMKLDENLKSLLSEECDTPALKLKGEVPAVTYD
                     EIIGTAEAPKVSYEVQTNKEYSVVTENRLTLNNIIFMVDQMSECIIRNEIPFCELDSHA
                     GDGDFGMSVAKGFKQLKAEWHEILENKSNDIGNFLEACSIVIMEHCGGASGPIWGSAFR
                     AAGKNTESKTELTLTEFAEMIQASVKGIQATGERSFGRGAVVGDKTLIDALVPYADTLT
                     SSAAEGESLKHALVKAAEAAVEGAKSTEQIVARMGRAGTVGERSLGYPDAGAHGLGVIF
                     TEVAQVMQEKQ"
     gene            complement(18296..19429)
                     /locus_tag="FS483_RS04555"
     CDS             complement(18296..19429)
                     /EC_number="1.1.1.6"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1181: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460629.1"
                     /locus_tag="FS483_RS04555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glycerol dehydrogenase"
                     /protein_id="WP_028413806.1"
                     /sec_met_domain="Fe-ADH (E-value: 6.7e-63, bitscore: 209.6,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKAFISPSKYIQGEDEILNLGYFVKTFGTSALLIAHPEDIKRVQ
                     GKLDATAEKYGITFFEGGFNGECSRPEISRLQEIAKENNCDCTIGLGGGKAIDTAKCVA
                     EGEGLIIVPTIAATDAPTSHSAVIYTPEGAFDDYAYFKQSPSVVLIDTTVIANAPTRFL
                     VSGMGDALSTYFEARATARSFSNVNAGLPCGVREDVCAPAKGTNAALVLAEHCYNTLLE
                     DGVKAKAASDHNVVTPALENIIEANILLSGLGFESGGLAGAHAIHDGLTLLESAHHYFH
                     GEKVAFGTLAQLVLENAPTEEIEEVLDFCLAVGLPVCLADIGVEQITQEELMEVANKAC
                     IPEESIYSMPFPVTPESVAAAILAADQIGNDYKKRLI"
     gene            19741..20784
                     /locus_tag="FS483_RS04560"
     CDS             19741..20784
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013056509.1"
                     /locus_tag="FS483_RS04560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="WP_154989099.1"
                     /transl_table=11
                     /translation="MKTTYMNLDKILDLKKWRNLQDSLALVTKLAIVTVNYKGVPISQH
                     SGVRPFCETMRKNPNTAKLCEKCDARGGLEAVRTNAPYIYLCHWNIVDIAIPIIIDDKY
                     VGAIMAGEVKIEDGDKHPPLEQMLTVPSKGFEQKLNNNEEIQRLYDSIPTLSYTEINDA
                     AQMLYRLCNYIVEEAMNKNLILEVYEKMAPVKEDLSTEDISYGYSLDNIQNIKKELANT
                     VTNAYVKTSINDHLVCKNKVLQPAFDYIYKHKREKISQQKMADLCHISTGYFSRVFTKE
                     TGMNFSVFISLKKIEWSKQLLEKTDLSIAQISDELGFNDPGYYIKTFKKYENITPNVYR
                     KYYQEKM"
ORIGIN
        1 atgtactttt atttttttaa ccatttataa aacataggta tgattgccct tcctttgaat
       61 aggttagttg taaaaaaaca tatcgccgaa aggaagtagc aattatgcat atagaaataa
      121 ataccttatt tactttaggt tctgtaatgg gtttattagt aggtggagga cttcttaaat
      181 tagttagtca cctgtcacga ggtagaattc agtttatgta tgcgatatgt ggaggaatct
      241 tattagggtt gttatttttt gatatccttc cttccacatg gcatctgtat gatttaaaag
      301 gcgttctatt aggcatcgta gcaggatata tcgttatgct atgcattgac ctctttttgc
      361 ataaaacaaa tagtgcaatt tcaccttttc agtcacttat tttattaacg gctgcaatct
      421 tttttcataa tattattaca ggaattacct ttgggataac agatctacat cagtcagctt
      481 ccgtattcgt agctaccctc ttacaccaaa ttccagaggg tatggcccta atgaccgttc
      541 tctccatagc aaaaatacct aacgtcttct ttttattcat tatagtcctt ctatcactat
      601 ctttgggagg gagtttatta cttggagaaa gtgtacaagc ttcctcgctt aaaatacaag
      661 ctttatcgac cggaggagca attggtaccc tatcttttgt tatttttcat gaaataatag
      721 gaaaggctat tagtcacttc agaaacctac atgtatggct attaatatcg ctaggagtac
      781 taattattta tatttatcac tattcccttt cactatttca tagtcattaa acttatatag
      841 aaaattttag agatcttaat ttataaggtc tctttttcat tcttataaga ttaatcatat
      901 tacataagaa attcaaaagt cgtgatccga attttttctg atttgttaaa aagaagtatg
      961 agattgattt ggaaacagct tttgagtgac ataaatgatt tttaataaag ttgaaaatac
     1021 taaaataaat ggtcaaatta aatgtcgaaa taatgaaact ccttttattg ttttccagaa
     1081 cttaaaagca ctcactatct caccacaaaa gaattaacat tctatttcta ctgtcttaaa
     1141 aggaatgttt agtatatgga attttcaaca atttaaagat ccgccaattg agttttttaa
     1201 ataaactact tttaatcaaa tccaaaacaa gagaaataac gttttttaaa caatgatcag
     1261 ttttttgcag aaaggagaga ttcttatggt agacaaccat aatcacgacc acggagatgt
     1321 atttcactcc catgctccga ttgggaaaat gaaattagca ttttttctta ctttaattat
     1381 cctggtagtc gaattaattg gaggattcat ttcacgcagc ttagccctac tttctgatgc
     1441 tggacatgta cttacagata ttgcagccat agggctatcc tggtttgcta tgcacgaagc
     1501 ccaaaagcca gcttcggaaa atttcactta cgggtatcac cgttcaggga tactagctgc
     1561 ttttattaat gccatgacgt taattgttat cgctgttgtc atactatacg aagcgattga
     1621 tcgctttaag gatccccaac cagtgggaag tacgtggatg tttattagtg catctgttgg
     1681 tttagtcatg aacctttact taggtcttgg cttaaggaag gaagacaaca tcaatgtgaa
     1741 aagcgccgta cttcatatga ttggggacgc aatggcttcg gcaggagtta ttatcggagg
     1801 acttattatc ctattcactg ggtggaatat cgtagatcca atcctaagcg ttcttattgc
     1861 attcttaatt gctttgggcg catggaaaat tgtaaagcag acaataggaa ttttaatgga
     1921 aggtacaccg aaaggaatct ctttagaaaa agtaatggaa gcaattcatg cagttaaagg
     1981 tgtactaaat gtccacgatt tacatgtttg gagtataact agtgggaaaa atgcattatc
     2041 ctgtcatgtg gtgttaaata atcagataag tttcctggaa attcaggtca tattaagaga
     2101 aattgaatcc aaacttcaac atcaagaaat tgagcatgca actatccagt tagaaaacga
     2161 agagcaccct catgatgagt cagctatttg ctctaccacc aaacgtgaag gtcatcacta
     2221 atacaacttg gtgtttttat atgtgtaaac cctgaagaat attgctgtcg ataataaact
     2281 aaatgtatag gtcaacagac aaaaaaatca tgactcactt ttacgaatta ttatttccca
     2341 gctctttctt acattatgaa tgagtttttg tgtttttaaa aagatatatt ttcacgaaca
     2401 aaatattcaa atctattgca gagtgttaaa gaaattatta tcgttaataa aataaaaagc
     2461 catctagtat ttgaagtaaa ggtggctttt tactataata tgcacgcacg tccagacata
     2521 catactatat cttttcatac aatcacatgt aaaaaagact ataagaattt aaagaaagta
     2581 ttaataataa aaatcacact taaaagtgca ataaaaacaa tcaattcaat tgtttcctca
     2641 ctatctttta ttttatactt taaatctcta tctttaagct gtatgttttt cttatatcct
     2701 aattccatat gattaattcc ttccctttat gtattcgttt taattggcat gtttactatt
     2761 tggcttgttt ccacatgtaa gttagcaccc tctatactgt gttcttatgc taatgtattc
     2821 tctactttag ctgttatatc atgagcagcg tctactccca agatggaatt tgcgagaatg
     2881 gagatatcta ctacaggact ccataatgtt tcctctcttt tatcctaaca aagcaagttc
     2941 aagtgggtct atagcaacct ttttgaagga ttctataaaa ataaacccct atcaagaaag
     3001 tttcgttaag taaaaaaagt aacaatctta gatttatctg ttacatgttt aattgaattt
     3061 attcaatatt tcatgggacg tagcttagat atagacgatg ttctacttaa tatgtttgga
     3121 acagttataa gttatgtggt tcttttaata aataagcaag ccagtaaaat aaaaaagggc
     3181 gactatatag atagatgccc tttttgttcc aataagctgg ttgatggtaa ctaagtatat
     3241 aatttataaa acagaacaac ctcctgtaca ggaggttgtt ctgttgggaa tacgttatta
     3301 gtctggccag atttgtagat tcatatacat ataactttaa aaataaacat tgtgatgact
     3361 ctgtttttaa aaagcaacct tacttaagtt tttttagtta agtaattgat aaagtatttt
     3421 atttattatt tattttttac tatttacact tagtgacgac aaaatagttc tttttctaca
     3481 aattttccca acttgatatt actaaatacc actgaattta acgctcattc cgttatctag
     3541 atgaaataag aagatacctt ttcctcagag tccattagaa gaaatatttt tttcattaaa
     3601 accctccaac ttatatttat ggatttattt ctctgaaaag acaacaacaa taatttagtg
     3661 gatagcgtaa caacgatggt tggttaaatt tttgtgattc tagctgtgaa cgtcttcagc
     3721 aacacgttca ccaatatcca gcacacgcga gatgttttgc acaatttcag gaagcccgat
     3781 cacggcctct tggaacatcc aaatataaga taggactgca aatatgctct tcgtgaaatg
     3841 atttcttaat ttattatttg aaaggttctt tttcaggtaa tcccaatatg tccgtatttt
     3901 taggtgtatg tcctaataga cgtaacataa ctacaatttg tcctttatga tgtgattcgt
     3961 gagtaattga atgggtaagc aattgacgag gtgtctttct tacaaaacca ctgtttgctc
     4021 tccaagcaag ttctttttcg atagactcat caaatttatc tgtgaattgc tccaatactt
     4081 tttctacata tctatcagcc tgttgaaaat aatgctgaat atcgtctatt ttcatagtat
     4141 taattacttc ttttgtgaat agtggtgatg atgtttctga aagcacaaaa gaacctaacc
     4201 aagcatggta gcaacctgcc acatgaacta gagaatctct aatattttga aaaccaaatg
     4261 caaactcttt cgtcaagtca ccttcactca attctctaca ctgctctaat aaagtttgtc
     4321 gtgcttgttt tacccattca tattctagct tttccataac aatcccacct ctcagacata
     4381 taatgaaaaa ttattcttta ctatacactc tttagaatta ttattacacc cattttttca
     4441 ttaattagtt aagtaaatga taaagtactt cgtgtattaa tgacaaatag atcatttttc
     4501 tacaaaaaat ataaagtata caacaaatat ggcctagtga caggatagga atgttttgtt
     4561 tgaaaggaat ttgtaacttt taaatagaat gattatgaaa atggacagat aataaaattt
     4621 tacacataag gagataaaac tatggaaaaa aacaacctac taagaaagga caatgtcagc
     4681 atcgttgtag aatcccttga tgacgcaatt tctttcttta aggagattgg cttgagtctc
     4741 gaagggcggg ccaccgtcga aggtcaatgg ggtggccgcg taacgggatt gggttctcag
     4801 cgtgtagaga ttgcgatgat ggtcaccccg gatggtcaca gtcggattga actttcgcga
     4861 tttctcagcc cacctactat atcagatcac cggaatgctc ctgtaaacgc tctcggctat
     4921 ctacgcgtca tgttcactgt tgaagacatt gacgaaatgg tgtccagact tactagatat
     4981 ggcgctcagc tcgttggcga agtggttcag tacgaggact cgtatcgtct ctgctacatt
     5041 cgtggagtag aaggactttt aatcggtttg gcagagcaac tcagtaacaa ataagtaagt
     5101 gacgttttat aaaagtcttt actgaaatgt acgtttaacc tatctgaatc ttcaatagat
     5161 cgaggttatt ttcaggtttt ttcaaacctt tattttactt tttcgccccc ctatatatac
     5221 ttaattatgg aatttttgtt ttaatattca taaaaagggt attagttcta agaatcaact
     5281 aaaataggag gagatcaaat gagaagagcg aaacaagctg ttgtagcttc tgctgtaggt
     5341 ttagctatgt tagcgacgcc attagctcta ccgaatttag gaggttctgc tggctccgca
     5401 gaagctgcag aagttggagg agacgtagcg attgatccag tagccatagg acaagcgatt
     5461 gcagatgcgg ctaaaacggc cgacaatcga agtggttttg ttaagggagc gatggaaaaa
     5521 gctttctttg aatcgggtca gcagtataat gtgatagtaa tgaatttaag tcaaggttat
     5581 aattccgacc agttacaagg tgtacaatat tttgataccg tagattatga tggtatcacg
     5641 ttcggggtct ggatatttga agaaggaacg tttattaatg aaggtgatgg aggttatgat
     5701 aactgggcct ttagaggttg gtttgaacgc acgggggacg atgagaaaac ggtcaacttc
     5761 catagacctt gatctaatta aacagaaccc tatttaatta aatagggttc tgtttgtttg
     5821 ctataagaat ttttctgttt aaagagattg caataaaaag tctagtatag cgctttagac
     5881 aacagtatct caattagaaa tcttgttcct tccacagtag cccaattttt tgttttctct
     5941 gcaaaacctt ttctagataa cgttccgtta ttttacgatc actgtggcca agctcctttt
     6001 gaattaagac aatatcagct ccctgctggc tactataaat cgcataaaat gaccaaaata
     6061 atgaagtgaa atcagtgaaa tggatgggct tgaagctgcc gggaaaggca ttttaagctt
     6121 ttattagctt tcgttttcac tatcgctatc gttatcaata tcattatcgt tgtcagtatt
     6181 accatattta aaatcaataa tgttttttct aatttcttct ctgatttcag ggttttcttt
     6241 taagaaaacc ttagcgtttt ctcgtccctg ccctaaacgt tccccattat aagaaaacca
     6301 agatccagac ttttgaacaa tatctacttc agaaccaata tctaagattt ctccttctct
     6361 tgagatacct tctccataca taatatctac ttcagcaatg cggaaaggag gtgccatctt
     6421 atttttgact actttaattc ttgttttgtt accaactatg tcatttccca gtttaagttg
     6481 ttcagcgcgg cgaacctcaa ggcgaacgga agaatagaat tttagcgctc ttccgcctgg
     6541 cgttgtttca ggatttccaa acataacgcc taccttctcc cggatttggt tgatgaagat
     6601 agcaatggtt ttagacttat taattgctcc agatagttta cgaagggctt gagacatcaa
     6661 acgagcttgt agacctacgt gagaatctcc catttctccc tcaatttctt tttgaggaac
     6721 taatgctgct actgagtcaa caacaataat atctacagca ccacttctaa ctaatgcttc
     6781 agcaatttca agtgcctgct caccagtatc tggttgagat aacagtaact catcaatatc
     6841 tacacctagt tttcttgcgt aaactggatc aagtgcgtgt tcagcatcaa taaaggcagc
     6901 tcgacctcca tttttttgta cttctgcaat cgcatgtaag gaaactgttg ttttacctga
     6961 gctttcgggt ccgtacactt ccatgattct tccttttgga taaccattaa tcccaagagc
     7021 gatatctaaa gctaatgaac ctgagctaga tgcctcaact tttgtatgtg tgccttcccc
     7081 catcttaata attgatcctg taccgaattg tttttcaatt tgttttagag ccatatctag
     7141 tgctgcttgt ttatcattca taatttaaaa cctccgattg ttagtacgtt tacagtaaac
     7201 gacaatgtat gtggataaat aataaggatt ggctcaaatg tttttgctcc gtgcgagaaa
     7261 attttgtgtt taactgtctt ggtttattct gttgttcctt tatttatctc taaaccctta
     7321 atatcgaaca aatgttcctg ttgtgtaatc atacaataaa cgcatctagt tgtaaaggag
     7381 aaagttttgt acaaaccagc caaagaaata tcttttttgt aaaaagtagg tttatacctt
     7441 taatacttac agttattgaa acttcaatct ataaaataac ccttattata cgaacaaaga
     7501 ataattcatt ctcttatata cattatgtta tgtacttgga ctatcgaata agaaaaggga
     7561 taaattataa tagatttatt agagaaaatt tccacctaat aaattcaaat gaaattcgtc
     7621 gttaaaaata tgatacatta aatacgagac aagatatgaa aggagttttt ttaatgagta
     7681 accatgaagc acaaaatgaa ttgccaccaa aaacatgtgc aatcgatcga cttgtaacaa
     7741 tgccggatga tgtaaataaa gtaatcaaag gcagcaaaac agcaactcgc cgaaatggaa
     7801 gatatgctga tgtaggagag ataatggttt tagaagatca aaaatttgta gtggaacgag
     7861 tttattctca atcactaggt gaattaaccg atgaaaatgc tcgccaggaa ggctatgcta
     7921 cagtagagga atataaacaa tctattttgt cttatcatcc tgggatgcct tggcttccgc
     7981 aaatgagggt atgggtgcat gagttcagat tagtcaacga ttgataggta aaagaaatgg
     8041 atttgttgta ccgtatatta gtatatattc atgtttctag tgtcatttta tcgattggtc
     8101 ctttctttat tctcttgcca attgttaaaa aattacatag ttcaagagaa caagaaatga
     8161 atgcctattt gatcacattt caatctgttg ttaggctcgc aaaacatacg ggccatgttc
     8221 ttgttatcac agggatactc ctcgttatgt gtggcccatg gacgtggact acgccgtgga
     8281 ttataatgac actgattttc cttttttgct ccttgttctt tctggcccgt gctttttcac
     8341 caacacttcg aaagtacaag gaggaaggac aaaataaaaa agaactggca actaaactag
     8401 agcgatctat ttggatttat atcattctat taatgactat gctttggttt atggtcggta
     8461 agccaacttt ttggtgacac ccatccattt gtcatcacta agagtaaata gttaaactga
     8521 ttattttcat caaatacttt acgttttact taacataact cgtattttag gtatgaaaaa
     8581 agatttttgc ttgtatatca gatgcacatt tggattaaaa ataaaaaacc cctattaacc
     8641 atatgttaaa ggggttctta cttattgttt aaactccaag tatgaagttc ttatagaaga
     8701 atacactaaa tatcaatatg tttcaagtca acccgagtaa ttatctactt gttgctacta
     8761 ttaataggtt gatctataga ttgttgggtc tcaaatagag aggtcttaat gccgattaaa
     8821 gacgctacaa tcgttaggat gcctgctgaa ataaaaatta aagagacgct aatatattca
     8881 gctgcaatac ctgctaacat tccactaatc ggtaaagcca aaaaagctaa catacgagtt
     8941 gaactaatta ctcttcccaa taaggctgat ggcacaatct cttgcctaag ggtaaaccaa
     9001 ttaattgcta ataatgttac cgaaccgaca attacaattc tacagactat taacaaaata
     9061 actgcagtgt ttgaaaaagt aaggatagtt tgccctaaac ctattattac accacaatag
     9121 ataattatat ttccccgtgg taatttattt agtttatttg cagcataaat tgctacaaat
     9181 gaccctaagg tttgtaggga aaatattaaa ccaatatact gtgccgataa atgtaaatta
     9241 tcttttagat gaaacataaa taaagcttcc atagcaccat aacccacatt gattaagaat
     9301 atgagaatag tccctatcaa aatcttttta gtatttataa cgtatttaac tccttcagct
     9361 attgaattca ttatagaact attaactgga ttctggcttt tatatttctt agttattaag
     9421 ccaatgaata ttccagaaag aaaatacgta aaaaaattaa aatataataa gttatgagtt
     9481 ccaaccaaag caattaaaat gcctgctatt gaagggcctg caaaggttgt aattgtctct
     9541 gataacttga ataaagagtt accctgtatt aaattttcac cttctaaaat ttctggtatg
     9601 actgattcaa aacttggatg ataaaatgca ctcacagctg atattaaaaa agctaccaca
     9661 tatacaatcc atatatctaa cacgtttatt gaagctgcaa gaggtatgat aataattaaa
     9721 atggctgata aaatatcacc tattaataaa atgtatttct tacttatacg atcagctaag
     9781 gctccgccta acaaactaaa caatacttga ggcgtaattt ctaaaacaaa tgttaatgcc
     9841 attaaacttg gtgatttagt caaatcatat actaataaag gcagtgcgat ttgaaaaaaa
     9901 gacgtgccca acttacttgt tgtggcactt aaaaataagc ataaaaaatc tctgttcttt
     9961 agtacttcat aagatttcaa catttacccc cccatcataa ttatttacct gctgttacag
    10021 gtgcggaatg gcaaactgga caatcctctt tcttattcca ctctgaaata gggtaagttt
    10081 gatatgtaag gaaatttaat tccatcattt tccctaatga tgttggagtt tgaatacctg
    10141 ttaccaaccc taaaaattca tttacaatag caccagccaa catagctaca ttaggactaa
    10201 tcgcagcatt atttcgttga aaatttatct gatgcatact gtcaagttgc ttattaacta
    10261 taacatcact ttcaattatg gtttgtttat gacactctac acacccagtt tttcctggta
    10321 acatagaata aaatctgccc cttgtgttta ggactccacc tgaaactaac ggctttccta
    10381 acttcactac tgcttcattt acccagtcct gtatatgaag ggttggttta tcggctacac
    10441 aaattacata atcactacct tcaatatgtg ccattacatc ttcagcacat tctatacgct
    10501 tattagtaac gtttaaatta acattaggat taaaacgcga tattctttcc tttgctaatt
    10561 ccgtttttaa cttattaata tctttttcag aatataacaa ctgtctatta aaattgctta
    10621 attccaaagt atcaaaatcc aatgcattaa tgttatgaaa acctaatgca gatagatgat
    10681 atagtatttg actacctaaa cctccgattc cgagcaaagc aacctttgta tttatgatag
    10741 tttcttgcac attgtactta ttgtcctcta aagacgtaaa aagcgaaaag taattaaggt
    10801 ttgctttgta tctttcgagt tcataatctc ctatagattg aggattcaaa gaattatctt
    10861 ctaaatattt ctctgtatct aatgccttta tcccttccaa tacctcattt tcttttatgt
    10921 ttggatactt ttcttggact aaatgtatta tttcttctat agaatttttt ccgttaataa
    10981 gagaaattaa atacttaata cttccactgg gatcttcaat aactcctgct actccatctt
    11041 cttcaccaat atggatttca tcatttataa aaaatacagg gattgtctct ttgaacttag
    11101 gtttcatttt ctacatttca ctccatttct cttaattatt gaaataggga actatcatat
    11161 gatagttccc tatatttaca gacttgaagt ttaaacaata agtaagtagt tcacataatg
    11221 ataacgtttt agcctggaat accgtttaat accgcagctt tattagttgt tttcttaact
    11281 tttaccttca taactaacac ctccatttct taaaagatat atttataata ttgcattaaa
    11341 aaggcatttt caacaattaa atccaatttt tataatattc aaacaaaaac aggtaaatta
    11401 tacttttttt cgtttatttt aaaccgacaa atattccatt tataatattt tttcttgcat
    11461 gtaaaaccag catagatgag taatccaggt ttccgcagtt ttccattgag tagatatttt
    11521 tctaacttgt gattctgtta ataattcgtt atactcataa atctctccta ctactttacg
    11581 tataattaaa tctcctgcgg gtagaatgtc ttgacgtcct atacctctca ttagtattga
    11641 ctctgccgtc cattttccta tacctctatg tttcattaaa tagctacaac ccccttcacg
    11701 atctaataaa tataagcaac ttaagtctag ctcctcatct ataatggctt tagttatttc
    11761 atgaatatat tcagctttcc tttgacttag ctttaagtca cgaagttggt ttatatttaa
    11821 cttcatcacc ttgtgggggc ataaatcggt ataatattca cagttattaa ttattatttt
    11881 agctcctgtt ttttctaaca aactcttttt aatcctatga gcaacttttg cactaatttg
    11941 ttgaccgatg attgcccata tgatggattc gtagacactt cctaataaaa caggtctaaa
    12001 cccttcgtac atttttatta attttcctat cttagattca ttagctatag acttttgagc
    12061 tttgtttata tcttgttcca gacaaaatat tgtgataatt ttctccttta ctagttgaac
    12121 ctgattattc ttaacttctt caccaataac tttaacttcc aagtacttac tatcgattcc
    12181 ttcgtcccta actagtacta ggtacgttct tccccctatt ataaaagctc tacaatatgt
    12241 gttttcaata accttctcga ttacaaaaga gggcgagttt tttatgtacc ttaaaattaa
    12301 atcaaaatta tatatgcttg gtttattaat tctaattatt ttttgtctca taacttcctc
    12361 ctaattttta ataaatctat attaacaggc tttcttgaaa aattaatgat taatctaaat
    12421 aaacagataa tatttataaa accatccata tttttcattg taagactttg gatatgttat
    12481 atttatagta aaattaaagg agtgagcgaa aatggatgcg atgagtttta ttttactaaa
    12541 ctttgaagaa gtgagacgtc gaagtataaa agtatggatg tcaatacctc aagataggtt
    12601 agactggcgc ccagatccag aagcaatgtc atgtagggag atgatacatc atgttcttga
    12661 aggagaatac ctctatcatc aaatgttaga aaaacccctt aacctttctc taacagagaa
    12721 gcataatcct tataaagcag taacatttag ttctgttgaa gaagcattaa agtttgctca
    12781 accatatagg gaaaaattta tagagttttt aggactcctt aaagaaaagc agctaacaga
    12841 gataaaaata gaccgatcgg agatgggata tattcgagag ttaggggaca tgttgttacg
    12901 agttgcttat catgaatcag tacatacagg acaactttta gattatttaa gaactgcaaa
    12961 agttaaacgt cctataatat gggattaagt ttctgattaa tctcccgttg ttttattttt
    13021 ctctgctcta aagaacgctc atacaaagcc ataaagttgt ttacttaaag ggggaagaac
    13081 cattgtgaat aatgaaaagc agttacttaa atttcagacg tttcaacaac ttcacaaaag
    13141 accctatact tttgtattgc ctaatgcttg ggatgttatc agtgctaaaa tttttgagaa
    13201 aaatgggttt ccagctattg caacaaccag tgctggcata gcaatgtctt taggatataa
    13261 tgatggtgaa aaaatccctt ttgaacaaat gttaggagct gttaagagaa ttattaattc
    13321 cgtcaatatt cctgtcagtg tagacctaga gtctggctat ggaaggtcta ctaatgaagt
    13381 ggtagataat acaagacaaa ttattttagc aggaggaatt ggtataaacc tagaagatgg
    13441 gacgggagat ttagatagtc ctattttaga tgtttcgttg caagtagaaa aaatatctga
    13501 aattagatta ctatccaaat cgctaaaagc cccattattt attaatgcgc gtactgattt
    13561 gtattggtta aatacaggag atccggatct aagacttcaa gaagccatca ataggggaaa
    13621 agcatatcaa aaggctggag cagattgtat ttttattcct ggactatatg acatggagtc
    13681 cattaaaaaa gttcgacaag aactatcttg tcccattaat cttttgtctg ggccaaactt
    13741 gccttctatt aatacattat cccaagttgg tattgaaaga ataagtactg gatccgcccc
    13801 ttttagagca actgctacat tattacaaag aattactgaa gatatccttt atgataataa
    13861 ttttcaacga ttaatgaatg ataatatgag ttatgatgcg ataacagaac taattgggac
    13921 tcctgaatag aattgatcaa atttaattta gcttgccttt atagaactgg taggactact
    13981 gaaatataca gtagttcttt tgctgtttga actatgttta aacagctacc ataaaacgtt
    14041 ttatagatcg ttgatataac aactattttc tttcaggtga ttgaagatat gatgtataaa
    14101 aatctcccct tttatacatg tgctcgtcat ggaaaagata gtcgttatcg tataaatatg
    14161 ttatcattta tctaaagtta tagagttatc tcaaaaagca acctcactcg aatgccatta
    14221 aagactgagg ttgctttttc ttttttaaat aaaattaaaa taattgtgtt aactcaaaag
    14281 gtaagttagt tgaataaaat attaatttac tatctaagta gtaaccgcgt ttttattcta
    14341 caaggaggcc tagaatgatg aatctaatcg ataagaaagt tacacacaag cattttggaa
    14401 caggtactat agttaaatat aatgattcta gtattgaaat aaatttcgca tcagaaaaca
    14461 aaaaatttgt tttccccgat gtatttggaa agcacctaaa actgcatgat aaaagtgttg
    14521 ctcattccct ggaaaaaatt ttagaagaaa aggaaacgga acgaaaagag gaagaatgga
    14581 agaaggaaga agaaaaaaaa ttacagcgaa aaaatcagga acttcgctgg ggacatgaaa
    14641 aacttatgaa aaaccataaa cttcatcccg aatcacaaat ggttttttgg tgcgacacag
    14701 aagaacagaa tagttctttt tcagagtgga cggttttttc aggcgtaata aaaagtggta
    14761 attacaaggg gaagccaacc aaacccaccc gcctacatca aaatagcgct gtcttgttaa
    14821 cagcaataga ttccagtatg cctgaaaaag acagacgcat cttaggtgtc tatatggtca
    14881 atgaaaattt tatcggtaag ctttgcgaag atggatatat tcctgctcat tcaacgtaca
    14941 gactccaact tacagaacaa gaatccaatc agatgctttt ctggaaatat tatataaacg
    15001 aaaagaactc ccaaaaaatg gtgtggaata caggtaaata ccgttatttt gataattcat
    15061 ggatggctca aattttgctt gatatagttt cattgaaaag tgactcaaac gaacgagagc
    15121 aggcacagca attttttgaa catttttgca aaatgaattt gataacggat caagatttac
    15181 caaagcctaa tggcgcatta atgcgtattt agacgttagc ccacaggtaa ttaattaaga
    15241 atgaaaaaag acagacgcac ttttctgacc cattgctaat gacgaacagt gccaggctct
    15301 gtttgccaat aaaagcgtaa ataaagaatc tactgctacg tgtaaagtaa aacaagcagg
    15361 ctcttaataa ttaagaatct gcttgttctg ctctacataa ggactatcag taagttattt
    15421 tatttttttt ctaaacaggt acatatctaa caacatatca acaagcaata aattaagcta
    15481 gtacgtttat tttaatatac agttgagcta gatagaatat ctttatgaaa acgtactgtt
    15541 tcagaactat aggcagtcat atttagtaca ctttttacag aatatgatat gaggactacc
    15601 ccatctttca gtgtactcac ccaacaagga tgaaagggta ttaaaaaata agattgctta
    15661 tcaagttttt ctaaaacaaa tggtgctggg ggagcttttt atttttgcta caattcttag
    15721 aaagaagtat ttgatattat taaaaacgct agttacagca attataggag gtattcattt
    15781 tttaaacttt tgtaaaatta tccacttgag agaaagcaga caaagaaaat ataaatcata
    15841 ttatacatag cttttccttt tctattatct tccttaccct actcctagtt actgaaactt
    15901 tcccttaaaa aacaaagaac agctaatatt tttaaataag gagtttttaa atgagtaatt
    15961 atgaaaaaac taagaataac aaaaatataa ttttaggtat tatatatgct ctaattgtaa
    16021 tatctgtata tgcatttaac ttacatctac agatagcttt gtctatactt actcttgtca
    16081 taatatttga acttgtaacc tcaataaaag aagatgacaa agctaaaaat gttataaaca
    16141 ttattaccat atgtttattt atacttggaa ttgtttttct ctaactagta cttactaata
    16201 gctaacaata taaaaagagc aaaggcttct tataaatgaa gaagcctttg ctctttttat
    16261 atctttccca ctctttttct agacggttag tatgcttttg aatatcgcta acatgtttta
    16321 atttaagatt aaggttgcct gatatagata gtcagcaaca gtatactttt acgggctttt
    16381 ttttgagata agaaaactat cattttccag tttaagtaag ttgaagctcg tccttagtga
    16441 accataaaaa atccccacta aatatagtgt aaccctcact ctcaaagagt aatttcttta
    16501 cactatactt agaggagata ataccaaatt ttactgtttt tcttgcatta cttgagctac
    16561 ttcagtaaaa attacaccta agccatgtgc acctgcgtcc ggataaccaa ggcttctttc
    16621 tccaaccgta ccagcacgcc ccatacgtgc aacaatctgt tcagttgatt ttgctccttc
    16681 aacagcagct tctgctgctt tgacaagcgc atgtttcaag ctttcacctt cagcagcact
    16741 tgacgttaac gtatctgcat aaggtactaa ggcatcaatt aaagttttat ctcccacaac
    16801 agctccacgt ccaaaagaac gttctcctgt tgcttgaatt ccttttacag acgcttggat
    16861 catttcagca aattctgtta aagtcagttc ggttttagat tcagtgttct ttccagccgc
    16921 acgaaatgct gatccccaaa taggccctga tgccccgcca caatgctcca tgataacgat
    16981 ggaacaagcc tctagaaagt tgccaatatc atttgattta ttttctagaa tttcgtgcca
    17041 ttcagctttc aactgtttga accctttagc aacactcata ccaaagtctc cgtcaccagc
    17101 atgggaatct aattcacaaa acggtatttc atttctaata atacattcac tcatttggtc
    17161 caccataaaa ataatattat tcaacgttag tcgattttcc gttactacgg aatattcttt
    17221 gttggtttgc acctcataag acactttcgg ggcctccgcg gtaccgatga tttcatcata
    17281 agtgacagct ggtacttcac ctttaagttt tagcgcagga gtatcgcact cttcagatag
    17341 aagactcttt aagttttcat ctaatttcat aattgaaata gaagcaccgg ccatatcaat
    17401 ggctgtcata tagtttccga caaacgttct tgcaatcgtt acttttctac gtgacagttc
    17461 acgaatgaca gaatgattta ataaatacag ttcttgaaga ggtgtgcttc caaatccatt
    17521 aattaggaca gccacttctc cttttccatc ttctatccct atttctttta ataatgccgt
    17581 taccatgcgc tctgcaagtt cgtctgctga aataattttt tcacgacgga taccaggttc
    17641 tccatgaatg ccaacgccaa attccatctc atcttctgca atctcaaaag taggagttcc
    17701 tttagcagga actgtacaag aagttagtcc aataccaatg cttttaacat ttgcaatagc
    17761 gctttctgct gcctctttca cttttggtag atcatatccg cgttctgctg cagcacctgc
    17821 tattttatgg acaagaacag taccagccac tccacgtctt ccaactgtat ataagctgtc
    17881 ctgtacagca atatcatcat ctacttttac ataatccact tgaattccat cttcttctgc
    17941 tagataagca gcattcttga aattcatcat gtcaccactg tagttcttaa tcacgagtag
    18001 agttccctta tcgctagctg tttctttaat ggcttgataa atctgtattt gagaaggtga
    18061 ggcaaataca tctccacaaa ctgctgcatc taacataccc ttcccaacat acccagcatg
    18121 agcaggttca tgtccactgc cgcccccgct aattatgctt actttatttt tattaatttg
    18181 ctctttttta atgactttat atttttcaat taaattcagt tccggatgtg ctagaaccat
    18241 tcctttacac atctcaatta cgacattttc tggtttatta ataatttttt tcatcttata
    18301 ttaacctctt cttataatca tttccaattt gatctgctgc aaggatagct gcagctactg
    18361 actctggagt aacagggaat ggcatagaat aaatagattc ctctggaata caagctttgt
    18421 tagctacttc cattaactct tcttgtgtaa tttgttctac tccaatatca gcaagacaaa
    18481 ctgggagtcc tactgctaaa cagaaatcta acacttcttc tatttcctca gttggcgcat
    18541 tttccaatac caattgtgct aatgtaccaa aagctacttt ttcaccgtgg aagtagtggt
    18601 gcgcactttc taaaagagta agcccatcat gaatggcatg tgctccagct aatccaccac
    18661 tttcgaatcc taatcctgat aataagatat tggcttcaat aatgttttct aaggcgggag
    18721 taacaacatt atgatcgctt gctgcttttg ctttaacccc gtcctctaat agagtgttgt
    18781 agcaatgttc tgctaaaact agcgctgcat ttgtaccttt tgctggtgca catacgtcct
    18841 cacgcactcc acaaggtaat ccagcattta cattagagaa agaacgtgct gttgctcttg
    18901 cttcgaaata agtggataac gcatccccca taccagatac taagaatcta gtaggtgcat
    18961 tagcaatgac tgttgtatca attaatacaa cacttgggct ttgcttaaag tatgcgtaat
    19021 catcaaaagc accctctggt gtgtagataa ctgcggaatg acttgtaggt gcatccgtag
    19081 cagcaattgt tgggacgata attaatccct ctccctctgc tacacatttt gctgtatcaa
    19141 ttgccttacc accacctaaa ccaattgtac aatcacagtt gttttctttt gctatttcct
    19201 gcaatctaga aatttctggt cttgagcact cgccattgaa tccgccttca aaaaatgtaa
    19261 taccatattt ttctgctgtt gcatctaatt tgccttgtac acgtttaata tcttcaggat
    19321 gtgctattaa aagtgcagag gtaccaaatg ttttcacaaa ataacctaaa tttaaaattt
    19381 catcttcacc ttgaatgtat tttgaaggac taataaatgc ttttctcata aataaaacct
    19441 cctaattaac tttgtaatcc cttacaatcg gaattttact atcttttgtg taagattaca
    19501 ttagttttaa atgacgagtt tttacaactt tttatcgtat ttttttgctc tcttcattta
    19561 atatggtatt attatgacat tatttcaggt aaaaatacta ctaagttcac gttgaaaaga
    19621 ggttaccttt atcgctgaac actcttttat tttacgaact caggaataaa ataaaggtat
    19681 tctcaaatag gtaatccaaa ctaccatatg ggaagtagaa gcaagggaga taaaacatca
    19741 ttgaaaacta cttacatgaa tttagataaa atactagatt taaagaaatg gcgaaacctt
    19801 caagattcgc ttgcgcttgt tacaaaatta gcgattgtca cggttaacta taaaggagtg
    19861 cccatttctc aacacagtgg tgtacgtcca ttttgtgaga ctatgcgtaa aaatcctaat
    19921 acggcaaaac tttgtgaaaa gtgcgatgcg cgagggggac tagaggcagt tagaaccaat
    19981 gctccgtata tctatttatg ccactggaac attgtggata ttgccattcc cattataatt
    20041 gatgataaat atgtaggcgc catcatggcg ggagaagtga aaattgagga tggtgacaaa
    20101 catcctccct tggaacaaat gcttactgtt ccatcgaagg gatttgaaca gaagttaaat
    20161 aataatgagg aaatacagcg tctatatgat tctattccta ctctatccta tacagaaatt
    20221 aacgacgctg ctcaaatgtt atataggctt tgtaactaca ttgtagagga agctatgaat
    20281 aaaaacttga ttttagaagt atatgaaaag atggctccag taaaagagga cctttctact
    20341 gaagatattt cttacggtta ttctctagat aatattcaaa atataaaaaa ggaactagcc
    20401 aacacagtta ctaatgctta cgtaaaaacg tctattaacg atcacttagt atgtaaaaac
    20461 aaagtattac agccagcctt tgactacatt tataaacata aaagagaaaa aatttctcaa
    20521 caaaagatgg ctgatttatg tcacattagc acaggttatt tcagccgtgt atttaccaaa
    20581 gaaacgggta tgaatttttc tgtttttatt tcgctcaaaa aaatagagtg gtccaaacaa
    20641 ttattagaaa aaacggattt gtctattgca cagattagtg atgaattagg ttttaatgac
    20701 cctggatatt acatcaagac atttaaaaaa tatgaaaata taactcctaa cgtctatcgg
    20761 aaatattatc aagaaaagat gtaaagtttt cattcctgac gtaagaaaca tataactttt
    20821 ccatactgat actagtattc tgaaaaaggg tgcttttcag tatggaaaag caatgagaca
    20881 cagtcatgtg ctggccttta cgctataaat aatatataaa tctctattcc gcatgcttat
    20941 aaacattggg tctcaggcct tcaaatattg ggatgttttt ttgttgttct gtaactttct
    21001 gtatatgaat ttctgctgtt atgatttctt ctttgtctct cccctctttt ataatgtttc
    21061 catttggatc gatgaccatt gaatgtccag cgtatgtgtt gccggca
//
