LOCUS       NZ_AP019791            25605 bp    DNA     circular CON 25-APR-2024
DEFINITION  Rubrobacter xylanophilus strain AA3-22 chromosome, complete genome.
ACCESSION   NZ_AP019791
VERSION     NZ_AP019791.1
KEYWORDS    RefSeq.
SOURCE      Rubrobacter xylanophilus
  ORGANISM  Rubrobacter xylanophilus
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1
  AUTHORS   Tomariguchi,N. and Miyazaki,K.
  TITLE     Complete Genome Sequence of Rubrobacter xylanophilus Strain AA3-22,
            Isolated from Arima Onsen in Japan
  JOURNAL   Microbiol Resour Announc 8 (34), e00818-19 (2019)
   PUBMED   31439702
  REMARK    DOI:10.1128/MRA.00818-19 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022875)
  AUTHORS   Miyazaki,K. and Tomariguchi,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2019) Contact:Kentaro Miyazaki National Institute
            of Advanced Industrial Science and Technology; 1-1-1 Higashi,
            Tsukuba 305-8566, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Coverage                   :: 758x
            Sequencing Technology             :: NovaSeq6000; GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007164525.1-RS_2024_04_25
            Annotation Date                   :: 04/25/2024 02:58:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,032
            Genes (coding)                    :: 3,015
            CDSs (with protein)               :: 3,015
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 9 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:10:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2384351
            Orig. end                         :: 2409956
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP019791.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated at DFAST https://dfast.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..25605
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="9"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..25605
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..25605
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:15605](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..15605
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    complement(263..276)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.32"
                     /tool="antismash"
     gene            complement(453..677)
                     /locus_tag="RxyAA322_RS12170"
                     /old_locus_tag="RxyAA322_24280"
     CDS             complement(453..677)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RxyAA322_RS12170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="RxyAA322_24280"
                     /product="hypothetical protein"
                     /protein_id="WP_143528566.1"
                     /transl_table=11
                     /translation="MSAALALGLLLLLAGFGGVGYGLYALLHGGRGQSGGIGPLPERGV
                     HVIAGLRMLVVGAVCLVAGGYLLWSYFGG"
     gene            complement(684..2018)
                     /locus_tag="RxyAA322_RS12175"
                     /old_locus_tag="RxyAA322_24290"
     CDS             complement(684..2018)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1272: TPR
                     repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /locus_tag="RxyAA322_RS12175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24290"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_143528567.1"
                     /transl_table=11
                     /translation="MDHRELIEQGEALAGEGLIEEALERFEQALQRAPENPEVVEAVGR
                     ALLGLGRLEEAEASFLDALEMDPGWVAPRMGLAMVAMRRDEPFKIVHHLERAIEADPEQ
                     PDAYVELGRYYGLMGEPLLARATFERWLARHPDDADMLINAGLTLFDAGDYAPALEFFE
                     RAVEAAEDAEQRLGALTFRANALDMLGRYEEAVASYEAVIAEDPQWWEAHANLGICHAR
                     SGHLREAEAAFRRGLEACPGSPEIRDELAAHLLAHGGDVREALSLAEEAVALGSDEIRH
                     LYTLGEARLANGDEEGAIEAYRTVLRLDPENPEAHLELGILHDRRGERERAERHFVESL
                     KADPGNPRTLYSYANVYYTAGELETAEELLVRALAADPGYSPALSALASIRARRGEYAA
                     SLDYLEKAIEAGERDAEHFKRALEFAPLRQDPRFRRLLSRMSEGD"
     misc_feature    2042..2053
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 16.97"
                     /tool="antismash"
     misc_feature    complement(2049..2062)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.36"
                     /tool="antismash"
     gene            2116..2568
                     /locus_tag="RxyAA322_RS12180"
                     /old_locus_tag="RxyAA322_24300"
     CDS             2116..2568
                     /EC_number="3.1.2.-"
                     /GO_function="GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 4HBT_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /locus_tag="RxyAA322_RS12180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24300"
                     /product="acyl-CoA thioesterase"
                     /protein_id="WP_172620836.1"
                     /sec_met_domain="4HBT_2 (E-value: 5.2e-21, bitscore: 72.8,
                     seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLEIRYQDLDPYGHVNNAVYLQFFESLRFAYWRALAGVVGATE
                     FEAGGIIPGGRYVIAETTVRYRSELLFGDELHGAASIRSVGNSSFVMDYELRKGVSFEG
                     GAPVADGSAVHVFYDMRSRRSRPRPDWFLPAVAELEGRPEEGFLLD"
     gene            complement(2911..3585)
                     /locus_tag="RxyAA322_RS12185"
                     /old_locus_tag="RxyAA322_24310"
     CDS             complement(2911..3585)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1130: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /locus_tag="RxyAA322_RS12185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24310"
                     /product="IS256 family transposase"
                     /protein_id="WP_172620837.1"
                     /transl_table=11
                     /translation="MVVSAVRDDGYREILGLEVADVESEATYQELFRALKERGLKGVEL
                     VTSDEHKGLKSAIERHFRGASHQRCQFHYAKNLLGMVGLDKRAELAEGLRGIFAAPTKE
                     LALRAAEELADRWRGSHEKVAEHLEEHVEECLACLAFPESHRRRIRTTNGLERFNQEIK
                     RRTRVVRIFPNRESCLRLVTALAVEQSEEWVTGRRYLDMEQLKKRRSKERAAKEEVILT
                     QR"
     misc_feature    complement(3031..3033)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3535..3537)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3551..3820)
                     /locus_tag="RxyAA322_RS16050"
                     /old_locus_tag="RxyAA322_24320"
     CDS             complement(3551..3820)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /locus_tag="RxyAA322_RS16050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24320"
                     /product="transposase"
                     /protein_id="WP_274596109.1"
                     /transl_table=11
                     /translation="MEMYIEGVSTRKVKEVTEKLCGTAFSKSTVSRLATELDSELKEWR
                     SRPLTAEAYPYLFVDALNARRCELAAGSSRRGRLGRFGGSRRRL"
     gene            complement(3820..4248)
                     /locus_tag="RxyAA322_RS16055"
     CDS             complement(3820..4248)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /locus_tag="RxyAA322_RS16055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_342212015.1"
                     /transl_table=11
                     /translation="MLHLRLAQQLRKSYPQLIGHRVSLLFRLTLFQEPHGGRLRQRPFL
                     HTSWDTTHFLGHYRFLRGIVEGMLQQMLEAEITEHLGAAPHQRTENRKGYRNGYKPRKL
                     KSRVGTLDLLVPQDREGTFSTQLFARYQSATRRRSLWR"
     gene            4189..5427
                     /locus_tag="RxyAA322_RS12200"
                     /pseudo=""
     CDS             4189..5427
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558308.1"
                     /locus_tag="RxyAA322_RS12200"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS256 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MADELRVGLSELLRKAQMQHDTDFLRESVRTLRERR"
     misc_feature    5525..5545
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.86"
                     /tool="antismash"
     misc_feature    5560..5574
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     misc_feature    complement(5637..5651)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.57"
                     /tool="antismash"
     misc_feature    5950..5963
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.53"
                     /tool="antismash"
     misc_feature    complement(5952..5965)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.49"
                     /tool="antismash"
     misc_feature    complement(5955..5968)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.94"
                     /tool="antismash"
     misc_feature    5957..5970
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            6028..6429
                     /locus_tag="RxyAA322_RS12205"
     CDS             6028..6429
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RxyAA322_RS12205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_143528572.1"
                     /transl_table=11
                     /translation="MSQRTETAERWVEDFRRVTNEDPEIQAHGKYFSCSFLLDMEQSRF
                     LVKMHEGKVEEILTNPGPLDERYQFALRASAETWRKFGEEVPEPMYHGIWSASFQRDLR
                     LEGDLLVLMQNLRCFTRQFELLRQIGVPV"
     gene            6463..7317
                     /locus_tag="RxyAA322_RS12210"
                     /old_locus_tag="RxyAA322_24370"
     CDS             6463..7317
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (32-145). E-value:
                     8.4e-20. Score: 63.3. Matches aSDomain:
                     nrpspksdomains_RxyAA322_RS12210_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /locus_tag="RxyAA322_RS12210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24370"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_143528573.1"
                     /sec_met_domain="PF00561 (E-value: 2.5e-15, bitscore: 54.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 9.5e-11, bitscore:
                     40.2, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQFSPVTGRYVTIDVLGDECKVFFLENGEGQPLVCQHTAGCHNH
                     QWRDLLEDEEITRNYRVIAYDLARHGKSDPPKNREWWKEEYRLTADYYTAFIVAFCDAL
                     ELEDPIFMGSSFGGNVALQLALRHPDRFKAVIPVEAAEYSPGFFLDWWQHPQANAAQVC
                     ASGVWDLMAPQSPEKDRWATWFYYSQGSEAFRGDLYFYSVDHDLRGKLDQIDGEKCPVI
                     MLTGDYDYLTTPEDSQRTADQIKGGEFIEMKGIGHFPMSENYPLFKRYLQQALQRIENR
                     TGG"
     aSDomain        6559..6897
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_RxyAA322_RS12210_Abhydrolase_1.1
                     "
                     /evalue="8.40E-20"
                     /label="RxyAA322_RS12210_Abhydrolase_1.1"
                     /locus_tag="RxyAA322_RS12210"
                     /protein_end="145"
                     /protein_start="32"
                     /score="63.3"
                     /tool="antismash"
                     /translation="PLVCQHTAGCHNHQWRDLLEDEEITRNYRVIAYDLARHGKSDPPK
                     NREWWKEEYRLTADYYTAFIVAFCDALELEDPIFMGSSFGGNVALQLALRHPDRFKAVI
                     PVEAAEYSP"
     CDS_motif       6793..6837
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_RxyAA322_RS12210_0001"
                     /evalue="4.20E-03"
                     /label="NRPS-te1"
                     /locus_tag="RxyAA322_RS12210"
                     /protein_end="125"
                     /protein_start="110"
                     /score="9.7"
                     /tool="antismash"
                     /translation="FMGSSFGGNVALQLA"
     gene            7438..8013
                     /locus_tag="RxyAA322_RS12215"
                     /old_locus_tag="RxyAA322_24380"
     CDS             7438..8013
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /locus_tag="RxyAA322_RS12215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24380"
                     /product="hypothetical protein"
                     /protein_id="WP_244299739.1"
                     /transl_table=11
                     /translation="MNEANFSPWLVGIMLSGALAAAMSTGSNLAHTASTVLVRDLFVAV
                     FRQDMPERQVVLLTKIFVVVISVVAYVLALFNPASLVGLLLGAYGAVVQFFPLIVAVFF
                     WKRATKAGAFAGLISGSAVMLYFSFLAPPPFEIHAGIWGLLANTVALVAVSLLTEPMPE
                     EHVERFVEGSKASLEEISGPPRPDASTA"
     gene            8065..8874
                     /locus_tag="RxyAA322_RS12220"
                     /old_locus_tag="RxyAA322_24390"
     CDS             8065..8874
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566069.1"
                     /locus_tag="RxyAA322_RS12220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24390"
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_143528574.1"
                     /transl_table=11
                     /translation="MELRDLEAFGEPGAMVYLADCVELMRLMPPGSVDAIFADPPYRLS
                     GGGVTVRAGRLVPVDKGGWDRPLGPRGNHEFNLRWLREARRILKPGGSLWVSGTHHIIF
                     SLGFALQTLGFRIINDIVWQKPDPPPNALRTAFTHAHETLIWASKGRGHTFNHELLDGP
                     APASRISSVWRMPPPPSRERLHGYHPTQKPLRLVRRALLASTREGELVFDPFTGSGTTA
                     VAAKELGRFFVGAELEREYAELAGRRIRAAGRGTAVQPARGSGNPAG"
     gene            8944..9105
                     /gene="mftA"
                     /locus_tag="RxyAA322_RS12225"
                     /old_locus_tag="RxyAA322_24400"
     CDS             8944..9105
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: protein motif:HMM:TIGR03969.1"
                     /locus_tag="RxyAA322_RS12225"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24400"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_143528575.1"
                     /transl_table=11
                     /translation="MDVTRESTSAAATPVREDDRPEEAARTGGGESGAVEEEILIEEVS
                     IDGMCGVY"
     gene            9118..9975
                     /locus_tag="RxyAA322_RS12230"
                     /old_locus_tag="RxyAA322_24410"
     CDS             9118..9975
                     /NRPS_PKS="Domain: PKS_KR (12-185). E-value: 3.6e-14.
                     Score: 45.0. Matches aSDomain:
                     nrpspksdomains_RxyAA322_RS12230_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566068.1"
                     /locus_tag="RxyAA322_RS12230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24410"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_143528576.1"
                     /sec_met_domain="adh_short (E-value: 1.2e-31, bitscore:
                     107.6, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEGRRTLLLEDRVALITGAARGQGRSHALTLAREGADIIACDICE
                     PISSAPYPMGSREELEQTARMVAELGRRVVAQKADVRSLEQLRAVVERGLSELGRIDVV
                     CANAGVISYAPSWRITEEQWRETLETNLTGAWHTCRAVIPHMMERGEGGSIILTGSTAA
                     VVGFENLAHYVSAKHGLVGLMKTLANELAPHRIRVNIVHPTSVDTAMIHNEATYRLFVP
                     GADRTPTREEAARAFRTLNALPVPWVEPEDVSNAVLWLASDHSRYVTGQQLRVDAGAAE
                     KARR"
     aSDomain        9154..9672
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_RxyAA322_RS12230_PKS_KR.1"
                     /evalue="3.60E-14"
                     /label="RxyAA322_RS12230_PKS_KR.1"
                     /locus_tag="RxyAA322_RS12230"
                     /protein_end="185"
                     /protein_start="12"
                     /score="45.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALITGAARGQGRSHALTLAREGADIIACDICEPISSAPYPMGSR
                     EELEQTARMVAELGRRVVAQKADVRSLEQLRAVVERGLSELGRIDVVCANAGVISYAPS
                     WRITEEQWRETLETNLTGAWHTCRAVIPHMMERGEGGSIILTGSTAAVVGFENLAHYVS
                     AKHGLVGLMK"
     CDS_motif       9160..9228
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_RxyAA322_RS12230_0001"
                     /evalue="1.40E-02"
                     /label="PKSI-KR_m1"
                     /locus_tag="RxyAA322_RS12230"
                     /protein_end="37"
                     /protein_start="14"
                     /score="8.2"
                     /tool="antismash"
                     /translation="LITGAARGQGRSHALTLAREGAD"
     gene            10001..10279
                     /gene="mftB"
                     /locus_tag="RxyAA322_RS12235"
                     /old_locus_tag="RxyAA322_24420"
     CDS             10001..10279
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03967.2"
                     /locus_tag="RxyAA322_RS12235"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="RxyAA322_24420"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_143528577.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 1.6e-15,
                     bitscore: 54.6, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLRDEARCSLAPQVSVRAERFGGLIYRYDDRALLFLRSRPLVEL
                     LLGLDGTKTLKEALEELAAERDLGVRELRAVKDALQKLEERGIVREL"
     aSDomain        10019..10267
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_RxyAA322_RS12235_Mycofactocin_RRE.1"
                     /evalue="1.50E-17"
                     /identifier="RREFam016.1"
                     /label="RxyAA322_RS12235"
                     /locus_tag="RxyAA322_RS12235"
                     /protein_end="89"
                     /protein_start="6"
                     /score="54.6"
                     /tool="antismash"
                     /translation="ARCSLAPQVSVRAERFGGLIYRYDDRALLFLRSRPLVELLLGLDG
                     TKTLKEALEELAAERDLGVRELRAVKDALQKLEERGIV"
     gene            10269..11354
                     /gene="mftC"
                     /locus_tag="RxyAA322_RS12240"
                     /old_locus_tag="RxyAA322_24430"
     CDS             10269..11354
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03962.1"
                     /locus_tag="RxyAA322_RS12240"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="RxyAA322_24430"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_143528578.1"
                     /sec_met_domain="PTHR43273 (E-value: 3.5e-40, bitscore:
                     135.6, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.7e-22, bitscore: 78.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 8.8e-08, bitscore: 30.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 1.8e-08, bitscore: 31.6,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.5e-15, bitscore:
                     54.8, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 6.6e-06, bitscore: 24.0,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYELLREGLDAPICLTWELTYHCNLRCAHCLSDSGMGREDELTT
                     GEARSLIDEWAEMKVFYVNVGGGEPMTRPDFFELMDYCLQRGIGVKFSTNGTLLDDAAA
                     DWISSRPYLDVQISLDGANPETNDPVRGTGSFKRARRAMERLAERSFPFKINTVLTRHN
                     FPQLDELYALAKGYGARLRLTRLRPSGRGVRVWGELRPTREQNRELYRWLHAHPDVLTG
                     DSFFHLSAYGEPLAGMNMCGAGRIVCCVDPVGEVYACPFVLAPEFSAGNVRDPGGFARV
                     WRESPLFRRLREWQVGGECRSCNAYHLCHGGCMAVKHFTGRPLDSPDPDCVFGRGEEPS
                     RGQFVALGSGKRGAGAASALE"
     gene            11395..12522
                     /locus_tag="RxyAA322_RS12245"
                     /old_locus_tag="RxyAA322_24440"
     CDS             11395..12522
                     /NRPS_PKS="Domain: PKS_ER (14-368). E-value: 4e-16. Score:
                     51.0. Matches aSDomain:
                     nrpspksdomains_RxyAA322_RS12245_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566065.1"
                     /locus_tag="RxyAA322_RS12245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24440"
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="WP_143528579.1"
                     /sec_met_domain="ADH_N (E-value: 3.1e-27, bitscore: 92.1,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 4e-25, bitscore:
                     85.8, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSRAALLYGEGQEFRVEEIEVDDPKQNEVLVHLAATGLCHSDYH
                     MVTGEYGPLHFPMIGGHEGAGVVEKVGPGVTRVKPGDHVLLTFIPACGHCRFCSMGLSF
                     LCDRGAAILQGPQLDGTFRMHTADGQDAGQFCCISTFSEYTVVPEASIVPIADDLDLTK
                     VCIVGCCVPTGFGSAVNRADVQPGETVVIFGIGGVGINAVQGAAVKGASKVVAVDPVDF
                     KLETAEELGATHTINPNERDPVQTVVDLTNGVGADKAIITIDNVLPEHVGQAFRAVRKG
                     GRVVVTGVARYDHQHIDVSPYELTLFAKEVVGTMYGHSPVNSDLQRYLELYRTGRLKVD
                     ELITRTYSHDEINQGYRDMLDGKNIRGVIVYDWAR"
     aSDomain        11437..12498
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_RxyAA322_RS12245_PKS_ER.1"
                     /evalue="4.00E-16"
                     /label="RxyAA322_RS12245_PKS_ER.1"
                     /locus_tag="RxyAA322_RS12245"
                     /protein_end="368"
                     /protein_start="14"
                     /score="51.0"
                     /tool="antismash"
                     /translation="FRVEEIEVDDPKQNEVLVHLAATGLCHSDYHMVTGEYGPLHFPMI
                     GGHEGAGVVEKVGPGVTRVKPGDHVLLTFIPACGHCRFCSMGLSFLCDRGAAILQGPQL
                     DGTFRMHTADGQDAGQFCCISTFSEYTVVPEASIVPIADDLDLTKVCIVGCCVPTGFGS
                     AVNRADVQPGETVVIFGIGGVGINAVQGAAVKGASKVVAVDPVDFKLETAEELGATHTI
                     NPNERDPVQTVVDLTNGVGADKAIITIDNVLPEHVGQAFRAVRKGGRVVVTGVARYDHQ
                     HIDVSPYELTLFAKEVVGTMYGHSPVNSDLQRYLELYRTGRLKVDELITRTYSHDEINQ
                     GYRDMLDGKNIRGV"
     gene            12533..14395
                     /locus_tag="RxyAA322_RS12250"
                     /old_locus_tag="RxyAA322_24450"
     CDS             12533..14395
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566064.1"
                     /locus_tag="RxyAA322_RS12250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24450"
                     /product="AAA family ATPase"
                     /protein_id="WP_143528580.1"
                     /transl_table=11
                     /translation="MDLIRIHDEVRRSIVGREEELQLLLAALAAGRDVLLEGPPGTSKS
                     TLLRAVTRASGSSLRFVEGNADLTPAKLVGHHSPSRVLQEDYGPENFVYGPLPQAMREG
                     GILYIEELNRVPEDTLNALLTAMAERELTIPRVGVVRAAEGFRVVAAMNPFDNIGTARL
                     GGAVTDRLCRVRLDYQPEDEERRIVARRTGSEDAFLVGLAVRVARLTRSHPDVRMGASV
                     RAAIDFVLVAEQLLRLRGLAAPDAPRDEAVRRTLVSAAQTALSIKIAVREASGRSADEV
                     VEEVVRAALAGPRDPEEPPEPSSNGAPEGVPRAPRPRSGGETRRTVGQGSLGSLARSRP
                     NLAGRLAAERSLDAAALEAAVRDEGGEPLEVLGELVDLYDRADLRELARRLARELVLRA
                     ARRDAAGRGGRGRLASSPYRGQAAELDLDRSLERALLPPAPRDEELFVLDRRHHRRAYA
                     LVLDASGSMKGGALLRAALAASAFAVRADPDPFAVVAFWREVAVIKHLEERLPLEVLLD
                     RVLSLPARGLTDLCLGLRTGLEELEGSGLRERTGLLFSDGMQTTGSPAEPVAAAFPQLH
                     VVAAAQSPESLARCRHLADLGRGRYAPVRSISEIPAAINSCLAA"
     gene            14406..15605
                     /gene="mftD"
                     /locus_tag="RxyAA322_RS12255"
                     /old_locus_tag="RxyAA322_24460"
     CDS             14406..15605
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566063.1"
                     /locus_tag="RxyAA322_RS12255"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="RxyAA322_24460"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_143528581.1"
                     /sec_met_domain="TIGR03966 (E-value: 2.8e-211, bitscore:
                     698.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASSGEWFESVAEAQRRARRRLPRSVYRALLAGTEKGLTLRDNLA
                     AFDELGLVPRLVGFPGGGFELSTRVMGLEVSMPVLISPTGVQAVHPEGELAVARAAAAR
                     GTAMGLSSFASKPVEEVVSANPRTFFQTYWIGGRERMLGIVERAREAGAAGMILTLDWS
                     FSHSRDWGSPYIPDRIDLKTMLRFAPEVALKPGWLLRYANARSLPELTVPNMTPSPKDP
                     APTFFGAYAEWMQSPPPTWEDVAWLRERWGGPFMLKGVMRVDEARRAVEEAGVTAISVS
                     NHGGNNLDGVPASIRALPAIAEAVGDRVEVLLDGGIRRGGDVVKALALGARAVMIGRAY
                     LWGLAAGGQAGVENVLEILRSGMESTLRGLGKSSIHDLDRDDLIVPAGFERRLGEGDGE
                     "
     gene            15595..16353
                     /gene="mftE"
                     /locus_tag="RxyAA322_RS12260"
                     /old_locus_tag="RxyAA322_24470"
     CDS             15595..16353
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: protein motif:HMM:TIGR03964.1"
                     /locus_tag="RxyAA322_RS12260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24470"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_143528582.1"
                     /transl_table=11
                     /translation="MGNRLAEMSWPEVERAVAAGATTVLLPLGATEQHGPHLPLGTDTV
                     RAEALAGLLAERLPGALVAPALPFGCSDEHAGFPGLLGVGKETLARLIVDLARRMAGWG
                     VERLVLLSAHGGNGEALSLARAMLRSELPGLAVWAPEDLTSLSPATAGVARRAGVPEGA
                     LGLHAGEGETSELLHLRPELVRRERARPGYTGDMESVVKILKAGGLRAAHRSGVLGDPT
                     LASAGRGASYLEARAEEIARAVLGDPDGGR"
     gene            16340..18688
                     /gene="mftF"
                     /locus_tag="RxyAA322_RS12265"
                     /old_locus_tag="RxyAA322_24480"
     CDS             16340..18688
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /locus_tag="RxyAA322_RS12265"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24480"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_143528583.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 8.1e-27,
                     bitscore: 91.7, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGASRAERRRRLWEEALVLPPRRDLRESLVGELAGYLGLREDEV
                     AERCEGAAARLAASWRAAAPRTPAQVAAFYRGAGDYLYDLTRWHALVEDESALAAVGAL
                     EEALGRRARRVLDFGSGIGSLGILLARHGLEVTLAEINPSLDAYARWRLARRSLTARHL
                     DLGREPLPRAAFDLIAAVDVLEHLPDPRAALIQLAAALRPGGTLFVHLPPGGDPLHPMH
                     LWDNPETLLEPLEEAGLWLERAEEASLVLRRGPAPRYRLRYGLSLRRAEGGWALFSERP
                     LAAFRLAPEAARLLAVLQEDGRTAAGAAREAGLPLARATALLEGFARRRVLARSGRTPP
                     SRWPSVTVVVPARGRPRETRACVASLLALDYPRDLLEVLVVDDASEPPLARALAGLPVR
                     VLRLERRAGPSAARNLGLREARGEVVAFTDNDCEVSPGWLRALVAPLCEPGVEVVGGRV
                     LSPRPEGALGAFEAVRSPLDMGPSGGRVGLEEAVSYLPSCNLAADRRALLKLGGFDEGM
                     ELGEDVDLVWRAVRAGLGVRYEPSARVVHRHRTRLGAFLRRRADYGSSEADLQLRHPEA
                     GRRMMLPVSGLFWLAALGALARSRRAAVLLAALSLLLAGMETGGKLLRTRRAGALLPLP
                     AVAGAVLRQHAAELYHLGAGVSRYYGLPLLAAALLWRPLRLPALLLLLVPPAVDHRRLR
                     PGLSPAGFALLWGLEMAAYQLGVWRGCLRHRSLRPLLPRLLSPPLFDVLLRILPRESPA
                     PPRSYKLYGRMRKTRFGPQGGERHTQDEA"
     gene            18749..21031
                     /gene="mbhE"
                     /locus_tag="RxyAA322_RS12270"
                     /old_locus_tag="RxyAA322_24490"
     CDS             18749..21031
                     /codon_start=1
                     /gene="mbhE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566060.1"
                     /locus_tag="RxyAA322_RS12270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24490"
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="WP_143528584.1"
                     /transl_table=11
                     /translation="MLGLLSAPAVAAVGAWRPRAAAPAGALFAALSFAAALAGWVLVRG
                     ESVVLPWAPSLGVSFAVELDGLAALYSLLATGIGFAVLVYSAGYLPRHLGHEGRPAAEG
                     GRFYFFMLLFMGAMVGLAMAQDLILIFLFWDLTAIASYFLIGYDAHKEESRYAALMALL
                     VTGITAVLLLVGALLLHGAYGTFYVPGLAERARPGLLLDVAGGLILVAGLAKSAQAPLH
                     FWLPRAMAAPTPVSAYLHSAAMVAAGVLLVGRVYPVVSSSGALLGALLVAGGVSIAVGG
                     LLALTRDTLKQLLACSTISQYGYVVFMYGLGGEYGAAGASFYVIAHALAKSALFLTAGA
                     VTEATGEDRLPELGGLAGRMPLLAASGGAAAAGLAALPLTIGFFKDELLFAAALERGPA
                     CAAIALGAAVLTLAYTWRFWAGIFLGRAGAEPGRVPGTLVWPVTLLGGLVVLGGVLPGA
                     FAGLAGEAAAASLGEPVGGVEVAYHLDARPENLLALATYALGTLAVLTRGGWEPAARLF
                     ARAGEVAGPERGYRLGLRRLNLLSDRAHELEARDLRGRIAGILLPAGVLVGLGLVATPT
                     AGVFRFGEVGADDLPLLIALAAAAAAALATCLTVRHLTLALVVSSAGFSMALVYGFLGA
                     PDVALVAVLVETILTLLLLATMRLIPPGVLREGARVTARQARLKAAVAAVAGLFALGVS
                     WGTFSQPAVQRGAYERLIALTPDAHGKDVVTVTLADFRGLDTLGEITVVALALLGVATL
                     LTRGRLP"
     gene            21028..21480
                     /locus_tag="RxyAA322_RS12275"
                     /old_locus_tag="RxyAA322_24500"
     CDS             21028..21480
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566059.1"
                     /locus_tag="RxyAA322_RS12275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24500"
                     /product="MnhB domain-containing protein"
                     /protein_id="WP_143528585.1"
                     /transl_table=11
                     /translation="MRPEARQGDMTRFVARALLLPALVTAAAILVKGYAQPGDGFSAGV
                     VASLGVLLQYMAFGKEEAERLLPLPPAGLLCFGGLLFALTIAAVPLLLGDPPLTHYPPP
                     GEEPIHLGTLELITAVAFDLGIFLLVFSFSLGVMRLISATREEGEE"
     gene            21477..21872
                     /locus_tag="RxyAA322_RS12280"
                     /old_locus_tag="RxyAA322_24510"
     CDS             21477..21872
                     /EC_number="1.6.5.9"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566058.1"
                     /locus_tag="RxyAA322_RS12280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24510"
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="WP_143528586.1"
                     /transl_table=11
                     /translation="MTFLLSLAVAIIFGAGAYLVLQRNLVRVVTGMILISNAAILFVIS
                     AGLSRGYPPIYPLPEDEPVSDPLVQAMALTAIVISFSVTALLLCMVYRLYTSHGSVDLE
                     DISAAEVREAEALERGESIEKAEEGRP"
     gene            21869..23260
                     /locus_tag="RxyAA322_RS12285"
                     /old_locus_tag="RxyAA322_24520"
     CDS             21869..23260
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566057.1"
                     /locus_tag="RxyAA322_RS12285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24520"
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="WP_143528587.1"
                     /transl_table=11
                     /translation="MILLSLSFGISWVLGAALALADGRRPTAGWLAVAGMGASLVCVGL
                     LTARVLTEGPVWTVAGGWPPGVGITLRADALGAVFALVSTGVVFLALVHEVLAGVRSRT
                     FPALVLLLNAGLCGVFLTGDIFNFYVFFEIAMTAAYALTGYREQDYQIRAAFIFAVVNL
                     IGSVIFLIAVAGMYHLTGSLDMETVARRMAGAEPASTAVISVAVFVAFGLKLGLFPFHF
                     WLPAVYVGSHPPVAAILSGALANIGSYGLLRFGGEILPGFSGLGALVLLALGSASILYG
                     ALQAVSRRLPAEVMAYSAIGQAGYILIAIGVGGTVGYAAATLYSLTNALNKGLLFTATG
                     LRGPWVGASFAVGAFSVAGVPPAAGFLGKLALFRAALEAGGVFWSTVLTSLVFAGGALS
                     FVYCFQVYQRAYMVPDDAKPDGAAQRALLAVLAGLVLLLGLWPEPLAAASSAAASALPA
                     GGGIP"
     gene            23257..23748
                     /locus_tag="RxyAA322_RS12290"
                     /old_locus_tag="RxyAA322_24530"
     CDS             23257..23748
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566056.1"
                     /locus_tag="RxyAA322_RS12290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24530"
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="WP_143528588.1"
                     /transl_table=11
                     /translation="MRRFSVYLVALTLIYAFVLASFHPWDLALGALLSAALLLAFRRFV
                     FVAPPGESGLLRRALYAVPLCLAILHSILKGTWQVALVTLHLRPLSSPGIVAVPVGERT
                     PAGVAFSALATTLAPGTFLVDVDWERGVMLIHTIDASDPEAVRREHEEFYRRYQREVFP
                     "
     gene            23771..24037
                     /locus_tag="RxyAA322_RS12295"
                     /old_locus_tag="RxyAA322_24540"
     CDS             23771..24037
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566055.1"
                     /locus_tag="RxyAA322_RS12295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24540"
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="WP_143528589.1"
                     /transl_table=11
                     /translation="MHEAVFYAASVWMALLLLTSVYAVIRMPSTTGRILALDTLTLVMV
                     GILVLFVDAFRTPYFLDAALILALLAFVGTVAAARYRSERRLF"
     gene            24037..24375
                     /gene="mnhG"
                     /locus_tag="RxyAA322_RS12300"
                     /old_locus_tag="RxyAA322_24550"
     CDS             24037..24375
                     /GO_function="GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mnhG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564770.1"
                     /locus_tag="RxyAA322_RS12300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24550"
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="WP_143528590.1"
                     /transl_table=11
                     /translation="MGALLPWLADALVILGVAVITVGVYGMIRMPDTYTRLHAASKAVF
                     LGVISLLAASVVTGQPAIILRVILIALFLLMTSPVSAHVIGQAAYRRGEEMLSPDAVDE
                     SNRYPPGT"
     gene            24390..25256
                     /locus_tag="RxyAA322_RS12305"
                     /old_locus_tag="RxyAA322_24560"
     CDS             24390..25256
                     /GO_function="GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /locus_tag="RxyAA322_RS12305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24560"
                     /product="universal stress protein"
                     /protein_id="WP_143528591.1"
                     /transl_table=11
                     /translation="MLNRFPQRVLLATDGAAGAALALHAAAEICTRADSELHLVHVWRT
                     LESGPDTELRRQEACWLLRREILAAEAALGGEVKGYLREGMPAEEISALARELEAGLIV
                     MGSRDLGPMERLVMGSVSEGVVNQAPCPVLVVRGGAWPPSRVVVGDDSSEGARRAGELA
                     ARIAVLLGATLQLVRAYPVVLDISEAARLSESIAVPLRVAMSRHEAALLGRARALEGVL
                     GYRPRIRVMEGEAASVILATAEEGDGGPALIAVGRRGLGELDRLRLGSVSTKILRLSRG
                     PVLICPA"
     misc_feature    24576..24578
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(25273..25599)
                     /locus_tag="RxyAA322_RS12310"
                     /old_locus_tag="RxyAA322_24570"
     CDS             complement(25273..25599)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566052.1"
                     /locus_tag="RxyAA322_RS12310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_24570"
                     /product="P-II family nitrogen regulator"
                     /protein_id="WP_143528592.1"
                     /transl_table=11
                     /translation="MRGYKLIAAIVPKGAAGKVMDAAREAGAEGGTILMARGTGVHEAR
                     RVFGITVSSERELVLILVEPEKRDAILDAVVRAGRLDEPARGIAFVMAVEEVKGIVHHE
                     RQED"
ORIGIN
        1 cgccgccctg ctgcacgaca tcgggttgta caaggcctac aacatgcgtg aggcccccga
       61 ccacgccgag cggggggcca tcgtcgccga caggatcctc aaggacgccg acttcccgcc
      121 gcgggcccgg cgggtggtgg tggacgccgt ccggcaccat ccccccgggt cccggcccgg
      181 ctcctccgcc gaggcggcgc tgctcaagga cgccgtcgcc ctggactacc tcggcgcggt
      241 cggcgtctcc cgggtgctgg cgatgatcgg gctcgaggag gaggtcccgg acctcccggc
      301 ggccatagcc catgcccgga gcctgcatcg caccatcccg gagctgctca tcttcgagag
      361 cagcaggatc atcgcccgcg agcgcgccct cgagacggag cgttttctgg aggatctcgg
      421 tcgctcgaca gccgggctga agctcctgta gcctacccgc cgaagtagga ccacagcagg
      481 tatccaccgg cgaccaggca caccgccccc accaccagca tcctgagccc ggctatgacg
      541 tgcacccccc gctccggcag cgggcctatg ccgccgctct gcccccggcc gccgtgcagc
      601 agcgcgtaga gcccgtagcc caccccgccg aagcccgcga ggagcagcag gagccccagc
      661 gccagcgcgg cggacaaggc ggctcagtcc ccctcggaca tccgggagag gagcctccgg
      721 aagcgggggt cctgccggag gggggcgaac tccagcgccc gcttgaagtg ctcggcgtcg
      781 cgctctccgg cctcgatggc cttctccagg tagtccagcg aggcggcgta ctcgccccgc
      841 cgggcgcgga tgctcgccag cgccgagagg gcgggcgagt agcccggatc ggcggccagc
      901 gcccgcacca gcagctcctc ggccgtctcc agctccccgg cggtgtagta gacgttggcg
      961 taggagtaga gcgtccgggg gttgccgggg tcggccttca gcgactccac gaagtgcctc
     1021 tccgccctct cccgctcccc gcggcggtcg tggaggatgc cgagctccag gtgggcctcg
     1081 gggttctccg gatccagccg gaggaccgtc cggtaggcct ctatcgcccc ctcctcgtca
     1141 ccgttcgcca gccgcgcctc gcccagagtg tagaggtgcc ggatctcgtc gctccccagg
     1201 gccacggcct cctcggccag cgagagcgcc tcgcggacgt ctccgccgtg ggccagcagg
     1261 tgggcggcca gctcgtccct tatctcgggg gagccgggac aggcctcgag cccgcgccgg
     1321 aaggcagcct ccgcctcccg caggtgcccg cttctggcgt ggcagatgcc caggttggcg
     1381 tgcgcctccc accactgtgg atcctcggct atcaccgcct cgtaggaggc caccgcctcc
     1441 tcgtagcggc cgagcatgtc cagcgcgttt gcccggaacg tcagcgcccc gaggcgctgc
     1501 tcggcgtcct cggccgcctc gaccgcccgc tcgaagaact ccagcgccgg ggcgtagtcg
     1561 ccggcgtcga agagggtgag cccggcgttg atcagcatgt ccgcgtcgtc cgggtggcgg
     1621 gcgagccacc gctcgaaggt ggcccgggcg agcagcggct cccccatgag cccgtagtag
     1681 cggccgagct ccacgtaggc gtcgggctgc tcggggtcgg cctctatggc ccgctccagg
     1741 tggtggacga tcttgaacgg ctcgtcccgg cgcatcgcca ccatcgccag ccccatccgc
     1801 ggggccaccc acccggggtc catctccagc gcgtcgagaa agctggcctc cgcctcctcc
     1861 agccgtccga gccccaggag cgcccggccc acggcctcga ccacctcggg gttctcgggg
     1921 gcccgctgca gcgcctgctc gaagcgttcc agggcctctt ctatcaggcc ctcgcccgcg
     1981 agcgcctcgc cctgttcgat cagctccctg tggtccatcg agcttaatta aatcagatgc
     2041 ggcaaacgct tacgtgtaaa atctccccct gctgccggcg tgaagtgagg tgatgccttg
     2101 aagaagccac cggtggtgag gcggctcgag atccgctacc aggatctcga cccctacggt
     2161 cacgtgaaca acgccgtcta cctgcagttc ttcgagtcct tgcgcttcgc ctactggcgg
     2221 gcgctcgcgg gggtcgtggg ggccaccgag ttcgaggctg gcgggatcat ccccggcggc
     2281 cggtacgtga tcgccgagac caccgtacgc taccgctcgg agctgctctt cggcgacgag
     2341 ctgcacgggg cggcgagcat ccgcagcgtg ggcaacagct ctttcgtcat ggactacgag
     2401 ctgcgcaagg gagtctcctt cgagggcggt gcgccggtgg ccgacggctc ggccgtccac
     2461 gtcttctacg acatgagatc ccgccgctcc cggccacggc cggactggtt cctgccggcc
     2521 gtggccgagc tggagggccg cccggaggaa ggttttttgc tcgattagaa aggtcgaagc
     2581 aattcttggt ggagaccacg ccttagtggt gatcacgcta atatgtgaag cgtgaaaggc
     2641 gggggtggag gtgcgagatg aatgccacaa gtacagaatg cagccttgta ccgatctcta
     2701 ttacgccgcg ctccgcgtag ttaaaatggc acgctaaagt ctgtcagcgt tatcactgta
     2761 gagggttgcg tcgcctggtt tggcaagacc ccaacacacc gctgtatcag tagacgagtt
     2821 ctggtcgttc gtgagaaaaa gagagccacc tcaagcctca ggggcaagtc ccgaattttc
     2881 tgtaatttcc tccaagatgc gaacctgctc tcatcgctgt gtgagaatca cctcctcctt
     2941 tgctgctcgc tctttcgagc gtcgcttctt gagctgctcc atgtcgagat agcgcctccc
     3001 tgtaacccac tcctccgatt gctcaacggc taaggctgtg accagcctca ggcacgactc
     3061 cctgttaggg aagatcctca ccactctcgt tctgcgcttt atctcctggt tgaacctctc
     3121 cagtccgttg gtggtgcgga tgcgcctgcg gtgggactcg gggaaagcca ggcaagctag
     3181 gcactcctcg acgtgctcct ccaagtgttc ggccaccttt tcgtggctcc ctcgccaccg
     3241 atccgccagc tcctcggcag cccgcaaagc cagctccttc gtgggagcag cgaagatccc
     3301 tcgcaaaccc tcggcaagtt cggcgcgttt gtcgaggccc accatcccca ggaggttctt
     3361 cgcgtagtgg aactggcagc gctggtgact cgctccccga aagtggcgct ctatggcgct
     3421 ctttaggccc ttgtgctcgt cggaggtcac cagttccacc cccttgaggc ctcgctcctt
     3481 tagagcgcga aacagctcct ggtaggtcgc ctcgctctcg acatcggcca cctctaaccc
     3541 gaggatctcc ctatagccgt cgtcgcgaac cgccgaaacg accaagacgc cctcgccttg
     3601 acgatccggc agccaactcg caccttctcg cattaagggc gtccacgaag aggtagggat
     3661 aagcctcggc tgtaagcggc cggctcctcc actctttgag ctccgagtcg agctcggtag
     3721 ccaggcgcga gaccgtgctc ttggagaagg cggtgccgca cagcttctca gtcacctcct
     3781 tgaccttcct ggtggaaacc ccttcgatat acatctccat cagcgccaaa gcgagcgcct
     3841 tctcgttgcg ctctggtagc gggcaaagag ctgggtggag aaggtgcctt cccggtcctg
     3901 gggcaccagc agatcaaggg tgccgactct actcttgagc ttcctcggtt tgtagccgtt
     3961 gcggtagcct ttgcggttct cggtgcgttg gtggggggcg gcaccgagat gctcggtgat
     4021 ctccgcctcc agcatctgct ggagcatccc ttctacgatc ccacgcagga atcggtagtg
     4081 tcccaggaag tgggtagtgt cccaggaagt gtgtagaaaa ggtcgttgac gaagacggcc
     4141 accgtgtggt tcctgaaaga gagtcaatcg aaacaggagg gacacacgat ggccgatgag
     4201 ttgagggtag gactttcgga gttgctgcgc aaggcgcaga tgcaacacga cacggatttc
     4261 cttcgcgaga gcgtcaggac gctcagagaa aggcgctgat ggagttggaa gtggaggagc
     4321 atataggcgc ctcccgccac gagcgcaccg ccgagcggtc gggagtgcgc aacggatacc
     4381 gccacaggca gtgggacacg agggtgggta cggtggaact ttcggttccg agagtacgag
     4441 acggcagcta cttcccctcg cttttggagc cgcgcagaaa ggctgagagg gcgctctccg
     4501 ccgtggtcca ggaagcctac gtgcatggcg tctctacccg caaggtggac gagctggtgc
     4561 aggctcttgg catgagcggc gtctccaaga gccaggtcag taggctcttg gagcaactcg
     4621 atgaggaggt cgaacgcttc cgcagccggc cgcttgaagg ctcctacccc tacgtgtggc
     4681 tggacgccac ctacgtaaag gcgcgccagg acggacaggt agtgagcact gcggtggtga
     4741 tcgccatcgg tctcaacgcc gagagcggcc agagggaggt tctctggggc ttgatgtggg
     4801 tcccagtgag gacggagcat tctggctctc tttcctacgc tctttggtgg cccgtgggct
     4861 ctcgggagta aggttggtaa ccagcgattc ccaccaagac ctcaaaggcg ccatagaagc
     4921 cgtattgcag ggagcgggct ggcagaggtg tcgagttcat ttcatgagga acgccctttc
     4981 cacggttccc aaagaagctt taagcagatg gtcggggcca cgatccgcac cgtctttgcc
     5041 cagcccgact ctgagagcgc ccatcagcaa tggcgtaggg tagccgacgg tttcaggggc
     5101 cgttttgcga agctggcgca tctgatggac gaggccgagg aagaggtact ttcctacgcg
     5161 acgtttccca aagagcactg gcagaagatc tggtcgaaca accccctgga gaggttgaac
     5221 aaggaggtca agaggaggac agaagtggtg ggcatcttcc ccaacgaggc agccgtcgtc
     5281 cggctggtgg gagctatctt gaacgagcag catgatgaat ggcaggtagg aaagcgttac
     5341 tttggcgcgg gatcgctgga gaagctgggc gagcagagtg gcgaagcgca ggctgctcat
     5401 gagcagcctg cgctgatagg aggctgaggc agagttggaa atgaggccac acggtgtgaa
     5461 ttacacactt gacgggacgc ataccaggaa tcccggatcg tcgagcaacg ccccttgcac
     5521 cagctcttcg tcgagcctac gatgacgttt ggccatcgtc tcacctctat cgatcggttg
     5581 acctttgaca tcaaccatct tggaggacga tggtccttct tttccagtgg ctcaccaatt
     5641 tacagaagtt tagggacaca atcggctgac acgcctgacg cgcaagatgc acaccttcgc
     5701 taagagcgct tcaacgtgga atacgctgtt cgggctggcg ctcttcgagc gcaactggct
     5761 gtggacgcac ctggcgctta gccagccgag taccgcaccc aggcgacgct aagcggcgca
     5821 tgccatcaga tggcctagga ttggccgacc atcgctggtc ttggccagag ttcctgacca
     5881 cgagggctat attgtcaact cttgaaggca gcactaccaa aactattgac tagtaccctg
     5941 ctgtaaccgt ataattatat ttgaataaga cccactcggg tcgaagagga gatctgtagg
     6001 ctgaggtgaa gggaaggagg agtagtagtg tcccagagga cagaaacggc tgagcgctgg
     6061 gtggaagatt tccggcgtgt cactaacgaa gatccggaga tccaagccca tggaaaatac
     6121 ttttcgtgct cttttcttct ggacatggag cagagcaggt tcttggtgaa gatgcacgaa
     6181 ggcaaggtcg aggagatctt gacgaatcca ggacctctgg atgagcgcta ccagtttgct
     6241 cttagggcga gtgcggaaac ttggcgcaag ttcggagaag aagttccgga gcccatgtat
     6301 catggtattt ggtcagcttc ttttcaaaga gatctgcgct tggaaggaga tctcttggta
     6361 ttgatgcaga acttgcgttg cttcactcgg caattcgagc ttcttcggca aatcggtgtg
     6421 ccggtatagg ggcgtacttg aagggaagag gagagtagat ccatggcgca gttttcgccg
     6481 gttactggga gatatgtaac cattgacgta ctcggggacg agtgtaaggt tttcttcttg
     6541 gagaatggag aggggcagcc gctcgtctgt cagcatactg cgggctgcca caatcatcag
     6601 tggcgagatc ttttggagga cgaggaaatc actcgcaact atagggtaat tgcctatgac
     6661 cttgctcgcc acggaaagtc cgatcctccc aagaacaggg agtggtggaa agaagagtat
     6721 cggcttacag ccgattatta cactgccttt atagtagcgt tctgtgatgc gctagagctt
     6781 gaggatccta tttttatggg atcttcgttt ggaggtaacg ttgctttgca gctggccttg
     6841 aggcatccgg accgcttcaa ggcagtaata ccggttgaag ctgcggaata ttccccgggt
     6901 ttcttcctcg attggtggca gcatccacaa gccaacgccg cacaggtttg tgccagtgga
     6961 gtttgggatc tgatggctcc ccagtcgccc gaaaaagatc gttgggcaac atggttctat
     7021 tactctcaag gttccgaagc ttttcgtggg gacctttatt tctactcggt agatcatgat
     7081 cttcgaggga aactggatca gatagatggt gagaagtgtc ctgtaatcat gctcacgggc
     7141 gattatgatt acctcactac tcccgaggac agtcagcgca ctgcggatca gataaagggc
     7201 ggagagttca tagagatgaa gggaataggt catttcccaa tgagcgaaaa ttatccgctt
     7261 tttaagcgct accttcagca ggctcttcag aggattgaga accgcactgg aggttgagag
     7321 taggcgcaaa ttgttaacct gatcaaagat atggctctct cctgcgacga atgagttaga
     7381 ggacggaacg tcgtaggaga gggccatctt tgtcaggttc ccggttcgga gctggtagtg
     7441 aacgaggcga acttctcgcc atggctggtg gggataatgc tctcgggggc tttggcggct
     7501 gcgatgtcta ccggatcgaa cctggcccat accgcctcga ccgtgctggt gagggacctg
     7561 ttcgtggcgg tgttcaggca agatatgccg gagaggcagg tggtcctgct caccaagatc
     7621 ttcgtcgtcg tgatctcggt ggtcgcctac gtgctggcgc tcttcaaccc ggcttccctc
     7681 gttgggcttt tgctcggcgc gtacggggcg gtggtgcagt tcttcccact gatcgtggcc
     7741 gtcttcttct ggaagcgggc caccaaagcg ggcgcgttcg ccggtctgat ctccggtagc
     7801 gcggtcatgc tctacttctc gtttctcgct cccccgccct ttgagataca cgccgggatc
     7861 tgggggcttc tggccaacac ggtagcgctg gttgccgtga gcctcctcac ggagcccatg
     7921 cccgaggagc acgtcgagag gtttgtggag ggctccaagg cttcgctgga ggagatctcc
     7981 ggccccccgc ggccagacgc ctcgaccgca tagaaaagga taagccccaa ccccggcgga
     8041 cccgggctcg cctaaaatac cggcgtggag ctgagggatc tcgaagcttt cggcgagccg
     8101 ggggcgatgg tctacctggc ggattgcgtg gagctcatgc gccttatgcc gccagggagc
     8161 gtggacgcca tcttcgccga tcctccctac cggctctcgg gcggcggcgt gacggtgagg
     8221 gccggacggc tcgtccccgt ggacaagggc gggtgggacc ggccgctcgg gcctcggggc
     8281 aaccacgagt tcaacctccg ctggctgcgg gaagcgcgcc gcattctgaa gccgggcggg
     8341 agcctctggg tctccggcac ccaccacatc atcttcagcc tcggtttcgc gctccagacg
     8401 ctgggcttcc gcatcataaa cgacatcgtc tggcagaagc ccgacccgcc cccaaacgcc
     8461 ctgcgcacgg cgttcacgca cgcgcacgag accctgatct gggcgagcaa ggggcgcggg
     8521 cacaccttca accacgaact gctggacggc ccggcccccg cctcgcggat ctcctcggtc
     8581 tggcgcatgc cgcctccccc ctccagggag cggctgcacg gttaccatcc cacccagaag
     8641 cccctgcgcc tggtgcgccg ggcgcttctg gcctcgacgc gggaggggga gctggtcttc
     8701 gaccccttca ccggctccgg gaccacggcc gtggccgcga aggagctggg ccgcttcttc
     8761 gtcggtgccg agctggagag ggagtacgcc gagctggccg gccgccggat aagggccgcc
     8821 gggcgcggca ctgcagtcca gccggcgcgg ggctcgggga accccgccgg ttgaggagaa
     8881 agccagaagg ggagtatcat gttgcggcat ccctttcacg gtgcacggga aggaggaggg
     8941 agaatggacg tcacccgcga gagcacttct gccgccgcga cccccgtccg ggaggacgac
     9001 cggccggaag aagccgcccg gaccggcggg ggcgagtccg gtgccgtcga ggaggagatc
     9061 ctcatcgagg aggtcagcat agacggcatg tgcggggtct attaggtcct cgcgcccttg
     9121 gagggacggc ggaccctgct cctggaggac agggtggccc tcatcacggg ggcggcgcgc
     9181 ggccaggggc gctcgcacgc gctcaccctg gcccgggagg gagcggacat aatagcctgc
     9241 gatatctgcg agccgatctc cagcgcccct tatcccatgg gtagccgaga ggagctggag
     9301 cagacggccc ggatggtcgc ggagctcggc aggcgggtcg tcgcgcagaa ggccgacgtg
     9361 cgcagcctgg agcagctgcg cgcggtggtg gagcgggggc tctccgagct cgggcgcata
     9421 gacgtcgtgt gcgccaacgc cggcgtgatc agctacgccc cctcctggcg gataaccgag
     9481 gagcagtggc gcgagacgct ggagaccaac ctcaccggcg cctggcacac ctgcagggcc
     9541 gtcatccccc acatgatgga gcggggagag ggcggctcca tcatcctcac cggctccacg
     9601 gcggcggtgg tgggcttcga aaacctggcc cactacgtct ccgccaagca cgggctggtg
     9661 gggctcatga agacgctggc caacgagctg gccccccacc gcatccgggt gaacatcgtc
     9721 caccccacca gcgtcgacac cgccatgatc cacaacgagg ccacctaccg cctcttcgtc
     9781 cccggcgcgg accgcacccc cacccgcgag gaggccgccc gcgccttccg caccctcaac
     9841 gccctgcccg tcccctgggt ggagccggag gacgtctcga acgccgtgct ctggctcgcc
     9901 tcggaccact cccgctacgt caccggccag cagctgcggg tggacgccgg ggccgcggag
     9961 aaggcgagga ggtagaaaag aaaggaggca gaaaaccgct ttgacgctca gagacgaggc
    10021 ccggtgcagc cttgcgccgc aggtgtccgt gagggccgag cgattcggtg gtctaatcta
    10081 ccgctacgac gaccgggcgc tgctctttct gcgctcccgg ccgctggtgg agctcctgct
    10141 cgggctggac ggcacgaaga cgctcaagga ggcgctggag gagctcgccg ccgagcggga
    10201 cctcggcgtc cgcgagctgc gcgccgtaaa ggacgctctg cagaagctcg aagagagggg
    10261 gatagtccgt gagctatgag ctcttgagag aggggcttga cgcccccatc tgcctcacct
    10321 gggagctcac ctaccactgc aacctgcgct gcgcccactg cctctccgac tccgggatgg
    10381 ggcgcgagga cgagctcacc accggggagg ccaggtccct catcgacgag tgggccgaga
    10441 tgaaggtctt ctacgtaaac gtcggcggcg gggagcccat gacccgcccg gacttcttcg
    10501 agctcatgga ctactgcctc cagaggggca tcggggtgaa gttctccacc aacggcaccc
    10561 tcctcgacga cgcggcggcg gactggatct cctcccgccc ctacctggac gtgcagataa
    10621 gcctcgacgg ggccaacccc gagaccaacg accccgtccg cggtaccggc tccttcaagc
    10681 gggcccggcg cgccatggag cggctcgccg agcgctcctt ccccttcaag ataaacaccg
    10741 tcctcacccg ccacaacttc ccccagctcg acgagctcta cgcgctggcg aaggggtacg
    10801 gggcccggct gcgcctcacg cgcctcagac cctccggccg gggcgtgcgg gtgtgggggg
    10861 agctgcgccc cacccgcgag cagaacagag agctctaccg ctggctgcac gcccaccccg
    10921 acgtgctcac cggcgactcc ttcttccacc tctccgccta cggagagccg ctcgcgggga
    10981 tgaacatgtg cggggccggg cgcatcgtgt gctgcgtgga ccccgtgggg gaggtctacg
    11041 cctgcccctt tgtgctcgcc cccgagttct cggcgggcaa cgtgcgcgac cccggcggct
    11101 tcgccagggt gtggcgcgaa tccccgctct tcaggcgcct gcgggagtgg caggtgggcg
    11161 gtgagtgccg gagctgcaac gcctaccacc tgtgccacgg gggctgcatg gccgtaaagc
    11221 acttcacagg gcgcccgctg gactcccccg accccgactg cgtcttcggc cggggagagg
    11281 agccttcccg ggggcagttc gtggccctgg ggtccggaaa gaggggcgcg ggggcggcgt
    11341 cggcgctaga atgatgttcc ccacaaccgc gggtgattcg ggaaaggagg agcggtgaag
    11401 agtcgtgcgg ctttgctcta cggcgagggg caggagttca gggtcgagga gatcgaggtg
    11461 gacgacccca agcagaacga ggtgctggtg cacctcgcgg ccacggggct gtgccactcg
    11521 gactaccaca tggtgacggg tgagtacgga ccgctgcact tccccatgat cgggggtcac
    11581 gagggcgccg gggtcgtgga gaaggtggga cccggcgtca cccgggtgaa gcctggggac
    11641 cacgtcctgc tcaccttcat ccccgcctgc ggccactgcc ggttctgctc catgggcctc
    11701 agcttcctgt gcgaccgggg tgcggcgatc ctgcaggggc cgcagctcga cggcaccttc
    11761 cggatgcaca ccgccgacgg gcaggacgcc gggcagttct gctgcatctc caccttcagc
    11821 gagtacaccg tggtccccga ggcttccatc gtccctatcg ccgatgacct cgacctgacc
    11881 aaggtctgca tcgtgggctg ttgcgtgccc accgggttcg gctcggcggt caaccgggcc
    11941 gacgtccagc cgggcgagac ggtcgtgatc ttcgggatcg gcggggtcgg catcaacgcg
    12001 gtgcaggggg cggcggtgaa gggcgcctcc aaggtggttg ccgtcgaccc ggtggacttc
    12061 aagctggaga ccgccgaaga gctcggcgcc acccacacca taaaccccaa cgagcgggac
    12121 cccgtccaga ccgtcgtcga cctcaccaac ggggtcgggg cggacaaggc gatcatcacc
    12181 atagacaacg tgctgcccga gcacgtcggg caggccttca gggccgtccg caagggcggc
    12241 cgggtggtgg tgaccggggt ggcccgctac gaccaccagc acatagacgt ctcgccctac
    12301 gagctgaccc tcttcgccaa ggaggtggtc ggcaccatgt acggtcactc gccggtcaac
    12361 tccgacctgc agcgctacct ggagctctac cgcacgggcc ggctcaaggt ggacgagctt
    12421 atcacccgca cctactccca cgacgagatc aaccagggct accgcgacat gctcgacggg
    12481 aagaacatcc gcggcgtgat cgtctacgac tgggcgaggt gagcccgcgg cggtggacct
    12541 gatccgcatc cacgacgagg tccggcgcag catcgtcggc cgcgaggagg agctgcagct
    12601 cctcctcgcg gccctcgccg cgggccgcga cgtgctcctg gaggggcctc cggggacgag
    12661 caagtccacc ctcctgcgcg ccgtgacgcg tgcgagcggt tccagcctgc gcttcgtaga
    12721 gggcaacgcg gacctgaccc cggcgaagct ggtcggtcac cacagcccct cccgggtgct
    12781 gcaggaggac tacgggccgg agaacttcgt ctacggccct ctcccccagg cgatgcgcga
    12841 gggcgggatc ctgtacatcg aggagctcaa ccgggtaccc gaggacaccc tgaacgccct
    12901 gctcaccgcg atggccgagc gcgagctcac catcccccgc gtcggggtgg tgcgcgcggc
    12961 ggagggcttc cgggtcgtcg cggcgatgaa ccccttcgac aacatcggca ccgcccggct
    13021 gggcggggcc gtcaccgacc gcctgtgccg ggtccggctg gactaccagc cggaggacga
    13081 ggagcgccgg atagtggccc gcaggaccgg gagcgaggac gccttcctgg tgggcctcgc
    13141 cgtccgggtc gcccgcctca cccgctcgca ccccgacgtc cggatggggg cctcggtgcg
    13201 cgccgctata gacttcgtgc tggtggccga acagctgctg cggctgcgcg ggcttgccgc
    13261 ccccgacgcc ccccgcgacg aggcggtccg gaggaccctc gtctccgccg cccagacggc
    13321 cctctccatc aagatcgccg tgcgcgaagc ctccgggcgc tccgccgacg aggtggtcga
    13381 ggaggtggtg cgcgccgccc tcgccggccc ccgggacccg gaagagcccc cggagccctc
    13441 ctccaacggt gcgccggagg gtgtcccgcg ggctccgcga ccccgttccg gcggggagac
    13501 ccgccggacg gtggggcagg gatcgctcgg ctcgctggcc aggagccggc cgaatctcgc
    13561 cgggaggctc gccgcggagc gtagcctcga cgccgccgcc ctggaagcgg cggtccggga
    13621 cgaggggggc gagccgctcg aggttcttgg cgagctggtc gacctctacg accgggcgga
    13681 cctgcgcgag ctcgcccgga ggctggcgcg cgagctcgtc ctgcgggcgg cccgccgcga
    13741 cgccgccggg aggggcggca ggggtcgcct tgcgagctcc ccctaccggg ggcaggccgc
    13801 ggagctggac ctggaccgct ccctggagcg ggcgctcctt cccccggccc cccgggacga
    13861 agagctcttc gtgctcgacc ggcgccacca ccggcgcgcc tacgcgctgg tgctcgacgc
    13921 ctcgggctcc atgaagggcg gggctctcct gcgcgcagcc ctcgccgcct cggccttcgc
    13981 ggtgcgcgcc gacccggacc ccttcgccgt cgtcgccttc tggcgggagg tggccgtgat
    14041 caaacacctt gaggagcggc tccctttgga ggtcctcctc gaccgggtgc tctcgctgcc
    14101 cgcccggggc ctgaccgacc tctgcctagg cctgcggacc gggctagagg agctggaagg
    14161 gagcggcctg cgggaacgca ccgggcttct gttcagcgac gggatgcaga ccaccggctc
    14221 gcccgcagag cccgtcgccg cggccttccc tcaacttcac gtggtggccg ccgcccagag
    14281 tccggagtcg ctcgcccggt gccggcacct ggccgatctc gggcggggcc ggtacgcccc
    14341 ggtaaggagc atctcggaga taccggccgc gatcaacagc tgtctggcgg cgtaggaggg
    14401 gagagatggc gagcagcggg gagtggttcg agtcggtcgc cgaggcacag cggagggccc
    14461 ggcggcggct gcccagatcc gtgtacaggg cgcttctcgc gggcaccgag aagggcctca
    14521 ccctccggga caacctggcg gccttcgacg agctgggact ggtgccccgt ctcgtggggt
    14581 tcccgggcgg aggcttcgag ctctccacgc gggtgatggg cctggaggtc tcgatgcccg
    14641 ttctcatctc ccccaccggg gtccaggccg tccaccccga gggggagctc gcggtggccc
    14701 gggccgccgc cgcccggggg acggccatgg gcctcagctc cttcgccagc aagccggtcg
    14761 aggaggtcgt ctccgccaac cccaggacct tcttccagac ctactggatc ggaggcaggg
    14821 agcggatgct cgggatcgtg gagcgggccc gggaggcggg ggccgccggc atgatcctca
    14881 ccctcgactg gagcttctcc cacagccggg actggggcag cccctacatc ccggacagaa
    14941 tagacctcaa gacgatgctc cgcttcgccc cggaggtcgc cctcaaaccc ggctggctgc
    15001 tgaggtacgc gaacgcgcgc tccctccccg agctcaccgt gcccaacatg acgccttctc
    15061 ccaaggaccc ggcccccacc ttcttcgggg cctacgccga gtggatgcag tccccaccgc
    15121 ccacctggga ggacgtggcg tggctgcggg agcggtgggg cgggcctttc atgctcaagg
    15181 gcgtgatgcg cgtcgacgag gcccgcaggg ccgtggagga ggcgggggtg acggccatct
    15241 ccgtctccaa ccacggcggc aacaacctcg acggcgtccc ggcctccatc cgggcgctgc
    15301 cggccatcgc cgaggcggtg ggcgacaggg tggaggtgct gctcgacggc ggcatccggc
    15361 gtggcggcga cgtcgtcaag gcgctggcgc tgggcgcccg ggccgtcatg atcgggcggg
    15421 cctatctgtg ggggctcgca gcgggcggac aggccggggt cgagaacgtg ctggagatcc
    15481 tgcgttccgg gatggagtcc accctgcgag gtctcggaaa gagttccatc cacgacctgg
    15541 acagagacga cctgatcgtg ccggcgggct tcgagcgccg gctcggcgag ggggatgggg
    15601 aataggctcg ccgagatgag ctggccggag gtggagcggg cggtggccgc cggggccacc
    15661 acggtcctcc tgccgctcgg ggccaccgaa cagcacggac cccacctgcc gctcggaacc
    15721 gacaccgtga gggccgaggc gctggccggg ctgctggccg agaggcttcc gggcgcgctc
    15781 gtggcgccgg cgcttccttt cggctgctcc gacgagcacg ccggctttcc cgggcttctg
    15841 ggggtcggga aggagacgct ggcccgcctg atcgtggacc tcgcccgcag gatggccggg
    15901 tggggggtgg agcggctggt gctgctctcg gcgcacgggg gcaacgggga ggcgctctcc
    15961 ctggcccgcg cgatgctccg gagcgagctc cccggcctcg ccgtctgggc gccggaggac
    16021 ctcacctccc tctccccggc caccgccggg gtggcccgcc gcgcgggggt gcccgaaggc
    16081 gcgctcgggc tgcacgccgg ggagggtgag acctcggagc tgctccacct gcgcccggag
    16141 ctcgtgcgcc gggagagggc ccgccccggc tacaccgggg acatggaatc cgtcgtaaag
    16201 atcctgaagg ccggcgggct gcgggcggcc caccggagcg gggtgctcgg ggatccgacc
    16261 ctcgcctcgg ccggtcgggg cgcctcctac ctggaggcgc gggcggagga gattgcccgg
    16321 gccgtgctcg gcgacccgga tggcgggcgc tagccgcgcg gagcgccgga ggcgcctgtg
    16381 ggaggaggcg ctggtgcttc ctccccggcg cgacctgcgg gagagcctgg tgggcgagct
    16441 cgccggctac ctggggctcc gtgaagatga ggtggccgaa cgctgcgagg gtgcggccgc
    16501 ccggctcgcg gcgagctggc gtgccgccgc gccccggacg cccgcgcagg tcgcggcctt
    16561 ctaccggggg gccggggact acctctacga cctcacccgg tggcacgcgc tggtggagga
    16621 cgagagcgcg ctcgccgcgg tgggggctct ggaggaggcc ctcgggcgcc gggcccgcag
    16681 ggtcctggac ttcggcagcg gcatcggctc gctcggcatc ctcctcgccc gccacgggct
    16741 ggaggtgacc ctcgcggaga taaacccctc gctcgacgcc tacgcccgct ggcgcctggc
    16801 ccggcgcagc ctcaccgccc ggcatctgga cctgggccgc gagccgcttc cccgggctgc
    16861 cttcgacctg atcgcggcgg tggacgtgct cgagcacctc cccgatccgc gcgccgccct
    16921 gatccagctc gccgcggcgc tcaggcccgg ggggaccctc ttcgtccacc tgcccccggg
    16981 cggggacccg ctgcacccga tgcacctgtg ggacaaccct gaaaccctcc tcgagcccct
    17041 ggaagaggcc gggctgtggc tcgagcgggc ggaagaagcc tcgctcgtcc tccggcgcgg
    17101 gcccgcccca cgctaccggc tgcggtacgg gctctccctc cggcgggctg agggggggtg
    17161 ggccctgttc tcggagcgcc ccctcgccgc cttccgtctc gccccggagg ccgcccggct
    17221 cctcgcggtt ctccaggagg acgggcggac ggcggccggc gcggcccggg aggccggact
    17281 gccgctcgcc cgggccacgg ccctcctgga gggcttcgcc cggcggcggg tgctggcgcg
    17341 ctccggccgg acgcctccct cccggtggcc ctccgtcacc gtcgtcgtcc cggcccgggg
    17401 ccggccccgc gagacccggg cctgcgttgc gtcgctgctc gccctcgact atccccggga
    17461 tcttctggag gttctggtcg tggacgacgc ctcggagccg cctctcgccc gggcgctcgc
    17521 cgggcttccg gtgcgggtgc tccggctgga gaggagggca gggccgtccg ccgcacgcaa
    17581 cctggggctc cgggaggcgc ggggcgaggt cgtggccttc acggacaacg actgcgaggt
    17641 ttcgcccggc tggctgcgag ccctcgtggc gccgctgtgc gagcccgggg tcgaggtggt
    17701 gggggggagg gtcctgtccc cgcgcccgga gggggcgctc ggggccttcg aggccgtccg
    17761 ttcgccgctc gacatggggc cctccggcgg gagggtcggt ctggaggagg cggtgtccta
    17821 cctgccctcc tgcaacctcg cggcggaccg gcgggccctg ctgaagctgg gcggcttcga
    17881 cgagggtatg gagctcgggg aggacgtgga tctcgtgtgg cgggcggtgc gggccgggct
    17941 gggggtgcgc tacgagccct ccgcccgggt cgtgcaccgg caccgcaccc gcctcggggc
    18001 cttcctgcgg cgccgggcgg actacggctc ctcggaggcc gacctgcagc tgcggcaccc
    18061 ggaggccggc aggaggatga tgctcccggt ctccggtctc ttctggctcg cggcgctcgg
    18121 agcgctcgcc cgctcgcggc gagccgcagt cctcctcgcc gcgctctccc tcctcctcgc
    18181 ggggatggag accgggggca agctgctgcg gacacgacga gccggcgcgc tgctcccact
    18241 cccggcggtc gctggtgcgg tgctgcgcca gcacgcggcg gagctctacc acctcggcgc
    18301 gggcgtctcc cgctactacg gcctgccgct ccttgcggcc gcgctccttt ggcgaccgct
    18361 ccggttgccg gcgctcctcc tgctgctcgt cccgccggcc gtggatcatc ggcggctgcg
    18421 tcccggtctc tcgccggccg ggttcgcgct gctctggggg ctcgagatgg ccgcctacca
    18481 gctcggcgtg tggcgcggtt gcctgcggca tcgcagcctg cggcccctcc tgccgcgcct
    18541 cctctcgccc ccgctgttcg atgtgctcct gcggattctc ccacgcgaat ccccggcgcc
    18601 tccccgctcg tataagctgt atggcaggat gagaaagacg agattcggtc cccaaggggg
    18661 ggagcgtcat acgcaggacg aagcatgatg taacaacccg tgctcccgga ggtgcgtcct
    18721 gagggaggtt ctggtcctcg cgccgctggt gctggggctg ctctcggcac ccgccgtcgc
    18781 ggccgtgggg gcgtggcggc cgcgggctgc ggccccggcc ggggcgctct tcgcagccct
    18841 ctccttcgcg gcggcgctcg cggggtgggt tctcgtccgg ggagagagcg tcgtcctccc
    18901 ctgggcgccc agcctcggcg tgagcttcgc ggtcgagctg gacgggctcg ccgcgctcta
    18961 ctccctgctc gccaccggca tcgggttcgc cgtgctcgtc tactccgcgg gctacctccc
    19021 gcggcatctg gggcacgagg ggcgtcccgc cgccgagggc ggccgcttct acttcttcat
    19081 gctgctcttt atgggcgcga tggtgggcct ggccatggcc caggacctca tcctcatatt
    19141 cctcttctgg gacctcaccg ccatcgcctc ctacttcctc atcgggtatg acgcccacaa
    19201 agaagagtcg cgctacgccg cgctgatggc gctgcttgtc accgggatca cggcggtgct
    19261 gctgctggtg ggggcgctgc tcctgcacgg agcctacggg accttctacg tccccgggct
    19321 cgcggagcgt gcccggcccg gcctcctgct cgacgtggcg ggagggctta tcctggtggc
    19381 cgggctcgca aagagcgccc aggcacccct ccacttctgg cttccgcggg cgatggccgc
    19441 ccccacgccg gtctcggcct acctgcactc ggcggccatg gtcgccgccg gggtgctgct
    19501 cgtcgggcgg gtctaccccg tcgtctcctc gagcggggcg ctgctcggcg cgctcctcgt
    19561 cgcggggggc gtctccatcg ccgtgggcgg gctgctggcc ctcacccggg atacgctcaa
    19621 gcagcttctg gcctgctcca ccatctcgca gtacgggtat gtggtcttta tgtacgggct
    19681 cggcggggag tacggggccg ccggggcctc cttctacgtc atcgcccacg ctctggccaa
    19741 gagcgccctg ttcctgaccg ccggggcggt aaccgaggcc accggtgagg accggctccc
    19801 ggagctcggg gggctcgccg ggaggatgcc gctgctcgcc gcctcgggcg gggcggcggc
    19861 cgccgggctg gccgccctcc cgctcaccat cgggttcttc aaggacgagc tgctgttcgc
    19921 cgcggcgctg gagaggggac ccgcctgcgc ggccatcgcg ctcggtgcgg cggtgctcac
    19981 gctggcctac acctggaggt tctgggccgg gatcttcctc ggccgggccg gggcggagcc
    20041 ggggcgggtg ccggggacgc ttgtgtggcc ggtgaccctt ctcggggggc tggtcgtcct
    20101 cggcggcgtc ctgccggggg ccttcgccgg tcttgcgggg gaagcggcgg cggcctccct
    20161 cggggagccg gtgggcgggg tggaggtggc ctaccacctc gacgcgaggc cggagaacct
    20221 gctcgcgctc gcaacctacg ccctcgggac cctcgccgtt ctcacccgcg ggggatggga
    20281 gcccgccgcg cgcctcttcg cccgcgccgg ggaggtggcc gggcccgagc gcggctaccg
    20341 gctcgggctg cggcggctga acctgctctc cgaccgcgca cacgagctcg aggccaggga
    20401 cctcaggggc aggatcgcgg ggatcctcct tcccgccggg gtgctcgtcg ggctggggct
    20461 cgtggcgacg cccaccgcag gcgtcttccg cttcggggag gtgggggccg acgacctgcc
    20521 gctgctcatc gccctcgcgg ccgctgcggc cgccgcgctc gccacctgcc tcaccgtccg
    20581 gcacctcacg ctcgcgctcg tcgtctccag cgcgggcttc tccatggcgc tcgtctacgg
    20641 gtttctgggt gcgccggacg tcgcgctggt cgccgtgctc gtggagacca tcctcacgct
    20701 gcttttgctc gccaccatgc gcctgatccc gcccggggtc ctgagggagg gagctagggt
    20761 gaccgcccgg caggcccgcc tgaaggcggc ggtcgccgcg gtggccggtc tgttcgcgct
    20821 cggcgtctcc tggggcacct tctcccagcc cgccgtccag cggggagcct acgagcgcct
    20881 catcgcgctc acccccgatg cccacgggaa agacgtggtc accgtcaccc tcgccgactt
    20941 ccgggggctc gacacgctgg gcgagatcac cgtggtcgcc ctggccctgc tcggggtcgc
    21001 caccctcctc acccggggga ggctcccgtg aggccggagg cccgccaggg cgacatgacg
    21061 cgctttgtcg cccgggccct gctgttgccc gccctcgtga cggccgctgc catcctcgtc
    21121 aaggggtacg cccagcccgg ggacggcttc tcggcggggg tggttgcctc cctgggggtg
    21181 ctgctgcagt acatggcctt cggcaaggaa gaggccgagc gcctcctccc gctgcccccg
    21241 gccgggctcc tgtgcttcgg gggtctcctc ttcgccctca ccatagccgc cgtcccgctc
    21301 ctgctcgggg acccgccgct cacccactac ccgccccccg gcgaggagcc catccacctc
    21361 gggaccctgg agctcatcac cgcggtggcc ttcgatctcg gcatcttcct gctcgtcttc
    21421 agcttctccc tgggggtgat gcgcctgatc tcggcgacgc gggaggaggg cgaggaatga
    21481 ccttcctcct ctccctcgcc gtcgccatca tcttcggagc cggggcctac ctcgtcctgc
    21541 agcgcaacct cgtgcgggtc gtcaccggca tgatcctcat ctccaacgcc gcgatactct
    21601 tcgtgatctc cgccgggctc tcgcgcggct accctccgat ctacccgctc ccggaggatg
    21661 agccggtcag cgacccgctc gtgcaggcga tggccctgac cgccatcgtg atcagcttca
    21721 gcgtcaccgc gctgctgctg tgcatggtct accggctcta cacctcccac ggctcggtgg
    21781 atctggagga tatctcggcg gccgaggtgc gggaggccga ggcgctcgag cggggggaga
    21841 gcatcgagaa ggccgaggag gggaggccgt gatcctgctc tccctctcct tcggcatctc
    21901 ctgggtgctc ggggctgcgc tcgccctcgc cgacgggcgc cgcccgaccg ccggctggct
    21961 ggccgtggcg gggatgggag cctccctggt gtgtgttggg ctgctgaccg cccgggtgct
    22021 gacggagggg ccggtgtgga cggtggcggg agggtggccc ccgggggtcg gcatcacgct
    22081 gcgggccgac gccctcgggg cggtcttcgc gctggtctcc accggggtcg tctttcttgc
    22141 gctggtgcac gaggtgctgg cgggggtgcg ctcacggacc ttccctgccc tcgtgctgct
    22201 gctcaacgcc ggcctctgcg gcgtcttcct caccggggac atcttcaact tctacgtgtt
    22261 cttcgagata gccatgaccg ccgcctacgc gctcacaggc taccgggagc aggactacca
    22321 gatccgggcg gcctttatct tcgcggtggt caacctgatc ggctcggtga tcttcctaat
    22381 cgccgtcgcc ggcatgtacc acctgaccgg gagcctggac atggagacgg ttgcccggcg
    22441 gatggccggc gccgaaccgg cctcgacggc ggtgatctcg gtggcagtct tcgtggcctt
    22501 cgggctgaag ctggggctct tcccgttcca cttctggctc ccggcggtct acgtgggcag
    22561 ccacccaccg gtggccgcca tcctctcggg ggcgctggcg aacatcggca gctacggcct
    22621 cctgcgcttc ggcggggaga tcctgccggg cttctccggg ctgggggcgc tggtcctgct
    22681 ggccctgggc agcgccagca tcctctacgg ggccctgcag gccgtctcgc gccgcctgcc
    22741 ggccgaggtg atggcctact cggccatcgg gcaggcgggg tacatcctca tcgccatagg
    22801 cgtcgggggg accgtcgggt acgccgcggc aaccctctac tccctgacga acgccctgaa
    22861 caagggactt ctgttcaccg ccaccgggct gcggggaccg tgggtggggg catccttcgc
    22921 ggtgggcgcg ttctccgtcg ccggggtgcc gccggccgcc ggctttctgg ggaagctcgc
    22981 gctcttccgg gccgcgctcg aggccggagg cgtgttctgg agcacggtcc ttacctccct
    23041 ggtcttcgcc gggggtgcgc tctccttcgt ctactgcttc caggtctacc agcgggcgta
    23101 catggttccg gacgatgcga agcccgacgg ggctgcacag cgggcgctgc tggcggtgct
    23161 cgccgggctg gtgctcctgc tcgggctctg gccggagccg ctcgccgcgg ccagcagcgc
    23221 ggcggcctct gccctgccgg cgggaggggg catcccgtga ggcgcttttc ggtctacctc
    23281 gtcgcgctca ccctgatcta cgccttcgtg ctcgcgagct tccatccctg ggacctcgcc
    23341 ctgggggccc tgctgtccgc ggccctgctg ctggccttca ggcgctttgt cttcgtcgcg
    23401 ccccccgggg agtcagggct gctgcggcgg gcgctgtacg cggtgccgct ctgcctcgcc
    23461 atcctccaca gcatcctgaa gggtacctgg caggtggcgc tcgtcaccct gcacctccgc
    23521 ccgctgagca gcccgggtat cgtggcggtt ccggtggggg agaggacgcc cgcgggcgtc
    23581 gccttctccg cgctggcgac caccctcgcc cccggaacct tcctcgtgga tgtcgactgg
    23641 gagagagggg tgatgctcat ccacaccata gacgcctccg atccggaggc cgtacgccgg
    23701 gagcacgagg agttctaccg gcgctaccag agagaggtgt tcccctgaga gcaaaggagg
    23761 ttcccgcggg atgcacgaag cggtcttcta cgcggcgtcg gtctggatgg cgctgctcct
    23821 gctcaccagc gtgtacgcgg tgatccggat gccctccacc accgggcgga tactcgccct
    23881 ggacaccctc acgctggtga tggtgggcat cctggtgctg ttcgtggacg ccttccgcac
    23941 cccctacttc ctcgacgcgg cgctcatcct cgcgctgctg gcctttgtgg gcaccgtggc
    24001 cgccgcccgc taccgctccg agaggaggct cttctagtgg gcgccctcct tccgtggctc
    24061 gccgacgccc tggtgatcct cggggtcgcg gtcataaccg tcggggtgta cggtatgatc
    24121 cggatgccgg acacctacac ccggctgcac gcggcgagca aggccgtctt tctcggtgtc
    24181 atctcgcttc tggccgcctc agtggtgacc gggcagcccg cgatcatcct ccgggtgatc
    24241 ctgatcgccc tcttcctcct gatgacctcg ccggtgtcgg cgcacgtgat cgggcaggcg
    24301 gcttaccgca ggggggagga gatgctgtcc ccggacgcgg tggacgagtc gaacaggtac
    24361 ccaccgggga cgtagtaggc tctctctgta tgctcaacag gtttccgcag agggtgctgc
    24421 tggcgaccga cggggctgcg ggagcggcgc tggccctgca cgcggcagcc gagatctgca
    24481 cgagggccga ctcggaattg cacctcgtgc acgtctggcg taccttggaa tccggccccg
    24541 acacggagct gcgcaggcag gaggcttgct ggcttttacg cagggagatc ctggcggcgg
    24601 aggcagcctt ggggggagag gtaaaaggat acctcaggga ggggatgccg gcggaggaga
    24661 tctccgcgct ggcaagagag ttggaggccg gcttgatcgt catgggcagc cgggatctag
    24721 gccctatgga gcggctggtg atgggcagcg tctcggaggg agtggtgaac caggctccct
    24781 gtcccgtgct ggtggtgagg ggcggggcgt ggccaccctc gcgggtggtc gtcggcgacg
    24841 actcctctga gggcgcgcgc cgggccggag agcttgccgc gcgcatcgcc gtcctccttg
    24901 gtgcaacgct gcaactggtg agggcttacc ccgtggtcct ggacatctcc gaggccgcaa
    24961 ggttgtcgga gagcattgcc gtgcccctgc gggtggcgat gagccggcac gaggctgcgc
    25021 tgctgggacg agcccgggct ctggaggggg ttcttgggta tcgtccgcgg ataagggtga
    25081 tggagggaga agcagcatcc gtgattctcg ccaccgccga agagggtgac ggggggccgg
    25141 ccctcatcgc agttggcagg cgcgggctcg gggagctgga ccggctgagg ctcggcagcg
    25201 tctccaccaa gatcttgcgg ttgtccaggg ggccggtgct gatctgtccc gcatgagaca
    25261 cgcgagccct gctcagtctt cttgtctctc gtggtgaacg atgcctttga cctcctcgac
    25321 ggccatcacg aacgctatgc cgcgggctgg ctcgtcgagc cgcccggccc gcaccaccgc
    25381 gtcgaggatc gcatcccgct tctccggctc cacgaggatc agaaccagct ctcgctcgga
    25441 gctgaccgtg atgccgaaga cgcgtctggc ctcgtgcacc ccggtgccgc gggccattag
    25501 gatggtcccg ccctcggccc cagcttcccg ggcagcgtcc atgaccttcc cggctgctcc
    25561 tttcggaact atggcggcga ttagcttgta tccccgcacc tcgtt
//
