LOCUS       NZ_AP019791            10297 bp    DNA     circular CON 25-APR-2024
DEFINITION  Rubrobacter xylanophilus strain AA3-22 chromosome, complete genome.
ACCESSION   NZ_AP019791
VERSION     NZ_AP019791.1
KEYWORDS    RefSeq.
SOURCE      Rubrobacter xylanophilus
  ORGANISM  Rubrobacter xylanophilus
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae; Rubrobacter.
REFERENCE   1
  AUTHORS   Tomariguchi,N. and Miyazaki,K.
  TITLE     Complete Genome Sequence of Rubrobacter xylanophilus Strain AA3-22,
            Isolated from Arima Onsen in Japan
  JOURNAL   Microbiol Resour Announc 8 (34), e00818-19 (2019)
   PUBMED   31439702
  REMARK    DOI:10.1128/MRA.00818-19 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022875)
  AUTHORS   Miyazaki,K. and Tomariguchi,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2019) Contact:Kentaro Miyazaki National Institute
            of Advanced Industrial Science and Technology; 1-1-1 Higashi,
            Tsukuba 305-8566, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Coverage                   :: 758x
            Sequencing Technology             :: NovaSeq6000; GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007164525.1-RS_2024_04_25
            Annotation Date                   :: 04/25/2024 02:58:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,085
            CDSs (total)                      :: 3,032
            Genes (coding)                    :: 3,015
            CDSs (with protein)               :: 3,015
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 9 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:10:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2236164
            Orig. end                         :: 2246461
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP019791.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Annotated at DFAST https://dfast.nig.ac.jp/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(531..1433)
                     /locus_tag="RxyAA322_RS11420"
                     /old_locus_tag="RxyAA322_22810"
     CDS             complement(531..1433)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="RxyAA322_RS11420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_22810"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_143528431.1"
                     /transl_table=11
                     /translation="MHADAIVETRNLTKRYGRINAVDGLDLTVRRGEVYGFLGPNGAGK
                     TTTLRMLLGLVRPTSGSASVLGGEPGAPEGLKRVGALVESPAFYPYLSGRDNLRVIARY
                     CGAPPSRVDEMLETVELSGRAKDRFKKYSLGMKQRLGVAAALLKDPELLILDEPTNGLD
                     PKGMADMRDLIRRLGRGERTVLLSSHLLGEVEQICDRVGVIRKGHLVAEGTVAELRSKA
                     GLLVRAAPLEDAAKIAEGLVGVEEVEVSGGLLRLTTDPGRAAEINRRLVSAGLDVSELR
                     PAEQSLEEVFLELTAEEVV"
     gene            complement(1440..2264)
                     /locus_tag="RxyAA322_RS11425"
                     /old_locus_tag="RxyAA322_22820"
     CDS             complement(1440..2264)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /locus_tag="RxyAA322_RS11425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_22820"
                     /product="stage II sporulation protein M"
                     /protein_id="WP_274596107.1"
                     /transl_table=11
                     /translation="MFYPGACLAGRFLRRNAILFALCLSCYLAGALAGGVMAVNGFSGG
                     ETIGADQFGAPGDPLGIASNNLVAVALVALGPVTFGVLAALLLFGNGMVAGIAVGAKLN
                     EGWSPVEITAGVLPHGLLEIPGLLFAGVVGFKGLHWCLATIKGKSVENPFGDCLVGLAL
                     AALLIICAAPVEAYGRPCLWKRSEEENLMREGRDRDLDTSEKLLVLAIPASAISALVVS
                     FFEPRLVLLFLFGGPCAAFVARPLRDSKTLRFIAAGGFALLLISTVVMFLEG"
     misc_feature    complement(1473..1475)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(1719..1721)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2315..3037)
                     /locus_tag="RxyAA322_RS11430"
                     /old_locus_tag="RxyAA322_22830"
     CDS             complement(2315..3037)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="RxyAA322_RS11430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_22830"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_143528433.1"
                     /transl_table=11
                     /translation="MFPIELDGVTKSYGEVRALDGVSLRVRAGEIHGLLGPNGAGKTTL
                     MRIIAGLVPQDSGEVFIAGLNVRDAPRRTRRAVGFVPDSPFLYRYLTGRQYLEFAADLW
                     GLPEDEKIKAVEDGLKRFGLADRAQHVIGSYSFGMRQKLAVAGALLQGPTVLVLDEPLT
                     GFDPPSSRFMKDFLRSFADAGRAVFLSTHILELAHGLCDRVSIISDGRIILERETDGDA
                     TGFERSVLNAMDRSGGSS"
     gene            complement(3041..4903)
                     /locus_tag="RxyAA322_RS11435"
                     /old_locus_tag="RxyAA322_22840"
     CDS             complement(3041..4903)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RxyAA322_RS11435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="RxyAA322_22840"
                     /product="hypothetical protein"
                     /protein_id="WP_172620815.1"
                     /transl_table=11
                     /translation="MSPKPRIELRSIERVLRKGSPAERIAGRGPAGGAHRFGGDGAWRA
                     ALALSLVDFHRAYNRLLEALPGTLESSLRERSFRTRPLGWFIVGLAVAALIATVAFALF
                     DAVFEAIEIAVGAGMADLKEIGAIAVVTVLLGETRGAVKGLERAAHPEDYELLYRSPVP
                     ASAFVLCRFLLAAAVRSTSIWLFVVGPAVAASIWILGNSPLAFLAWAALYAPFLAAVVT
                     LRSAWGVVGYSIKSSTHAAFGSSAAGIALGCGVAAGLAGVLALLVTRLLGGAQEVVASI
                     RTFLASRSVETFAERAWLPSNWFVFSLADAASGRAVSAFWWTVLAYVVVVPVAIIVMWA
                     TARLIPMGSQWGYERSMREGGVTRFVASGVSAVAGRFGPILAKDLRALSRATPVVRRRF
                     STMLFLMATLIGFVVGLGVGGAYQAHSWQAVFPLLIFSYTLCSFAGEALLPVTAIDAEG
                     EAIDLLRRASVPLMRIFAAKATLQVVLLTVFCSTLLVSSHAFLRYPSALFGAALCIGIA
                     SALACGVSQVGSSAVYPRFDWEHHREIGNSPRASTLSSIASGLYLTLALEAAAICGILH
                     YFGYLEEDTMLLATGAGVLLSAALVTFGLALLVRQRGHRHWGGA"
     misc_feature    complement(3677..3679)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4109..4111)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4972..4987)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.74"
                     /tool="antismash"
     gene            complement(5001..5297)
                     /locus_tag="RxyAA322_RS11440"
                     /old_locus_tag="RxyAA322_22850"
     CDS             complement(5001..5297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03651.1"
                     /locus_tag="RxyAA322_RS11440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_22850"
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="WP_197735471.1"
                     /sec_met_domain="TIGR03651 (E-value: 1.4e-10, bitscore:
                     38.8, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALVVAMAITYFMVASGSLPSIGNTNGLSAGIADLATNLGISYYA
                     AKKAIDIIVTFSNIALILSLLASVVGAGIFTAGIVAIAKRLALRYGERYAAAW"
     gene            complement(5473..6153)
                     /locus_tag="RxyAA322_RS11445"
                     /old_locus_tag="RxyAA322_22860"
     CDS             complement(5473..6153)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /locus_tag="RxyAA322_RS11445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_22860"
                     /product="YIP1 family protein"
                     /protein_id="WP_143528435.1"
                     /transl_table=11
                     /translation="MGGTAGHVVRRKTHRNLGLIDSALDTIINPRRAFSRVAEHPRILP
                     PLLFVVIATLVMYIALGSAFETRLLEALERAGIEATPRLVWLVLGLGGVFGLVQVVVFT
                     LVAGLITHAVARLLGGSADAHCSVACYALAMIPSGFKAITVAVLAFLLGAENVPLTTGG
                     VYSGGALLTLFDPFSLWAALLLGMGLAKIHGLSIVRAFGISFSIFVVGWAMSAALTAAQ
                     GALV"
     misc_feature    complement(5599..5601)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(5881..5883)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6753..7658)
                     /locus_tag="RxyAA322_RS11450"
                     /old_locus_tag="RxyAA322_22870"
     CDS             complement(6753..7658)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (25-161). E-value: 1e-12.
                     Score: 40.1. Matches aSDomain:
                     nrpspksdomains_RxyAA322_RS11450_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /locus_tag="RxyAA322_RS11450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_22870"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_143528436.1"
                     /sec_met_domain="PF00561 (E-value: 1.5e-09, bitscore: 35.4,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.4e-12, bitscore:
                     45.4, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSARKSGFAEVDGAKIYYEVEGSGDPVVLIHQRGVDLRVWDDQSP
                     AFSKRYTVVRYDRRHWGKSQVESWGYPAAPEDCDLESLGQWLEDQPLSPAPYEDLRELL
                     DYLGIVRAHLLGLHDGGEVALEFALEYPQRVGGLVLVDTVVPSAGSAQQFEDLAERGLH
                     KAAELQEDVLEEALGKVDVTEIVEWVLDDPSYAASTEATRQRLRRIYADNASANLMPGD
                     HNVRRMSPPAGERLEDVKAPTLLILTGERPPYVSELDRALAEGIGAPTTVVTMPDASPT
                     VNMDRPEEFNRTVINFLDDL"
     aSDomain        complement(7176..7583)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_RxyAA322_RS11450_Abhydrolase_1.1
                     "
                     /evalue="1.00E-12"
                     /label="RxyAA322_RS11450_Abhydrolase_1.1"
                     /locus_tag="RxyAA322_RS11450"
                     /protein_end="161"
                     /protein_start="25"
                     /score="40.1"
                     /tool="antismash"
                     /translation="PVVLIHQRGVDLRVWDDQSPAFSKRYTVVRYDRRHWGKSQVESWG
                     YPAAPEDCDLESLGQWLEDQPLSPAPYEDLRELLDYLGIVRAHLLGLHDGGEVALEFAL
                     EYPQRVGGLVLVDTVVPSAGSAQQFEDLAERG"
     gene            complement(7825..8808)
                     /locus_tag="RxyAA322_RS11455"
                     /old_locus_tag="RxyAA322_22880"
     CDS             complement(7825..8808)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="RxyAA322_RS11455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="RxyAA322_22880"
                     /product="hypothetical protein"
                     /protein_id="WP_143528437.1"
                     /transl_table=11
                     /translation="MQRIEEAVGDKLEVRSLHEPQVEHWREQALGSDAPWAPTLIEVEG
                     ERVRAWVGVRMGAVLARRLGPAATWRVMQALGEAGTPSTGVASMEESGGISRGQFLKGL
                     TGGLVALSVLPAGQALASGKEAVNASGVTRVSSRSAAVARLKRSRAVSAAAGRFGSPDW
                     SGVTRAKYKDKDGVERAFYTVPYRLAEATAAGATAGVTLLFTEDEGSVEDAQSVALRVR
                     QTGEGRVTLEYYLPDGTPVATVEEQDGKIKARPAERSSQDAVQPRGVREFVGCFVGCLG
                     ASVSVGCAVACANCVIPPPEPPPCIQCAACAGSAGLRCARLCRPLL"
     misc_feature    complement(8203..8205)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9100..9945
                     /locus_tag="RxyAA322_RS11460"
                     /old_locus_tag="RxyAA322_22890"
     CDS             9100..9945
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /locus_tag="RxyAA322_RS11460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="RxyAA322_22890"
                     /product="transposase"
                     /protein_id="WP_172620816.1"
                     /transl_table=11
                     /translation="MTVLFCLIDDAYALLNPDGRSHESLKRLSDSEVLTLALFQQLRGV
                     ESERSFLRDAQRFFSHLFPGVAGMHPSSLHRRLRKLRRFLEPLRRFVLPELVGDPETLV
                     VDSTLLGVLHPRQVKQSAAGFEGAAWVKWGSFAVYGVKLHLICSTNRIPISYELTGANI
                     ADVLLVRELLVGAGLDEASVARRLLGDLAYCSGALGEELAKRGILLATEKADRRPPIRQ
                     QVEVCFAALKRVFGMDGTLAKTLTGLATRIAAKVAAYTYGCYVNRILGRPQGRIKELWA
                     "
     misc_feature    complement(10272..10287)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.25"
                     /tool="antismash"
ORIGIN
        1 ggcgaggatc agccagcccg ccccgagcgc ttcgagccac cggcctgcag atggccagct
       61 tacgggggct ccttcggcga gggagaccga gtagctgcac agggcgccga gcaccaggcc
      121 ggcggcagcg ctgacgagca ctacgacccc gaccgcggtt actttgcccg cgagcacaga
      181 gagcctaccc ggacgctgcg tcaggacgat cttcagggta tcccatccgt attcgccgct
      241 gacagacagc gcgccgaaga taagggccac ccctgcgccg atccctccga acgaccgcag
      301 ggtggtggcc agcacgtttc gcgggtagag ctcggcgaca gcgccctttg ccgcgaccgg
      361 tgcatcctgc cggggtccct gcgaggaagc cccgccgtcc agcgccgtga gcagcagata
      421 tgtaagcaga taccccaaaa gcacgatcga gagcccgaag atcgtccaga ggatccacgt
      481 cgccgggcgc ttcctgagct tgagcagctc cgccgcgaag ctcgcgagca tcagaccact
      541 tcctctgcag taagttccag gaagacctcc tccagcgact gctccgccgg cctgagctcg
      601 ctgacgtcca ggcccgctga gacgagcctg cggtttatct cggcggcgcg tcccgggtcg
      661 gtggtgagcc tcagcaggcc gcccgagacc tcgacttctt cgacgcccac gagcccctcc
      721 gctatcttcg cggcgtcctc cagcggcgcg gcgcgcacga gcaggcctgc tttactgcgc
      781 agctcggcga cggtgccctc ggcgacgagg tggcccttgc ggatcacgcc gacccggtcg
      841 cagatctgct cgacctcccc gaggaggtgg ctggagagca gcacggtcct ctcgccgcga
      901 ccgagcctcc ttatcagatc gcgcatgtcc gccatcccct tcgggtccag cccgttggta
      961 ggctcgtcga ggatgaggag ctccgggtcc ttcaggagcg ccgcggccac cccgagccgc
     1021 tgcttcatcc cgagcgaata tttcttgaac ctgtctttgg ccctccccga gagctcgacc
     1081 gtctcaagca tctcgtccac ccgtgagggc ggcgcaccac agtagcgggc gatcaccctg
     1141 aggttgtccc tgcctgaaag gtaggggtag aaggcggggg actccaccag cgcgcccacc
     1201 ctcttcaggc cctcaggagc ccccggctcc ccgccgagca cgctcgccga accggaagtc
     1261 gggcggacca ggcccaacag catcctgagg gtggtggtct tgccggcgcc gttggggccg
     1321 aggaagccgt agacctcgcc ccggcgcacc gtgaggtcga ggccgtccac ggcatttatc
     1381 cggccgtagc gcttggtcag atttcgggtc tcgactatcg catcagcgtg catgcaggat
     1441 catccttcca agaacatcac cacagtcgat attaagagga gagcgaagcc gccggcggct
     1501 atgaacctca acgtcttcga gtcccgaagt ggtctggcga caaaggccgc gcaaggtccg
     1561 ccaaagagaa acagcagcac gagcctgggc tcgaagaacg agacgacgag agcggagatc
     1621 gcgctggcgg gtatggccag taccagcagc ttctccgatg tgtccagatc tctgtctctc
     1681 ccctctcgca tcaagttctc ctcctcagaa cgcttccata agcacgggcg tccgtacgcc
     1741 tcaacgggcg cagcgcagat tatcagcaga gccgcgagcg ccaggccgac caggcagtct
     1801 ccgaacgggt tctccactga cttgcctttt atcgtcgcca gacaccagtg caaacccttg
     1861 aaaccaacga caccagcgaa cagcagcccg ggtatctcga ggagaccgtg aggcaggacc
     1921 cccgcggtga tctccaccgg agaccagccc tcgttcagct ttgcgccgac tgctataccg
     1981 gcaaccatcc cgttgccgaa caggagaagc gccgcgagga cgccgaaggt gacagggccg
     2041 agcgccacca gcgcgaccgc tacaaggtta ttgctggcga tcccgagcgg gtcgcctggt
     2101 gccccgaact ggtctgcgcc gatcgtctca cctccagaga agccgttcac agccattacg
     2161 cctcccgcaa gggcacccgc cagatagcaa ctcagacaga gagcgaagag gatcgcatta
     2221 cgccgcagga aacgtcctgc caggcaggcg ccggggtaga acactgactc caacggcccg
     2281 ggggtggtcc tgctctgtga cgggtcgcgc tcgctcaaga tgagccaccg gatcgatcca
     2341 tcgcgttcag caccgaccgc tcgaaaccgg tcgcgtctcc gtcggtctca cgctcaagga
     2401 ttatcctccc gtcggatatt atgctcacgc ggtcgcagag gccgtgggca agctctagga
     2461 tgtgggtcga caggaaaaca gctctcccag cgtctgcgaa ggagcgcaga aagtctttca
     2521 tgaaccggga gcttggcggg tcgaacccgg tcagaggttc gtccagaacg agcacggtcg
     2581 gcccctgcag aagtgctccg gccacggcca gcttctggcg cataccgaac gagtacgagc
     2641 cgatcacgtg ttgcgcgcga tctgcgaggc caaaccgctt caaaccatcc tcgacggcct
     2701 ttatcttctc atcctccgga agaccccaga gatcggcggc gaactccaga tactgccgcc
     2761 cggtcaggta cctgtagagg aagggcgaat ccggtacgaa gcccacggcc ctgcgtgttc
     2821 gccgtggagc gtctcgcacg ttcagacccg cgatgaacac ctctccggag tcctgcggga
     2881 ccagcccggc gatgatgcgc atgagggtgg tcttgccggc accgttgggg cccagcaggc
     2941 cgtggatctc ccctgcccgc acccggagcg acactccatc caatgcccgc acctcgccgt
     3001 aagacttggt gaccccgtct agctctatcg gaaacacgga tcaggctcct ccccaatgcc
     3061 ggtgcccgcg ctgccggacc aacagtgcca atccaaaagt aaccaacgcg gccgacagga
     3121 gcacacctgc tccggtggca aggagcatcg tgtcctcttc caggtaaccg aagtagtgca
     3181 ggatgccgca tatcgccgcg gcctccagcg ccagcgtcag gtagaggccc gaagctatgc
     3241 tggagagcgt cgaggcgcgt ggagagttgc cgatctcccg gtggtgctcc cagtcgaacc
     3301 tgggatagac cgcgctgctc cccacctgac tcaccccaca cgccagcgcg gacgcgattc
     3361 cgatgcacag cgcggcaccg aacaacgctg acggataccg caagaaggcg tgagaggaaa
     3421 ccaacaaggt tgagcagaac accgtaagga gcacgacctg aagggtcgcc ttagccgcga
     3481 agatgcgcat cagcggcacc gacgcccgac gcaacagatc tatggcttca ccctccgcgt
     3541 cgatcgccgt cacgggaagc agagcctccc cggcgaaaga acacagcgta taagagaaga
     3601 tcagaagagg aaaaacagcc tgccacgagt gagcctggta ggcgcctccg acaccgagcc
     3661 cgaccacgaa gccgattaag gtagccatca ggaacagcat cgtcgaaaac cgtcgacgca
     3721 ccaccggagt tgcccgggat agagcgcgaa ggtctttggc aaggatggga ccaaacctgc
     3781 cagccacggc ggacacgcca gaggcgacaa accgggtaac cccgccctcc cgcatggatc
     3841 gctcgtagcc ccactgggag cccatcggta tgaggcgcgc agtcgcccac atgacaatga
     3901 tggcgacggg aacgacaacc acgtacgcga ggacagtcca ccagaaagcc gacacggccc
     3961 gtccggaagc cgcgtccgcc agcgagaaga cgaaccagtt agaaggaagc caggcccgtt
     4021 cggcgaaggt ttccacgctg cggctcgcta gaaaggttct gatcgaagcc acaacctcct
     4081 gcgcccctcc caacagacgc gtaacgagta acgcaaggac gcctgcaagc cccgcggcga
     4141 ccccgcaacc cagcgcgatg ccggctgcag aggacccgaa agccgcgtgc gtggagcttt
     4201 tgatgctgta cccgaccacc ccccatgcgc tccgaagggt gaccacagcc gccaagaagg
     4261 gtgcgtacag ggccgcccag gccagaaatg cgagcgggga gtttccgagg atccagatag
     4321 aagcggctac agcggggccg accacgaaga gccatatcga ggtcgaacgc acggcagcgg
     4381 cgagcaagaa gcggcacagc acgaaggcgc tcgctggtac agggctccgg tagagaagct
     4441 cgtagtcctc cggatgagcc gcccgctcca gacccttgac agcacctctg gtctctccca
     4501 gcaacaccgt gaccacggcg atggcaccta tctccttcag gtccgccatt cccgccccca
     4561 ccgcgatttc gatggcctcg aaaacggcgt cgaagagcgc gaacgcaacc gtcgcgatga
     4621 gcgcggccac ggccagcccg acgatgaacc acccgagagg tcgagtccgg aacgaacgtt
     4681 ccctgagcga cgattccagg gtcccgggaa gggcttcgag cagtcgattg tacgctctat
     4741 ggaaatccac gagcgacagc gcgagcgccg ccctccaggc accgtccccg ccgaaacggt
     4801 gagcaccacc agccgggcct cgtcccgcga ttcgctcagc gggcgaaccc ttccggagaa
     4861 ctcgctcaat actccgaagc tcgatccttg gtttgggact cacgggccaa tctctcttct
     4921 ctgcgctgca gatcggggct acgacctagc cggccgagcc tcgatctgcc gtgtacgcaa
     4981 tcggcgaccc cggatacccc ctaccaggct gccgcgtacc tctcgccgta acgcagcgcc
     5041 agcctcttgg caatggcgac gatgccggca gtaaagatgc ccgctccgac aaccgaagcc
     5101 aaaagcgaga gtatcaacgc tatattggag aaggtcacga ttatatcgat ggcctttttg
     5161 gccgcgtagt agctgatgcc gaggttggtc gcaagatcgg ctatgccggc ggagagaccg
     5221 ttcgtatttc ctatagaggg caggcttccc gacgcgacca tgaagtaggt gatcgccatc
     5281 gccacgacca aggccaacgc agcgaccaac ctaaacgcta agcctctgct taactgcaca
     5341 aactgcacgt aatctccctt cttcagtcat gtccgctcac atcttcggga tcacgcaacc
     5401 gcgcctgatc cacgttcacc aactcacctc atgcgaccac ctccttcctc aactcctccc
     5461 atgagactcc ttctatacaa gcgccccttg tgcagcagtt agggcagcac tcattgccca
     5521 gccgactacg aatattgaga acgagatacc aaaggctctg acaattgaaa gaccgtgaat
     5581 cttggcgagt cccatcccta agagcagggc ggcccataga gaaaaaggat caaatagggt
     5641 gagcagcgct ccaccggagt agacgccacc cgtggtgagt ggaacattct ctgcacccaa
     5701 gagaaatgcc aatacagcca ctgtaattgc tttgaaaccg ctaggtatca ttgcaagggc
     5761 atagcatgcc accgagcagt gcgcatcggc cgatccaccc aacaggcgag ctaccgcatg
     5821 ggtgatcagc ccggcgacga gcgtaaagac cacaacctgc accaacccga agacgccacc
     5881 taagcccagg accagccaca ccaaccgagg tgtggcctct atcccggcac gctcgagtgc
     5941 ctctagaagg cgggtctcga acgccgaacc gagggcgatg tacatgacga gagtcgcaat
     6001 caccacgaac agcagaggag gaagtatccg agggtgctca gccacccgcg aaaacgctcg
     6061 acgcgggttg attatcgtgt cgagcgcact atcgatcagg ccgagatttc gatgcgtttt
     6121 gcgtcgtacc acgtgccctg cagttccacc cacggattat ctacctcact gacttcggct
     6181 tgcacggaca ctctagagga ggttgtggat ctcaaaacct ctctccgacc gcctcctcga
     6241 tcctccgtgc cagagcaccg tacgccgcac atccggcgtc gtagcccaga acgagccgcc
     6301 tgcccctcat gctctctccc cctttgctct ctgctccctg caggaaacac cttagagcaa
     6361 gagcttcacg ggcacatcat cctcgaggta tgaatttcgg tatgaatttt tctcatacca
     6421 ttctctagag tgcatcagga gctcatgtgg tcgatgtaac ggtccgtgtc gcccgtgtca
     6481 cgcagataca gccccacgta atagcggaca aaagcgctca tctctgacct cgctttggcc
     6541 cgcggagaag cacgtagatt ccggcgcggt aagctctcga tgacgccaga gcctcagcat
     6601 ctactcacta ctctgcataa acggctttct agcgctccac gttttgccag ccaagtcaga
     6661 taccgtactt cagacgatac tgcgtgaggt aggctagagc gaaggtgggt cgtcggagca
     6721 acggactccc ggctgcgcaa aatcgaggcc cgctacagat cgtccaagaa gttaatgacg
     6781 gtacggttga actcttccgg acggtccata tttacggtcg gggaagcgtc tggcatcgtc
     6841 accaccgttg tcggagctcc tatgccctct gccagggctc ggtcaagttc gcttacgtag
     6901 ggcggacgct ccccggtcaa gatgaggagg gtcggtgctt tgacgtcttc gagcctctct
     6961 ccggctgggg gagacatcct ccggacgttg tgatccccgg gcatgaggtt ggcagaggcg
     7021 ttgtcggcat agattcgtcg caacctctgg cgcgtggcct cagtggaggc cgcgtacgag
     7081 ggatcatcga gcacccactc gacgatctcc gtaacatcga cctttccaag agcctcttcc
     7141 agcacgtcct cttgcagctc cgctgccttg tgcaggcccc gctcggccag gtcctcaaac
     7201 tgctgcgcgc tccccgccga ggggacgacc gtgtccacca agacgagacc gcccaccctc
     7261 tgtgggtatt ccagagcgaa ctccagggcc acctccccgc cgtcgtgcag gccgagcaga
     7321 tgggctcgca cgataccgag atagtcgagc agttcccgca ggtcctcgta aggggcagga
     7381 gaaagaggct gatcttcgag ccactgaccc agactctcga ggtcacagtc ttccggagca
     7441 gcagggtagc cccagctttc gacttgagat ttcccccaat gccggcggtc gtagcgaacg
     7501 accgtatagc gcttggaaaa cgccggactt tggtcgtccc acaccctcag atcaacacct
     7561 cgctgatgga tcagcacgac ggggtctccc gatccttcca cttcatagta gattttcgct
     7621 ccgtccacct ccgcgaaacc cgactttctt gcgctcatca aacctcccgg acgctcagac
     7681 tagtgtcgcg cagcttagtg tcgcgcagct ttggcgagga gaaggagacc gagaccccat
     7741 cttggcgcac actctgactc gtgaacctcc ggatggagcc cggctcctcc ccaatctcac
     7801 ctcgcacgcc tccgggacct tagcttacag gagtggcctg cacagccgag cgcacctcaa
     7861 tcccgccgaa ccagcgcaag ccgcgcattg gatgcaaggc ggaggttcgg gaggaggtat
     7921 gacgcagttt gcacaagcta cggcgcagcc gacactgacg ctggccccga ggcagcccac
     7981 gaagcagccg acgaactccc ttacgccccg cggctgcacg gcgtcttgcg acgaccgttc
     8041 cgccggtcgc gccttgatct tgccgtcctg ctcctcgacc gtggctacgg gggtcccgtc
     8101 cggcagataa tattcaagcg tgacccgccc ctcaccggtc tgacgcactc gcagggcgac
     8161 gctctgtgca tcttctacgc taccctcatc ttccgtgaag agtaacgtga cccccgcagt
     8221 ggctcccgcg gccgtcgcct ccgccagccg gtaggggacc gtgtagaacg ctctttctac
     8281 tccgtccttg tctttgtatt tggcccttgt gacgccgctc cagtctgggc taccgaatct
     8341 accggcagca gcgctcacgg ctcgggaacg ctttagccgg gcgacggcag ctgatcggga
     8401 cgaaaccctg gtgactccgg aagcgttgac ggcttccttg ccagaggcca gagcttgccc
     8461 agccggcaac acggagaggg cgaccaatcc tccggtcaaa cctttgagaa actgcccgcg
     8521 gctgatccca ccggactcct ccatggaagc caccccggtc gagggcgttc cggcctcccc
     8581 cagcgcctgc atgacacgcc aagtggcagc cggcccgagc cgccgggcaa ggaccgctcc
     8641 catcctcaca ccaacccacg cccgcactct ctcaccttcc acttcgatca gcgtcggagc
     8701 ccacggcgca tcgctaccca gcgcctgctc gcgccagtgc tcgacctgcg gctcgtggag
     8761 gctgcggact tccagcttgt cgccgaccgc ctcctctatg cgctgcacca gagcgctgca
     8821 tgccccacac cctccgtcgt agcccagaac gagacgcctg cctctcatgc tctctccccc
     8881 tttgctctct gctccctgca ggaaacacct tagagcaaga gcttcatggg cacatcatcc
     8941 tcgaggtatg aatttcggta tgaatttttc tcataccata ctctagatgc gtgttgcgtg
     9001 gtttgacggt aaaacggggg ccatccgaat ccccagcgaa aggcacctcg gatggcccac
     9061 acccagcata cccccactct cgcctccctt gaagaggccg tgaccgtgct tttctgcctc
     9121 atagacgacg cctacgcgtt gctcaaccct gacgggcgaa gccacgagtc gctcaagagg
     9181 ctctcggact ctgaggtctt gacgttggcg ctcttccagc aattgcgggg tgtggagagc
     9241 gagcgctcct tcctgcgcga cgcccagcgc ttcttctccc acctcttccc gggggtggcg
     9301 gggatgcacc cctcctcgct gcaccgtcgc ttgaggaagc tgaggcgttt cctggagccc
     9361 ctgcgccgct ttgtcctgcc cgagttggtt ggtgatcccg agacccttgt cgtcgactcg
     9421 acgctgcttg gggtcttgca cccgcgccaa gtgaagcagt cggcagcggg ttttgagggg
     9481 gcggcatggg taaagtgggg atcgttcgcc gtctacgggg tgaagctgca cctcatctgc
     9541 tccaccaacc gcattcccat ctcctacgaa ctcaccggcg ccaacatcgc ggacgtgctg
     9601 ctggtgcggg agcttttggt cggagccggt ctcgatgagg ccagcgtcgc gaggagactc
     9661 ttgggggacc tggcttactg cagcggggcg ctcggggagg aactggcaaa gcgcggcatc
     9721 cttctggcca ccgagaaagc ggacaggcgc ccgccgatcc gccagcaggt ggaggtgtgc
     9781 ttcgcggccc tcaagcgcgt tttcgggatg gatggcacgc tggccaagac gctcaccggg
     9841 ttggcgacca ggattgcggc gaaggtggca gcctatacct acggctgcta cgtcaaccgc
     9901 atcttgggca ggccgcaagg gcgcatcaag gagctgtggg cctgatgtcc tcgcaacact
     9961 catctagagc tgctcttctc cgtctcccgg aaggagatcc cttctgcctg agactccgag
    10021 tttcttgagg atgttggtga cgtggctctt tacggtaggg agcttggcac cgaacatgac
    10081 tcaaacatga ctgtatgacg aactaccgtt aaactgaaga tgttcaatcg gatcgtgcgg
    10141 actccgagac ggaacggacc tcagttgcgt ccatcttcgt catcgactgt ttgcagagat
    10201 ggtccctggc tccacccaga agactcgagg tacaaccggt tatcagatct atctgtggta
    10261 cgatcgccag agtattcatt tgtaaatgag aggcccc
//
