LOCUS       NZ_CP041604            10798 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. S1A1-7 chromosome.
ACCESSION   NZ_CP041604
VERSION     NZ_CP041604.2
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. S1A1-7
  ORGANISM  Streptomyces sp. S1A1-7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11713216)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Massive gene flux drive genome diversity within a population of
            Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11713216)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 11713216)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2019) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007113565.2-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:07:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,902
            CDSs (total)                      :: 10,801
            Genes (coding)                    :: 10,337
            CDSs (with protein)               :: 10,337
            Genes (RNA)                       :: 101
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 464
            CDSs (without protein)            :: 464
            Pseudo Genes (ambiguous residues) :: 0 of 464
            Pseudo Genes (frameshifted)       :: 185 of 464
            Pseudo Genes (incomplete)         :: 374 of 464
            Pseudo Genes (internal stop)      :: 46 of 464
            Pseudo Genes (multiple problems)  :: 127 of 464
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:07:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 11181180
            Orig. end                         :: 11191978
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041604.2.
            On Mar 4, 2020 this sequence version replaced NZ_CP041604.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="42"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(766..782)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.97"
                     /tool="antismash"
     gene            complement(862..2586)
                     /locus_tag="FNV64_RS51260"
                     /old_locus_tag="FNV64_51920"
     CDS             complement(862..2586)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018468799.1"
                     /locus_tag="FNV64_RS51260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_51920"
                     /product="FUSC family protein"
                     /protein_id="WP_143656725.1"
                     /transl_table=11
                     /translation="MSRHHRTTLTRVLSPRGALTLHAVDGALLFALRAALAMALPAAPL
                     VLSGRTALAVFPMLGAFTTTFGRNLPYRRRARVLAVAAVAMTMCVGLGATLAALIDPRA
                     GGLGAAVVIAAMAVTAGLAKFLCDATRLSGLGAVLMLFSFAVAANGPADGLTDVLAQTG
                     LAAAGAATAWALALVGWFVHPDRPQRLAVAVALRELADLLEPGGGADGTGHTRHRATAA
                     VLRAYQSLGLSPLTTDQRHGERSGTCVFLTDLAWSLLITSARRQTPDAPTARYLRVQAR
                     YLTVRHRRPPAPLPHPPFPSAPSAPSADTTPGRPDDAGPAARRAAELTVGTGAGSPGHV
                     AVLVVPAFRMALGTGLAGGLAAFLSFGHGYWAALSAAAVLHSVNVRSTLERALQRTLGT
                     AAGLLIAFGVLATHPAPTALVALIVVFEFLLEYTVARNYGLGVVFLTPLALLMSDLASP
                     APVGTLVHDRTLGSVLGILIGLACALLVVHGRPAARVERALAACCEASERAEQALADSA
                     AVPHPAVQTQLAVAVVELREADDAAAGELYAADVDPAQLAAAEQRAYVLLERLHRTT"
     gene            complement(2687..3313)
                     /locus_tag="FNV64_RS51265"
                     /old_locus_tag="FNV64_51925"
     CDS             complement(2687..3313)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952221.1"
                     /locus_tag="FNV64_RS51265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_51925"
                     /product="DUF1345 domain-containing protein"
                     /protein_id="WP_054228053.1"
                     /transl_table=11
                     /translation="MSIRLSLSAVPRLAISVAVGAAVGVAVALSSDTPLAILAGIAATQ
                     TFFVVVGWIVLWPMDAAATRRNARREDLRPVLEEIVVVTVALCGLVGIVVFLLGNSDSG
                     HAATALLGVFMAWAALHLMYATRYAYLYYADSAGGIDFNSDQPPAYRDFFYFSYNLGMT
                     YQVSDTDVSSAVIRSMVLRHTLLSYVFGTSILATAINLFAGILVH"
     gene            complement(3459..3932)
                     /locus_tag="FNV64_RS51270"
                     /old_locus_tag="FNV64_51930"
     CDS             complement(3459..3932)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024426.4"
                     /locus_tag="FNV64_RS51270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_51930"
                     /product="DUF3887 domain-containing protein"
                     /protein_id="WP_143656726.1"
                     /transl_table=11
                     /translation="MFNRLGKFGVAVTVASSALVLVTGTVSHALPDTTGTAVVARPPVT
                     VPSPPPYKQLALKTLDQVVKGNFVAVSARFDESIQQEGNPAVLANAWKEYQDQLGFGRY
                     QSHGAPRQVVSGDRTVVDIPLRMAKQPGDFRVVFDGKGHIEGLWFLKKGVPVP"
     gene            complement(3963..4268)
                     /locus_tag="FNV64_RS51275"
                     /old_locus_tag="FNV64_51935"
     CDS             complement(3963..4268)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FNV64_RS51275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FNV64_51935"
                     /product="hypothetical protein"
                     /protein_id="WP_143656727.1"
                     /transl_table=11
                     /translation="MQIITVTAAVIVGALSSITYVQYAASRSPGRPIPLFSTRLALFPL
                     WADVTFTTGWIVSSFTLVFMLPIWVGVAACCGATVLPFEVMRRRHNRQVRGRVLTP"
     gene            complement(4392..4772)
                     /locus_tag="FNV64_RS51280"
                     /old_locus_tag="FNV64_51940"
     CDS             complement(4392..4772)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676862.1"
                     /locus_tag="FNV64_RS51280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_51940"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_143656728.1"
                     /transl_table=11
                     /translation="MTLEQAARGGVDAVVVYDGRLLMVAAQDSWGLPSGAPQPAESAQA
                     GAARLVYELTGYLVDGTSVLRSQGTAGEDARPAVVCQLLSQAPSAEARLGPEQIRWAPF
                     VEALDAGLPEAVRDYLVGHSPV"
     misc_feature    4804..4818
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.09"
                     /tool="antismash"
     gene            complement(5001..5798)
                     /locus_tag="FNV64_RS51285"
                     /old_locus_tag="FNV64_51945"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834463.1"
                     /locus_tag="FNV64_RS51285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_51945"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_143657259.1"
                     /sec_met_domain="Linocin_M18 (E-value: 4.1e-87, bitscore:
                     290.8, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPITPEAWAQIEEEARRTFQRNVGGRRVVDVTGPDGP
                     ALASVGTGHLTRIEGPGPDVTALLRQVQPLVELRVPFKVGRDAVDDVERGSNDSDWQPV
                     KDAARAMAFTEDRAVFEGYEAAGIDGLRRRASHPVHALPVETREYPDAVSRALTTLRLA
                     GVAGPYSLLLGADAYTLVSETSDHGYPITEHLSRMLDAPPIWAPAVDGAFLLSTRGGDF
                     ELRLGEDLAIGYTAHDANEIELYFRQSLTFLVYTDEAVVALGS"
     misc_feature    complement(5786..5804)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.86"
                     /tool="antismash"
     gene            complement(5821..6804)
                     /locus_tag="FNV64_RS51290"
                     /old_locus_tag="FNV64_51950"
     CDS             complement(5821..6804)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566630.1"
                     /locus_tag="FNV64_RS51290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_51950"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_143604560.1"
                     /transl_table=11
                     /translation="MVESQSVIAPPAKAALFLVCTMAPGGEPAVRDLLQDVAGLRRSVG
                     FRAPDAELSCVVGIGSAAWDRLFAGPRPRDLHPFVALTGSRHRAPSTPGDLLFHLRAHH
                     MDLCFELAQLIGERLYGAVAVVDEVHGFKYFDERDLLGFVDGSENPEGRVAADAVFIGD
                     EDPDFAGGSYVIVQKYVHDLAAWNALPTQEQEKVIGRTKLANVEMPDDIKPADSHVALN
                     TITDADGNERKIVRDNMPFGSIGQREFGTYFIGYARTPEVTEQMLRNMFLGDHPHTHDR
                     ILDFSTAVTGCLFHVPSADFLDDPPAAPTGASATEGSLGIGSLKGA"
     gene            complement(7083..7664)
                     /locus_tag="FNV64_RS51295"
                     /old_locus_tag="FNV64_51955"
     CDS             complement(7083..7664)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FNV64_RS51295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FNV64_51955"
                     /product="hypothetical protein"
                     /protein_id="WP_205522342.1"
                     /transl_table=11
                     /translation="MTRARWEDWGDDYERAERVSDAVGCELFSLDELYLADAPSRPSPW
                     KFAAMKAVGTPSDAEVQGLVGSPGWIVFGHNGGGDRVAADLTPGPHGHAGRIIMRDHEQ
                     DVGGDLLADSLTDLVSNGLGEGRRALAGTSTGRGPGEHRRPEQCRGRRPSRAGGPVHRR
                     VREGEAAQPRPRHRPPITRTTLEGNLGPVA"
     gene            complement(8125..8883)
                     /locus_tag="FNV64_RS51300"
                     /old_locus_tag="FNV64_51960"
     CDS             complement(8125..8883)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (2-172). E-value: 4e-10. Score:
                     31.8. Matches aSDomain:
                     nrpspksdomains_FNV64_RS51300_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545211.1"
                     /locus_tag="FNV64_RS51300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_51960"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_097288226.1"
                     /sec_met_domain="adh_short_C2 (E-value: 1.7e-30, bitscore:
                     106.1, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVAIITGASSGIGRSAAIQIARRGTGVILTYGNNAQGGLETAAA
                     IEKDGGTAVALPLDVGETGAFPAFRNSVADVLRDTWQRDTFDYLVNNAGFAHTSLIEDT
                     TEEMFDRLMRVLLKGPYFLTQRLLPLMEDGGAIVNTSSNSAMGSGLEPGYSAYASMKGG
                     LDVLSRYMAKEFSTRGIRVNSISPGSTRTRIADDAFERFPEVVPALAAKTALGRVGEPD
                     DIGMMIAALLSEESRWVTAQNIEVSGGYNL"
     aSDomain        complement(8368..8877)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FNV64_RS51300_PKS_KR.1"
                     /evalue="4.00E-10"
                     /label="FNV64_RS51300_PKS_KR.1"
                     /locus_tag="FNV64_RS51300"
                     /protein_end="172"
                     /protein_start="2"
                     /score="31.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAIITGASSGIGRSAAIQIARRGTGVILTYGNNAQGGLETAAAIE
                     KDGGTAVALPLDVGETGAFPAFRNSVADVLRDTWQRDTFDYLVNNAGFAHTSLIEDTTE
                     EMFDRLMRVLLKGPYFLTQRLLPLMEDGGAIVNTSSNSAMGSGLEPGYSAYASMKGGLD
                     VLSRYMA"
     CDS_motif       complement(8803..8871)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FNV64_RS51300_0001"
                     /evalue="3.80E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="FNV64_RS51300"
                     /protein_end="27"
                     /protein_start="4"
                     /score="13.2"
                     /tool="antismash"
                     /translation="IITGASSGIGRSAAIQIARRGTG"
     gene            complement(9009..9977)
                     /locus_tag="FNV64_RS51305"
                     /old_locus_tag="FNV64_51965"
     CDS             complement(9009..9977)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066: alpha/beta hydrolase domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056590.1"
                     /locus_tag="FNV64_RS51305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_51965"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_143656729.1"
                     /transl_table=11
                     /translation="MTLARPALDPELGALLADMPLMTHLGPELLAQLRLLPSTPVEPLL
                     EGRSVDRREVTVSGEDGARIPLSVFSPAGTAHATGAPCVFWIHGGGMVMGDRFSQIDIP
                     LEWLDLFGAVVVSVDYRLAPEVGGTTLVDDCYRGLLWIAEHADELGIDPARIVVAGASA
                     GGGLAAGVTLMARDAGALPIAAQVLICPMLDHRNVTTSSRQYSGDPGVWTREMNEFGWR
                     SVLGHLRDERVPGRVSPAVADDLSGLPTTYVDAGSAEVFRDEDVAYATRIWAAGGQAEL
                     HVWAGGFHGFDALYPQARVSAEARRTRNDWLARVLSPTSAA"
     gene            complement(10071..10388)
                     /locus_tag="FNV64_RS51310"
                     /old_locus_tag="FNV64_51970"
     CDS             complement(10071..10388)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554869.1"
                     /locus_tag="FNV64_RS51310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_51970"
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="WP_054233861.1"
                     /transl_table=11
                     /translation="MIVNHGFNATLTARPGMGDQLADLLLTGLSDGNPGTSEHCLVYLV
                     CRSASNPDVVHVTEGWTSEEDHHRVFAGEAAQAIVKRFDGLLAKDPEYTDYVPVHGKAA
                     F"
ORIGIN
        1 cagggcgtcg gcactctccc agtcggcgag gtcggcggtg ccctttccgg tgccgagcgc
       61 cgcctgcagg gcgcgtccgg atgcctcgtg acacatgttg gagcagtcgg gcagattgtt
      121 ggtgccgtac tcacgggcca tcaactggta caggaacgtc gcctcgttgc cgaggcgtcc
      181 cgaggtgtag aaggcggctt cgttcgggtc gtcgagcgcc cgcagctcgc ttccgacgag
      241 ctcgaacgcg gccttccagg tgatgggctc gtagtggtcc gtctcctcgt tgtagaccag
      301 cggctccgtc agcctgcccg tgtcctccag agcgaagtcg gtccatgtcg ccagctccga
      361 gacggagtgc gcggcgaaca gttcacgtcc ggcacgcttg ggcgtcatct cccaggtgac
      421 gtgcttgatg ccgttctcac agatgtcgag cttgagaccc ttggtgtcgt cgggccacgc
      481 gcagcccgga cagtcgaacc ccccgctctc gtggttcatc ttgaagatgg cgcgtggccc
      541 gtcgatcagc gcgtgttccc gcagcaggac gtgcgtgacg ctcttggccg cgccccagcc
      601 ggcggccgga tgatggtagg cccgctggga cggctgcttc tcggtggtgg ggccctcgcc
      661 cccggccggt gtctccgcgc cgtgcggctc gttttccgcg acagccttgt cggcatcgta
      721 ggtagtcatg ctcgtcaacc cttcgtatct gccgccgaag gagttatgtg gaaacgctac
      781 aaccatcgtg tggctctctc aatcggaccc cacggccgtc atgcggcgcc cgccctcgtc
      841 cgcgtcggtc gtatgtgtga gtcatgtggt ccgatgaagg cgctcgagca gcacataggc
      901 gcgctgctcc gcggctgcca gttgggcggg gtcgacatcc gccgcgtaca gctcgccggc
      961 ggcggcgtcg tccgcctcgc gcagttccac cactgccacg gccagctgtg tctgaacggc
     1021 gggatgcggt acggcggcgg agtcggccag tgcttgttcg gcgcgttcgg atgcttcgca
     1081 gcacgcggcc agcgcccgtt ccacacgggc tgccggacgc ccgtggacca ccagcagggc
     1141 acaggccaga ccgatgagga tgccgaggac actgcccagc gtccggtcgt ggacgagcgt
     1201 tcccacaggt gccggggacg ccaggtcgct catcagcagg gcgagcggcg tgaggaagac
     1261 gacgccgagg ccgtagttcc gggccacggt gtattccagc aggaattcga agaccacgat
     1321 cagggccacg agagccgtcg gcgccgggtg ggtggccagc accccgaacg cgatcaagag
     1381 tccggccgcc gtgcccaagg tgcgttggag ggcacgctcc aaggtggagc ggacgttgac
     1441 ggaatgcagg acggcggcag cggagagtgc ggcccagtaa ccgtgtccaa agctcaggaa
     1501 cgcggccaga ccacctgcca ggccggtgcc gagagccatc cggaacgcgg ggacgacgag
     1561 gaccgcgaca tggcccggac tgccggcacc ggtccccacg gtcagttcgg cggcgcgccg
     1621 ggccgcggga ccggcatcgt ccggacggcc gggtgtcgta tccgcggacg gggcggacgg
     1681 ggcggacggg aacggcgggt gcggcagcgg agctggggga cggcggtgcc tgacggtcag
     1741 gtaccgagcc tgcacacgca ggtaccgcgc tgtcggggcg tcgggagtct gtcggcgtgc
     1801 ggaggtgatc aggagcgacc aggcgaggtc ggtcaggaag acacatgtgc cgctccgctc
     1861 gccgtgccgc tgatcggtcg tcagggggct cagaccgagc gactggtacg cccgtaggac
     1921 ggcggcggtt gcccggtgcc gtgtgtgccc tgtgccgtcg gctcctccgc ctggttccag
     1981 caggtcggcg agctcgcgga gggccacggc caccgcgagc cgctgcgggc ggtccgggtg
     2041 cacgaaccag cccaccagtg ccagtgccca ggccgtggcc gcgcccgccg cggcgagccc
     2101 tgtctgggcg agcacgtcgg tgagcccgtc ggccgggccg ttggccgcca cggcgaacga
     2161 gaagagcatc agcacggcac cgaggccact cagtcgtgtc gcgtcgcaga ggaacttcgc
     2221 caggcccgcc gtgaccgcca tcgcggcgat caccacggcg gcgccgaggc ctcccgcccg
     2281 cggatcgatc agtgccgcga gcgtcgcgcc gaggccgacg cacatcgtca tggccaccgc
     2341 ggccacggcg aggacacgcg cacgacggcg gtagggcagg ttgcgcccga acgtggtggt
     2401 gaaagcgccc agcatgggaa agacggcgag cgcggttcgt ccggagagca ccaacggcgc
     2461 cgcgggcagt gccatggcca gcgccgcgcg cagagcgaag aggagcgctc cgtccaccgc
     2521 gtgcagggtg agcgcgccgc gcggcgagag gacgcgcgtc aatgtggtgc ggtgatggcg
     2581 ggacatacta ccgagaccgt ccgggacacc atgccaattg cgcgatccgc tcgtggaatt
     2641 gccacatctg cccacttctt cgagatcagt gaacgaggat tcctgatcag tgaacgagga
     2701 ttcctgcgaa gaggttgatg gcggtagcca gaatgctggt gccgaagacg tacgacagca
     2761 atgtgtgccg gagcaccatc gagcggatca ccgcactgga gacgtcggtg tcggagacct
     2821 ggtaggtcat gccgaggttg tagctgaagt agaagaagtc ccggtacgcc ggcggctggt
     2881 cggagttgaa atcgatcccg cccgcggaat ccgcgtagta gaggtacgcg tagcgggtgg
     2941 cgtacatcag atgcagtgcc gcccaggcca tgaacacgcc gagcagcgcc gtcgcggcat
     3001 ggccactgtc ggagttgccg agcaggaata ccacgatgcc gaccagaccg cacagagcga
     3061 cggtgacgac gacgatttcc tcgaggacgg ggcgcaggtc ctcgcgtcga gcgttgcgtc
     3121 gggtcgcggc ggcgtccatc ggccacagca cgatccagcc gaccaccacg aagaatgtct
     3181 gggtcgcggc gatacccgcg agaatggcca gcggggtgtc gctggacaat gcgacggcca
     3241 cgccgaccgc cgcgccgacg gcgaccgaga tggcgagccg tgggactgcg gaaagcgaca
     3301 aacgaatgct cacgctcaca agccacaacg gtagtgcttc gtggctgctc ggcacggcga
     3361 gaacgggacc agggcgagag cggggccagg gggtgacggt atcccgccca cggccgggcg
     3421 tacgggacgt ccccgcgtcg tggtcgtgtc acgagctcct acgggaccgg aactcctttc
     3481 ttgagaaacc acagaccttc gatatgtcct tttccgtcga agacgacgcg gaaatctccg
     3541 ggctgcttgg ccatgcgcag tggtatgtcg accaccgtcc gatctccgga cacgacctgc
     3601 cggggcgctc cgtgcgactg gtagcgaccg aaaccgagct ggtcctggta ttccttccag
     3661 gcgttcgcga gaaccgcggg atttccttct tgctgaatcg actcgtcgaa acgcgctgag
     3721 acggcgacga agtttccctt cacgacctgg tcgagggttt tgagagcgag ctgtttgtac
     3781 gggggcgggc tggggaccgt caccggtggc cgggccacga ccgcggtgcc ggtggtatcc
     3841 ggcagcgcgt gcgacacggt tccggtgacc agcaccagcg cgctggaagc gacggtgacg
     3901 gcgacgccga acttgccgag cctgttgaac atggatcctc cgactgggtg tccgtggcgg
     3961 gctcacggag tgagcacacg gcctctgacc tggcggttgt gacggcggcg catcacctcg
     4021 aaggggagca ccgtggcccc gcagcaagcg gccactccca cccagatcgg cagcatgaaa
     4081 acgagcgtga atgagctgac gatccagccc gtcgtgaacg tcacgtcggc ccatagggga
     4141 aaaagcgcga gccgggtgga gaacagaggg atcggtcgtc ctgggctgcg cgacgcggcg
     4201 tactgcacat aggtgatgct ggacaatgcc ccgacgatga cggcggccgt gacggtgatg
     4261 atctgcatga gaatctccta acctgagaaa ttctgcatcc agaaaagttc accgaggggc
     4321 ttcgcgtcat gtcacggaac agcgtgactt gaaatcagga accgatcttg ataaatgccg
     4381 cgcccgcatc cctacacggg tgaatggccc accaggtagt cccgcacggc ttcgggcagc
     4441 cccgcgtcga gggcttcgac gaacggagcc cagcggatct gttccgggcc gagtcgcgcc
     4501 tccgccgagg gggcctggct caacagctgg cacaccacgg cgggccgtgc gtcctctcca
     4561 gccgtcccct gcgacctgag gaccgatgtg ccgtcgacca ggtacccggt gagctcgtac
     4621 accaggcgcg ccgcgccggc ctgtgccgac tcggcgggct gcggggcgcc ggacggcagc
     4681 ccccagctgt cctgcgcggc caccatcagc agacgcccgt cgtacacgac gaccgcgtcc
     4741 accccgcccc gggcagcctg ctccagcgtc atggcccaac cgtccctttc ctcctcgcgc
     4801 gaccggccga ccggccccgt ccgagccccc aattcctacc cggggcacat cggttcacgc
     4861 ggcacggcga gctcgcgacc acgctcgcaa acagcggaat cgcccatcgt cacccagctg
     4921 ttccacctgc acaacgggag caggaatgac cagtgaagcc gtttcccagg gcgatccccg
     4981 tccgctcggg cgccgtgcgg tcacgagccg agtgccacca cggcctcgtc ggtgtacacc
     5041 aggaaggtca ggctctggcg gaagtacaac tcgatctcgt tcgcgtcatg cgcggtgtag
     5101 ccgatcgcga ggtcctcacc gagccgcagt tcgaagtccc caccgcgggt ggacagcaga
     5161 aaggcgccgt cgaccgcggg cgcccagatg ggcggggcgt ccagcatccg gctgagatgc
     5221 tccgtgatcg gatagccgtg gtcggacgtc tcgctcacca gggtgtacgc gtcggcgccc
     5281 agcagcagcg aatacgggcc ggcgactccg gccaggcgca gggtcgtcag cgcgcggctg
     5341 acggcatccg ggtactcacg tgtctccacg ggcagagcgt gcacaggatg ggaggcacgc
     5401 ctgcgcagcc cgtcgatgcc cgcagcctcg tacccctcga agacggcccg gtcctcggtg
     5461 aacgccatgg cgcgcgccgc gtccttgacc ggttgccagt cggagtcgtt cgagccgcgc
     5521 tccacgtcgt ccaccgcatc gcgaccgact ttgaagggga cgcgcagttc gaccaggggc
     5581 tgtacttggc gcaggagagc ggtgacgtcc ggtccgggcc cttcgatgcg ggtgagatgg
     5641 ccggtaccga ccgaggcaag cgcgggaccg tcgggtccgg tgacgtccac gacacgacgg
     5701 ccccccacgt tgcgctggaa ggtacggcgg gcttcctcct cgatctgtgc ccatgcctcg
     5761 ggcgtgatcg gcgccagttc gcggtgcagg ttgttcatac cgttgggcgt ggacacgtga
     5821 tcaggctcct ttgagactgc cgatccccag ggagccctcg gtcgccgacg ctccggtggg
     5881 cgcggcgggt ggatcgtcga ggaaatcggc gctcggtacg tggaacaggc agccggtgac
     5941 cgccgtggaa aagtcgagga ttcggtcgtg ggtatggggg tggtcaccga ggaacatgtt
     6001 cctcagcatc tgttcggtca cctcgggagt gcgcgcatag ccgatgaagt aggtaccgaa
     6061 ctccctttgc ccgatgctgc cgaacggcat gttgtcacgg acgatcttcc gttcgtttcc
     6121 gtccgcgtcg gtgatggtgt tcagtgcgac atgcgagtcc gcgggcttga tgtcgtccgg
     6181 catttcgacg ttcgccagct ttgtgcggcc gatgaccttt tcctgttcct gcgtgggcaa
     6241 cgcgttccag gcggccaggt cgtgcacgta cttctgcacg atcacgtagc tgccccccgc
     6301 gaaatcgggg tcctcgtcac cgatgaacac cgcgtcggcc gcgacccggc cctcggggtt
     6361 ctcactgcca tcgacgaagc cgaggaggtc gcgctcgtcg aagtacttga aaccgtgcac
     6421 ctcgtccacc acggcgaccg ctccgtagag gcgttcgccg atcaactggg ccagttcgaa
     6481 acaaaggtcc atgtggtggg ctcgcagatg gaagagcagg tcacctgggg tggacggggc
     6541 tcggtgccgg gagcccgtca aggcgacgaa cggatgcaga tcgcgtgggc ggggtcctgc
     6601 gaagagccgg tcccaggcgg cggatccgat gccgacgacg caactcagct ccgcgtccgg
     6661 agcgcggaaa ccgaccgatc gtcgcaaccc ggccacgtcc tggagcagat cgcgaacagc
     6721 gggctcgccg ccaggtgcca tggtgcacac gaggaacagg gcggctttgg cgggcggggc
     6781 gatcacggac tgcgactcaa ccacggagcc tgcctcctac ttgtcgtggc tatggcgcta
     6841 cgcacggcag gataacgtga ggaaacacaa taaatactag gattgggtca tggtgatgtc
     6901 gaccggtgcg ggtacaccgg cgcctgcctg cgcgaatcgc acccgacccc tggtggtcga
     6961 ggattggcgc ccccatccat tgcgccttct tcacgaggga atcctggcga tccgcaaaaa
     7021 gggtcgtaaa agggttgtgg cggaccggac ttcgaggcga agacccgccg gggcgaaccg
     7081 gtctacgcca cggggccgag gttgccttcg agggtcgtcc gagtgatcgg agggcggtga
     7141 cgggggcgag gctgagcggc ttccccctct ctcacacgcc gatggacagg acctccagct
     7201 cgggatgggc ggcggcctcg acactgctca ggcctccgat gttcacccgg gccacggccg
     7261 gtgctggtcc ccgcgagggc ccggcgtcct tcgccgagtc cgttcgacac caggtcggtg
     7321 agggagtcgg cgagcaggtc gccaccgacg tcctgctcat ggtcgcgcat gatgatccgt
     7381 ccagcgtgtc cgtgcgggcc cggtgtcagg tccgccgcga cgcggtcgcc gccgccgttg
     7441 tggccgaaga cgatccagcc gggggagccg accagtccct gcacctcggc gtcggacggg
     7501 gtgccgaccg ccttcatcgc cgcgaacttc cagggggacg gacgggacgg agcgtcggcg
     7561 aggtacagct cgtccaggga aaagagctcg cagccgaccg catcggagac gcgctccgcc
     7621 cgctcgtagt cgtcgcccca gtcctcccac cgcgcccggg tcacgcggta gagcaccttc
     7681 agctcgtcgg gtgtcctcga tctggagggc tcgtccgggc tgcgggcgcc gccgcgggag
     7741 gggcagctcg acccgtgtcg gccgatcgtc ccgccacgta gccgcccggt gcggcgatgc
     7801 tctccgcgtc ggcggttcgt agaagccgca gtacgggctc ccaggtcgcg aagtcatgta
     7861 atgagggcaa cggcggtctc cggtcgctct ggtgaaccgg cctcgcgctt tcgcgagtac
     7921 ggtacggatt gcgggtgctg atggttgctc aaagagccga ggagcgccgc cgacccggga
     7981 cgaagggacc tgtcaagggt cccgctcgtc gccgggagag ccgcgctcct caggtgagtc
     8041 agcgaatcgg gacctcctcg cgggggtgtg ctgagagcga cggccaccgc gcggtggcgc
     8101 aggccgttgg cgtgctgccc atggctacaa gttgtagcct cccgacacct cgatgttctg
     8161 agcggtgacc cagcggctct cctcggagag cagggcggcg atcatcatgc cgatgtcatc
     8221 aggttcgccg acccggccga gagcggtctt cgccgcgagg gccgggacga cttcgggaaa
     8281 ccgctcgaag gcgtcgtcgg cgatccgggt gcgcgtcgag cccggcgaaa tggaattgac
     8341 gcggatgcct cgcgtgctga actccttggc catgtagcgg ctcagcacgt cgaggccgcc
     8401 cttcatcgac gcgtaggccg agtatccggg ctccaggccc gaccccatcg ccgagttgct
     8461 gctcgtgttg acgatggccc caccgtcttc catcagcggc agcaggcgtt gcgtcaggaa
     8521 gtacgggccc ttgaggagaa cccgcatcag cctgtcgaac atctcctcgg tcgtatcctc
     8581 gatcagcgac gtgtgtgcga agccggcgtt gttgaccagg tagtcgaacg tgtcgcgctg
     8641 ccaggtgtcg cgcaacacgt cggccacgga gttgcggaag gccgggaagg cgccggtctc
     8701 accgacgtcc agcggcagcg cgacggcagt gcccccgtcc ttctcgatcg cggccgccgt
     8761 ctccaaccct ccctgggcgt tgttgccgta agtcagaatg actccggttc ctcgcctggc
     8821 gatctggatc gccgcgcttc gcccgatacc ggagctggcg ccagtgatga tggctacctt
     8881 catgatggtt ctcctggggt gtggttgagg ttccgccgag gcccggcgcg ggcctcggca
     8941 acacgggtgt cacgcgggaa atgccatgac cggccaggga gtcggccggt gtggcgtcgc
     9001 ctgctccgct atgcggcaga ggttggggac aggactcggg cgagccagtc gttgcgtgtg
     9061 cggcgggcct cggccgagac acgcgcctgc gggtacaggg cgtcgaaccc atggaagcct
     9121 cccgcccaga cgtggagttc cgcctggccg ccggccgccc agatacgggt ggcgtaggcg
     9181 acatcctcgt cgcggaagac ctcggccgag ccggcgtcga cgtaggtggt cggcaggccc
     9241 gagaggtcgt cggccaccgc gggggagaca cggccgggca cccgctcgtc gcggaggtgg
     9301 ccgagcaccg agcgccagcc gaactcgttc atctcacggg tccagacgcc gggatcgccg
     9361 gaatactgcc ggctcgaggt ggtgacattg cggtggtcga gcatggggca gatgagcacc
     9421 tgggcggcga tcggcagggc gccggcgtcg cgtgccatca gggtgacccc ggcggcgagg
     9481 ccaccgccgg cgctggcgcc cgccacgacg atgcgggcgg ggtcgatgcc caactcgtcg
     9541 gcgtgctcgg cgatccacag caacccccgg tagcagtcgt cgaccagggt cgtgccgccg
     9601 acttcgggcg ccaaccggta gtccacggag accacgacgg caccgaacag gtcgagccac
     9661 tccagcggga tgtcgatctg tgagaagcga tctcccatga ccatcccacc gccgtgtatc
     9721 cagaagacgc acggtgcccc cgtggcgtgc gcggtcccgg cggggctgaa gaccgacagg
     9781 ggaatccgcg caccgtcctc gcccgacacg gtgacctcgc gccggtcgac cgagcggcct
     9841 tcgagaaggg gctcgaccgg tgtcgacggg agcagacgca actgcgcgag cagttcgggg
     9901 ccgaggtggg tcatgagcgg catgtcggcg agcagtgcac ccagttcggg gtccaaggcg
     9961 ggtcgtgcga gagtcacggt gactaccttt cgtcgggtcg tacgggatgg tcggaggtgg
    10021 agtgcgcctg cccatcggcg tgcggaaggg ctggggatcc aacagcggcc tcagaaggcg
    10081 gccttgccgt gaaccggtac gtagtcggtg tactcggggt ccttcgccag cagcccgtcg
    10141 aaccgcttca cgatggcctg agcggcttcg ccggcgaaga cccggtggtg gtcctcctcg
    10201 ctggtccagc cctcggtgac atggacgacg tcggggttgg acgccgaacg gcagacgaga
    10261 tagacgaggc agtgttcact cgtgccgggg ttgccgtcgc tcaggccggt cagcagcagg
    10321 tcggcgagct ggtcgcccat gccgggcctg gcggtcaggg tggcgttgaa tccgtggttg
    10381 acgatcatga ctaccttctt ccgcgaggga cggatcgtgc aggtgtgctg gtcccggagc
    10441 agaagaccct ttggctctgc gctggactcc agtgactcca ttcaagcggt ctgacctgcg
    10501 gaaacggtag aacgatgctg tcggctcctt gcacaatcct cttccgtgca ggagtcggcg
    10561 acagggcgtg tgctgcaatg aaggcatgta cctcgaagag ctccgcacct tgctggctcg
    10621 acacgcacgg ccggagtgga ccaccgccat cgacggcgtc ctcatctcga aggtcgagcg
    10681 gtccgacccg ccggcgccgt cgatgaccgg cacggtgctc gcggtgatcg cacagggcgc
    10741 caaacgcctc gcgctgggcg accgggtgta cgagtaccgc gctgggcagt acctcgtc
//
