LOCUS       NZ_CP041604            10210 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. S1A1-7 chromosome.
ACCESSION   NZ_CP041604
VERSION     NZ_CP041604.2
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. S1A1-7
  ORGANISM  Streptomyces sp. S1A1-7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11713216)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Massive gene flux drive genome diversity within a population of
            Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11713216)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 11713216)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2019) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007113565.2-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:07:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,902
            CDSs (total)                      :: 10,801
            Genes (coding)                    :: 10,337
            CDSs (with protein)               :: 10,337
            Genes (RNA)                       :: 101
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 464
            CDSs (without protein)            :: 464
            Pseudo Genes (ambiguous residues) :: 0 of 464
            Pseudo Genes (frameshifted)       :: 185 of 464
            Pseudo Genes (incomplete)         :: 374 of 464
            Pseudo Genes (internal stop)      :: 46 of 464
            Pseudo Genes (multiple problems)  :: 127 of 464
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:07:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8887775
            Orig. end                         :: 8897985
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041604.2.
            On Mar 4, 2020 this sequence version replaced NZ_CP041604.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10210
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="28"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10210
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10210
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5210](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5210)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            12..326
                     /locus_tag="FNV64_RS40990"
                     /old_locus_tag="FNV64_41555"
     CDS             12..326
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025569.4"
                     /locus_tag="FNV64_RS40990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41555"
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="WP_143657171.1"
                     /transl_table=11
                     /translation="MVVRGSAECGPYRCPCCGFITLAERGVFEICTVCRWEDDGQDEHD
                     ASEVHGGPNRSLSLRQARRNFHVFGACDERGAQFARAPLPEEHPDSGTPYGIGTLYSGS
                     "
     gene            538..1119
                     /locus_tag="FNV64_RS40995"
                     /old_locus_tag="FNV64_41560"
     CDS             538..1119
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FNV64_RS40995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FNV64_41560"
                     /product="hypothetical protein"
                     /protein_id="WP_143609494.1"
                     /transl_table=11
                     /translation="MTPISTENPLTSEDAQALLAMLPTRVEIKRGMSEPELNQVEERWG
                     FRFAPEHRTLLGAGLPTGGRSWPDWRDGDAEKLAERLARPIDGVLFDVEHNGFWHPEWD
                     PRPAGTQDALEVARIYLAKVPVMVPIYSHRCLLADPNRTGTPVLSMYQTDIICYGTDLV
                     DYFHHEFGRPVPTPEDHQYAAIPFWSYFVE"
     misc_feature    1407..1420
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.76"
                     /tool="antismash"
     gene            1514..1999
                     /locus_tag="FNV64_RS41000"
                     /old_locus_tag="FNV64_41565"
     CDS             1514..1999
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /locus_tag="FNV64_RS41000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41565"
                     /product="VOC family protein"
                     /protein_id="WP_143609495.1"
                     /transl_table=11
                     /translation="MSVELTHLTLYSDDPVSLARFWAAVFTTSVTQDQGPAPRPRVVFE
                     EGRRPEVLYFEGPRAGHLSGSGAVLGIQSETGTLAQEVARLIGLGALIVVEEKGVEGLP
                     KVTMSDPGGNQFSVVPGKEEQIDWERAREAESKELTSGTATAVIIVPDEERSGNGGA"
     misc_feature    complement(2460..2474)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.24"
                     /tool="antismash"
     misc_feature    2461..2472
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.97"
                     /tool="antismash"
     gene            3018..3353
                     /locus_tag="FNV64_RS41005"
                     /old_locus_tag="FNV64_41570"
     CDS             3018..3353
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029504.1"
                     /locus_tag="FNV64_RS41005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41570"
                     /product="hypothetical protein"
                     /protein_id="WP_143609496.1"
                     /transl_table=11
                     /translation="MDAIFDLPVTSAPEQVVIARFRLSGEEFGERAERAVIFRAERAMA
                     VAVGEAGVGEVEGNEFGGGEAMVYAYGPDAGVLFKVVEPILRGLPFRPAHVVLRHGAAE
                     TQFHVDL"
     gene            complement(3556..4026)
                     /locus_tag="FNV64_RS41010"
                     /old_locus_tag="FNV64_41575"
     CDS             complement(3556..4026)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007383143.1"
                     /locus_tag="FNV64_RS41010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41575"
                     /product="hypothetical protein"
                     /protein_id="WP_143656105.1"
                     /transl_table=11
                     /translation="MDAVVQRVRTSWTKKSRGGSEAARRNAAPTVFSLPPGLSSALHEV
                     AMGEADSFEPRMQVRDLSAPGVILREVDGLLRVDPPEVTMFGMPRRNRRPPAVRLAPGQ
                     WLQWQINHRFVGSCDGAWSYQLATFNILYGPAAPDVFLSVPTRRVDERGALR"
     gene            4258..4665
                     /locus_tag="FNV64_RS41015"
                     /old_locus_tag="FNV64_41580"
     CDS             4258..4665
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020127343.1"
                     /locus_tag="FNV64_RS41015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41580"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_097286711.1"
                     /transl_table=11
                     /translation="MANGNGVPEKVAWILVRDDRVLVTRSHGRDRFYFPGGHREPGESD
                     GETLVREIDEELQAAIDPGSMVHFGTFEIGEGHPDHGPFRMICYTADHRGELTPSREIA
                     EKAWFRYADRDRVSAVDEMAFDALYEAGRLS"
     gene            complement(5001..5210)
                     /locus_tag="FNV64_RS41020"
                     /old_locus_tag="FNV64_41585"
     CDS             complement(5001..5210)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311933.1"
                     /locus_tag="FNV64_RS41020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41585"
                     /product="hypothetical protein"
                     /protein_id="WP_060897029.1"
                     /sec_met_domain="strepbact (E-value: 3e-14, bitscore: 52.5,
                     seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSAPQVQTLEISDAELDTVSGGLSPQVGMAAGPTAISSSDLVGQ
                     FGAVKDEVLDTVGQYHQVGLTVSF"
     misc_feature    5225..5240
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.09"
                     /tool="antismash"
     misc_feature    complement(5347..5360)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.35"
                     /tool="antismash"
     gene            5730..6968
                     /locus_tag="FNV64_RS41025"
                     /old_locus_tag="FNV64_41590"
     CDS             5730..6968
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431891.1"
                     /locus_tag="FNV64_RS41025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41590"
                     /product="MFS transporter"
                     /protein_id="WP_240363695.1"
                     /transl_table=11
                     /translation="MRRDRDFGVFWVAQTLSVLGDSFALIALPLLVLHATGSVARMGLL
                     TAVGGAASVVAGVFAGVVVDRVDRRKLLVTCDLVRMALYAVIPLVWVFGPRIWLLYVVL
                     PLCEAVGMLFSVAYVTVVRNLVGVERITEANGRLNATAAAAGVAGPVCAGLVAAWLGPA
                     AAVGVDAASFGVSAACVGFVRFKGTTRPAPGPVAGTGAVRRLWTDLLAGVSFLVRHPVL
                     RVLTVLLSFFSFLTLGLNDLLIYHLKHDLGRDDAAVGTVFAVGALGTIAGALLVARVRR
                     RLGFGPTWIGSVAVCGCALAGLGWARSVPAVAALTAVFLGFGSVAGTCSMSLRQEVTPE
                     HLLGRVTSAFWTLHYSAAPAGAAVLTWAAGRHGTAAVGVVAGACCVLVAVLALLTPVRR
                     PRTDGAGAPVVSR"
     gene            complement(6958..7743)
                     /locus_tag="FNV64_RS41030"
                     /old_locus_tag="FNV64_41595"
     CDS             complement(6958..7743)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063793.1"
                     /locus_tag="FNV64_RS41030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41595"
                     /product="ABC-2 family transporter protein"
                     /protein_id="WP_346268970.1"
                     /transl_table=11
                     /translation="MVWRITRLNFRAQLEYRSEFLLMIAIGAIWQVSVIVFATVLLTRF
                     AGMGGWASQDVLLIAATRMLAHGLFVLFLGRVHGLARHIQEGVIDTYLVRPMPVHRQLQ
                     LSVFPTNAIGDLTVATGLMVGALTRSQLDWNAARISYLVVCVLGGMLLEAALFTAVACA
                     SLRFPAADYWGRWLEELLGTFGSYPLNVLPRAVGGFLTFGLPLAFVAYFPAAVLTGHGG
                     DSGVPYWLAAASPLVGLLAYLGARLLWRRSLGHYSGVNG"
     gene            complement(7820..8554)
                     /locus_tag="FNV64_RS41035"
                     /old_locus_tag="FNV64_41600"
     CDS             complement(7820..8554)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005485996.1"
                     /locus_tag="FNV64_RS41035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41600"
                     /product="ABC-2 family transporter protein"
                     /protein_id="WP_143657174.1"
                     /transl_table=11
                     /translation="MTAALLRLVVQVVLVASLWRGLYATTGTTAGLTRDQAVTYAVLAV
                     LATRIRALDQYSGRDTVQQHMHFGTIVYWYLRPLPPQRYYALRALGEQLYGFLWASTGY
                     AVCLAVGFVQPPGSAAVAAVFGLSVLLGQWVLYYVMLVIDQLCFWTLRNGAAILILVFA
                     QNLLSGVYAPLWFFPDWFITLSRFLPFQATLSVPLSLYVGRIPVSQAAPQLALQAAWVV
                     AVALFTRLLWRRAARRVISQGG"
     gene            complement(8626..9621)
                     /locus_tag="FNV64_RS41040"
                     /old_locus_tag="FNV64_41605"
     CDS             complement(8626..9621)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125381.1"
                     /locus_tag="FNV64_RS41040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_41605"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_143656106.1"
                     /transl_table=11
                     /translation="MSGSIQVRGLSRTFHTTVRRPGFSGALKSLVKPERVAKEAVCDVT
                     FEVAPGELLALLGPNGAGKSTTIKMLTGILTPTSGEARVAGVVPYRERERNARNIGAVF
                     GQRTQLWWGLPVRESFAILRDIYGVPEREHRVRLGEFDELLALSSFWDTRVRHLSLGQR
                     VRCDLAAALLHDPPVVFLDEPTIGMDVVVKEQVRGFLKHQVEERGRTVLLTTHDMTEIE
                     RLAERVVLINHGRLVLDGTLDEIRRRFGSTWQVRATLADPQAAIAPPPGIALLRREGPQ
                     VVFGPDGPGAPTVHQALKQVVERYEVTDLAIDEADLEDVMRAAYVQAEAA"
ORIGIN
        1 acgtgacctt catggtggtt cgcggaagcg ccgagtgcgg gccgtaccgc tgtccctgtt
       61 gcggcttcat caccctggcc gagcgtggag tcttcgagat ctgcaccgtc tgccgctggg
      121 aggacgacgg ccaggacgag cacgacgcga gcgaggtgca cggcggcccc aatcgcagcc
      181 tcagcctgcg ccaagctcgc cggaacttcc acgtgttcgg cgcgtgcgac gagcgcggtg
      241 cccagttcgc tcgcgctccg ctgccagaag agcaccccga cagcggcaca ccctacggaa
      301 tcggcaccct ctactcgggg tcctgactgc ccgagcttga gccgcaccgc atgcgcacca
      361 gatcgagcgg ggaacagcgg ggaaccacgg tgaaagccgc cgagccgacg aggccggcca
      421 ccaatcgttc attcaggtca gcgccctacc aggcctcaaa gggacgcagc ttcccaaact
      481 gagggcacgg cgaaatcccg ctgcttgggc gtcctcaggt gccctaccgt tccgcgtatg
      541 actccgatca gcaccgagaa tccgctgacc tcagaggacg cccaggcgtt gctcgccatg
      601 ctccctaccc gagtcgagat caagcgaggc atgagtgagc ctgaactcaa ccaggtcgag
      661 gagcgctggg ggttccgctt cgctccggag caccggacgc tgttgggtgc aggactcccg
      721 acgggcggcc gtagctggcc cgactggcgg gacggcgacg cggagaagtt ggcggagcgg
      781 ctggctcggc cgatcgacgg cgtgctgttc gacgtggaac acaatggctt ctggcatccg
      841 gagtgggatc cgcggccggc gggcactcag gacgccttgg aggtggctcg catctacttg
      901 gccaaggtcc cggtgatggt gccgatctac tcgcatcggt gtctgctggc ggatccgaac
      961 cgaacgggca cgcccgtgct gtcgatgtac cagacggaca tcatttgcta cggcaccgat
     1021 ctggtcgact acttccacca cgagttcggt cgcccggtgc ccacaccgga agaccaccag
     1081 tacgccgcga tcccgttttg gtcctacttc gtggagtgaa cggctcccaa gcaggaccgg
     1141 ccttgcgccc gagggagtcc gcggcgcaca ggacgacatc cacggctgac atcaacgacg
     1201 ccggacaggg gcgtacgtca gcgactctgt ccggccgtca caccagccga cgacggacgc
     1261 cagacggggc tcgtcagagc attgatcgat taggcagccg caggtcacgg ccgccggggg
     1321 tcagttgtgg ccggcctgag gccgccgcgg tgggcgagtc ggatggttgc gtctacttcg
     1381 gcggacgtga ccggacggcg agaggctggt agcagatcaa cgtggccacg aggatccggg
     1441 cttcatacga gttctgcctc ccaggccggt acgcgaccga gggttcctga cccaagcctc
     1501 gtaaggtgtg actatgtcag tcgaactcac ccatctgacc ctctactccg acgaccccgt
     1561 gtcgctggca cgcttctggg ctgccgtttt cacgacgtcc gtaacgcaag accagggccc
     1621 tgccccccgt ccacgcgtcg tgttcgagga gggccgccga ccggaagtgc tctactttga
     1681 ggggccaagg gccggacacc tgtcaggcag cggcgccgtg ctgggaattc agtcggaaac
     1741 cgggaccttg gcgcaagagg tggcgcggct tatcggcctg ggtgccctaa tcgtcgtcga
     1801 ggaaaaaggt gtggagggcc taccaaaggt aacgatgtcc gatcctggcg ggaaccagtt
     1861 ctccgtcgta cccggcaagg aagaacaaat cgactgggaa cgtgcccgtg aagcggagag
     1921 taaggaactg acttccggta ccgcgacagc ggtcattatc gtgccagacg aggagcgatc
     1981 cggaaacggt ggcgcctgaa ctctcagccg tagggcgccg acccattagg gcggcaccct
     2041 acagccgctt gccgatcacc gcccgagagt tacgaaaaaa ggcctcgaca aaggctcatc
     2101 cgccccactg tcgaccgatt tcctgcccgg gttggttcac gtgcgtctac tcgcaatatt
     2161 cggtgtggat ggcgctttcc gcctgtgcca cgttgctcct accgtggtac tgaatgctca
     2221 ggtagaggtg agtgcggaaa tcttgccgcg caatgatgtg gatgcacttg tagccagcgg
     2281 atttcttgag gcctacgcgg caagacgtcc aacccgtcga cttgtccgcg tagacatgcc
     2341 aagcgccctg ccctggcgca cttccttcga gtgcgacgtc cagtttgcat agatccggcg
     2401 caggttcacc gcatgcagtg atttcaccct gcgcgtacag gggactgcta cttgtcgtgt
     2461 gaaaaggctt ttcggtcttc gccctgcacc agactgggag aacgtcgcgc ggcgacttgc
     2521 tgacgcccgc cacgacctca cttttaccgt cagggcgggc gtccgcgaca gcgaggccgg
     2581 ttgcacccaa gaaaagacca gcagcggcga cagcagcgaa aacgctcctg tgtaatcgat
     2641 gcactgatat ccctccacgt ctctgaggca agtcaggact tgcgacctat cgacgtcagc
     2701 gtttccgcag cgagatggag ctatgcagga accgagcatg actgtgatca cgatccgttg
     2761 atggaacctt tactgaatgt gatgcatctg agtagaaatg atgaccgtgt ctggcatcgg
     2821 gtagagcccg cttccgcacg tgcttgttga gcaaggctgg gcgtcctgct gatctacctg
     2881 cgagctcttt agggctgggg tgacctgcgc accagggtcc ttcctcatcc ccgcccttac
     2941 aaagcagagg tcgcgtgtgc gaagcggcca gggtcgatgg tcttccgatg tcagaccgcg
     3001 gtgagatcgt gtcgcgcatg gacgcgatct tcgacctccc cgtaacgagt gcccccgagc
     3061 aggttgtcat cgctcgcttc cggctgagtg gcgaagagtt cggcgagcgt gctgagcgcg
     3121 cagtgatctt ccgcgcggaa cgcgctatgg cggtcgcggt cggggaagcg ggagttgggg
     3181 aggtggaggg gaacgagttc ggtggcggcg aggccatggt gtacgcgtac gggcctgacg
     3241 ccggagtcct cttcaaggtc gtggaaccga tactgcgtgg acttccgttc aggccggcgc
     3301 atgtcgtcct gcgtcacggg gctgcggaga cacagtttca tgtggatctg tgaaggagcg
     3361 ggtacagcag cgcagtgccg caaccccaac aaccaagcca gaccgacagc gccctgcaga
     3421 gccttgaagc ggccggtatg gccgtcgtcg gccgatctgc attgagccac cggtcagaag
     3481 ctcccgccat caagagacag ctcagcgctc acccgggcca tccgtgaggc accgagagtt
     3541 tcggggcacc ggagttcagc gcagggcccc ccgctcgtcc acacggcgtg tcggcacgct
     3601 caggaacaca tcgggcgctg cggggccata aaggatgttg aatgtcgcca gttgatagga
     3661 ccaggccccg tcacaactgc cgacgaaccg gtggttgatc tgccattgca gccactggcc
     3721 gggagccagg cgaacggcgg gcgggcgccg attgcgtcgt ggcatgccaa acatggtcac
     3781 ctctggcgga tccacgcgca gcaagccgtc gacttcgcgg aggatcacgc cgggcgccga
     3841 cagatcccgc acctgcatac gaggctcgaa ggaatcggcc tctcccatag cgacctcatg
     3901 gagggcagaa ctcaagcccg gaggcagcga gaagaccgtt ggggcagcat tcctccgggc
     3961 agcttcagag ccgccgcgcg acttcttcgt ccacgacgtg cgcacccgct gaacgacagc
     4021 atccatgggc tcgatcatca agcatgacgc cgtaccgcgg ccacagattt ggataccgtg
     4081 ccagacccgc gccagatcgt gcggggaaca ccgggcacca agtcggcggt cagctggacg
     4141 ccggcaccac gccaccgcag gtcgaccggg caacccgtcc cggaacaccc cagcttccca
     4201 agctcagcgt gcggacggcg ctgacgcaaa cgaagccctc cgatacggtc ccgagccatg
     4261 gcgaatggca acggtgtgcc ggagaaggtg gcgtggatcc tggttcggga cgaccgggtg
     4321 ttggtgacgc gtagtcacgg cagagatcgc ttctacttcc cgggcggtca tcgtgagccg
     4381 ggcgagtccg acggtgagac cctggtgcgg gagatcgacg aggaactgca ggcggcgatc
     4441 gaccccggtt ccatggtgca cttcggcacg ttcgagatcg gcgagggcca tccggatcac
     4501 ggccctttcc ggatgatctg ttacacagcc gatcaccgcg gtgagctgac cccgtcgagg
     4561 gagatcgccg agaaggcgtg gttccgctac gccgatcggg accgagtctc ggccgtcgac
     4621 gagatggctt tcgacgcgct ttacgaggcc gggcggcttt cctgatccca tgaccgctgt
     4681 acagcagtgg agtacagcga gcaccacgac cgcagctgag tgatatcgct cgtaccaaga
     4741 cggcgcagcg tctgccaccc acgcagccaa cgcttcgagc gagaccttgc cgccgggggg
     4801 caaccggacg tacttcgctc gggaaagccc agctcaccaa gatccccgac aagacccagg
     4861 gaaagaagca gtgcctccga cgatcaagcc ccctaccggc gcagacccgc gggcaggggg
     4921 cttgtcgctg tggacgccga tcggcggtca ggctgctggt gtcagcgatc ccgcgatgag
     4981 gaagtcccgc gatcggacga tcagaaggag acggtcaggc cgacctggtg gtactggccg
     5041 acggtgtcca ggacctcgtc cttgaccgcg ccgaactgac ccacgaggtc cgagctactg
     5101 atcgccgtgg ggccggcggc catgccgacc tgcgggctca ggccgccgga gacggtgtcg
     5161 agctcggcgt cagagatttc aagggtctga acctggggag cggagttcat gatgaaactg
     5221 cccttcgtat ggatgttcca caagggggag cggccaggct ggggatagcc ggtgggccgc
     5281 gaccgcaggc gcacgccatc cgtttccgga cgtcttggcg cgatgccgtc ggcgcgaagg
     5341 atcaaatcac ggagggtgcc cggcatccag tcgttcgccg ctcccagcac ggcagttgga
     5401 acacttcgct actcatccat gcacgcatgc acacgaattc agggcagctt ctctacatgt
     5461 agcccggcac aggaatccga cagctattgc cacggcggct ccgatcagga caactccgta
     5521 ccaatagaac ccacccgtgc cgcagttgcg cattcgctgt gcagattcag tcacgtgtgc
     5581 atccgcgcac ccgtcccgtc taggctggtc gacgaataga tcgggtgacg ggggccgaca
     5641 cggtggcgta cagggcgcgg cgtgggagag aggccaggcc ttcggagcgg gcggcagaag
     5701 gcactgttga cgaaggcacc ccacaaccgc tgcggcggga ccgtgacttc ggtgtcttct
     5761 gggtcgcgca gaccctttcg gtcctcgggg actccttcgc gctgatcgcc ctgccgctgc
     5821 tcgtgctgca cgcgaccgga tccgtcgcgc ggatgggcct gttgacggcc gtcggcgggg
     5881 cggcctcggt cgtggccggg gtcttcgccg gggtcgtcgt cgaccgggtg gaccgtagga
     5941 agctgctggt gacctgcgat ctggtccgta tggcgctgta cgcggtgatt ccgctggtct
     6001 gggtcttcgg accgcggatc tggctgctct acgtggtcct gccgctgtgc gaggccgtcg
     6061 gcatgctgtt ctccgtcgcc tacgtcaccg tcgtccgcaa cctcgtcggc gtcgagcgga
     6121 tcacggaggc caacggcagg ctgaacgcga ccgcggcggc ggccggggtg gccggtccgg
     6181 tgtgcgcggg gctggtggcg gcgtggctcg ggccggccgc cgccgtcggc gtggacgcgg
     6241 cgagcttcgg cgtgtcggca gcctgcgtcg gcttcgtacg gttcaagggc accacacgcc
     6301 ccgcaccggg acccgtggcc ggaacgggag cggtccggcg gctgtggacg gatctgctcg
     6361 ccggggtgtc cttcctggtc cggcatccgg tgctgcgcgt gctgaccgtc ctgctgtcct
     6421 tcttcagttt cctgacactc ggcctgaacg acctgctgat ctaccacctc aagcacgatc
     6481 tcggccgcga cgacgccgcg gtcggcaccg tgttcgcggt cggcgcgctc ggcacgatcg
     6541 ccggggcgct cctggtggcc cgggtgcgcc gacgcctggg cttcggcccg acctggatcg
     6601 gttcggtggc ggtgtgcgga tgcgcgctgg cggggctggg ctgggcccgc agcgtgccgg
     6661 ccgtcgccgc cctgaccgcg gtcttcctgg gcttcgggag cgtggcgggc acgtgctcca
     6721 tgtcgctgcg gcaggaggtg actccggagc atctgctcgg acgggtcacc tcggcgttct
     6781 ggacgttgca ctactcggcg gccccggccg gcgcggccgt cctcacctgg gccgcggggc
     6841 gccacggcac ggcggcggtg ggcgtggtcg cgggcgcctg ctgcgtgctc gtcgccgtac
     6901 tggcgctgct caccccggtg cgccggcccc gtacagatgg cgccggcgca ccggtcgtca
     6961 gccgttgacc ccggagtagt ggccgaggct ccggcgccac agcagccgcg cgcccagata
     7021 ggcgagcagc cccaccagcg gtgaggccgc cgccagccag tacggcaccc ccgagtcgcc
     7081 gccgtgtccg gtcagcacgg cggccgggaa gtaggcgacg aaggccagcg gcagcccgaa
     7141 ggtgaggaaa ccgccgaccg cgcgcggcag cacgttgagc ggatagctgc cgaacgtgcc
     7201 gagcagctcc tccagccagc gcccccagta gtcggcggcc gggaaacgca gcgaggcgca
     7261 ggccaccgcc gtgaacaggg ccgcctccag gagcatcccg cccagcacac agacgacgag
     7321 gtacgagatc cgggccgcgt tccagtccag ctggctgcgg gtcagcgcgc ccaccatcag
     7381 cccggtcgcc accgtcaggt cgccgatcgc gttcgtgggg aagacggaga gctggagctg
     7441 ccggtggacc ggcatcgggc ggaccagata cgtgtcgatc acaccctcct ggatgtgccg
     7501 ggccagtccg tgcacccggc ccaggaagag cacgaacaga ccgtgcgcca gcatccgcgt
     7561 cgccgcgatc agcagcacgt cctggctcgc ccagccgccc atcccggcga accgagtgag
     7621 cagcaccgtc gcgaacacga tcaccgacac ctgccagatc gcgccgatcg cgatcatcag
     7681 caggaactcg gagcggtact ccagctgcgc gcggaagttg agacgcgtga tgcgccacac
     7741 gatcgcaggg atttcagtga tgtgcggagc ttgtccgtcg tgttcggccc gtacgtcctg
     7801 tttggtctgt ccgtcatgct cagcctccct gcgagatcac gcgccgggcg gcccggcgcc
     7861 acagcagccg ggtgaacagg gcgacggcca ccacccacgc ggcctgcagg gcgagttgag
     7921 gcgccgcctg cgacaccggg atccggccga cgtacagcga cagcggcaca ctcagcgtcg
     7981 cctggaaagg gaggaagcgg ctgagcgtga tgaaccagtc ggggaagaac cacagcggcg
     8041 cgtacacccc ggacagcagg ttctgcgcga agaccaggat caggatcgcg gcgccgttgc
     8101 ggagcgtcca gaagcacagc tggtcgatca ccagcatcac gtagtacagg acccactggc
     8161 cgagcagcac gctcagcccg aacaccgccg ccacggcggc cgacccgggc ggctggacga
     8221 acccgacggc cagacacacc gcatatccgg tcgacgccca gaggaatccg tagagttgct
     8281 cgccgagcgc ccgcaacgcg tagtagcgct gcgggggcag cgggcgcaga taccagtaca
     8341 cgatggtgcc gaagtgcatg tgctgctgga cggtgtcccg gcccgagtac tggtccaacg
     8401 ccctgatccg ggtggccagt accgcgagca cggcgtaggt gacggcctgg tcgcgggtga
     8461 ggcccgcggt ggtgccggtc gtcgcgtaca ggccgcgcca cagcgaggcc accagcacca
     8521 cctgcacgac gaggcgcagc agcgcggcgg tcatccgggg cggggtgtgc agctcgccga
     8581 gcggggtgac ccgggcggcc cgccagccgc cggggacggt cgcgctcacg cggcctccgc
     8641 ctggacgtac gccgcccgca tcacgtcctc caggtccgcc tcgtcgatcg cgaggtcggt
     8701 cacctcgtac cgctcgacga cctgcttgag cgcctggtgg accgtcgggg cgccgggccc
     8761 gtccggtccg aacaccacct gggggccctc gcggcgcagc agtgcgatgc ccggcggtgg
     8821 ggcgatcgcc gcctgtggat cggcgagcgt ggcccgcacc tgccaggtcg agccgaacct
     8881 gcgcctgatc tcgtccaggg tgccgtcgag gacgagccgc ccgtggttga tgagcacgac
     8941 ccgctcggcg agccgctcga tctccgtcat gtcgtgcgtg gtcagcagca cggtccggcc
     9001 gcgctcctcc acctggtgct tgaggaagcc gcgtacctgt tccttgacca ccacgtccat
     9061 gccgatggtg ggttcgtcga ggaacaccac gggcgggtcg tgcagcagcg cggcggcgag
     9121 gtcgcagcgc acccgctggc cgagggagag atggcgcacg cgggtgtccc agaaggagga
     9181 gagggcgagc agctcgtcga actcgccgag gcggacgcgg tgttcacgct ccggcacccc
     9241 gtagatgtcc cgcaggatcg cgaaggactc tcgcaccggc aggccccacc acagctgggt
     9301 gcgctgtccg aacaccgccc cgatgttgcg tgcgttgcgc tcgcgctccc ggtacgggac
     9361 gacgcccgcg acgcgcgcct cgccggaggt cggggtgagg atgccggtga gcatcttgat
     9421 ggtggtggac ttgccggcgc cgttcgggcc gagcagcgcg agcagttcgc cgggcgccac
     9481 ctcgaaggtg acgtcgcaga cggcctcctt cgcgacccgc tcgggcttga cgagggactt
     9541 gagcgcgccc gagaagcccg ggcggcgcac ggtggtgtga aaggtccggg acagcccccg
     9601 gacctggatg ctgccgctca tgcggacgcc ttccgggtca gtgcgatcag ccacagggtg
     9661 gccacgagcg aggccgccga cagggcggcc agcagccagg ggacggcgtg gatccccgac
     9721 gcctcgatgc ccacgccgag cagcggagga gccgccaccc cgccgatcat ggacgccgcg
     9781 atcacccagg ccccggccct cttcgcctgc gggagggccc tgttgagcca cggcagcccg
     9841 gtcgggaaga cgggcgcgat gaacagaccc acaccggcgt acgccaccgg cgccgccccg
     9901 gggatcagcg ccagcagcag gcacgcggtc atcccggccg cgcagaccgc gaggatgcgc
     9961 tcgggcgcgt accgcagggc gatcgggacg acgaggaagc ggcccgcggt catcatcagc
    10021 cagtagacgg aggtggcgga ggcggcgagc gtcgcgctgt aaccgacggt ctccaggtgg
    10081 gtgggttccc atgccgccga cgcccgcctc gacaccgacg ttcaggatgt agaggagcag
    10141 gaagcccacc aggacgcggg agagcaggcc accggaagcg gcgggccgct cggccgggga
    10201 gacgggcggc
//
