LOCUS       NZ_CP041604            11149 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. S1A1-7 chromosome.
ACCESSION   NZ_CP041604
VERSION     NZ_CP041604.2
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. S1A1-7
  ORGANISM  Streptomyces sp. S1A1-7
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 11713216)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Massive gene flux drive genome diversity within a population of
            Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11713216)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 11713216)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2019) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 58.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007113565.2-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:07:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,902
            CDSs (total)                      :: 10,801
            Genes (coding)                    :: 10,337
            CDSs (with protein)               :: 10,337
            Genes (RNA)                       :: 101
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 464
            CDSs (without protein)            :: 464
            Pseudo Genes (ambiguous residues) :: 0 of 464
            Pseudo Genes (frameshifted)       :: 185 of 464
            Pseudo Genes (incomplete)         :: 374 of 464
            Pseudo Genes (internal stop)      :: 46 of 464
            Pseudo Genes (multiple problems)  :: 127 of 464
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:07:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 817040
            Orig. end                         :: 828189
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041604.2.
            On Mar 4, 2020 this sequence version replaced NZ_CP041604.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11149
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11149
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11149
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6149](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6149
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(<813..>1004)
                     /locus_tag="FNV64_RS54825"
                     /old_locus_tag="FNV64_05410"
                     /pseudo=""
     CDS             complement(<813..1004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268373.1"
                     /locus_tag="FNV64_RS54825"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="FNV64_05410"
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MANSQLVIDGDSRRLVVVSGPFPGNRAACKAWEEPGAKAAVGNTT
                     TSADGGYPGTGTVMPHRRD"
     gene            complement(1072..1551)
                     /locus_tag="FNV64_RS05330"
                     /old_locus_tag="FNV64_05415"
     CDS             complement(1072..1551)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007495203.1"
                     /locus_tag="FNV64_RS05330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_05415"
                     /product="DUF6194 family protein"
                     /protein_id="WP_143656937.1"
                     /transl_table=11
                     /translation="MEQIIATVRGFVGALVVVPEQGGDFPELVWGDAFFYYAPDGGVPS
                     NVQPYGTIVTKNYPDDAVSDLDSPGRWRLNIHVDRATFRELTGEEPRSLTRPRDYAAAD
                     SVMPHPVYGALGWISVVNPGERTTDTAVQLLRSAHDAARARCARRHATRRSQEND"
     misc_feature    1536..1550
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.51"
                     /tool="antismash"
     gene            1599..2336
                     /locus_tag="FNV64_RS05335"
                     /old_locus_tag="FNV64_05420"
     CDS             1599..2336
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1171:
                     transcriptional regulator, MerR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554342.1"
                     /locus_tag="FNV64_RS05335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_05420"
                     /product="MerR family transcriptional regulator"
                     /protein_id="WP_143654113.1"
                     /transl_table=11
                     /translation="MGELVSGSRRVPLRTVDVAGESGYSVQQVRDLERLGVIPPAARSS
                     NGYRSYTPLHVHALRAYRGLAGAVGPVTARHMLAQLRTETIAEAASAISAVHVRLARER
                     EEALRAQQALRAIQAEANTSEFEQESDAMTITQLAGALNVRPSTLRFWEQEGLVTPERV
                     TSLQARRYGLSAIRAARIVAALRGTGYGIPAVRQIMSSLHQLDSLDKTQRILQQRLDQI
                     AARTVALLRAGADLAAVVGDHGS"
     gene            complement(2681..3955)
                     /locus_tag="FNV64_RS05340"
                     /old_locus_tag="FNV64_05430"
     CDS             complement(2681..3955)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016639005.1"
                     /locus_tag="FNV64_RS05340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_05430"
                     /product="cytochrome P450"
                     /protein_id="WP_346268876.1"
                     /sec_met_domain="p450 (E-value: 1.1e-13, bitscore: 50.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVVLPGGVGAWAVTDPAVIRGLLTDGRVSKDAYRHWPAWQRGEV
                     GAEWPLAVWVSVQNLVTSYGAEHRRLRGLMGSAFTARRVGLLGPRVGEIAAGLLEGMEA
                     AAREARGGVVDVRGLYAQPLPSRVMLELFGIPEEFRGALREIIEGFFATGVSLEDARRN
                     YAALYRTMGELVAFKRRRPGEDLTSALIAAGSGADPADPAAASDLTDLTDLTDLTDVAG
                     GVGAGGPEAPGGGGLSEKELVDNLIMLLSAGCEPTVNLLANAVVLLLREPGQLELVRSG
                     RASWGDVVEEALRVEAPGANAILRYAVEDVRVGETVIPRGDALVIGFAAAGRDPGVHGE
                     DAERFDVMRATRREHLSFGHGVHYCLGAPLARLEAEIALQALFDRFPGMRLAVEPGALR
                     PTESFISNGPREVPVLLGPPADGVR"
     misc_feature    4633..4649
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.72"
                     /tool="antismash"
     gene            5001..6149
                     /locus_tag="FNV64_RS56120"
                     /old_locus_tag="FNV64_05435"
     CDS             5001..6149
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.4"
                     /locus_tag="FNV64_RS56120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_05435"
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_143654114.1"
                     /sec_met_domain="AfsA (E-value: 9e-24, bitscore: 82.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLLTVHQDNHPTPATTATGTGTGTATGPHPAAEPDPTTRTSTET
                     KTGTGTSAKTPVDPTRMPRLTTTVPREYVHRSTLAEVFLTGCEQTGNTHYTLTAQWPRA
                     HTFFTTPDGTHHHPLQAAETIRQTGIYLAHAELGIPLGHHFLMRNLHLTTHPQHLTIGP
                     TPTDLTPHRHLHPHHPHTKRPTDFTMHITITRNGHPTATGTGRFTCLTPTTYQRLRHPP
                     TPPHQTPPTAATTTSTSTVRTQHQPPPPTHPLRTHPPPTTSSSPPPQTPTPGNSPPTPT
                     TPYSFGPHHLTTSPAWSPRSRPPSRHRPHPPPTPTPTTHPTHPTTLHATFHHYTEHHTP
                     TYITTHTTTNDNNQTPTTHITAHQNNTTVFTAQLTTHTPTHT"
     gene            complement(6424..7227)
                     /locus_tag="FNV64_RS05350"
                     /old_locus_tag="FNV64_05440"
     CDS             complement(6424..7227)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983612.1"
                     /locus_tag="FNV64_RS05350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_05440"
                     /product="VOC family protein"
                     /protein_id="WP_143654115.1"
                     /transl_table=11
                     /translation="MKSDAALHGEVTASHSVFGAPCWASLTTRDLRAAEDFYRAVMGWE
                     WRTSSTVGDQYRVASVHGVPVAGVSEVNFGVNTAVAWTPYFAVASADETVSRSQERGGT
                     TAVGPISFPPGRAALLADRDGAVFGIWEGELVAGWEEWRRAAPVFVRLHTRDAFDAAIF
                     YAEVMEWASSDPRSCEVRYEDNEVVLRSRGDVVARIHSGAVEAAPDPTIRPHWQIHFTV
                     DDVEAAARAARAHGGTAHQQGLGSTEAILTDPDGARFFVTTRTTD"
     gene            complement(7544..7717)
                     /locus_tag="FNV64_RS05355"
                     /old_locus_tag="FNV64_05445"
     CDS             complement(7544..7717)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948441.1"
                     /locus_tag="FNV64_RS05355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_05445"
                     /product="CsbD family protein"
                     /protein_id="WP_079089342.1"
                     /transl_table=11
                     /translation="MSKAKAKAKQVKGKMKETTGKAMDDESMQVEGRGEQIAGKAQETA
                     DKAAGRVKRTGR"
     misc_feature    complement(8432..8447)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.94"
                     /tool="antismash"
     gene            8557..9189
                     /locus_tag="FNV64_RS05360"
                     /old_locus_tag="FNV64_05450"
     CDS             8557..9189
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940653.1"
                     /locus_tag="FNV64_RS05360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_05450"
                     /product="DUF4360 domain-containing protein"
                     /protein_id="WP_143654116.1"
                     /transl_table=11
                     /translation="MVLRALAATGVAASLFGSSSFGGTPPPPSGRIVIDTVAVNGSGCP
                     AGAAAVTVSPDNTAFTVTYSNYLARVGPGSAPTDRKKDCRLNLRVHVPGGYTYAIVSAG
                     FSGFASLALGASGTQRASYHFQGNAHTTNSTHHFEGGFNGNWQTTDTVDVAARVFAPCG
                     EERNLDINTELRVERGTSSPSATSFIEMDSTDGRIKTVYHMAWKRCR"
     gene            complement(<9292..10000)
                     /locus_tag="FNV64_RS05365"
                     /old_locus_tag="FNV64_05455"
                     /pseudo=""
     CDS             complement(<9292..10000)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1097: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558177.1"
                     /locus_tag="FNV64_RS05365"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="FNV64_05455"
                     /product="IS5 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRLVTGVRRPEKTCHGPTRPQGAGRRRCGDREVLAAFIFVATTGC
                     TWRQLPPVFGPSGPTAHRRFTEWTGARVWAKQHRLVLDELGARGDLDCSRCAIDSVNLR
                     ALKGGR"
     misc_feature    complement(9341..9343)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(10049..10309)
                     /locus_tag="FNV64_RS05370"
                     /old_locus_tag="FNV64_05460"
     CDS             complement(10049..10309)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979487.1"
                     /locus_tag="FNV64_RS05370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV64_05460"
                     /product="hypothetical protein"
                     /protein_id="WP_143599724.1"
                     /transl_table=11
                     /translation="MSERRPEAPGTSGKPIPKPVPRDPPDQQATADVDPWDALGVPTQS
                     TAEEQPDLEIPDTDEAGTGRRGAPHSGSRRPEHPVPDEPPA"
     misc_feature    complement(10298..10309)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    complement(10504..10522)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.96"
                     /tool="antismash"
     misc_feature    complement(10714..10729)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.75"
                     /tool="antismash"
ORIGIN
        1 gctgccctcc cacccgcacg gctacacccg gccccgctac agccagttcc tcggagcact
       61 ggtcgaacac ctcggcgtcc ccgacgtgtt cctgctgggc cactcgcacg gcggcttcgt
      121 cgctcagtac cacgccctgc accaccccga gcgcatcgcc ggggtgatcc tctacgagag
      181 cgcgccgctg accggtcccg aacacggcgc cgaggccacg cgcctggtgc agtcgttcgc
      241 cgtgctccac gccgaccacc ccgaactccc cgaggtcctg gccgcgttcc aggcgatccc
      301 caccatctcc gacgacgcac agatgacagc cgtcgccaga ggggtgctcc ccgcgtactt
      361 cgccgactac tggaggcgcg aggaggagtt cgctcccatg cgtgcctcgg tgcgagccgc
      421 acacatctcc gggctggacg agaacctctc cccgcatgtc atcgacgacc gtgcggacct
      481 cggctcactc accgtgccga ccctcgtcgt cgtcggccgc cacgatgtca tctgcggggt
      541 gcgatgggcc gaggaactgc acctgctgat ccccggatcg gagctgctgg tcctggagaa
      601 cagcgggcac ttcggtcacc tcgaggagcc ggaggccttc tcgcaggcgg tgacacgttt
      661 cgttacggcc gtgaactcct ctgacagcta agccatttga tcgaagccgc aacggacggt
      721 acaacgtgag gctcgtcccg cagtgatcct ggaatcgacg cggacgaggc ggggcgctgt
      781 gtgagccgct tcgtccatcc ggagagggcg aggtcgcgcc ggtggggcat cacggtgccg
      841 gtgcccgggt agccgccgtc ggcgctcgta gtggtgttgc cgacggcggc cttggctccg
      901 ggctcttccc acgccttgca tgcggcacga ttgcctggaa acgggccgct gaccacgacg
      961 agccggcggc tgtcgccgtc gatgacgagc tgggaattgg cggagctgga gcgaactcgc
     1021 gctcaccgcg gtgtgcgggg caccctcacg tccgcttgct ccggaaccgt gtcagtcgtt
     1081 ctcctgcgac ctacgcgttg cgtgtcgccg cgcacaccgg gcacgagcgg cgtcgtgggc
     1141 gctgcgcaaa agctgcacgg ccgtgtccgt cgtcctctca ccgggattga cgacagagat
     1201 ccagccgagt gccccgtata ccgggtgcgg catcacgctg tcggccgccg cgtagtcgcg
     1261 aggtcgggtg aggctgcgcg gctcctcccc agtgagctct cggaacgtcg cacggtcgac
     1321 gtgaatgttc aggcgccagc ggcccggaga gtcaaggtcg gacacggcgt cgtcagggta
     1381 gttcttggtg acgatggtcc cgtagggctg gacgttcgaa ggtaccccac cgtcgggggc
     1441 gtagtagaag aacgcatctc cccacacgag ctccgggaaa tcgccgccct gctccgggac
     1501 aaccacgagc gcgccgacga agcctcgcac ggtcgcgatg atctgttcca tactcatggt
     1561 tcaagcatca ccttcaagtg cttatgtggg gttggctgat gggcgagctg gtgagcggtt
     1621 cgcgacgggt accgctccga accgtcgatg tcgccggaga gtcggggtac tcggtgcaac
     1681 aggttcgcga cctggaacgt ctgggagtca ttcccccagc cgctcgatcg agcaacggat
     1741 accgttccta cacaccgctg cacgtgcatg ccctacgcgc ctatcgagga ctcgcaggtg
     1801 ctgttgggcc agtcacagcc cggcacatgc tcgcgcaact gcgtacggag acgatcgcgg
     1861 aggcggcctc cgcgatcagc gcggtccatg ttcggctcgc gcgggaacga gaggaagccc
     1921 tgcgcgctca acaagcgttg cgtgcgatcc aagccgaggc gaacacatcc gagttcgagc
     1981 aggagagtga cgcgatgacg atcacacagc tcgccggagc gctcaatgta cgtccctcca
     2041 ctctgcggtt ctgggagcag gaagggctcg tcacgcccga gcgagtgacg tcactgcaag
     2101 cacgccgcta cggtctttca gccatcagag ccgcccgaat cgtcgcggcc ctgcgcggca
     2161 ccggttacgg cattcccgca gtgcgccaga tcatgagctc cctgcaccaa ctcgacagcc
     2221 tggacaagac ccaacgcatc ctgcaacaac gccttgacca gatcgccgca cggaccgtgg
     2281 ccctactccg agcgggtgca gacctggcag ccgtcgttgg cgaccacggc tcgtaggcgc
     2341 tggtcgtcag cgcggcgact acgccagatg atggagcgcc ggatcatgct gccttgctct
     2401 tcgtgcccgg cgtgatcggt gccgtcgaga gtgaagtcgc gcagggcggt gaaggagagt
     2461 agctgtcgca cacgaccgtc gggcggatcg tcgctgagtg gatggtgcat gggtagcggc
     2521 aaggtgtcgg cagttcatgc gggccgcatg tatcgcctgg tcgtgccgag agatcggcag
     2581 atgcccttcg aggccctgtt gctcgtcgcc gggagcccgt gcacgtctgc ccaagggcca
     2641 tccgccccga gcctgttcaa acagggtgca ggccggcgcg tcacctcacc ccgtcggcgg
     2701 gcggccccag caacaccggc acctcccgcg gcccgttcga aatgaacgac tccgtcggac
     2761 gcagcgcacc cggctccacc gccagccgca tcccgggaaa gcggtcgaac aacgcctgaa
     2821 gggcaatctc ggcctccagt cgcgccaacg gcgcacccag gcagtaatgc accccatgac
     2881 cgaacgacaa atgctcccgc cgcgtcgccc gcatcacgtc gaaccgctcc gcgtcctccc
     2941 cgtgcacccc cggatcccgc cccgccgccg cgaacccgat caccagcgca tccccccgcg
     3001 ggatcaccgt ctcccccacc cgcacatcct ccaccgcata ccgcaaaatc gcattcgccc
     3061 ccggcgcctc cacccgcaac gcctcctcca ccacgtcccc ccacgacgcc cgccccgacc
     3121 gcaccaactc caactgcccc ggctcccgca gcaacaacac caccgcgttc gccagcagat
     3181 tcaccgtcgg ctcacacccc gcgctcaaca acatgatcaa gttgtccacc aactccttct
     3241 cactcaaccc cccacccccc ggggcctccg ggccacccgc gcccacaccg ccagcgacgt
     3301 ctgtgaggtc tgtgaggtct gtgaggtctg tgaggtccga agcggccgcc ggatcagcgg
     3361 gatccgctcc actccccgca gcgatcagcg cactcgtcag gtcctcacct ggacgacgcc
     3421 gcttgaacgc caccaactcc cccatcgtcc gatacagcgc cgcgtagttg cgccgcgcat
     3481 cctccaacga cacccccgta gcgaaaaacc cctcaatgat ctcccgcaac gccccccgga
     3541 actcctccgg aatcccgaac aactccagca tcacccggct cggcaacggc tgcgcataca
     3601 acccccgcac gtccaccaca ccgccccgag cctcccgcgc cgccgcctcc atcccttcca
     3661 gcagccctgc cgcgatctcc cccacccgcg gacccaacaa ccccacccgc cgcgcagtga
     3721 atgccgatcc catcaacccc cgcagccggc gatgctccgc cccgtacgac gtcaccagat
     3781 tctgcaccga cacccacacc gccaggggcc actccgcccc cacctcaccc cgctgccacg
     3841 ccggccaatg ccgatacgca tccttcgaga cccgaccatc cgtcaacaac ccacggatca
     3901 ccgccggatc cgtcaccgcc caggctccca ccccacccgg caacaccacc cgcaccgccc
     3961 caccctgccc ccgcagcaac gcccctcccc tgcacatccc gccccaaccc atccatcaca
     4021 tacgggcacc caccccgccc agcacgcaga tccggcacgc cctgcgcatc cggcacatcc
     4081 tcttggtccg gcccgttcgg cacggcacgc aggtccacca cgccaccgcc gccgccccgc
     4141 aactcccgct gctcccgctg ctcctgctgc tcctgccggc cccgctcatc caaccgaccc
     4201 tgctgctcct gctgacgcgc caccggacac ctccacgaca tagaccgcga aaacttcccg
     4261 acaagcacta cacacacccc gacaccacgc ccgcaccatc acccggacgc ccaccagggg
     4321 cacaaccccg cacacacgcc aggcccagtc ccggagaacc gccccggagt cccgccatca
     4381 gccatggacc cccgacaccc gatccccgtg cgggcagcag cccgcggccc gcaggaaccc
     4441 aacccctggg cacacgcccg ggaccgggtc ccggatgcac acccggacct caccaccagc
     4501 aatcgcccag ccccacaccc ggcacaccct ggcacgcacc ctgggtgccg gtcacaggcc
     4561 gtgtgcgtcc gggtgccgac cccgggccat gcgcatcccg gaagccatcc ccccgacagc
     4621 acccacaccc gccagagaac cactcgcaaa acaaaaccgc tcggccggtt tcactatagc
     4681 ctcacccgag acccacacaa gagaccccag cacaccccca caaccacccg accccgagcc
     4741 tcgcacacca cccccaaccc ccggaagaca cccgcagaac ggaatcaaaa ccaccgctta
     4801 tccagcgaag atccccacca cacacccaca gcgaaccaat gaatatgcac ccctgcacac
     4861 ccccacccga cccccggacc aacccccggc ccgcccccgc gaaaatcccc gcccgccctt
     4921 gaaaagaaaa ccggtaggtt cgtatcttat gaccctgcac ggtccatccc ggcacctccc
     4981 acagcgccac ggagacccac atgaccctgc tcaccgtcca ccaggacaac caccccaccc
     5041 ccgccaccac ggcaacggga acgggaacgg gaacggcaac aggcccgcac ccggcagcag
     5101 aaccagaccc caccacgcgg accagcacgg aaacaaaaac aggcaccggc accagcgcaa
     5161 aaacacccgt cgaccccacc cgcatgcccc gcctcaccac caccgtcccc cgcgaatacg
     5221 tccaccgctc caccctcgcc gaggtcttcc tcaccggctg cgaacaaacc ggcaacaccc
     5281 actacaccct caccgcccaa tggccccgcg cccacacctt cttcaccacc cccgacggca
     5341 cccaccacca ccccctccaa gccgcagaaa ccatccgcca aaccggcatc tacctcgccc
     5401 acgccgaact cggcatcccc ctcggccacc acttcctcat gcgcaacctc cacctcacca
     5461 cccaccccca acacctcacc atcggcccca cccccaccga cctcacccct caccgccacc
     5521 tacaccccca ccacccccac accaaacgcc ccaccgactt caccatgcac atcaccatca
     5581 cccgaaacgg ccaccccact gccaccggca ccggccgctt cacctgcctc acccccacca
     5641 cctaccaacg cctccgccac ccccccacac caccacacca gacaccaccc acagcagcca
     5701 ccaccaccag caccagcacc gtccgcaccc aacaccaacc cccacccccc acccacccac
     5761 tacggacgca cccccccccc acgacatcgt cctcaccccc accccaaacc cccacacctg
     5821 gcaactcacc cccaaccccc accaccccat actctttcgg accacaccac ctgaccacat
     5881 ccccggcatg gtccccccga agccgcccac caagccgcca ccgcccacac ccacccccca
     5941 cccccacccc cacaacccac cccacccacc ccaccaccct ccacgccacc ttccaccact
     6001 acaccgaaca ccacaccccc acctacatca ccacccacac caccaccaac gacaacaacc
     6061 aaacccccac cacccacatc accgcacacc aaaacaacac caccgtcttc accgcacaac
     6121 tcaccaccca cacccccacc cacacctgag aacttctaac gagacaggag ctcagcttcg
     6181 gttcgggcat ggccggagtc cggtgtggct ccactggccg ggcatgctgc ttgtacagat
     6241 gctcacgcgg cgccgatgga ctgctgaggc aatgcgcaac ttcctgcgtc atgccacgac
     6301 cgcgagccga ccgcgctcgt gacccggctc ccggaggtca ccgaacggca cgtcgccgac
     6361 caggccctca agttggagac cgttcagcac atcctggacc gggcagcact gacgctgcgg
     6421 ccctcagtcc gtcgttcgcg tggtcacgaa gaagcgggct ccgtcggggt cggtgaggat
     6481 ggcctcggtc gagccgaggc cctgctggtg ggccgtgccg ccgtgggcgc gggcggcgcg
     6541 ggcggcggcc tcgacgtcgt cgacggtgaa gtggatctgc cagtgcggcc ggatcgtcgg
     6601 gtcgggtgcg gcctcgaccg cgccggagtg gatacgggcc acgacgtcgc cccggctgcg
     6661 cagcacgacc tcgttgtcct cgtagcgcac ctcgcaggac cgggggtccg aggacgccca
     6721 ctccatgacc tccgcgtaga agatggcggc gtcgaaggcg tcgcgggtgt gcagccgtac
     6781 gaagaccggt gccgcccggc gccactcctc ccagccggcc accagctcgc cctcccagat
     6841 gccgaacacc gctccgtccc ggtcggccag cagggccgcc cggcccggcg ggaacgagat
     6901 cggccccacc gcggtcgtac ccccgcgctc ctggctacgg gacacggtct cgtccgcact
     6961 cgccaccgcg aaatacgggg tccacgcgac cgccgtgttc accccgaaat tcacctccga
     7021 aacccctgcg accgggaccc cgtgcacgct cgcgacgcgg tactggtcgc ccaccgtgct
     7081 gctcgtgcgc cactcccagc ccatcacggc acggtagaaa tcctccgccg cccgcagatc
     7141 gcgcgtggtc agactcgccc agcagggtgc cccgaacacc gagtgactgg cggtgacctc
     7201 accgtgcagt gctgcgtcgc tcttcatcct cgtaccgcct gacctacctg acaccacagc
     7261 catcaagggg cgttgaccta ccccggaccc agtgctcgcc caaaagccga gtgccgaacg
     7321 tacggcaacc cgctgctccg cgttcaccgc gcagcgaccg ccgcgagaca cgtcgcactc
     7381 gctgccgtgg cgcgcaacag accgaggcgg tcggggctgt cccggccgcc tcgaatcggc
     7441 tcacgcagcc aaaccgcagg tggtgccgac cgtcgagatc ttcggactcg gcgttcgctc
     7501 ggctcggtgc gtcccgtcga atgtgtcgcc ggagcgagcg gtcctaccgg ccggtcctct
     7561 tcacacggcc cgcagccttg tccgcggtct cctgcgcctt accggcgatc tgctcaccac
     7621 ggccctcgac ctgcatgctc tcgtcgtcca tggccttgcc agtggtctct ttcatctttc
     7681 ctttgacctg cttggccttt gccttcgcct tgctcatgat ccgttgccct ccaatgaggc
     7741 tcgaattggt gcttaaacca tgcgctgggc acgacggctc cgcaaccgag ccacaatgcg
     7801 gtcgcgcagc cgtgtccgcg aaatgcggca gcaccaagga atgccagaac gaaacagatc
     7861 cagggcgcga tgagggaggc ccctcggcaa agtccgtggg ttaccgatca tgcagctgcc
     7921 aaggcggcga gcagcttcgt ggcatggccc gagagggaca gggcggaggc attctcgccc
     7981 tgcggatatg ggtcacgtcc gttggagtgg tgtatcgacg gccactcggt gatctccttt
     8041 tgacgctgtg cagtgagttg gacgtcgtct caattggtcc tgcgaagagc cgggcgtctc
     8101 gggtggagcg ggcgcttcag cgcacgcaca atgtgacctg gaggacgggc cgcagttgcg
     8161 gaagaagcag cggcgcaagg agtcccggct cgatccctac aagtcgttgt tcgacggcat
     8221 gctgcgggct gactggatgc tcgtcccata cagcggcaca ccacccagcg catctatgag
     8281 cgcctgatgg agagccaccg gcttgtcacc ctcggcgcgc cccctgggtg atggcggtag
     8341 agcagattga gtgtgccggt gtatccgagc tcgcgcgccg ctctgttccc gtgagtggat
     8401 gaacgaggcc tcccccctga aggccgttcg agtgactgga gcgtcacctt ccgtccgcgg
     8461 acacgcaacg tgtggtgcga tgcccacgtg aaatccgggg gccgggctcg gaaagtgacc
     8521 ccggccctgg atccgtggac gattgaaggg aagcccatgg tgctccgcgc gctggccgcg
     8581 actggtgttg ccgcatcatt gttcggctcg tcgtccttcg gcggcacccc gcccccgccg
     8641 tcgggccgga tcgtcatcga cacggtggcg gtcaacggtt ccggctgccc ggcgggggcg
     8701 gccgccgtga ccgtgtcccc tgacaacacc gccttcaccg tcacctacag caactacctc
     8761 gcccgggtgg ggcccggctc ggcgccgacc gaccggaaga aggactgtcg gctcaacctg
     8821 cgagtgcacg tccccggtgg ctacacctac gcgatcgtgt ccgccggctt cagcggcttc
     8881 gcgtcactgg cgctgggtgc gtccgggact cagcgggcca gctaccactt ccagggcaac
     8941 gcccacacca ccaactcgac ccatcacttc gagggcgggt tcaacggcaa ctggcagacg
     9001 accgacactg tggacgtcgc cgcccgggtc ttcgccccct gcggggagga gcgcaacctc
     9061 gacatcaaca ccgaactgcg ggtcgagcgc ggcacctcga gcccgagcgc caccagcttc
     9121 atcgagatgg actcaacgga cggcaggatc aaaaccgtgt accacatggc ctggaagcgg
     9181 tgccgatgat ctcctggcgc tgcaatcggc gggtgcgtcc aggccaacgg ctctcgtcat
     9241 cggtggtagc actgtgtggt gatcaccgga ggagaatgcg ctggcgaagc agctgcaggc
     9301 aatggcggtg aaggccagga agtgctcggc cttgcgttcg taacggcggt gcaggcggcg
     9361 gcagccgccc agccaggaca tcgtgcgctc gatcgtccgg cggtgtcggg ccagccgcgt
     9421 ggaggactcg atgcccttgc gggcgatgcg gtggtggatg ccgcgctcac gaagccatcg
     9481 gcgccggtgg tcgtagtcgt agcccttgtc gccatggagc ttggcagggc gtcggcggcg
     9541 cgggcggcga cgggagcgta tcggcgggag cccctgcacc agggcttgga gggcctggct
     9601 gtcgtggagg ttggcgtcgg agatgccgat cgagaggggc ggaccctgtc gacaggatcc
     9661 ggacctgtca gcgcccccct ttcagggccc gcaggttcac cgagtcgatc gcgcagcgcg
     9721 agcagtccaa gtccccgcgg gcgccgagct cgtccaggac caggcggtgc tgcttggccc
     9781 acacgcgggc gccggtccac tcggtgaagc gccggtgtgc ggtcgggccc gaggggccga
     9841 acacgggagg cagttggcgc caggtgcagc ccgtcgtggc cacgaagatg aacgcggcca
     9901 ggacttcccg gtcgccgcag cgtcgccggc cggcgccctg tggccgcgtc gggccatgac
     9961 atgtcttctc gggccgccgg actcctgtca ccagtcgcag gtatgtctcc cagcgttacc
    10021 tgccggggct cccgcggcac gcggagcgtc aggcgggcgg ttcgtcgggg acgggatgct
    10081 ccggacgtcg gctgccggag tgcggggcgc ccctgcggcc ggtgccggcc tcgtccgtat
    10141 cgggaatctc caggtctggc tgctcctcgg cggtggactg cgtcgggacg ccgagggcgt
    10201 cccaggggtc cacgtcggcg gtggcctgct gatcgggcgg atccctgggt accggcttcg
    10261 gtatcggctt cccactggtc ccaggggctt ccgggcggcg ttcactcacg gtgttctcgc
    10321 ttcctcgatc aggggggtgg tgtgagtcgc aggtaccccg gcggccgacg atgatgccga
    10381 tgatcgaggc cccgccccga ggctggtcgg cggctgcgcg gagggttgac gagttccccc
    10441 accgtcgctc cgatcacaga aggggacaca gcaaccgccg gaattccgaa cgatggtgct
    10501 gcaatggagc gtcgatcgcc atcgtccagg cgtcacaact cgtcggcatc gtaaggtgtc
    10561 ccctgtgaaa ctccaggagt tgctcgacaa gcaagactcc tatggtgccc cggctgtgtt
    10621 agcaggactt tccgcagcca aggtacgaag tgtcgctact cgtggggcag ttcgggtgag
    10681 cccggactcc tgctttcgca tcgcgtcgtt gacaaaggtc ttcacctgta ctgcgctcgt
    10741 ggagaccatg cgagacaact ccatccctct cggtacagcc gtcatcgagt tgcttcctga
    10801 cctcgcacct ggctgccgag ccgatgtgcg cctcgccctc gagcagatcc tcgggcaggt
    10861 ctcggggatg cgcgagtctg tgggctcgac cgccctggcc gctctgggcg acggaccgcg
    10921 tgcgctccaa gaggcggccg agctcgtcgt gggcacgggg aacgaacacg agcccggggc
    10981 tcgctggtcc tccggcaact acctcctggc cggtgcggtc ctcgccgccc tcagtggcac
    11041 ttcgtacgag agggcggtgg agaagacgct gcttggcccg tggaatggtc tacggtctcg
    11101 gatgggcgct cagcccttca ggccagacgt gtctcaacgg acggctgcc
//
