LOCUS       NZ_CP041610            10798 bp    DNA     linear   CON 14-NOV-2023
DEFINITION  Streptomyces sp. RLB3-17 chromosome, complete genome.
ACCESSION   NZ_CP041610
VERSION     NZ_CP041610.2
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. RLB3-17
  ORGANISM  Streptomyces sp. RLB3-17
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 12022941)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Massive gene flux drive genome diversity within a population of
            Streptomyces
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12022941)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-FEB-2020) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 12022941)
  AUTHORS   Tidjani,A.-R., Lorenzi,J.-N., Toussaint,M., Van Dijk,E., Naquin,D.,
            Lespinet,O., Bontemps,C. and Leblond,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2019) Dynamique des Genomes et Adaptation
            microbienne UMR 1128, Universite de Lorraine - Institut National de
            la Recherche Agronomique, Faculte des Sciences et Technologies
            Boulevard des Aiguillettes BP 70239, Vandoeuvre-les-Nancy F-54506,
            France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66.0x
            Sequencing Technology             :: Illumina MiSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/14/2023 04:19:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11,027
            CDSs (total)                      :: 10,924
            Genes (coding)                    :: 10,421
            CDSs (with protein)               :: 10,421
            Genes (RNA)                       :: 103
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 503
            CDSs (without protein)            :: 503
            Pseudo Genes (ambiguous residues) :: 0 of 503
            Pseudo Genes (frameshifted)       :: 178 of 503
            Pseudo Genes (incomplete)         :: 409 of 503
            Pseudo Genes (internal stop)      :: 52 of 503
            Pseudo Genes (multiple problems)  :: 128 of 503
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:01:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 11022870
            Orig. end                         :: 11033668
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041610.2.
            On Mar 4, 2020 this sequence version replaced NZ_CP041610.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="42"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5798)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(780..796)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.97"
                     /tool="antismash"
     gene            complement(879..2603)
                     /locus_tag="FNV62_RS49625"
                     /old_locus_tag="FNV62_50310"
     CDS             complement(879..2603)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018468799.1"
                     /locus_tag="FNV62_RS49625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV62_50310"
                     /product="FUSC family protein"
                     /protein_id="WP_143629965.1"
                     /transl_table=11
                     /translation="MSRHHRTTLTRVLSPRGALTLHAVDGALLFALRAALAMALPAAPL
                     VLSGRTALAVFPMLGAFTTTFGRNLPYRRRARVLAVAAVAMTMCVGLGATLAALIDPRA
                     GGLGAAVVIAAMAVTAGLAKFLCDATRLSGLGAVLMLFSFAVAANGPADGLTDVLAQTG
                     LAATGAATAWALALVGWFVHPDRPQRLAVAVALRELADLLEPGGGADGTGHTRHRATAA
                     VLRAYQSLGLSPLTTDQRHGERTGTCVFLTDLAWSLLITSARRQTPDAPTARYLRVQAR
                     YLTVRHRRPPAPLPHPPFRCAPSAPSADTAPGRPDDAGPAARRAAELTVGTGAGSPGHV
                     AVLVVPAFRMALGTGLAGGLAAFLSFGHGYWAALSAAAVLHSVNVRSTLERALQRTLGT
                     AAGLLIAFGVLATHPAPTALVALIVVFEFLLEYTVARNYGLGVVFLTPLALLMSDLASP
                     APVGTLVHDRTLGSVLGILIGLACALLVVHGRPAARVERALAACGEASERAEQALADSA
                     AVPHPAVQTQLAVAVVELREADDAAAGELYAADVDPAQLAAAEQRAYVLLERLHRTT"
     gene            complement(2704..3330)
                     /locus_tag="FNV62_RS49630"
                     /old_locus_tag="FNV62_50315"
     CDS             complement(2704..3330)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952221.1"
                     /locus_tag="FNV62_RS49630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV62_50315"
                     /product="DUF1345 domain-containing protein"
                     /protein_id="WP_143629967.1"
                     /transl_table=11
                     /translation="MSIRLSLSAVPRLAISVAVGAAVGVAVALSSDTPLAILAGIAATQ
                     TFFVVVGWIVLWPMDAAATRRNTRREDLRPVLEEIVVVTVALCGLVGIVVFLLGNSDSG
                     HAATALLGVFMAWAALHLMYATRYAYLYYADSAGGIDFNSDQPPAYRDFFYFSYNLGMT
                     YQVSDTDVSSAVIRSMVLRHTLLSYVFGTSILATAINLFAGILVH"
     gene            complement(3459..3932)
                     /locus_tag="FNV62_RS49635"
                     /old_locus_tag="FNV62_50320"
     CDS             complement(3459..3932)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FNV62_RS49635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FNV62_50320"
                     /product="DUF3887 domain-containing protein"
                     /protein_id="WP_143629969.1"
                     /transl_table=11
                     /translation="MFNRLGNFGVAVTVASSALMLVTGTVSHALPDTTGTAVVARPPVT
                     VPSPPPYKQLALKTLDQVVKGNFVAVSARFDESIRQEGNPVVLSNAWKEYQDQLGFGRY
                     QSHGAPRQVVSGDRTVVDIPLRMAKQPGDFRVVFDGKGHIEGLWFLKKGVPVP"
     gene            complement(3963..4268)
                     /locus_tag="FNV62_RS49640"
                     /old_locus_tag="FNV62_50325"
     CDS             complement(3963..4268)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FNV62_RS49640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FNV62_50325"
                     /product="hypothetical protein"
                     /protein_id="WP_143629971.1"
                     /transl_table=11
                     /translation="MQIITVTAAVIVGALSSTTYVQYAASRSPGRPIPLFSTRLALFPL
                     WADVTFTTGWIVSSFTLVFMLPIWVGVAACCGATVLPFEVMRRRHNRQVRGRVLTP"
     gene            complement(4392..4772)
                     /locus_tag="FNV62_RS49645"
                     /old_locus_tag="FNV62_50330"
     CDS             complement(4392..4772)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676862.1"
                     /locus_tag="FNV62_RS49645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV62_50330"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_060898592.1"
                     /transl_table=11
                     /translation="MTLEQAARGGVDAVVVYDGRLLMVAAQDSWGLPSGAPQPAESAQA
                     GAARLVYELTGYLVDGTSVLRSQGTAGEDARPAVVCQLLSQAPSAEARLGPEQIRWAPF
                     AEALDAGLPEAVRDYLVGHSPV"
     misc_feature    4804..4818
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.09"
                     /tool="antismash"
     gene            complement(5001..5798)
                     /locus_tag="FNV62_RS49650"
                     /old_locus_tag="FNV62_50335"
     CDS             complement(5001..5798)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834463.1"
                     /locus_tag="FNV62_RS49650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV62_50335"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_143652438.1"
                     /sec_met_domain="Linocin_M18 (E-value: 6.7e-87, bitscore:
                     290.1, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPITPEAWAQIEEEARRTFQRNVGGRRVVDVTGPDGP
                     ALASVGTGHLTRIEGPGPDVTALLRQVQPLVELRVPFKVGRDAVDDVERGSNDSDWQPV
                     KDAARAMAFTEDRAVFEGYEAAGIDGLRRRASHPVHALPVETRAYPDAVSRALTTLRLA
                     GVAGPYSLLLGADAYTLVSETSDHGYPITEHLSRMLDAPPIWAPAVDGAFLLSTRGGDF
                     ELRLGEDLAIGYTAHDANEIELYFRQSLTFLVYTDEAVVALGS"
     misc_feature    complement(5786..5804)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.86"
                     /tool="antismash"
     gene            complement(5821..6804)
                     /locus_tag="FNV62_RS49655"
                     /old_locus_tag="FNV62_50340"
     CDS             complement(5821..6804)
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566630.1"
                     /locus_tag="FNV62_RS49655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV62_50340"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_143604560.1"
                     /transl_table=11
                     /translation="MVESQSVIAPPAKAALFLVCTMAPGGEPAVRDLLQDVAGLRRSVG
                     FRAPDAELSCVVGIGSAAWDRLFAGPRPRDLHPFVALTGSRHRAPSTPGDLLFHLRAHH
                     MDLCFELAQLIGERLYGAVAVVDEVHGFKYFDERDLLGFVDGSENPEGRVAADAVFIGD
                     EDPDFAGGSYVIVQKYVHDLAAWNALPTQEQEKVIGRTKLANVEMPDDIKPADSHVALN
                     TITDADGNERKIVRDNMPFGSIGQREFGTYFIGYARTPEVTEQMLRNMFLGDHPHTHDR
                     ILDFSTAVTGCLFHVPSADFLDDPPAAPTGASATEGSLGIGSLKGA"
     misc_feature    complement(7040..7055)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.08"
                     /tool="antismash"
     gene            complement(8049..8807)
                     /locus_tag="FNV62_RS49660"
                     /old_locus_tag="FNV62_50350"
     CDS             complement(8049..8807)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (2-172). E-value: 4.9e-10. Score:
                     31.6. Matches aSDomain:
                     nrpspksdomains_FNV62_RS49660_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545211.1"
                     /locus_tag="FNV62_RS49660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV62_50350"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_143629973.1"
                     /sec_met_domain="adh_short_C2 (E-value: 2.4e-30, bitscore:
                     105.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVAIITGASSGIGRSAAIQIARRGTGVILTYGNNSQGGLETAAA
                     IEKDGGTAVALPLDVGETGAFPAFRNSVADVLRDTWQRDTFDYLVNNAGFAHTSLIEDT
                     TEEMFDRLMRVLLKGPYFLTQRLLPLMEDGGAIVNTSSNSAMGSGLEPGYSAYASMKGG
                     LDVLSRYMAKEFSTRGIRVNSISPGSTRTRIADDAFERFPEVVPALAAKTALGRVGEPD
                     DIGMMIAALLSEESRWVTAQNIEVSGGYNL"
     aSDomain        complement(8292..8801)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FNV62_RS49660_PKS_KR.1"
                     /evalue="4.90E-10"
                     /label="FNV62_RS49660_PKS_KR.1"
                     /locus_tag="FNV62_RS49660"
                     /protein_end="172"
                     /protein_start="2"
                     /score="31.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAIITGASSGIGRSAAIQIARRGTGVILTYGNNSQGGLETAAAIE
                     KDGGTAVALPLDVGETGAFPAFRNSVADVLRDTWQRDTFDYLVNNAGFAHTSLIEDTTE
                     EMFDRLMRVLLKGPYFLTQRLLPLMEDGGAIVNTSSNSAMGSGLEPGYSAYASMKGGLD
                     VLSRYMA"
     CDS_motif       complement(8727..8795)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FNV62_RS49660_0001"
                     /evalue="3.80E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="FNV62_RS49660"
                     /protein_end="27"
                     /protein_start="4"
                     /score="13.2"
                     /tool="antismash"
                     /translation="IITGASSGIGRSAAIQIARRGTG"
     gene            complement(8933..9901)
                     /locus_tag="FNV62_RS49665"
                     /old_locus_tag="FNV62_50355"
     CDS             complement(8933..9901)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066: alpha/beta hydrolase domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056590.1"
                     /locus_tag="FNV62_RS49665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV62_50355"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_143629975.1"
                     /transl_table=11
                     /translation="MTLARPALDPELGALLADMPLMTHLGPELLAQLRLLPSTPVEPLL
                     EGRSVDRREVTVSGEDGARIPLSVFSPAGTAHATGAPCVFWIHGGGMVMGDRFSQIDIP
                     LEWLDLFGAVVVSVDYRLAPEVGGTTLVDDCYRGLLWIAEHADELGIDPARIVVAGASA
                     GGGLAAGVTLMARDAGTLPIAAQVLICPMLDHRNVTISSRQYSGDPGVWTREMNEFGWR
                     SVLGHLRDERVPGHVSPAVADDLSGLPTTYVDAGSAEVFRDEDVAYATRIWAAGGQAEL
                     HVWAGGFHGFDALYPQARVSAEARRTRNDWLARVLSPTSAA"
     gene            complement(9995..10312)
                     /locus_tag="FNV62_RS49670"
                     /old_locus_tag="FNV62_50360"
     CDS             complement(9995..10312)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554869.1"
                     /locus_tag="FNV62_RS49670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FNV62_50360"
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="WP_054233861.1"
                     /transl_table=11
                     /translation="MIVNHGFNATLTARPGMGDQLADLLLTGLSDGNPGTSEHCLVYLV
                     CRSASNPDVVHVTEGWTSEEDHHRVFAGEAAQAIVKRFDGLLAKDPEYTDYVPVHGKAA
                     F"
ORIGIN
        1 acgcccatga tgaacagggc gtcggcactc tcccagtcgg cgaggtcggc ggtgcccttt
       61 ccggtgccga gcgacgcctg cagggcgcgt ccggacgcct cgtgacacat gttggagcag
      121 tcgggcagat tgttggtgcc gtactcacgg gccatcaact ggtacaggaa cgtcgcctcg
      181 ttgccgaggc gtcccgaggt gtagaaggcg gcttcgttcg ggtcgtcgag ggcccgcagc
      241 tcgcttccga cgagctcgaa cgcggccttc caggtgatgg gctcgtagtg gtccgtctcc
      301 tcgttgtaga ccagcggctc cgtcagcctg cccgtgtcct ccagagcgaa gtcggtccat
      361 gtcgccagct ccgagacgga gtgcgcggcg aagagctcac gtccggcacg cttgggcgtc
      421 atctcccagg tgacgtgctt gatgccgttc tcacagatgt cgagcttgag acccttggtg
      481 tcgtcgggcc acgcgcagcc cgggcagtcg aaccccccgc tctcgtggtt catcttgaag
      541 atggcgcgtg gcccgtcgat cagcgcgtgt tcccgcagca ggacgtgcgt gacgctcttg
      601 gccgcacccc agccggcggc cggatgatgg taggcccgct gggacggctg cttctcggtg
      661 gtggggccct cgcccccggc cggtgtctcc gcgccgtgcg gctcgttttc cgcgacagcc
      721 ttgtcggcat cgtaggtagt catgctcgtc aacccttcgt atctgccgcc gaaggagtta
      781 tgtggaaacg ctacaaccat cgtgtggctc tctcaatcgg accccacggt cgtcatgcgg
      841 cgcccgcccc tcgtccgcgt cggtcgtatg tgggggattc atgtggtccg atgaaggcgc
      901 tcgagcagca cataggcgcg ctgctccgcg gctgccagtt gggcggggtc gacatccgcc
      961 gcgtacagct cgccggcggc ggcgtcgtcc gcctcgcgca gttccaccac tgccacggcc
     1021 agctgtgtct gaacggcggg atgcggtacg gcggcggagt cggccagtgc ttgttcggcg
     1081 cgttcggatg cttcgccgca cgcggccagc gcccgttcca cacgggctgc cggacgcccg
     1141 tggaccacca gcagggcaca ggccagaccg atgaggatgc cgaggacact gcccagcgtc
     1201 cggtcgtgga cgagcgttcc cacaggtgcc ggggacgcca ggtcgctcat cagcagggcg
     1261 agcggcgtga ggaagacgac gccgaggccg tagttccggg ccacggtgta ttccagcagg
     1321 aattcgaaga ccacgatcag ggccacgaga gccgtcggcg ccgggtgggt ggccagcacc
     1381 ccgaacgcga tcaagagtcc ggccgccgtg cccaaggtgc gttggagggc acgctccaag
     1441 gtggagcgga cgttgacgga atgcaggacg gcggcagcgg agagtgcggc ccagtaaccg
     1501 tgtccaaagc tcaggaacgc ggccagacca cctgccaggc cggtgccgag agccatccgg
     1561 aacgcgggga cgacgaggac cgcgacatgg cccggactgc cggcaccggt ccccacggtc
     1621 agttcggcgg cgcgccgggc cgcgggaccg gcatcgtccg gacggccggg tgccgtatct
     1681 gcggacgggg cggacggggc gcaccggaac ggcgggtgcg gcagcggagc tgggggacgg
     1741 cggtgcctga cggtcaggta ccgagcctgc acacgcaggt accgcgctgt cggggcgtcg
     1801 ggagtctgtc ggcgtgcgga ggtgatcagg agcgaccagg cgaggtcggt caggaagaca
     1861 catgtcccgg tccgctcgcc gtgccgctga tcggtcgtca gggggctcag accgagcgac
     1921 tggtacgccc gtaggacggc ggcggttgcc cggtgccgtg tgtgccctgt gccgtcggct
     1981 cctccgcctg gttccagcag gtcggcgagc tcgcggaggg ccacggccac cgcgagccgc
     2041 tgcgggcggt ccgggtgcac gaaccagccc accagtgcca gtgcccaggc cgtggccgcg
     2101 cccgtcgcgg cgagccctgt ctgggcgagc acgtcggtga gcccgtcggc cgggccgttg
     2161 gccgccacgg cgaacgagaa gagcatcagc acggcaccga ggccactcag tcgtgtcgcg
     2221 tcgcagagga acttcgccag gcccgccgtg accgccatcg cggcgatcac cacggcggcg
     2281 ccgaggcctc ccgcccgcgg atcgatcagt gccgcgagcg tcgcgccgag gccgacgcac
     2341 atcgtcatgg ccaccgcggc cacggcgagg acacgcgcac gacggcggta gggcaggttg
     2401 cgcccgaacg tggtggtgaa agcgcccagc atgggaaaga cggcgagcgc ggttcgtccg
     2461 gagagcacca acggcgccgc gggcagtgcc atggccagcg ccgcgcgcag agcgaagagg
     2521 agcgctccgt ccaccgcgtg cagggtgagc gcgccgcgcg gcgagaggac gcgcgtcaat
     2581 gtggtgcggt gatggcggga catactaccg agaccgtccg ggacaccatg ccaattgcgc
     2641 gatccgctcg tggaattgcc acatctgccc acttcttcga gatcagtgaa cgaggattcc
     2701 tgatcagtga acgaggattc ctgcgaagag gttgatggcg gtagccagaa tgctggtgcc
     2761 gaagacgtac gacagcaatg tgtgccggag caccatcgag cggatcaccg cactggagac
     2821 gtcggtgtcg gagacctggt aggtcatgcc gaggttgtag ctgaagtaga agaagtcccg
     2881 gtacgccggc ggctggtcgg agttgaaatc gatcccgccc gcggaatccg cgtagtagag
     2941 gtacgcgtag cgggtggcgt acatcagatg cagtgccgcc caggccatga acacgccgag
     3001 cagcgccgtc gcggcatggc cactgtcgga gttgccgagc aggaatacca cgatgccgac
     3061 cagaccgcac agagcgacgg tgacgacgac gatttcctcg aggacggggc gcaggtcctc
     3121 gcgtcgagtg ttgcgtcggg tcgcggcggc gtccatcggc cacagcacga tccagccgac
     3181 caccacgaag aatgtctggg tcgcggcgat acccgcgaga atggccagcg gggtgtcgct
     3241 ggacaatgcg acggccacgc cgaccgccgc gccgacggcg accgagatgg cgagccgtgg
     3301 gactgcggaa agcgacaaac ggatgctcac gctcacaagc cacaacggta gtgcttcgtg
     3361 gctgctcggc acggcgagag cggggccagg gggtgacggt atcccgccca cggccgggcg
     3421 tacgggacgt ccccgcgtcg tggtcgtgtc acgagctcct acgggaccgg aactcctttc
     3481 ttgagaaacc acagaccttc gatatgtcct tttccgtcga agacgacgcg gaaatctccg
     3541 ggctgcttgg ccatgcgcag tggtatgtcg accaccgtcc gatcgccgga cacgacctgc
     3601 cggggcgctc cgtgcgactg gtagcgaccg aaaccgagct ggtcctggta ttccttccag
     3661 gcgttcgaga gaaccacggg atttccttct tgccgaatcg actcgtcgaa acgcgctgag
     3721 acggcgacga agtttccctt cacgacctgg tcgagggttt tgagagcgag ctgtttgtac
     3781 gggggcgggc tggggaccgt caccggtggc cgggccacga ccgcggtgcc ggtggtatcc
     3841 ggcagcgcgt gcgacacggt tccggtgacc agcatcagcg cgctggaagc gacggtgacg
     3901 gcgacgccga aattgccgag cctgttgaac atggatcctc cgactgggtg tccgtggcgg
     3961 gctcacggag tgagcacacg gcctctgacc tggcggttgt gacggcggcg catcacctcg
     4021 aaggggagca ccgtggcccc gcagcaagcg gccactccca cccagatcgg cagcatgaaa
     4081 acgagcgtga atgagctgac gatccagccc gtcgtgaacg tcacgtcggc ccatagggga
     4141 aaaagcgcga gccgggtgga gaacagaggg atcggtcgtc ctgggctgcg cgacgcggcg
     4201 tactgcacat aggtggtgct ggacaatgcc ccgacgatga cggcggccgt gacggtgatg
     4261 atctgcatga gaatctccta acctgagaaa ttctgcatcc agaaaagttc accgaggggc
     4321 ttcgcgtcat gtcacggaac agcgtgactt gaaatcagga accgatcttg ataaatgccg
     4381 cgcccgcatc cctacacggg tgaatggccc accaggtagt cccgcacggc ttcgggcagc
     4441 cccgcgtcga gggcttcggc gaacggagcc cagcggatct gttctgggcc gagtcgcgcc
     4501 tccgccgagg gggcctggct caacagctgg cacaccacgg cgggccgtgc gtcctctcca
     4561 gccgtcccct gcgacctgag gaccgatgtg ccgtcgacca ggtacccggt gagctcgtac
     4621 accaggcgcg ccgcgccggc ctgtgccgac tcggcgggct gcggggcgcc cgacggcagc
     4681 ccccagctgt cctgcgcggc caccatcagc agacgcccgt cgtacacgac gaccgcgtcc
     4741 accccgcccc gggcagcctg ctccagcgtc atggcccaac cgtccctttc ctcctcgcgc
     4801 gaccggccga ccggccccgt ccgagccccc aattcctacc cggggcacat cggttcacgc
     4861 ggcacggcga gctcgtgacc acgctcgcaa acagcggaat cgcccatcgt cacccagctg
     4921 ttccacctgc acaacgggag cgggaatgac cagtgaagcc gtttcccagg gcgatccccg
     4981 tccgctcggg cgccgtgcgg tcacgagccg agtgccacca cggcctcgtc ggtgtacacc
     5041 aggaaggtca ggctctggcg gaagtacaac tcgatctcgt tcgcgtcatg cgcggtgtag
     5101 ccgatcgcga ggtcctcacc gagccgcagt tcgaagtccc caccgcgggt ggacagcaga
     5161 aaggcgccgt cgaccgcggg cgcccagatg ggcggggcgt ccagcatccg gctgagatgc
     5221 tccgtgatcg gatagccgtg gtcggacgtc tcgctcacca gggtgtacgc gtcggcgccc
     5281 agcagcagcg aatacgggcc ggcgactccg gccaggcgca gggtcgtcag cgcgcggctg
     5341 acggcatccg ggtacgcacg tgtctccacg ggcagagcgt gcacaggatg ggaggcacgc
     5401 ctgcgcagcc cgtcgatgcc cgcagcctcg tacccctcga agacggcccg gtcctcggtg
     5461 aacgccatgg cgcgcgccgc gtccttgacc ggttgccagt cggagtcgtt cgagccgcgc
     5521 tccacgtcgt ccaccgcatc gcgaccgact ttgaagggga cgcgcagttc gaccaggggc
     5581 tgtacttggc gcaggagagc agtgacgtcc ggtccgggcc cttcgatgcg ggtgagatgg
     5641 ccggtaccga ccgaggcaag cgcgggaccg tcgggtccgg tgacgtccac gacacgacgg
     5701 ccccccacgt tgcgctggaa ggtacggcgg gcttcctcct cgatctgtgc ccatgcctcg
     5761 ggcgtgatcg gcgccagttc gcggtgcagg ttgttcatac cgttgggcgt ggacacgtga
     5821 tcaggctcct ttgagactgc cgatccccag ggagccctcg gtcgccgacg ccccggtggg
     5881 cgcggcgggt gggtcgtcga ggaaatcggc gctcggtacg tggaacagac agccggtgac
     5941 cgccgtggaa aagtcgagga ttcggtcgtg ggtatggggg tggtcaccga ggaacatgtt
     6001 cctcagcatc tgttcggtca cctcgggagt gcgcgcatag ccgatgaagt aggtaccgaa
     6061 ctccctttgc ccgatgctgc cgaacggcat gttgtcacgg acgatcttcc gttcgtttcc
     6121 gtccgcgtcg gtgatggtgt tcagtgcgac atgcgagtcc gcgggcttga tgtcgtccgg
     6181 catttcgacg ttcgccagct ttgtgcggcc gatgaccttt tcctgttcct gcgtgggcaa
     6241 cgcgttccag gcggcaaggt cgtgcacgta cttctgcacg atcacgtagc tgccgcccgc
     6301 gaaatcgggg tcctcgtcgc cgatgaacac cgcgtcggcc gcgacccggc cctcggggtt
     6361 ctcgctgccg tcgacgaagc cgaggaggtc gcgctcgtcg aagtacttga aaccgtgtac
     6421 ctcgtccacc acggcgaccg ctccgtagag gcgttcgccg atcaactggg ccagttcgaa
     6481 acaaaggtcc atgtggtggg ctcgcagatg gaagagcagg tcacctgggg tggacggggc
     6541 tcggtgccgg gagcccgtca aagcgacgaa cggatgcaga tcgcgtgggc gtggtcctgc
     6601 gaagagccgg tcccaggcgg cggatccgat gccgacgacg caactcagct ccgcgtccgg
     6661 agcgcggaaa ccgaccgatc gtcgcaaccc ggccacatcc tggagcagat cgcgaacagc
     6721 gggctcgccg ccaggtgcca tggtgcacac gaggaacagg gcggctttgg cgggcggggc
     6781 gatcacggac tgcgactcaa ccacggagcc tgcctcctac ttgtcgtggc tatggcgcta
     6841 cgcacggcag gataacgtga ggaaacacaa taaatactag gattgggtca tggtgatgtc
     6901 gaccggtgcg ggtacaccgg cgcctgcctg tgcgaatcgc ccccgacccc tggtggtcga
     6961 ggattggcgc ccccacccat tgcgccttct tcacgaggga atcctggcga tccgcaatag
     7021 gggtcgtaaa agggttgtgg cggacaggac ttcgaggcga agacccgccg gggcgaaccg
     7081 gtctacgcca cggggccgag gttgccttcg agggtcgtcc gagtgatcgg agggcggtga
     7141 cgggggcgag gctgagcggc tccccctccc acacgccgat ggacaggacc tccagctcgg
     7201 gatgggcggc ggcctcgaca ctgctcaggc ctccgatgtt cacccgggcc acggccggtg
     7261 gctggtcccc gcgagagccc cggcgtcctt cgccgagtcc gttcgacacc aggtcggtga
     7321 gggagtcggc gagcaggtcg ccaccgacgt cctgctcatg gtcgcgcatg atgatctgtc
     7381 cgcgtgtccg tgcgggcccg gtgtcaggtc caccgcgacg cggtcgccgc cgccgctgtg
     7441 gccgaagacg atccagccgg gggagccgac cagcccctgc accgcggcgt cggacggggt
     7501 gccgaccgcc tagagctcgc agccgaccgc atcggagacg cgctccgccc gctcgtagtc
     7561 gtcgccccag tcctcccacc gcgcccgggt cacgcggtag agcaccttca gctcgtcggg
     7621 tgtcctcgat ctggagggct cgtccgggct gcgggcgccg ccgcggcagg ggcaggctcg
     7681 acccgtgtcg gccgatcttc cgccacgtag ccgcccggtg cggcgatgct ctccgcgtcg
     7741 gcggttcgca gaagccgcag tacgggctcc caggtcgcga agtcatgtaa tgagggcaac
     7801 ggcggtctcc ggtcgctctg gtgaaccggc ctcgcgcttt cgcgagtacg gctggcgggt
     7861 gctgatggtt gctcaaagag ccgaggagcg ccgccgacct gggacgaagg gacctgtcaa
     7921 gggtcccgct cgtcgccggg agagccgcac tcctcaggtg agtcagcgaa tcgggacctc
     7981 ctcgcggggg tgtgctgaga gcgacagcca ccgcgcggtg gcgcaggccg ttgccgagct
     8041 gcccatggct acaagttgta gcctcccgac acctcgatgt tctgagcggt gacccagcgg
     8101 ctctcctcgg agagcagggc ggcgatcatc atgccgatgt catcgggttc gccgacccgg
     8161 ccgagagcgg tcttcgccgc gagggccggg acgacttcgg gaaaccgctc gaaggcgtcg
     8221 tcggcgatcc gggtgcgcgt cgagcccggc gaaatggaat tgacgcggat gcctcgcgtg
     8281 ctgaactcct tggccatgta gcggctcagc acgtcgaggc cgcccttcat cgacgcgtag
     8341 gccgagtatc cgggctccag gcccgacccc atcgccgagt tgctgctcgt gttgacgatg
     8401 gctccaccgt cttccatcag cggcagcagg cgttgcgtca ggaagtacgg gcccttgagg
     8461 agaacccgca tcagcctgtc gaacatctcc tcggtcgtat cctcgatcag cgacgtgtgt
     8521 gcgaagccgg cgttgttgac caggtagtcg aacgtgtcgc gctgccaggt gtcgcgcaac
     8581 acgtcggcca cggagttgcg gaaggccggg aaggcgccgg tctcaccgac gtccagcggc
     8641 agcgcgacgg cagtgccccc gtccttctcg atcgcggccg ccgtctccaa ccctccctgg
     8701 gagttgttgc cgtaggtcag aatgactccg gttcctcgcc tggcgatctg gatcgccgcg
     8761 ctccgcccga taccggagct ggcgccagtg atgatggcta ccttcatgat ggttctcctg
     8821 gggtgtggtt gaggttccgc cgaggcccgg cgcgggcctc ggcaacacgg gtgtcacgcg
     8881 ggaaatgcca tgaccggcca gggagtcggc cggtgtggcg tcgtctgctc cgctatgcgg
     8941 cagaggttgg ggacaggact cgggcgagcc agtcgttgcg tgtgcggcgg gcctcggccg
     9001 agacacgcgc ctgcgggtac agggcgtcga acccgtggaa gcctcccgcc cagacgtgga
     9061 gttccgcctg gccgccggcc gcccagatac gggtggcgta ggcgacatcc tcgtcgcgga
     9121 agacctcggc cgagccggcg tcgacgtagg tggtcggcag gcccgagagg tcgtcggcca
     9181 ccgcggggga gacatggccg ggcacccgct cgtcccgaag gtgaccgagc accgagcgcc
     9241 agccgaactc gttcatctca cgggtccaga caccgggatc gccggaatac tgccggctcg
     9301 agatggtgac attgcggtgg tcgagcatgg ggcagatgag cacctgggcg gcgatcggca
     9361 gggtgccggc gtcgcgtgcc atcagggtga ccccggcggc gaggccaccg ccggcgctgg
     9421 cgcccgccac gacgatgcgg gcggggtcga tgcccaactc atcggcgtgc tcggcgatcc
     9481 acagcagccc ccggtagcag tcgtcaacca gagtcgtgcc gccgacttcg ggcgccaacc
     9541 ggtagtccac ggagaccacg acggcaccga acaggtcgag ccactccagc gggatgtcga
     9601 tctgtgagaa gcgatctccc atgaccatcc caccgccgtg tatccagaag acgcacggtg
     9661 cccccgtggc gtgcgcggtc ccggcggggc tgaagaccga caggggaatc cgcgcaccgt
     9721 cctcgcccga cacggtgacc tcgcgccggt cgaccgagcg gccttcgaga aggggctcga
     9781 ccggtgtcga cgggagcaga cgcaactgcg cgagcagttc ggggccgagg tgggtcatga
     9841 gcggcatgtc ggcgagcagt gcacccagtt cggggtccaa ggcgggtcgt gcgagagtca
     9901 cggtgactac ctttcgtcgg gtcgtacggg atggtcggag gtggagtgcg cctgcccatc
     9961 ggcgtgcgga agggctgggg atccaacagc ggcctcagaa ggcggccttg ccgtgaaccg
    10021 gtacgtagtc ggtgtactcg gggtccttcg ccagcagccc gtcgaaccgc ttcacgatgg
    10081 cctgagcggc ttcgccggcg aagacccggt ggtggtcctc ctcgctggtc cagccctcgg
    10141 tgacatggac gacgtcgggg ttggacgccg aacggcagac gagatagacg aggcagtgtt
    10201 cactcgtgcc ggggttgccg tcgctcaggc cggtcagcag caggtcggcc agctggtcgc
    10261 ccatgccggg cctggcggtc agggtggcgt tgaatccgtg gttgacgatc atgactacct
    10321 tcttccgcga gggacggatc gtgcaggtgt gctggtcccg gagcagaaga ccctttggct
    10381 ctgcgctgga ctccagtgac tccattcaag cggtctgacc tgcggaaacg gtagaacgat
    10441 gctgtcggct ccttgcacaa tcctcttccg tgcaggagtc ggcgacaggg cgtgtgctgc
    10501 aatgaaggca tgtacctcga agagctccgc accttgctgg ctcgacacgc acggccggag
    10561 tggaccaccg ccatcgacgg cgtcctcatc tcgaaggtcg agcggtccga cccgccggcg
    10621 ccgtcgatga ccggcacggt gctcgcggtg atcgcacagg gcgccaaacg cctcgcgctg
    10681 ggcgaccggg tgtacgagta ccgcgctggg cagtacctcg tcgcgtcggt ggacctgcct
    10741 gtcaccggcc agttcaccga cgccgacccg gagcacccgg cactgggttt cggtctca
//
