LOCUS       NZ_CP041405            10384 bp    DNA     circular CON 07-APR-2024
DEFINITION  Paenibacillus thiaminolyticus strain NRRL B-4156 chromosome.
ACCESSION   NZ_CP041405
VERSION     NZ_CP041405.1
KEYWORDS    RefSeq.
SOURCE      Paenibacillus thiaminolyticus
  ORGANISM  Paenibacillus thiaminolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1  (bases 1 to 6612542)
  AUTHORS   Hehnly,C. and Zhang,L.
  TITLE     Paenibacillus thiaminolyticus NRRL B-4156
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6612542)
  AUTHORS   Hehnly,C. and Zhang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUL-2019) Institute of Personalized Medicine, Penn
            State College of Medicine, 500 University Dr, Hershey, PA 17033, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2018
            Assembly Method :: Canu v. 08/2018; Pilon v. 08/2018
            Assembly Name                     :: PSU_Pthia_1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_007066085.1-RS_2024_04_07
            Annotation Date                   :: 04/07/2024 14:58:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,886
            CDSs (total)                      :: 5,776
            Genes (coding)                    :: 5,673
            CDSs (with protein)               :: 5,673
            Genes (RNA)                       :: 110
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 52 of 103
            Pseudo Genes (incomplete)         :: 68 of 103
            Pseudo Genes (internal stop)      :: 17 of 103
            Pseudo Genes (multiple problems)  :: 28 of 103
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 23:43:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2876918
            Orig. end                         :: 2887302
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP041405.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Schiff/Broach Lab 500
            University Drive Hershey, PA 17033
            Chromosome not circularized.
FEATURES             Location/Qualifiers
     region          1..10384
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="8"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10384
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10384
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5384](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5384)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            639..2375
                     /db_xref="GeneID:76996873"
                     /locus_tag="FLT43_RS12965"
                     /old_locus_tag="FLT43_12965"
     CDS             639..2375
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:76996873"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /locus_tag="FLT43_RS12965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_12965"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_164776389.1"
                     /transl_table=11
                     /translation="MNKPYFYVSITLHLALGLLPLLSVWTMQELVNEVILIAQPGHGFA
                     TALSILSFQMVIILSAYILQFFSQLNDQKLENLLGLVLKKQIFRKIMNLPYSTFEDPSF
                     YDQNQRIINSHFQIVSMVKAIMSLGSTIITVVSLVGYITHIHWGLLVIIFLFSIPILMI
                     HIRFGNKRYALTRFLTPYHRREMYISDLLSQRDSLKEIRLFGLGSHLIEKWSSFYKRSA
                     QEKYSLSKNQGIWELAGSMLLTLTYIVSGILIILLISAKRIEIGAFVAALQSIQNIQSS
                     LTEISASLSNIYEISLYIEDFKSFQQKKELTSSPSQIKCGAINQISVQNLTFTYPNQIE
                     PTLKNIHLSIKKGQKIVIIGENGSGKTSLIKCILGLYDTGENTICVNQIPLHNYDIASY
                     HQRIAALFQDFIEYEFSARENIGFGNLNNPSKPEVIAAAKKTKIHEHISKLPEHYESLL
                     GRHFEGGHELSGGQWQKLALSRALFKNSDVIILDEPTSSMDPESELDIIHELFQGNDDK
                     AVLIITHRLGVAVQADYILVMKEGRIVEEGTHQELLRQQGEYHRLYRAQTKIYNQESGV
                     FV"
     gene            2378..2917
                     /db_xref="GeneID:76996874"
                     /locus_tag="FLT43_RS12970"
                     /old_locus_tag="FLT43_12970"
     CDS             2378..2917
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:76996874"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /locus_tag="FLT43_RS12970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_12970"
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="WP_087444528.1"
                     /transl_table=11
                     /translation="MALVVMFQNVLAFLFVSTFLHKTLNYREHIRIMNAYHLVPARFIV
                     PSFILFAAAELAIGLTLLLSGITLYHVVSIGSLLSIYTFAIGFNLMRGHRHIDCGCGGI
                     LENEQLSWGLVLRNLLLGFSVAGIWKSQSLYIVEIALIHKLELVMLSLILFSLLCLFKE
                     LFNIRKKLVRLMKMFI"
     gene            2942..3520
                     /db_xref="GeneID:76996875"
                     /locus_tag="FLT43_RS12975"
                     /old_locus_tag="FLT43_12975"
     CDS             2942..3520
                     /GO_function="GO:0016209 - antioxidant activity [Evidence
                     IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:76996875"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /locus_tag="FLT43_RS12975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_12975"
                     /product="redoxin domain-containing protein"
                     /protein_id="WP_087444527.1"
                     /transl_table=11
                     /translation="MTDFILIITLVLVLIQLLLIFMLARLIGQFLAKIKSVGGISLTEL
                     QIGEQVPLFREWGSQGRKVILRELLSHRKVLLLFISTTCQTCKSILPDLQRVVENWDIH
                     IVVINTDKQSNDEEIGAMLPSGIDYIRSVDIMDAYGVSKVPYAFLVNQNGILESHHSLM
                     SANMLWNMLENETARSNDQVPSPLSVMNE"
     misc_feature    complement(2943..2957)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
     gene            complement(4001..4903)
                     /db_xref="GeneID:76996876"
                     /gene="thpD"
                     /locus_tag="FLT43_RS12985"
                     /old_locus_tag="FLT43_12985"
     CDS             complement(4001..4903)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /db_xref="GeneID:76996876"
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679980.1"
                     /locus_tag="FLT43_RS12985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_12985"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_280525910.1"
                     /sec_met_domain="PhyH (E-value: 3.7e-37, bitscore: 127.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRGQSQAQEAARTPDMYPSRVNTETLLLKRPDPVVYNHTIGQTEI
                     TADQLASYEADGYLFLDSFFSEEEVQVWRRELLRLFQDHKDSAKPEVIREPGSDEIRSI
                     FAVHREGLFRQLANHPRILPIVQQILGSQVYVHQSRINFKQGFTGKEFYWHSDFETWHV
                     EDGMPRMRALSCSIALEENNANNGPLMVIPGSHRMFVSCIGATPDDHYKSSLRRQEYGI
                     PDQESLIYLANQGGIVAPTGKAGSILLFDCNVMHGSNSNITPFPRSNVFIVYNSVENPL
                     EPPYSGKPPRPDFIAARSS"
     gene            complement(5001..5384)
                     /db_xref="GeneID:76996877"
                     /locus_tag="FLT43_RS12990"
                     /old_locus_tag="FLT43_12990"
     CDS             complement(5001..5384)
                     /codon_start=1
                     /db_xref="GeneID:76996877"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679979.1"
                     /locus_tag="FLT43_RS12990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_12990"
                     /product="ectoine synthase"
                     /protein_id="WP_087444524.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.2e-51, bitscore:
                     170.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKQLSDILGTEHDVKAETWNSRRLLLKKDGVGFSLHDTLIKAG
                     TETEIWYKHHVEAVYCIEGEGEIEVVESGVVYPIQPGTLYVLDGHEKHLLRGKSQMRML
                     CVFNPPCTGNEVHDAEGTYPLEV"
     gene            complement(5499..6782)
                     /db_xref="GeneID:76996878"
                     /gene="ectB"
                     /locus_tag="FLT43_RS12995"
                     /old_locus_tag="FLT43_12995"
     CDS             complement(5499..6782)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (31-366). E-value: 1.2e-78.
                     Score: 256.5. Matches aSDomain:
                     nrpspksdomains_FLT43_RS12995_Aminotran_3.1"
                     /codon_start=1
                     /db_xref="GeneID:76996878"
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679978.1"
                     /locus_tag="FLT43_RS12995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_12995"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_087444523.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-75, bitscore:
                     253.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLDGRTTSLQVFESLESEVRSYCRSFPTVFTKAQGYKLWDADN
                     REYIDFFAGAGALNYGHNNRTMKRKLLDYIQQDGVTHSLDMATKAKEQFLKKFHRVILE
                     PRGLAYKVMFPGPTGANTVESALKLARKVTGRSTVFSFTNAFHGMTLGALSVTGNKFKR
                     QGAGIPLHHTVTMPYDGYLGSDIDTLDYMECYMNDSGSGVSRPAAIIVETIQGEGGINE
                     ASNEWLQRLERVCRSHEIVLIVDDVQMGCGRTGTFFSFERAGITPDIVCLSKSIGGYGL
                     PMALTLIRPDLDIWEPGEHNGTFRGNNLAFITAAEALDYWNDERFTQAIMAKGKMIRSC
                     LEELVAQYPELQGTARGRGLMQGIAFGIPGMAERLCARAFAKGLIMETSGTDSEVAKIM
                     PPLTIDDTGLLAGLDILRTCMKEMAEEL"
     aSDomain        complement(5685..6689)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FLT43_RS12995_Aminotran_3.1"
                     /evalue="1.20E-78"
                     /label="FLT43_RS12995_Aminotran_3.1"
                     /locus_tag="FLT43_RS12995"
                     /protein_end="366"
                     /protein_start="31"
                     /score="256.5"
                     /tool="antismash"
                     /translation="FTKAQGYKLWDADNREYIDFFAGAGALNYGHNNRTMKRKLLDYIQ
                     QDGVTHSLDMATKAKEQFLKKFHRVILEPRGLAYKVMFPGPTGANTVESALKLARKVTG
                     RSTVFSFTNAFHGMTLGALSVTGNKFKRQGAGIPLHHTVTMPYDGYLGSDIDTLDYMEC
                     YMNDSGSGVSRPAAIIVETIQGEGGINEASNEWLQRLERVCRSHEIVLIVDDVQMGCGR
                     TGTFFSFERAGITPDIVCLSKSIGGYGLPMALTLIRPDLDIWEPGEHNGTFRGNNLAFI
                     TAAEALDYWNDERFTQAIMAKGKMIRSCLEELVAQYPELQGTARGRGLMQGIAF"
     gene            complement(6779..7324)
                     /db_xref="GeneID:76996879"
                     /gene="ectA"
                     /locus_tag="FLT43_RS13000"
                     /old_locus_tag="FLT43_13000"
     CDS             complement(6779..7324)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:76996879"
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679977.1"
                     /locus_tag="FLT43_RS13000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_13000"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_244194346.1"
                     /transl_table=11
                     /translation="MSMDVNVTQSLPCTIRNARNGDGTDIWKLVTESGTLDVNSAYCYI
                     MLCEYFADTCIVAEHNRNIIGFVSALRPPEMPDTVFVWQIAVRAGYRGRGIAEALLRTL
                     YEAACRDRVRYMETTIAPSNKASQRLFAKMAKTWKSALVTKEGFSSQLFPEGAHETEKL
                     IRIGPLRHNIHDRMETVT"
     gene            complement(7321..8187)
                     /db_xref="GeneID:76996880"
                     /gene="ehuA"
                     /locus_tag="FLT43_RS13005"
                     /old_locus_tag="FLT43_13005"
     CDS             complement(7321..8187)
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:76996880"
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679976.1"
                     /locus_tag="FLT43_RS13005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_13005"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_087444521.1"
                     /transl_table=11
                     /translation="MESVQCRPLASQAAHDPIVMYRNICKSYGSVRVLQDINLSIYPGE
                     KVAVIGPSGSGKTTLARILMTLERPTSGTIQVDGEWLWHQEVKGKLVAAKEKHLHNVRS
                     KIGMVFQHFHLFPHMTILRNVTEAPVHVLGWNKQEAEARAMEMLAKVGLEDKVKEYPAR
                     LSGGQKQRVAIARAVVMRPKVMLFDEATSALDPELVGEVLTVIKDLAAEGDMAMMLITH
                     EMDFAREIADRILFTDGGSIVEQGTPEHIFENPQSPRLQAFLSRFRQTWYMGGVQQASP
                     SAEEGGR"
     gene            complement(8204..8854)
                     /db_xref="GeneID:76996881"
                     /gene="ehuD"
                     /locus_tag="FLT43_RS13010"
                     /old_locus_tag="FLT43_13010"
     CDS             complement(8204..8854)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:76996881"
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679975.1"
                     /locus_tag="FLT43_RS13010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_13010"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_087444520.1"
                     /transl_table=11
                     /translation="MWSWDYALEILPQLLQVLPITIGATLLGFAAACMLGLPLALARRS
                     RYRAVSVLANAWMEFIRSTPLLVQLFVLFYALPLYGISFSPFVTGVIGLGLHYSAYLSE
                     VFRSGIEAVPAGQWEAAKALNFGRRHSWTNIILPQAIPPIIPVMGNYLITMFKETPVLS
                     AITLVELLQTAKAIGSGSFRYLEAFTMVGLLFLLLSYPSSLLVSRLEKRFQSK"
     gene            complement(8858..9520)
                     /db_xref="GeneID:76996882"
                     /gene="ehuC"
                     /locus_tag="FLT43_RS13015"
                     /old_locus_tag="FLT43_13015"
     CDS             complement(8858..9520)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:76996882"
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679974.1"
                     /locus_tag="FLT43_RS13015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FLT43_13015"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_087444519.1"
                     /transl_table=11
                     /translation="MKSASAILEYVPALLQGAWVTLQIVCFSMMLAFFCAFIAGLARLS
                     KYRIIRFVSAVYVEVFRGTSLLVQLFWLYFALPMLLDIRMSAMAAAVLALGLNYGAYGS
                     EVVRGSILSVPKGQYEAAVALNMSPWLRMRRIVLPQAMALMLPSFGNLLIELLKGTSLV
                     YFITLMDLTYQGMVLRSFDNSSTPQIFALLLLLYFIMAYSLTLGIRWVERRAVKGRV"
ORIGIN
        1 tcacatagac aaagtacgga tattccgtaa tccggtagga ttcaaatatg ttctcgccgg
       61 cttgcaaaaa ttggattcca ttactatggc tatacagttc tttgtagccg ccaattccgc
      121 tttcttttag caaaactgca gcatttagag ccgttccccg ctccatactt gcttgaatcc
      181 atctctctaa tgacgggatg caataagggc agtgtgaatc gataaatatg agcaggattc
      241 cattcttcgc tatggcatgg cttaaatcaa aacgggtatc accggcaccc ggcaagaaaa
      301 atgagggagc ttgctggtgc agtaaggggt ataggcggcg gctctcccct tctccctcca
      361 tgctgttcgt ccgctgaagc atagaggaca agaatctgta gacaacgacc gttgcgaata
      421 taacacacgc tagcaaagcg aattgaaaat agatagccac attcaacctc cgccataaaa
      481 atctcgtcct tgcgcatctg ccatattatg tatatactca gtttatatca tacctgtctt
      541 ttactatgat ggcaaatatt gatgaaaggc gaggaataat gtattctttt tcaaatattt
      601 ggcgcggttc cgggtttgcc cttctttata tttggagatt gaacaaaccc tatttttacg
      661 tgtccattac gcttcatctc gcgctaggct tattgcctct gctgagcgta tggacgatgc
      721 aagaactcgt gaacgaggtt attttaatcg ctcaacccgg acatggattt gccacagcgt
      781 tatcgatttt aagcttccaa atggtcatca ttctgtctgc ctatattctt caatttttct
      841 ctcaattaaa tgatcaaaaa cttgaaaatc tgctcggact ggtgctcaaa aaacaaatat
      901 tccggaaaat tatgaatctg ccatactcca cctttgagga cccgtctttc tatgatcaga
      961 accagcgaat tatcaatagc cacttccaaa tcgtgtcgat ggttaaggcc atcatgtctt
     1021 taggaagcac cattatcacg gttgtgtcgt tggtgggcta cattacccat atccattggg
     1081 gattgcttgt cattatcttt ctattttcga ttccgatcct gatgattcat atccgttttg
     1141 gcaataaaag atatgcgctc actcgatttc tgactcccta ccacagaaga gaaatgtaca
     1201 tatccgatct cctaagccaa cgagattcct tgaaagaaat tcgcctcttt ggactgggct
     1261 cgcatctgat cgaaaaatgg tcttcattct ataagcggag cgcgcaagaa aaatacagtt
     1321 tatccaaaaa tcaaggcata tgggagcttg ccggcagcat gctgctcacg ttgacctata
     1381 ttgtgtccgg tattctcatt atcctgctga tctcggccaa gaggatagaa ataggggcgt
     1441 tcgtggcggc cctgcaatcg attcaaaata ttcagtccag cttgaccgaa atttccgctt
     1501 cactatccaa tatctatgaa atcagtttgt atatcgagga tttcaaatct tttcaacaaa
     1561 aaaaggagct gacaagttca ccttctcaaa tcaaatgcgg cgccatcaac cagatcagcg
     1621 tacaaaatct cacgtttact tatccaaatc aaatagaacc gaccctcaaa aatattcatc
     1681 tgtctattaa aaaaggacaa aagatagtca tcatcggaga aaacggctcc ggcaagacct
     1741 cgctgatcaa atgcatacta ggtctgtatg atacgggaga aaatacgata tgcgtcaacc
     1801 aaattcctct acacaactat gatatcgcat cctaccatca acggattgcc gctttatttc
     1861 aggatttcat tgaatatgaa ttcagcgccc gggagaatat cggctttggc aatctgaaca
     1921 acccaagtaa gcccgaagtg attgctgccg ccaaaaaaac gaaaattcat gagcacatat
     1981 ccaagctgcc tgagcattat gagtcactgc taggacgtca ttttgaagga gggcatgaat
     2041 tatccggggg gcaatggcaa aagctggcct tatccagagc tctattcaaa aatagcgatg
     2101 taatcattct tgacgagcct acctcatcca tggacccgga atcggaactc gatattattc
     2161 atgaattatt tcaagggaac gatgacaaag cggtgcttat cattacgcat cgtcttggcg
     2221 tcgccgtgca ggcggactat attttggtga tgaaggaagg ccgcatcgtc gaggaaggca
     2281 ctcatcagga gcttttgcgc caacagggcg aataccatcg tttatatcgc gcgcaaacca
     2341 aaatttataa tcaggaaagc ggggttttcg tctaaacatg gctctcgttg ttatgttcca
     2401 aaatgtgctt gcctttttgt ttgtttccac ctttttacat aagacactga actaccgcga
     2461 acatattcgc atcatgaatg cttatcacct tgtgccagcc cgatttatcg ttccgtcttt
     2521 tatattattt gccgcggccg agcttgcgat aggcctcacg cttctcttgt ccgggatcac
     2581 gctgtatcat gtggtgtcca taggttcact gctaagtatt tatacctttg caatcggttt
     2641 caatttgatg cgagggcatc ggcatataga ttgcggatgt ggcgggattc ttgaaaatga
     2701 acagctgagc tggggattag tgcttcgtaa tcttcttctc ggtttttcgg ttgcaggtat
     2761 atggaaatcc caatcattgt acatagtcga gattgcgctt atccataaac ttgagctggt
     2821 catgctctcg ctcatccttt tttcgttgct ctgtctgttc aaggaattat tcaacatccg
     2881 gaaaaaactt gttcgcctca tgaagatgtt tatctaaaaa ccaacgagga aggagattcg
     2941 aatgaccgat tttatcctta tcatcacttt agtgctggtg ctcatccagt tgctgcttat
     3001 attcatgctg gcaaggctca ttggccaatt tcttgcgaaa ataaaaagcg tgggcgggat
     3061 ttcgttaacc gagcttcaaa tcggagagca agttccgctt tttcgggagt ggggctcaca
     3121 ggggcgcaaa gtgattctaa gggaattgtt gagccacaga aaagtgctgc ttctctttat
     3181 cagtacgaca tgccaaacct gcaaaagcat attgcccgac ttacaaagag tggttgaaaa
     3241 ttgggatata catatcgtcg ttatcaatac ggataagcaa tccaatgacg aagagatcgg
     3301 ggcgatgctc ccttccggca tcgattatat tcgttctgtg gacattatgg acgcttatgg
     3361 ggtaagcaaa gttccctatg cttttctagt gaaccaaaac ggaatcctag aatcccatca
     3421 ttcgttaatg agtgcgaaca tgctgtggaa tatgctagag aacgaaacgg cacgttccaa
     3481 tgaccaggtc ccaagcccct tatcggttat gaatgagtag ggttagcgtg gtaagaaaaa
     3541 cggcaacaga aagaagcgat tgtccatctt ggcagaggcc aaatcgcttc cctggttcat
     3601 ccattttatt ctggcgaatc tggcgaaaat aggcttgttc ctagcagccg catcttactt
     3661 ttgtcggcct tgtacactct tctcccgtat cacgatcaac atagtactcc caggaggaca
     3721 ctttttgcat aatgcacagg gcgtcggagc ggcaggagac aactctgcgc gttaagatgc
     3781 agcgtgcggc agcaggctgg aatcctgtca acttgctcaa gaaatttctc actctcatta
     3841 cctccattac taatatttta tttcatacac attatatacc ttttatatta taaaatccat
     3901 atttggtttt attatcaagc atgaggcacc caatgctgga cgtaaggtaa aagaagaccc
     3961 atccgcgatg ggtcttctct cttttgatca ctgtcttctg ctaggatgaa cgggccgcaa
     4021 taaagtcagg tcttggcggc ttgcctgagt acggcggttc aagcggattc tcgacactgt
     4081 tatagacgat gaatacattg ctgcgcggga acggcgtaat attgctgttc gagccatgca
     4141 tcacattgca gtcgaacaat agaatggagc cggccttgcc cgtaggcgcc acaatgccgc
     4201 cttgattggc cagatagatg agactctcct gatcgggaat gccgtattcc tggcggcgca
     4261 gcgatgactt ataatgatcg tccggcgtgg ccccgataca agagacgaac atgcggtgcg
     4321 atccggggat gaccattaac ggaccgttgt tcgcattgtt ctcttccagc gcaatggagc
     4381 agctcaatgc cctcatgcgc ggcatgccgt cttccacatg ccatgtctcg aagtcggaat
     4441 gccagtagaa ctctttgcct gtaaagcctt gcttgaaatt aatgcgcgat tgatgcacat
     4501 agacttggct gccgagaatc tgctgcacga tcggcaggat tctcgggtgg ttcgccagct
     4561 gccggaatag gccctcccga tgcacggcaa aaatcgagcg tatctcgtcg ctgcccggct
     4621 cccgaatcac ttccggcttc gcgctgtcct tatgatcctg gaacaagcgg agcagctccc
     4681 gcctccatac ctgcacttct tcctcgctga agaacgaatc gaggaacaag tatccgtccg
     4741 cttcgtaaga agcaagctga tcggctgtta tctccgtctg cccgatcgta tggttgtaga
     4801 caaccggatc agggcgcttt aacaataagg tctccgtatt cactctggac ggatacatat
     4861 ccggcgttct tgcagcctcc tgcgcttggc tttgtcctct caatatgatc atgctccttc
     4921 cgtgaaaaag tatggcggtt cgcgttcccg ctttttacgt cagtcgtcat acccttgtct
     4981 tcgtccgcct gcacccgcgc ttagacttct aacggatatg tgccttccgc atcatgcacc
     5041 tcattgcccg tgcaaggcgg gttgaatacg cacagcatgc gcatctggct tttccctctc
     5101 agcagatgct tctcatgacc atcgagcaca tataacgtcc ccggttgaat cggatagaca
     5161 actccgctct cgacgacctc gatttcccct tcaccttcga tgcagtagac tgcctccaca
     5221 tgatgtttgt accagatttc ggtctccgtg cctgccttga tcagcgtatc atgcaaggaa
     5281 aaaccgactc cgtccttctt cagcagcaat ctgcggctgt tccatgtctc tgccttcaca
     5341 tcatgttccg tgccaagaat atcagacaat tgcttgacga tcatcgtaga ttcctcctat
     5401 ttgttcgtgt tctccttaat ttcgcgttcc ccacaatatc gtgttctcaa caatatcgtg
     5461 ttctccaaca tttcaccgtg tgccgatatg acggcatgct acagctcttc cgccatttcc
     5521 ttcatgcatg ttcgcaaaat atccaagcct gcaagcagac ccgtgtcatc gatcgtgagc
     5581 ggcggcataa tcttcgccac ttcgctgtct gtacccgatg tctccatgat gagccccttc
     5641 gcgaaggctc tggcgcagag ccgctccgcc atgcccggga tgccgaaggc aatcccctgc
     5701 atcaatcctc ggcctctcgc cgttccttgt agttcaggat attgtgcgac tagctcttcc
     5761 aagcaagagc gaatcatctt tcctttggcc ataattgctt gcgtaaagcg ttcatcattc
     5821 caataatcga gtgcctcagc cgcggtgatg aatgccaaat tattgcccct gaacgtgccg
     5881 ttgtgctcgc caggctccca aatatccaaa tcggggcgga ttaacgtaag cgccatcggc
     5941 agtccatatc cgccgatcga tttggacaga cagacgatgt ccggcgtaat gcccgcccgc
     6001 tcgaagctga agaaggtccc tgtgcggccg catcccatct gcacatcgtc cacgatgagc
     6061 acaatctcgt ggctgcggca cacccgctcc agccgctgca gccattcatt gctcgcctcg
     6121 ttaatcccgc cttcaccctg aatggtctcg acgatgatgg cggccggccg cgacaccccg
     6181 ctgccgctgt cattcatata gcattccata taatcgagcg tatcgatatc cgatcccaga
     6241 tacccgtcat acggcatcgt gaccgtgtga tgaagcggta tgcccgcccc ttgccgcttg
     6301 aacttgttgc ccgtgacgga taaggcgccc agcgtcatgc cgtgaaaggc gttcgtgaag
     6361 ctgaagacgg ttgatctccc cgttaccttg cgcgccagct tcaaggcgct ctccaccgtg
     6421 ttcgcgcctg tcggcccggg gaacatcacc ttgtaagcca atcctcgcgg ctccaagatg
     6481 acccgatgga atttcttcag aaattgttcc ttcgccttcg tggccatgtc caagctatgc
     6541 gtgacgccgt cttgctgaat atagtccagc agcttccgct tcatcgtccg attgttatgc
     6601 ccgtagttga gtgcgccggc tccggcgaag aaatcgatat actcccgatt gtccgcatcc
     6661 cacagcttat agccttgcgc cttcgtaaat acggtcggga acgacctgca gtagcttcga
     6721 acctcggact ccaacgattc gaatacttgc aacgatgttg ttcttccatc caataccttc
     6781 atgtcaccgt ctccattcta tcgtgtatat tgtggcgcaa tggccctatg cggatcagct
     6841 tctcggtctc atgcgctcct tccggaaata gctgagagga gaacccctcc ttcgtcacga
     6901 gcgcgctctt ccatgtcttc gccatcttgg cgaacaggcg ctgggatgcc ttgttgcttg
     6961 gcgcgatcgt cgtctccatg tatcgcaccc gatcccggca tgccgcctcg tacaatgtgc
     7021 gcagcagcgc ttcggcgatc cctcggcctc gatagcccgc acgcaccgcg atttgccaga
     7081 cgaacaccgt gtccggcatc tccggcggcc gaagcgcgga tacgaagccg ataatattcc
     7141 gattgtgctc agccacgatg caggtgtcgg cgaaatattc gcacagcatg atataacaat
     7201 aggccgagtt gacatcgagc gtgccgctct ctgtcaccag cttccatatg tccgttccat
     7261 cgccattccg cgcgttgcgg atcgtgcagg gcaaggattg cgtgacgttg acatccatgc
     7321 tcatcgtccc ccctcttctg cagatggtga agcttgctga actcctccca tataccacgt
     7381 ctgcctgaac cggctcaaaa aggcttgcag gcgcgggctt tgcgggttct cgaatatgtg
     7441 ctccggcgtt ccctgttcga cgatggagcc gccatctgta aacaggatcc gatcggcaat
     7501 ctccctggca aaatccattt catgcgtgat gagcatcatc gccatgtcgc cttcggctgc
     7561 caagtccttg atgaccgtaa gcacctcgcc gaccagctcc ggatcgagcg cggaggtcgc
     7621 ttcgtcgaag agcatcacct tcggacgcat gactacggct ctggcaatgg ccacgcgctg
     7681 cttctgcccg ccggacaagc gtgcagggta ttccttcacc ttgtcttcca gtccaacctt
     7741 agccagcatc tccatagcgc gcgcttcggc ttcctgcttg ttccatccca acacgtgcac
     7801 cggcgcttcc gtcacattgc gcaaaatggt catatgcgga aaaaggtgaa agtgctgaaa
     7861 gaccatgccg atcttgctgc gtacgttgtg cagatgtttt tccttcgccg ctacaagctt
     7921 gcccttcact tcttggtgcc atagccactc cccgtcgact tgaatcgttc ccgaggtggg
     7981 ccgctccaat gtcatgagaa tgcgggccaa cgtcgtcttg ccggatccgc tcgggccgat
     8041 gacggcaacc ttttcccccg gataaatgga tagattgata tcttgcagca ccctcacaga
     8101 accgtacgac ttgcatatat tccgatacat gacgatcgga tcgtgtgccg cttgcgaggc
     8161 cagcggcctg cattggacgc tctccatctt cgctctcccc cccttatttg gactgaaatc
     8221 tcttctccaa tctgctgaca agcagagagg aaggataact aagcagcaga aataataacc
     8281 cgaccatcgt aaaggcttcc agatagcgga atgagcctga acctatcgct ttggccgttt
     8341 gcagcaattc gacaagcgtg atcgccgata agacgggagt ttccttgaac atcgtaatca
     8401 aatagttgcc catgacggga atgattggcg gtatcgcctg gggaaggatg atgttcgtcc
     8461 atgaatgccg cctgccgaag ttaagcgcct tagccgcttc ccattgaccg gcgggcaccg
     8521 cctcaatccc cgatcgaaat acttcggata gataagcgct ataatgcaag cccaatccga
     8581 tgactccggt gacaaacgga gaaaatgaga taccgtaaag cggtaatgca taaaagagta
     8641 cgaacaactg aacgagcagc ggagtgctgc ggatgaattc catccacgca ttcgcaagca
     8701 cagatacggc tcgatatcgg gatcggcgag caagcgccaa tggcaaccca agcatgcatg
     8761 ccgccgcaaa tccgagcagt gtcgctccga tcgtaatcgg gagcacctgc agcagctgag
     8821 ggagaatctc caatgcgtag tcccaactcc acatacgcta caccctcccc ttcacggccc
     8881 tgcgctccac ccatcgaatg ccgagcgtca aggagtaggc catgatgaaa taaagcagga
     8941 gcagtaaggc aaaaatctgc ggcgtactgg agttgtcaaa ggagcgtaat accatgcctt
     9001 ggtaggtcaa atccatcaag gtgatgaagt acacaagcga tgttcccttc agcagctcga
     9061 tgagcagatt gccgaacgaa ggcagcatga gcgccatcgc ttgcggcaat acgattcttc
     9121 gcatccgcag ccacggactc atattgagcg caacagccgc ttcgtactgc cccttgggca
     9181 cggacaggat cgacccccgc accacttcag agccataagc gccgtagttc aagccaagcg
     9241 cgagcacggc tgcggccatc gccgacatgc ggatgtcgag cagcatgggc agcgcaaaat
     9301 ataaccagaa caactgaacc agcaaagaag tgcctcgaaa gacctctacg taaaccgcgc
     9361 tgacaaagcg gatgatgcgg tacttggaca gacgagccaa tcccgctata aaagcgcaga
     9421 agaaagcgag catcatggag aagcatacaa tttgcagcgt gacccacgct ccttgcagca
     9481 gcgcaggcac atattcaagt attgcggaag cgctcttcat ggcttcactc ttgcgacagc
     9541 cgttcagccg tcatatctcc aggcagctcc tgctccgtga acccgaacgg cctcaatagt
     9601 tcaagcagct cgcccgattg cttcaacgct tccagctctt gattgaatgc ttcccggaag
     9661 gcggtatctt ccttgcggaa cgccgccgct ccataccctc ttacgcttgt gccgtctatc
     9721 tccggctgct ggaagtccat gactcgctcc aattgatcgt ccttcgtcga gtcaagcatc
     9781 gcctgcaagg atggtcccgt catcgtcacc gcatccacgc gccccgcctg caaagccgag
     9841 atagccgacg gttgatccgg caccgttacg atttgttcct tcgcgacacc tgatttcgct
     9901 aaatattcaa gctcgatggc acccaccatg acggcgactg tgacatcctt gttgccagca
     9961 atatcctcgt agctgcgcag atgggacgga ttccccttct tgaccgcgag cgcctcgcca
    10021 atgctgtatt cggggttggc gaaggctact tccttgctcc gctccggatt gatgaacatt
    10081 cctgccgtga tgatgtcgaa gcgcttcgcc ttcaatcccg gaatgagaga accgaattcc
    10141 gtcaggacgc cgttcatctc ctcgatgccc atccgcttca acacttcccg ggctacctcc
    10201 acggcctcgc ccgtcagttg gccatccggc gtggcataag catacggctt ttcattcgcg
    10261 aagcccacgg taacgtatcc ttcccgcctc gctttgtcga gcgccgtctc ctggggcgat
    10321 gtcccgccgc cggcttcggt ctggctgccg ccgctcttcg gcgcacagct taccagcata
    10381 atgg
//
