LOCUS       NZ_VFNO01000020         6867 bp    DNA     linear   CON 16-AUG-2024
DEFINITION  Brevibacillus sp. AG162 Ga0222362_120, whole genome shotgun
            sequence.
ACCESSION   NZ_VFNO01000020
VERSION     NZ_VFNO01000020.1
KEYWORDS    WGS; RefSeq.
SOURCE      Brevibacillus sp. AG162
  ORGANISM  Brevibacillus sp. AG162
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Brevibacillus.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Venturi,V.
  TITLE     Genome sequencing of rice bacterial endophytes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Venturi,V., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 236.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006715575.1-RS_2024_08_16
            Annotation Date                   :: 08/16/2024 02:19:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,054
            CDSs (total)                      :: 5,923
            Genes (coding)                    :: 5,868
            CDSs (with protein)               :: 5,868
            Genes (RNA)                       :: 131
            rRNAs                             :: 1, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 6, 5 (16S, 23S)
            tRNAs                             :: 111
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 25 of 55
            Pseudo Genes (incomplete)         :: 38 of 55
            Pseudo Genes (internal stop)      :: 3 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:50:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6867
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VFNO01000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..6865)
                     /NRPS_PKS="Domain: PCP (130-198). E-value: 4.4e-24. Score:
                     76.5. Matches aSDomain: nrpspksdomains_FB479_RS30220_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (213-518). E-value:
                     6.7e-161. Score: 528.7. Matches aSDomain:
                     nrpspksdomains_FB479_RS30220_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (524-678). E-value: 5.8e-46.
                     Score: 148.1. Matches aSDomain:
                     nrpspksdomains_FB479_RS30220_TIGR01720.1"
                     /NRPS_PKS="Domain: Condensation_DCL (686-986). E-value:
                     3.4e-117. Score: 382.8. Matches aSDomain:
                     nrpspksdomains_FB479_RS30220_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1148-1541). E-value:
                     3.6e-109. Score: 357.0. Matches aSDomain:
                     nrpspksdomains_FB479_RS30220_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (1651-1717). E-value: 6.4e-27.
                     Score: 85.6. Matches aSDomain:
                     nrpspksdomains_FB479_RS30220_PCP.2"
                     /NRPS_PKS="Domain: Condensation_LCL (1734-2035). E-value:
                     1.2e-120. Score: 393.9. Matches aSDomain:
                     nrpspksdomains_FB479_RS30220_Condensation_LCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039961527.1"
                     /locus_tag="FB479_RS30220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_142067649.1"
                     /sec_met_domain="PP-binding (E-value: 1.8e-19, bitscore:
                     56.4, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.4e-119, bitscore:
                     384.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.6e-84, bitscore:
                     269.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYLPDGLIEFIGRIDNQVKIRGFRIELSEVEFVLAKHHALAASVV
                     IVREDEPGKKQLVAYAVKQAEQEVDIAELRQHFKAHVPDYMVPSAFVMLDELPLTPNGK
                     VDRRALPKPDYANTSDGDYVAPHTFIERKLSEIWQDVLRIEEISIHSNFFELGGDSILS
                     IQIVSRANQAGIRLTPKQIFENQTIAELAALVSTTGNGENAIQLAEQGLVTGNVLLTPI
                     QKWFFAANQPSIHHWNQSFLLTVEEPVDTNALERAITELLAHHDALRMRYTNNDGLWSQ
                     HIEGLGEPVPFHLVDLADLSSEEQFARLEEIAGEMQASLNITEGPLFRAVYFHLGKERA
                     GRLLLVVHHLVVDGVSWRILLDDLQLAYEQAVNGQTVQFPSKTTSFKAWAEQLHAYAKS
                     DSLEKEKAFWANQAYEVDPLPVDRTFEPAQNTEASTKQITLTLTAEETRNLLHETLPSY
                     RLQINDVLLASLAKALNRWTGKETMVVTLEGHGREEIIEGADLSRTVGWFTSMYPIQIS
                     TEQEKPWGHTLKAVKEQLRQIPNKGVGYGILRYLCDDLELQHRLNAKPKPQISFNYLGQ
                     FDQTVAVSSTFGIASECSGANLAPDSIRENLLDIISAVTGDQLHVTWLYNENIHDEQTI
                     AAVADDYMEALREIMSYTQAAEEIGYTPSDFPLANLNQQSIDKYIASDRQIESVYALTP
                     LQEGMLFHSLYEQEGGDYVVQFLMTVRDISLEVFEQAWQKIVDRHSILRTSFIWEGLEQ
                     PHQIVRKQVKVSLEKIDLRHVDVDKQEAEIQSYMEKDRVRNFPFNKAPLMRWTLFCTND
                     NTYRFLWSFHHILLDGWSMPLVLQDWQASYQAIDKGEEEKLEAVQPFSRYIAWLNKQDK
                     QVAERYWREQLKGFYEPTPLAMGKAGSHAIQPKAYRDRTVSLSKEVTAHLQAFARKQQL
                     TINTLVQGAWALILRGYSGSDDVVFGTTVSGRPADLPGVENMVGLFINTLPVRVMFDPS
                     QTIQEWLQHLQKTQLDIRQYEYTPLVDVQGWSAVPHGQSLFDSILVFENYLNSTSEDSD
                     SGVSLSEVQAVEQTNYPLTLVAAPGDELALKLIYEQGRFDTEVIGKVLEQMSQVLHALT
                     AQAEQPLRTLSFITDEERHKLLVEWNATETEYTRDAVMHQLFEAQVAATPDAEALIVGE
                     ERLTYAELNRRANQLAHYLRVQGIGPEVLVAVLMERTTEMIVAMMGILKAGGAYVPIDP
                     AYPQERIGYTLEDSQAAILLTQGSLLPMLPEHTAQVICLDRDWESIAEQSEENLLNLVE
                     PTNLSYVIYTSGSTGLPKGVAIQHSSVIAFIAWAKTVFSAEEMSGVLASTSICFDLSVY
                     EIFVTLSFGGKVILADNALHVPSLPAANEVTLINTVPSAAKELVRMNAIPSTVRVVNLA
                     GEPLPNTLAQSLYALEHVEKVFNLYGPSEDTTYSTYVQVTKGATSEPTIGRPLSNTQAY
                     ILDANLQPVPLGLPGELYLGGEGLAREYLNRPELTAERFLSNPFHADPYARMYSTGDLV
                     RYLPDGQIEYLGRIDHQVKIRGYRIELGELEAVLRTHPAVKEAVVTAREDQLGDKRLAA
                     YVTTKEENREEATDLAVWAKAKLPEFMVPSVFVWLEAMPLTPNGKIDRKQLPEPEWGQM
                     VSTQEYVAPRNQTEELVAAIWSHVLGIEKVGIHDNFFELGGHSLLATRVISRLRDMFAG
                     EVPIRTLFENPTVAELSEALGSFLQETGASIEPVSREGHLPLSFAQQRLWFLDRLMPDS
                     ALYNIPLAIRLHGDLNMEAWNKSLQVLIQRHESLRTTFDHVDGQAVQIIHPYQEQQLRV
                     IDLRELPADECEAETQRLAGREAATPFNLSEGPLFRTTLIRISEQETVFLLNMHHIISD
                     GWSMGVFIREWFTTYEAISQNDIPALIDLPVQYADYAAWQREWLQGSVLDEQLSYWSEK
                     LSGAEPLLTLPTDRSRPAVQTYEGAIYSTTLAGELLDKLQALSRKEESTLFMTLLAAFQ
                     TLLYRYSGQEDILVGSPVAGRNRPETEQMIGFFINTLVLRTDMSGEPTFRDLLARVKET
                     SLEAYAHQDLPFEKLVDELALERSLSYSPLFQVMFVLQNFQLDLDEKAGIRVSEFDMDK
                     HLVTAKYDLTLTMAEKQNGLIATFEYNTALFDETTIVRMSQHFHHLLEAIVQMPEKTIT
                     QLPFLPQAEREQLLVEWNDTTTAYPRDQRVDQLFKQTAMRYPNRIAVVSGDQSLTYSQL
                     ETRSNQVANYLQKQGVRAGSLVGICVKRSLEMLIGVLGILKAGGAYVPLDSDYPKERLA
                     Y"
     source          1..6867
                     /db_xref="taxon:2572910"
                     /geo_loc_name="Italy"
                     /mol_type="genomic DNA"
                     /organism="Brevibacillus sp. AG162"
                     /strain="AG162"
                     /submitter_seqid="Ga0222362_120"
     gene            complement(<1..>6867)
                     /locus_tag="FB479_RS30220"
     region          1..6867
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..6867
                     /SMILES="NC(C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..6867
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:6865](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..6865)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(31..72)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0025"
                     /evalue="7.40E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="FB479_RS30220"
                     /protein_end="2278"
                     /protein_start="2264"
                     /score="19.0"
                     /tool="antismash"
                     /translation="LGILKAGGAYVPLD"
     CDS_motif       complement(601..720)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0024"
                     /evalue="3.20E-25"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="FB479_RS30220"
                     /protein_end="2088"
                     /protein_start="2048"
                     /score="80.6"
                     /tool="antismash"
                     /translation="KETSLEAYAHQDLPFEKLVDELALERSLSYSPLFQVMFVL"
     aSDomain        complement(760..1662)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FB479_RS30220_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.20E-120"
                     /label="FB479_RS30220_Condensation_LCL.1"
                     /locus_tag="FB479_RS30220"
                     /protein_end="2035"
                     /protein_start="1734"
                     /score="393.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="GHLPLSFAQQRLWFLDRLMPDSALYNIPLAIRLHGDLNMEAWNKS
                     LQVLIQRHESLRTTFDHVDGQAVQIIHPYQEQQLRVIDLRELPADECEAETQRLAGREA
                     ATPFNLSEGPLFRTTLIRISEQETVFLLNMHHIISDGWSMGVFIREWFTTYEAISQNDI
                     PALIDLPVQYADYAAWQREWLQGSVLDEQLSYWSEKLSGAEPLLTLPTDRSRPAVQTYE
                     GAIYSTTLAGELLDKLQALSRKEESTLFMTLLAAFQTLLYRYSGQEDILVGSPVAGRNR
                     PETEQMIGFFINTLVLRTDM"
     CDS_motif       complement(769..858)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0023"
                     /evalue="1.60E-18"
                     /label="C5_LCL_267-296"
                     /locus_tag="FB479_RS30220"
                     /protein_end="2032"
                     /protein_start="2002"
                     /score="58.9"
                     /tool="antismash"
                     /translation="DILVGSPVAGRNRPETEQMIGFFINTLVLR"
     CDS_motif       complement(1111..1149)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0022"
                     /evalue="5.50E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1918"
                     /protein_start="1905"
                     /score="18.8"
                     /tool="antismash"
                     /translation="QYADYAAWQREWL"
     CDS_motif       complement(1198..1263)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0021"
                     /evalue="1.10E-11"
                     /label="C3_LCL_132-143"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1889"
                     /protein_start="1867"
                     /score="37.0"
                     /tool="antismash"
                     /translation="MHHIISDGWSMGVFIREWFTTY"
     CDS_motif       complement(1483..1596)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0020"
                     /evalue="4.10E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1794"
                     /protein_start="1756"
                     /score="57.8"
                     /tool="antismash"
                     /translation="ALYNIPLAIRLHGDLNMEAWNKSLQVLIQRHESLRTTF"
     CDS_motif       complement(1615..1647)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0019"
                     /evalue="4.40E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1750"
                     /protein_start="1739"
                     /score="9.7"
                     /tool="antismash"
                     /translation="SFAQQRLWFLD"
     aSDomain        complement(1714..1911)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FB479_RS30220_PCP.2"
                     /evalue="6.40E-27"
                     /label="FB479_RS30220_PCP.2"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1717"
                     /protein_start="1651"
                     /score="85.6"
                     /tool="antismash"
                     /translation="EELVAAIWSHVLGIEKVGIHDNFFELGGHSLLATRVISRLRDMFA
                     GEVPIRTLFENPTVAELSEAL"
     aSModule        complement(1714..4806)
                     /complete
                     /domains="nrpspksdomains_FB479_RS30220_Condensation_DCL.1"
                     /domains="nrpspksdomains_FB479_RS30220_AMP-binding.1"
                     /domains="nrpspksdomains_FB479_RS30220_PCP.2"
                     /locus_tags="FB479_RS30220"
                     /monomer_pairings="Ala -> Ala"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1972..1998)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0018"
                     /evalue="2.70E-01"
                     /label="NRPS-A_a10"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1631"
                     /protein_start="1622"
                     /score="4.5"
                     /tool="antismash"
                     /translation="GKIDRKQLP"
     CDS_motif       complement(2212..2277)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0017"
                     /evalue="8.60E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1551"
                     /protein_start="1529"
                     /score="37.0"
                     /tool="antismash"
                     /translation="YLGRIDHQVKIRGYRIELGELE"
     aSDomain        complement(2242..3420)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FB479_RS30220_AMP-binding.1"
                     /evalue="3.60E-109"
                     /label="FB479_RS30220_AMP-binding.1"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1541"
                     /protein_start="1148"
                     /score="357.0"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="FEAQVAATPDAEALIVGEERLTYAELNRRANQLAHYLRVQGIGPE
                     VLVAVLMERTTEMIVAMMGILKAGGAYVPIDPAYPQERIGYTLEDSQAAILLTQGSLLP
                     MLPEHTAQVICLDRDWESIAEQSEENLLNLVEPTNLSYVIYTSGSTGLPKGVAIQHSSV
                     IAFIAWAKTVFSAEEMSGVLASTSICFDLSVYEIFVTLSFGGKVILADNALHVPSLPAA
                     NEVTLINTVPSAAKELVRMNAIPSTVRVVNLAGEPLPNTLAQSLYALEHVEKVFNLYGP
                     SEDTTYSTYVQVTKGATSEPTIGRPLSNTQAYILDANLQPVPLGLPGELYLGGEGLARE
                     YLNRPELTAERFLSNPFHADPYARMYSTGDLVRYLPDGQIEYLGRIDHQVKIR"
     CDS_motif       complement(2365..2454)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0016"
                     /evalue="3.20E-16"
                     /label="NRPS-A_a6"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1500"
                     /protein_start="1470"
                     /score="51.4"
                     /tool="antismash"
                     /translation="PLGLPGELYLGGEGLAREYLNRPELTAERF"
     CDS_motif       complement(2515..2553)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0015"
                     /evalue="1.40E+01"
                     /label="NRPS-A_a3"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1450"
                     /protein_start="1437"
                     /score="-0.4"
                     /tool="antismash"
                     /translation="YVQVTKGATSEPT"
     CDS_motif       complement(2941..3000)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0014"
                     /evalue="4.30E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1308"
                     /protein_start="1288"
                     /score="28.4"
                     /tool="antismash"
                     /translation="SYVIYTSGSTGLPKGVAIQH"
     CDS_motif       complement(3193..3234)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0013"
                     /evalue="1.70E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1224"
                     /protein_start="1210"
                     /score="18.0"
                     /tool="antismash"
                     /translation="MGILKAGGAYVPID"
     CDS_motif       complement(3748..3867)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0012"
                     /evalue="2.30E-22"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="FB479_RS30220"
                     /protein_end="1039"
                     /protein_start="999"
                     /score="71.5"
                     /tool="antismash"
                     /translation="QKTQLDIRQYEYTPLVDVQGWSAVPHGQSLFDSILVFENY"
     aSDomain        complement(3907..4806)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FB479_RS30220_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="3.40E-117"
                     /label="FB479_RS30220_Condensation_DCL.1"
                     /locus_tag="FB479_RS30220"
                     /protein_end="986"
                     /protein_start="686"
                     /score="382.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHILLDG"
                     /tool="antismash"
                     /translation="ESVYALTPLQEGMLFHSLYEQEGGDYVVQFLMTVRDISLEVFEQA
                     WQKIVDRHSILRTSFIWEGLEQPHQIVRKQVKVSLEKIDLRHVDVDKQEAEIQSYMEKD
                     RVRNFPFNKAPLMRWTLFCTNDNTYRFLWSFHHILLDGWSMPLVLQDWQASYQAIDKGE
                     EEKLEAVQPFSRYIAWLNKQDKQVAERYWREQLKGFYEPTPLAMGKAGSHAIQPKAYRD
                     RTVSLSKEVTAHLQAFARKQQLTINTLVQGAWALILRGYSGSDDVVFGTTVSGRPADLP
                     GVENMVGLFINTLPVRVMF"
     CDS_motif       complement(3916..4011)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0011"
                     /evalue="2.90E-22"
                     /label="C5_DCL_263-294"
                     /locus_tag="FB479_RS30220"
                     /protein_end="983"
                     /protein_start="951"
                     /score="71.1"
                     /tool="antismash"
                     /translation="DVVFGTTVSGRPADLPGVENMVGLFINTLPVR"
     CDS_motif       complement(4339..4404)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0010"
                     /evalue="3.70E-10"
                     /label="C3_DCL_135-156"
                     /locus_tag="FB479_RS30220"
                     /protein_end="842"
                     /protein_start="820"
                     /score="32.2"
                     /tool="antismash"
                     /translation="FHHILLDGWSMPLVLQDWQASY"
     CDS_motif       complement(4621..4731)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0009"
                     /evalue="6.70E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="FB479_RS30220"
                     /protein_end="748"
                     /protein_start="711"
                     /score="44.1"
                     /tool="antismash"
                     /translation="YVVQFLMTVRDISLEVFEQAWQKIVDRHSILRTSFIW"
     CDS_motif       complement(4759..4797)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0008"
                     /evalue="7.60E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="FB479_RS30220"
                     /protein_end="702"
                     /protein_start="689"
                     /score="15.4"
                     /tool="antismash"
                     /translation="YALTPLQEGMLFH"
     aSDomain        complement(4831..5292)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FB479_RS30220_TIGR01720.1"
                     /evalue="5.80E-46"
                     /label="FB479_RS30220_TIGR01720.1"
                     /locus_tag="FB479_RS30220"
                     /protein_end="678"
                     /protein_start="524"
                     /score="148.1"
                     /tool="antismash"
                     /translation="GHTLKAVKEQLRQIPNKGVGYGILRYLCDDLELQHRLNAKPKPQI
                     SFNYLGQFDQTVAVSSTFGIASECSGANLAPDSIRENLLDIISAVTGDQLHVTWLYNEN
                     IHDEQTIAAVADDYMEALREIMSYTQAAEEIGYTPSDFPLANLNQQSIDK"
     aSModule        complement(4831..6474)
                     /domains="nrpspksdomains_FB479_RS30220_PCP.1"
                     /domains="nrpspksdomains_FB479_RS30220_Epimerization.1"
                     /domains="nrpspksdomains_FB479_RS30220_TIGR01720.1"
                     /incomplete
                     /locus_tags="FB479_RS30220"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(5212..5274)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0007"
                     /evalue="2.20E-12"
                     /label="NRPS-E6"
                     /locus_tag="FB479_RS30220"
                     /protein_end="551"
                     /protein_start="530"
                     /score="38.9"
                     /tool="antismash"
                     /translation="VKEQLRQIPNKGVGYGILRYL"
     aSDomain        complement(5311..6225)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FB479_RS30220_Epimerization.1"
                     /evalue="6.70E-161"
                     /label="FB479_RS30220_Epimerization.1"
                     /locus_tag="FB479_RS30220"
                     /protein_end="518"
                     /protein_start="213"
                     /score="528.7"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="TGNVLLTPIQKWFFAANQPSIHHWNQSFLLTVEEPVDTNALERAI
                     TELLAHHDALRMRYTNNDGLWSQHIEGLGEPVPFHLVDLADLSSEEQFARLEEIAGEMQ
                     ASLNITEGPLFRAVYFHLGKERAGRLLLVVHHLVVDGVSWRILLDDLQLAYEQAVNGQT
                     VQFPSKTTSFKAWAEQLHAYAKSDSLEKEKAFWANQAYEVDPLPVDRTFEPAQNTEAST
                     KQITLTLTAEETRNLLHETLPSYRLQINDVLLASLAKALNRWTGKETMVVTLEGHGREE
                     IIEGADLSRTVGWFTSMYPIQIST"
     CDS_motif       complement(5320..5367)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0006"
                     /evalue="5.50E-09"
                     /label="NRPS-E5"
                     /locus_tag="FB479_RS30220"
                     /protein_end="515"
                     /protein_start="499"
                     /score="28.0"
                     /tool="antismash"
                     /translation="DLSRTVGWFTSMYPIQ"
     CDS_motif       complement(5773..5838)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0005"
                     /evalue="9.20E-11"
                     /label="NRPS-E2"
                     /locus_tag="FB479_RS30220"
                     /protein_end="364"
                     /protein_start="342"
                     /score="33.9"
                     /tool="antismash"
                     /translation="LLLVVHHLVVDGVSWRILLDDL"
     CDS_motif       complement(6049..6156)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0004"
                     /evalue="3.80E-08"
                     /label="C2_LCL_024-062"
                     /locus_tag="FB479_RS30220"
                     /protein_end="272"
                     /protein_start="236"
                     /score="25.7"
                     /tool="antismash"
                     /translation="WNQSFLLTVEEPVDTNALERAITELLAHHDALRMRY"
     CDS_motif       complement(6181..6210)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0003"
                     /evalue="1.10E-03"
                     /label="NRPS-E1"
                     /locus_tag="FB479_RS30220"
                     /protein_end="228"
                     /protein_start="218"
                     /score="12.0"
                     /tool="antismash"
                     /translation="LTPIQKWFFA"
     aSDomain        complement(6271..6474)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FB479_RS30220_PCP.1"
                     /evalue="4.40E-24"
                     /label="FB479_RS30220_PCP.1"
                     /locus_tag="FB479_RS30220"
                     /protein_end="198"
                     /protein_start="130"
                     /score="76.5"
                     /tool="antismash"
                     /translation="ERKLSEIWQDVLRIEEISIHSNFFELGGDSILSIQIVSRANQAGI
                     RLTPKQIFENQTIAELAALVSTT"
     CDS_motif       complement(6532..6558)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0002"
                     /evalue="1.40E-01"
                     /label="NRPS-A_a10"
                     /locus_tag="FB479_RS30220"
                     /protein_end="111"
                     /protein_start="102"
                     /score="5.3"
                     /tool="antismash"
                     /translation="GKVDRRALP"
     CDS_motif       complement(6772..6837)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FB479_RS30220_0001"
                     /evalue="4.20E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="FB479_RS30220"
                     /protein_end="31"
                     /protein_start="9"
                     /score="34.8"
                     /tool="antismash"
                     /translation="FIGRIDNQVKIRGFRIELSEVE"
ORIGIN
        1 atgtatgcca gacgttcttt cggataatcc gaatcgagcg gtacataagc gccaccagct
       61 ttgagaatcc caagcacccc gatcagcatt tcgagagaac gcttcacgca gataccgacg
      121 agtgaacctg cgcgaacgcc ttgtttttgc aggtagttgg ctacttggtt ggatcgtgtc
      181 tctagctggg agtatgtgag actctggtct cccgatacca ctgcaatgcg attcgggtaa
      241 cgcatcgcgg tctgcttgaa caattgatcc acgcgttgat cccgcgggta agccgtcgtg
      301 gtgtcgttcc attccaccag cagctgctcc cgttctgcct gaggcaaaaa cggcaattgg
      361 gtaatggtct tctccggcat ctgaacgatg gcttcgagta agtgatggaa gtgctgtgac
      421 atgcgtacaa tcgtcgtttc atcaaacagc gctgtattgt actcaaacgt tgcgatcaat
      481 ccgttttgtt tttctgccat cgtcaacgtc aggtcgtatt tggccgtgac gaggtgtttg
      541 tccatatcga attccgatac gcgaatacca gccttttcgt ctaagtctag ctggaagttt
      601 tggaggacaa acatgacttg gaacagtggc gagtagctga gactacgctc tagcgccagt
      661 tcatcgacca atttttcgaa aggaagatcc tgatgggcat aggcttctag cgacgtctcc
      721 tttactcttg ccagcaagtc acggaatgtc ggctcccctg acatatccgt acgcagcacc
      781 aatgtattaa tgaagaatcc aatcatttgt tccgtctctg gtctattgcg tcctgcgaca
      841 gggcttccga cgaggatgtc ttcctgtcca ctgtagcggt agagcaaagt ttggaaggct
      901 gccagcaagg tcatgaacag cgtcgattct tctttacggc taagtgcttg taatttgtcg
      961 agaagttcac ctgcgagcgt cgttgaataa atcgctccct cgtacgtttg tacagcagga
     1021 cgtgaacggt ctgtcggcaa ggtgagaagc ggttccgcac cacttaattt ttcactccag
     1081 tacgaaagct gttcgtctaa taccgaaccc tggagccact cgcgttgcca agccgcgtag
     1141 tcagcatatt gcacaggtaa atcaatcaat gctggtatgt cgttttggct aatcgcttcg
     1201 taggtggtga accattcgcg aatgaatact cccatcgacc aaccgtcaga gatgatgtga
     1261 tgcatgttca aaaggaagac cgtttcttgc tcgcttatgc gaatcaaggt ggtacggaac
     1321 aacggacctt cgctcaggtt gaatggcgta gctgcctctc ttcctgccaa acgctgtgtt
     1381 tctgcttcac actcgtccgc tggtagctcc cgcaagtcga tgacacgtaa ctgctgctct
     1441 tggtaaggat gtatgatttg cacggcttgc ccatctacgt ggtcaaaggt tgtccgcaag
     1501 ctttcatggc gctggatcag tacctgcaag ctcttgttcc aagcctccat attcaggtcc
     1561 ccgtgcaatc gaatcgctaa cgggatgttg tacaaggcgc tgtctggcat cagacgatcc
     1621 aggaaccaca ggcgctgctg tgcaaaggac agtggtagat gaccttcccg tgaaacaggt
     1681 tcgatcgagg cacctgtttc ttgcaaaaag ctaccgagtg cttcggacag ttctgcaacg
     1741 gttgggtttt cgaacagagt ccggataggt acttctcctg caaacatgtc acgaagacgg
     1801 gaaataaccc gtgtcgctaa gagagaatgt ccgccaagct cgaagaagtt gtcatgaatc
     1861 ccgacttttt cgatgccgag cacgtgcgac cagatcgcgg cgaccagctc ctctgtttgg
     1921 ttgcgtggcg ctacatattc ttgtgtagaa accatctgcc cccactccgg ctctggcagt
     1981 tgcttccggt cgatcttgcc gttcggtgtg agcggcatag cttccagcca gacaaagacg
     2041 gatggaacca taaactccgg caattttgct tttgcccaga cagctaagtc agtagcctct
     2101 tccctatttt cctcttttgt cgtcacatac gccgccagac gtttgtcccc caactggtct
     2161 tctcttgccg tcaccacagc ttccttgaca gccggatggg tgcgaaggac agcctccaac
     2221 tcccctaact cgatccgata gccgcgaatc ttcacctgat ggtcaatccg gcccagatac
     2281 tcgatttgtc catctgggag ataacggacc aagtcaccgg tgctgtacat ccgtgcatac
     2341 gggtcagcgt ggaatggatt ggacaagaat cgttctgctg tcagctctgg acgattcagg
     2401 tactcgcgag ccaatccttc cccgccaaga tacagttccc caggtaaacc caacggtaca
     2461 ggttgcaggt tggcatcgag aatatacgct tgtgtgttgg acaaaggacg gccaatcgtt
     2521 ggttctgaag tggcgccttt ggtgacctgc acatatgtgg agtaggtcgt atcttcagac
     2581 ggaccataga gattgaagac tttttccaca tgctccagtg catatagact ttgcgccaat
     2641 gtgtttggca gtggctctcc agcaaggttg accactcgta ccgtcgacgg gatcgcgttc
     2701 atccgcacca gttctttggc cgccgatgga acggtattga ttaaggtcac ctcgttggct
     2761 gctggcaggc tcggtacatg caaggcgttg tcagccagga tgactttccc gccgaagctt
     2821 aaggtcacga atatttcata gacagacagg tcgaagcaga tagaagtaga ggcgagcacc
     2881 ccactcattt cttcagccga aaagacggtt ttcgcccaag cgataaaggc aatgacacta
     2941 ctgtgctgaa tcgctactcc tttgggcaga cctgtcgagc ccgaggtgta gatgacatag
     3001 gaaaggttcg ttggctccac cagattaagc aggttctctt cgctttgctc cgcgatagac
     3061 tcccagtcac gatccaggca gatcacttgt gcggtatgtt caggcagcat cggtagcagg
     3121 cttccttggg tcaacaggat tgccgcttgc gaatcctcca acgtatagcc gattctctcc
     3181 tgtggatacg cgggatcaat tggtacatac gcgccgccag ctttgagaat tcccatcatc
     3241 gctacgatca tctcggttgt acgttccatg agaacagcga ccaagacctc tggaccgatc
     3301 ccttgcaccc gcagatagtg agccaactgg tttgctcggc ggttcagctc ggcgtacgtc
     3361 agtctttctt ctccgacgat taaagcctcc gcatccggtg tagcggcgac ttgtgcttca
     3421 aacagctggt gcatcaccgc gtcacgagta tattccgttt ccgtcgcgtt ccattccact
     3481 aacagcttgt ggcgttcctc gtccgtaata aatgaaagtg tacgcaacgg ttgttccgct
     3541 tgggcagtta gggcatgtag aacctgcgac atctgttcta ataccttacc aatcacttcc
     3601 gtatcaaaac gcccttgctc atagatcaat ttgagagcaa gttcatcgcc aggagccgcc
     3661 accaatgtca aggggtaatt ggtttgctcc actgcttgga cttcactcag cgacactccg
     3721 gaatctgaat cctcacttgt actgttcagg tagttctcaa aaacgagaat actgtcgaac
     3781 aacgattgtc catgtgggac cgcgctccag ccttgaacgt ctacaagtgg tgtatattcg
     3841 tattggcgga tgtctaattg tgtcttttgc aagtgttgta accattcctg tatcgtttgg
     3901 cttgggtcaa acatgacgcg gacaggtagc gtattaataa agagtcctac catgttttcc
     3961 actcctggca ggtctgctgg acgaccagaa actgttgttc caaagacaac atcgtctgat
     4021 ccgctgtaac ctctcaggat gagtgcccat gccccttgca ccaacgtatt tatcgtcaac
     4081 tgctgcttgc gggcaaaagc ttgcaggtgg gccgttactt ccttggataa ggaaacggtt
     4141 cgatctctgt aggctttagg ctgtatggca tgactgcctg cttttcccat agcaagtggt
     4201 gttggctcgt agaagccttt taactgctcg cgccaatagc gctccgcaac ttgtttatct
     4261 tgcttattga gccatgcgat atagcggctg aatggttgga ctgcttctaa tttttcttct
     4321 tcgcctttat cgatcgcttg gtaacttgcc tgccaatctt gcaagacaag tggcatactc
     4381 cagccatcta gcaaaatatg atggaagctc cataagaagc gatacgtatt gtcgttcgtg
     4441 caaaacaatg tccaacgcat caatggcgcc ttattaaacg ggaagtttcg cacccggtct
     4501 ttttccatgt acgattgaat ttcagcttcc tgcttatcga catccacatg gcgtaggtcg
     4561 attttctcta agctcacttt tacttgtttt cgaacgattt gatgcggttg ttccaatcct
     4621 tcccaaatga aggaggtacg aagaatcgaa tggcggtcga ctattttctg ccatgcttgc
     4681 tcgaatactt ccaagctaat atctcgcact gtcatgagga actgtacaac ataatcgccg
     4741 ccctcttgtt catacaggga gtggaacagc atcccctctt gcagtggtgt cagcgcgtat
     4801 acactttcga tctgccgatc cgaagcgatg tatttgtcga tagactgctg atttaggtta
     4861 gccagcggga agtctgacgg tgtgtagccg atttcctctg ccgcttgcgt gtaagacata
     4921 atctcccgca gtgcttccat gtaatcatca gcaaccgccg caatcgtctg ttcgtcatgg
     4981 atgttttcgt tgtacaacca ggtgacgtgc agctgatctc ctgtaaccgc actaatgata
     5041 tctaacaagt tctcccgaat cgaatcaggg gcaagattgg ctccactgca ttcagacgct
     5101 atgccaaacg tagaggaaac cgccaccgtc tgatcaaatt gtccaaggta gttgaagctg
     5161 atttgcggtt tcggtttggc gttcagtcgg tgttgtagtt caagatcatc gcatagatag
     5221 cgcaaaattc catagccgac acccttattt ggaatctggc ggagctgttc cttgactgcc
     5281 ttcagcgtat gtccccaagg tttttcttgc tcggtagaaa tctgtatcgg gtacatactt
     5341 gtgaaccagc caaccgtacg tgataaatct gctccctcga taatctcctc gcgtccatgt
     5401 ccttccagag ttacgaccat cgtctctttt cctgtccaac gattcagcgc ttttgccaag
     5461 gaagcaagca atacgtcatt gatttgcaga cggtaggaag ggagcgtttc gtgaagcaag
     5521 ttgcgcgtct cttcggcggt cagcgtcagt gtaatctgtt tcgtggatgc ctcggtgttt
     5581 tgagctggct caaacgtacg gtcgacagga agtggatcca cttcatacgc ttgatttgcc
     5641 cagaaggctt tttccttttc aagcgaatcg gacttggcat aagcgtgtaa ctgttccgcc
     5701 catgctttaa acgaggtcgt ttttgacggg aattgcaccg tctgaccatt tacagcttgc
     5761 tcataggcca actgcaagtc atccagcaaa atgcgccaag ataccccgtc gactaccaag
     5821 tgatgaacaa cgagcaggag gcgtcccgcg cgttcttttc caaggtggaa gtacacagct
     5881 cggaacagag ggccttctgt gatgttcaga ctcgcttgca tctcgcctgc aatttcttcc
     5941 agtcgcgcga attgctcctc tgatgagaga tcagccaaat caaccaaatg gaaaggtaca
     6001 ggttcgccta agccctcgat gtgttgtgac cataaaccgt cgttgttggt ataacgcatg
     6061 cgtaaagcat catggtgtgc aagcaactcg gtaatggcac gctccaatgc gttcgtatca
     6121 accggttcct cgactgtcaa caagaaggat tgattccagt gatggataga cggttgattt
     6181 gcggcaaaga accacttctg aattggcgta agcaacacat ttccggtcac gaggccttgt
     6241 tccgccagtt gaatagcatt ttccccgttt ccagttgttg aaacgagagc tgccagttca
     6301 gcgattgttt ggttttcaaa gatctgttta ggtgtaagac gaatccctgc ttggttagcc
     6361 cgagagacga tttggatact caggatcgag tcccctccga gttcgaagaa gttggaatgg
     6421 atactgattt cttcgattct gagaacgtct tgccaaatct cagacagttt gcgttcgata
     6481 aaggtatgtg gtgcaacgta gtccccatca cttgtgttcg catagtcagg ttttggcagg
     6541 gcccggcggt ctactttccc gtttggcgta agcggcaatt catccagcat gacaaaagca
     6601 gatggcacca tataatccgg aacatgtgct ttgaagtgtt ggcgcagttc ggctatgtca
     6661 acctcttgct ccgcttgctt gaccgcgtaa gcgacgagct gtttcttgcc cggttcatct
     6721 tcgcggacaa tgacgacgga ggcggcaaga gcgtggtgtt tggctaaaac gaactctact
     6781 tcacttagct cgatgcggaa tccgcggatt tttacctgat tgtcgattcg gccgatgaat
     6841 tcgatcagac catctggcag gtaacga
//
