LOCUS NZ_VFNO01000010 10384 bp DNA linear CON 16-AUG-2024 DEFINITION Brevibacillus sp. AG162 Ga0222362_110, whole genome shotgun sequence. ACCESSION NZ_VFNO01000010 VERSION NZ_VFNO01000010.1 KEYWORDS WGS; RefSeq. SOURCE Brevibacillus sp. AG162 ORGANISM Brevibacillus sp. AG162 Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus. REFERENCE 1 (bases 1 to 201887) AUTHORS Venturi,V. TITLE Genome sequencing of rice bacterial endophytes JOURNAL Unpublished REFERENCE 2 (bases 1 to 201887) AUTHORS Venturi,V., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K., Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C., Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T. TITLE Direct Submission JOURNAL Submitted (14-JUN-2019) Prokaryotic Super Group, DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12.0 Genome Representation :: Full Expected Final Version :: No Genome Coverage :: 236.0x Sequencing Technology :: Illumina HiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_006715575.1-RS_2024_08_16 Annotation Date :: 08/16/2024 02:19:50 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.8 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 6,054 CDSs (total) :: 5,923 Genes (coding) :: 5,868 CDSs (with protein) :: 5,868 Genes (RNA) :: 131 rRNAs :: 1, 6, 5 (5S, 16S, 23S) complete rRNAs :: 1 (5S) partial rRNAs :: 6, 5 (16S, 23S) tRNAs :: 111 ncRNAs :: 8 Pseudo Genes (total) :: 55 CDSs (without protein) :: 55 Pseudo Genes (ambiguous residues) :: 0 of 55 Pseudo Genes (frameshifted) :: 25 of 55 Pseudo Genes (incomplete) :: 38 of 55 Pseudo Genes (internal stop) :: 3 of 55 Pseudo Genes (multiple problems) :: 11 of 55 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 15:50:39 NOTE :: This is a single region extracted from a larger record! Orig. start :: 70116 Orig. end :: 80500 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to VFNO01000010.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ FEATURES Location/Qualifiers region 1..10384 /candidate_cluster_numbers="1" /contig_edge="False" /product="ectoine" /region_number="2" /rules="ectoine_synt" /tool="antismash" cand_cluster 1..10384 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="ectoine_synt" /kind="single" /product="ectoine" /protoclusters="1" /tool="antismash" protocluster 1..10384 /aStool="rule-based-clusters" /category="other" /contig_edge="False" /core_location="[5000:5384](+)" /cutoff="20000" /detection_rule="ectoine_synt" /neighbourhood="5000" /product="ectoine" /protocluster_number="1" /tool="antismash" proto_core 5001..5384 /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="ectoine_synt" /neighbourhood="5000" /product="ectoine" /protocluster_number="1" gene 125..1039 /gene="ehuB" /locus_tag="FB479_RS24255" /old_locus_tag="FB479_11036" CDS 125..1039 /GO_component="GO:0030288 - outer membrane-bounded periplasmic space [Evidence IEA]" /GO_function="GO:0033286 - ATPase-coupled ectoine transmembrane transporter activity [Evidence IEA]" /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine transmembrane transporter activity [Evidence IEA]" /GO_function="GO:0033294 - ectoine binding [Evidence IEA]" /GO_function="GO:0033295 - hydroxyectoine binding [Evidence IEA]" /GO_process="GO:0033308 - hydroxyectoine transmembrane transport [Evidence IEA]" /GO_process="GO:0051470 - ectoine transmembrane transport [Evidence IEA]" /codon_start=1 /gene="ehuB" /gene_functions="biosynthetic-additional (smcogs) SMCOG1091: glutamine-binding lipoprotein glnH" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007506238.1" /locus_tag="FB479_RS24255" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11036" /product="ectoine/hydroxyectoine ABC transporter substrate-binding protein EhuB" /protein_id="WP_255438596.1" /transl_table=11 /translation="MTKTSIARWGAMAILTCVLLLGGCGAGTQEAQQVAGGNTTESGQD TLKKAKSQGFVTVGFANEKPYAYASPDGKLTGEAVEVARVILKRMGINEMNGVLTEFGS LIPGLKAKRFDIITAGMYITPKRAQEVAFANPEYSIGEALAVKKGNPLGLKSYKDIAAK PQAKVAVMSGGIETEYLEKSGVKKEQMVLVPDQPSAIAALQAGRADAITMTGPSLQAML ESAKDDQLERVMDFVQPEIEGKSVRGYGAAAFRKEDEAFVKAYNEELQKLKESGELLEI LKPFGFTEQELPGDMTANELLQQ" gene 1054..1695 /gene="ehuC" /locus_tag="FB479_RS24260" /old_locus_tag="FB479_11037" CDS 1054..1695 /GO_component="GO:0005886 - plasma membrane [Evidence IEA]" /GO_function="GO:0033286 - ATPase-coupled ectoine transmembrane transporter activity [Evidence IEA]" /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine transmembrane transporter activity [Evidence IEA]" /GO_process="GO:0033308 - hydroxyectoine transmembrane transport [Evidence IEA]" /GO_process="GO:0051470 - ectoine transmembrane transport [Evidence IEA]" /codon_start=1 /gene="ehuC" /gene_functions="transport (smcogs) SMCOG1096: polar amino acid ABC transporter, inner membrane" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017754763.1" /locus_tag="FB479_RS24260" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11037" /product="ectoine/hydroxyectoine ABC transporter permease subunit EhuC" /protein_id="WP_142066987.1" /transl_table=11 /translation="MSDLLPLLAKGAGTTLQVTVLSVLVALFVAFLAGFGRLSRWSGIR IVSTVYVELFRGTSLLVQLFWLFYALPLLEIELPPLAAGVIALGLNYGAYGSEVVRSSI QAIPKGQTEAAIALNMTAWQRMKLVILPQALVMMLPAFGNLLIELLKGTSLISLITLAD LTFQGMILRTTDSSRTFEIFFVILVMYFLMAYPLTLAMRWLERRFHAGRA" gene 1701..2363 /gene="ehuD" /locus_tag="FB479_RS24265" /old_locus_tag="FB479_11038" CDS 1701..2363 /GO_component="GO:0005886 - plasma membrane [Evidence IEA]" /GO_function="GO:0033286 - ATPase-coupled ectoine transmembrane transporter activity [Evidence IEA]" /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine transmembrane transporter activity [Evidence IEA]" /GO_process="GO:0033308 - hydroxyectoine transmembrane transport [Evidence IEA]" /GO_process="GO:0051470 - ectoine transmembrane transport [Evidence IEA]" /codon_start=1 /gene="ehuD" /gene_functions="transport (smcogs) SMCOG1096: polar amino acid ABC transporter, inner membrane" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007506236.1" /locus_tag="FB479_RS24265" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11038" /product="ectoine/hydroxyectoine ABC transporter permease subunit EhuD" /protein_id="WP_142066988.1" /transl_table=11 /translation="MWSWSFTWSILPEILQAIQTTIVATLAGFVLAMVGGLVLAFGRRS KRRVLSWSSAAFIEFVRSTPLLVQLYFLFYGLPAIGISLGPLTSGVLGLGIHYSCYLSE VYRSGIDAVPRGQWEAARALNFSIGQTWLRIILPQAIRPVVPVLGNYLIVMFKETPILS AITLVEMLATAKIIGAESFRYLEAFTVVGLLFLVLSYGSSLLIKKWEQSWNKQYMQR" gene 2369..3157 /gene="ehuA" /locus_tag="FB479_RS24270" /old_locus_tag="FB479_11039" CDS 2369..3157 /GO_component="GO:0009898 - cytoplasmic side of plasma membrane [Evidence IEA]" /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_function="GO:0033286 - ATPase-coupled ectoine transmembrane transporter activity [Evidence IEA]" /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine transmembrane transporter activity [Evidence IEA]" /GO_process="GO:0033308 - hydroxyectoine transmembrane transport [Evidence IEA]" /GO_process="GO:0051470 - ectoine transmembrane transport [Evidence IEA]" /codon_start=1 /gene="ehuA" /gene_functions="transport (smcogs) SMCOG1000: ABC transporter ATP-binding protein" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007131784.1" /locus_tag="FB479_RS24270" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11039" /product="ectoine/hydroxyectoine ABC transporter ATP-binding protein EhuA" /protein_id="WP_106655350.1" /transl_table=11 /translation="MKTGIQTEPVVRYRNVSKAYGDLQILKGIDLDIHPGEKIAVIGPS GSGKTTLARILMTLEEPTEGTVEVAGQNLWQKQVSGRLVKADEKHLHSIRGNIGMVFQH FNLFPHMTILRNVTEAPIHVLGMSKEEAKERAVTMLKKVGLDDKLAAYPSQLSGGQKQR VAIARALVMRPKVMLFDEVTSALDPELVGEVLAVIKEIAREGEMAMLLITHEMDFARDV ADRVIFTDGGTIVEQGSPEQIFYNPQSPRLQAFLSRFRAG" gene 3176..3685 /gene="ectA" /locus_tag="FB479_RS24275" /old_locus_tag="FB479_11040" CDS 3176..3685 /EC_number="2.3.1.178" /GO_function="GO:0033816 - diaminobutyrate acetyltransferase activity [Evidence IEA]" /GO_process="GO:0019491 - ectoine biosynthetic process [Evidence IEA]" /codon_start=1 /gene="ectA" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_008589527.1" /locus_tag="FB479_RS24275" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11040" /product="diaminobutyrate acetyltransferase" /protein_id="WP_255438597.1" /transl_table=11 /translation="MKATSRDRIVLRLPTEEDGASMWELARDSKTLDLNSSYAYLLFAK YFSDTCLIAEEEGKAVGFVMGFHPPESPDTLFVWQIGVSESKRGQGIAKAMLRTMLQRT AKSDVRFLEATVSPSNGASRALFTKIAKELATECEVTECFPARLFPGRTHEPEWTYRIG PVPTIS" gene 3697..5004 /gene="ectB" /locus_tag="FB479_RS24280" /old_locus_tag="FB479_11041" CDS 3697..5004 /EC_number="2.6.1.76" /GO_function="GO:0030170 - pyridoxal phosphate binding [Evidence IEA]" /GO_function="GO:0047307 - diaminobutyrate-pyruvate transaminase activity [Evidence IEA]" /GO_process="GO:0019491 - ectoine biosynthetic process [Evidence IEA]" /NRPS_PKS="Domain: Aminotran_3 (31-365). E-value: 5.2e-83. Score: 270.8. Matches aSDomain: nrpspksdomains_FB479_RS24280_Aminotran_3.1" /codon_start=1 /gene="ectB" /gene_functions="biosynthetic-additional (rule-based-clusters) Aminotran_3" /gene_functions="biosynthetic-additional (smcogs) SMCOG1013: aminotransferase class-III" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019536117.1" /locus_tag="FB479_RS24280" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11041" /product="diaminobutyrate--2-oxoglutarate transaminase" /protein_id="WP_106655351.1" /sec_met_domain="Aminotran_3 (E-value: 1.1e-86, bitscore: 284.3, seeds: 13, tool: rule-based-clusters)" /transl_table=11 /translation="MKTIVETRSHLSVFDQMESEVRSYCRSFPVVFTKASGHTVWDRQG KEYIDFFAGAGALNYGHNNPAIKKKLMEYLQQDGITHSLDMATEAKEALLTQFHESILT PRGLSYKVMFPGPTGTNSVEAALKLARKATGREMIMSFTNGFHGMTLGSLAATGNRMKR KGAGVPLSHVMALPFDKYMGEGIDTTVYIERLLEDDGSGVPLPAAMILETVQGEGGIHV ASASWLRQIERICRKHDILLIVDDVQAGCGRTGPFFSFEEADITPDIVCLSKSIGGFGL PFSLTLLKPELDVWLPGEHNGTFRGNNLSFIAAAEALEYWRDDSLSESVKQKGSIVQTH LSHLVQTYPDLSGEVRGKGLMQGIAVGVDGLAEEICTRAFQRGLIMETSGPKSEVMKVM PPLTITEEALRKGLNMLDEAVKECLSGDFRKRSEGK" misc_feature complement(3698..3712) /note="TFBS match to ZuR_variant_1, Zinc-responsive repressor, confidence: medium, score: 21.91" /tool="antismash" aSDomain 3790..4791 /aSDomain="Aminotran_3" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_FB479_RS24280_Aminotran_3.1" /evalue="5.20E-83" /label="FB479_RS24280_Aminotran_3.1" /locus_tag="FB479_RS24280" /protein_end="365" /protein_start="31" /score="270.8" /tool="antismash" /translation="FTKASGHTVWDRQGKEYIDFFAGAGALNYGHNNPAIKKKLMEYLQ QDGITHSLDMATEAKEALLTQFHESILTPRGLSYKVMFPGPTGTNSVEAALKLARKATG REMIMSFTNGFHGMTLGSLAATGNRMKRKGAGVPLSHVMALPFDKYMGEGIDTTVYIER LLEDDGSGVPLPAAMILETVQGEGGIHVASASWLRQIERICRKHDILLIVDDVQAGCGR TGPFFSFEEADITPDIVCLSKSIGGFGLPFSLTLLKPELDVWLPGEHNGTFRGNNLSFI AAAEALEYWRDDSLSESVKQKGSIVQTHLSHLVQTYPDLSGEVRGKGLMQGIA" gene 5001..5384 /locus_tag="FB479_RS24285" /old_locus_tag="FB479_11042" CDS 5001..5384 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) ectoine: ectoine_synt" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_019536118.1" /locus_tag="FB479_RS24285" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11042" /product="ectoine synthase" /protein_id="WP_142066989.1" /sec_met_domain="ectoine_synt (E-value: 1.7e-53, bitscore: 173.2, seeds: 10, tool: rule-based-clusters)" /transl_table=11 /translation="MIVKHLEEIMGTKDDIDTPTWSSRRMLLKKDSMGFSMHDTIIKAG TETFIWYKNHLEAVYCIEGEGEIEVVGGETYQIKPGMMYALSENEKHYLRARSQMRMVC VFNPPLTGREVHDEEGAYPLLEV" misc_feature complement(5388..5402) /note="TFBS match to SypG, Regulator of biofilm formation and host colonization, confidence: weak, score: 20.9" /tool="antismash" gene 5415..6311 /gene="thpD" /locus_tag="FB479_RS24290" /old_locus_tag="FB479_11043" CDS 5415..6311 /EC_number="1.14.11.55" /GO_function="GO:0016706 - 2-oxoglutarate-dependent dioxygenase activity [Evidence IEA]" /codon_start=1 /gene="thpD" /gene_functions="biosynthetic-additional (rule-based-clusters) PhyH" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_010269269.1" /locus_tag="FB479_RS24290" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11043" /product="ectoine hydroxylase" /protein_id="WP_142066990.1" /sec_met_domain="PhyH (E-value: 2.7e-34, bitscore: 112.6, seeds: 39, tool: rule-based-clusters)" /transl_table=11 /translation="MKQSRPELLIEDQYPSRIHEHPALLQRVDPVVYWNEKTKEGPLTQ ESLQSYENKGYISLRGFFSPEEVQELRDELTKLWQENRGSTRKEVICEPHSNEVRSIFA VHRDNLVFQHLSRHPRLVSIAQQILGSEVYIHQSRINYKHGFTGKEFYWHSDFETWHVE DGMPRMRALSCSISLEENYHYNGPLLLLPGSHQTFVSCVGQTPESHYEHSLRKQEYGVP DQGSLKKMVEQHGIDTMIGPAGSVVFFDCNTMHGSNGNITPMPRSNVFMVYNSVENRLV APFSGQKPRPDYIATRD" gene 6355..7566 /locus_tag="FB479_RS24295" /old_locus_tag="FB479_11044" CDS 6355..7566 /EC_number="2.7.2.4" /GO_function="GO:0004072 - aspartate kinase activity [Evidence IEA]" /GO_process="GO:0009089 - lysine biosynthetic process via diaminopimelate [Evidence IEA]" /codon_start=1 /inference="COORDINATES: protein motif:HMM:NF005155.0" /locus_tag="FB479_RS24295" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11044" /product="aspartate kinase" /protein_id="WP_142066991.1" /transl_table=11 /translation="MALVVQKYGGTSVACVERIRAVAQRIAQSRQSGNKVVVVLSAMGK TTDRLLKMATDIDPCPEGRELDMLVSTGEQAAAALLSIALRQQGCNAISLAGWQAGILT EAKHTDAQIVHIDPTPIERRLAEGKVVVVAGFQGVSADGEITTLGRGGSDTSAVALAGS LGAEICEIFTDVEGVYTADPRTVPTAQKIHRITYSDMLELARSGAGVLHPRSVEEAMKK QVPLVVRSSFTEREGTYVGQNERSWSAQQVCGIAHQEKMSVLCIKRKSYSTDHVDTELT EILHEADIYAEVWARGKNSLDICLPTYQVNPLLALLQVKVDGGVDFWREELEWTKINII GVNHSSIQELMQKARDFTFPIRWGRQGTKGASCLVPQKFSKRAIQALHTGLGLDAKMKA IVGS" misc_feature 6382..6395 /note="TFBS match to RpoN, Regulator of nitrogen assimilation and virulence, confidence: medium, score: 16.82" /tool="antismash" gene complement(7624..8217) /locus_tag="FB479_RS24300" /old_locus_tag="FB479_11045" CDS complement(7624..8217) /GO_component="GO:0016020 - membrane [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016739276.1" /locus_tag="FB479_RS24300" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11045" /product="DUF421 domain-containing protein" /protein_id="WP_142066992.1" /transl_table=11 /translation="MSWILIMQTVGVIVFGTLLLRIAGRKSISQMTMAQVVVMIGIGTL LIQPISGHGYWPTFVLALVLVIGMIAIEYLGVKSNLFESIITGKAVLVIENGQLLIENM RKLRLSTDKLEMRLRQAGISSISDVEWATIEVSGNLGYQLKSDKQPATKEDIQKLIHFI ENRIPMVRRDLTVEDNIFSEIKYGHQTTHSDNLQ" misc_feature complement(8295..8309) /note="TFBS match to MexT, Global virulence regulator, confidence: medium, score: 18.58" /tool="antismash" misc_feature 8390..8406 /note="TFBS match to NuR, Nickel-responsive regulator, confidence: weak, score: 22.12" /tool="antismash" gene 8537..8833 /locus_tag="FB479_RS24305" /old_locus_tag="FB479_11046" CDS 8537..8833 /codon_start=1 /inference="COORDINATES: protein motif:HMM:NF019490.4" /locus_tag="FB479_RS24305" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_11046" /product="spore coat protein" /protein_id="WP_255438598.1" /transl_table=11 /translation="MKAEYLGKAAGPTSKLPVPEIQPVLQSFIQSPVPPSPVTPRTTVQ QFNDREIATAYLLTLKRAGREYAWATMEMSHPEIRSFLEDAFRMSSHHAYDVW" misc_feature 9035..9050 /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: weak, score: 20.82" /tool="antismash" misc_feature complement(10308..10321) /note="TFBS match to MogR, Repressor of flagellar motility genes, confidence: weak, score: 19.94" /tool="antismash" ORIGIN 1 gcatgcgtat ttagagaaat cagtcggcgc attttgcttt tccgtcaggg atggccctcc 61 tgaagcggaa gcgatgtgcc gacttttttt ctaccatttc tcataaaaga gaggggcaag 121 aggaatgaca aagactagca tagcgagatg gggggctatg gcgatcctga cgtgtgtctt 181 gctgttagga ggatgcggcg cgggaacaca ggaagcccag caagtagcag gcgggaatac 241 cactgaatca ggccaggata cgctaaaaaa agccaagagc caggggtttg tcacggtagg 301 cttcgccaat gaaaagccgt acgcctatgc cagtccagat ggaaagctga cgggagaagc 361 ggtcgaggtg gcgcgagtga tcctgaaacg gatgggaatc aatgagatga atggcgtgct 421 taccgagttc ggctccctga ttcccggact gaaggcgaag cgatttgaca tcatcacggc 481 cggtatgtac atcactccaa aacgggcaca agaggttgcg tttgcaaacc ctgaatacag 541 catcggggag gctctcgcag tgaagaaggg caatccgctc ggtcttaaaa gctacaaaga 601 cattgcggct aagccgcagg caaaagtagc ggtgatgagc ggcggcatcg aaacggagta 661 tctcgaaaaa tcgggagtga aaaaggaaca gatggtgctt gttcctgatc aaccttctgc 721 catagccgct ttgcaggcag gaagagcgga tgccataacg atgacaggtc catccctgca 781 agccatgctt gagagcgcca aagacgacca gctggagcgt gtgatggatt ttgtccaacc 841 ggaaattgaa gggaagagcg tacgcggtta tggggcagcg gccttccgca aagaggatga 901 agcgttcgtg aaagcctaca atgaggagtt gcaaaagctg aaagaatcag gggaactgtt 961 ggaaattttg aagcctttcg gttttactga gcaggagctg ccgggtgata tgacagcgaa 1021 tgagcttttg cagcagtagt ggagggagaa gctatgagtg atcttctgcc gttattggca 1081 aaaggagctg gaaccactct ccaggtgaca gtcttgtcgg tgcttgtcgc tctgtttgtt 1141 gcatttctgg cagggtttgg acgtctcagc cgatggagcg gcattcgtat tgtgagtacg 1201 gtttacgtag agttatttcg cgggacctct cttttagtgc aattgttctg gttattttac 1261 gctttgccgc tgttggagat cgaactaccg cctctggctg cgggagtcat tgcgctggga 1321 ctcaactacg gcgcttatgg atcagaggtt gtgcgcagct ccattcaggc gattccaaaa 1381 ggacagaccg aagcggcgat tgccttgaat atgacagcat ggcaacgaat gaagctggtc 1441 atcttgccgc aagccctcgt catgatgctt cctgcattcg gaaatctgtt gattgaactt 1501 ttgaagggta catccctgat ttcattgatt actcttgcgg atttgacgtt ccagggcatg 1561 atattgcgta caactgactc gtccagaaca tttgaaatat tctttgtgat tttggttatg 1621 tactttctca tggcgtatcc actgacactt gcgatgcggt ggcttgaaag gcgctttcat 1681 gcagggaggg cgtaagaaaa atgtggagct ggtcttttac gtggagtatc cttcctgaaa 1741 ttttgcaagc gatccaaacg acgattgtgg cgacgctggc cggttttgtc ctagccatgg 1801 tcgggggctt ggtacttgcc ttcgggcgca ggtcgaaaag gcgagtgctc tcctggtctt 1861 ccgcggcatt tatcgaattt gtccgcagca cgccgcttct cgtgcagctg tactttctct 1921 tttacgggct tccggcgatt ggtatctcgc ttggtcccct aacctctggt gttcttggtt 1981 tgggtattca ttacagctgc tatttgtcag aagtataccg atccggcatt gatgctgtcc 2041 cccgcgggca atgggaagca gcacgggcac tgaacttttc catcgggcag acatggctgc 2101 gcattatttt accgcaagcg atccgtccag tggtccccgt gctgggcaac tatttgatcg 2161 tcatgttcaa ggagacgccc atcctgtcag cgattacctt agtggaaatg ctggcgacag 2221 ccaagatcat cggtgccgaa tcgttccgtt atctggaggc gttcacggtg gtgggacttc 2281 tgtttctggt gcttagctac ggttcctccc ttttgatcaa aaaatgggag cagtcttgga 2341 ataaacagta catgcagagg tgatcgacat gaaaacaggc atacagacgg aacccgtcgt 2401 tcgttaccga aatgtgagca aagcctatgg tgacctgcaa attttaaaag ggattgacct 2461 agatattcat cctggggaaa aaattgcggt cattggcccg agcggttccg gtaaaacgac 2521 gctcgcccgt atattgatga cgctggagga gccgacggag ggaacagttg aggtggctgg 2581 tcaaaattta tggcagaagc aagtaagcgg cagactggtc aaggcggatg agaagcattt 2641 gcacagcatc cgtgggaata tcgggatggt tttccagcat tttaatctgt tcccgcatat 2701 gaccatcctg cgaaatgtaa cagaggctcc gatccatgtc ttgggtatga gcaaggagga 2761 ggcaaaagaa cgggccgtga cgatgttgaa aaaagttggg ctcgatgaca agctggctgc 2821 ttatccttcc cagctttctg gagggcagaa gcagcgagtg gcaatcgccc gtgcgctggt 2881 gatgcgtcca aaggtcatgc tgttcgacga agtaacgtcc gcgctcgatc cggagctagt 2941 gggcgaggtg cttgccgtga tcaaggaaat cgccagggaa ggtgagatgg cgatgctgtt 3001 gatcacccat gagatggatt ttgctcgcga tgttgcggat cgcgtcattt ttacagacgg 3061 tggcacgatt gtcgagcaag gttctcccga gcaaattttt tacaatccgc aaagcccgcg 3121 tttgcaggcg tttctgagcc gctttcgtgc cggctgatga aaggaggtga aatctttgaa 3181 agcgacaagt cgagaccgca tcgtcctgcg cctgccgact gaggaggatg gagcaagcat 3241 gtgggagctg gcaagagatt caaagacgct tgacctgaat tcttcctatg cctacttgtt 3301 gtttgccaag tatttttcgg acacatgcct tattgcagag gaagaaggca aagctgtcgg 3361 ttttgtaatg ggcttccatc cgccagagtc tccggatact ttgtttgttt ggcagattgg 3421 ggtatccgag agcaagcgcg gtcaaggaat tgccaaagcg atgctccgga caatgcttca 3481 gcgaacagcc aagagtgacg tgcgtttttt ggaggcgaca gtctcccctt caaacggcgc 3541 ttcccgtgcg cttttcacga aaatcgccaa ggagcttgct accgaatgtg aagtaacgga 3601 gtgctttccc gcaagattgt ttccgggacg tacacacgag ccggaatgga cgtaccgaat 3661 cggtcccgtt cccaccattt cataaggagg ttttgcatga aaacgattgt cgagacgaga 3721 tcacatttat ctgtgtttga ccagatggaa tcggaagtga gaagttattg ccgcagcttt 3781 cctgtcgtct tcaccaaggc gagcgggcat acggtctggg atcgtcaggg caaggagtac 3841 atcgactttt ttgcaggtgc gggtgcgctc aattacggac acaacaaccc tgcgatcaag 3901 aaaaagctga tggagtacct gcaacaggac ggaatcacgc acagcctgga tatggcgaca 3961 gaagcaaagg aagcgctgct cacccaattt cacgagagta tactcacgcc aagagggctt 4021 tcctacaaag tgatgtttcc agggccaacc gggaccaact cggtggaagc agcattgaag 4081 ctcgcccgca aagccaccgg gagagagatg atcatgagct ttacgaacgg ctttcacgga 4141 atgacccttg gttctttggc tgccacaggc aaccggatga aaagaaaggg agcaggtgtc 4201 ccgctctccc atgtgatggc tctgcctttt gataagtaca tgggcgaggg aatcgacact 4261 acggtttata ttgaacggct gctcgaagac gatggcagcg gcgtacctct ccccgcagct 4321 atgatcctcg aaaccgttca gggggagggc ggcatccatg tagcgagtgc ctcgtggctg 4381 agacagatcg agcgtatctg ccgcaaacac gacatcctgt taatcgtgga tgacgtgcaa 4441 gcgggatgcg gacgaacggg tcctttcttc agctttgaag aggcagacat tacacctgac 4501 atcgtgtgcc tgtccaaatc gattggggga ttcggcttgc ccttttcgct tacactcctg 4561 aagccggagc ttgatgtgtg gctgccgggc gagcataacg gaacctttcg gggcaataat 4621 ctcagcttca tcgcggcagc cgaagcgctg gagtactggc gagacgacag cctcagcgag 4681 tcagtcaagc aaaaggggag catcgtgcaa acgcatttgt cacatctggt gcaaacgtat 4741 cctgacctgt cgggagaagt gagagggaag ggcttgatgc aaggaatcgc cgtgggtgtc 4801 gacgggttgg cagaagaaat atgcacgcgg gcatttcaac gaggactgat tatggagaca 4861 tccgggccga aaagcgaagt catgaaagtc atgccgcctt tgaccatcac cgaagaggcg 4921 ctgcgcaaag gcttgaacat gctggatgag gccgtgaagg agtgcctatc tggcgatttt 4981 cgaaaaagga gtgaaggaaa atgatcgtca agcatttgga agaaattatg gggaccaagg 5041 atgacattga tacgccaaca tggagcagca gacggatgct tttgaagaag gatagcatgg 5101 gattttccat gcatgacacg attatcaagg cgggaacgga gaccttcatc tggtacaaaa 5161 accatctgga agccgtctat tgcatagaag gcgagggaga aatcgaggtg gtaggcggcg 5221 agacctacca gatcaagcca ggaatgatgt acgccttgtc ggaaaatgaa aaacactatt 5281 tgcgcgctag aagccagatg aggatggtgt gcgtcttcaa tcctccgctg acaggccgtg 5341 aagtccatga tgaagaaggc gcttatcctt tgctggaagt gtaatgattt ttaaaaggag 5401 aaaggagctg tcgtatgaag caatcaagac ctgaactact gatagaagat caataccctt 5461 cccgcatcca tgagcatccc gcactcctgc aacgagtaga tcctgtcgtc tactggaatg 5521 aaaagacaaa ggaagggccg cttacgcagg agtcgctgca atcgtatgag aacaaaggct 5581 acatcagtct gcgaggattt ttctcgccgg aggaagtgca ggaattgcgc gatgaattga 5641 ccaaactctg gcaagaaaac cgcgggtcca cacgcaaaga ggttatttgc gagccgcata 5701 gcaacgaagt ccgttccatt tttgccgttc accgtgataa cctcgtgttt caacacttgt 5761 ctcgtcatcc gcgcttagtc tctatcgctc aacagatttt ggggagtgag gtttacatcc 5821 atcaatcccg gatcaattac aagcatggct ttacagggaa agaattttac tggcactccg 5881 actttgagac ctggcatgtc gaggacggga tgccgcgcat gagagcactc agctgctcga 5941 tttcactgga agaaaactat cattacaatg ggccgctgct attgctgcca ggctcgcatc 6001 aaacgtttgt ctcatgcgtg ggccagacgc cagagtctca ttacgagcat tccttgcgca 6061 aacaggagta cggtgtacca gatcagggaa gcttgaagaa aatggtggag cagcacggaa 6121 ttgatacgat gatcggtcct gccggttcag tagtcttttt tgactgcaat actatgcacg 6181 gctccaatgg aaatataaca cctatgccgc gaagcaatgt attcatggtc tacaacagcg 6241 tagaaaatcg gttggtagct cctttttccg gccaaaaacc aagaccggat tacatcgcca 6301 caagggacta aggagtacgg ctcacaagtc tagcaagcaa atgggaggag aaaaatggct 6361 ctcgttgtac aaaagtatgg tggcacatcg gttgcttgcg tggaacgaat tcgcgcggtt 6421 gcgcaacgga ttgcccaatc aagacaaagc ggaaacaagg tagtggtagt tttgtcagcg 6481 atggggaaaa ccacggatcg gctgctgaaa atggcgacgg acatcgatcc ttgcccagaa 6541 ggacgtgagc tagacatgct ggtcagtacg ggagagcagg cagctgcggc tcttctatcg 6601 attgctctgc gtcaacaggg ctgcaatgcc atttcacttg ccgggtggca ggctgggatt 6661 ctgactgaag ccaaacacac cgatgcccaa atcgtgcaca ttgatcccac ccccattgaa 6721 agaagattgg cagaaggaaa agtagtagtg gtagcgggtt tccaaggtgt gtcggcagac 6781 ggggaaatca cgacactggg gagaggcggg tcagacacaa gtgcagttgc gctagctgga 6841 agcttaggtg ccgagatttg tgagattttc accgacgtgg aaggtgtata cacggctgac 6901 ccgcgaaccg ttcccactgc tcaaaaaatt caccgcatca cttactcgga tatgctggag 6961 cttgctcgct caggcgctgg tgtccttcac cctcgttctg tggaagaggc gatgaaaaag 7021 caagtaccct tggtcgtgcg ctccagcttt acagagaggg aaggaaccta tgtaggacaa 7081 aatgagagga gctggagcgc acagcaggta tgcggaattg ctcatcagga aaagatgtcc 7141 gttctatgca taaagagaaa atcgtattcg actgaccatg tggataccga gctcactgaa 7201 atacttcatg aagcggacat ttatgcggaa gtatgggcga ggggaaaaaa cagcctcgac 7261 atttgtttgc ccacatacca ggtaaacccc ctgcttgctc tacttcaggt gaaagttgac 7321 ggtggtgtgg atttttggag ggaagagttg gaatggacca aaatcaatat tattggtgtg 7381 aatcactcga gcatacaaga gctgatgcaa aaggctagag acttcacttt tccgattcgc 7441 tgggggagac aaggaacgaa aggggcttct tgtctcgtac ctcaaaaatt cagcaagcgg 7501 gcaattcagg ctttgcatac gggacttggg ctggacgcaa agatgaaggc aatcgttggt 7561 tcatagctga gtatatccaa accgagcgga ggatcgtcca atcggtttgg atatttttta 7621 tctttactgt aggttgtctg aatgagtagt ttgatgtcca tacttgattt ctgaaaaaat 7681 attatcttcg acagttaagt ctcttcgtac cattgggatt ctattctcaa taaaatgaat 7741 taacttctgg atatcttctt tggttgccgg ttgtttatct gatttaagtt gatagcctaa 7801 atttccgctt acttcaatcg tagcccattc aacatcggat atacttgata ttccagcttg 7861 tcgaagtctc atttctaatt tgtcagtgga gagcctgagt ttccgcatgt tttcaataag 7921 caattgtcca ttttcaataa cgaggactgc cttgcctgta ataatggatt cgaataaatt 7981 agatttcaca cccaagtatt caatcgcgat cattccaata acaagaacaa gtgccaagac 8041 aaaagtaggc caatatccgt gaccactaat cggctgaatg agtaacgttc ctatgccaat 8101 catgacaacc acttgggcca tagtcatttg agagatagat tttctgcctg caatacgaag 8161 caaaagagta ccaaatacga taacgccgac ggtttgcata attagtatcc agctcatttt 8221 ttacccccat cttccaatca tgtcagtata ggtttcccaa atgatggagg tattatgaat 8281 gcaaatatgt cttcatctac aatcatgatg tttgcatatc tagttttttg tactttctac 8341 tgttgtctga aattaaattg atagggggat acgtctgtta tgcaattggc agcacatgag 8401 atgcaagatc tacacgactg gcgttaagtt gtgtaaattc cattacgaac atggcctgct 8461 ttcttaatca ggcccaagat acagaattaa actaatcctg caaagccatt tcccattaca 8521 tattcaggac tataacatga aggccgagta tttgggtaaa gccgcgggtc caacgtcaaa 8581 gctacctgtg cctgagatcc agccagtcct gcagtcgttc atacaatccc cggtgccccc 8641 ttctccagtt actcccagaa cgacagttca acaatttaat gaccgggaaa tcgctaccgc 8701 atatttactg acactgaaga gggcaggcag agaatatgcg tgggcaacaa tggagatgag 8761 tcatccggag attagaagct ttttagagga tgcttttcgt atgagttcac atcatgccta 8821 tgacgtctgg taatggatgg taaaaaaggg gttctaccca ttggaacctg cgtcgcaaac 8881 aacgactaac attcttggtg gaatgtatca ggtgttccaa gaacaggtca ccgtacctgt 8941 acaataaagt gtggcatcaa aactgccctc ttcgtttgac agaagagggc agttttccat 9001 atctcaagca tcgaaggttt tggattgcca gtttattgct tataattttc ttgtttccaa 9061 tagtcgttat tcttttttct tggttatttc ccaatgtcca gtacataaca atttgttctt 9121 tggtgcgaat gtaactttcg cgctattcta acaggagttc ttttagccgt gactaaacat 9181 tttacactat ctatggctca taagaagtgg aggaactcga aaatcacctg cgtaataccc 9241 ttcacaatta catatcagcc attcaaaaag ccattggaaa tgctagtgcg cgatggtttt 9301 tcatttgaat ttggttactg ttctcgaaca aaggaacagt gagtcggtat ttcgttgatc 9361 tcatacgcca cacggatggc agattgtatt gctccctgaa tccatccgtg aaaatcagaa 9421 gcatgttcac cagcgaagtg aactcttccc tcaggtgttg caatgtatgg agataaataa 9481 gtttcttgaa atggtttaaa caaggtaaag gctcctgcgg aaaatggatc taacgaccaa 9541 ttatgtgcga ctccagtgac aaatgtcttg tagactacgt caccgtgtat gtcagccatg 9601 atcttgagag ccttcatcac ttgctcttcc tttgacatgc taatccaagg tagcgcatca 9661 tcttcccatg tataactagc taaaacaact ccactttcac ttttaaaatt atgactgggg 9721 tagtaagaaa ggcgaatagg caagtcagta acactttggc caccatacac cccattttct 9781 tcccagaatc tgcgtgaaaa ttggatgcct attttcgttg aagcgacata atgaagctgg 9841 cgaatggcga gccgtttatg ataagaaaac gaatggaagg gttctacttc gacaaattga 9901 agcacagtaa aaggaacggt aatgattgca aggtctgccg ttacttgaaa tgactcaaag 9961 ttttgagtct gttcgccagc gattgtaaca tggttgtcgt actggatgat tttagtcatc 10021 ttacaattgc agtgtatatt ctcttgtaat tgtggtagga atgcatgagg caatttgtca 10081 aacccgcctg atatttcata aaaccgggta gtgggtgtga agaggatgaa gtcgcgcaaa 10141 atttcaagga aagaaagttc gggaaaacct tcaaaaccag acatgacatt gatcatattg 10201 acggcagcag cggacaatga tgggcccaca gggttgttac gcaaaaaaag ttccatcgaa 10261 tatttatcaa actttttgat tatgatgggc caatgccttt cagggtcttg atttataaaa 10321 tctatgaccg gcttgactgc aagttgggct aattcgtcag cagtcttacc tctttcatgc 10381 ggtg //