LOCUS       NZ_VFNO01000010        10384 bp    DNA     linear   CON 16-AUG-2024
DEFINITION  Brevibacillus sp. AG162 Ga0222362_110, whole genome shotgun
            sequence.
ACCESSION   NZ_VFNO01000010
VERSION     NZ_VFNO01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Brevibacillus sp. AG162
  ORGANISM  Brevibacillus sp. AG162
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Brevibacillus.
REFERENCE   1  (bases 1 to 201887)
  AUTHORS   Venturi,V.
  TITLE     Genome sequencing of rice bacterial endophytes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 201887)
  AUTHORS   Venturi,V., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K.,
            Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C.,
            Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUN-2019) Prokaryotic Super Group, DOE Joint Genome
            Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.12.0
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 236.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006715575.1-RS_2024_08_16
            Annotation Date                   :: 08/16/2024 02:19:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,054
            CDSs (total)                      :: 5,923
            Genes (coding)                    :: 5,868
            CDSs (with protein)               :: 5,868
            Genes (RNA)                       :: 131
            rRNAs                             :: 1, 6, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 6, 5 (16S, 23S)
            tRNAs                             :: 111
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 25 of 55
            Pseudo Genes (incomplete)         :: 38 of 55
            Pseudo Genes (internal stop)      :: 3 of 55
            Pseudo Genes (multiple problems)  :: 11 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:50:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 70116
            Orig. end                         :: 80500
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VFNO01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10384
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10384
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10384
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5384](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5384
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            125..1039
                     /gene="ehuB"
                     /locus_tag="FB479_RS24255"
                     /old_locus_tag="FB479_11036"
     CDS             125..1039
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506238.1"
                     /locus_tag="FB479_RS24255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11036"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_255438596.1"
                     /transl_table=11
                     /translation="MTKTSIARWGAMAILTCVLLLGGCGAGTQEAQQVAGGNTTESGQD
                     TLKKAKSQGFVTVGFANEKPYAYASPDGKLTGEAVEVARVILKRMGINEMNGVLTEFGS
                     LIPGLKAKRFDIITAGMYITPKRAQEVAFANPEYSIGEALAVKKGNPLGLKSYKDIAAK
                     PQAKVAVMSGGIETEYLEKSGVKKEQMVLVPDQPSAIAALQAGRADAITMTGPSLQAML
                     ESAKDDQLERVMDFVQPEIEGKSVRGYGAAAFRKEDEAFVKAYNEELQKLKESGELLEI
                     LKPFGFTEQELPGDMTANELLQQ"
     gene            1054..1695
                     /gene="ehuC"
                     /locus_tag="FB479_RS24260"
                     /old_locus_tag="FB479_11037"
     CDS             1054..1695
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754763.1"
                     /locus_tag="FB479_RS24260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11037"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_142066987.1"
                     /transl_table=11
                     /translation="MSDLLPLLAKGAGTTLQVTVLSVLVALFVAFLAGFGRLSRWSGIR
                     IVSTVYVELFRGTSLLVQLFWLFYALPLLEIELPPLAAGVIALGLNYGAYGSEVVRSSI
                     QAIPKGQTEAAIALNMTAWQRMKLVILPQALVMMLPAFGNLLIELLKGTSLISLITLAD
                     LTFQGMILRTTDSSRTFEIFFVILVMYFLMAYPLTLAMRWLERRFHAGRA"
     gene            1701..2363
                     /gene="ehuD"
                     /locus_tag="FB479_RS24265"
                     /old_locus_tag="FB479_11038"
     CDS             1701..2363
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007506236.1"
                     /locus_tag="FB479_RS24265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11038"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_142066988.1"
                     /transl_table=11
                     /translation="MWSWSFTWSILPEILQAIQTTIVATLAGFVLAMVGGLVLAFGRRS
                     KRRVLSWSSAAFIEFVRSTPLLVQLYFLFYGLPAIGISLGPLTSGVLGLGIHYSCYLSE
                     VYRSGIDAVPRGQWEAARALNFSIGQTWLRIILPQAIRPVVPVLGNYLIVMFKETPILS
                     AITLVEMLATAKIIGAESFRYLEAFTVVGLLFLVLSYGSSLLIKKWEQSWNKQYMQR"
     gene            2369..3157
                     /gene="ehuA"
                     /locus_tag="FB479_RS24270"
                     /old_locus_tag="FB479_11039"
     CDS             2369..3157
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transmembrane
                     transport [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuA"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007131784.1"
                     /locus_tag="FB479_RS24270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11039"
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="WP_106655350.1"
                     /transl_table=11
                     /translation="MKTGIQTEPVVRYRNVSKAYGDLQILKGIDLDIHPGEKIAVIGPS
                     GSGKTTLARILMTLEEPTEGTVEVAGQNLWQKQVSGRLVKADEKHLHSIRGNIGMVFQH
                     FNLFPHMTILRNVTEAPIHVLGMSKEEAKERAVTMLKKVGLDDKLAAYPSQLSGGQKQR
                     VAIARALVMRPKVMLFDEVTSALDPELVGEVLAVIKEIAREGEMAMLLITHEMDFARDV
                     ADRVIFTDGGTIVEQGSPEQIFYNPQSPRLQAFLSRFRAG"
     gene            3176..3685
                     /gene="ectA"
                     /locus_tag="FB479_RS24275"
                     /old_locus_tag="FB479_11040"
     CDS             3176..3685
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589527.1"
                     /locus_tag="FB479_RS24275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11040"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_255438597.1"
                     /transl_table=11
                     /translation="MKATSRDRIVLRLPTEEDGASMWELARDSKTLDLNSSYAYLLFAK
                     YFSDTCLIAEEEGKAVGFVMGFHPPESPDTLFVWQIGVSESKRGQGIAKAMLRTMLQRT
                     AKSDVRFLEATVSPSNGASRALFTKIAKELATECEVTECFPARLFPGRTHEPEWTYRIG
                     PVPTIS"
     gene            3697..5004
                     /gene="ectB"
                     /locus_tag="FB479_RS24280"
                     /old_locus_tag="FB479_11041"
     CDS             3697..5004
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (31-365). E-value: 5.2e-83.
                     Score: 270.8. Matches aSDomain:
                     nrpspksdomains_FB479_RS24280_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536117.1"
                     /locus_tag="FB479_RS24280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11041"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_106655351.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-86, bitscore:
                     284.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTIVETRSHLSVFDQMESEVRSYCRSFPVVFTKASGHTVWDRQG
                     KEYIDFFAGAGALNYGHNNPAIKKKLMEYLQQDGITHSLDMATEAKEALLTQFHESILT
                     PRGLSYKVMFPGPTGTNSVEAALKLARKATGREMIMSFTNGFHGMTLGSLAATGNRMKR
                     KGAGVPLSHVMALPFDKYMGEGIDTTVYIERLLEDDGSGVPLPAAMILETVQGEGGIHV
                     ASASWLRQIERICRKHDILLIVDDVQAGCGRTGPFFSFEEADITPDIVCLSKSIGGFGL
                     PFSLTLLKPELDVWLPGEHNGTFRGNNLSFIAAAEALEYWRDDSLSESVKQKGSIVQTH
                     LSHLVQTYPDLSGEVRGKGLMQGIAVGVDGLAEEICTRAFQRGLIMETSGPKSEVMKVM
                     PPLTITEEALRKGLNMLDEAVKECLSGDFRKRSEGK"
     misc_feature    complement(3698..3712)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.91"
                     /tool="antismash"
     aSDomain        3790..4791
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FB479_RS24280_Aminotran_3.1"
                     /evalue="5.20E-83"
                     /label="FB479_RS24280_Aminotran_3.1"
                     /locus_tag="FB479_RS24280"
                     /protein_end="365"
                     /protein_start="31"
                     /score="270.8"
                     /tool="antismash"
                     /translation="FTKASGHTVWDRQGKEYIDFFAGAGALNYGHNNPAIKKKLMEYLQ
                     QDGITHSLDMATEAKEALLTQFHESILTPRGLSYKVMFPGPTGTNSVEAALKLARKATG
                     REMIMSFTNGFHGMTLGSLAATGNRMKRKGAGVPLSHVMALPFDKYMGEGIDTTVYIER
                     LLEDDGSGVPLPAAMILETVQGEGGIHVASASWLRQIERICRKHDILLIVDDVQAGCGR
                     TGPFFSFEEADITPDIVCLSKSIGGFGLPFSLTLLKPELDVWLPGEHNGTFRGNNLSFI
                     AAAEALEYWRDDSLSESVKQKGSIVQTHLSHLVQTYPDLSGEVRGKGLMQGIA"
     gene            5001..5384
                     /locus_tag="FB479_RS24285"
                     /old_locus_tag="FB479_11042"
     CDS             5001..5384
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536118.1"
                     /locus_tag="FB479_RS24285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11042"
                     /product="ectoine synthase"
                     /protein_id="WP_142066989.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.7e-53, bitscore:
                     173.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKHLEEIMGTKDDIDTPTWSSRRMLLKKDSMGFSMHDTIIKAG
                     TETFIWYKNHLEAVYCIEGEGEIEVVGGETYQIKPGMMYALSENEKHYLRARSQMRMVC
                     VFNPPLTGREVHDEEGAYPLLEV"
     misc_feature    complement(5388..5402)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.9"
                     /tool="antismash"
     gene            5415..6311
                     /gene="thpD"
                     /locus_tag="FB479_RS24290"
                     /old_locus_tag="FB479_11043"
     CDS             5415..6311
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010269269.1"
                     /locus_tag="FB479_RS24290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11043"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_142066990.1"
                     /sec_met_domain="PhyH (E-value: 2.7e-34, bitscore: 112.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQSRPELLIEDQYPSRIHEHPALLQRVDPVVYWNEKTKEGPLTQ
                     ESLQSYENKGYISLRGFFSPEEVQELRDELTKLWQENRGSTRKEVICEPHSNEVRSIFA
                     VHRDNLVFQHLSRHPRLVSIAQQILGSEVYIHQSRINYKHGFTGKEFYWHSDFETWHVE
                     DGMPRMRALSCSISLEENYHYNGPLLLLPGSHQTFVSCVGQTPESHYEHSLRKQEYGVP
                     DQGSLKKMVEQHGIDTMIGPAGSVVFFDCNTMHGSNGNITPMPRSNVFMVYNSVENRLV
                     APFSGQKPRPDYIATRD"
     gene            6355..7566
                     /locus_tag="FB479_RS24295"
                     /old_locus_tag="FB479_11044"
     CDS             6355..7566
                     /EC_number="2.7.2.4"
                     /GO_function="GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /locus_tag="FB479_RS24295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11044"
                     /product="aspartate kinase"
                     /protein_id="WP_142066991.1"
                     /transl_table=11
                     /translation="MALVVQKYGGTSVACVERIRAVAQRIAQSRQSGNKVVVVLSAMGK
                     TTDRLLKMATDIDPCPEGRELDMLVSTGEQAAAALLSIALRQQGCNAISLAGWQAGILT
                     EAKHTDAQIVHIDPTPIERRLAEGKVVVVAGFQGVSADGEITTLGRGGSDTSAVALAGS
                     LGAEICEIFTDVEGVYTADPRTVPTAQKIHRITYSDMLELARSGAGVLHPRSVEEAMKK
                     QVPLVVRSSFTEREGTYVGQNERSWSAQQVCGIAHQEKMSVLCIKRKSYSTDHVDTELT
                     EILHEADIYAEVWARGKNSLDICLPTYQVNPLLALLQVKVDGGVDFWREELEWTKINII
                     GVNHSSIQELMQKARDFTFPIRWGRQGTKGASCLVPQKFSKRAIQALHTGLGLDAKMKA
                     IVGS"
     misc_feature    6382..6395
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.82"
                     /tool="antismash"
     gene            complement(7624..8217)
                     /locus_tag="FB479_RS24300"
                     /old_locus_tag="FB479_11045"
     CDS             complement(7624..8217)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016739276.1"
                     /locus_tag="FB479_RS24300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11045"
                     /product="DUF421 domain-containing protein"
                     /protein_id="WP_142066992.1"
                     /transl_table=11
                     /translation="MSWILIMQTVGVIVFGTLLLRIAGRKSISQMTMAQVVVMIGIGTL
                     LIQPISGHGYWPTFVLALVLVIGMIAIEYLGVKSNLFESIITGKAVLVIENGQLLIENM
                     RKLRLSTDKLEMRLRQAGISSISDVEWATIEVSGNLGYQLKSDKQPATKEDIQKLIHFI
                     ENRIPMVRRDLTVEDNIFSEIKYGHQTTHSDNLQ"
     misc_feature    complement(8295..8309)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
     misc_feature    8390..8406
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 22.12"
                     /tool="antismash"
     gene            8537..8833
                     /locus_tag="FB479_RS24305"
                     /old_locus_tag="FB479_11046"
     CDS             8537..8833
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019490.4"
                     /locus_tag="FB479_RS24305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FB479_11046"
                     /product="spore coat protein"
                     /protein_id="WP_255438598.1"
                     /transl_table=11
                     /translation="MKAEYLGKAAGPTSKLPVPEIQPVLQSFIQSPVPPSPVTPRTTVQ
                     QFNDREIATAYLLTLKRAGREYAWATMEMSHPEIRSFLEDAFRMSSHHAYDVW"
     misc_feature    9035..9050
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.82"
                     /tool="antismash"
     misc_feature    complement(10308..10321)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 19.94"
                     /tool="antismash"
ORIGIN
        1 gcatgcgtat ttagagaaat cagtcggcgc attttgcttt tccgtcaggg atggccctcc
       61 tgaagcggaa gcgatgtgcc gacttttttt ctaccatttc tcataaaaga gaggggcaag
      121 aggaatgaca aagactagca tagcgagatg gggggctatg gcgatcctga cgtgtgtctt
      181 gctgttagga ggatgcggcg cgggaacaca ggaagcccag caagtagcag gcgggaatac
      241 cactgaatca ggccaggata cgctaaaaaa agccaagagc caggggtttg tcacggtagg
      301 cttcgccaat gaaaagccgt acgcctatgc cagtccagat ggaaagctga cgggagaagc
      361 ggtcgaggtg gcgcgagtga tcctgaaacg gatgggaatc aatgagatga atggcgtgct
      421 taccgagttc ggctccctga ttcccggact gaaggcgaag cgatttgaca tcatcacggc
      481 cggtatgtac atcactccaa aacgggcaca agaggttgcg tttgcaaacc ctgaatacag
      541 catcggggag gctctcgcag tgaagaaggg caatccgctc ggtcttaaaa gctacaaaga
      601 cattgcggct aagccgcagg caaaagtagc ggtgatgagc ggcggcatcg aaacggagta
      661 tctcgaaaaa tcgggagtga aaaaggaaca gatggtgctt gttcctgatc aaccttctgc
      721 catagccgct ttgcaggcag gaagagcgga tgccataacg atgacaggtc catccctgca
      781 agccatgctt gagagcgcca aagacgacca gctggagcgt gtgatggatt ttgtccaacc
      841 ggaaattgaa gggaagagcg tacgcggtta tggggcagcg gccttccgca aagaggatga
      901 agcgttcgtg aaagcctaca atgaggagtt gcaaaagctg aaagaatcag gggaactgtt
      961 ggaaattttg aagcctttcg gttttactga gcaggagctg ccgggtgata tgacagcgaa
     1021 tgagcttttg cagcagtagt ggagggagaa gctatgagtg atcttctgcc gttattggca
     1081 aaaggagctg gaaccactct ccaggtgaca gtcttgtcgg tgcttgtcgc tctgtttgtt
     1141 gcatttctgg cagggtttgg acgtctcagc cgatggagcg gcattcgtat tgtgagtacg
     1201 gtttacgtag agttatttcg cgggacctct cttttagtgc aattgttctg gttattttac
     1261 gctttgccgc tgttggagat cgaactaccg cctctggctg cgggagtcat tgcgctggga
     1321 ctcaactacg gcgcttatgg atcagaggtt gtgcgcagct ccattcaggc gattccaaaa
     1381 ggacagaccg aagcggcgat tgccttgaat atgacagcat ggcaacgaat gaagctggtc
     1441 atcttgccgc aagccctcgt catgatgctt cctgcattcg gaaatctgtt gattgaactt
     1501 ttgaagggta catccctgat ttcattgatt actcttgcgg atttgacgtt ccagggcatg
     1561 atattgcgta caactgactc gtccagaaca tttgaaatat tctttgtgat tttggttatg
     1621 tactttctca tggcgtatcc actgacactt gcgatgcggt ggcttgaaag gcgctttcat
     1681 gcagggaggg cgtaagaaaa atgtggagct ggtcttttac gtggagtatc cttcctgaaa
     1741 ttttgcaagc gatccaaacg acgattgtgg cgacgctggc cggttttgtc ctagccatgg
     1801 tcgggggctt ggtacttgcc ttcgggcgca ggtcgaaaag gcgagtgctc tcctggtctt
     1861 ccgcggcatt tatcgaattt gtccgcagca cgccgcttct cgtgcagctg tactttctct
     1921 tttacgggct tccggcgatt ggtatctcgc ttggtcccct aacctctggt gttcttggtt
     1981 tgggtattca ttacagctgc tatttgtcag aagtataccg atccggcatt gatgctgtcc
     2041 cccgcgggca atgggaagca gcacgggcac tgaacttttc catcgggcag acatggctgc
     2101 gcattatttt accgcaagcg atccgtccag tggtccccgt gctgggcaac tatttgatcg
     2161 tcatgttcaa ggagacgccc atcctgtcag cgattacctt agtggaaatg ctggcgacag
     2221 ccaagatcat cggtgccgaa tcgttccgtt atctggaggc gttcacggtg gtgggacttc
     2281 tgtttctggt gcttagctac ggttcctccc ttttgatcaa aaaatgggag cagtcttgga
     2341 ataaacagta catgcagagg tgatcgacat gaaaacaggc atacagacgg aacccgtcgt
     2401 tcgttaccga aatgtgagca aagcctatgg tgacctgcaa attttaaaag ggattgacct
     2461 agatattcat cctggggaaa aaattgcggt cattggcccg agcggttccg gtaaaacgac
     2521 gctcgcccgt atattgatga cgctggagga gccgacggag ggaacagttg aggtggctgg
     2581 tcaaaattta tggcagaagc aagtaagcgg cagactggtc aaggcggatg agaagcattt
     2641 gcacagcatc cgtgggaata tcgggatggt tttccagcat tttaatctgt tcccgcatat
     2701 gaccatcctg cgaaatgtaa cagaggctcc gatccatgtc ttgggtatga gcaaggagga
     2761 ggcaaaagaa cgggccgtga cgatgttgaa aaaagttggg ctcgatgaca agctggctgc
     2821 ttatccttcc cagctttctg gagggcagaa gcagcgagtg gcaatcgccc gtgcgctggt
     2881 gatgcgtcca aaggtcatgc tgttcgacga agtaacgtcc gcgctcgatc cggagctagt
     2941 gggcgaggtg cttgccgtga tcaaggaaat cgccagggaa ggtgagatgg cgatgctgtt
     3001 gatcacccat gagatggatt ttgctcgcga tgttgcggat cgcgtcattt ttacagacgg
     3061 tggcacgatt gtcgagcaag gttctcccga gcaaattttt tacaatccgc aaagcccgcg
     3121 tttgcaggcg tttctgagcc gctttcgtgc cggctgatga aaggaggtga aatctttgaa
     3181 agcgacaagt cgagaccgca tcgtcctgcg cctgccgact gaggaggatg gagcaagcat
     3241 gtgggagctg gcaagagatt caaagacgct tgacctgaat tcttcctatg cctacttgtt
     3301 gtttgccaag tatttttcgg acacatgcct tattgcagag gaagaaggca aagctgtcgg
     3361 ttttgtaatg ggcttccatc cgccagagtc tccggatact ttgtttgttt ggcagattgg
     3421 ggtatccgag agcaagcgcg gtcaaggaat tgccaaagcg atgctccgga caatgcttca
     3481 gcgaacagcc aagagtgacg tgcgtttttt ggaggcgaca gtctcccctt caaacggcgc
     3541 ttcccgtgcg cttttcacga aaatcgccaa ggagcttgct accgaatgtg aagtaacgga
     3601 gtgctttccc gcaagattgt ttccgggacg tacacacgag ccggaatgga cgtaccgaat
     3661 cggtcccgtt cccaccattt cataaggagg ttttgcatga aaacgattgt cgagacgaga
     3721 tcacatttat ctgtgtttga ccagatggaa tcggaagtga gaagttattg ccgcagcttt
     3781 cctgtcgtct tcaccaaggc gagcgggcat acggtctggg atcgtcaggg caaggagtac
     3841 atcgactttt ttgcaggtgc gggtgcgctc aattacggac acaacaaccc tgcgatcaag
     3901 aaaaagctga tggagtacct gcaacaggac ggaatcacgc acagcctgga tatggcgaca
     3961 gaagcaaagg aagcgctgct cacccaattt cacgagagta tactcacgcc aagagggctt
     4021 tcctacaaag tgatgtttcc agggccaacc gggaccaact cggtggaagc agcattgaag
     4081 ctcgcccgca aagccaccgg gagagagatg atcatgagct ttacgaacgg ctttcacgga
     4141 atgacccttg gttctttggc tgccacaggc aaccggatga aaagaaaggg agcaggtgtc
     4201 ccgctctccc atgtgatggc tctgcctttt gataagtaca tgggcgaggg aatcgacact
     4261 acggtttata ttgaacggct gctcgaagac gatggcagcg gcgtacctct ccccgcagct
     4321 atgatcctcg aaaccgttca gggggagggc ggcatccatg tagcgagtgc ctcgtggctg
     4381 agacagatcg agcgtatctg ccgcaaacac gacatcctgt taatcgtgga tgacgtgcaa
     4441 gcgggatgcg gacgaacggg tcctttcttc agctttgaag aggcagacat tacacctgac
     4501 atcgtgtgcc tgtccaaatc gattggggga ttcggcttgc ccttttcgct tacactcctg
     4561 aagccggagc ttgatgtgtg gctgccgggc gagcataacg gaacctttcg gggcaataat
     4621 ctcagcttca tcgcggcagc cgaagcgctg gagtactggc gagacgacag cctcagcgag
     4681 tcagtcaagc aaaaggggag catcgtgcaa acgcatttgt cacatctggt gcaaacgtat
     4741 cctgacctgt cgggagaagt gagagggaag ggcttgatgc aaggaatcgc cgtgggtgtc
     4801 gacgggttgg cagaagaaat atgcacgcgg gcatttcaac gaggactgat tatggagaca
     4861 tccgggccga aaagcgaagt catgaaagtc atgccgcctt tgaccatcac cgaagaggcg
     4921 ctgcgcaaag gcttgaacat gctggatgag gccgtgaagg agtgcctatc tggcgatttt
     4981 cgaaaaagga gtgaaggaaa atgatcgtca agcatttgga agaaattatg gggaccaagg
     5041 atgacattga tacgccaaca tggagcagca gacggatgct tttgaagaag gatagcatgg
     5101 gattttccat gcatgacacg attatcaagg cgggaacgga gaccttcatc tggtacaaaa
     5161 accatctgga agccgtctat tgcatagaag gcgagggaga aatcgaggtg gtaggcggcg
     5221 agacctacca gatcaagcca ggaatgatgt acgccttgtc ggaaaatgaa aaacactatt
     5281 tgcgcgctag aagccagatg aggatggtgt gcgtcttcaa tcctccgctg acaggccgtg
     5341 aagtccatga tgaagaaggc gcttatcctt tgctggaagt gtaatgattt ttaaaaggag
     5401 aaaggagctg tcgtatgaag caatcaagac ctgaactact gatagaagat caataccctt
     5461 cccgcatcca tgagcatccc gcactcctgc aacgagtaga tcctgtcgtc tactggaatg
     5521 aaaagacaaa ggaagggccg cttacgcagg agtcgctgca atcgtatgag aacaaaggct
     5581 acatcagtct gcgaggattt ttctcgccgg aggaagtgca ggaattgcgc gatgaattga
     5641 ccaaactctg gcaagaaaac cgcgggtcca cacgcaaaga ggttatttgc gagccgcata
     5701 gcaacgaagt ccgttccatt tttgccgttc accgtgataa cctcgtgttt caacacttgt
     5761 ctcgtcatcc gcgcttagtc tctatcgctc aacagatttt ggggagtgag gtttacatcc
     5821 atcaatcccg gatcaattac aagcatggct ttacagggaa agaattttac tggcactccg
     5881 actttgagac ctggcatgtc gaggacggga tgccgcgcat gagagcactc agctgctcga
     5941 tttcactgga agaaaactat cattacaatg ggccgctgct attgctgcca ggctcgcatc
     6001 aaacgtttgt ctcatgcgtg ggccagacgc cagagtctca ttacgagcat tccttgcgca
     6061 aacaggagta cggtgtacca gatcagggaa gcttgaagaa aatggtggag cagcacggaa
     6121 ttgatacgat gatcggtcct gccggttcag tagtcttttt tgactgcaat actatgcacg
     6181 gctccaatgg aaatataaca cctatgccgc gaagcaatgt attcatggtc tacaacagcg
     6241 tagaaaatcg gttggtagct cctttttccg gccaaaaacc aagaccggat tacatcgcca
     6301 caagggacta aggagtacgg ctcacaagtc tagcaagcaa atgggaggag aaaaatggct
     6361 ctcgttgtac aaaagtatgg tggcacatcg gttgcttgcg tggaacgaat tcgcgcggtt
     6421 gcgcaacgga ttgcccaatc aagacaaagc ggaaacaagg tagtggtagt tttgtcagcg
     6481 atggggaaaa ccacggatcg gctgctgaaa atggcgacgg acatcgatcc ttgcccagaa
     6541 ggacgtgagc tagacatgct ggtcagtacg ggagagcagg cagctgcggc tcttctatcg
     6601 attgctctgc gtcaacaggg ctgcaatgcc atttcacttg ccgggtggca ggctgggatt
     6661 ctgactgaag ccaaacacac cgatgcccaa atcgtgcaca ttgatcccac ccccattgaa
     6721 agaagattgg cagaaggaaa agtagtagtg gtagcgggtt tccaaggtgt gtcggcagac
     6781 ggggaaatca cgacactggg gagaggcggg tcagacacaa gtgcagttgc gctagctgga
     6841 agcttaggtg ccgagatttg tgagattttc accgacgtgg aaggtgtata cacggctgac
     6901 ccgcgaaccg ttcccactgc tcaaaaaatt caccgcatca cttactcgga tatgctggag
     6961 cttgctcgct caggcgctgg tgtccttcac cctcgttctg tggaagaggc gatgaaaaag
     7021 caagtaccct tggtcgtgcg ctccagcttt acagagaggg aaggaaccta tgtaggacaa
     7081 aatgagagga gctggagcgc acagcaggta tgcggaattg ctcatcagga aaagatgtcc
     7141 gttctatgca taaagagaaa atcgtattcg actgaccatg tggataccga gctcactgaa
     7201 atacttcatg aagcggacat ttatgcggaa gtatgggcga ggggaaaaaa cagcctcgac
     7261 atttgtttgc ccacatacca ggtaaacccc ctgcttgctc tacttcaggt gaaagttgac
     7321 ggtggtgtgg atttttggag ggaagagttg gaatggacca aaatcaatat tattggtgtg
     7381 aatcactcga gcatacaaga gctgatgcaa aaggctagag acttcacttt tccgattcgc
     7441 tgggggagac aaggaacgaa aggggcttct tgtctcgtac ctcaaaaatt cagcaagcgg
     7501 gcaattcagg ctttgcatac gggacttggg ctggacgcaa agatgaaggc aatcgttggt
     7561 tcatagctga gtatatccaa accgagcgga ggatcgtcca atcggtttgg atatttttta
     7621 tctttactgt aggttgtctg aatgagtagt ttgatgtcca tacttgattt ctgaaaaaat
     7681 attatcttcg acagttaagt ctcttcgtac cattgggatt ctattctcaa taaaatgaat
     7741 taacttctgg atatcttctt tggttgccgg ttgtttatct gatttaagtt gatagcctaa
     7801 atttccgctt acttcaatcg tagcccattc aacatcggat atacttgata ttccagcttg
     7861 tcgaagtctc atttctaatt tgtcagtgga gagcctgagt ttccgcatgt tttcaataag
     7921 caattgtcca ttttcaataa cgaggactgc cttgcctgta ataatggatt cgaataaatt
     7981 agatttcaca cccaagtatt caatcgcgat cattccaata acaagaacaa gtgccaagac
     8041 aaaagtaggc caatatccgt gaccactaat cggctgaatg agtaacgttc ctatgccaat
     8101 catgacaacc acttgggcca tagtcatttg agagatagat tttctgcctg caatacgaag
     8161 caaaagagta ccaaatacga taacgccgac ggtttgcata attagtatcc agctcatttt
     8221 ttacccccat cttccaatca tgtcagtata ggtttcccaa atgatggagg tattatgaat
     8281 gcaaatatgt cttcatctac aatcatgatg tttgcatatc tagttttttg tactttctac
     8341 tgttgtctga aattaaattg atagggggat acgtctgtta tgcaattggc agcacatgag
     8401 atgcaagatc tacacgactg gcgttaagtt gtgtaaattc cattacgaac atggcctgct
     8461 ttcttaatca ggcccaagat acagaattaa actaatcctg caaagccatt tcccattaca
     8521 tattcaggac tataacatga aggccgagta tttgggtaaa gccgcgggtc caacgtcaaa
     8581 gctacctgtg cctgagatcc agccagtcct gcagtcgttc atacaatccc cggtgccccc
     8641 ttctccagtt actcccagaa cgacagttca acaatttaat gaccgggaaa tcgctaccgc
     8701 atatttactg acactgaaga gggcaggcag agaatatgcg tgggcaacaa tggagatgag
     8761 tcatccggag attagaagct ttttagagga tgcttttcgt atgagttcac atcatgccta
     8821 tgacgtctgg taatggatgg taaaaaaggg gttctaccca ttggaacctg cgtcgcaaac
     8881 aacgactaac attcttggtg gaatgtatca ggtgttccaa gaacaggtca ccgtacctgt
     8941 acaataaagt gtggcatcaa aactgccctc ttcgtttgac agaagagggc agttttccat
     9001 atctcaagca tcgaaggttt tggattgcca gtttattgct tataattttc ttgtttccaa
     9061 tagtcgttat tcttttttct tggttatttc ccaatgtcca gtacataaca atttgttctt
     9121 tggtgcgaat gtaactttcg cgctattcta acaggagttc ttttagccgt gactaaacat
     9181 tttacactat ctatggctca taagaagtgg aggaactcga aaatcacctg cgtaataccc
     9241 ttcacaatta catatcagcc attcaaaaag ccattggaaa tgctagtgcg cgatggtttt
     9301 tcatttgaat ttggttactg ttctcgaaca aaggaacagt gagtcggtat ttcgttgatc
     9361 tcatacgcca cacggatggc agattgtatt gctccctgaa tccatccgtg aaaatcagaa
     9421 gcatgttcac cagcgaagtg aactcttccc tcaggtgttg caatgtatgg agataaataa
     9481 gtttcttgaa atggtttaaa caaggtaaag gctcctgcgg aaaatggatc taacgaccaa
     9541 ttatgtgcga ctccagtgac aaatgtcttg tagactacgt caccgtgtat gtcagccatg
     9601 atcttgagag ccttcatcac ttgctcttcc tttgacatgc taatccaagg tagcgcatca
     9661 tcttcccatg tataactagc taaaacaact ccactttcac ttttaaaatt atgactgggg
     9721 tagtaagaaa ggcgaatagg caagtcagta acactttggc caccatacac cccattttct
     9781 tcccagaatc tgcgtgaaaa ttggatgcct attttcgttg aagcgacata atgaagctgg
     9841 cgaatggcga gccgtttatg ataagaaaac gaatggaagg gttctacttc gacaaattga
     9901 agcacagtaa aaggaacggt aatgattgca aggtctgccg ttacttgaaa tgactcaaag
     9961 ttttgagtct gttcgccagc gattgtaaca tggttgtcgt actggatgat tttagtcatc
    10021 ttacaattgc agtgtatatt ctcttgtaat tgtggtagga atgcatgagg caatttgtca
    10081 aacccgcctg atatttcata aaaccgggta gtgggtgtga agaggatgaa gtcgcgcaaa
    10141 atttcaagga aagaaagttc gggaaaacct tcaaaaccag acatgacatt gatcatattg
    10201 acggcagcag cggacaatga tgggcccaca gggttgttac gcaaaaaaag ttccatcgaa
    10261 tatttatcaa actttttgat tatgatgggc caatgccttt cagggtcttg atttataaaa
    10321 tctatgaccg gcttgactgc aagttgggct aattcgtcag cagtcttacc tctttcatgc
    10381 ggtg
//
