LOCUS NZ_VFNO01000005 10849 bp DNA linear CON 16-AUG-2024 DEFINITION Brevibacillus sp. AG162 Ga0222362_105, whole genome shotgun sequence. ACCESSION NZ_VFNO01000005 VERSION NZ_VFNO01000005.1 KEYWORDS WGS; RefSeq. SOURCE Brevibacillus sp. AG162 ORGANISM Brevibacillus sp. AG162 Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus. REFERENCE 1 (bases 1 to 518238) AUTHORS Venturi,V. TITLE Genome sequencing of rice bacterial endophytes JOURNAL Unpublished REFERENCE 2 (bases 1 to 518238) AUTHORS Venturi,V., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K., Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C., Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T. TITLE Direct Submission JOURNAL Submitted (14-JUN-2019) Prokaryotic Super Group, DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12.0 Genome Representation :: Full Expected Final Version :: No Genome Coverage :: 236.0x Sequencing Technology :: Illumina HiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_006715575.1-RS_2024_08_16 Annotation Date :: 08/16/2024 02:19:50 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.8 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 6,054 CDSs (total) :: 5,923 Genes (coding) :: 5,868 CDSs (with protein) :: 5,868 Genes (RNA) :: 131 rRNAs :: 1, 6, 5 (5S, 16S, 23S) complete rRNAs :: 1 (5S) partial rRNAs :: 6, 5 (16S, 23S) tRNAs :: 111 ncRNAs :: 8 Pseudo Genes (total) :: 55 CDSs (without protein) :: 55 Pseudo Genes (ambiguous residues) :: 0 of 55 Pseudo Genes (frameshifted) :: 25 of 55 Pseudo Genes (incomplete) :: 38 of 55 Pseudo Genes (internal stop) :: 3 of 55 Pseudo Genes (multiple problems) :: 11 of 55 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 15:50:39 NOTE :: This is a single region extracted from a larger record! Orig. start :: 62297 Orig. end :: 73146 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to VFNO01000005.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ FEATURES Location/Qualifiers region 1..10849 /candidate_cluster_numbers="1" /contig_edge="False" /product="RiPP-like" /region_number="1" /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /tool="antismash" cand_cluster 1..10849 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /kind="single" /product="RiPP-like" /protoclusters="1" /tool="antismash" protocluster 1..10849 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="False" /core_location="[5000:5849](+)" /cutoff="20000" /detection_rule="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /neighbourhood="5000" /product="RiPP-like" /protocluster_number="1" /tool="antismash" proto_core 5001..5849 /aStool="rule-based-clusters" /tool="antismash" /cutoff="20000" /detection_rule="(strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))" /neighbourhood="5000" /product="RiPP-like" /protocluster_number="1" gene complement(29..1123) /locus_tag="FB479_RS16585" /old_locus_tag="FB479_10563" CDS complement(29..1123) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015892420.1" /locus_tag="FB479_RS16585" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10563" /product="YheC/YheD family protein" /protein_id="WP_142066027.1" /transl_table=11 /translation="MLPKRVIGILTWREGVRFEEPDYLRKLVQTGQKLGAQIYLFSHQD VNVPARKIKGFIPKPNGGWMSKSFPWPEVVIDRYRRRVKEYIRLRNSDLFFFANSPFSK KWRVTNLLASDERVKRWIPETHVYEKGLVRNMLARHGLVYVKPGNGTGGRSILRVKSSG KQYSLSGRDKKHNHRVARIDTVEGVERWVKTWVEEQRIRDGNFLVQQGLDLGLLRKHVV DVRVLIQKDERGEWVVTGCAVRMGGNGSATSNLHGGGRAIPFEWFMFKRFGEERGELIK QECHQLALDVANTLEDYFGRMMEFGLDIGVDVDGRTWLIEVNPKPGREVFRQMGDMALY KKAIARPVQFALYLARNQARFLSL" gene 1325..1645 /locus_tag="FB479_RS16590" /old_locus_tag="FB479_10564" CDS 1325..1645 /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1167: transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016742092.1" /locus_tag="FB479_RS16590" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10564" /product="helix-turn-helix domain-containing protein" /protein_id="WP_017250460.1" /transl_table=11 /translation="MSQDFSGEHMCPKYECAINVLGKRWTGLIIHVLLRGTVRFKDIRE MVPHMSDKMLSERLKELEELEILERKVYPEIPVRIEYELTEKGKDLRPVIDSIHEWGQK WM" gene 1758..3209 /locus_tag="FB479_RS16595" /old_locus_tag="FB479_10565" CDS 1758..3209 /GO_function="GO:0000150 - DNA strand exchange activity [Evidence IEA]" /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_process="GO:0006310 - DNA recombination [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017250461.1" /locus_tag="FB479_RS16595" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10565" /product="recombinase family protein" /protein_id="WP_142066028.1" /transl_table=11 /translation="MRTAIYIRVSTEDQAREGFSIPAQREKLLSYIHSQGWEVHAVYAD EGASAKDTNRPALGQLLQDIRTGEIDVVLVYRLDRLTRSVLDLYQLLQEFDRYAVHFKS CTEVYDTTTAIGRLFITLVAALAQWERENLAERVKLGMSQMARERKRPGGPAPYGYNLV RGTLVVNQREAAGVRSMFERYDRGESPRQIAEWANQSGLRGKNGASWSASAVLRLLKNP VYHGALRWNYADADQQRNDPEEWIIEEATHPAIIDQSCFSRVQERMSARGTGHPRVLSS RYLFSGLLYCSRCGSSMRGKTTTITGKGEKRYTHRYYLCKNKLAGTCDAPAIREDRLEK AIVHELMQYSPESIAAVKEVYQTVLQANSRAPAKETEQQLQIRRKRWEQAYEEGFLTLS ALREKISALELAAKEREQTYSAFAKGQPLDLDALSNWQLIWTHANEKERRLLVCTVIQR VDAEATDAASGQKNRRVCLHLLSFH" gene 3232..3510 /locus_tag="FB479_RS16600" /old_locus_tag="FB479_10566" CDS 3232..3510 /EC_number="3.4.21.-" /GO_function="GO:0004252 - serine-type endopeptidase activity [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007721243.1" /locus_tag="FB479_RS16600" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10566" /product="S1C family serine protease" /protein_id="WP_007721243.1" /transl_table=11 /translation="MATGGSPLGLENSVTAGIISAKNRRLQVAKRMYEEIFQTDAAINP GNSGGPLINLNGEVVGLNAFIIQSSQCLGFAIGIDALKMQLEQYVFK" gene complement(3541..3687) /locus_tag="FB479_RS30540" /old_locus_tag="FB479_10567" CDS complement(3541..3687) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_005828114.1" /locus_tag="FB479_RS30540" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10567" /product="hypothetical protein" /protein_id="WP_017250462.1" /transl_table=11 /translation="MEQNRLSELPIAQLTAAQLQQLKQAEEQINTEGPNVYLIALEKQS PSS" gene complement(3752..4033) /locus_tag="FB479_RS16605" /old_locus_tag="FB479_10568" CDS complement(3752..4033) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015892425.1" /locus_tag="FB479_RS16605" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10568" /product="DUF2325 domain-containing protein" /protein_id="WP_007721245.1" /transl_table=11 /translation="MSSILVIGGDRLGNIIEFLQGQGFKDIHHVTGRKSSQTGVKIPAG IHMILVLTDFVNHNLAKTVKNQAKDRELPIVFCKRSCSAIAKAFDLTA" misc_feature complement(4081..4095) /note="TFBS match to SypG, Regulator of biofilm formation and host colonization, confidence: weak, score: 20.98" /tool="antismash" misc_feature 4095..4110 /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: medium, score: 24.47" /tool="antismash" misc_feature complement(4097..4111) /note="TFBS match to ZuR_variant_1, Zinc-responsive repressor, confidence: medium, score: 22.53" /tool="antismash" misc_feature complement(4098..4113) /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: medium, score: 24.03" /tool="antismash" misc_feature 4117..4132 /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: medium, score: 24.26" /tool="antismash" misc_feature 4120..4133 /note="TFBS match to PerR, Peroxide regulator, confidence: weak, score: 21.73" /tool="antismash" misc_feature complement(4120..4135) /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: medium, score: 25.13" /tool="antismash" misc_feature complement(4171..4184) /note="TFBS match to MogR, Repressor of flagellar motility genes, confidence: weak, score: 20.64" /tool="antismash" misc_feature complement(4177..4186) /note="TFBS match to ToxT, Regulator of MSHA biosynthesis, confidence: strong, score: 18.57" /tool="antismash" gene 4364..4963 /locus_tag="FB479_RS16610" /old_locus_tag="FB479_10569" CDS 4364..4963 /GO_component="GO:0140737 - encapsulin nanocompartment [Evidence IEA]" /GO_function="GO:0140315 - iron ion sequestering activity [Evidence IEA]" /GO_process="GO:0006880 - intracellular sequestering of iron ion [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016742089.1" /locus_tag="FB479_RS16610" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10569" /product="IMEF encapsulin system ferritin-like cargo protein" /protein_id="WP_048034009.1" /transl_table=11 /translation="MQEITQLHAIFDRTRGYINSFMGVIQPIIDAATDEHTRLYYHHIL EEEEQRMGRLQELVPYLEKLSSGKSLDQLSDRDLSQMLSDVNLERFGLHNFREHLELSL YEFKDDETRQLLDGMREKTHTDYLTVKEIMANMSQRFSDAAHPDLTDHDEGHDIHQVDH LKASASAPHGVASVIKHSAPAVSGKKGLTVGSLKGM" gene 5001..5849 /locus_tag="FB479_RS16615" /old_locus_tag="FB479_10570" CDS 5001..5849 /GO_component="GO:0140737 - encapsulin nanocompartment [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) RiPP-like: Linocin_M18" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_005828110.1" /locus_tag="FB479_RS16615" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10570" /product="family 1 encapsulin nanocompartment shell protein" /protein_id="WP_007721249.1" /sec_met_domain="Linocin_M18 (E-value: 1.1e-83, bitscore: 275.0, seeds: 61, tool: rule-based-clusters)" /transl_table=11 /translation="MDKLRKYPDSPLTTEEWNQLDATVVDMARRQLVGRRFIDIYGPLG EGIQTITNDVYEESRFGGLSLRGESLEMTQPSRRVSMTIPILYKDFMLYWRDVAQARTL GMPLDMSAAANAAAGGALMEDDLIFNGAAEFDLPGLMNVKGRLTHLKSDWMESGNAFAD IVEARNKLLKMGHSGPYALVVSPELYSLLHRVHKGTNVLEIEHVRNLVTDGVFQSPTIK GRSGVLVATGRHNLDLAIAEDFDSAFLGDEQMNSLFRVYECVVLRIKRPSAICTLEETE E" misc_feature complement(5905..5917) /note="TFBS match to LuxO, Repressor of luminescence, confidence: weak, score: 20.68" /tool="antismash" misc_feature 5934..5947 /note="TFBS match to LexA_variant_2, Repressor of DNA damage response, confidence: medium, score: 21.74" /tool="antismash" misc_feature complement(5949..5963) /note="TFBS match to CodY, Regulator of stationary phase and virulence in Gram-positive bacteria, confidence: medium, score: 21.87" /tool="antismash" gene 5993..6810 /locus_tag="FB479_RS16620" /pseudo="" CDS 5993..6810 /NRPS_PKS="Domain: Abhydrolase_1 (19-115). E-value: 1.8e-11. Score: 36.1. Matches aSDomain: nrpspksdomains_FB479_RS16620_Abhydrolase_1.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) PF00561" /gene_functions="biosynthetic-additional (rule-based-clusters) Abhydrolase_6" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007721251.1" /locus_tag="FB479_RS16620" /note="frameshifted; Derived by automated computational analysis using gene prediction method: Protein Homology." /product="alpha/beta fold hydrolase" /pseudo="" /sec_met_domain="PF00561 (E-value: 7.2e-08, bitscore: 27.2, seeds: 48, tool: rule-based-clusters)" /sec_met_domain="Abhydrolase_6 (E-value: 3e-08, bitscore: 29.4, seeds: 455, tool: rule-based-clusters)" /transl_table=11 /translation="MRCEVNGVNLYYEEIGSGFPVVMIHGFSLDHRCMTGCLEPIFKKR PGYRRLYIDLPGMGQTENYEHIHTTDDILEVVLAFIDQLIPGEPFFIAGESYGGYLARA VIEKRRDQVRAPFSFARTSFLTKQSAHCQINPS" aSDomain 6050..6337 /aSDomain="Abhydrolase_1" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_FB479_RS16620_Abhydrolase_1.1" /evalue="1.80E-11" /label="FB479_RS16620_Abhydrolase_1.1" /locus_tag="FB479_RS16620" /protein_end="115" /protein_start="19" /score="36.1" /tool="antismash" /translation="PVVMIHGFSLDHRCMTGCLEPIFKKRPGYRRLYIDLPGMGQTENY EHIHTTDDILEVVLAFIDQLIPGEPFFIAGESYGGYLARAVIEKRRDQVRA" gene complement(6866..8074) /locus_tag="FB479_RS16625" /old_locus_tag="FB479_10573" CDS complement(6866..8074) /GO_function="GO:0030170 - pyridoxal phosphate binding [Evidence IEA]" /NRPS_PKS="Domain: Aminotran_1_2 (36-387). E-value: 5.9e-43. Score: 139.3. Matches aSDomain: nrpspksdomains_FB479_RS16625_Aminotran_1_2.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Aminotran_1_2" /gene_functions="biosynthetic-additional (smcogs) SMCOG1019: aminotransferase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016742086.1" /locus_tag="FB479_RS16625" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10573" /product="PLP-dependent aminotransferase family protein" /protein_id="WP_142066029.1" /sec_met_domain="Aminotran_1_2 (E-value: 3.9e-42, bitscore: 139.5, seeds: 48, tool: rule-based-clusters)" /transl_table=11 /translation="MEYSFSKSVEALSSSAVREILKLTQGNQVLSLAGGLPAEDSFPIA EMREAFNRAFEQGAKALQYGLTDGYLPLREWIASRMKQKHMNVGVDNMVITTGSQQAVD LLCRVYLDEGDVVLVENPTYLAAVQLFQFRGIKAIPVDGDADGMDLDDLAKKIAQYRPK MVYVIPTFANPTGKVWSLERRLGLLRQCKAQNILILEDDPYGEIQFDGEAPYRSIFSLD EHPTDSCVVYTSTFSKIVAPGLRTGWAIGDTRVIQMMVKAKQAVDLQSSTIDQYALYEL LSRFDLERHIEKIRESYKARMEWMHELLVAQNWEGVEWEKPRGGMFLWVNLPEHVDAEK LLARSVQEGVAFVPGKPFYAAEPQVNTLRLNYTLLNREDTELAISRLGKAFHAYSESLV TNV" aSDomain complement(6914..7966) /aSDomain="Aminotran_1_2" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_FB479_RS16625_Aminotran_1_2.1" /evalue="5.90E-43" /label="FB479_RS16625_Aminotran_1_2.1" /locus_tag="FB479_RS16625" /protein_end="387" /protein_start="36" /score="139.3" /tool="antismash" /translation="PAEDSFPIAEMREAFNRAFEQGAKALQYGLTDGYLPLREWIASRM KQKHMNVGVDNMVITTGSQQAVDLLCRVYLDEGDVVLVENPTYLAAVQLFQFRGIKAIP VDGDADGMDLDDLAKKIAQYRPKMVYVIPTFANPTGKVWSLERRLGLLRQCKAQNILIL EDDPYGEIQFDGEAPYRSIFSLDEHPTDSCVVYTSTFSKIVAPGLRTGWAIGDTRVIQM MVKAKQAVDLQSSTIDQYALYELLSRFDLERHIEKIRESYKARMEWMHELLVAQNWEGV EWEKPRGGMFLWVNLPEHVDAEKLLARSVQEGVAFVPGKPFYAAEPQVNTLRLNYTLLN REDTELAISRL" misc_feature complement(8054..8068) /note="TFBS match to SypG, Regulator of biofilm formation and host colonization, confidence: weak, score: 22.38" /tool="antismash" gene complement(8269..8739) /locus_tag="FB479_RS16630" /old_locus_tag="FB479_10574" CDS complement(8269..8739) /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016742085.1" /locus_tag="FB479_RS16630" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10574" /product="hypothetical protein" /protein_id="WP_142066030.1" /transl_table=11 /translation="MRYQINGYTDMYTVIANERKIGGAIEAGQIRLRTGEVYANAVLTR LEMSGAHFCSIGFVTEEGKRLIVHVNDISMIADARHVNVCELTNECMRVEKSAERLKRL KRLCELNEGSCTPTFQEEALLLANDIGMEEASTYVDLSFLPHTEKPRVFRIA" misc_feature complement(8760..8775) /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: medium, score: 24.25" /tool="antismash" misc_feature complement(8789..8804) /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: weak, score: 21.39" /tool="antismash" misc_feature 8792..8807 /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: strong, score: 28.43" /tool="antismash" misc_feature 8794..8808 /note="TFBS match to ZuR_variant_1, Zinc-responsive repressor, confidence: medium, score: 22.75" /tool="antismash" misc_feature 8815..8830 /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: weak, score: 22.88" /tool="antismash" misc_feature 8815..8833 /note="TFBS match to FuR_variant_2, Ferric Uptake Regulator, confidence: weak, score: 22.38" /tool="antismash" misc_feature complement(8817..8831) /note="TFBS match to ZuR_variant_1, Zinc-responsive repressor, confidence: medium, score: 21.27" /tool="antismash" misc_feature complement(8818..8833) /note="TFBS match to FuR_variant_1, Ferric Uptake Regulator, confidence: medium, score: 24.34" /tool="antismash" gene complement(8891..10072) /locus_tag="FB479_RS16635" /old_locus_tag="FB479_10575" CDS complement(8891..10072) /NRPS_PKS="Domain: Aminotran_1_2 (32-383). E-value: 6.6e-63. Score: 204.9. Matches aSDomain: nrpspksdomains_FB479_RS16635_Aminotran_1_2.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Aminotran_1_2" /gene_functions="biosynthetic-additional (smcogs) SMCOG1019: aminotransferase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015892430.1" /locus_tag="FB479_RS16635" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_10575" /product="pyridoxal phosphate-dependent aminotransferase" /protein_id="WP_142066290.1" /sec_met_domain="Aminotran_1_2 (E-value: 4.9e-62, bitscore: 205.0, seeds: 48, tool: rule-based-clusters)" /transl_table=11 /translation="MRIQPSDMIDRLPTQFFATLVAKVNKVIAQGHDVINLGQGNPDLP TPPHIIEELQAQAAQPLHHKYPPFQGRVELKQAVAHWYKQEFDVDLDPEEEVAILFGSK TGLVEICQVLMNAGDVALVPDPGYPDYWSGVAVVNGRMVMMPLKAEKDFLPDYSQLSQA DLDRAKLMFLNYPNNPTAVNAPLEFYEETIRFARKHEIVVCHDFAYGAISYDGKKPVSF MQVPGAKEVGVEFYTLSKTYNMAGWRVGAMVGNRELVRLINLIQDHYFVSLFGAVQMAA AKAMTDSQQCVRDLVAVYESRRNALYSNLHRIGWQAPPSQGSFFAWLPVPSGFTSVEFS DLLLEKAHVVVAPGNGFGPTGEGYVRAALLSNEERLAEAVQRIERLGLFTKTK" aSDomain complement(8924..9976) /aSDomain="Aminotran_1_2" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_FB479_RS16635_Aminotran_1_2.1" /evalue="6.60E-63" /label="FB479_RS16635_Aminotran_1_2.1" /locus_tag="FB479_RS16635" /protein_end="383" /protein_start="32" /score="204.9" /tool="antismash" /translation="DVINLGQGNPDLPTPPHIIEELQAQAAQPLHHKYPPFQGRVELKQ AVAHWYKQEFDVDLDPEEEVAILFGSKTGLVEICQVLMNAGDVALVPDPGYPDYWSGVA VVNGRMVMMPLKAEKDFLPDYSQLSQADLDRAKLMFLNYPNNPTAVNAPLEFYEETIRF ARKHEIVVCHDFAYGAISYDGKKPVSFMQVPGAKEVGVEFYTLSKTYNMAGWRVGAMVG NRELVRLINLIQDHYFVSLFGAVQMAAAKAMTDSQQCVRDLVAVYESRRNALYSNLHRI GWQAPPSQGSFFAWLPVPSGFTSVEFSDLLLEKAHVVVAPGNGFGPTGEGYVRAALLSN EERLAEAVQRI" regulatory complement(10158..10272) /bound_moiety="S-adenosylmethionine" /db_xref="RFAM:RF00162" /inference="COORDINATES: nucleotide motif:Rfam:14.4:RF00162" /inference="COORDINATES: profile:INFERNAL:1.1.5" /note="SAM riboswitch class I; Derived by automated computational analysis using gene prediction method: cmsearch." /regulatory_class="riboswitch" ORIGIN 1 gtcatcagcg tcggttctcc acctgtgttc aaagagacaa aaaacgagcc tggttccggg 61 ccaaatacag cgcaaattga acgggacgag ctatcgcctt tttgtatagc gccatgtcac 121 ccatctggcg aaaaacctct ctccccggct tgggattcac ttcgattaac cacgtacgtc 181 cgtccacatc caccccaatg tccaatccga attccatcat gcgaccgaaa taatcctcga 241 gcgtgtttgc tacatcgagg gcaagctgat gacattcctg tttgatcagc tccccccgct 301 cctctccaaa tcgcttgaac ataaaccatt caaagggaat cgctctgcct cccccgtgca 361 aattggaagt agcactgccg ttccccccca tacgtactgc acagcctgtc actacccatt 421 ctccacgctc gtccttttga atcaataccc gtacatcgac gacatgcttg cgtagcagtc 481 ctaagtccaa accttgctga accaggaaat tgccatcgcg aatgcgctgt tcctccaccc 541 acgttttgac ccatctttcc acgccttcca ccgtatcaat ccgagccacg cgatggttat 601 gctttttgtc acgtccagag aggctatact gcttaccact gctcttcact ctgaggatgc 661 ttcttccccc tgtgccattg cctggcttga catagacgag cccatggcgt gcgagcatgt 721 tgcggaccaa gcctttttcg taaacatgcg tctccggaat ccaacgtttg acgcgttcat 781 cggaagcgag cagattcgtc actctccatt tcttactgaa cggactgttt gcaaagaaaa 841 acagatcgct atttcgcagt cggatatact cctttaccct gcgtcggtac cgatcaatga 901 caacttctgg ccaagggaac gatttactca tccagccccc gttcggtttg gggatgaagc 961 ccttgatctt tcttgcggga acattgacat cctgatgcga aaacaggtag atttgtgcgc 1021 ctaacttttg ccctgtttga accagctttc gcaaataatc tggttcctca aagcgtacac 1081 cctcccgcca ggttaaaatg ccgattaccc gttttggaag catcgcaaca ccctctttgc 1141 ttcttttctt tcgcgattag tgtatgtgtg gacaccttat agggaaacat ttgtccatga 1201 aaacgggctt ggcttctcag ccattgacaa agtgtcattc caacttttcg ttacttactt 1261 ttttcaagct aatctgttag aataaagggc aacaataatg ctgctagata gaggtgacgc 1321 cgatatgagt caagattttt ctggcgaaca catgtgtcca aaatatgaat gtgcaataaa 1381 cgtccttgga aaacgctgga ccggcctgat tattcatgtg ttgctacgtg ggactgtacg 1441 attcaaagat atccgcgaaa tggttccgca tatgagtgat aaaatgctct ctgaaaggct 1501 gaaggagctg gaggagctag agattttgga gcgcaaagtg tacccagaga tcccggttcg 1561 cattgagtac gaattgaccg aaaaaggaaa agatctgcgt cctgtcattg attccattca 1621 cgaatggggc caaaaatgga tgtagcataa ccccacttgt ccaagacgga taagcggggt 1681 tttttatttg cggtcaggca taagctcctc ccttttgctg tacactctag caacccgagg 1741 tgaagggagg acgtgcgatg cgaacggcca tctacattcg agtcagtacg gaggatcagg 1801 cacgcgaagg gttttcgatc cccgctcaaa gggaaaagct gctctcgtac attcactccc 1861 aaggatggga agtccatgca gtctatgcag atgaaggcgc aagtgccaaa gatacgaatc 1921 gccccgcact cggtcagctc ctgcaggata tccgaacggg tgaaatcgat gtcgtcctcg 1981 tctatcgact cgatcggctc actcgctctg ttcttgattt gtatcagctc ctgcaggagt 2041 tcgatcgata tgcggtacac tttaaaagct gcaccgaggt atacgatacg actacggcaa 2101 tcgggcgact ctttataacc ttagtggctg ccctcgcaca atgggaacgg gaaaacttgg 2161 cggaacgggt caagctggga atgagccaga tggcaagaga acgaaaacga ccaggaggac 2221 ctgcaccgta tggatacaat ctggtgcgag gaacgctggt cgtgaatcaa agggaagcag 2281 ctggcgtacg cagcatgttc gagcgctatg atcgaggcga atctccgcgc caaatcgctg 2341 aatgggcgaa tcaatccgga ttgcgcggca aaaatggcgc ttcatggagt gcaagtgccg 2401 tgcttcgcct gttgaaaaat ccggtctatc acggtgcgtt gcgttggaat tacgctgacg 2461 ccgatcaaca acgcaatgac cctgaggaat ggattatcga agaggcgact cacccggcca 2521 tcatcgatca atcatgcttc tcgcgggtcc aggagcggat gagtgctcgc ggaacaggcc 2581 atccccgcgt tctcagctcc cgctacctct tttccggatt gctgtactgc tcgcgttgcg 2641 gctcctcgat gcgcgggaaa acaaccacga tcaccggaaa aggagaaaaa cgttacacgc 2701 accgctacta cttgtgtaaa aacaagctgg ctggtacgtg tgatgctcct gccatacggg 2761 aggatcgcct ggaaaaagcc attgtgcacg agctgatgca gtattcgcca gagtcaatcg 2821 ctgcggtgaa ggaagtgtat cagactgtgt tacaagcgaa ttcccgcgct cccgcgaaag 2881 aaaccgagca acagctgcaa attcgaagga agcgctggga acaggcctat gaagagggat 2941 tccttactct ttctgcctta cgagagaaaa tcagtgcgtt ggaactggct gcaaaagaac 3001 gggaacagac ttactctgct tttgcaaaag gacaaccgct tgatctagat gcgctttcca 3061 attggcagct catttggaca catgccaatg aaaaagaacg gcgattgctc gtatgcacag 3121 tcatccaacg tgttgacgcg gaagcaacag acgctgcctc tggtcagaaa aatcgtaggg 3181 tttgcctgca tctgttgtct tttcactaaa attgcccctg tttttgcctg tatggcgaca 3241 ggtggttccc cactgggttt ggaaaattct gtgacagcgg gaatcatcag tgccaaaaac 3301 cgtcgcttac aggttgcgaa gcgtatgtac gaagaaattt ttcagacgga tgctgccatt 3361 aacccaggaa atagcggagg tccactcatt aatttgaatg gggaagtcgt tggtctcaat 3421 gcattcatca ttcaatccag ccaatgcttg ggcttcgcca tcggaattga tgccctcaaa 3481 atgcaactgg agcaatacgt attcaagtga gaagcatacg attcaacaaa aaaaccccgt 3541 ctaggaggac ggggattgct tctctaatgc aataagatat acatttggac cttctgtgtt 3601 gatttgttct tctgcctgtt ttaattgttg cagctgagcg gccgtaagct gtgcgatagg 3661 cagttcactc aaacgattct gttccattgc gtcattcctc cgttggtgtc agactcacgc 3721 tcgtagtctg cccgatctgg agagtatgta tttacgcagt gagatcaaaa gctttggcaa 3781 tcgcagaaca cgagcgcttg cagaaaacga tgggcagctc gcgatctttg gcttggtttt 3841 tcacagtttt ggccaagttg tgattgacga aatcagtcaa caccaaaatc atgtggatac 3901 cagctggaat tttcaccccg gtttgcgagc ttttccgtcc agttacgtgg tgaatatcct 3961 tgaagccttg accttgcaga aattcgataa tattgcccag acgatctccg ccaattacta 4021 aaatggatga cataacttgt tacctcctta aatttgggtg aatgtaggat ttatgttttg 4081 ttctcagaat gctaattgat aatgaatatc actcttattg ataaatatta tcattcccaa 4141 ttggctttga caatactctc catgataact tttttgtcaa aaaacaaaac tagaaacgaa 4201 gccgataggc aaagccacgg cgctctagcg ttcccgcact cttttccaaa ttacaccgca 4261 tcatattctt cgtaatcata ggaaaatgta cataggtgtt gcccgggtgt tttttccaac 4321 gttataatga aaaaatagat tgtgaatgag gaggtggcgt agggtgcaag aaattacaca 4381 gctgcacgcc atttttgatc gcaccagagg ttatatcaac agcttcatgg gcgttatcca 4441 gccgattatc gatgcggcaa cggacgagca tactcgtctt tactaccatc acattttgga 4501 agaagaagaa cagcgcatgg gtcgcttgca ggaattggtt ccctacctgg agaagctgtc 4561 ctcaggaaaa agcctggatc agctaagtga tcgcgacctc tcgcaaatgc tgtccgatgt 4621 aaatctggaa cgttttggac tgcacaattt ccgcgagcat ctggagctct ctttgtatga 4681 attcaaggat gacgaaacac gtcagctcct ggatggcatg cgagaaaaga cgcatacaga 4741 ctatttgaca gtcaaagaga tcatggcgaa catgagccaa cgattctctg atgcggctca 4801 tcctgatctc actgatcatg atgaggggca cgacattcac caggtcgatc atttgaaagc 4861 ttcggcttcc gctcctcacg gtgttgcttc cgtgatcaag cattccgccc ctgcagtttc 4921 tggcaaaaaa ggactgacag ttggcagctt gaaaggaatg taaagaatcg gaaatcctga 4981 aatgacatgg aggttacact atggacaagc ttcgtaaata cccagactct cccttgacga 5041 ctgaagaatg gaaccagctg gacgcaactg tcgttgacat ggctcgccgc cagcttgtag 5101 gccgtcgttt tatcgatatt tatggaccat tgggtgaagg catccaaacc attaccaacg 5161 atgtatatga ggaatcccgt tttggcggtc tctccctgcg tggagaatca ctggaaatga 5221 cacagcctag ccgccgtgtc agcatgacga ttccgatcct gtacaaggat ttcatgctgt 5281 actggcgcga tgttgcccaa gcacgcacac tgggcatgcc tttggacatg agcgcggctg 5341 ccaacgcagc tgctggtggt gcactgatgg aagatgatct gatcttcaac ggtgctgcgg 5401 aattcgacct cccaggtctg atgaacgtaa aaggccgcct cactcatctg aagagcgatt 5461 ggatggaatc tggcaatgcg tttgcggata tcgtggaagc acgcaacaag ctgttgaaaa 5521 tgggccatag cggtccgtat gcactggttg tttctcctga gctctactct ctcttgcatc 5581 gcgtgcacaa aggcaccaac gtgcttgaga tcgagcatgt tcgcaatctc gttacagatg 5641 gtgtcttcca atctcctacc attaaaggcc gttccggcgt tttggtagcg actggccgcc 5701 acaatctcga cctggcgatt gcagaagact ttgactccgc attcctcgga gacgagcaaa 5761 tgaacagtct gttccgtgtg tatgagtgcg ttgtattgcg gatcaagcgt ccaagcgcaa 5821 tttgcaccct ggaagaaaca gaagaatagg aagctctttg gcacacccat tgacgattct 5881 cgttgtgggt gtgttttttc attcttgaaa tatgcaacag atcacttata ataagaacgt 5941 acgttccaaa ttttcaaaac aatctgacac aaatgtcgtt ggggggcttt ttatgagatg 6001 cgaggtcaat ggcgtcaatc tttattatga ggagattgga agcggctttc ctgtcgtgat 6061 gattcacggt ttttcacttg atcaccgctg catgactggt tgtctggaac caatttttaa 6121 gaaacgtccg ggataccgac gactttacat agacttgccg ggaatgggtc aaacggaaaa 6181 ctacgaacat atccacacta cggacgatat tctggaggtt gtcctcgcgt tcatcgatca 6241 gctcattcca ggtgagccct ttttcattgc aggtgaatca tacggcggct atcttgcgcg 6301 ggcggttatc gaaaagcgcc gagatcaagt aagggcgccc ttttcatttg cccgaacgtc 6361 attcctgaca aagcaaagcg cacactgcca gataaacccg tcctagtaga agagccttcg 6421 ctctgggagc agttgagtga gacagaacga gcggagtttg agtcgatggc ggtagtagct 6481 accaactaca cgtggaaccg ctataaaaaa gagattgtcg acggctacat gctgtcagac 6541 ccttctttcc taagtaaaat cagacaggcg tatagcgtgt ccttcccatt agatactgtc 6601 cccttccccc acccgagtct ttttgttgtg ggcaaacaag atcattctgt cggctatcgc 6661 gatatttggg atatcctcga aggctatccc cgctcttctt ttgccgcact cgattgtgcc 6721 ggacacaact tgcagattga gcagccgctt ctgtttacag agcttgtgaa tgaatggctg 6781 gatcgggcac aaagagagtt ctccctgtaa acacgcataa aaaaagacaa ccagctcaat 6841 ggcaatggtt gtctttttgc ttccattaaa catttgtcac cagagattcg ctgtaggcgt 6901 ggaatgcttt gcccagtcgt gaaatcgcaa gctccgtgtc ttcccggttc aagagtgtat 6961 agttcaagcg cagcgtattg acttgcggtt cggctgcata aaacggcttc cctggcacga 7021 atgccacgcc ttcctgtacg gatcgggcca gcagcttctc agcatcgacg tgctcaggaa 7081 gattcaccca gaggaacatc ccgcctcttg gtttttccca ctctacgccc tcccagtttt 7141 gcgcgaccaa gagctcatgc atccattcca tgcgcgcctt gtaggactcg cggattttct 7201 cgatgtggcg ctccagatca aaccgggata atagctcata cagggcgtat tggtcgattg 7261 tgctcgactg caaatcaacc gcttgcttcg ccttcaccat catttgaatt acgcgggtat 7321 ccccgatggc ccatccggta cgcaagccgg gcgcgacaat tttggaaaag gtactggtgt 7381 ataccacaca ggaatcggtc ggatgctcat ccagtgagaa aatcgagcgg tacggtgctt 7441 ccccatcgaa ttggatctct ccatatgggt cgtcttccag gatgagaatg ttttgcgcct 7501 tacattgacg aagcaaaccg agacggcgtt cgagactcca tacctttcct gtcgggttcg 7561 caaaggtagg aatgacgtac accattttcg ggcgatactg ggctattttt ttcgccagat 7621 cgtccagatc catcccatca gcatcaccgt ctacgggaat tgctttgatt ccccggaatt 7681 ggaacaattg aaccgctgcc aaataggtag ggttttcgac gagaacgaca tcgccttcgt 7741 ctaaatatac acggcacaac aagtctaccg cttgctgaga gcccgtagtg atgaccatat 7801 tatctacacc gacgttcata tgcttttgct tcatgcggga tgcaatccac tcacgcagtg 7861 gcaagtatcc atcagtcaat ccgtattgaa gagcttttgc tccttgttcg aatgcgcggt 7921 tgaacgcttc gcgcatctct gcgattggaa aagaatcctc tgctggcagt ccacctgcga 7981 gcgagagcac ttgattgcct tgggtcagct tcaagatttc ccggacagcc gaggaggata 8041 gtgcttcaac agattttgaa aatgagtact ccatgttccg ttcccccttg attttgttat 8101 ccttgtttta tcactgtcaa cagcgtaagt ccaatccttg ctagctatga gtagataggt 8161 tttgactatg tcgagcggtt gttcaatcgt tagtcttggc acactgcatg caaaaaaccc 8221 ctcttcgtat taaacgaaaa ggggtccata tgtataggct atatcatcct aggcgatgcg 8281 gaacacacgt ggtttctctg tgtgtgggag gaaagagagg tctacatacg tggacgcttc 8341 ttccataccg atatcgtttg ccaacagcaa tgcctcttct tggaaggtcg gcgtacagct 8401 gccttcattc agttcacaga gacgcttcaa tcgcttcagt ctctctgcgg atttttccac 8461 tctcatgcat tcgttcgtca gctcacaaac gttgacgtga cgagcatcag cgatcatgct 8521 gatatcattc acatggacaa tgagtctttt gccttcttct gtaacaaatc cgatcgagca 8581 aaaatgcgca cctgacattt ccaaacgagt gagaacagca ttggcgtaaa cttctccggt 8641 ccgcaagcga atctggcctg cttcaatggc tccgccgatc tttcgctcgt tggcgatcac 8701 cgtatacata tccgtgtaac cgttaatctg atagcgcatg gtaccctccc gctctcgctg 8761 agactaatta tcattaacaa tgccatcgta taatgaaaat cattatctgt caataatgag 8821 aatgattttt attctagaat aaaaaagcca cactccattt gtatggtagt gtggccaaat 8881 tgcctcgcca ttattttgtt ttcgtaaata accccaaccg ctcaatccgc tggacggctt 8941 ccgcgagacg ctcttcattt gataacaaag ccgctcgcac atagccctca cctgtcgggc 9001 caaagccatt tccaggcgct acgacgacat gcgctttttc caaaagcaga tcagaaaatt 9061 cgacggacgt gaagccactt ggaacaggaa gccaagcaaa aaacgagcct tgagatggcg 9121 gagcctgcca gccaatccga tgcaaattgg agtacaaggc attgcggcgg ctttcgtaga 9181 cagccacgag atcgcgaacg cactgctgcg aatccgtcat cgctttggct gccgccatct 9241 gcaccgcgcc gaacagactg acgaagtaat ggtcctgaat gagattgatc agacgaacca 9301 gctcgcgatt tcctaccatc gcaccaacac gccagcctgc catgttgtac gttttggaca 9361 aggtataaaa ttctacaccc acttcttttg cacccggaac ctgcatgaag ctgaccggct 9421 ttttcccgtc atagctgatc gccccgtagg cgaagtcatg gcagacgacg atttcatgtt 9481 ttcgcgcaaa acgaatggtt tcctcataga actcgagtgg tgcgttgact gctgtcggat 9541 tgttcggata gttgagaaac atcagtttcg ctcgatccag gtcagcttgg gagagctggc 9601 tgtagtctgg caagaagtct ttttccgcct tcaacggcat catgaccatc cgtccgttta 9661 caacagcaac ccccgaccaa taatccggat agcccgggtc tggaaccagc gcaacatcgc 9721 ctgcgttcat gagtacttgg caaatctcta ccagtccggt tttgctgccg aacaaaatcg 9781 ctacttcttc ctccggatcg aggtccacat caaactcttg cttataccaa tgagctacag 9841 cctgcttcag ttccacgcgt ccctgaaaag gcggatattt gtgatggagt ggctgtgccg 9901 cttgcgcctg tagttcttca ataatgtgcg gcggtgtcgg caaatcggga ttgccctgcc 9961 ccaggttaat gacgtcatgg ccttgcgcaa tgaccttgtt tacttttgcc accagggtcg 10021 caaaaaactg ggtagggagt cgatcaatca tgtcggaggg ttgaatgcgc atttctttct 10081 tccattcctt tcttgggccc aatacagtaa aacccccttt ttccatacag aaaaaggggg 10141 agatcagctt ggtcacgtcc ttatctgtca ggaaaaaatc ctgctggaat tggcacagca 10201 tctgtccatt acatggaaca gatccgctgc cgggcttcat agggccagtc cctccacctc 10261 tctgaataag tcaaacacac ggaatatact gttttttcgt ctatatcata ctacttgagc 10321 acgtaaaccg tgtcaagatg cttgattctg taggggtacc tgatgtcgcc ttttttgaaa 10381 agaagtgtgg aaggcaaaga atgccacaga caacagacac aagcctgcca tcacgaacaa 10441 taatccctgc gcatgaatcg attgaaaaat gaatgcgcca agcagtggtc cgagcatccg 10501 cccagctgat ccagttcctg caatcagccc ttgaatgaag ccctctcgtc cctctggcgt 10561 aaactgtgca gctactgccg gcaccgctgg ccacacgagc atttctccca gcgtcatgat 10621 gaacatcccc gccacaaagc ctgcataaac cgttgattga gacaaaatca acatcgacaa 10681 ggcaaaaata taagcgccca agagcatttg tgcctttagc gtacgggcaa agcgtcgaat 10741 gacccacgca atcaatggct gaccaaacag gatcaaaccg ccgttcagcg tccacaacaa 10801 actgtattga cggagtgaaa tgcccaaaga ttgcatgtag gtcgacagt //