LOCUS NZ_VFNO01000003 23571 bp DNA linear CON 16-AUG-2024 DEFINITION Brevibacillus sp. AG162 Ga0222362_103, whole genome shotgun sequence. ACCESSION NZ_VFNO01000003 VERSION NZ_VFNO01000003.1 KEYWORDS WGS; RefSeq. SOURCE Brevibacillus sp. AG162 ORGANISM Brevibacillus sp. AG162 Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Brevibacillus. REFERENCE 1 (bases 1 to 910716) AUTHORS Venturi,V. TITLE Genome sequencing of rice bacterial endophytes JOURNAL Unpublished REFERENCE 2 (bases 1 to 910716) AUTHORS Venturi,V., Huntemann,M., Clum,A., Pillay,M., Palaniappan,K., Varghese,N., Mikhailova,N., Stamatis,D., Reddy,T., Daum,C., Shapiro,N., Ivanova,N., Kyrpides,N. and Woyke,T. TITLE Direct Submission JOURNAL Submitted (14-JUN-2019) Prokaryotic Super Group, DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA COMMENT ##Genome-Assembly-Data-START## Assembly Method :: SPAdes v. 3.12.0 Genome Representation :: Full Expected Final Version :: No Genome Coverage :: 236.0x Sequencing Technology :: Illumina HiSeq ##Genome-Assembly-Data-END## ##Genome-Annotation-Data-START## Annotation Provider :: NCBI RefSeq Annotation Name :: GCF_006715575.1-RS_2024_08_16 Annotation Date :: 08/16/2024 02:19:50 Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method :: Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision :: 6.8 Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA Genes (total) :: 6,054 CDSs (total) :: 5,923 Genes (coding) :: 5,868 CDSs (with protein) :: 5,868 Genes (RNA) :: 131 rRNAs :: 1, 6, 5 (5S, 16S, 23S) complete rRNAs :: 1 (5S) partial rRNAs :: 6, 5 (16S, 23S) tRNAs :: 111 ncRNAs :: 8 Pseudo Genes (total) :: 55 CDSs (without protein) :: 55 Pseudo Genes (ambiguous residues) :: 0 of 55 Pseudo Genes (frameshifted) :: 25 of 55 Pseudo Genes (incomplete) :: 38 of 55 Pseudo Genes (internal stop) :: 3 of 55 Pseudo Genes (multiple problems) :: 11 of 55 ##Genome-Annotation-Data-END## ##antiSMASH-Data-START## Version :: 8.dev-cf2fc5ee(changed) Run date :: 2025-09-12 15:50:39 NOTE :: This is a single region extracted from a larger record! Orig. start :: 602014 Orig. end :: 625585 ##antiSMASH-Data-END## REFSEQ INFORMATION: The reference sequence is identical to VFNO01000003.1. The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/ FEATURES Location/Qualifiers region 1..23571 /candidate_cluster_numbers="1" /contig_edge="False" /product="azole-containing-RiPP" /region_number="2" /rules="((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)" /tool="antismash" cand_cluster 1..23571 /candidate_cluster_number="1" /contig_edge="False" /detection_rules="((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)" /kind="single" /product="azole-containing-RiPP" /protoclusters="1" /tool="antismash" protocluster 1..23571 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="False" /core_location="[10000:13571](+)" /cutoff="10000" /detection_rule="((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)" /neighbourhood="10000" /product="azole-containing-RiPP" /protocluster_number="1" /tool="antismash" proto_core 10001..13571 /aStool="rule-based-clusters" /tool="antismash" /cutoff="10000" /detection_rule="((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)" /neighbourhood="10000" /product="azole-containing-RiPP" /protocluster_number="1" gene 167..946 /locus_tag="FB479_RS12845" /old_locus_tag="FB479_103624" CDS 167..946 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015890990.1" /locus_tag="FB479_RS12845" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103624" /product="TrmB family transcriptional regulator" /protein_id="WP_106655122.1" /transl_table=11 /translation="MIDELRKIGLSDLEARCYLVLHEEPKISGYEVAKKVSVSRTNVYA ALRALTEKGMCRTIESEPVLYDAVPIEQLIRLLQSEFDQTTRVLQEQLKSTPRTSPSFY NWQGDKAIETAIKRIISNANKSIVVDIWSEDVHWVEEPLIEAEKQGVAVTLISIGECHA KLKNVVVHKRSAEWNQVQSRKFSILCDAHSALLGSFGRGIKPTVLETDHPSLIELLQNA FYHDIIMGQIEKDFSPMLEEKYGSDYVRIITPYREYL" gene complement(1157..1972) /locus_tag="FB479_RS12850" /old_locus_tag="FB479_103625" CDS complement(1157..1972) /NRPS_PKS="Domain: Abhydrolase_1 (33-136). E-value: 2.2e-21. Score: 68.5. Matches aSDomain: nrpspksdomains_FB479_RS12850_Abhydrolase_1.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) PF00561" /gene_functions="biosynthetic-additional (rule-based-clusters) Abhydrolase_6" /gene_functions="biosynthetic-additional (smcogs) SMCOG1262: haloalkane dehalogenase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007717930.1" /locus_tag="FB479_RS12850" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103625" /product="alpha/beta fold hydrolase" /protein_id="WP_142065458.1" /sec_met_domain="PF00561 (E-value: 1.5e-17, bitscore: 59.8, seeds: 48, tool: rule-based-clusters)" /sec_met_domain="Abhydrolase_6 (E-value: 2e-18, bitscore: 63.5, seeds: 455, tool: rule-based-clusters)" /transl_table=11 /translation="MLQPQENGMVTRKYFQSGNVKLSYLDFGGDSQRVLLLLHGHMNDA RTFSEFASRFTDWRVIGLDQRGHGWSEHAPDTDYSREGYLNDILAFVQTVLDGAPVTIV GHSLGGVNAYQFAAHYPELVNAVIVEDIGVEIKADLSFAEKLPHRSPSLHDLRKSLEEV GVRAVDYFSESVFEDEQGWGFRSDLKGMPISQRHIDGEWWSDWLASSCPILLIHGQKSF VLDENQADRMVTQRPNTKLASFAECGHDIHSTDPDGYYRAVRSFLDELK" aSDomain complement(1565..1873) /aSDomain="Abhydrolase_1" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_FB479_RS12850_Abhydrolase_1.1" /evalue="2.20E-21" /label="FB479_RS12850_Abhydrolase_1.1" /locus_tag="FB479_RS12850" /protein_end="136" /protein_start="33" /score="68.5" /tool="antismash" /translation="VLLLLHGHMNDARTFSEFASRFTDWRVIGLDQRGHGWSEHAPDTD YSREGYLNDILAFVQTVLDGAPVTIVGHSLGGVNAYQFAAHYPELVNAVIVEDIGVEI" CDS_motif complement(1619..1675) /aSTool="nrps_pks_domains" /database="abmotifs" /detection="hmmscan" /domain_id="nrpspksmotif_FB479_RS12850_0001" /evalue="1.50E-05" /label="NRPS-te1" /locus_tag="FB479_RS12850" /protein_end="118" /protein_start="99" /score="17.5" /tool="antismash" /translation="PVTIVGHSLGGVNAYQFAA" gene 2086..3036 /locus_tag="FB479_RS12855" /old_locus_tag="FB479_103626" CDS 2086..3036 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016740491.1" /locus_tag="FB479_RS12855" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103626" /product="YafY family protein" /protein_id="WP_142065460.1" /transl_table=11 /translation="MPKSKRLLEMMMTVNRKRKFTVSELAAEFGVSGRTILRDLQELSE LGVPLYSEVGPHGGYHVLKERILPPIAFSEEEAVAIFFAIHALRHHTSLPFETESSSAL NKFYQFMPPDIRDRIDQMKRRVDFVTPKRQASSPHLSTLLDAAIQQKVLSITYESRGQQ STRDIQPIGIYTKNGLWYSPAYCFQQKHIRVFRCDRILSAEQSVSASKPLDLRDIHLEN RQVKTVEDQDTVHLFAELTTEGVQACEAELWLASMLHVRQDGSGWLEGNVARSDILFFS KFLIGLGKEVTVQHSPEILNGIKTYLAELINIYSQ" gene 3047..3382 /locus_tag="FB479_RS12860" /old_locus_tag="FB479_103627" CDS 3047..3382 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007717936.1" /locus_tag="FB479_RS12860" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103627" /product="hypothetical protein" /protein_id="WP_142065462.1" /transl_table=11 /translation="MALPKKGTRGITVGEQRYRWVISPAAKGLLALTIQHNEGMGQLIR VYVKSDVNDFWAEFPLVQHLDMRVIKPADVAAIIEEALKLGWQPDKKGAPLSFYFIGST LVKKGDT" gene 3433..4143 /locus_tag="FB479_RS12865" /old_locus_tag="FB479_103628" CDS 3433..4143 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_004225032.1" /locus_tag="FB479_RS12865" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103628" /product="DUF4274 domain-containing protein" /protein_id="WP_142065466.1" /transl_table=11 /translation="MDWATVSKTKDLEIVRAAVDQLDINERDQRGRTPLMLFITNRMPV EAIQMLLDKGTELEAEDKLGDTALKKAVKFKQVEAVKRLLQQGAELDSKHGILATAWNA ARMDKAIADLLLETKGAVRLTLSTEEEEAVDDILYEESEGKRRKKISQLDLSVLLHAVV NGYNWDDGPEAMIAAFENPACATITLLDMYELMDADYWLEMDEEETTESEERKRWRMLA IQLKEKLADEWDRG" gene 4149..4523 /locus_tag="FB479_RS12870" /old_locus_tag="FB479_103629" CDS 4149..4523 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007717939.1" /locus_tag="FB479_RS12870" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103629" /product="hypothetical protein" /protein_id="WP_142065468.1" /transl_table=11 /translation="MCRYGMYGPYKQIYACFPCRKVFKQTSKYELTEEEWNKIEHSCPQ CGEGMKDMGHDFKAPKQTDVKQWKKVEILYQHGFTYHSCGCGGPGYRPAQLNEVNEFLE KNKRFKTEGEKLLNRLTVSM" gene 4546..5742 /locus_tag="FB479_RS12875" /old_locus_tag="FB479_103630" CDS 4546..5742 /codon_start=1 /gene_functions="transport (smcogs) SMCOG1106: major facilitator transporter" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007717942.1" /locus_tag="FB479_RS12875" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103630" /product="sugar MFS transporter" /protein_id="WP_142065470.1" /transl_table=11 /translation="MATFFLIMIYLSFISLGLPDSLLGAAWPVMQAEYGAAFGVAGVIT IVISSGTIVSSLASGALLKRLGTGQITLISCLMTAAALLGFYFSPSIIWLMVLALPLGL GAGSVDAALNHYVATHYQAHHMSWLHCFWGVGATLGPIIMSGFIRDHGLWRNGYLTVSL IQFGLVILLAFTLPLWKRMENMQSHAVKESQGQPFGGQSEQPLRTKGVKLTLLSFLFYC GAEASVGLWGSSFLVNVKELPVAVAAVWVAMYYGGITVGRFITGFVTFKVSNRVLIRSG LLVSFAGAALLMLPLPTYYSQIGLLLLGLGFAPIYPCMLHDTPERFGKEQTQKLMGYQM AVAYTGATLLPPLLGWLATQTTVSILPFFTAFYIVFILYGSERVNRIMKQRAAQSETI" gene complement(5756..6355) /locus_tag="FB479_RS12880" /old_locus_tag="FB479_103631" CDS complement(5756..6355) /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_function="GO:0003700 - DNA-binding transcription factor activity [Evidence IEA]" /GO_process="GO:0006355 - regulation of DNA-templated transcription [Evidence IEA]" /codon_start=1 /gene_functions="regulatory (smcogs) SMCOG1057: TetR family transcriptional regulator" /gene_kind="regulatory" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017248321.1" /locus_tag="FB479_RS12880" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103631" /product="TetR/AcrR family transcriptional regulator" /protein_id="WP_106655129.1" /transl_table=11 /translation="MNRRRLTQAERKQETRQLLIDAAIETFAQLGFHGASVDKIADHAG FTKGAVYAHFQSKEDLFLAILEGQMQLHVSNIHHVIDHQPSLSHFIEAMDAYFMSVQQQ NRTWNMLNLEFLLYAMREESVRKRWSNMIIESVQHLSKAIQTLMPKESDSTLSADEMAW TILALENGMTIFYYMSGDQVPLSLYGKALQNMLSSK" gene 6536..7453 /gene="cyoE" /locus_tag="FB479_RS12885" /old_locus_tag="FB479_103632" CDS 6536..7453 /EC_number="2.5.1.141" /GO_component="GO:0016020 - membrane [Evidence IEA]" /GO_function="GO:0008495 - protoheme IX farnesyltransferase activity [Evidence IEA]" /GO_process="GO:0006783 - heme biosynthetic process [Evidence IEA]" /codon_start=1 /gene="cyoE" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017248322.1" /locus_tag="FB479_RS12885" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103632" /product="heme o synthase" /protein_id="WP_142065472.1" /transl_table=11 /translation="MQREESVATRNRPMSNILAQTVKTGIIKSNLIPMFAGLTLALYTY HFSLIDKLPEILFATIGSILIMGAAGAFNNVYDRDIDSVMERTKNRPTVTGEISTKTVL WLASLMTIFGLVALVLATPLAAIMGFLGLFFYVVPYTMWTKRRTVYNTEVGSISGAMPP LIGWAAIHPDITHPAILGLFIIMVIWQMPHFYAIAIRKHDEYKAAGVPMLPVVKGARRT YIQTNVYLVILIASSFLFWSLSAGLTFMALLLNILWLVLSVFGYRKMDSQKWSKTMFIY SLIHMTVLFSTVIIYSLMGIIFKL" gene 7633..7893 /locus_tag="FB479_RS31270" CDS 7633..7893 /codon_start=1 /inference="COORDINATES: protein motif:HMM:TIGR03601.1" /locus_tag="FB479_RS31270" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /product="heterocycloanthracin/sonorensin family bacteriocin" /protein_id="WP_106655131.1" /transl_table=11 /translation="MDDFKKELKKLKASEIKADKVQPWDKQKMNEATLVRGRCGCGRCG CGRCGCGRCGRCSCSCSCFCSCSCSCFCTCSCTCFFPCFIV" misc_feature complement(8033..8046) /note="TFBS match to ANR, Anaerobic transcriptional regulator, confidence: medium, score: 20.54" /tool="antismash" aSDomain 8061..8357 /aSDomain="Heterocycloanthracin_C_RRE" /aSTool="RREfinder" /database="RREFam.hmm" /description="RRE-containing C protein in a heterocycloanthracin LAP cluster" /detection="hmmscan" /domain_id="rrefinder_FB479_RS12895_Heterocycloanthracin_C_ RRE.1" /evalue="6.40E-43" /identifier="RREFam011.1" /label="FB479_RS12895" /locus_tag="FB479_RS12895" /protein_end="99" /protein_start="0" /score="135.6" /tool="antismash" /translation="MVKLNPSMRIKVKRDTVYLPDSDGSVYFRNNIGTFRMKGDMIERW VDQLMPMFNGEYTLAQLTDDLPEEYQQQIYQVAGTLLQNGFLQDISREKPHELS" gene 8061..10004 /locus_tag="FB479_RS12895" /old_locus_tag="FB479_103634" CDS 8061..10004 /GO_process="GO:0030152 - bacteriocin biosynthetic process [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) TIGR03693" /gene_functions="biosynthetic-additional (rule-based-clusters) Heterocycloanthracin_C_RRE" /gene_functions="biosynthetic-additional (rule-based-clusters) Thiopeptide_F_RRE" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016740498.1" /locus_tag="FB479_RS12895" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103634" /product="putative thiazole-containing bacteriocin maturation protein" /protein_id="WP_142065474.1" /sec_met_domain="TIGR03693 (E-value: 1.7e-180, bitscore: 596.9, seeds: 2, tool: rule-based-clusters)" /sec_met_domain="Heterocycloanthracin_C_RRE (E-value: 1.9e-41, bitscore: 135.6, seeds: 5, tool: rule-based-clusters)" /sec_met_domain="Thiopeptide_F_RRE (E-value: 1.5e-12, bitscore: 43.0, seeds: 20, tool: rule-based-clusters)" /transl_table=11 /translation="MVKLNPSMRIKVKRDTVYLPDSDGSVYFRNNIGTFRMKGDMIERW VDQLMPMFNGEYTLAQLTDDLPEEYQQQIYQVAGTLLQNGFLQDISREKPHELSPEIIS AYAEQIEFLDQFGGSGGYLFQKYRTEKVLIVGAGSFLLSIAHALLESGCVQFHYVITNE APTKRERLDAIIEHARRKDPHVKLEELKPASWGIAEWSEAIEPFSAVLYTSAQVNKDEI SVIHSACRERKKVFVPAVCLMQKGIAGPLIHPESEACLESAWRRLHRQTLCADPDQHPF SMTAAAMLANVAVFEWFKHVTGVNDNEDENKVYVLNLETLEGSWRSFLPHPMVKEGMRL EVLDRKELESRLSNETEPKEGLQSLFQSWTSSDTGIFHSWDEGELTQLPLTQCNVTAVD PVSEGPAELMPQIVCPGYTHEEARREAGLLGAEMYVGRMVNHLAAIDSAFRNVGNGNIG VGAGETAAEGLNRALRHCLTLTYAADMKGRQPTARPVHTDTIADERCQFYIRSFTTLGE KPTIAKGHEVFGFSVYWVETSTGWYGSIGLQEREALRSALMYALQQIQTPTNQMSKYVL TAQSVGLTGEAIEVARNSVQKESEKEVWKQAVEVLALHHLSPRVMNAAMEPFLEEDLGG VFALLLSREVPR" aSDomain 8082..8324 /aSDomain="Thiopeptide_F_RRE" /aSTool="RREfinder" /database="RREFam.hmm" /description="RRE-containing F protein in a thiopeptide cluster" /detection="hmmscan" /domain_id="rrefinder_FB479_RS12895_Thiopeptide_F_RRE.1" /evalue="5.10E-14" /identifier="RREFam004.1" /label="FB479_RS12895" /locus_tag="FB479_RS12895" /protein_end="88" /protein_start="7" /score="43.0" /tool="antismash" /translation="MRIKVKRDTVYLPDSDGSVYFRNNIGTFRMKGDMIERWVDQLMPM FNGEYTLAQLTDDLPEEYQQQIYQVAGTLLQNGFLQ" gene 10001..11941 /locus_tag="FB479_RS12900" /old_locus_tag="FB479_103635" CDS 10001..11941 /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) azole-containing-RiPP: TIGR03604" /gene_functions="biosynthetic (rule-based-clusters) azole-containing-RiPP: YcaO" /gene_functions="biosynthetic (rule-based-clusters) azole-containing-RiPP: TIGR03882" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_016740499.1" /locus_tag="FB479_RS12900" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103635" /product="TOMM precursor leader peptide-binding protein" /protein_id="WP_142065476.1" /sec_met_domain="TIGR03882 (E-value: 1.9e-47, bitscore: 157.1, seeds: 35, tool: rule-based-clusters)" /sec_met_domain="YcaO (E-value: 3.7e-76, bitscore: 252.6, seeds: 127, tool: rule-based-clusters)" /sec_met_domain="TIGR03604 (E-value: 8.5e-124, bitscore: 408.8, seeds: 25, tool: rule-based-clusters)" /transl_table=11 /translation="MSADILVIGDGVLAELVSERLRKDYRVWHSENMESATSGADLALL LQDTYHPALHLQAEKHFRQTNIPWLRGFVSFGEAIVGPLVLPGQTGCSQCADIRRLMAG RDRKEMWKVQHMFSEKGGIPRDPWSSRSGLLQVAFHVSAEVERIVEGRQAQLSEHIQFI DLQTLTSTRHFFLPNPLCPVCSRLPEDSEEQARVRLKPIVKQSPDSYRTKPIADLKQAL PTEFLDSRTGFLNGKVRDLVSVFADVSVNLPLFSHDEGTAGRSVSYADSEITAILEGME RYCGLMPRGKRTMVRDCYANLAPHALDPTTAGTHSEEQYALPDFPFQPFDPKQDMNWVW GYSFKEERPILVPELMAYYSMGFGEGVVFETSNGCALGGSLEEAIFYGILEVVERDAFL MTWYAKLPLPRLDPASVNDRELLLLIDRMQTVTNYDLYLFDATMENGIPAVWTIAKNRG EAGLHLVCAAGAHPDPIRAVKSSIFETAAMLMPLTDKYEANRDKYLRMLHDSSLVKKMD DHAMLYSLPEAAERLSFLMDDSRPMVSFAEQFPKRKQHRDLTDDLRELLGVFQKLDLNV IVVDQTTPEVERHGLSCVRVLIPGMLPMTFGHHLTRVTGLKRALEVPVKLGYAKQPLRV EELNPFPHPFP" gene 11964..13571 /locus_tag="FB479_RS12905" /old_locus_tag="FB479_103636" CDS 11964..13571 /GO_function="GO:0016491 - oxidoreductase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic (rule-based-clusters) azole-containing-RiPP: PF00881" /gene_kind="biosynthetic" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017248326.1" /locus_tag="FB479_RS12905" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103636" /product="SagB family peptide dehydrogenase" /protein_id="WP_142065478.1" /sec_met_domain="PF00881 (E-value: 1.1e-06, bitscore: 24.5, seeds: 78, tool: rule-based-clusters)" /transl_table=11 /translation="MSLENFLYRLHFDTENARPSDLEVDWEDAPLTYKLYRGLPVIPLP EDVPLSLIDQTVSQEMSLPQLGAWLWYSYGLIQISQSLLTTIPGDAGSESFLPLVTNRR PVPSGGGLYPSELYVYLKLSSIPAGLYHYDVAHHRLVRLREGNFDDVLSRALGGHCPVH ACFGVAFVSIYFWKNFFKYDEFSYRLQGLDAGVLLGQLQEMANQLGVTNAVHYQFLDRA MNALLGLSVDQESVYAVVPLSLQTEGERHSHSPAHWAERGEETASELIRELPEQKHVHV MRSKRMRQYPVLLRMNEAAMQESPHSFTSYSDHLENKWTRRSEERILLPRLEKPSYDLA QFSRERFSPEMDFIRRKTGRDQVARIMEMAMSVLSYGNDLGKNQEVAWRRLSLLVSIHQ VEGIQDGAYAYEPLEHALYPIVYGDLRARLQDGMTLDNVNLYQVPLCFHVAGKRDHLIN QLGFRGYRIQQMEAGIVVHRLLLAAFTCGMGGHPLLGFDVKNCDDLYNLETEEKTCLIQ IPVGHYRPRARFQGGLHG" gene complement(13613..15358) /locus_tag="FB479_RS12910" /old_locus_tag="FB479_103637" CDS complement(13613..15358) /GO_function="GO:0003824 - catalytic activity [Evidence IEA]" /NRPS_PKS="Domain: Aminotran_5 (197-571). E-value: 2.7e-66. Score: 215.9. Matches aSDomain: nrpspksdomains_FB479_RS12910_Aminotran_5.1" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Aminotran_5" /gene_functions="biosynthetic-additional (rule-based-clusters) DegT_DnrJ_EryC1" /gene_functions="biosynthetic-additional (smcogs) SMCOG1139: aminotransferase class V" /gene_kind="biosynthetic-additional" /inference="COORDINATES: protein motif:HMM:TIGR01976.1" /locus_tag="FB479_RS12910" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103637" /product="cysteine desulfurase-like protein" /protein_id="WP_142065480.1" /sec_met_domain="Aminotran_5 (E-value: 4.7e-64, bitscore: 212.3, seeds: 34, tool: rule-based-clusters)" /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.2e-08, bitscore: 29.4, seeds: 38, tool: rule-based-clusters)" /transl_table=11 /translation="MSTTLNTELVRIQPWADHDLPLLHLLNAPAMMEHVGGPETEEQLL ARHERYVNKDGQQSSKMFSIVLLPDHIPVGSIGYWDRIWQGEHVYEVGWSVLPPFQGKG IASAALASLITRINQERRHKFIHAYPSVDNPASNAICRKLGFTLQSECVFEYPPGSFMR SNDWRLEVASEHYPIEKVREQFPALQRTYEGKPVVYLDGPGGSQVVKSSMDAIYRFMAN GGANLHGSFPTSKETEAILADAKQAVADLFHVRPKEVAFGANMTTLTFAIARALGRHWK EGDEIIVSEMDHRANVDPWLTVAADRGMTVRWLKVNKETLTLQLDELDTLLTSRTRLVA VGLASNAVGTIHDLAAISQKARAKGVIFAVDAVHAVPHFSIHRDEIGADILLCSAYKFF GPHVGIAVIRKEIFEALDVYKLVPAPSSYPDKLETGTQNHEGIAGIRPAIEFVASLGFG DSFAEKIRSGYEMIEAYENHLADKLRKELSAMKGVTLYQAGEDVPKTPTVAFRIEGFSP REICERLCEEHSIFVADGDFYATTLANCLGINESGGWIRAGLAPYNTHEEIDRFIQGIK AIIRT" aSDomain complement(13646..14767) /aSDomain="Aminotran_5" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_FB479_RS12910_Aminotran_5.1" /evalue="2.70E-66" /label="FB479_RS12910_Aminotran_5.1" /locus_tag="FB479_RS12910" /protein_end="571" /protein_start="197" /score="215.9" /tool="antismash" /translation="VYLDGPGGSQVVKSSMDAIYRFMANGGANLHGSFPTSKETEAILA DAKQAVADLFHVRPKEVAFGANMTTLTFAIARALGRHWKEGDEIIVSEMDHRANVDPWL TVAADRGMTVRWLKVNKETLTLQLDELDTLLTSRTRLVAVGLASNAVGTIHDLAAISQK ARAKGVIFAVDAVHAVPHFSIHRDEIGADILLCSAYKFFGPHVGIAVIRKEIFEALDVY KLVPAPSSYPDKLETGTQNHEGIAGIRPAIEFVASLGFGDSFAEKIRSGYEMIEAYENH LADKLRKELSAMKGVTLYQAGEDVPKTPTVAFRIEGFSPREICERLCEEHSIFVADGDF YATTLANCLGINESGGWIRAGLAPYNTHEEIDRF" gene 15534..16046 /locus_tag="FB479_RS12915" /old_locus_tag="FB479_103638" CDS 15534..16046 /EC_number="2.3.-.-" /GO_function="GO:0008080 - N-acetyltransferase activity [Evidence IEA]" /GO_function="GO:0016746 - acyltransferase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (smcogs) SMCOG1111: GCN5-related N-acetyltransferase" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015891003.1" /locus_tag="FB479_RS12915" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103638" /product="GNAT family N-acetyltransferase" /protein_id="WP_106655136.1" /transl_table=11 /translation="MNEKSIHTQRLILIPFTHRIATNILQENYEELLDMGLTLGKGWPD EDAMETIPKIIKALELAGEPTGFESWMIITKDGRKIIGDAGFKGAPNTEGEVDIGYGII EAERKKGYAYEAAEGLANWALLQPDVKKITAKCLLDNIDSAKLLVKMGFTEIKRDDTMI YWSSQKG" gene 16199..16810 /locus_tag="FB479_RS12920" /old_locus_tag="FB479_103639" CDS 16199..16810 /GO_process="GO:0055085 - transmembrane transport [Evidence IEA]" /codon_start=1 /gene_functions="other (smcogs) SMCOG1188: hypothetical protein" /gene_kind="other" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007717970.1" /locus_tag="FB479_RS12920" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103639" /product="DedA family protein" /protein_id="WP_106655137.1" /transl_table=11 /translation="MQNWMTQIIEQYSYFGILLMMALENIFPPIPSEVILTFGGFMTTQ SSLTVFGVILSATIGSVIGAIMLYGIGFYLDVQKIEKIVERWGHLLRIKTADIHKANEW FDRYGYWTIFFCRMIPLIRSLISIPAGMTKMNFPLFLLFTTLGTFIWNIVLVMAGHLLG ESWEDILYYFDLYSNVVYVVLAFTGVAFLIFFLRKRKADA" misc_feature 16887..16903 /note="TFBS match to HexR, Glucose-responsive regulator, confidence: weak, score: 26.36" /tool="antismash" gene 16994..18478 /locus_tag="FB479_RS12925" /old_locus_tag="FB479_103640" CDS 16994..18478 /GO_function="GO:0016787 - hydrolase activity [Evidence IEA]" /codon_start=1 /gene_functions="biosynthetic-additional (rule-based-clusters) Amidohydro_1" /gene_kind="biosynthetic-additional" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_017248331.1" /locus_tag="FB479_RS12925" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103640" /product="amidohydrolase family protein" /protein_id="WP_185746361.1" /sec_met_domain="Amidohydro_1 (E-value: 1.8e-33, bitscore: 111.8, seeds: 57, tool: rule-based-clusters)" /transl_table=11 /translation="MIDLLLVDGVVITMDKDRRVLQDGAVAIDKGRILEVGDTPLLKDK YPAQKVVDCAHHCILPGFVDAHGHGGHSLFKTIATDNINDWMPIMTNTYKHYVTDDFWY YEGKLSALERLKAGITTGVSVLGSMPRSDDPIFALNHAKAYNEVGVREIVATGPCNPPW PHPFSRWVDGQKVTKEVTYEQVLAGAEAVIEALNHANNDKTRAYITPFVIVTSVNPSYP TPADQLFGLTDFDRYQARKIREIAKKYDTRIHSDAFGGMIHLAIQDKEYALLGPDVHLQ HCRGISFDEAKILAETGTNVSASPGFGQINARTPITELLEMGATVAITTDGTSPMTPFD MFQAMRRMQLLQQAALRDYYYLPPGKLLEMVTIDAARCVGWDDELGSLEAGKKADVITV NMHQAHLNPENMHVHRVVYQAVGGDVNHVIVDGELIMDERRVLTVDEKQILKEANEEAN RTIERAGLEIYMQPSKYFWGHARAYLDERRFDPTKL" misc_feature complement(18559..18568) /note="TFBS match to AbrC3, Antibiotic production activator, confidence: strong, score: 18.07" /tool="antismash" gene 18665..19507 /locus_tag="FB479_RS12930" /old_locus_tag="FB479_103641" CDS 18665..19507 /EC_number="7.6.2.14" /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_function="GO:0016887 - ATP hydrolysis activity [Evidence IEA]" /codon_start=1 /gene_functions="transport (smcogs) SMCOG1000: ABC transporter ATP-binding protein" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007717977.1" /locus_tag="FB479_RS12930" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103641" /product="ABC transporter ATP-binding protein" /protein_id="WP_142065482.1" /transl_table=11 /translation="MYLTIDNVTKHFENEQKQKVKVLDDINLEVEKGSFVSIVGPSGCG KSTLLYLIAGLDRPDSGDIHVAGNKVNKPGPDRVVVFQEAGLFPWLTVLENVTYGLKLK KMSAAKANEKALEVLKMVHLSKYVHSYPHQLSGGMKQRVAIARALVMEPDILLMDEPFS ALDEQTRMVLHKELLEIWRTTKVTIFFVTHNIREAVQLGEKIVVFATRPGKIKEIIAVP AMRDGVMPDSVTLSTEQKVLSILQEEIEKVLKEEMGNDYSFKTGHLHRYDSGDLGSHI" gene 19440..20195 /locus_tag="FB479_RS12935" /old_locus_tag="FB479_103642" CDS 19440..20195 /GO_function="GO:0005524 - ATP binding [Evidence IEA]" /GO_function="GO:0022857 - transmembrane transporter activity [Evidence IEA]" /codon_start=1 /gene_functions="transport (smcogs) SMCOG1044: ABC transporter, permease protein" /gene_kind="transport" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_020372021.1" /locus_tag="FB479_RS12935" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103642" /product="ABC transporter permease" /protein_id="WP_020477521.1" /transl_table=11 /translation="MTTALRRVIFIAMIAAIWEVTSRLSGLPSFMFPSLTQVFETLVNG LISGQITAAIGKSMGRILLGFLIAIIVGLILGYFIWRYKLVEDTLGFVVTALQSIPSIV WFPLAIIWFGLNDFSILFIVTIGATWTMTVNATSGFKNVPPLYQRVAKTYGSSGFHFVR TVILPASVPQIISGLRIAWAFSWRALMAGELLGGGGGLGQLLEMGRSLGQMDLVISVMI IIAIIGTIVDNVVFSRIERNVQMKWGVHS" gene 20227..21222 /locus_tag="FB479_RS12940" /old_locus_tag="FB479_103643" CDS 20227..21222 /GO_component="GO:0016020 - membrane [Evidence IEA]" /GO_component="GO:0043190 - ATP-binding cassette (ABC) transporter complex [Evidence IEA]" /GO_function="GO:0042626 - ATPase-coupled transmembrane transporter activity [Evidence IEA]" /GO_process="GO:0042918 - alkanesulfonate transmembrane transport [Evidence IEA]" /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_007717982.1" /locus_tag="FB479_RS12940" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103643" /product="aliphatic sulfonate ABC transporter substrate-binding protein" /protein_id="WP_142065484.1" /transl_table=11 /translation="MLKKVLSTVVASLLLMGVASGCSTGGAESSEKEVRIGYFPNLTHS ATIIALEKGYFKEAFGADVKIQTKTVTNGGLFMEAMATKAIDVGTVGPGPLLNFYVKHP GYRLISGAVNGGAVLVMNGSTNITELKDLKGKKIAIPVIGSTQDVMLRKALNEVGLKPT TNGGDVELYAAAPADTAALFVQKSVDGAATQEPWGYVLENQAEGKLLLDWDQFAWGKES TNTVVAASDEFLKREGLATAYLQAHKKAVKFIQENPEESQDLIIKHLKGLTGKELSKKE VQAAFSRLEVTTAVNEKVIQEMADISQEAGYISSNKIDGLIDLKYLEAVK" gene 21302..22162 /gene="kynA" /locus_tag="FB479_RS12945" /old_locus_tag="FB479_103644" CDS 21302..22162 /EC_number="1.13.11.11" /GO_function="GO:0004833 - tryptophan 2,3-dioxygenase activity [Evidence IEA]" /GO_process="GO:0019441 - tryptophan catabolic process to kynurenine [Evidence IEA]" /codon_start=1 /gene="kynA" /inference="COORDINATES: similar to AA sequence:RefSeq:WP_015891010.1" /locus_tag="FB479_RS12945" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103644" /product="tryptophan 2,3-dioxygenase" /protein_id="WP_142065486.1" /transl_table=11 /translation="MRPHEQGSNQPNDTSLETQIHTDFQKEMTYGDYLQLDQILSSQQC QSSHHDEMLFIIIHQVSELWMKQILHELSAANECICNHDLEPAFKMFARVSRIQQQLIK SWDVLSTLTPADYLQFRDKLGHSSGFQSYQNRLIEFTLGYKNQHVLAVYAHQPELHAQM SAALQQPSIYDAAIREMAARGLPIDPECLNRDWSQPYQPNQSVEQAWLTVYRNVNQYWD LYELAEKLVDIASQQQQWRFNHMTTVERIIGQKPGTGGSSGVMYLRRALDHRFFPELWS LRTLL" misc_feature complement(22291..22304) /note="TFBS match to LexA_variant_2, Repressor of DNA damage response, confidence: weak, score: 19.65" /tool="antismash" misc_feature 22291..22304 /note="TFBS match to PsrA, Oleic acid responsive regulator, confidence: weak, score: 20.31" /tool="antismash" gene 22362..23462 /locus_tag="FB479_RS12950" /old_locus_tag="FB479_103645" CDS 22362..23462 /codon_start=1 /inference="COORDINATES: similar to AA sequence:RefSeq:WP_018766749.1" /locus_tag="FB479_RS12950" /note="Derived by automated computational analysis using gene prediction method: Protein Homology." /old_locus_tag="FB479_103645" /product="AAA family ATPase" /protein_id="WP_185746362.1" /transl_table=11 /translation="METMKPPMEVLYAKQLNALRSHDTGTKPPNWLMSPRAVRDFILGT DEPLSYEGESITITKKFYGDDVLIERAIVTLAGNRGLMLVGEPGTAKTMLSELLSAAIS GTSTNTIQGTAGTTEDMIKYSWNYAMLLDKGPSLQALVPSPLYTGMSKGIITRFEEITR CPFEVQDVLISILSDKVMNIPELSDGILFAKPGFNIIATANLRDKGVNEMSSALKRRFN FETIAPINHVKMEAQIIESQAKVILEQSGVNIEIDRDVVEILATTFMELRMGETKEGFK IDSPQSVMSTAEAVSVYVQSAMTSHYYDGRPISMDRLVQNMLGAVVKENQKDANILKTY FTKVVKERAKEEGLWGTYYNEKKWIK" ORIGIN 1 atcctatgtg agtatgttac gagttttcaa tggggagata gtcttgtgga gggaatattt 61 caatccgatt gagttgcaga aggtgctgga ttcataggac tgttgacttt ctcacgggac 121 aatagcagta ttcatatata ctactaaaca aaaaggaggt aacaacttga ttgatgagct 181 ccgaaaaatc ggattgtcag acctagaagc cagatgttat ttggtgcttc acgaggaacc 241 caaaatatct ggatatgaag tggccaagaa agtttccgtc tctcggacaa acgtatatgc 301 tgcacttcga gcattaacgg aaaagggcat gtgccgcaca atcgaatcgg agcctgttct 361 ttatgatgca gtaccgatcg agcagctgat tcgtttgtta caatcggaat ttgatcaaac 421 aacccgtgtc cttcaagagc agttgaaatc cactccccga acgtctcctt ccttttataa 481 ttggcaaggt gacaaagcga ttgaaacagc catcaaacgt atcatctcaa atgcaaacaa 541 aagcatagtt gtcgatattt ggtctgaaga tgttcattgg gtagaggagc cactgataga 601 agcggaaaaa caaggggttg ctgtcacgct aatatccatt ggcgaatgcc atgcaaagct 661 aaaaaatgtt gtcgtacata aaagaagcgc ggaatggaat caagtgcaat ccaggaagtt 721 ctctatactt tgcgatgcac attcagcgtt gctgggcagc tttgggcgtg ggatcaagcc 781 aactgtactg gagacggacc acccatctct cattgaattg ctgcaaaatg cgttctacca 841 tgacataatc atgggtcaga tcgagaagga tttcagcccc atgctggaag agaaatatgg 901 gagtgactat gtacgcatta tcacacctta cagggaatac ttataacgag tatttcctct 961 ttttatcaac aaaacagtgg cttcaagtac gaagaagact ctcgtaatca accaaaagag 1021 aattgaacac gtaaagctaa taatattcat ttacagccaa tccttagagg gttggctgtt 1081 tgttatggag gagccttaaa aagggaggta gagactttcc gatgtctctt cccccctttt 1141 atgcgtgcat cgtctattat ttcaactcgt cgagaaagct tcttacagcc cgatagtatc 1201 cgtcagggtc cgtagaatgg atatcatgcc cgcactcagc aaaacttgca agctttgtgt 1261 ttggtctttg cgtcaccatg cgatcggctt gattttcatc caatacaaag cttttttggc 1321 catgaatcag caagatcggg caagaagacg ctagccaatc gctccaccat tctccgtcaa 1381 tgtgtcgttg agaaataggc atgcctttta aatcagaacg gaatccccag ccttgttcat 1441 cctcaaacac gctttccgaa aaataatcga ccgcccgaac gccgacttct tcgagtgatt 1501 ttcttagatc gtgtaaagaa ggggagcgat gtgggagttt ttctgcaaag gataagtcgg 1561 ccttaatttc tactccgata tcttccacaa tgaccgcgtt cacaagctcc ggataatgag 1621 cagcaaattg ataagcgttg acacctccta aagaatgccc aacgatcgta acaggagctc 1681 cgtccaaaac ggtctgaaca aaggcgagaa tgtcgttgag gtagccttca cgtgaataat 1741 ccgtatctgg ggcatgctca ctccagccgt gaccgcgttg atccaatcca atcacgcgcc 1801 aatctgtgaa tctggaagca aactctgaaa atgttcgtgc atcattcata tgaccatgca 1861 gcaggagcaa aactctttga ctgtccccgc caaaatcgag gtaagatagt tttacgttac 1921 ctgattggaa atattttctc gttaccatac cgttttcttg tggctgaagc atacacaatt 1981 cctccttatt tatcatcagc atcatgtcca actcgacttc tccttcacca taacagataa 2041 tagtgacagg aattgtcata ataaaaacat gtggagagtt atgtcatgcc gaaatcaaaa 2101 cgactgctgg aaatgatgat gactgtcaac cggaagcgca agtttactgt gagtgagctt 2161 gctgccgaat ttggcgtatc agggcgaacg attctaaggg acttacaaga gctgagtgag 2221 ttaggggtac ctttgtactc ggaggtggga ccgcatggtg gatatcatgt tttgaaggaa 2281 aggattttgc cgccgattgc attctcagag gaggaggcgg tcgcaatctt tttcgcgatc 2341 catgctcttc gacaccatac ctctctgccg tttgagacag aatcctcttc ggcgctgaac 2401 aaattttatc aatttatgcc gccggacatt cgtgatcgca ttgatcaaat gaaacgccgc 2461 gttgatttcg tgacaccgaa gcggcaagcc agctctccgc atttatccac tctgctggat 2521 gccgccatcc aacaaaaagt gctcagcatc acttatgaat cgcgcggaca acaatccaca 2581 cgggacatac agccgattgg aatttataca aaaaacggtc tatggtacag cccggcttat 2641 tgttttcaac agaaacatat tcgcgtgttt cgttgtgacc gtatcctctc tgccgaacaa 2701 tcggtatcgg catcgaagcc gttggatctt cgagacattc acttggaaaa tagacaggtc 2761 aaaaccgtcg aggatcagga caccgtccac ttatttgcag agctgaccac tgagggtgtg 2821 caggcttgtg aggctgaact ttggcttgca tccatgctgc atgtgcggca ggatgggagt 2881 ggttggttgg aaggaaatgt agcgagaagt gacatcttgt ttttttcaaa gtttttgatt 2941 gggttgggga aagaagtgac ggtccagcat tcacccgaaa tattgaatgg aataaaaacg 3001 tatttggcag aactgataaa catttactct caatgaggag gcagctatgg cattacccaa 3061 aaaaggcaca cggggtataa cggtaggaga gcagcgatat cgttgggtca ttagcccagc 3121 agcgaagggt ctgcttgctt tgacgattca gcacaatgaa ggaatggggc aactgattcg 3181 cgtgtatgtc aaatcagatg tgaatgattt ttgggcggag tttccacttg tgcagcattt 3241 ggatatgaga gtgataaagc cagctgacgt tgcggctatc attgaagagg cgctcaagct 3301 ggggtggcag cctgacaaaa agggggcgcc tttgagcttt tacttcatcg gtagtacgct 3361 tgtcaaaaaa ggggatacat agcaataccg gaacgggagt tgtctataga gaagaagagg 3421 agaggaagcc ggatggattg ggcaaccgtc agcaagacaa aggatcttga gatcgtgcga 3481 gcagcggtgg atcaattgga tatcaacgaa cgagatcaac gcggacgaac accgctgatg 3541 ctttttatca caaaccgaat gccggtggaa gcgattcaga tgctattgga taaaggcaca 3601 gagctggagg cggaagacaa acttggtgat acagcattga aaaaagccgt caaattcaag 3661 caagtcgaag ccgtaaaacg attgctgcaa cagggagctg agcttgattc taagcatggc 3721 attttggcga ctgcttggaa tgcggccagg atggataaag ctatagcaga cctgctgctc 3781 gaaacgaaag gcgccgtacg tctaacactg tctactgagg aagaggaagc ggtggacgac 3841 attttatatg aggaatctga gggcaagcgg cgcaagaaaa tcagtcagct tgatttatct 3901 gttcttcttc acgctgtggt gaatggctac aactgggatg acggacctga ggcgatgatt 3961 gcggcttttg agaatcctgc ctgtgcaacg atcactcttt tggatatgta tgagttgatg 4021 gatgccgact attggttgga gatggacgag gaagaaacta cggaatcgga ggagcgaaaa 4081 cgttggagaa tgctcgccat ccaattgaaa gagaagctgg cagatgaatg ggataggggt 4141 tgatcatcat gtgtcgatat gggatgtatg gtccgtacaa acaaatatac gcctgtttcc 4201 cttgccgaaa agttttcaag caaacctcca agtatgagct gacggaggaa gagtggaaca 4261 agatcgagca tagctgtccg caatgtggtg agggaatgaa ggatatgggg catgatttca 4321 aggctccgaa gcaaacggat gtgaagcagt ggaaaaaggt tgaaatcctc tatcagcatg 4381 ggtttaccta tcattcctgt ggctgtggtg gtccaggcta ccgaccagcc caattaaatg 4441 aagtgaacga atttctcgaa aagaacaagc ggttcaaaac agagggagag aaattgctga 4501 atagactcac agtaagtatg taaaaacgac cagggggcgg ggatcgtggc aacattcttt 4561 ttgatcatga tttacttgtc gtttatcagc ttgggccttc ctgattcgtt actgggggca 4621 gcttggccgg tcatgcaagc agaatatggt gcggcattcg gtgtagcggg tgtgatcacc 4681 attgtgattt catctggtac gatcgtgtca agcttggcaa gcggagcttt attaaaacgg 4741 ctcggtactg gacagattac gctgattagt tgcttgatga cagcggcagc cttgctcggc 4801 ttttattttt ccccttccat tatatggttg atggtattgg cattgccgct tggactcggt 4861 gctgggtctg ttgatgctgc actgaatcat tatgtcgcca cgcattacca agcacatcat 4921 atgagctggc tgcattgctt ctggggtgtg ggtgctacgc ttggccccat tattatgtct 4981 ggttttattc gtgatcatgg tttatggcgg aatggatact tgaccgtttc gcttatacaa 5041 tttgggttag tcattctgct agcgtttaca cttcctttgt ggaaacggat ggagaacatg 5101 cagagccatg ctgtcaaaga gtcacagggt cagccgtttg gtgggcagag cgaacaaccg 5161 ttacggacaa aaggcgtcaa gctgactttg ctgagctttc tcttctattg tggggctgaa 5221 gcatcggtgg gattatgggg gagcagcttt ttggtaaatg tgaaagagct acccgtggct 5281 gtggcggcgg tgtgggtagc catgtattat ggtggcatta ctgttgggcg atttatcacg 5341 ggctttgtta ccttcaaagt gagcaaccgc gtcctgattc gcagtggact tcttgtatca 5401 ttcgctgggg cagcgctgtt gatgttgcca ttaccaacct actattcgca gatcggttta 5461 ctgttgctcg ggctaggctt cgctccgatt tatccctgta tgctgcatga tacccccgaa 5521 cgctttggga aagagcaaac gcaaaagctg atgggctatc agatggcggt tgcgtacacg 5581 ggtgccacac ttctgcctcc gttgctaggg tggcttgcta cacagacgac cgtttcaatt 5641 ttaccgtttt ttacagcctt ctacattgtg ttcatactct atggttctga gagagtcaat 5701 cggatcatga agcaacgggc tgctcagtca gaaacgatct agcatagggc actcgctatt 5761 ttgacgatag catgttttgc aaagcttttc cgtataaaga caaggggacc tggtcgccgc 5821 tcatgtaata aaagatggtc atgccattct caagagccag aatcgtccat gccatttcat 5881 ccgcggatag agtagagtca ctttcttttg gcatcaatgt ctgtatggcc ttggaaagat 5941 gctgtaccga ctctatgatc atgttcgacc atcgcttgcg aacagattct tcgcgcatgg 6001 cataaagcaa aaattccagg ttcagcatgt tccacgtgcg attttgttgc tgaacggaca 6061 taaaataggc gtccatggcc tctataaaat gcgataatga tggctggtga tcaattacgt 6121 gatggatatt ggacacatgt aattgcattt gaccttccaa aatagcgaga aagagatctt 6181 ctttggactg gaaatgagca taaacagctc ctttggtaaa accagcatgg tctgcgattt 6241 tatcaacgga agccccgtgg aagcctaact gagcaaaggt ctcaattgct gcgtctatga 6301 gcaattgcct tgtttcctgt ttacgctctg cttgggttaa tctgcgtctg ttcattcgtc 6361 cgcctccttc tcttgacaat cataccatat agaattaaca ttccatatag tatttaaatc 6421 cttattggta tgtaagatac aaatggcagt tttgtaggat ttaaccgtct actatgtcat 6481 gctattttgg agtacgatag atatagatca tctagtaaga ggggacagtg tacgaatgca 6541 gagggaagaa agcgttgcaa cgcggaatcg gcctatgtcc aatattttgg cgcaaacggt 6601 taagacgggg attatcaaat cgaatctgat tccgatgttt gccggattaa cattggcttt 6661 atatacgtat cacttcagtc taatagataa gctgccagaa atcttatttg ctacaattgg 6721 ctctatttta atcatgggcg cagcgggtgc atttaataac gtgtatgatc gcgatatcga 6781 ttctgtcatg gagcggacga aaaacagacc tacggtgaca ggggaaatat caacgaagac 6841 agttttatgg ctcgcctcct tgatgacgat ttttggcttg gtggctcttg tgttggcaac 6901 accgttggct gctattatgg gttttctcgg actgtttttt tatgtcgtgc cttatacgat 6961 gtggacaaaa agaagaacgg tttacaatac ggaggtaggg agtatttctg gcgcaatgcc 7021 gccgctcatc ggttgggcag ccattcaccc agatatcaca catcctgcta tcctcggtct 7081 ctttatcatc atggtcattt ggcagatgcc tcacttctat gcgatcgcga ttcgcaagca 7141 tgatgagtac aaagcggctg gggttcctat gttgcctgtg gtcaaaggtg caagacgtac 7201 ctacattcaa acaaatgtct atttggttat tttaattgcc agcagttttc tattctggtc 7261 attgagcgct ggtcttacgt ttatggccct gctcctgaat attctatggc tggttctgag 7321 tgtttttgga taccggaaaa tggattctca gaaatggtcg aaaacgatgt ttatttattc 7381 gctgatccat atgacggtgc tgttctcaac ggtgattatt tactcgctca tggggattat 7441 cttcaaactg taaatcagtg atcatccaag ctgccttcac tttgagggcg gtttcttttt 7501 tgtctgggaa tcttgcagca tagctggttc ttctcatgtt caaccaaaaa tgggcgcctg 7561 atcatggaac aaatacccaa tgtcgcttca taaatttaga gtagctccat gaagtggcgg 7621 aggtgaagat gaatggatga tttcaaaaaa gagctaaaaa aactaaaggc ttccgaaata 7681 aaagcggata aagtgcagcc ctgggataaa caaaagatga atgaagcgac gcttgttcgc 7741 gggcgatgtg gatgtggtag atgtggatgt ggtagatgtg gatgcggccg ttgcgggcgt 7801 tgtagttgca gttgttcctg tttctgctcc tgtagctgtt cctgcttctg tacgtgcagc 7861 tgcacttgct tcttcccatg ctttatcgtc taaatggcga tattttctaa ccacgatccg 7921 aatgaagccc cctgccagaa tgacggattg gcaggggctt tgtttttctt gctggcatgt 7981 gagacaaagt attctacata aaatgactgt gacacatcat gtagcaaaag ggctgatctg 8041 gcacaaaaag ggggaggaga atggttaagc tgaatccttc gatgcgcata aaagtgaagc 8101 gggatacggt ttatctgcct gattcagatg ggagtgtata ctttcggaat aacatcggaa 8161 catttcgaat gaagggggac atgattgaac gatgggttga ccagctcatg ccgatgttta 8221 atggtgagta cacgctggca caattgaccg acgatttgcc ggaagaatac caacagcaaa 8281 tataccaggt agcgggtacg cttttacaaa atggatttct tcaagacatc agtcgagaga 8341 agccacatga actatcgcca gaaatcatat cggcctatgc ggaacagatt gaatttttgg 8401 accagtttgg cggatcgggt ggatatttgt ttcaaaaata tcgtacggaa aaagtattaa 8461 tagttggggc aggttccttt ctcctttcta ttgcccacgc cttgctagaa tcaggctgcg 8521 ttcaattcca ttatgtcatt acgaatgaag ctcctacgaa gcgggagcga ttagacgcaa 8581 tcatagagca tgctcgcagg aaagacccgc acgtcaagct ggaggagctc aaaccagcgt 8641 cgtgggggat tgcggagtgg tcggaggcca ttgagccttt ctctgctgtt ttgtacacat 8701 cagcgcaagt gaacaaggat gaaataagcg tcattcattc cgcgtgcaga gagaggaaga 8761 aagtatttgt tcctgccgtt tgtctgatgc aaaaaggaat agccggaccg ttgattcatc 8821 ccgaatcaga agcttgctta gaatcggctt ggcgtcgttt gcaccgacag accttgtgtg 8881 cggaccccga tcaacatcca ttctcgatga cggcagcagc gatgctagcg aacgtcgctg 8941 tgttcgaatg gttcaaacat gttactggcg tcaatgacaa tgaggatgaa aacaaggtgt 9001 atgtactgaa tctggagaca ttggaaggaa gctggcgatc attcctgccc catccgatgg 9061 tgaaggaagg aatgaggctg gaggtattgg atagaaagga gcttgaatca cggctatcca 9121 atgagacgga gccaaaggaa ggactacagt ctctgtttca gtcgtggacc tcatcggata 9181 ccggaatttt tcacagttgg gatgagggcg agctcacgca attaccgctc acgcagtgta 9241 atgttacagc agtcgatcct gtctcagaag gcccggcgga gcttatgccg caaatcgtct 9301 gcccaggtta tacccatgag gaggcacgaa gagaagcggg actgcttggg gcagaaatgt 9361 acgttggaag aatggtgaac cacctcgcag ccatagattc agcttttcga aatgtaggaa 9421 atggcaatat cggagtcgga gcaggggaaa cggcagcaga aggacttaat cgtgcactgc 9481 ggcattgctt gacgcttacg tatgcagcag acatgaaagg gagacagcca acagcgagac 9541 ctgtccacac tgacacaatt gctgatgaaa gatgccaatt ttatatccgg tcatttacta 9601 cgttgggtga aaaaccaacg attgcaaagg ggcatgaggt gttcggattt tcggtgtatt 9661 gggtggaaac gagcacggga tggtacggga gtattggctt gcaggagcga gaggcactgc 9721 gatcagcgct gatgtatgcc ttgcaacaga ttcagacccc aaccaatcaa atgtccaagt 9781 atgtgttgac agcgcaatct gttgggttga cgggtgaagc catcgaagtt gcacgcaaca 9841 gcgttcagaa agaatcggaa aaagaggtct ggaaacaagc tgtcgaggtg ctggcccttc 9901 atcacctttc tcctcgggta atgaatgccg cgatggagcc tttcctggaa gaagaccttg 9961 gtggcgtatt cgccttgctg ctctcgaggg aggtgcctcg atgagcgctg acattcttgt 10021 catcggagac ggtgttttgg cagagctggt ttcagaacgt ttgaggaagg actatcgcgt 10081 gtggcacagt gaaaacatgg agtctgcgac aagcggagca gatttggctc tcctgttaca 10141 agatacgtac catcccgctt tgcatttgca ggcagaaaaa cattttcgtc aaacaaatat 10201 cccgtggctt cgcggctttg tttcatttgg ggaggcaatc gtaggtccgt tggttcttcc 10261 aggtcaaaca gggtgctccc agtgtgcaga tatacggcga ctgatggcgg gacgcgatcg 10321 caaagaaatg tggaaggtac aacacatgtt cagcgaaaaa ggcggcatac cgcgtgaccc 10381 gtggtcatct cgctctgggt tgttacaggt ggcatttcat gtgagtgcgg aggttgaacg 10441 catagttgag ggcaggcaag cccagttgtc agagcatatc caattcattg atttacaaac 10501 attgacaagc acacgccact tctttttgcc gaatccgtta tgtccggtat gcagtcggct 10561 gcctgaggat tctgaagagc aggctagagt acgcttgaag cctattgtaa agcaaagtcc 10621 ggacagctac cggacaaaac ccatagccga tttgaaacaa gctctcccca ccgaattttt 10681 ggattcccgt actggatttt tgaatgggaa agtacgtgat cttgtctctg tgttcgcaga 10741 tgtaagtgtc aatcttccgt tattttcaca tgacgagggt acggccggaa ggtctgtttc 10801 gtacgcggat agtgagataa cagcgatttt agaaggaatg gaaagatatt gtgggctgat 10861 gccgcgtggc aagaggacga tggtgcgcga ctgctatgca aaccttgcgc cgcatgcact 10921 tgatccgacg acagcgggaa cccattcaga agagcagtat gccctgcctg attttccgtt 10981 tcagccgttt gatcccaagc aagatatgaa ctgggtatgg ggttattcct ttaaagaaga 11041 gcgacccatt ctagtacctg agctcatggc ctactacagt atgggattcg gggagggagt 11101 tgtatttgag acctccaatg gctgtgcgtt aggtgggagt ttggaggagg cgatctttta 11161 cggcatcctc gaagtggtgg agcgagatgc tttcttgatg acctggtatg ccaaattgcc 11221 attgccgcgc ctcgatccgg cttcggtcaa cgatcgggaa ttgcttctgt tgatcgatcg 11281 gatgcagacc gttaccaatt acgatttgta cttgttcgat gcgacaatgg aaaatggaat 11341 tcctgctgta tggacgattg cgaaaaaccg cggagaggca ggcttgcacc tcgtttgtgc 11401 agcaggagcc catccagacc ccatccgcgc tgtaaaaagc tctattttcg aaacggctgc 11461 catgctcatg cctttgacag acaagtacga agcgaatcga gacaaatatt tgcgtatgct 11521 ccatgattca tccttggtga aaaagatgga cgatcatgcg atgctttaca gcttgccgga 11581 agcagcggag cgcttatcct ttcttatgga tgattcccgc ccgatggtct cattcgctga 11641 acaatttcct aaaagaaagc agcatcgtga tctaacagac gatttgcggg agttgctcgg 11701 tgtgttccaa aaattggatc tcaatgtcat tgtggtggat caaacgacac cagaagtcga 11761 gcgccacggc ttatcttgcg tgcgtgtatt gattcctggc atgctcccga tgacttttgg 11821 ccatcatctc acgcgagtaa ccggattgaa aagagcattg gaggttcctg taaagctggg 11881 ttacgcgaaa caaccactgc gtgtagaaga gttgaatcct tttccgcatc cgtttccata 11941 acatgcatct gggagtgagc cggatgagtc tcgaaaattt tttgtaccgc cttcattttg 12001 atacagaaaa cgcccgacca agtgatctcg aagtggattg ggaggatgcg ccactgacat 12061 acaagctgta caggggacta cctgtcattc cactgccaga ggacgtgcct ctatcgctca 12121 tagatcaaac ggtttcacaa gagatgtctc ttcctcaatt gggtgcttgg ttatggtata 12181 gctacggact catccaaatc agccaatcgt tgttaaccac gattccggga gatgcaggat 12241 ctgagtcgtt tttgccgctc gtaacgaatc gaagaccggt tccgtctggg ggagggctct 12301 atccgagtga gctgtacgtg tatttgaagt tgtcgagtat tcctgcaggt ctctaccact 12361 acgatgtggc tcatcacaga ctcgtgcgat tgcgggaagg gaattttgat gacgtcctta 12421 gccgggcgct tggtggccat tgtccggtac atgcatgctt tggagttgcg ttcgtatcca 12481 tctatttttg gaagaacttt ttcaagtatg atgagttttc ctatcgattg cagggccttg 12541 atgctggtgt actgctcggg caattgcagg aaatggcaaa ccagctcggt gtgacgaatg 12601 cggtacatta ccagttccta gatagagcga tgaatgcatt gctgggattg tctgttgatc 12661 aagagagtgt atacgcggta gtccccttat cgcttcaaac agaaggagag aggcattcgc 12721 actcacccgc acattgggca gagcgaggag aggagacggc aagcgagcta atccgagaac 12781 tgcctgagca aaagcatgtg catgttatgc gttcaaaaag aatgagacaa tatcctgtgc 12841 tcttgcggat gaatgaagct gccatgcagg aatctcccca ttcgtttaca agttattcgg 12901 atcacttgga aaacaagtgg actcgtcgtt ccgaagagcg aatccttctc ccacggttgg 12961 agaaaccaag ctatgatctg gcgcaatttt cacgggagcg tttttcaccg gaaatggact 13021 ttatcaggag gaaaacaggc cgagatcagg tagcgcgtat catggaaatg gcgatgagtg 13081 ttctatcgta tggcaatgat ttgggaaaaa atcaggaagt agcgtggaga aggctttctc 13141 tccttgtaag cattcatcag gtagaaggga tacaagatgg ggcatacgct tacgaaccac 13201 tggagcacgc tctttatccg attgtctatg gagatttacg cgcacgcctt caagacggaa 13261 tgaccctgga caatgtgaat ttgtatcaag tgccgctctg ctttcatgtg gcgggtaaac 13321 gcgatcatct gatcaatcag cttgggttta gagggtatcg catccagcag atggaggcgg 13381 gaatcgtcgt gcatcgcttg cttttggcag cgttcacgtg tggaatgggc ggtcatccgc 13441 tactcggatt tgacgtaaaa aattgcgatg atctttacaa cctagagaca gaagagaaaa 13501 cctgcttgat tcaaattccg gtcgggcatt atcgcccccg cgccaggttt cagggcgggc 13561 ttcatggata aatacgggga agacccgagt caacgctgac ccgggtcttg tattatgtcc 13621 ttatgatggc tttgatacct tgaatgaagc ggtcaatttc ctcgtgggta ttgtacggtg 13681 ccagccccgc tcgtatccag ccgccacttt cattgattcc taaacaattg gcgagtgttg 13741 tcgcatagaa gtccccgtcc gcaacgaata tgctatgctc ctcgcacaat cgctcacata 13801 tttctcgggg actaaaccct tcgatccgaa aagcgaccgt cggtgttttc ggtacatctt 13861 cacccgcttg atagagtgtg acgcccttca ttgcgcttag ttcttttctc agcttatctg 13921 ccagatgatt ttcataggct tcgatcatct cgtaaccaga gcggattttt tcagcaaagc 13981 tatcaccgaa accgagcgaa gcaacaaatt cgatagcggg tctgattcct gcgattcctt 14041 catgattttg tgttccggtc tccagtttat ctggataaga agagggggca ggaaccaatt 14101 tgtaaacatc gagtgcttca aaaatttctt tgcggatgac agcgattccc acatgaggac 14161 caaaaaactt gtaggctgaa cagagcagga tatcggctcc aatctcgtca cgatggatgg 14221 agaagtgagg gaccgcatgc acggcgtcga cggcgaaaat gactcctttg gcacgtgctt 14281 tttgggaaat ggcagcgaga tcatggatcg ttccgacagc attggatgca agcccgactg 14341 ccacaagtct ggttctggaa gtaaggaggg tatcgagctc atctagctgt aacgtaagtg 14401 tctctttatt cacctttaac caccgaacgg tcattccgcg atcagcggca acagtcagcc 14461 acgggtctac attggcgcgg tgatccatct cggagacgat gatctcatcc ccttctttcc 14521 aatgccgacc tagtgcacgc gcaatcgcaa aggtcagcgt ggtcatattt gcaccgaagg 14581 ctacctcctt cggcctcacg tggaagaggt ctgctacggc ttgcttggca tctgcaagga 14641 tggcttctgt ctccttgctc gttggaaagg agccgtgtaa attcgctcca ccgttcgcca 14701 taaaacgata gatcgcgtcc atagaggatt ttacgacttg cgagccgcct gggccatcca 14761 gataaacaac tggtttgcct tcgtaggttc gttgtagtgc ggggaattgc tcacggactt 14821 tttcaatggg gtaatgttcc gatgcaactt ctaatcgcca atcattggaa cgcataaaac 14881 ttccgggagg gtattcaaag acgcattcag actgcagagt gaaaccgagc ttacggcaaa 14941 tggcattgga ggcagggttg tctacagagg gataggcatg aataaacttg tgtcttcgtt 15001 cttggttgat gcgtgtgatg agtgaggcta aagccgcaga ggcgattcct ttcccttgaa 15061 agggtggcag gacgctccag cctacttcgt atacgtgttc tccttgccag atgcggtccc 15121 aatagccgat gcttccgaca gggatgtgat ctggcagcag tacgatgctg aacatcttgc 15181 tggattgctg accgtcctta ttgacgtatc gctcgtgccg ggcgaggagt tgctcctctg 15241 tctctggacc tccaacatgc tccatcatgg caggcgcatt caaaagatgc agcaagggaa 15301 gatcgtgatc tgcccagggc tgaatacgaa caagctcagt gttaagcgtt gtggacattt 15361 tctcactcct tatcaactgg atattcgtgg tgtccaacat cattcggtat ctctcattat 15421 tcggaaaaga ttccaattaa tcaatctttc attcgtttga gaaagaggga tggaaaatca 15481 tgctataatg ttagatgccc gagagattca ctgagaggaa ggggtacctg cccatgaatg 15541 aaaaaagcat ccatacacaa cggcttatat tgattccatt tacacataga atagcgacga 15601 atattttgca agaaaattac gaagagttat tggatatggg gcttacatta ggcaaaggct 15661 ggcctgatga ggatgcgatg gaaacgatac ccaaaatcat caaagcactc gaactcgcag 15721 gggaaccaac aggatttgaa tcgtggatga tcattacaaa agatggtagg aagatcatcg 15781 gtgatgcagg gttcaaggga gccccgaata cagagggaga agtggacatc ggctatggaa 15841 ttattgaagc cgaacgaaaa aaaggctatg cgtacgaggc ggcagaaggc ttggcgaatt 15901 gggcgttgtt gcagccagat gtgaaaaaga ttacggcgaa gtgcttgcta gataatatag 15961 attccgcaaa attattagtc aaaatgggct tcacagaaat aaagagggac gacaccatga 16021 tttactggtc tagtcagaag ggataatagt ctacatgaaa attttcaatg gaagggtaac 16081 ccaagacctc cttgtttcgt attgaaataa ggaaaagatt atcgcttttc attgaactat 16141 gcaacgcgtg tctctatact gaagagataa gcattggtaa ataattgtga gggataacat 16201 gcagaactgg atgacccaaa ttattgaaca atatagttac ttcggtatct tactcatgat 16261 ggcactggaa aacatctttc cacccatacc ttctgaggtg atcttgacct tcggtggatt 16321 tatgacgacg caatcatcct taacggtttt tggcgtaatc ttgtcagcta caatcggttc 16381 ggtgatcggg gcaatcatgc tctatggaat cggtttctac ctagacgtcc aaaaaataga 16441 gaagatcgta gagagatggg ggcatcttct gcgcattaaa acggcagata tccataaagc 16501 gaatgaatgg ttcgaccgtt acggatactg gacgatcttt ttttgtcgca tgatcccatt 16561 aatcagaagc ctgatttcca ttccagctgg tatgaccaaa atgaatttcc cgctgttttt 16621 gctgtttaca acactcggta cgtttatttg gaacatcgtc ctagtgatgg ctggtcattt 16681 actgggggaa tcgtgggaag acattctgta ctatttcgat ctctattcca atgtcgttta 16741 tgttgtactc gcttttacgg gggtagcctt tctcattttt ttcctccgca aacgaaaagc 16801 ggatgcctaa aaccttccaa accatctttc cttcaaggga aggtggtttt ccatttggaa 16861 ccaacaggta aacatattat aaaaagttgt aattttcttg caaatacaag gtgtcgaaat 16921 tgcggtaagg tgtagggagg ggagtaggtg acatgggcac cgtccttatt cttgaagctg 16981 ggggagatgc aacttgatcg atttgttact cgtggacggc gtagtcatta ccatggacaa 17041 agaccgtcgg gtattacaag acggggcagt cgccatagat aaaggaagaa ttttggaggt 17101 aggcgacact ccacttctta aagacaagta tcctgcacaa aaggtagtcg actgcgcgca 17161 tcattgtata ctgccaggct tcgttgacgc acatggtcat ggcggacatt cgctgttcaa 17221 aacgattgcg acagacaata tcaacgactg gatgccaatc atgaccaaca cttacaagca 17281 ctacgtgaca gacgatttct ggtactacga gggaaagctg tcagcactgg agcggcttaa 17341 agctggaatt acgacaggcg ttagcgtact aggctcgatg ccgcgctcag atgatccgat 17401 ctttgccttg aatcacgcca aagcgtataa cgaggttggc gttcgtgaaa tcgtagcgac 17461 aggtccttgc aatccgccgt ggccgcatcc ttttagccga tgggtcgatg gacaaaaagt 17521 aacgaaggaa gtcacgtatg agcaggtgct tgctggagca gaagcagtga ttgaagccct 17581 gaatcacgca aataacgata aaaccagagc atacataacg ccatttgtca tcgtcacctc 17641 tgtcaatccg tcctatccga caccagctga ccaattgttt ggcttaaccg attttgaccg 17701 ttaccaagcg cggaaaatca gggagattgc aaaaaagtac gacacgcgca tccattccga 17761 tgcttttgga ggcatgattc acctcgcgat tcaagacaaa gagtacgcgc tgctcggtcc 17821 agacgtgcac ttgcagcatt gtcggggaat ttcttttgac gaggcgaaaa ttttggccga 17881 aacaggaacg aatgtgagtg cttctcctgg ttttggtcaa atcaatgcac gcactcccat 17941 tacggaattg ctggagatgg gagcaactgt tgcgatcacg acggacggca cctcgccgat 18001 gactcctttt gacatgttcc aggccatgcg cagaatgcag ctgctgcaac aagcggcgct 18061 gcgtgattat tactacctcc cgccgggcaa gctgctggaa atggtcacga ttgatgcggc 18121 acgctgtgtc ggctgggatg acgaactggg gtcactggaa gctggtaaaa aagcggatgt 18181 cattaccgta aatatgcatc aggcccactt gaatccggag aacatgcatg tccatcgtgt 18241 cgtctatcag gcagttggtg gagatgtgaa ccatgtcatt gtcgatggtg agctgattat 18301 ggacgagcgc cgtgttttga ctgtggatga aaagcaaatc ctcaaagaag cgaatgaaga 18361 ggcgaaccgc acaatcgagc gggcaggctt agaaatctac atgcagccaa gcaagtattt 18421 ctgggggcat gcgcgagctt atttggatga gagaagattt gatccgacga agttgtaggg 18481 gaaaaagagt ggataaacga agacccatgt aatgctgcac aaaaaactag gttaccgcat 18541 tgaataggag aaggtgcccg ccgccttccc ttttttatgg attatagttg acaaaaacga 18601 taggattaat cattatttat tcataatccc catcgacaaa gttggaataa aaagaggtgc 18661 agtcatgtat ctgactattg ataatgttac gaaacatttt gagaatgagc agaaacaaaa 18721 agtaaaggtg ctggatgata ttaatcttga agtggaaaag ggcagcttcg tttccattgt 18781 cggcccctct ggctgtggta aatcgacgct gctctactta atagccggat tggatcgacc 18841 agacagcggt gacatacacg ttgctggcaa caaagtgaat aagcccggtc ccgacagagt 18901 cgtcgttttc caagaagcgg gattatttcc atggctaact gttttggaaa atgttacata 18961 cgggctcaag ttgaagaaaa tgtctgcagc gaaagccaac gaaaaggcgc tggaagtgct 19021 caaaatggtc catttaagca agtatgttca ttcctatccc catcagctct caggcggaat 19081 gaagcaacgt gtagccatag ctcgggcact tgtcatggaa cccgatattt tgctgatgga 19141 tgaaccgttc tcggcgctgg atgaacagac gaggatggtt cttcataagg agttgcttga 19201 gatttggcgg acgaccaagg tcacgatttt tttcgtgacc cataatatcc gagaggccgt 19261 tcagcttggg gagaagatcg tcgtttttgc cacacgcccc ggcaaaatca aggagatcat 19321 tgcagttcct gccatgcgcg atggagtcat gccggacagt gtgacattaa gcaccgagca 19381 gaaggtgctg tcgatcctgc aagaagagat agaaaaggtg ctgaaggagg aaatgggcaa 19441 tgactacagc tttaagacgg gtcatcttca tcgctatgat agcggcgatc tgggaagtca 19501 catctagatt atctgggctc ccatcgttca tgtttcctag cttgacccag gttttcgaaa 19561 cactcgtaaa tggcttgata agcgggcaaa ttaccgctgc gattggaaaa agcatgggac 19621 gaatcctgtt gggctttctc attgccatta tcgtcggact gatattgggt tacttcattt 19681 ggaggtacaa actcgtagaa gatacgcttg gctttgtggt gacagcgctt cagtctatcc 19741 cgagcattgt ctggttcccg cttgcgatta tctggtttgg cttaaatgac ttctccattt 19801 tgttcatcgt gacgatcggg gcgacctgga cgatgacggt caatgctacc agcggtttca 19861 aaaatgtccc gccgctatat caacgagtcg ccaagacata cggttcgtct ggctttcact 19921 ttgtacgaac agtcattctt cctgcttccg taccgcagat catctccggg cttcgaatcg 19981 cttgggcatt ttcctggcgg gcacttatgg ctggtgaatt gctcgggggt ggcggtggtc 20041 tcggtcaatt gctcgagatg ggacgttcac ttggacaaat ggatttggtg atatcagtga 20101 tgatcattat tgcgatcatc ggtaccatcg tcgataatgt tgtcttttca cgcatcgaac 20161 gcaacgttca gatgaagtgg ggagtccatt cataaaagaa aaatggccta aggagtgtgt 20221 cgtcacatgc ttaaaaaagt gctcagtacc gttgtcgcat ccctattgtt gatgggggtt 20281 gcgagcgggt gctctactgg aggagcagaa tcctctgaga aggaagtaag gatcggatat 20341 ttcccaaatc tgacccatag cgcaacgatt atcgcgctgg agaaaggcta tttcaaggaa 20401 gcttttggtg cggatgtaaa gattcaaaca aaaacggtca ccaatggcgg attgtttatg 20461 gaagcgatgg caaccaaagc aattgatgta gggacagtcg gacctggacc gctgttgaac 20521 ttttatgtga agcaccccgg gtatcgcttg atttcagggg cggtcaatgg cggagctgtg 20581 ctcgtcatga atggttcaac caatattacg gaactaaaag atttaaaagg caaaaaaatc 20641 gcgattccgg ttatcggcag cacacaggat gtcatgctta gaaaagcatt gaatgaggtt 20701 ggtctcaaac cgacgacgaa tggtggagat gtggaactgt atgctgcggc tccagcagat 20761 acggcagcat tattcgtgca aaaatccgtt gatggtgcag caactcaaga gccatgggga 20821 tatgtactgg aaaatcaggc agaaggaaaa ttgcttttgg actgggatca attcgcttgg 20881 gggaaagaat caaccaatac tgtcgtagca gccagcgatg aatttttgaa acgagaagga 20941 ttggcaaccg cctacctgca agcacataaa aaggcagtca aattcattca ggaaaatccc 21001 gaagaaagcc aagacctgat catcaagcat ttgaaaggtc tgactggcaa agaactgagc 21061 aaaaaagaag tgcaggcagc cttttcccgt ttggaagtaa caacggcggt caatgaaaaa 21121 gtcattcagg aaatggccga tatcagccag gaagccggct atatttccag taataagatc 21181 gatggtttga ttgatttaaa atatttggaa gcggtgaaat aatatcgagt gagaaggagc 21241 gagattagca ggaggaacgg gggatggaaa ggaattagat gggaaaggag gtcatgccgc 21301 gatgagacca catgaacaag gaagtaatca accaaacgac acttcattgg agacgcagat 21361 tcatactgat ttccaaaaag agatgacgta cggcgattat ttacagctcg atcaaatctt 21421 gtcgagtcaa caatgccaat ccagccatca tgatgaaatg ctgtttatta tcattcatca 21481 ggtgagtgag ctatggatga aacaaatttt gcatgaactg tcagcagcaa atgagtgcat 21541 ctgcaatcac gatctggagc ctgctttcaa aatgttcgcg cgagtctcgc gtatccagca 21601 gcaattgatt aaatcgtggg atgtcctttc tacattgacg ccagcagact acctccagtt 21661 ccgtgacaag ctgggtcatt cgtccggttt tcaatcctat caaaaccgac tcatcgaatt 21721 tacgctaggt tataagaacc agcatgtcct ggcggtgtat gcgcatcaac cggagcttca 21781 cgcccaaatg agtgcggcct tgcagcagcc tagcatttac gatgcggcga tcagggagat 21841 ggctgctcgt ggcttgccga ttgatccgga atgcttgaat cgcgattggt cacagccata 21901 ccaacccaat cagagtgtag agcaagcctg gctgaccgtg tatcgaaatg tgaaccagta 21961 ttgggatctg tacgagttgg cagaaaagct ggtggatatc gccagtcagc aacagcaatg 22021 gcgttttaac catatgacga cagtcgaacg catcataggt caaaagccgg gaacaggagg 22081 gtcatcaggc gtcatgtact tgcgtagagc gctcgatcac cgttttttcc cggagctgtg 22141 gagcttgcgg actctgttgt agaaaaattc atcaaaaaaa gcgagaaaag cgtcttgggg 22201 acccagggcg ctttttttcg tgtagtactc atttcacatc ttttcacata tccttcctaa 22261 tattgggtac aaataccctt cttttcctga caaacatatg ttttcatttt tactagtata 22321 catatttttc aagaacaaca agaaatggag tgggcacttg gatggaaaca atgaagccac 22381 ctatggaagt gttatatgcc aaacagctga atgcactgcg atcccatgat acagggacaa 22441 aacctcccaa ttggctgatg tctcccagag ctgtgcgcga ttttatttta ggaacagatg 22501 aacccctctc ttatgagggc gaaagtataa cgattacgaa gaagttttat ggagacgatg 22561 tgcttattga gcgtgcaatc gtcacgttgg caggcaatcg cggcttgatg ctcgtcggtg 22621 aacctggaac ggcaaagacg atgctgtctg agctgctgtc tgcggccatc tccggaacga 22681 gcacgaatac gatccaaggc accgcaggaa cgactgagga tatgatcaaa tactcctgga 22741 actacgcgat gctgctggac aaaggtcctt cgctgcaagc gctggttcct tccccgttat 22801 acaccggaat gagcaaggga atcattaccc gttttgaaga aattacccgt tgcccgtttg 22861 aggtccaaga cgtgttgatt agtattttga gcgacaaggt gatgaacatc cccgagctgt 22921 cagacggcat cctgtttgcg aagcccggtt ttaatatcat cgcaacagcg aatctgcggg 22981 acaaaggcgt caatgaaatg agcagcgcgc tcaaacgccg ttttaacttc gagacaatcg 23041 cgccgattaa tcatgtgaaa atggaagcac aaattatcga atcccaagca aaagtgattc 23101 tggagcaaag tggcgtaaac atcgaaatcg atcgggatgt ggttgagata ttggcgacga 23161 ccttcatgga actgcgcatg ggcgagacca aggaaggttt caaaatcgat tccccccaat 23221 ccgtgatgag tacagccgaa gccgtatccg tctatgtcca aagtgcgatg acgtcccatt 23281 actatgatgg caggcccatt tctatggatc gattggtgca gaacatgctg ggagcggtcg 23341 tcaaggaaaa ccagaaggac gcaaatatcc tgaaaaccta ctttaccaaa gtggtcaaag 23401 aacgggcaaa ggaagagggt ctatggggaa cgtattacaa cgagaagaaa tggatcaaat 23461 aaacagtcag gaccgcttca taaagtcatt cgtcgaagaa gaggtctaca acctcagcaa 23521 acaggttgtc tatttcccgg ttcgtcatca tagtcctgcc tgcgcgtttc a //