GCF_006402015.1 - 27 region(s)

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Location: 1,042,767 - 1,070,712 nt. (total: 27,946 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BHS07_RS04620: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

BHS07_RS04630: Halogenation

MITE: MITE0000024Halogenation (identity: 67.70%)
smcogs: SMCOG1222: dehydrogenase

BHS07_RS04695: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: dehydrogenase

BHS07_RS04630: Halogenation

MITE: MITE0000024Halogenation (identity: 67.70%)
smcogs: SMCOG1222: dehydrogenase

Hydrolases: esterase

BHS07_RS04600: enterobactin/ferric enterobactin esterase

smcogs: SMCOG1098: enterobactin/ferric enterobactin esterase

Lyases: dehydratase

BHS07_RS04605: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthetase

BHS07_RS04615: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Translocases: permease

BHS07_RS04680: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000648.3	0.92	terpene	carotenoid	Myxococcus xanthus
BGC0000647.3	0.76	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.58	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.55	terpene	isorenieratene	Streptomyces argillaceus
BGC0001227.3	0.55	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000644.3	0.53	terpene	carotenoid	Dietzia sp. CQ4
BGC0000633.3	0.53	terpene	carotenoid	Streptomyces avermitilis
BGC0000649.3	0.53	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002276.2	0.52	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000656.5	0.51	terpene	zeaxanthin	Xanthobacter autotrophicus Py2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000648.3	0.92	terpene	carotenoid	Myxococcus xanthus
BGC0000647.3	0.76	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.58	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.55	terpene	isorenieratene	Streptomyces argillaceus
BGC0001227.3	0.55	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000644.3	0.53	terpene	carotenoid	Dietzia sp. CQ4
BGC0000633.3	0.53	terpene	carotenoid	Streptomyces avermitilis
BGC0000649.3	0.53	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002276.2	0.52	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000656.5	0.51	terpene	zeaxanthin	Xanthobacter autotrophicus Py2

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

BHS07_RS04615: PT_FPPS_like

Domain 1 (57 - 359): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

BHS07_RS04625: PT_phytoene_like

Domain 1 (13 - 315): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → diapophytoene; FPP → squalene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

BHS07_RS04645: Lycopene_cycl

Domain 1 (1 - 91): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

BHS07_RS04650: Lycopene_cycl

Domain 1 (6 - 99): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.57 out of 26.12

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.56 out of 29.65

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.41 out of 29.25

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description

NZ_CP017172.1 - Region 2 - NRPS-like

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Location: 1,743,825 - 1,788,237 nt. (total: 44,413 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BHS07_RS07535: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: transferase

BHS07_RS07555: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: glucanase

BHS07_RS07600: Pullulanase, type I

smcogs: SMCOG1297: Pullulanase, type I

Lyases: cyclase dehydratase

BHS07_RS07560: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

BHS07_RS07565: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

BHS07_RS07585: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase

BHS07_RS07570: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000871.4	0.94	other (nucleoside)	VEPE, AEPE, TG-1	Myxococcus xanthus DK 1622
BGC0001641.4	0.58	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001285.5	0.47	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0002474.2	0.46	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0001168.5	0.44	NRPS	Livipeptin	Streptomyces lividans 1326
BGC0000898.5	0.43	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000826.5	0.43	other (other)	staurosporine	Streptomyces clavuligerus ATCC 27064
BGC0002711.2	0.43	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0000597.4	0.41	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0000243.4	0.41	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000871.4	0.94	other (nucleoside)	VEPE, AEPE, TG-1	Myxococcus xanthus DK 1622
BGC0001641.4	0.58	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001285.5	0.47	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0002474.2	0.46	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0001168.5	0.44	NRPS	Livipeptin	Streptomyces lividans 1326
BGC0000898.5	0.43	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000826.5	0.43	other (other)	staurosporine	Streptomyces clavuligerus ATCC 27064
BGC0002711.2	0.43	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0000597.4	0.41	ribosomal	SWA-2138	Streptomyces sp. e14
BGC0000243.4	0.41	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.85 out of 29.72

Confidence: medium

Additional weak hits (7):

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.98 out of 20.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.72 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.24 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.00 out of 18.57

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.81 out of 21.84

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.91 out of 32.74

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.09 out of 30.98

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 1743824 - 1788237:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS07570: X

AMP-binding (568..998): X

nrpys: Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LKLLFEFAAG-TVG--------------ALMATH
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
NtrC	Nitrogen regulatory protein C

NZ_CP017172.1 - Region 3 - NRPS,NRPS-like

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Location: 1,838,201 - 1,906,176 nt. (total: 67,976 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

BHS07_RS07920: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

BHS07_RS07955: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acyltransferase methyltransferase

BHS07_RS07930: acyltransferase

smcogs: SMCOG1141: acyltransferase

BHS07_RS07945: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

BHS07_RS07950: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Lyases: dehydratase

BHS07_RS07925: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: isomerase

BHS07_RS07900: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

BHS07_RS07895: carbamoyl phosphate synthase small subunit

smcogs: SMCOG1249: carbamoyl phosphate synthase small subunit

BHS07_RS07975: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002274.2	0.56	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002164.2	0.51	NRPS	peramine	Epichloe festucae
BGC0001160.5	0.44	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.44	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001833.4	0.43	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.42	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.42	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.41	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.41	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.74	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001641.4	0.69	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.75 out of 23.79

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.64 out of 23.79

Confidence: medium

Additional weak hits (5):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.98 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.87 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.03 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.33 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.12 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 1838200 - 1906176:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Acetyl-CoA) + (X - Lys - X - Leu - Leu - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 1838200 - 1882118:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Acetyl-CoA) + (X - Lys - X - Leu - Leu - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 1846307 - 1906176:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Acetyl-CoA) + (X - Lys - X - Leu - Leu - X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS07955: Acetyl-CoA

CAL_domain (208..612): Acetyl-CoA

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 27.4
AHBA: 11.2
NH2: 7.1
shikimic_acid: 0.0
fatty_acid: 0.0

BHS07_RS07975: X - Lys - X - Leu - Leu - X

Search NORINE for peptide: strict or relaxed

AMP-binding (516..904): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ASFSFDMHLEDFVIVVGGEINAYGPTETTICVVA
Nearest Stachelhaus code(s):: Leu DAMLVGAICK (56% 8Å match); Arg DAEGVGAIAK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1564..1952): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ASFSFDIHLEEYSTISGGELNTYGPTETTICVVA
Nearest Stachelhaus code(s):: N6-ohLys or Lys DIESIGTIVK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (2612..3006): X

nrpys: Gln, Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LALAFDASVQQVDCLVGGEFNVYGPTECTVDVTA
Nearest Stachelhaus code(s):: Gln or Glu DAQDLGVVDK (88% 8Å match); Ile DAQDLGVVDK (85% 8Å match); Gln DAQDLGVVDK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3666..4054): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAAFDVHVLEYLAQLGGELNGYGPCEATCLSSL
Nearest Stachelhaus code(s):: Pro DVLLVAGVLK (59% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (4712..5100): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAAFDVHVLEYLAQLGGELNGYGPCEATCLSSL
Nearest Stachelhaus code(s):: Pro DVLLVAGVLK (59% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (5757..6161): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HAMAFDAAAEDPSLAPGGDLNVYGPTECTITSAT
Nearest Stachelhaus code(s):: Arg DAEDVGVIGK (62% 8Å match); Ahp DVENAGVVTK (59% 8Å match); Lys DAESVGTIIK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_CP017172.1 - Region 4 - thioamitides

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Location: 2,720,801 - 2,743,130 nt. (total: 22,330 nt) Show pHMM detection rules used

thioamitides: ((YcaO or TIGR03604) and TfuA)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: reductase

BHS07_RS11635: alkyl hydroperoxide reductase/ Thiol specific

smcogs: SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001849.4	0.48	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0000625.5	0.36	ribosomal	thioviridamide	Streptomyces olivoviridis
BGC0002339.3	0.36	terpene	2-hydroxysorangiadenosine	Vitiosangium cumulatum
BGC0002120.3	0.31	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0001430.4	0.29	NRPS, PKS	myxochromide S	Stigmatella aurantiaca Sg a15
BGC0002326.2	0.29	NRPS	endopyrrole B, endopyrrole C, endopyrrole A	Mycetohabitans rhizoxinica
BGC0001053.5	0.28	NRPS, PKS	tubulysin A	Archangium disciforme
BGC0001127.5	0.27	NRPS	jagaricin	Janthinobacterium agaricidamnosum
BGC0001964.2	0.26	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001473.4	0.25	ribosomal	Sch 18640	Micromonospora arborensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001849.4	0.48	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0000625.5	0.36	ribosomal	thioviridamide	Streptomyces olivoviridis
BGC0002339.3	0.36	terpene	2-hydroxysorangiadenosine	Vitiosangium cumulatum
BGC0002120.3	0.31	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0001430.4	0.29	NRPS, PKS	myxochromide S	Stigmatella aurantiaca Sg a15
BGC0002326.2	0.29	NRPS	endopyrrole B, endopyrrole C, endopyrrole A	Mycetohabitans rhizoxinica
BGC0001053.5	0.28	NRPS, PKS	tubulysin A	Archangium disciforme
BGC0001127.5	0.27	NRPS	jagaricin	Janthinobacterium agaricidamnosum
BGC0001964.2	0.26	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001473.4	0.25	ribosomal	Sch 18640	Micromonospora arborensis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.44 out of 23.79

Confidence: medium

Additional weak hits (5):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.56 out of 26.85

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.89 out of 30.25

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.56 out of 27.04

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.65 out of 24.78

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_CP017172.1 - Region 5 - NRPS-like,T1PKS

Download region SVG

Location: 3,173,755 - 3,225,565 nt. (total: 51,811 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: formyltransferase

BHS07_RS13585: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

Ligases: synthase

BHS07_RS13595: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.60	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.59	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.59	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001273.3	0.59	PKS	asperlactone	Aspergillus ochraceus
BGC0001257.3	0.59	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001244.3	0.59	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.59	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000107.3	0.59	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000107.3	0.63	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.04 out of 32.93

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (15):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.44 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.14 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.28 out of 29.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.22 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.50 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.11 out of 28.14

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 22.71 out of 36.54

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.89 out of 30.25

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.63 out of 30.22

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.47 out of 21.55

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.45 out of 31.73

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.56 out of 29.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.04 out of 28.19

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.23 out of 32.93

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.79 out of 20.01

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 7, location 3173754 - 3225565:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 8, location 3173754 - 3218422:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 9, location 3179301 - 3225565:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS13585: X

A-OX (595..1372): X

No predictions made

BHS07_RS13595: mal

PKS_AT (539..836): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 58.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 146.3
inactive: 85.4
Methoxymalonyl-CoA: 76.6
Methylmalonyl-CoA: 70.4
Isobutyryl-CoA: 68.4
Propionyl-CoA: 50.2
Ethylmalonyl-CoA: 42.9
Benzoyl-CoA: 36.2
fatty_acid: 25.5
CHC-CoA: 20.0
3-Methylbutyryl-CoA: 19.9
Acetyl-CoA: 18.5
trans-1,2-CPDA: 10.9
2-Methylbutyryl-CoA: -1.0

TFBS definitions

Regulator	Description
LexA variant 3	Repressor of DNA damage response
VqsM	Regulator of quorum-sensing signalling systems

NZ_CP017172.1 - Region 6 - lanthipeptide-class-ii

Download region SVG

Location: 3,293,235 - 3,316,552 nt. (total: 23,318 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: amidase

BHS07_RS13985: amidase

smcogs: SMCOG1105: amidase

Lyases: cyclase

BHS07_RS14015: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.57	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001210.5	0.51	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002703.2	0.49	ribosomal	archalan α	Halorussus salinus
BGC0000504.5	0.48	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0001555.4	0.47	ribosomal	colicin V	Escherichia coli chi7122
BGC0002698.2	0.47	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000500.5	0.47	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000590.5	0.47	ribosomal	microcin N	Escherichia coli
BGC0001576.3	0.47	ribosomal	divamide A	Prochloron didemni

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.57	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001210.5	0.51	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002703.2	0.49	ribosomal	archalan α	Halorussus salinus
BGC0000504.5	0.48	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0001555.4	0.47	ribosomal	colicin V	Escherichia coli chi7122
BGC0002698.2	0.47	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000500.5	0.47	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000590.5	0.47	ribosomal	microcin N	Escherichia coli
BGC0001576.3	0.47	ribosomal	divamide A	Prochloron didemni

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BHS07_RS14015

BHS07_RS14000

Leader:

MSQKKEHILRAWRDPEYFNSLSSEERASLPANPAAELELSDEVLENISGA

Core:

DTCEQFGSYYCTPCPPYHCL

Core with dehydrations:

DDhbCysEQFGDhaYYCysDhbPCysPPYHCysL

antiSMASH-DB 4.0 matches for BHS07_RS14000

100.0%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13855_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14000_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13985_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14515_lanthipeptide

Lanthipeptides: BHS04_RS14450_lanthipeptide

1

DTCEQFGSYYCTPCPPYHCL

20

DTCEQFGSYYCTPCPPYHCL

1

DTCEQFGSYYCTPCPPYHCL

20

85.0%

NC_008095.1and 3 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13075_lanthipeptideNZ_CP068048.1 Lanthipeptides: JKA73_RS13970_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35465_lanthipeptide

Lanthipeptides: MXAN_RS13830_lanthipeptide

1

DTCEQFGSYYCTPCPPYHCL

20

D+CEQFGSYYCTPCPPY C+

1

DSCEQFGSYYCTPCPPYVCM

20

80.0%

NC_015711.1and 1 other:

NZ_CP022203.1 Lanthipeptides: MYMAC_RS14145_lanthipeptide

Lanthipeptides: LILAB_RS21660_lanthipeptide

1

DTCEQFGSYYCTPCPPYHCL

20

D+CE+FGSY+CTPCPPY CL

1

DSCERFGSYHCTPCPPYVCL

20

with 12 more sequence matches

Only the first 10 are displayed here.

80.0%

Lanthipeptides: A176_RS20110_lanthipeptide

1

DTCEQFGSYYCTPCPPYHCL

20

D+CEQFGSYYCTPCPP+ C+

1

DSCEQFGSYYCTPCPPFVCM

20

65.0%

Lanthipeptides: A176_RS20105_lanthipeptide

1

DTCEQFGSYYCTPCPPYHC

19

D+CE GS YCTPCPP C

1

DSCETIGSPYCTPCPPIEC

19

52.4%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13860_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14005_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13990_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14520_lanthipeptide

Lanthipeptides: BHS04_RS14455_lanthipeptide

8

SYYCTPCPPYHCL

20

S CTPCPPYHCL

9

SAICTPCPPYHCL

21

50.0%

BGC0001155.5 (AGY49587.1):

Lanthipeptides: A176_RS20100_lanthipeptide

8

SYYCTPCPPYHCL

20

S CTPCPPYHC+

8

SALCTPCPPYHCV

20

47.6%

NC_008095.1and 2 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13080_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35460_lanthipeptide

Lanthipeptides: MXAN_RS13835_lanthipeptide

7

GSYYCTPCPPYHCL

20

S CTPCPP HCL

8

SSAQCTPCPPRHCL

21

47.6%

NZ_CP068048.1:

Lanthipeptides: JKA73_RS13975_lanthipeptide

7

GSYYCTPCPPYHC

19

S YCTPCPP HC

8

SSPYCTPCPPLHC

20

45.0%

NZ_CP022098.1and 2 others:

NZ_CP069396.1 Lanthipeptides: JRI60_RS18040_lanthipeptideNZ_CP069396.1 Lanthipeptides: JRI60_RS18040_lanthipeptide

Lanthipeptides: CYFUS_RS36740_lanthipeptide

8

SYYCTPCPPYHC

19

S YCTPCPP +C

7

SAYCTPCPPKYC

18

42.9%

NZ_CP017169.1and 5 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13865_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14010_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13995_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14530_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14525_lanthipeptide

Lanthipeptides: BHS04_RS14460_lanthipeptide

7

GSYYCTPCPPYHCL

20

S CTPCPP HC

8

SSPQCTPCPPRHCF

21

40.0%

NZ_CP059736.1:

Lanthipeptides: SG35_RS31610_lanthipeptide

10

YCTPCPPYHCL

20

C+PCPP HCL

2

ICSPCPPRHCL

12

36.4%

NZ_CP040916.1:

Lassopeptides: FH965_RS40555_lassopeptide

3

CEQFGSYYCTPCPPYHC

19

E GSYY PPY

1

GEALGSYYEGGFPPYEW

17

MIBiG 4.0 matches for BHS07_RS14000

15.0%

Thiopeptide: GE2270

10

YCTPCPP

16

+C C P

8

FCCSCSP

14

BHS07_RS14005

Leader:

MSQKKDHILRAWRDPEYFNSLTAEERAALPANPAAELDLGDDVLESITGG

Core:

CGCKPGQSSAICTPCPPYHCL

Core with dehydrations:

CysGCysKPGQDhaDhaAICysDhbPCysPPYHCysL

antiSMASH-DB 4.0 matches for BHS07_RS14005

100.0%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13860_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14005_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13990_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14520_lanthipeptide

Lanthipeptides: BHS04_RS14455_lanthipeptide

1

CGCKPGQSSAICTPCPPYHCL

21

CGCKPGQSSAICTPCPPYHCL

1

CGCKPGQSSAICTPCPPYHCL

21

76.2%

NC_008095.1and 2 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13080_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35460_lanthipeptide

Lanthipeptides: MXAN_RS13835_lanthipeptide

3

CKPGQSSAICTPCPPYHCL

21

C PGQSSA CTPCPP HCL

3

CLPGQSSAQCTPCPPRHCL

21

66.7%

NZ_CP017169.1and 5 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13865_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14010_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13995_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14530_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14525_lanthipeptide

Lanthipeptides: BHS04_RS14460_lanthipeptide

3

CKPGQSSAICTPCPPYHCL

21

C PGQSS CTPCPP HC

3

CLPGQSSPQCTPCPPRHCF

21

with 12 more sequence matches

Only the first 10 are displayed here.

61.9%

Lanthipeptides: A176_RS20100_lanthipeptide

5

PGQSSAICTPCPPYHCL

21

P Q SA+CTPCPPYHC+

4

PEQCSALCTPCPPYHCV

20

61.9%

NZ_CP068048.1:

Lanthipeptides: JKA73_RS13975_lanthipeptide

3

CKPGQSSAICTPCPPYHC

20

C GQSS CTPCPP HC

3

CPTGQSSPYCTPCPPLHC

20

52.4%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13855_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14000_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13985_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14515_lanthipeptide

Lanthipeptides: BHS04_RS14450_lanthipeptide

9

SAICTPCPPYHCL

21

S CTPCPPYHCL

8

SYYCTPCPPYHCL

20

47.6%

NC_015711.1and 1 other:

NZ_CP022203.1 Lanthipeptides: MYMAC_RS14145_lanthipeptide

Lanthipeptides: LILAB_RS21660_lanthipeptide

9

SAICTPCPPYHCL

21

S CTPCPPY CL

8

SYHCTPCPPYVCL

20

47.6%

NZ_CP059736.1:

Lanthipeptides: SG35_RS31610_lanthipeptide

10

AICTPCPPYHCL

21

AIC+PCPP HCL

1

AICSPCPPRHCL

12

42.9%

NC_008095.1and 3 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13075_lanthipeptideNZ_CP068048.1 Lanthipeptides: JKA73_RS13970_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35465_lanthipeptide

Lanthipeptides: MXAN_RS13830_lanthipeptide

9

SAICTPCPPYHCL

21

S CTPCPPY C+

8

SYYCTPCPPYVCM

20

42.9%

NZ_CP022098.1and 2 others:

NZ_CP069396.1 Lanthipeptides: JRI60_RS18040_lanthipeptideNZ_CP069396.1 Lanthipeptides: JRI60_RS18040_lanthipeptide

Lanthipeptides: CYFUS_RS36740_lanthipeptide

8

SSAICTPCPPYHC

20

+SA CTPCPP +C

6

TSAYCTPCPPKYC

18

38.1%

Lanthipeptides: A176_RS20105_lanthipeptide

9

SAICTPCPPYHC

20

S CTPCPP C

8

SPYCTPCPPIEC

19

38.1%

BGC0000517.4 (BAB04173.1):

Lanthipeptides: A176_RS20110_lanthipeptide

9

SAICTPCPPYHCL

21

S CTPCPP+ C+

8

SYYCTPCPPFVCM

20

27.8%

NZ_CP059734.1:

Lanthipeptides: SG34_RS31140_lanthipeptide

9

SAICTPCPPYHCL

21

SAIC+PCPP HC

24

SAICSPCPPRHCF

36

MIBiG 4.0 matches for BHS07_RS14005

17.9%

Lanthipeptide: haloduracin β or haloduracin α

3

CKPGQSSAICT

13

C+ G A CT

8

CRLGNKGAYCT

18

17.6%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

2

GCKPGQSSAICTPCPPYHC

20

GCK G HC

10

GCKTGALMGCNMKTATCHC

28

BHS07_RS14010

Leader:

MSQKKDHILRAWRDPEYFNSLSSEERASLPANPAAELELGDDLLEVITGG

Core:

DFCLPGQSSPQCTPCPPRHCF

Core with dehydrations:

DFCysLPGQDhaDhaPQCysDhbPCysPPRHCysF

antiSMASH-DB 4.0 matches for BHS07_RS14010

100.0%

NZ_CP017169.1and 5 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13865_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14010_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13995_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14530_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14525_lanthipeptide

Lanthipeptides: BHS04_RS14460_lanthipeptide

1

DFCLPGQSSPQCTPCPPRHCF

21

DFCLPGQSSPQCTPCPPRHCF

1

DFCLPGQSSPQCTPCPPRHCF

21

90.5%

NC_008095.1and 2 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13080_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35460_lanthipeptide

Lanthipeptides: MXAN_RS13835_lanthipeptide

1

DFCLPGQSSPQCTPCPPRHCF

21

DFCLPGQSS QCTPCPPRHC

1

DFCLPGQSSAQCTPCPPRHCL

21

76.2%

NZ_CP068048.1:

Lanthipeptides: JKA73_RS13975_lanthipeptide

1

DFCLPGQSSPQCTPCPPRHCF

21

DFC GQSSP CTPCPP HC+

1

DFCPTGQSSPYCTPCPPLHCY

21

with 14 more sequence matches

Only the first 10 are displayed here.

66.7%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13860_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14005_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13990_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14520_lanthipeptide

Lanthipeptides: BHS04_RS14455_lanthipeptide

3

CLPGQSSPQCTPCPPRHCF

21

C PGQSS CTPCPP HC

3

CKPGQSSAICTPCPPYHCL

21

52.4%

Lanthipeptides: A176_RS20100_lanthipeptide

5

PGQSSPQCTPCPPRHCF

21

P Q S CTPCPP HC

4

PEQCSALCTPCPPYHCV

20

42.9%

Lanthipeptides: A176_RS20105_lanthipeptide

9

SPQCTPCPPRHCF

21

SP CTPCPP C+

8

SPYCTPCPPIECY

20

42.9%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13855_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14000_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13985_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14515_lanthipeptide

Lanthipeptides: BHS04_RS14450_lanthipeptide

8

SSPQCTPCPPRHCF

21

S CTPCPP HC

7

GSYYCTPCPPYHCL

20

38.1%

NC_008095.1and 3 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13075_lanthipeptideNZ_CP068048.1 Lanthipeptides: JKA73_RS13970_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35465_lanthipeptide

Lanthipeptides: MXAN_RS13830_lanthipeptide

8

SSPQCTPCPPRHCF

21

S CTPCPP C

7

GSYYCTPCPPYVCM

20

38.1%

NC_015711.1and 1 other:

NZ_CP022203.1 Lanthipeptides: MYMAC_RS14145_lanthipeptide

Lanthipeptides: LILAB_RS21660_lanthipeptide

8

SSPQCTPCPPRHCF

21

S CTPCPP C

7

GSYHCTPCPPYVCL

20

38.1%

Lanthipeptides: A176_RS20110_lanthipeptide

8

SSPQCTPCPPRHC

20

S CTPCPP C

7

GSYYCTPCPPFVC

19

38.1%

NZ_CP022098.1and 2 others:

NZ_CP069396.1 Lanthipeptides: JRI60_RS18040_lanthipeptideNZ_CP069396.1 Lanthipeptides: JRI60_RS18040_lanthipeptide

Lanthipeptides: CYFUS_RS36740_lanthipeptide

8

SSPQCTPCPPRHCF

21

+S CTPCPP++C+

6

TSAYCTPCPPKYCY

19

38.1%

NZ_CP059736.1:

Lanthipeptides: SG35_RS31610_lanthipeptide

12

CTPCPPRHCF

21

C+PCPPRHC

3

CSPCPPRHCL

12

31.8%

NC_014718.1:

Lanthipeptides: allorf_507509_507643_lanthipeptide

4

LPGQSSPQCTPCP

16

LP Q P+C CP

1

LPCQQRPRCAACP

13

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.85 out of 23.79

Confidence: medium

Additional weak hits (3):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.90 out of 30.22

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.93 out of 28.19

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

BHS07_RS14000 - Class II: Cleavage pHMM score: -1.40; RODEO score: 0; Monoisotopic mass: 2271.9 Da; Molecular weight: 2273.5 Da; Number of bridges: 3
BHS07_RS14005 - Class II: Cleavage pHMM score: -2.00; RODEO score: 0; Monoisotopic mass: 2109.9 Da; Molecular weight: 2111.5 Da; Number of bridges: 3
BHS07_RS14010 - Class II: Cleavage pHMM score: -2.80; RODEO score: 0; Monoisotopic mass: 2265.0 Da; Molecular weight: 2266.6 Da; Number of bridges: 3

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_CP017172.1 - Region 7 - NRPS-like,T1PKS,T3PKS

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Location: 3,829,511 - 3,896,294 nt. (total: 66,784 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

BHS07_RS16350: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: acylase acyltransferase glycosyltransferase halogenase methyltransferase

BHS07_RS16255: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

BHS07_RS16300: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

BHS07_RS16330: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

BHS07_RS16345: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
smcogs: SMCOG1119: halogenase

BHS07_RS16365: isoprenylcysteine carboxyl methyltransferase

smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

BHS07_RS16375: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BHS07_RS16465: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Lyases: hydratase

BHS07_RS16385: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

BHS07_RS16335: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BHS07_RS16340: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BHS07_RS16370: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

BHS07_RS16380: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001160.5	1.19	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	1.17	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.95	PKS	ACT-Toxin II	Alternaria alternata
BGC0001273.3	0.92	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.91	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.91	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.91	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.90	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0000107.3	0.90	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002154.2	0.90	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001160.5	0.72	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.71	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.65	PKS	ACT-Toxin II	Alternaria alternata
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.64	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001257.3	0.64	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0000107.3	0.64	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002154.2	0.64	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

Additional weak hits (13):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.76 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.96 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.40 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.83 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.34 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.05 out of 28.14

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.85 out of 25.88

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.69 out of 20.97

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.33 out of 25.75

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.31 out of 39.62

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.73 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 11, location 3829510 - 3896294:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Acetyl-CoA) + (ohmal) + (Me-mal)
Non-colinear ordering:: BHS07_RS16340 + BHS07_RS16380 + BHS07_RS16375

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS16340: Acetyl-CoA

CAL_domain (36..483): Acetyl-CoA

minowa: Acetyl-CoA

Prediction, score:

Acetyl-CoA: 22.0
AHBA: 11.8
fatty_acid: 10.9
NH2: 1.0
shikimic_acid: 0.0

BHS07_RS16375: mmal

PKS_AT (572..866): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 75.0%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 141.2
Methoxymalonyl-CoA: 114.5
Ethylmalonyl-CoA: 111.3
Isobutyryl-CoA: 88.7
Malonyl-CoA: 82.6
Propionyl-CoA: 64.9
2-Methylbutyryl-CoA: 55.9
trans-1,2-CPDA: 52.0
Benzoyl-CoA: 50.3
CHC-CoA: 44.4
Acetyl-CoA: 40.0
fatty_acid: 36.8
3-Methylbutyryl-CoA: 34.9
inactive: 32.7

BHS07_RS16380: mal

PKS_AT (2069..2355): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 189.2
inactive: 143.2
Methoxymalonyl-CoA: 68.3
Methylmalonyl-CoA: 55.4
Propionyl-CoA: 52.7
Ethylmalonyl-CoA: 30.6
Isobutyryl-CoA: 30.2
Benzoyl-CoA: 25.1
2-Methylbutyryl-CoA: 24.8
Acetyl-CoA: 24.2
trans-1,2-CPDA: 20.9
fatty_acid: 19.8
CHC-CoA: 19.0
3-Methylbutyryl-CoA: 11.3

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_CP017172.1 - Region 8 - T1PKS

Download region SVG

Location: 4,101,528 - 4,148,274 nt. (total: 46,747 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

BHS07_RS17515: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

BHS07_RS17520: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase methyltransferase

BHS07_RS17525: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BHS07_RS17535: methyltransferase

smcogs: SMCOG1089: methyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000393.5	0.42	NRPS, PKS	myxoprincomide-c506	Myxococcus xanthus DK 1622
BGC0000906.3	0.41	other (other)	heme D1	Heliophilum fasciatum
BGC0001909.3	0.41	PKS	strobilurin A	Strobilurus tenacellus
BGC0000905.5	0.41	other (other)	heme D1	Heliobacillus mobilis
BGC0000883.5	0.39	other (other)	arginomycin	Streptomyces arginensis
BGC0000662.5	0.39	terpene	grixazone A	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000188.5	0.39	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000885.3	0.39	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000889.5	0.39	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344
BGC0002000.4	0.39	PKS	setomimycin	Streptomyces aurantiacus JA 4570

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000393.5	0.42	NRPS, PKS	myxoprincomide-c506	Myxococcus xanthus DK 1622
BGC0000906.3	0.41	other (other)	heme D1	Heliophilum fasciatum
BGC0001909.3	0.41	PKS	strobilurin A	Strobilurus tenacellus
BGC0000905.5	0.41	other (other)	heme D1	Heliobacillus mobilis
BGC0000883.5	0.39	other (other)	arginomycin	Streptomyces arginensis
BGC0000662.5	0.39	terpene	grixazone A	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000188.5	0.39	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000885.3	0.39	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000889.5	0.39	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344
BGC0002000.4	0.39	PKS	setomimycin	Streptomyces aurantiacus JA 4570

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.31 out of 18.46

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.58 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.80 out of 23.79

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.67 out of 20.97

Confidence: medium

Additional weak hits (7):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.91 out of 26.85

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.21 out of 30.25

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.91 out of 32.93

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.24 out of 25.88

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.44 out of 18.57

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.29 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.57 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

BHS07_RS17525: mmal

PKS_AT (213..509): mmal

ATSignature: (unknown)

No matches above 50%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 39.5
Methoxymalonyl-CoA: 26.6
trans-1,2-CPDA: 22.9
Ethylmalonyl-CoA: 22.0
CHC-CoA: 20.3
Propionyl-CoA: 17.8
2-Methylbutyryl-CoA: 17.7
Acetyl-CoA: 11.8
fatty_acid: 10.7
Isobutyryl-CoA: 10.6
Benzoyl-CoA: 9.7
inactive: 0.0
3-Methylbutyryl-CoA: 0.0
Malonyl-CoA: -0.3

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence

NZ_CP017172.1 - Region 9 - RiPP-like

Download region SVG

Location: 4,241,197 - 4,252,060 nt. (total: 10,864 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001838.4	0.29	NRPS	WLIP	Pseudomonas putida
BGC0001588.3	0.24	saccharide	exopolysaccharides	Burkholderia pseudomallei 1026b
BGC0000714.5	0.22	saccharide	sisomicin	Micromonospora inyonensis
BGC0001603.3	0.22	saccharide	gentamicin	Micromonospora echinospora
BGC0002070.3	0.22	PKS	SEK90, pentangumycin	Saccharothrix espanaensis DSM 44229
BGC0001589.3	0.22	saccharide	exopolysaccharides	Burkholderia cenocepacia J2315
BGC0002025.4	0.21	NRPS, PKS	thaxteramide C	Jahnella sp. MSr9139
BGC0001093.3	0.21	NRPS, PKS	disorazol A	Sorangium cellulosum
BGC0000080.5	0.21	PKS	jerangolid A, jerangolid D	Sorangium cellulosum
BGC0000696.5	0.21	saccharide	gentamicin	Micromonospora echinospora

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001838.4	0.29	NRPS	WLIP	Pseudomonas putida
BGC0001588.3	0.24	saccharide	exopolysaccharides	Burkholderia pseudomallei 1026b
BGC0000714.5	0.22	saccharide	sisomicin	Micromonospora inyonensis
BGC0001603.3	0.22	saccharide	gentamicin	Micromonospora echinospora
BGC0002070.3	0.22	PKS	SEK90, pentangumycin	Saccharothrix espanaensis DSM 44229
BGC0001589.3	0.22	saccharide	exopolysaccharides	Burkholderia cenocepacia J2315
BGC0002025.4	0.21	NRPS, PKS	thaxteramide C	Jahnella sp. MSr9139
BGC0001093.3	0.21	NRPS, PKS	disorazol A	Sorangium cellulosum
BGC0000080.5	0.21	PKS	jerangolid A, jerangolid D	Sorangium cellulosum
BGC0000696.5	0.21	saccharide	gentamicin	Micromonospora echinospora

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator

NZ_CP017172.1 - Region 10 - NRP-metallophore,NRPS,T1PKS

Download region SVG

Location: 4,365,702 - 4,538,043 nt. (total: 172,342 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BHS07_RS19010: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase acyltransferase aminotransferase glycosyltransferase sulfotransferase

BHS07_RS18875: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BHS07_RS18885: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

BHS07_RS18915: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

BHS07_RS18925: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BHS07_RS18940: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

BHS07_RS18975: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: esterase hydrolase

BHS07_RS18910: thioesterase

smcogs: SMCOG1004: thioesterase

BHS07_RS18995: isochorismatase

smcogs: SMCOG1027: isochorismatase

BHS07_RS19075: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: dehydratase

BHS07_RS18990: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: isomerase

BHS07_RS19005: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

BHS07_RS18860: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BHS07_RS18865: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BHS07_RS18870: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS18920: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BHS07_RS18945: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS18950: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BHS07_RS19000: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	2.21	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	2.17	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	2.09	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	2.07	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	2.06	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	2.06	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	2.06	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	2.04	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	1.91	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	1.90	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002492.3	0.92	NRPS	myxochelin B, myxochelin A	Myxococcus xanthus DK 1622
BGC0001185.5	0.80	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0001758.4	0.77	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.77	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000343.5	0.77	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001641.4	0.77	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.76	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.75	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.72 out of 20.47

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 27.11 out of 29.38

Confidence: strong

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.31 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.65 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.33 out of 29.38

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.73 out of 28.19

Confidence: strong

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.38 out of 28.19

Confidence: medium

Additional weak hits (26):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.31 out of 30.22

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.18 out of 29.25

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 22.53 out of 33.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.90 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.64 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.95 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.60 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.03 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.24 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.39 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.04 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.91 out of 29.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.43 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.66 out of 32.74

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.81 out of 24.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.92 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.61 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.95 out of 28.14

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 21.08 out of 26.12

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.06 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.45 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.69 out of 24.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 17.16 out of 22.40

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.38 out of 23.43

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 14, location 4365701 - 4538043:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Lys) + (bLys - Val - Gln - X - Asp - X - X - Val - X - X - Ala) + (mal - D-Phe) + (fatty_acid - X - D-Ser - Ile - Val) + (pk) + (mal) + (mal) + (ohmal) + (ohmal) + (X - X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 15, location 4365701 - 4419405:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ohmal) + (ohmal) + (X - X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 16, location 4379464 - 4449491:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - X - D-Ser - Ile - Val) + (pk) + (mal) + (mal) + (ohmal) + (ohmal) + (X - X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 17, location 4412647 - 4538043:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (pk) + (fatty_acid - X - D-Ser - Ile - Val) + (mal - D-Phe) + (bLys - Val - Gln - X - Asp - X - X - Val - X - X - Ala) + (Lys)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS18860: X - X - X - X

AMP-binding (512..913): X

nrpys: bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LANAFDASTLEGWLLAGGDVNGYGPTENTTFSAT
Nearest Stachelhaus code(s):: bOH-Val DALWLGGTFK (85% 8Å match); Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1584..1981): X

nrpys: Gln, Glu, Ile

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LALAFDASVQQVDCLAGGEFNVYGPTECTVDSTL
Nearest Stachelhaus code(s):: Gln or Glu DAQDLGVVDK (85% 8Å match); Ile DAQDLGVVDK (85% 8Å match); Gln DAQDLGVVDK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2643..3044): X

nrpys: bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LENAFDASTLEGWLLAGGDVNGYGPTENTTFSAT
Nearest Stachelhaus code(s):: bOH-Val DALWLGGTFK (85% 8Å match); Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3712..4109): X

nrpys: Gln, Glu, Ile

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LALAFDASVQQVDCLAGGEFNVYGPTECTVDSTL
Nearest Stachelhaus code(s):: Gln or Glu DAQDLGVVDK (85% 8Å match); Ile DAQDLGVVDK (85% 8Å match); Gln DAQDLGVVDK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS18865: mal

PKS_AT (535..820): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 104.4
inactive: 74.0
Methoxymalonyl-CoA: 61.7
Methylmalonyl-CoA: 48.3
2-Methylbutyryl-CoA: 33.4
Propionyl-CoA: 33.2
Ethylmalonyl-CoA: 32.6
Benzoyl-CoA: 19.1
Isobutyryl-CoA: 14.2
Acetyl-CoA: 12.7
CHC-CoA: 12.0
fatty_acid: 6.6
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: -1.2

BHS07_RS18870: mal

PKS_AT (538..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; 2-Rhyd-Malonyl-CoA: 58.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 130.2
inactive: 87.4
Methylmalonyl-CoA: 74.3
Methoxymalonyl-CoA: 67.2
Ethylmalonyl-CoA: 52.4
2-Methylbutyryl-CoA: 51.5
Propionyl-CoA: 45.4
Isobutyryl-CoA: 32.6
Benzoyl-CoA: 32.4
CHC-CoA: 27.1
Acetyl-CoA: 19.8
3-Methylbutyryl-CoA: 14.7
trans-1,2-CPDA: 10.2
fatty_acid: 8.9

BHS07_RS18875: mal

PKS_AT (538..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 179.8
inactive: 131.8
Methoxymalonyl-CoA: 66.0
Methylmalonyl-CoA: 57.9
Propionyl-CoA: 45.0
Isobutyryl-CoA: 28.7
Benzoyl-CoA: 23.2
Ethylmalonyl-CoA: 22.4
CHC-CoA: 21.9
2-Methylbutyryl-CoA: 20.6
Acetyl-CoA: 20.4
trans-1,2-CPDA: 14.1
fatty_acid: 10.9
3-Methylbutyryl-CoA: 0.0

BHS07_RS18920: mal

PKS_AT (532..812): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 186.0
inactive: 101.8
Methoxymalonyl-CoA: 80.7
Methylmalonyl-CoA: 67.8
Propionyl-CoA: 47.9
Isobutyryl-CoA: 46.7
Ethylmalonyl-CoA: 44.3
2-Methylbutyryl-CoA: 37.0
CHC-CoA: 29.9
Benzoyl-CoA: 28.1
trans-1,2-CPDA: 26.5
fatty_acid: 24.7
Acetyl-CoA: 21.6
3-Methylbutyryl-CoA: 11.4

BHS07_RS18925: pk

PKS_AT (537..831): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 62.5%; Malonyl-CoA: 54.2%; Ethylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 136.1
inactive: 77.2
Methoxymalonyl-CoA: 72.4
Methylmalonyl-CoA: 66.8
Propionyl-CoA: 43.5
Ethylmalonyl-CoA: 42.7
2-Methylbutyryl-CoA: 30.5
Isobutyryl-CoA: 27.8
Benzoyl-CoA: 27.3
Acetyl-CoA: 19.4
CHC-CoA: 18.2
3-Methylbutyryl-CoA: 15.0
trans-1,2-CPDA: 11.2
fatty_acid: 10.0

BHS07_RS18940: fatty_acid - X - Ser - Ile - Val

Search NORINE for peptide: strict or relaxed

CAL_domain (38..487): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 27.3
NH2: 15.7
AHBA: 15.5
shikimic_acid: 0.0
Acetyl-CoA: -1.2

A-OX (1167..1931): X

No predictions made

AMP-binding (2568..2968): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFCSGEHNLYGPTEAAVDVTA
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4045..4445): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: LETSFDAATFEGFLHFGGELHVYGPTENTTFSTW
Nearest Stachelhaus code(s):: Ile DAFFLGVTFK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (5106..5502): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGSAFDAATFEGWLLAGGDVNGYGPTEGTTFTCC
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS18945: mal - X - Phe

Search NORINE for peptide: strict or relaxed

PKS_AT (532..829): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Propionyl-CoA: 54.2%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 120.6
Methoxymalonyl-CoA: 63.6
inactive: 60.2
Methylmalonyl-CoA: 57.3
Ethylmalonyl-CoA: 41.7
Isobutyryl-CoA: 37.8
CHC-CoA: 34.7
Propionyl-CoA: 29.8
Benzoyl-CoA: 23.3
fatty_acid: 18.6
2-Methylbutyryl-CoA: 17.1
trans-1,2-CPDA: 10.8
Acetyl-CoA: 9.8
3-Methylbutyryl-CoA: 8.2

A-OX (1532..2151): X

No predictions made

AMP-binding (2757..3148): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: TTIAFDASVLESTLISAGELNGYGPTEASVCATL
Nearest Stachelhaus code(s):: Phe DAWTIAGVCK (59% 8Å match)
Stachelhaus code match:: 90% (moderate)

BHS07_RS18950: bLys - Val - Gln - X - Asp - X - X - Val - X - X - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (494..899): bLys

nrpys: bLys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAEAWDTSAEEPNLVIGGELNTYGLTEVTAVATA
Nearest Stachelhaus code(s):: bLys DTENVGTAVK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1561..1957): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LETAFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (85% 8Å match); bOH-Val DALWLGGTFK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2623..3017): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HWFAFDASVPEAQLVSGGEINAYGPTETTVDATA
Nearest Stachelhaus code(s):: Arg DAESVGAVDK (74% 8Å match); Gln DAWQVGVVDK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (3679..4075): X

nrpys: Val, bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGTAFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (82% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4736..5140): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YGASADLGHTAPKVVLGGDFNHYGPSETTVGVLT
Nearest Stachelhaus code(s):: Asp DLTKVGHVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5802..6198): X

nrpys: Val, bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGTAFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (82% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6861..7258): X

nrpys: Val, bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGTAFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (82% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7923..8319): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDTAFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (82% 8Å match); bOH-Val DALWLGGTFK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (8981..9378): X

nrpys: Val, bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGTAFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (82% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (10041..10437): X

nrpys: Val, bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGTAFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (82% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (11100..11493): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: FWTTFDLSVFEANTNLAGELNLYGPTEDTTYSTW
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS18990: Lys

Search NORINE for peptide: strict or relaxed

AMP-binding (455..860): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGAAFDASVEEVDLIIGGELNTYGPTEATVVATV
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS19000: X

AMP-binding (35..440): X

nrpys: diOH-Bz, Sal

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SGSLFPLSSPGGAVQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz or Sal PLPAQGVVNK (97% 8Å match); diOH-Bz PLPAQGVVNK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
FuR variant 1	Ferric Uptake Regulator
VqsM	Regulator of quorum-sensing signalling systems
ZuR variant 1	Zinc-responsive repressor

NZ_CP017172.1 - Region 11 - terpene-precursor

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Location: 4,555,122 - 4,576,120 nt. (total: 20,999 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: glucanase glucosidase

BHS07_RS19245: Pullulanase, type I

smcogs: SMCOG1297: Pullulanase, type I

BHS07_RS19255: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

BHS07_RS19260: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Ligases: synthetase

BHS07_RS19225: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.45	terpene	astallatene	Arabidopsis thaliana
BGC0002285.2	0.45	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002400.2	0.42	terpene	boleracene	Brassica oleracea var. oleracea
BGC0001285.5	0.40	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0001075.5	0.40	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000676.5	0.39	terpene	aphidicolin, aphidicolan-16β-ol, 3-deoxyaphidicolin, 17-deoxyaphidicolin	Phoma betae
BGC0000905.5	0.37	other (other)	heme D1	Heliobacillus mobilis
BGC0002398.2	0.37	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000906.3	0.37	other (other)	heme D1	Heliophilum fasciatum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.45	terpene	astallatene	Arabidopsis thaliana
BGC0002285.2	0.45	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002400.2	0.42	terpene	boleracene	Brassica oleracea var. oleracea
BGC0001285.5	0.40	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0001075.5	0.40	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000676.5	0.39	terpene	aphidicolin, aphidicolan-16β-ol, 3-deoxyaphidicolin, 17-deoxyaphidicolin	Phoma betae
BGC0000905.5	0.37	other (other)	heme D1	Heliobacillus mobilis
BGC0002398.2	0.37	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000906.3	0.37	other (other)	heme D1	Heliophilum fasciatum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

BHS07_RS19225: PT_FPPS_like

Domain 1 (42 - 305): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (9):

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.02 out of 20.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.56 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.25 out of 26.06

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.15 out of 25.75

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.37 out of 29.38

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.32 out of 30.98

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.75 out of 26.85

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.43 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.95 out of 32.93

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_CP017172.1 - Region 12 - RRE-containing

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Location: 4,611,062 - 4,631,358 nt. (total: 20,297 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: kinase

BHS07_RS19445: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001645.3	0.42	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0002674.2	0.40	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0001364.3	0.39	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0001655.3	0.38	ribosomal	LP2006	Nocardiopsis alba DSM 43377
BGC0000496.5	0.37	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001660.3	0.37	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0000563.4	0.36	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000626.3	0.36	ribosomal	thuricin	Bacillus thuringiensis
BGC0001226.4	0.36	ribosomal	streptocollin	Streptomyces collinus Tu 365

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001645.3	0.42	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0002674.2	0.40	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0001364.3	0.39	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0001655.3	0.38	ribosomal	LP2006	Nocardiopsis alba DSM 43377
BGC0000496.5	0.37	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001660.3	0.37	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0000563.4	0.36	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000626.3	0.36	ribosomal	thuricin	Bacillus thuringiensis
BGC0001226.4	0.36	ribosomal	streptocollin	Streptomyces collinus Tu 365

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.34 out of 27.55

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.13 out of 23.79

Confidence: medium

Additional weak hits (3):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.27 out of 20.01

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.17 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.15 out of 24.78

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 12 - 92; Score: 42.4; RRE type: PqqD_RRE; Location: 4 - 92; Score: 30.6

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
GnfM	Regulator of nitrogen-fixation genes
IolR	Regulator of the inositol catabolism

NZ_CP017172.1 - Region 13 - NRPS,T1PKS

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Location: 4,660,184 - 4,746,569 nt. (total: 86,386 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BHS07_RS19810: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acetyltransferase methyltransferase

BHS07_RS19795: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

BHS07_RS40160: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: synthase

BHS07_RS19715: carbamoyl phosphate synthase small subunit

smcogs: SMCOG1249: carbamoyl phosphate synthase small subunit

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000393.5	1.38	NRPS, PKS	myxoprincomide-c506	Myxococcus xanthus DK 1622
BGC0001758.4	0.94	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.92	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.89	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.88	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.87	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.85	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.85	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000307.4	0.80	NRPS	AbT1	Aureobasidium pullulans
BGC0001844.4	0.50	NRPS	holrhizin A, holrhizin B, holrhizin C, holrhizin D	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000393.5	0.88	NRPS, PKS	myxoprincomide-c506	Myxococcus xanthus DK 1622
BGC0001758.4	0.77	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.75	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.74	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.74	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.74	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000307.4	0.72	NRPS	AbT1	Aureobasidium pullulans
BGC0001844.4	0.58	NRPS	holrhizin A, holrhizin B, holrhizin C, holrhizin D	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (10):

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.72 out of 20.97

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.62 out of 26.12

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.61 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.74 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.79 out of 27.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.35 out of 24.78

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.58 out of 30.11

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.32 out of 28.14

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.78 out of 26.12

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 20, location 4660183 - 4746569:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - NMe-Ser - X - Leu - X - Ser - Val - mal - Ser - Tyr - bLys - Ala - Ser)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS40160: fatty_acid - Ser - X - Leu - X - Ser - Val - mal - X - Ser - Tyr - bLys - Ala - Ser

Search NORINE for peptide: strict or relaxed

CAL_domain (41..490): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 18.7
AHBA: 13.8
NH2: 11.4
shikimic_acid: 5.1
Acetyl-CoA: -2.6

AMP-binding (1164..1565): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAVDVTY
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2640..3037): X

nrpys: bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGSAFDASTLEGWLLAGGDVNGYGPTEGTTFTAC
Nearest Stachelhaus code(s):: bOH-Val DALWLGGTFK (97% 8Å match); Val DALWLGGTFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3697..4094): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGSSFDVSSVEGILLTGGETNAYGPTECSTMATR
Nearest Stachelhaus code(s):: Leu DVVLMGATMK (85% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (4755..5152): X

nrpys: bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGSAFDASTLEGWLLAGGDVNGYGPTEGTTFTAC
Nearest Stachelhaus code(s):: bOH-Val DALWLGGTFK (97% 8Å match); Val DALWLGGTFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5812..6213): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAVDVTY
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6879..7278): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSEAFDGSVMEIHFHVVGDTNGYGPTENSIISAS
Nearest Stachelhaus code(s):: Val DGMHNVGIIK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

PKS_AT (8014..8310): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 140.4
Methoxymalonyl-CoA: 76.3
Methylmalonyl-CoA: 61.9
inactive: 57.5
Propionyl-CoA: 38.7
Isobutyryl-CoA: 34.2
Ethylmalonyl-CoA: 31.3
CHC-CoA: 28.0
Benzoyl-CoA: 27.1
Acetyl-CoA: 24.9
2-Methylbutyryl-CoA: 23.6
trans-1,2-CPDA: 17.0
fatty_acid: 14.2
3-Methylbutyryl-CoA: 11.1

A-OX (9122..9846): X

No predictions made

AMP-binding (10355..10756): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAVDVTY
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (11416..11815): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MGTAFDGALEPHDCVCAGEYNAYGPTESTVCATT
Nearest Stachelhaus code(s):: Tyr DGEDVAAVCK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (12479..12886): bLys

nrpys: bLys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAAAWDTSAEEPNLVIGGELNTYGLTEVTAVATS
Nearest Stachelhaus code(s):: bLys DTENVGTAVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (13550..13950): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGMAFDGTVERHDCFCGGEFNVYGPTESTVCATS
Nearest Stachelhaus code(s):: Ala DGEDFGVVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (14609..15009): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LASAFDVSMDQHDCLCAGEFNAYGPTESTVYATF
Nearest Stachelhaus code(s):: Ser DVGYLAAVYK (50% 8Å match)
Stachelhaus code match:: 80% (moderate)

TFBS definitions

Regulator	Description

NZ_CP017172.1 - Region 14 - NRPS,T1PKS

Download region SVG

Location: 4,957,379 - 5,027,579 nt. (total: 70,201 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

BHS07_RS20785: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acetyltransferase

BHS07_RS20825: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Lyases: dehydratase

BHS07_RS20705: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

BHS07_RS20770: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BHS07_RS20775: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS20780: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.93	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.92	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002075.3	0.90	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001128.4	0.90	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.89	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.88	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.88	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.87	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.87	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001133.4	0.86	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.76	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002075.3	0.75	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.75	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.75	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.74	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.74	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001133.4	0.74	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.78 out of 29.72

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (11):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.55 out of 30.22

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.00 out of 36.54

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.16 out of 30.22

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.05 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.17 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.89 out of 29.65

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.58 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.07 out of 33.14

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.58 out of 35.63

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.64 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.66 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 21, location 4957378 - 5027579:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NH2 - X) + (mal - D-X) + (Asp - X - Ser - X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS20770: Asp - X - Ser - X

Search NORINE for peptide: strict or relaxed

AMP-binding (467..871): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YAASADLGNTVPKLVLGGEYNHYGPTEATVGVLA
Nearest Stachelhaus code(s):: Asp DLTKVGHVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1555..1950): X

nrpys: bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGSAFDASTLEGWLLAGGDINGYGPTEGTTFTTV
Nearest Stachelhaus code(s):: bOH-Val DALWLGGTFK (88% 8Å match); Val DALWLGGTFK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2608..3012): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YAASADLGNTVAKVVVGGEHNHYGPTETTVGVLA
Nearest Stachelhaus code(s):: Ser DLTKVGHVGK (79% 8Å match); Asp DLTKVGHVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3669..4063): X

nrpys: bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGSAFDASTLEGWLLAGGDINGYGPTEGTTFTTV
Nearest Stachelhaus code(s):: bOH-Val DALWLGGTFK (88% 8Å match); Val DALWLGGTFK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS20775: mal - X - X

PKS_AT (535..829): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 163.0
inactive: 81.1
Methoxymalonyl-CoA: 71.2
Methylmalonyl-CoA: 59.6
CHC-CoA: 37.9
Propionyl-CoA: 35.7
Ethylmalonyl-CoA: 34.4
Isobutyryl-CoA: 34.2
fatty_acid: 21.3
Benzoyl-CoA: 19.0
2-Methylbutyryl-CoA: 18.6
Acetyl-CoA: 11.1
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

A-OX (1500..2121): X

No predictions made

AMP-binding (2729..3122): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AARAFDASLAGWVSSVGGEFNEYGPTEATIWSTV
Nearest Stachelhaus code(s):: Trp DAAIIGEIWK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

BHS07_RS20780: NH2 - X

CAL_domain (26..474): NH2

minowa: NH2

Prediction, score:

NH2: 175.4
AHBA: 23.8
fatty_acid: 1.7
shikimic_acid: 0.0
Acetyl-CoA: -3.1

AMP-binding (1151..1545): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSMAFDMSAWDLTLMVAAEYNGYGPTEVTVGATW
Nearest Stachelhaus code(s):: Kyn DAWTTTGVGK (65% 8Å match); Phe DAWTIAGVCK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
NtrC	Nitrogen regulatory protein C

NZ_CP017172.1 - Region 15 - NRPS,T1PKS

Download region SVG

Location: 5,082,322 - 5,148,224 nt. (total: 65,903 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: back translocase

BHS07_RS21200: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

Ligases: ligase synthase

BHS07_RS21175: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BHS07_RS21180: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BHS07_RS21185: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001427.4	1.07	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001426.4	1.06	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001425.4	1.05	NRPS, PKS	myxochromide A	Myxococcus xanthus DK 1622
BGC0001423.4	1.03	NRPS, PKS	myxochromide C	Myxococcus hansupus
BGC0001023.5	0.99	NRPS, PKS	myxochromide S1	Stigmatella aurantiaca
BGC0001424.4	0.93	NRPS, PKS	myxochromide C	Myxococcus virescens
BGC0001421.4	0.90	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0001428.4	0.90	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0002075.3	0.84	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.84	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001427.4	0.86	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001426.4	0.86	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001425.4	0.85	NRPS, PKS	myxochromide A	Myxococcus xanthus DK 1622
BGC0001423.4	0.84	NRPS, PKS	myxochromide C	Myxococcus hansupus
BGC0001424.4	0.81	NRPS, PKS	myxochromide C	Myxococcus virescens
BGC0001421.4	0.81	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0001428.4	0.80	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001023.5	0.78	NRPS, PKS	myxochromide S1	Stigmatella aurantiaca
BGC0001422.4	0.75	NRPS, PKS	myxochromide A	Myxococcus fulvus HW-1
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.34 out of 23.79

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.10 out of 28.19

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (7):

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 22.07 out of 32.93

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.37 out of 27.78

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.82 out of 39.62

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.86 out of 24.78

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.33 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.49 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.00 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 22, location 5082321 - 5148224:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (NMe-Thr - D-Ala) + (Leu - Pro - Gln)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS21175: pk

PKS_AT (544..840): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; CE-Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 112.8
Malonyl-CoA: 103.8
Isobutyryl-CoA: 98.1
Methoxymalonyl-CoA: 93.4
Ethylmalonyl-CoA: 81.0
2-Methylbutyryl-CoA: 51.5
Propionyl-CoA: 51.3
Benzoyl-CoA: 46.0
trans-1,2-CPDA: 40.5
CHC-CoA: 40.1
Acetyl-CoA: 31.2
3-Methylbutyryl-CoA: 24.3
inactive: 22.9
fatty_acid: 19.9

BHS07_RS21180: Thr - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (508..912): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LTSHFDFSVWEGNQIFGGEINMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2007..2397): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: IASAFDVSVWDMVLITAGEVNAYGPTETTVIATV
Nearest Stachelhaus code(s):: Ala DVWVIAAVIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS21185: Leu - Pro - Gln

Search NORINE for peptide: strict or relaxed

AMP-binding (479..868): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDAAVWEPFLLTGGDVNNYGPTETTVVATS
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1517..1912): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVSFQESFLITAGDHNHYGPTESHVVTAH
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2564..2955): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HWMTFDASVWEAQLCVGGEWNFYGPTEATIEAVV
Nearest Stachelhaus code(s):: Gln DAWQCGFIEK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
IolR	Regulator of the inositol catabolism
ZuR variant 1	Zinc-responsive repressor

NZ_CP017172.1 - Region 16 - RiPP-like

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Location: 5,209,699 - 5,221,351 nt. (total: 11,653 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.39	PKS	curacin A	Moorea producens 19L
BGC0002571.2	0.35	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0000527.5	0.34	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000547.5	0.34	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0002007.2	0.33	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0000556.5	0.33	ribosomal	streptin	Streptococcus pyogenes
BGC0000515.4	0.33	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000506.4	0.33	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0002346.3	0.32	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000557.5	0.32	ribosomal	streptococcin A-FF22	Streptococcus pyogenes

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.39	PKS	curacin A	Moorea producens 19L
BGC0002571.2	0.35	NRPS	pyoverdine DC3000	Pseudomonas syringae pv. tomato str. DC3000
BGC0000527.5	0.34	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000547.5	0.34	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0002007.2	0.33	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0000556.5	0.33	ribosomal	streptin	Streptococcus pyogenes
BGC0000515.4	0.33	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000506.4	0.33	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0002346.3	0.32	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000557.5	0.32	ribosomal	streptococcin A-FF22	Streptococcus pyogenes

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.52 out of 23.79

Confidence: medium

Additional weak hits (3):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.00 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.01 out of 26.06

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.38 out of 32.93

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
IolR	Regulator of the inositol catabolism

NZ_CP017172.1 - Region 17 - NRPS,T1PKS

Download region SVG

Location: 5,354,025 - 5,430,931 nt. (total: 76,907 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

BHS07_RS22370: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

BHS07_RS22380: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acylase

BHS07_RS22320: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BHS07_RS22345: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase hydrolase

BHS07_RS22260: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

BHS07_RS22310: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: decarboxylase

BHS07_RS22265: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: ligase synthase

BHS07_RS22340: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BHS07_RS22350: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS22355: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS22360: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BHS07_RS22365: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS22385: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.96	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.96	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.96	PKS	asperlactone	Aspergillus ochraceus
BGC0000342.4	0.73	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001276.3	0.69	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.77	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001276.3	0.68	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.81 out of 28.19

Confidence: medium

Additional weak hits (9):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.20 out of 27.78

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.60 out of 26.12

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.82 out of 30.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.39 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.94 out of 29.38

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.07 out of 21.55

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.78 out of 20.01

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.96 out of 32.74

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 24, location 5354024 - 5430931:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr - ccmal) + (ccmal) + (ccmal) + (Asn) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS22320: mal

PKS_AT (563..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.3
inactive: 132.8
Methoxymalonyl-CoA: 55.4
Methylmalonyl-CoA: 54.3
Propionyl-CoA: 41.8
Benzoyl-CoA: 31.5
Isobutyryl-CoA: 31.1
Acetyl-CoA: 23.4
Ethylmalonyl-CoA: 22.5
trans-1,2-CPDA: 14.8
2-Methylbutyryl-CoA: 14.5
CHC-CoA: 10.4
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

BHS07_RS22340: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (536..940): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWSGFDGTLTSAKMVLGGEINEYGPTEAAVACCF
Nearest Stachelhaus code(s):: Asn DATKVGEVGK (74% 8Å match); OH-Asn or Asn DATKVGEVGK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

BHS07_RS22345: mal

PKS_AT (558..851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Propionyl-CoA: 62.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 180.8
Methoxymalonyl-CoA: 95.9
inactive: 95.7
Methylmalonyl-CoA: 87.5
Propionyl-CoA: 60.3
Ethylmalonyl-CoA: 60.3
Isobutyryl-CoA: 47.7
2-Methylbutyryl-CoA: 47.5
Acetyl-CoA: 29.9
trans-1,2-CPDA: 26.6
CHC-CoA: 25.8
Benzoyl-CoA: 25.1
fatty_acid: 24.5
3-Methylbutyryl-CoA: 6.4

BHS07_RS22350: mal

PKS_AT (567..857): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 184.2
inactive: 122.2
Methoxymalonyl-CoA: 73.3
Methylmalonyl-CoA: 52.9
Propionyl-CoA: 32.7
Isobutyryl-CoA: 30.7
Acetyl-CoA: 20.3
fatty_acid: 19.7
Benzoyl-CoA: 18.1
3-Methylbutyryl-CoA: 17.6
2-Methylbutyryl-CoA: 17.6
Ethylmalonyl-CoA: 14.7
CHC-CoA: 13.9
trans-1,2-CPDA: 11.5

BHS07_RS22355: Thr - mal

Search NORINE for peptide: strict or relaxed

AMP-binding (542..941): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: GWTSHDFSVYDGSTMLGGDMSVGGPTETSLWNIS
Nearest Stachelhaus code(s):: Thr DFYSMGVLWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1681..1975): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 62.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 133.5
inactive: 89.1
Methoxymalonyl-CoA: 57.0
Methylmalonyl-CoA: 54.5
Benzoyl-CoA: 26.0
Propionyl-CoA: 24.0
Isobutyryl-CoA: 20.9
Ethylmalonyl-CoA: 18.8
trans-1,2-CPDA: 17.9
2-Methylbutyryl-CoA: 13.4
Acetyl-CoA: 10.2
CHC-CoA: 9.6
3-Methylbutyryl-CoA: 8.9
fatty_acid: 0.0

BHS07_RS22385: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (8..409): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FWCAFDLSVLDAYSLFAGEFNLYGPTETNVCTFH
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems
ZuR variant 1	Zinc-responsive repressor

NZ_CP017172.1 - Region 18 - NRPS,T1PKS

Download region SVG

Location: 5,506,060 - 5,576,211 nt. (total: 70,152 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BHS07_RS22990: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acylase

BHS07_RS22885: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

BHS07_RS22830: putative esterase

smcogs: SMCOG1104: putative esterase

BHS07_RS22840: thioesterase superfamily protein

smcogs: SMCOG1144: thioesterase superfamily protein

Ligases: ligase

BHS07_RS22890: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BHS07_RS22895: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BHS07_RS22900: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BHS07_RS22910: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.94	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.90	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.89	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.88	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.85	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.83	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.80	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002071.2	0.80	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001825.4	0.79	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001166.5	0.79	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.78	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.78	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.78	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.77	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.76	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.76	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002075.3	0.75	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.75	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002071.2	0.73	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.86 out of 18.57

Confidence: medium

Additional weak hits (3):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.59 out of 26.06

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.62 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 25, location 5506059 - 5576211:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - X) + (Gly) + (Thr - X - D-Phe - Orn) + (Orn)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS22885: mal - X

PKS_AT (649..944): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 91.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 179.3
inactive: 136.3
Methoxymalonyl-CoA: 85.6
Methylmalonyl-CoA: 67.1
2-Methylbutyryl-CoA: 56.5
Isobutyryl-CoA: 56.3
Propionyl-CoA: 54.8
Ethylmalonyl-CoA: 46.0
CHC-CoA: 32.1
trans-1,2-CPDA: 27.0
Acetyl-CoA: 21.0
Benzoyl-CoA: 20.2
3-Methylbutyryl-CoA: 15.0
fatty_acid: 13.1

AMP-binding (2133..2537): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HDMAFDASLEQPNFIIGGDLNAYGPTETLITATT
Nearest Stachelhaus code(s):: Arg DAEDIGAITK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

BHS07_RS22890: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (538..928): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDISVLELQALCGGEWNMYGPTETTIWSTT
Nearest Stachelhaus code(s):: Gly DILQLGMIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS22895: Thr - X - Phe - Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (470..879): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGNQVFGGEINMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1550..1952): X

nrpys: Gln, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWATFDATITSTKLVVGGEINEYGPTETVVGCSI
Nearest Stachelhaus code(s):: Gln or Asn DATKVGEVGK (88% 8Å match); Asn DATKVGEVGK (85% 8Å match); OH-Asn or Asn DATKVGEVGK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2615..3005): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TAAAFDASVCESTLVSAGEINAYGPTEATICASV
Nearest Stachelhaus code(s):: Phe DAWTVAAICK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (4127..4535): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: AGWAFDVFAGDREFVAGSDINSYGVSEATIDSTW
Nearest Stachelhaus code(s):: Orn DVGEVGSIDK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS22900: Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (515..919): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: AAWAFDVFSGDREFIVGSDVSSYGLSEATIDSTY
Nearest Stachelhaus code(s):: Orn DVGEIGSIDK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS22910: NH2

CAL_domain (164..486): NH2

minowa: NH2

Prediction, score:

NH2: 173.4
Acetyl-CoA: 36.8
fatty_acid: 21.1
shikimic_acid: 0.0
AHBA: 0.0

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
ToxT	Regulator of MSHA biosynthesis

NZ_CP017172.1 - Region 19 - NRPS,T1PKS

Download region SVG

Location: 5,670,946 - 5,763,629 nt. (total: 92,684 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

BHS07_RS23405: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

BHS07_RS23410: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase

BHS07_RS23450: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: ligase synthase

BHS07_RS23430: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BHS07_RS23435: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BHS07_RS23440: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS23445: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BHS07_RS23455: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BHS07_RS23465: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	1.19	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	1.18	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	1.18	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	1.17	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001135.5	1.00	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	1.00	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001164.3	0.98	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.98	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001758.4	0.95	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000307.4	0.94	NRPS	AbT1	Aureobasidium pullulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001187.4	0.69	NRPS, PKS	xenolozoyenone	Glarea lozoyensis
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001737.3	0.68	NRPS, PKS	phenalamide	Corallococcus coralloides
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001022.5	0.68	NRPS, PKS	myxalamid, myxalamid A, myxalamid C, myxalamid D	Stigmatella aurantiaca
BGC0001164.3	0.67	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (19):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.74 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.56 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.14 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.39 out of 31.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.21 out of 21.55

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.90 out of 24.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.80 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.91 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.52 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.50 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.18 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.48 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.35 out of 28.14

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.81 out of 25.88

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.71 out of 25.88

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.93 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.97 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.97 out of 33.14

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.10 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 26, location 5670945 - 5763629:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - X) + (Me-ccmal) + (Me-ccmal) + (pk - pk - ccmal) + (Me-redmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 27, location 5670945 - 5755662:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - X) + (Me-ccmal) + (Me-ccmal) + (pk - pk - ccmal) + (Me-redmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 28, location 5717164 - 5763629:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - X) + (Me-ccmal) + (Me-ccmal) + (pk - pk - ccmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BHS07_RS23430: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (542..932): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: FWATFDLSVFETNTNLAGEFNLYGPSETTTYSTF
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BHS07_RS23435: mmal

PKS_AT (567..865): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.5
Ethylmalonyl-CoA: 156.5
Methoxymalonyl-CoA: 139.9
Malonyl-CoA: 111.3
Isobutyryl-CoA: 104.4
Benzoyl-CoA: 94.2
2-Methylbutyryl-CoA: 93.4
Acetyl-CoA: 89.0
trans-1,2-CPDA: 82.9
CHC-CoA: 82.9
Propionyl-CoA: 81.7
3-Methylbutyryl-CoA: 57.0
fatty_acid: 45.7
inactive: 23.6

BHS07_RS23440: pk - pk - mal

PKS_AT (560..858): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; Methylmalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 141.2
Ethylmalonyl-CoA: 129.9
Malonyl-CoA: 127.6
Methoxymalonyl-CoA: 117.3
Isobutyryl-CoA: 85.7
2-Methylbutyryl-CoA: 84.3
Acetyl-CoA: 76.7
trans-1,2-CPDA: 66.9
Benzoyl-CoA: 58.4
Propionyl-CoA: 55.8
CHC-CoA: 44.7
inactive: 38.7
3-Methylbutyryl-CoA: 38.5
fatty_acid: 22.4

PKS_AT (2370..2666): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 75.0%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 130.4
Malonyl-CoA: 126.5
Methoxymalonyl-CoA: 120.4
Isobutyryl-CoA: 104.9
Ethylmalonyl-CoA: 94.5
Benzoyl-CoA: 68.2
Propionyl-CoA: 63.6
2-Methylbutyryl-CoA: 58.1
Acetyl-CoA: 57.0
inactive: 54.6
CHC-CoA: 53.7
fatty_acid: 50.8
3-Methylbutyryl-CoA: 48.9
trans-1,2-CPDA: 36.6

PKS_AT (3893..4186): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.3
inactive: 165.5
Methoxymalonyl-CoA: 93.0
Methylmalonyl-CoA: 90.0
Propionyl-CoA: 73.9
2-Methylbutyryl-CoA: 58.3
Isobutyryl-CoA: 57.3
Ethylmalonyl-CoA: 56.3
CHC-CoA: 46.1
trans-1,2-CPDA: 39.8
Benzoyl-CoA: 33.4
Acetyl-CoA: 28.0
3-Methylbutyryl-CoA: 26.5
fatty_acid: 21.0

BHS07_RS23445: mmal

PKS_AT (568..865): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 173.6
Ethylmalonyl-CoA: 155.4
Methoxymalonyl-CoA: 126.4
Benzoyl-CoA: 99.2
Malonyl-CoA: 93.3
trans-1,2-CPDA: 91.7
Isobutyryl-CoA: 86.1
Acetyl-CoA: 78.2
2-Methylbutyryl-CoA: 75.0
Propionyl-CoA: 65.4
CHC-CoA: 52.6
inactive: 39.9
3-Methylbutyryl-CoA: 36.1
fatty_acid: 22.0

BHS07_RS23450: mmal

PKS_AT (563..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 172.7
Ethylmalonyl-CoA: 147.4
Methoxymalonyl-CoA: 135.6
Isobutyryl-CoA: 98.6
Malonyl-CoA: 90.8
Benzoyl-CoA: 89.7
2-Methylbutyryl-CoA: 77.2
trans-1,2-CPDA: 67.8
Propionyl-CoA: 57.3
Acetyl-CoA: 56.0
CHC-CoA: 50.4
3-Methylbutyryl-CoA: 47.3
inactive: 35.4
fatty_acid: 17.8

BHS07_RS23455: pk - mmal

PKS_AT (606..903): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 66.7%; Malonyl-CoA: 54.2%; Methylmalonyl-CoA: 54.2%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 146.1
Benzoyl-CoA: 122.0
Methylmalonyl-CoA: 99.7
Methoxymalonyl-CoA: 81.0
Ethylmalonyl-CoA: 80.7
Malonyl-CoA: 74.1
2-Methylbutyryl-CoA: 57.6
Acetyl-CoA: 48.5
Propionyl-CoA: 46.8
CHC-CoA: 36.4
trans-1,2-CPDA: 29.8
3-Methylbutyryl-CoA: 24.9
inactive: 22.8
fatty_acid: 16.3

PKS_AT (1032..1328): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 185.8
Ethylmalonyl-CoA: 168.9
Methoxymalonyl-CoA: 149.8
Benzoyl-CoA: 112.3
Isobutyryl-CoA: 112.2
Malonyl-CoA: 103.1
2-Methylbutyryl-CoA: 101.5
trans-1,2-CPDA: 89.0
Propionyl-CoA: 87.0
CHC-CoA: 66.6
Acetyl-CoA: 55.0
3-Methylbutyryl-CoA: 40.0
fatty_acid: 39.9
inactive: 26.7

BHS07_RS23465: fatty_acid - X

CAL_domain (47..499): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 26.9
AHBA: 16.7
NH2: 15.9
Acetyl-CoA: 0.3
shikimic_acid: 0.0

A-OX (1199..1969): X

No predictions made

TFBS definitions

Regulator	Description

NZ_CP017172.1 - Region 20 - terpene-precursor

Download region SVG

Location: 5,866,322 - 5,887,215 nt. (total: 20,894 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthase synthetase

BHS07_RS23955: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

BHS07_RS23960: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000665.5	0.37	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000668.5	0.36	other (other), terpene	12-epi-hapalindole C isonitrile, 12-epi-hapalindole E, 12-epi-fischerindole U isonitrile, fischerindole L, 12-epi-fischerindole I isonitrile, welwitindolinone A isonitrile, welwitindolinone B isothiocyanate, welwitindolinone C isothiocyanate, N-methylwelwitindolinone C isothiocyanate, N-methylwelwitinsolinone C isonitrile, 3-epi-welwitindolinone B isothiocyanate, 3-(Z-2'-isocyanoethenyl)-indole	Hapalosiphon welwitschii UTEX B 1830
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000643.3	0.36	terpene	carotenoid	Brevundimonas vesicularis
BGC0001605.3	0.36	terpene	gibberellin	Sinorhizobium fredii NGR234

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000665.5	0.37	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000668.5	0.36	other (other), terpene	12-epi-hapalindole C isonitrile, 12-epi-hapalindole E, 12-epi-fischerindole U isonitrile, fischerindole L, 12-epi-fischerindole I isonitrile, welwitindolinone A isonitrile, welwitindolinone B isothiocyanate, welwitindolinone C isothiocyanate, N-methylwelwitindolinone C isothiocyanate, N-methylwelwitinsolinone C isonitrile, 3-epi-welwitindolinone B isothiocyanate, 3-(Z-2'-isocyanoethenyl)-indole	Hapalosiphon welwitschii UTEX B 1830
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000643.3	0.36	terpene	carotenoid	Brevundimonas vesicularis
BGC0001605.3	0.36	terpene	gibberellin	Sinorhizobium fredii NGR234

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

BHS07_RS23960: PT_FPPS_like

Domain 1 (36 - 296): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 21.84 out of 21.84

Confidence: strong

Additional weak hits (4):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.71 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.76 out of 21.55

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.10 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
MexT	Global virulence regulator

NZ_CP017172.1 - Region 21 - RiPP-like

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Location: 6,315,526 - 6,326,686 nt. (total: 11,161 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002115.3	0.42	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0002013.4	0.33	ribosomal	curacozole	Streptomyces curacoi
BGC0000615.4	0.23	ribosomal	TP-1161	Nocardiopsis sp. TFS65-07
BGC0001224.5	0.23	other (other)	reductasporine	uncultured bacterium
BGC0001753.3	0.23	ribosomal	radamycin, globimycin	Streptomyces globisporus subsp. globisporus
BGC0002482.2	0.21	other (other)	tjipanazole D, tjipanazole I, tjipanazole L, tjipanazole K, tjipanazole M	Symphyonema bifilamentata 97.28

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002115.3	0.42	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0002013.4	0.33	ribosomal	curacozole	Streptomyces curacoi
BGC0000615.4	0.23	ribosomal	TP-1161	Nocardiopsis sp. TFS65-07
BGC0001224.5	0.23	other (other)	reductasporine	uncultured bacterium
BGC0001753.3	0.23	ribosomal	radamycin, globimycin	Streptomyces globisporus subsp. globisporus
BGC0002482.2	0.21	other (other)	tjipanazole D, tjipanazole I, tjipanazole L, tjipanazole K, tjipanazole M	Symphyonema bifilamentata 97.28

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.28 out of 19.52

Confidence: medium

Additional weak hits (3):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.85 out of 32.74

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.74 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.84 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production

NZ_CP017172.1 - Region 22 - RiPP-like

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Location: 6,346,858 - 6,357,703 nt. (total: 10,846 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000979.3	0.38	NRPS, PKS	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980.3	0.38	NRPS, PKS	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0002665.2	0.33	ribosomal	3-thiaglutamate	Pseudomonas syringae pv. maculicola str. ES4326
BGC0000467.5	0.32	NRPS	yersiniabactin	Yersinia enterocolitica
BGC0001486.4	0.32	ribosomal	3-thiaglutamate	Pseudomonas syringae pv. maculicola str. ES4326
BGC0000043.5	0.29	PKS	curacin A	Moorea producens 19L
BGC0001051.5	0.28	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0002711.2	0.28	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0001404.3	0.28	PKS	sorbicillin	Penicillium rubens Wisconsin 54-1255
BGC0001109.4	0.28	NRPS, PKS	pederin	unidentified unclassified sequences.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000979.3	0.38	NRPS, PKS	cylindrospermopsin	Aphanizomenon sp. 10E9
BGC0000980.3	0.38	NRPS, PKS	cylindrospermopsin	Aphanizomenon sp. 22D11
BGC0002665.2	0.33	ribosomal	3-thiaglutamate	Pseudomonas syringae pv. maculicola str. ES4326
BGC0000467.5	0.32	NRPS	yersiniabactin	Yersinia enterocolitica
BGC0001486.4	0.32	ribosomal	3-thiaglutamate	Pseudomonas syringae pv. maculicola str. ES4326
BGC0000043.5	0.29	PKS	curacin A	Moorea producens 19L
BGC0001051.5	0.28	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0002711.2	0.28	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0001404.3	0.28	PKS	sorbicillin	Penicillium rubens Wisconsin 54-1255
BGC0001109.4	0.28	NRPS, PKS	pederin	unidentified unclassified sequences.

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (6):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.04 out of 31.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.56 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.95 out of 29.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.15 out of 29.25

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.58 out of 26.12

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.32 out of 30.98

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_CP017172.1 - Region 23 - terpene

Download region SVG

Location: 7,777,086 - 7,798,471 nt. (total: 21,386 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BHS07_RS32155: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001198.3	0.56	terpene	(+)-O-methylkolavelool	Herpetosiphon aurantiacus DSM 785
BGC0001610.5	0.29	other (other)	gougerotin	Streptomyces ahygroscopicus subsp. wuzhouensis
BGC0000120.5	0.28	PKS	patulin	Penicillium expansum
BGC0001084.5	0.26	NRPS, other (other), terpene	notoamide A	Aspergillus sp. MF297-2
BGC0001762.3	0.23	other (other)	rubrolone A, rubrolone B	Streptomyces sp. KIB-H033
BGC0001225.5	0.23	NRPS	celesticetin	Streptomyces caelestis
BGC0001199.5	0.22	PKS	akaeolide	Streptomyces sp. NBRC 109706
BGC0000150.4	0.21	NRPS, PKS	sporolide A, sporolide B	Salinispora tropica CNB-440
BGC0001073.5	0.21	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0001370.4	0.21	NRPS	lipopeptide 8D1-1, lipopeptide 8D1-2	Streptomyces rochei

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001198.3	0.56	terpene	(+)-O-methylkolavelool	Herpetosiphon aurantiacus DSM 785
BGC0001610.5	0.29	other (other)	gougerotin	Streptomyces ahygroscopicus subsp. wuzhouensis
BGC0000120.5	0.28	PKS	patulin	Penicillium expansum
BGC0001084.5	0.26	NRPS, other (other), terpene	notoamide A	Aspergillus sp. MF297-2
BGC0001762.3	0.23	other (other)	rubrolone A, rubrolone B	Streptomyces sp. KIB-H033
BGC0001225.5	0.23	NRPS	celesticetin	Streptomyces caelestis
BGC0001199.5	0.22	PKS	akaeolide	Streptomyces sp. NBRC 109706
BGC0000150.4	0.21	NRPS, PKS	sporolide A, sporolide B	Salinispora tropica CNB-440
BGC0001073.5	0.21	NRPS, PKS	kosinostatin	Micromonospora sp. TP-A0468
BGC0001370.4	0.21	NRPS	lipopeptide 8D1-1, lipopeptide 8D1-2	Streptomyces rochei

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

BHS07_RS32115: T2TS

Domain 1 (141 - 446): Type 2 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (7):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.37 out of 18.57

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.52 out of 18.57

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.88 out of 30.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.89 out of 33.73

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.21 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 23.12 out of 36.53

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_CP017172.1 - Region 24 - indole

Download region SVG

Location: 7,854,163 - 7,877,174 nt. (total: 23,012 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000829.5	0.70	other (other)	violacein	Chromobacterium violaceum
BGC0000830.5	0.70	other (aminocoumarin)	violacein	uncultured bacterium
BGC0000831.3	0.68	other (other)	violacein	Duganella sp. B2
BGC0000817.4	0.59	other (other)	methylarcyriarubin	uncultured bacterium
BGC0001336.5	0.42	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0001224.5	0.42	other (other)	reductasporine	uncultured bacterium
BGC0001873.3	0.39	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001223.5	0.36	other (other)	hydroxysporine	uncultured bacterium
BGC0000821.5	0.34	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0001333.5	0.34	other (other)	BE-54017	uncultured bacterium AB1650

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000829.5	0.70	other (other)	violacein	Chromobacterium violaceum
BGC0000830.5	0.70	other (aminocoumarin)	violacein	uncultured bacterium
BGC0000831.3	0.68	other (other)	violacein	Duganella sp. B2
BGC0000817.4	0.59	other (other)	methylarcyriarubin	uncultured bacterium
BGC0001336.5	0.42	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0001224.5	0.42	other (other)	reductasporine	uncultured bacterium
BGC0001873.3	0.39	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001223.5	0.36	other (other)	hydroxysporine	uncultured bacterium
BGC0000821.5	0.34	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0001333.5	0.34	other (other)	BE-54017	uncultured bacterium AB1650

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (8):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.88 out of 31.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.65 out of 21.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.93 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.38 out of 29.38

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.74 out of 27.18

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.09 out of 23.43

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.54 out of 29.38

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.01 out of 32.93

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_CP017172.1 - Region 25 - terpene

Download region SVG

Location: 7,953,179 - 7,975,446 nt. (total: 22,268 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

BHS07_RS32780: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0000657.5	0.53	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0001964.2	0.27	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001457.4	0.24	NRPS	jomthonic acid A, jomthonic acid B, jomthonic acid C	Streptomyces caniferus
BGC0000653.5	0.23	terpene	pentalenolactone	Streptomyces arenae
BGC0002642.2	0.22	other (other)	clipibycyclene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000262.5	0.22	PKS, saccharide	prejadomycin, rabelomycin, gaudimycin C, gaudimycin D, UWM6, gaudimycin A	Streptomyces sp. PGA64
BGC0002426.3	0.21	NRPS	deschlorosvetamycin A, svetamycin H, svetamycin A	Streptomyces sp.
BGC0002666.2	0.19	NRPS, other (other)	Legonmycin A, Legonmycin B, Legonindolizine A, Legonindolizine B	Streptomyces sp. MA37

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0000657.5	0.53	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0001964.2	0.27	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001457.4	0.24	NRPS	jomthonic acid A, jomthonic acid B, jomthonic acid C	Streptomyces caniferus
BGC0000653.5	0.23	terpene	pentalenolactone	Streptomyces arenae
BGC0002642.2	0.22	other (other)	clipibycyclene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000262.5	0.22	PKS, saccharide	prejadomycin, rabelomycin, gaudimycin C, gaudimycin D, UWM6, gaudimycin A	Streptomyces sp. PGA64
BGC0002426.3	0.21	NRPS	deschlorosvetamycin A, svetamycin H, svetamycin A	Streptomyces sp.
BGC0002666.2	0.19	NRPS, other (other)	Legonmycin A, Legonmycin B, Legonindolizine A, Legonindolizine B	Streptomyces sp. MA37

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

BHS07_RS32780: T1TS + T1TS

Domain 1 (6 - 337): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (338 - 715): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.90 out of 20.97

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.25 out of 28.19

Confidence: medium

Additional weak hits (12):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.54 out of 25.75

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 21.34 out of 29.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.51 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.48 out of 29.38

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.62 out of 26.12

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.06 out of 29.38

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.23 out of 27.55

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.91 out of 39.62

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.37 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.64 out of 26.06

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.79 out of 20.97

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.04 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
ZuR variant 1	Zinc-responsive repressor

NZ_CP017172.1 - Region 26 - HR-T2PKS,lanthipeptide-class-ii

BGC0000563.4 (AEA03263.1):

Download region SVG

Location: 8,115,367 - 8,171,934 nt. (total: 56,568 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)
HR-T2PKS: (hr-t2pks-ksa and (t2fas or APE_KS2 or ketoacyl-synt))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BHS07_RS33530: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

BHS07_RS33610: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BHS07_RS33615: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acetyltransferase aminotransferase

BHS07_RS33525: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

BHS07_RS33535: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

BHS07_RS33550: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Hydrolases: hydrolase

BHS07_RS33590: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

BHS07_RS33650: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: cyclase

BHS07_RS33495: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: synthase

BHS07_RS33595: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS33600: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS33620: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BHS07_RS33625: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.37	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002703.2	0.32	ribosomal	archalan α	Halorussus salinus
BGC0000859.3	0.32	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000855.3	0.32	other (other)	ectoine	Methylomicrobium kenyense
BGC0002704.2	0.32	ribosomal	archalan β	Halorussus salinus
BGC0000856.3	0.32	other (other)	ectoine	Methylophaga thalassica
BGC0000554.4	0.31	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001579.4	0.30	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000919.5	0.29	other (other)	phaseolotoxin	Pseudomonas syringae
BGC0000857.3	0.29	other (other)	ectoine	Methylophaga alcalica

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000866.5	0.61	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0002192.3	0.59	PKS	FR901512	fungal sp. No.14919
BGC0001258.3	0.56	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002154.2	0.56	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.56	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001987.2	0.53	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0000867.3	0.52	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002510.2	0.51	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0001197.5	0.51	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002047.2	0.50	PKS	brasiliquinone A, brasiliquinone B	Nocardia brasiliensis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BHS07_RS33495

allorf_8116757_8116927

Leader:

MPLRLDPGLRGSALRNRALSRTRWMHEAASEAAA

Core:

PTCLRPVRQRAAHQVATHTADA

Core with dehydrations:

PDhbCysLRPVRQRAAHQVADhbHDhbADA

antiSMASH-DB 4.0 matches for allorf_8116757_8116927

100.0%

NZ_CP017172.1and 1 other:

NZ_CP017173.1 Lanthipeptides: allorf_7993653_7993823_lanthipeptide

Lanthipeptides: allorf_8116757_8116927_lanthipeptide

1

PTCLRPVRQRAAHQVATHTADA

22

PTCLRPVRQRAAHQVATHTADA

1

PTCLRPVRQRAAHQVATHTADA

22

95.5%

NZ_CP017170.1and 1 other:

NZ_CP113950.1 Lanthipeptides: allorf_8163607_8163777_lanthipeptide

Lanthipeptides: allorf_7960164_7960334_lanthipeptide

1

PTCLRPVRQRAAHQVATHTADA

22

PTCLRPVRQRAAHQVATHTA+A

1

PTCLRPVRQRAAHQVATHTANA

22

22.2%

NZ_CP017599.1:

Lanthipeptides: allorf_0187184_0187309_lanthipeptide

2

TCLRPVRQRAAH

13

+CL P+ Q AH

5

SCLEPISQPTAH

16

with 5 more sequence matches

20.8%

NZ_CP041604.2:

Lanthipeptides: allorf_01200655_01200861_lanthipeptide

1

PTCLRP------VRQRAAHQVATHTAD

21

P CL P R R H V T T D

24

PVCLAPWTPTSRTRMRPIHAVPTTTGD

50

20.8%

NZ_CP041610.2and 1 other:

NZ_CP041650.2 Lanthipeptides: allorf_01115720_01115926_lanthipeptide

Lanthipeptides: allorf_00762916_00763122_lanthipeptide

1

PTCLRP------VRQRAAHQVATHTAD

21

P CL P R R H V T T D

24

PVCLAPWTPTSRTRMRPIHAVPTATGD

50

20.8%

NZ_CP041611.1:

Lanthipeptides: allorf_00812896_00813102_lanthipeptide

1

PTCLRP------VRQRAAHQVATHTAD

21

P CL P R R H V T T D

24

PVCLAPWMPTSRARMRPFHAVPTATGD

50

20.8%

NZ_CP041613.2:

Lanthipeptides: allorf_01211022_01211228_lanthipeptide

1

PTCLRPV------RQRAAHQVATHTAD

21

P CL P R R H V T T D

24

PVCLAPWMPTSRPRMRPFHAVPTATGD

50

15.6%

NZ_CP023702.1:

Lanthipeptides: CP967_RS34105_lanthipeptide

3

CLRPVRQRAAHQVAT

17

CL+PV+ A +AT

23

CLKPVQLVAFSGLAT

37

allorf_8116946_8117059

Leader:

MEVTPAPDAMLKGQMRLRPANDLCGG

Core:

ALSPVPCTPGG

Core with dehydrations:

ALDhaPVPCysDhbPGG

antiSMASH-DB 4.0 matches for allorf_8116946_8117059

100.0%

NZ_CP017170.1and 3 others:

NZ_CP017172.1 Lanthipeptides: allorf_8116946_8117059_lanthipeptideNZ_CP017173.1 Lanthipeptides: allorf_7993842_7993955_lanthipeptideNZ_CP113950.1 Lanthipeptides: allorf_8163796_8163909_lanthipeptide

Lanthipeptides: allorf_7960353_7960466_lanthipeptide

1

ALSPVPCTPGG

11

ALSPVPCTPGG

1

ALSPVPCTPGG

11

81.8%

NZ_CP017169.1:

Lanthipeptides: allorf_8034138_8034251_lanthipeptide

1

ALSPVPCTPGG

11

ALSPVPC GG

1

ALSPVPCASGG

11

20.0%

NZ_CP096986.1and 1 other:

NZ_CP096988.1 Lanthipeptides: CLAUR_RS14715_lanthipeptide

Lanthipeptides: CLROS_RS20470_lanthipeptide

1

ALSPVPCTPG

10

A S V CTPG

2

ATSAVACTPG

11

allorf_8119158_8119337

Leader:

MREVGAGLPPSLAECPPSVGSRAVLLFHDARDLSGG

Core:

ECVRFHRVGAAEPLPRSPPGATT

Core with dehydrations:

ECysVRFHRVGAAEPLPRDhaPPGADhbDhb

antiSMASH-DB 4.0 matches for allorf_8119158_8119337

100.0%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: allorf_7962565_7962744_lanthipeptideNZ_CP017172.1 Lanthipeptides: allorf_8119158_8119337_lanthipeptideNZ_CP017173.1 Lanthipeptides: allorf_7996054_7996233_lanthipeptideNZ_CP113950.1 Lanthipeptides: allorf_8166008_8166187_lanthipeptide

Lanthipeptides: allorf_8036350_8036529_lanthipeptide

1

ECVRFHRVGAAEPLPRSPPGATT

23

ECVRFHRVGAAEPLPRSPPGATT

1

ECVRFHRVGAAEPLPRSPPGATT

23

29.0%

NZ_BNBI01000017.1:

Lanthipeptides: allorf_052073_052192_lanthipeptide

4

RFHRVGAAEPLPRSP

18

R R AEPLPR P

16

RAPRSAKAEPLPRGP

30

allorf_8124090_8124275

Leader:

MDMWRAPGGRRHKDCAGG

Core:

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

Core with dehydrations:

RERRDhaRGGLEQARARDhaCysPDhaAPGAVRPVPFDDhbAPVRRHVAMCysRH

antiSMASH-DB 4.0 matches for allorf_8124090_8124275

100.0%

NZ_CP017172.1:

Lanthipeptides: allorf_8124090_8124275_lanthipeptide

1

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

43

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

1

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

43

97.7%

NZ_CP017173.1and 1 other:

NZ_CP113950.1 Lanthipeptides: allorf_8170940_8171125_lanthipeptide

Lanthipeptides: allorf_8000986_8001171_lanthipeptide

1

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

43

RERRSRGGLEQARARSCPSA GAVRPVPFDTAPVRRHVAMCRH

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMCRH

43

95.3%

NZ_CP017170.1:

Lanthipeptides: allorf_7967497_7967682_lanthipeptide

1

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

43

RERRSRGGL+QARARSCPSA GAVRPVPFDTAPVRRHVAMCRH

1

RERRSRGGLKQARARSCPSAHGAVRPVPFDTAPVRRHVAMCRH

43

with 8 more sequence matches

95.3%

NZ_FNAJ01000002.1:

Lanthipeptides: allorf_780201_780386_lanthipeptide

1

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

43

RERRSRGGLEQARARSCPSA GAVRPVPFDTAPVRRHVAM RH

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMGRH

43

86.0%

NZ_CP017171.1:

Lanthipeptides: allorf_7652556_7652741_lanthipeptide

1

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

43

RERRSRGGLEQARARSCPS AVRPVPFDT VRRHVAMC H

1

RERRSRGGLEQARARSCPSVHAAVRPVPFDTGSVRRHVAMCPH

43

61.4%

NZ_CP017169.1:

Lanthipeptides: allorf_8041282_8041509_lanthipeptide

1

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

43

RERRSRGGLEQARARSCPS GAVRPVPFDTAP A CRH

1

RERRSRGGLEQARARSCPSGHGAVRPVPFDTAP---GAAPCRH

40

27.9%

NZ_CP017169.1:

Lanthipeptides: allorf_8041304_8041468_lanthipeptide

28

PFDTAPVRRHVAMCRH

43

P PVRRHVAMCRH

20

PSTPPPVRRHVAMCRH

35

23.2%

NZ_FNPT01000006.1:

Lanthipeptides: allorf_214830_215027_lanthipeptide

7

GGLEQARARSCPSAPGAVRPVPFDTAPVR

35

GL+ ARARS G R P D A +R

16

AGLDDARARS-----GLRRSAPPDAACIR

39

18.6%

NZ_MKZR01000001.1:

Lanthipeptides: allorf_3001482_3001715_lanthipeptide

9

LEQARARSCPSAPGAVRPVPF

29

L A R C + AV+ PF

3

LRTAINRYCLNTESAVKQYPF

23

14.0%

NZ_CP021707.1:

Lanthipeptides: allorf_3956612_3956845_lanthipeptide

17

CPSAPGAVRPVP

28

C APG +P+P

8

CSEAPGRTQPIP

19

11.6%

NZ_NVEA01000022.1:

Lanthipeptides: COL15_RS32130_lanthipeptide

10

EQARARSCPSAPGAVR

25

E+ +CPS P ++

13

EEFNLYTCPSTPCTIQ

28

allorf_8125206_8125337

Leader:

MSESSCGVSVIGLPNAIVALPSVRMPVVLAG

Core:

RGGDDGFCPSRA

Core with dehydrations:

RGGDDGFCysPDhaRA

antiSMASH-DB 4.0 matches for allorf_8125206_8125337

100.0%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: allorf_7968613_7968744_lanthipeptideNZ_CP017172.1 Lanthipeptides: allorf_8125206_8125337_lanthipeptideNZ_CP017173.1 Lanthipeptides: allorf_8002102_8002233_lanthipeptideNZ_CP113950.1 Lanthipeptides: allorf_8172056_8172187_lanthipeptide

Lanthipeptides: allorf_8042399_8042530_lanthipeptide

1

RGGDDGFCPSRA

12

RGGDDGFCPSRA

1

RGGDDGFCPSRA

12

41.2%

NZ_AUAX01000004.1:

Lanthipeptides: allorf_216367_216588_lanthipeptide

2

GGDDGFCPSRA

12

GG G C SRA

6

GGQRGTCKSRA

16

20.0%

NZ_AP021901.1:

Lanthipeptides: allorf_0936478_0936618_lanthipeptide

6

GFCPSRA

12

G+C SRA

1

GYCSSRA

7

allorf_8132558_8132767

Leader:

MRSWRLELRSRGPSPGRNTHPHERLTRSTSPAA

Core:

FPRGASARLDSPRTPTVHTAPCWCLGLGLGLKGNPV

Core with dehydrations:

FPRGADhaARLDDhaPRDhbPDhbVHDhbAPCysWCysLGLGLGLKGNPV

antiSMASH-DB 4.0 matches for allorf_8132558_8132767

100.0%

NZ_CP017172.1:

Lanthipeptides: allorf_8132558_8132767_lanthipeptide

1

FPRGASARLDSPRTPTVHTAPCWCLGLGLGLKGNPV

36

FPRGASARLDSPRTPTVHTAPCWCLGLGLGLKGNPV

1

FPRGASARLDSPRTPTVHTAPCWCLGLGLGLKGNPV

36

22.9%

NZ_CP071090.1:

Lanthipeptides: allorf_11064180_11064347_lanthipeptide

9

LDSPRTPTVHTAPCWCL-GLGLGLKGNP

35

+DS R T + C CL + L + GNP

21

IDSMRVSTWKSQECNCLFSVTLRVSGNP

48

22.2%

NZ_CP016076.1:

Lanthipeptides: UA74_RS09540_lanthipeptide

6

SARLDSPRTPTVHTAPCWCL

25

SAR+ P T P WC+

4

SARICGPTATLTLTLPAWCI

23

with 1 more sequence matches

14.0%

NZ_BNAY01000001.1:

Lanthipeptides: allorf_1318663_1318878_lanthipeptide

8

RLDSPRTPTVHTAPCWC

24

R PR+P VH P C

27

RSRRPRSPAVHEGPLAC

43

MIBiG 4.0 matches for allorf_8132558_8132767

13.9%

Lanthipeptide: venezuelin

20

APCWCLGL

27

A C C+GL

1

ATCECVGL

8

13.9%

BGC0000582.4 (ADR72962.1):

Linaridin: cypemycin

11

SPRTPTV

17

+P TPTV

2

TPATPTV

8

allorf_8132949_8133116

Leader:

MGRPEGPSSAGA

Core:

SSQSSRAPRLFRPNPARVSSRPWGRRSVHGLLRGLVCADTRAS

Core with dehydrations:

DhaDhaQDhaDhaRAPRLFRPNPARVDhaDhaRPWGRRDhaVHGLLRGLVCysADDhbRADha

antiSMASH-DB 4.0 matches for allorf_8132949_8133116

100.0%

NZ_CP017169.1and 1 other:

NZ_CP017172.1 Lanthipeptides: allorf_8132949_8133116_lanthipeptide

Lanthipeptides: allorf_8050142_8050309_lanthipeptide

1

SSQSSRAPRLFRPNPARVSSRPWGRRSVHGLLRGLVCADTRAS

43

SSQSSRAPRLFRPNPARVSSRPWGRRSVHGLLRGLVCADTRAS

1

SSQSSRAPRLFRPNPARVSSRPWGRRSVHGLLRGLVCADTRAS

43

97.7%

NZ_CP017170.1and 2 others:

NZ_CP017173.1 Lanthipeptides: allorf_8007609_8007776_lanthipeptideNZ_CP113950.1 Lanthipeptides: allorf_8179799_8179966_lanthipeptide

Lanthipeptides: allorf_7976356_7976523_lanthipeptide

1

SSQSSRAPRLFRPNPARVSSRPWGRRSVHGLLRGLVCADTRAS

43

SSQSSRAPRLFRPNPARVSSRPWGRRSV GLLRGLVCADTRAS

1

SSQSSRAPRLFRPNPARVSSRPWGRRSVRGLLRGLVCADTRAS

43

27.9%

NZ_CP029710.1:

Lanthipeptides: DMB37_RS40355_lanthipeptide

17

RVSSRP--WGRRSVHGLLRGLVCADTRAS

43

+V P W GLL GLV A T S

3

QVGGNPTTWSAECAAGLLAGLVAALTTGS

31

with 4 more sequence matches

27.9%

NZ_CP096658.1:

Lanthipeptides: allorf_3189783_3190004_lanthipeptide

2

SQSSRAPRLFRPNPARVSSRPWGRRSVH

29

S+S R P FRP R+S RRS

3

SRSGRCPAAFRPGVRRLSPSRDARRSCQ

30

27.9%

NZ_CP113837.1:

Lanthipeptides: allorf_4189826_4189987_lanthipeptide

4

SSRAPRLFRPNPARVSSRPWGRRSV

28

S R R P P RVSSR W + +V

9

SCRQQRECSPEPLRVSSRSWHQSAV

33

18.6%

NZ_CP010407.1and 2 others:

NZ_CP010407.1 Lanthipeptides: allorf_8464166_8464387_lanthipeptideNZ_CP010407.1 Lanthipeptides: allorf_8464166_8464387_lanthipeptide

Lanthipeptides: allorf_8464166_8464387_lanthipeptide

25

RRSVHGLLRGLVCADT

40

+RSV ++GLVC T

1

KRSVDCSMKGLVCCRT

16

18.6%

NZ_QOIL01000028.1:

Lanthipeptides: allorf_043096_043206_lanthipeptide

17

RVSSRPWGRRSVH

29

R + RPW RRS H

1

RATGRPWCRRSDH

13

MIBiG 4.0 matches for allorf_8132949_8133116

11.6%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

27

SVHGLLRGLV

36

+V GL+ G+V

4

AVTGLILGIV

13

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.90 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.90 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.38 out of 23.79

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.74 out of 29.72

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

Additional weak hits (4):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.79 out of 28.14

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.76 out of 30.25

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.04 out of 20.01

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

TFBS Finder

Core peptide predictions

allorf_8116757_8116927 - Class II

Cleavage pHMM score: -1.30

RODEO score: 0

Monoisotopic mass: 2344.2 Da

Molecular weight: 2345.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2363.7 Da N = 1; 2381.7 Da N = 2

allorf_8116946_8117059 - Class II

Cleavage pHMM score: -5.60

RODEO score: 0

Monoisotopic mass: 961.5 Da

Molecular weight: 962.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 980.1 Da N = 1

allorf_8119158_8119337 - Class II

Cleavage pHMM score: -4.10

RODEO score: 0

Monoisotopic mass: 2393.2 Da

Molecular weight: 2394.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2412.7 Da N = 1; 2430.7 Da N = 2

allorf_8124090_8124275 - Class II

Cleavage pHMM score: -3.40

RODEO score: 0

Monoisotopic mass: 4720.5 Da

Molecular weight: 4723.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 4741.4 Da N = 1; 4759.4 Da N = 2

allorf_8125206_8125337 - Class II

Cleavage pHMM score: -4.90

RODEO score: 0

Monoisotopic mass: 1218.5 Da

Molecular weight: 1219.3 Da

Number of bridges: 1

allorf_8132558_8132767 - Class II

Cleavage pHMM score: -4.70

RODEO score: 0

Monoisotopic mass: 3654.0 Da

Molecular weight: 3656.2 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3674.2 Da N = 1; 3692.3 Da N = 2; 3710.3 Da N = 3

allorf_8132949_8133116 - Class II

Cleavage pHMM score: -1.70

RODEO score: 0

Monoisotopic mass: 4581.5 Da

Molecular weight: 4584.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4602.2 Da N = 1; 4620.2 Da N = 2; 4638.2 Da N = 3; 4656.2 Da N = 4; 4674.3 Da N = 5; 4692.3 Da N = 6; 4710.3 Da N = 7; 4728.3 Da N = 8

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C

NZ_CP017172.1 - Region 27 - T3PKS

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Location: 8,402,958 - 8,444,040 nt. (total: 41,083 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase reductase

BHS07_RS34645: alkyl hydroperoxide reductase/ Thiol specific

smcogs: SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific

BHS07_RS34685: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BHS07_RS34700: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

Transferases: methyltransferase

BHS07_RS34670: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

BHS07_RS34715: isoprenylcysteine carboxyl methyltransferase

smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

BHS07_RS34830: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: ligase synthase

BHS07_RS34705: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BHS07_RS34720: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001831.3	0.93	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0000282.3	0.75	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001310.5	0.54	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0001964.2	0.53	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001962.3	0.50	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0002733.3	0.47	other (other)	sinapigladioside	Burkholderia gladioli
BGC0000916.5	0.45	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0002332.2	0.44	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001831.3	0.93	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0000282.3	0.75	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001310.5	0.54	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0001964.2	0.53	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001962.3	0.50	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0002733.3	0.47	other (other)	sinapigladioside	Burkholderia gladioli
BGC0000916.5	0.45	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0002332.2	0.44	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.33 out of 19.52

Confidence: strong

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.77 out of 29.72

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.07 out of 20.47

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.63 out of 28.19

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.33 out of 19.52

Confidence: strong

Additional weak hits (20):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.13 out of 39.62

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.85 out of 20.01

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.92 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.94 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.85 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.44 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.43 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.78 out of 28.14

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.80 out of 29.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.81 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.18 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.07 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.33 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.38 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.02 out of 26.06

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.20 out of 26.12

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.80 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.87 out of 20.01

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

BHS07_RS34705: NH2

CAL_domain (49..484): NH2

minowa: NH2

Prediction, score:

NH2: 91.1
Acetyl-CoA: 17.5
AHBA: 9.8
fatty_acid: 5.1
shikimic_acid: 0.0