LOCUS       NZ_CP017172            10846 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Myxococcus xanthus strain KF4.3.9c1 chromosome, complete genome.
ACCESSION   NZ_CP017172
VERSION     NZ_CP017172.1
KEYWORDS    RefSeq.
SOURCE      Myxococcus xanthus
  ORGANISM  Myxococcus xanthus
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Myxococcus.
REFERENCE   1  (bases 1 to 9429999)
  AUTHORS   Wielgoss,S., Wolfensberger,R., Sun,L., Fiegna,F. and Velicer,G.J.
  TITLE     Social genes are selection hotspots in kin groups of a soil microbe
  JOURNAL   Science 363 (6433), 1342-1345 (2019)
   PUBMED   30898932
REFERENCE   2  (bases 1 to 9429999)
  AUTHORS   Wielgoss,S. and Velicer,G.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2016) Institute of Integrative Biology, ETH
            Zurich, Universitaetstrasse 16, Zurich 8092, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Coverage                   :: 25x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 02:13:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,783
            CDSs (total)                      :: 7,699
            Genes (coding)                    :: 7,611
            CDSs (with protein)               :: 7,611
            Genes (RNA)                       :: 84
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 29 of 88
            Pseudo Genes (incomplete)         :: 73 of 88
            Pseudo Genes (internal stop)      :: 6 of 88
            Pseudo Genes (multiple problems)  :: 19 of 88
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:23:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6346857
            Orig. end                         :: 6357703
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017172.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source strain/DNA available from Prof. Gregory Velicer, IBZ, ETH
            Zurich, Universitaetstrasse 16, 8092 Zurich, Switzerland.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10846
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="22"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10846
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10846
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5846](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5846)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            326..1450
                     /locus_tag="BHS07_RS25970"
                     /old_locus_tag="BHS07_25340"
     CDS             326..1450
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554930.1"
                     /locus_tag="BHS07_RS25970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25340"
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="WP_140794021.1"
                     /transl_table=11
                     /translation="MRSTFHIAVATLLACCAAGCGNLENAPFRVGTVHGQLTESDPSVA
                     MVSLVDQPGVSSHVDADGRFTLEDIPTGMAELFIVATAEKAARVQVQVLGGQSVQVQPV
                     APTPAGFLDLRVKTTNGFRLSAAEVSVKGTPFQRLLLDAKGRLRVGPLPDGCYTVTVTA
                     LGFPATQVEDCAGPGEKKELKVDLEADESLLEQGCQEIGCEEGLVCAPNKKCLECFGNS
                     QCGAGLTCKGNRCEGPGPLCAPCTGDWQCAAGTHCEVLPEGSAACVARCGDDDDAPPTA
                     QALSVDDYSARCAPGFTCQSGRCLPDAANFAGCHALRRMDAPCTDDANCHELGLPGGRC
                     VSGACTVPCATDRDCPDTRWCVTSSAGRVCQVGT"
     gene            1455..4244
                     /locus_tag="BHS07_RS25975"
                     /old_locus_tag="BHS07_25345"
     CDS             1455..4244
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554931.1"
                     /locus_tag="BHS07_RS25975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25345"
                     /product="hypothetical protein"
                     /protein_id="WP_140876411.1"
                     /transl_table=11
                     /translation="MASQGTPRLRLILVTLYTVTTWGCEPAPRHPSTVHNTPPRLEVSP
                     TLDITPLRVGQAVEFVASVEDAEDGAALGANVLWVSSVEGQLARGARATLAFSEPGEQR
                     LTASVVDAGGLAASASLHLNVLARGAPVATVLRPAPGSTFNLGEVLGLECVAMTERGAR
                     LQGRAVQWTSALSGPLPAGDVVRATLSVAGEDSLRCTAVDSATGVSATAAVRVTVRATR
                     APSVRILRPEPAEIFVKQGEAPPHGASVRFAATAQDFHAEGGAGNLDGVVEWRLAPTGR
                     PLGRGPALTHAFTAPGVYTVMARVVDGRGNTASDSVQVRLVTNLPPRCELELPTTENPR
                     LLVGVPSRLRARCVDPETEQPVEPTWTTSAEPTPLGTGEAVSAVLSVAGAQVLSACAVD
                     PEDTELRGCVERPVRAIVNTPPLGCAIQAPLPDAVVNAGVPLTLTGSASDAEDARTGLR
                     YTWVSSRDGALAQGPSTHTVRLSTPGHHVLTLTATDPWGLACAATVAVTVNGAPDVRVE
                     SVLQGVLDCGRGPCLEGQPVFLTGFARPAAPQGHIAELTWLDNLAGSLLAVALPQPTAT
                     LALPLAGRHTTVLAATDQNGAVSRAATSFTVLPLGGARLMEDVPDAQRPVVALALTPEG
                     LRYADGASPRVYAERASTTVLDVDAPALSLHAFQGGDGNVLFVGTDGGGVLRCVQHTCT
                     RHTGAMLGATGAAVTAVAAWESPDVLLLGTRRGVVLTRASDPASGGRMGTVGGRRLLEG
                     REVRQLAISPDSSPERVTAWAATSGGLAEITILADDDFEPGLAEVVVRLHTPPAILDDD
                     VLSVAVGPEGQVFAGTRKGFSALGQPGPALRDAPWGLQDEEIRALHFERQTVDARTRDI
                     LWAGTKNGLVRYDVSADIATHFGPEEGLPDPDVSALLVAPGGPRYVGTARGLVTYPWH"
     gene            complement(4267..5004)
                     /locus_tag="BHS07_RS25980"
                     /old_locus_tag="BHS07_25350"
     CDS             complement(4267..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637557.1"
                     /locus_tag="BHS07_RS25980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25350"
                     /product="DNA-binding domain-containing protein"
                     /protein_id="WP_140876413.1"
                     /transl_table=11
                     /translation="MKPSLRDFFDSMGSYLAEPGPESLAALYARHPGWEAPRERVSLYG
                     GFVLGHVRSALDKVFPLTRQALGQDAWDGLVAAFTRKRPARHHELNHLCEDFPAFLADA
                     AASRGLAPFVPALARFEWTDFAVFASLEPSPERVERLTANPTLVVLEHDFHLCAYVRAR
                     GATTSPASGAELALLWRHPEQLKTLYMAATPPALLVLKMAVEGLALADVVAATGMAEAD
                     LRATVARFGRDGLVLLPGDGSGD"
     gene            complement(5001..5846)
                     /locus_tag="BHS07_RS25985"
                     /old_locus_tag="BHS07_25355"
     CDS             complement(5001..5846)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350979.1"
                     /locus_tag="BHS07_RS25985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25355"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_140876415.1"
                     /sec_met_domain="DUF692 (E-value: 3.5e-94, bitscore: 313.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTYAQRHGLKPLGAGIGLRRSFYGELPRTGRTLDWVELIPENFL
                     SLGGRSQSALDACAERWPVLPHGVGLNIGGPDALDDDYVRGLSALVKRVDAPFFSDHLC
                     FSRLDGVYLHDLLPLPFSEAAVEHVVPRVREVMDRVGRPFLVENPSYYARMPGGTLREA
                     DFLRHVVEQADCGLLLDVNNVYVNARNHGYDARAFVDALPLERVVQIHLAGHTCYPDVI
                     VDTHGDRVCDDVWSLYRYVLARTGPVATLIEWDQDIPSLDAVLDEADVARAALREVAGR
                     "
     gene            complement(5961..6206)
                     /locus_tag="BHS07_RS25990"
                     /old_locus_tag="BHS07_25360"
     CDS             complement(5961..6206)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941509.1"
                     /locus_tag="BHS07_RS25990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25360"
                     /product="hypothetical protein"
                     /protein_id="WP_140794029.1"
                     /transl_table=11
                     /translation="MNVKALAAVVGTLSLAALATGCVTKKSAEMEPATAEETGAESSGA
                     TESQEAAPAATEEKGAEGSCGAGSCGSGSCGGQKKE"
     gene            complement(6265..7680)
                     /locus_tag="BHS07_RS25995"
                     /old_locus_tag="BHS07_25365"
     CDS             complement(6265..7680)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637554.1"
                     /locus_tag="BHS07_RS25995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25365"
                     /product="hypothetical protein"
                     /protein_id="WP_237081445.1"
                     /transl_table=11
                     /translation="MRARVAAVILFGLSLMSCQAPLRHHPREDAAALRYNLTYVREPEH
                     AVDVEVVLVLGTPREFLFSQPGAVTTVSVHREDGGVQTLPVQEGRVLLPRGTRFLRYRY
                     ALDASIRERGPDFFQGSGDGDARHVAGRAYLIRPRVVKPGLRVELSLVGTDALLPWQPG
                     ESGVYRLRGEDLVDSGFHGFGGRRCQVRLPDAVLDVAVLGHFTHLSDAAVCTWLGKAAE
                     EVRTVRRAFPHPRITVRVIPAPGHHGPSVFGMVRWSSPPSISISVGQEARPEDFARDWV
                     AVHEMLHLTHPVFVPRVPWLSEGLATYYTEVARARSGRQDSRRAWAELLGGFARGREGN
                     DTRTMQEAVSQDSFSLAVYWTGALFALHLDVELRRITGNTRGLDDVLELLAARGSSSTF
                     GAFGAAVDAVAGQPLFNALLERHLPRPAFAEQAGLLEALGVQDGPNGVSLVPARDSLLR
                     DSVVGRRSHGETP"
     gene            <7992..8363
                     /locus_tag="BHS07_RS26005"
                     /old_locus_tag="BHS07_25370"
                     /pseudo=""
     CDS             <7992..8363
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2fas"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621051.1"
                     /locus_tag="BHS07_RS26005"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25370"
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase II"
                     /pseudo=""
                     /sec_met_domain="t2fas (E-value: 1.6e-42, bitscore: 144.6,
                     seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GYINAPGTSTDLGDALEMEGIARVFGDAARSVAVSSTKSMTGHMN
                     GAAGTAEAIISVLALTRGLLPPTINLERKDPRIQLDCVPHTARPAQVDAVTSNSFGFGS
                     TNVSLVFRRRSPTASSASL"
     misc_feature    8415..8431
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(8568..8583)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.56"
                     /tool="antismash"
     misc_feature    complement(8581..8595)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.95"
                     /tool="antismash"
     misc_feature    8594..8608
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.15"
                     /tool="antismash"
     misc_feature    complement(8602..8614)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.58"
                     /tool="antismash"
     gene            8674..10293
                     /locus_tag="BHS07_RS26010"
                     /old_locus_tag="BHS07_25375"
     CDS             8674..10293
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554936.1"
                     /locus_tag="BHS07_RS26010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25375"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_140794031.1"
                     /transl_table=11
                     /translation="MNDNALNANVGLKLRGLRLARNIKQADAAKDLGVSPAYLNLIEKG
                     KRVMPFPLLWKALRYFDQDPEQFMSTLGEGRVDEALAKLLDEPLLKSLDIDPESLQSLS
                     AEPKLAGTVAALFNLYKNTRTQLENVLAQLNVEERTRAQGGLGNGAAPGVRFDYSPFDE
                     VSDFLEKHRNYFPELEEQADALRRDVRLERQLTSGQLIRLLEERFGYRVLFDDPSPSGS
                     SVVRRLDPEERTLTLSPFLTEQPLKFQIAASIGLLVMDREKLLERVLDAGRMRHGETQR
                     LIKVNLANYFAGALMLPYGDFFKEVQRTRYDVELLSSIFGSTYETVAHRLCNLSDPKRP
                     GLPFHFLRADIAGNISKRYSGTGIRFATGGGSCAKWAVHLAFLNPSQLTRQYSIMPDGT
                     AYFCFAKVQLQPIEGSIVKGTAYSIGLGTHAENAKYLAYGLPTTDLRKDAIPSGISCRF
                     CERTDCNQRAAASYRFAFAFDEYTKKDCFFSPILVHEKADKVEKDRHPGAHSLAVAANG
                     TDGSEKDDSLDKTSRRRKGGDA"
     misc_feature    10740..10756
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.32"
                     /tool="antismash"
ORIGIN
        1 gcagcgaggt gcccttccgc tacaccaccg tctctgccgg agcggccctt ccctacctgt
       61 ggcgctggga gcggctgacg ttgttcgcgg ggccgcgtgt ggccggcctg tacctcaggc
      121 ggtcctttga cgtagaggct gccgccaacg ggcaacggtt cttcaccgtc agccccggcg
      181 tggtgggcgg catcgtgtgg cgggtgggtg agcggctgga gctgatgtca caggcccagt
      241 tcatgctgac gtatgtcgtt gtggatgggc aggggcaggc cgtgggtttc acgggtggct
      301 gggccggcgc gggatatcgt ttctgatgcg ctcgacgttt cacatcgcgg tagccaccct
      361 cctggcctgc tgcgccgcgg gctgcggcaa cctggagaac gcccccttcc gggtgggcac
      421 cgtgcatggg cagctcacgg agagcgaccc ctcggtggcc atggtgtcgc tggtggacca
      481 gcccggcgtg agcagccatg tcgacgcgga cgggcgattc accctggagg acatccccac
      541 ggggatggcg gagctcttca tcgtcgccac ggccgaaaag gccgcccgcg tccaggtgca
      601 ggtgctggga ggccagtccg tccaggtaca accggtggcg cccacgcccg cgggcttcct
      661 cgacctgcgc gtgaagacga ccaacggctt ccggctgtcc gcggcggagg tgtccgtgaa
      721 gggcacgccc ttccagcggc tgctgctgga cgcgaagggg cggctgcgcg tggggcccct
      781 tccggacggc tgctacacgg tgacggtgac ggccctgggc tttccggcca cccaggtcga
      841 ggactgcgcg ggcccaggcg agaagaagga gctcaaggtc gacctggagg cggacgagtc
      901 gctcctcgaa cagggctgcc aagagattgg ctgcgaggaa ggcctggtgt gcgcgccgaa
      961 caagaagtgc ctggagtgct tcggcaactc gcaatgcggc gcgggcctga cgtgcaaggg
     1021 caatcgctgc gagggccccg gtcccctgtg cgcgccctgc acgggcgact ggcaatgcgc
     1081 cgctgggacg cactgcgagg tgctgcccga aggaagcgcc gcgtgcgtcg cccggtgtgg
     1141 cgacgacgat gacgcccccc ccacggccca ggccttgtcg gtcgacgatt actcggcgcg
     1201 gtgcgcgccg ggcttcacct gccagtccgg gcggtgcttg ccagatgccg cgaacttcgc
     1261 gggctgccat gccctgcggc ggatggacgc gccctgcacc gacgacgcga actgccacga
     1321 gctgggactg cccggcgggc gctgtgtcag cggggcctgc acggtgccct gcgcaacgga
     1381 ccgtgactgc ccggacaccc ggtggtgcgt gacttcatcg gcgggccgcg tctgccaggt
     1441 cgggacgtaa caccgtggcg agccaaggca ccccgcggct ccgcctcatc ctggtcaccc
     1501 tgtacaccgt cacgacgtgg ggttgtgagc cggccccccg ccatccttcg acggttcaca
     1561 acacccctcc ccggctcgaa gtcagcccca ccctggacat caccccgctg cgagtgggcc
     1621 aggccgtgga gttcgtggcc tccgtcgagg atgcggagga cggcgccgcg ctgggcgcca
     1681 acgtgctgtg ggtgtcctcc gtcgaagggc aactggcccg cggcgcacgc gccaccctcg
     1741 ccttcagcga accgggagag cagcggctga ccgcctcggt ggtggacgcc ggtggactgg
     1801 ccgcgagcgc gtcactccac ttgaacgtcc tcgcgcgggg cgctcccgtg gccaccgtgc
     1861 tgcgccccgc cccgggcagc accttcaacc tcggcgaggt gttgggcctg gagtgcgtgg
     1921 cgatgacgga gcgtggcgcg cggctccagg ggcgcgcggt gcagtggacg tccgccctgt
     1981 ccgggccgct gcctgccggc gacgtggtgc gggcaaccct gtccgtggcc ggcgaggaca
     2041 gcctccgctg cacggcggtg gactccgcca cgggtgtctc cgccaccgcc gcagtgcgcg
     2101 tcaccgtccg cgccacgcgc gctcccagcg tgcgcatcct ccggcccgag cccgcggaaa
     2161 tcttcgtgaa gcaaggcgag gccccacccc acggcgcctc cgtccgcttc gcggccaccg
     2221 cccaggactt ccacgccgag ggcggcgcgg gcaacctgga cggcgtggtg gagtggcgcc
     2281 tggcgcccac tgggcgtccg ctgggccgag gcccagccct gacacacgcc ttcaccgcgc
     2341 ccggcgtcta cacggtgatg gcgcgggtgg tggatgggcg cggcaacacc gcctcggaca
     2401 gcgtgcaggt gcggctggtc accaaccttc cgccacggtg cgagctggag ctccccacga
     2461 ccgagaatcc acgcctgctc gtaggcgtcc ccagccggct gcgggcccgc tgcgtggacc
     2521 cggagacgga gcaaccggtg gagcccacct ggaccaccag cgccgagccc acgcccctgg
     2581 gcacgggtga agccgtgagc gcagtgctgt ccgtggccgg tgcgcaggtg ttgtcggcgt
     2641 gcgccgtgga ccccgaggac accgagctgc gcggctgtgt ggaacggccg gtgcgcgcca
     2701 tcgtcaacac gccccccctt ggctgcgcca tccaggcacc gcttccggac gcggtggtga
     2761 acgcgggtgt gccgctgacg ctcacgggtt cggcgtcgga cgcggaggat gcgcggacgg
     2821 gcctgcgcta cacgtgggtg tcctcgcggg acggcgccct ggcccagggc ccgagcacgc
     2881 acaccgtccg gctgtccacg cccggccacc acgtgctgac gctcaccgcg acggacccct
     2941 ggggactggc ctgcgcggcg accgtcgccg tcaccgtgaa tggcgcgccc gatgtgcggg
     3001 tggagagcgt gctgcaaggc gtgctggatt gcggcagagg cccatgcctg gaggggcagc
     3061 ccgtgttcct caccggcttc gcgaggcccg cggcccctca ggggcacatc gcggagctga
     3121 cgtggctgga caacctggcg gggagtcttt tggcggtcgc gctgccgcag cccaccgcca
     3181 ccctcgcctt gcccctcgcc ggtcgccaca cgacggtgct ggccgccacc gaccagaacg
     3241 gcgcggtgag ccgcgcggca acgtccttca cggtgctgcc gctgggaggc gcgcggctga
     3301 tggaggacgt gccggacgcg cagcggcccg tggtggccct ggcactcacc ccggaagggc
     3361 tccgctacgc cgacggcgca tcccctcgag tgtatgcgga gcgcgcctcc acgacggtgc
     3421 tggacgtgga cgccccggcc ctgtcgctcc atgcgtttca aggaggagac ggcaacgtgc
     3481 tcttcgtggg caccgacggc ggcggcgtgc tccggtgcgt ccagcacacc tgcacgcgac
     3541 acacgggcgc catgctgggc gccacgggcg cggcggtgac cgcggtggcc gcatgggaat
     3601 ccccggacgt gctgctgctg ggcacccgcc ggggcgtggt cctcacacgc gcgagcgacc
     3661 ccgcctccgg aggacgcatg ggcaccgtgg gcggccgccg cctgctggaa ggacgcgagg
     3721 tgcgccagct cgccatctcc ccggactcca gcccggaacg cgtgacggcc tgggcggcca
     3781 cctcgggtgg gctcgcggaa atcaccatct tggcggacga cgacttcgag cccgggctcg
     3841 ccgaagtcgt ggtgcggctc cacactccac cggccattct cgatgacgac gtcctgtccg
     3901 tggccgtggg gcccgagggc caggtgttcg ccggcacgag gaagggcttc agcgcgctgg
     3961 gacagcccgg tcccgcgctg cgcgacgcgc cctggggact gcaggacgag gagattcgcg
     4021 cgcttcactt cgaacggcag accgtggacg cgcgcacccg ggacatcctc tgggccggca
     4081 cgaagaacgg gctggttcgc tatgacgtgt cagcagacat cgccacccac ttcggaccgg
     4141 aagagggctt gccggacccg gacgtgagcg ccctgctcgt ggcgcccgga ggaccgcgct
     4201 acgtgggcac ggcgcgaggc ctggtgacgt acccctggca ctgagcgccc ggacagcgcg
     4261 ggcccttcaa tccccgctgc cgtcacccgg gaggagcacc aatccatcgc ggccgaagcg
     4321 cgccactgtc gctcgcaggt cggcctccgc catgcccgtg gccgccacca cgtccgccag
     4381 tgccagccct tccaccgcca tcttcagcac cagcagcgcc ggtggcgtgg cggccatgta
     4441 gagcgtcttc aactgctccg ggtggcgcca cagcagcgcc agctccgcgc ccgacgctgg
     4501 tgacgtcgtg gcgcctcggg cccgcacgta cgcgcacagg tggaagtcgt gctccagcac
     4561 caccagggtg ggattggccg tcagccgctc cacgcgttcg ggcgaaggct ccagggacgc
     4621 gaacaccgcg aagtcggtcc actcgaagcg ggccagtgcg ggcacgaagg gcgccaatcc
     4681 ccgcgaggcc gccgcgtccg cgagaaacgc gggaaagtct tcgcacaggt ggttgagctc
     4741 atggtgccgc gcgggccgct tccgcgtgaa ggccgccacc aggccgtccc atgcgtcctg
     4801 gcccagggcc tggcgcgtca gcggaaacac cttgtccagc gcggagcgca catggccgag
     4861 cacgaagccg ccgtagagag acacccgctc acgcggcgcc tcccaccccg ggtgcctcgc
     4921 gtacagcgcc gccagtgact ccgggccggg ctcggcgagg tagctcccca tgctgtcgaa
     4981 gaagtcgcgc agggacggct tcatcggccg gcgacctccc gcagcgcggc tcgcgccaca
     5041 tccgcctcgt ccagcaccgc gtccagggaa ggaatgtcct ggtcccactc gatgagcgtc
     5101 gctaccggac cggtgcgcgc cagcacgtag cggtagagcg accacacgtc gtcgcagaca
     5161 cggtcgccat gcgtgtcgac gatgacgtcc gggtagcagg tgtggccggc gaggtgaatc
     5221 tgcaccacgc gctccagcgg cagggcgtcc acgaaggccc gcgcgtcgta gccatggttg
     5281 cgcgcgttga cgtagacgtt gttcacgtcc agcagcagcc cacagtccgc ctgctccacg
     5341 acgtggcgga ggaaatccgc ctcccgcagc gtgccgccgg gcatccgcgc gtagtagctc
     5401 gggttctcca ctaggaaggg acggcccacg cggtccatca cctcacgcac gcggggcacc
     5461 acgtgctcga cagcggcctc gctgaacggc agcggcagca agtcgtgcag gtacacaccg
     5521 tccagccgcg aaaaacagag gtggtcggag aagaagggcg cgtccacccg cttcaccagc
     5581 gccgacagtc cccggacata gtcgtcatcc agcgcgtccg ggccgccgat gttcaacccc
     5641 acgccgtggg gcaacacggg ccaccgctcc gcgcatgcgt ccagcgcgct ctgcgagcgc
     5701 ccccccagcg acaggaagtt ctccgggatg agctccaccc agtcgagcgt ccgtccggtg
     5761 cgcggcagct ccccatagaa gctcctgcgc agcccgatgc ccgcgcccag cggcttcagc
     5821 ccatgtcgct gtgcgtacgt caccacggta gaagccctct cgaggcgtgg gaaacaaccc
     5881 gggcggcggt ggagctgctc aaccgcccgg caacgagggc ccggccttca ccaggccctc
     5941 gggacttcgg tccggaggaa ctactccttc ttctggccgc cgcaggagcc ggagccgcac
     6001 gagccggcgc cacagctgcc ctcagcgccc ttctcctcgg tcgcggccgg agcggcctcc
     6061 tgcgactccg tggctccgct gctctccgcg ccggtctcct cggccgtggc gggctccatc
     6121 tccgccgact tcttggtcac acaacccgtg gccagcgcgg ccagggacag ggtgccgaca
     6181 accgctgcaa gcgccttgac gttcatggat gagacttcct ttcggtactg cgtggggggt
     6241 gttgtgtgga ggtcgcgggt gcattcaggg tgtttcgccg tggctgcgcc gaccgaccac
     6301 cgaatcacgc aacagactgt ctcgggcggg taccagcgag acgccgttgg gtccgtcctg
     6361 gacacccagc gcttctagca gaccggcctg ttccgcaaaa gcaggccgcg gcaagtgccg
     6421 ctccagcaga gcattgaaca gcggctgtcc cgcgacggca tccaccgcgg caccgaatgc
     6481 cccgaaggtc gaggaactgc cgcgagccgc gagcaactcc agcacgtcat ccagcccgcg
     6541 cgtgtttcca gtgatacggc gcaattccac atccagatgc aacgcgaaga gcgctcccgt
     6601 ccaatagaca gcgagcgaaa agctgtcttg agacacggcc tcctgcatgg ttcgcgtgtc
     6661 attgccctct cgcccccgag cgaagccgcc cagcagttcc gcccaggcgc gccgcgagtc
     6721 ctgccgcccg gagcgcgcac gcgcgacctc cgtgtagtag gtagccagcc cctccgacag
     6781 ccacggcacg cggggcacga agacaggatg ggtcaggtgc agcatctcgt ggacggccac
     6841 ccagtcccgc gcgaagtcct ccggccgcgc ctcctgcccc acgctgatgg agatgctggg
     6901 cggcgagctc cagcgcacca tgccgaagac gctgggcccg tgatgcccag gcgcggggat
     6961 gacacgcacg gtgatgcgcg gatgcgggaa ggcgcgccgc acggtgcgga cttcctccgc
     7021 ggccttgccc aaccaggtgc agacggccgc gtcgctcagg tgcgtgaagt gcccgaggac
     7081 ggccacgtcc agcaccgcgt ccggcaggcg cacctgacac cggcggcctc cgaagccgtg
     7141 gaagcccgag tccaccaggt cctcgccgcg cagccggtac acgccactct ccccgggctg
     7201 ccacggcagc aacgcatcgg tgcccaccag cgacagctcc acgcgcaggc ccggcttcac
     7261 cacccgcggc cggatgaggt acgcgcggcc cgcgacgtgc cgcgcatctc cgtctccgga
     7321 gccctggaag aagtcagggc cccgctcccg gatggaggca tccagcgcgt agcggtagcg
     7381 caagaagcgc gtcccccgag gcagcaagac gcggccctcc tggacgggca acgtctggac
     7441 gcccccatcc tcccggtgca cggacaccgt cgtcacggcg cccggctgac tgaagaggaa
     7501 ctctcgcggc gttcccagca ccagcaccac ctccacatcc accgcgtgct cgggctcacg
     7561 cacgtaggtg aggttgtagc gcagcgccgc cgcatcctcc ctggggtggt ggcgcagtgg
     7621 cgcctgacac gacatcagcg acaggccgaa caggatgacg gcggcgactc gggcacgcat
     7681 gcgcagcggt gccctgctcc acgttcgcgg ccatccatgc ccaccgtcca tacacggtga
     7741 aggttcgccc atgacgcgcc ggaggtgaat ggcgatgagg cctccgggag cgcgggccga
     7801 cacttctcca gcgtcaaccc acactctgtg gcacggcggg gcagcccgag cgtcgtgctc
     7861 agcggggctt cttccgggcg ggagccgcgg ccgggtcacc cggaccagcg gcatggcccc
     7921 gaaacgaagc gccagcgtcg ccagcgcgcg cttgcgcgcc gtgacaccgc cctcccctgg
     7981 cacacgggag cggctacatc aacgcccctg gcacatcgac ggacctgggc gacgccctgg
     8041 agatggaggg cattgcccgc gtcttcgggg acgcggcgcg ctcggtcgcc gtctcttcga
     8101 ccaagtcgat gacgggccac atgaacggcg cggccggcac cgcggaggcc atcatcagcg
     8161 tgctggccct cacccgtggc ctgctgccgc ccaccatcaa cctggagcgg aaggacccgc
     8221 gcatccagct ggactgcgtg ccccacacgg cccgccccgc gcaggtggac gccgtcacga
     8281 gcaactcgtt cggcttcgga agcaccaatg tgtccctggt gttccggcgc cgatcgccca
     8341 cggcctcctc cgccagcctg taaacccacg ctttcgcgtt taccagaacg tgtcccaccc
     8401 atgctgcaat gcatcagtaa atgtttgaca aggcgctttg gagagagagg cggcaggttc
     8461 cccacacgcc ctgaagggca gtgatttcat ggacttgaat gtacccggcg ccctggttcc
     8521 gaccgcaggc cagccggtcc gtcgccgggc cgacaggggt cctcgtggat gaaaacgatc
     8581 attgcaaatc ggtaaatcga tttgcaaagt gggtgaagag cccgaggcgc gcgcggccat
     8641 tcgcgatcgc cgggggcatc aacgcatccg gccatgaacg acaacgcact gaacgccaac
     8701 gtgggcctga agcttcgggg tctgcgccta gcacggaaca tcaaacaggc ggacgcagcc
     8761 aaggacctgg gcgtttcccc cgcgtacctg aacctcatcg agaagggcaa gcgggtgatg
     8821 cccttccccc tgctctggaa ggccctgcgc tacttcgacc aggaccccga gcagttcatg
     8881 tccacgctgg gcgagggccg cgtggacgaa gcgctcgcga agctgctgga cgagccgctc
     8941 ctcaagagcc tcgacatcga cccggagtcg ctccagtccc tctccgcgga gccgaagctc
     9001 gcgggcacgg tggcggcgct cttcaacctc tacaagaaca cccgcacgca gttggagaac
     9061 gtgctggcgc agctcaacgt cgaggagcgc acgcgcgccc agggcggact gggcaacggc
     9121 gccgcgcccg gcgtgcgctt cgactactcg cccttcgacg aggtcagcga cttcctggag
     9181 aagcaccgca actacttccc ggagctggag gagcaggcgg acgcgctgcg ccgcgacgtg
     9241 cgcctggagc ggcagctcac cagcggtcag ctcatccggc tcctggagga gcgcttcggc
     9301 taccgcgtcc tcttcgacga cccgtctccc tccggctcgt ccgtggtgcg gcggttggac
     9361 ccggaggagc ggacgctgac gctgtcgccc ttcctcaccg aacagccgct gaagttccag
     9421 attgccgcgt ccattggcct gctggtgatg gaccgggaga agctgctgga gcgcgtgctg
     9481 gacgcgggcc gcatgcgcca cggcgagacg cagcggctca tcaaggtgaa cctggccaac
     9541 tacttcgccg gcgcgctgat gctgccctac ggggacttct tcaaggaggt ccagcgcacg
     9601 cgctacgacg tggagctgtt gtccagcatc ttcggctcca cctacgagac ggtggcccac
     9661 cgcctgtgca acctgtcgga cccgaagcgc cctgggcttc cgttccactt cctgcgcgcg
     9721 gacatcgccg gcaacatctc caagcgctac agcggcaccg gcatccgctt cgccaccggc
     9781 ggcggcagct gcgccaagtg ggccgtgcac ctggccttcc tcaacccgtc ccagctgacg
     9841 cggcagtact ccatcatgcc ggacggcacc gcctatttct gcttcgccaa ggtgcagctc
     9901 cagccgatcg agggctccat cgtcaagggc accgcctact ccatcggcct gggcacccac
     9961 gcggagaacg ccaagtacct ggcctacggc ctgcccacga cggacctgcg caaggacgct
    10021 attccgtccg gcatctcctg tcgcttctgc gagcgcaccg actgcaacca gcgcgcggcg
    10081 gccagctacc gtttcgcctt cgccttcgac gagtacacga agaaggactg cttcttctcc
    10141 cccatcctcg tccacgagaa ggccgacaag gtggagaagg accggcaccc gggcgcccat
    10201 tccctggccg tggccgccaa cgggactgac gggagcgaga aggacgattc gttggacaag
    10261 acctcccgcc gccgcaaggg tggcgacgcc tgaggccccc catgcaccac ccgcccgaag
    10321 acacccaacg cacccacatc ctcgacgcca tccagaagca gaagaacgcg ctggcgccgc
    10381 tgcgcatcac cggctcgccc accgaagtgg gccagggcct ggtgaacctg gccgaactgc
    10441 acggcctgct ggaggaccac gccgccagcc ggcagttcta cgaagaggcg ctcgagaagt
    10501 tccaggaggc caagtacaag cccggccagg cgcaggccct gatggggctg ggcgtggtga
    10561 aggcgaactt cgaggatcac cgcggcgcca tcgagcagat tgcccgcgcc gccatgctct
    10621 tcaacgagtc caaggaccgt gagggcgagg ccctggcccg cgcctgcatc ggcgagtccc
    10681 tgcgctcgtt gggccagccg gaagccgccg aggagaagta ccaggaggcg ctcatcctcc
    10741 tgcgccagac gcgcaacacg gagcgggtgg cgcggctgct catcgacatt ggcgacatcc
    10801 gcatggagaa gggcgactac gagcccgcgc gcaagcgctt cctgga
//
