LOCUS       NZ_CP017172            11161 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Myxococcus xanthus strain KF4.3.9c1 chromosome, complete genome.
ACCESSION   NZ_CP017172
VERSION     NZ_CP017172.1
KEYWORDS    RefSeq.
SOURCE      Myxococcus xanthus
  ORGANISM  Myxococcus xanthus
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Myxococcus.
REFERENCE   1  (bases 1 to 9429999)
  AUTHORS   Wielgoss,S., Wolfensberger,R., Sun,L., Fiegna,F. and Velicer,G.J.
  TITLE     Social genes are selection hotspots in kin groups of a soil microbe
  JOURNAL   Science 363 (6433), 1342-1345 (2019)
   PUBMED   30898932
REFERENCE   2  (bases 1 to 9429999)
  AUTHORS   Wielgoss,S. and Velicer,G.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2016) Institute of Integrative Biology, ETH
            Zurich, Universitaetstrasse 16, Zurich 8092, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.0
            Genome Coverage                   :: 25x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 02:13:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,783
            CDSs (total)                      :: 7,699
            Genes (coding)                    :: 7,611
            CDSs (with protein)               :: 7,611
            Genes (RNA)                       :: 84
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 29 of 88
            Pseudo Genes (incomplete)         :: 73 of 88
            Pseudo Genes (internal stop)      :: 6 of 88
            Pseudo Genes (multiple problems)  :: 19 of 88
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:23:50
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6315525
            Orig. end                         :: 6326686
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017172.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source strain/DNA available from Prof. Gregory Velicer, IBZ, ETH
            Zurich, Universitaetstrasse 16, 8092 Zurich, Switzerland.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11161
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="21"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11161
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11161
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6161](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6161)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    100..115
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     gene            218..892
                     /locus_tag="BHS07_RS25850"
                     /old_locus_tag="BHS07_25225"
     CDS             218..892
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554906.1"
                     /locus_tag="BHS07_RS25850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25225"
                     /product="hypothetical protein"
                     /protein_id="WP_237079813.1"
                     /transl_table=11
                     /translation="MKPEQEKTLHEEVVATVDRLVLKNPALEKELEQAQGYAVFPSLGR
                     ASLVLGGSYGHGEVFEKGKPIGFATLSQLTLGVQVGGQTLSELIFFKDRDAIEAFKGGK
                     AAFAANASAVIVKAAASGTINYAKCVAHAYSRGGMLLEASLGGQKFTFIPRSSDASERG
                     NGGLVQRFRRQGASEENGADDTGPRSRLQAAQPPPALTTGLTAAALLLTRILKAQASAR
                     HE"
     gene            933..2135
                     /locus_tag="BHS07_RS40225"
     CDS             933..2135
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BHS07_RS40225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_237079814.1"
                     /transl_table=11
                     /translation="MLKLARKELAVHRRLHGNARAALHRMEARSPELAQARARAYAYAV
                     FPSLGQGSAVLGGTWGLGEVFRQERLVGYAALVQLTVGVQLGGQTSSEVVLFEDQEAFQ
                     RFKHGGTGLALNAAATLVKAGAAKARGPAGSTTVALATDGGMWAGAALGAQRAFVVPAV
                     LTRSSTLRRLLLSIPSLTRGTPAPNEGETRESAMAKPQTSKSGGKRLGKLTAAWKRRKA
                     QAEEKTGPQKVAAHTREAMQRVSTRGRETLEHLRDRVPSGDRLRERAGKARTWAADQLG
                     EHAVAIGLTTLAAGVAGAALLPVSDRERRALSSVSHKVQGLTHSLGATPQVARLTRSVS
                     GAVARVRRGDGAATEEEKPRAPRPKKLAATRGKKAAGKPAAAAKTSRRSRKGGPPPQAT
                     K"
     misc_feature    1455..1457
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2278..3363
                     /locus_tag="BHS07_RS25865"
                     /old_locus_tag="BHS07_25235"
     CDS             2278..3363
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616990.1"
                     /locus_tag="BHS07_RS25865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25235"
                     /product="hypothetical protein"
                     /protein_id="WP_140876395.1"
                     /transl_table=11
                     /translation="MASIRAIALSCIVVLGFVAACGDDSTPTPTDPPDAAEPETCDSVG
                     VALCGDTCTSIETDPAHCGGCDQACGTAEACEGGVCVARCQIGDQQVASGAVNSANSCE
                     QCEPATSATTWTQRTDGTQCNPGQVCSDGVCSPKCFIDGTLYAEGTANPANACETCSPA
                     AATTNWTARASIPLLNGGDDITEQGWTTVAQTPNTLTYGADYVRLSTSTNSDSATSGQL
                     LITRADAFDGTKPFKLQVTMQVESVNDHNVLDSGAAILGSFTRPFGNTVDRSQMIYLDS
                     AAIGWADNTQSAAFSVTDGAYHVYELAVDASKVATVSIDGVAKLTRTHFTTNGTLAIGD
                     QTNDASVNGAVRIKSVALMCP"
     gene            complement(3560..4969)
                     /locus_tag="BHS07_RS25870"
                     /old_locus_tag="BHS07_25240"
     CDS             complement(3560..4969)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554908.1"
                     /locus_tag="BHS07_RS25870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25240"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_140876397.1"
                     /transl_table=11
                     /translation="MSERVIILGAGLAGLAAANALVLAGQDVVVLEARSRVGGRVLTLR
                     QPFLDGMYAEAGAKYVLSGHSTVLGFARRLGLGLDPLPVPHTSALRPFHLRGRRILAPI
                     GDDTGLPHVLRADEQQLGLSGLYRRYVAPLVEAVGDPFHETWPGPGATRLDGLTMREAL
                     AERGASSAAIDAVSLGRFDLVGDGIDASSALGVLRRELLTVGRGGSSAYTVRGGSDRLP
                     AALAETLGGRIQFGWAAKRIEQEQGTVRVYCRGSTGEHRTFSAERLVCAIPFSVLRDIE
                     LAPVFSADKMRAIRELEHTSVVRTFIQYRRRLWAEWGWSSGWLTDLPIMHVLDAAPTQP
                     PPSGILEAYRAGPCARAAGLLSEEERIVMTVRGLSQFAPELSSEVQSGTSYTWDLDPWA
                     RGAFAWFRPTQLTQWGHALATREGRVHFAGDQTSPMPGWMEGALFSGQRAAFEILSGAS
                     GACMAEGLRGS"
     gene            complement(5001..6161)
                     /locus_tag="BHS07_RS40230"
     CDS             complement(5001..6161)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="BHS07_RS40230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_237081442.1"
                     /sec_met_domain="YcaO (E-value: 7.1e-53, bitscore: 179.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGHFLAQVGVGASLEERRLTARAEAIERASSLLRVPDVCATPAR
                     ALAQPFLPMRDWDLYTPEQYASPGFPYAPVTQDTPLDWVRATDAETGEHLLVPAAFTTS
                     ERLVGPRFLCASSNGVATHTSDDAALRSALLEVVERDALQLAWYRGQGARRLDMATLDI
                     DEGVSRHFKGSGWTLHFSYLPGRAGLHVIALIAEATGEGAFPKGGTLLAAAAAGDPAVA
                     VHRALREMRMLTEAVALRRQLAIDFEALRRPPVLENYWLIDSLLDITLLYLNPAMRSAV
                     DLLLEGPPVALPRVRVPDAAGELVTRFRQHGLRTLVINLTLAAASPFRTCQALVLGTQP
                     LAFCPSLLRLGSGLLPRRLPPQNPKPPTSPLKLRRGLLNPYLLPLA"
     misc_feature    complement(5118..5120)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    7274..7288
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.84"
                     /tool="antismash"
     gene            7454..8725
                     /locus_tag="BHS07_RS25880"
                     /old_locus_tag="BHS07_25250"
     CDS             7454..8725
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615348.1"
                     /locus_tag="BHS07_RS25880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="BHS07_25250"
                     /product="lysophospholipase"
                     /protein_id="WP_237081443.1"
                     /transl_table=11
                     /translation="MSRLGLSFIVLFACTSAFASPPPRIETEAESLASTPTPFPVLREF
                     VTEDIYHYRFDIRVGTAPNAIVRVHRVVRERAPWRPRSPAHAIMLLHGDFANFVTNFVP
                     TLGNPASSAPGLAPYLAARDIDVWGVDRRWTLPSANGDISDLGEMGVAQELGDIAIALT
                     FARATRPSGDRTTSRIVLGGFSHGGELAYAYAAANGRHVSAVAVLDAYYDIAPEHAELR
                     ELACANAAAGREALAQGVTDSDNSFFITLGELARSAPQAQSPVFSSYTNRGALLAMNGL
                     TYLLAPYTPLYHLSAPILDANGNVTVLRESSEDSVSAWFASAPPHQSLRETVELDEIWC
                     GTTPRPELSNIRVPLLYLGAAGAFGDYGLYSTTRVSSTDVTALVVRRFGPARVAEDFGH
                     GDLLYAADAPTLAWQPLATWLLSH"
     misc_feature    8936..8954
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.85"
                     /tool="antismash"
     misc_feature    complement(10297..10312)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.74"
                     /tool="antismash"
ORIGIN
        1 tccatcctgg gtgatgcgtg tcgcgctggg ccgcgcgagc gcttcctctc ctttgtcttc
       61 gtcgaggcca tgatgtgcgt cctcctgaca ccgaaggtag acacctccgc gtcacgtccg
      121 ggcaaccaga ccactcacgg cgtggtcgtt cgcacgcacg tcccgtgggt accttgcgga
      181 catggccgtc gccacgttga ttcatcagga ggcgaacatg aagccagagc aggagaaaac
      241 gcttcacgaa gaggtcgtgg caacggtgga ccggctggtg ctcaagaacc cggcgctgga
      301 aaaagagctg gagcaggcac agggctacgc cgtcttcccc tcgctgggca gggcatcgct
      361 ggtcctcggc ggctcgtacg gccacggcga ggtcttcgag aagggcaagc cgattggctt
      421 cgccacgctg agccagctga ccctcggagt ccaggtgggc ggacagaccc tgagcgagct
      481 catcttcttc aaggaccgtg atgccattga agcgttcaag ggagggaagg cggccttcgc
      541 cgccaacgcg tccgccgtaa tcgtgaaggc ggcggcgtcc ggaaccatca actatgcgaa
      601 gtgcgttgca cacgcctact cgcgtggcgg catgctgctc gaggcctccc tgggcggaca
      661 gaagttcacg ttcattccgc gttcgtcgga cgcatcggag cgagggaacg gcggcctggt
      721 gcagcgcttc cgcaggcagg gtgcctccga ggagaacggg gccgatgaca ccgggccgcg
      781 ttcgcggctg caagcggcgc agccccctcc tgccctgacc acggggctga cggctgccgc
      841 cctgctgctc acccggatcc tcaaggcgca ggccagtgct cgccacgaat gaagcgccca
      901 tccgagataa actggtgagc caaggccatc gtgtgttgaa gctcgcgcgg aaggagctgg
      961 ccgtccaccg cagactgcat ggtaacgcac gggcggcatt gcaccggatg gaagcccgct
     1021 caccggagct ggctcaggcg agggcccggg cctacgccta tgccgtcttc ccttcgctag
     1081 gccaggggag cgccgtgctc ggaggcacct gggggctggg cgaggtcttc cgtcaggagc
     1141 ggctggtcgg atacgccgcg ctggtccagc tgaccgtggg ggttcagctg ggcggacaga
     1201 catcttcgga ggtcgtcctc ttcgaggacc aggaggcttt ccaacgcttc aagcatggcg
     1261 ggacgggact ggcactcaac gcggccgcga ccctggtcaa agcaggcgcg gcgaaggcgc
     1321 ggggcccggc tggcagcacg acggtcgcgc tggccacgga tggaggaatg tgggcgggcg
     1381 ccgcgctcgg cgcccagcga gccttcgtcg tgccggccgt cctcacccgc agcagtacat
     1441 tgcgccggct tctcttatcc ataccttctt tgacacgagg aacgccggcc cccaacgagg
     1501 gcgaaacgag ggagagcgcg atggccaagc cgcagacgag taaatccggg ggaaaaaggt
     1561 tgggcaagct caccgcggcc tggaagcgcc gcaaggccca ggccgaagag aagacaggtc
     1621 cccagaaggt ggccgcgcat acacgcgagg ccatgcagcg ggtcagcacc cgagggcgcg
     1681 agacactgga gcatctgcga gaccgggtcc ccagtggaga cagactccgt gagcgagcag
     1741 gcaaggcacg cacctgggca gccgaccagc tgggtgaaca cgcggtggcc atcggattga
     1801 cgacgctggc agcaggcgtc gcgggagcgg cgctgctccc cgtgtcggac cgggagcgcc
     1861 gcgcgttgag ctccgtgtcc cacaaggtgc agggactgac ccactccctt ggagccaccc
     1921 cccaggttgc gcggctgacc cggtccgtga gcggcgcggt ggcacgcgtc cgccgcggtg
     1981 acggcgctgc cacggaggag gagaaacccc gggccccccg ccccaagaag ctcgcggcga
     2041 cgcgtggcaa gaaagccgcg gggaagccag cggcggccgc caagacatcg cgccgttccc
     2101 ggaagggcgg ccctccgccc caggccacta agtagggacg caggccggag cgcccagcgg
     2161 aacgtcgcct gttcccgttg cgcggaaggt gaaatcggac gaggcgttcg tggagggact
     2221 ggaggccagc gcatcgccat tgcgggtgca cagggggcgc gtaaggtggt gggcgaaatg
     2281 gcctccattc gcgctatcgc gctctcctgc atcgtcgtgc tcggcttcgt agctgcttgc
     2341 ggtgacgatt ccacccccac ccccaccgat ccacccgacg cagctgagcc agagacgtgc
     2401 gacagcgtcg gcgtcgcgct gtgcggcgac acctgtacga gcatcgagac cgacccggcg
     2461 cactgcggag gttgcgacca ggcttgcggg actgccgagg cctgtgaggg cggcgtctgc
     2521 gtcgcgcggt gtcagatcgg cgaccagcag gtcgcctccg gagcagtgaa ctcggcgaat
     2581 tcgtgcgaac agtgcgagcc ggcgacgtcc gcgaccacat ggacgcaacg cacggacggg
     2641 acgcaatgca acccgggaca ggtttgctcg gatggcgtgt gcagcccgaa gtgcttcatc
     2701 gacggcacgc tctacgccga gggtacggcg aacccggcca atgcctgcga aacgtgctca
     2761 ccggcggcgg cgacgacgaa ttggacggcg cgcgcaagca ttccgctgct gaacggcggc
     2821 gacgacatca cggagcaagg ctggacgacg gtcgcgcaga cgccgaacac gctcacgtac
     2881 ggcgcggact acgttcgcct ctcgacgtcg acgaacagcg actcggcgac gagtgggcaa
     2941 ctgctcatca cgcgggcaga cgcgttcgac ggaacgaagc cgttcaagct tcaagtgacg
     3001 atgcaggtcg aatcggtgaa cgaccacaac gtgctcgaca gtggagcggc catccttggg
     3061 agcttcacga ggccgtttgg caacaccgtg gatcgttcgc agatgatcta cctcgacagc
     3121 gcggcgattg gctgggcgga taacactcag tccgcggcgt tctcagtgac cgacggcgcg
     3181 tatcatgtct acgagctcgc ggtggatgcg tcgaaggtgg cgactgtcag catcgacggc
     3241 gtcgcgaagc tcacgcggac ccacttcacg acgaacggca cccttgccat cggcgaccag
     3301 acaaacgatg cgagcgtcaa tggcgcggtg cggatcaagt cggtcgcgtt gatgtgtccc
     3361 tgagtatctg cgaaagaatg gcccgacgac gagggggcag gccacgccgc tgccctcagt
     3421 cccgacctgc caaccttcga agcctgcccg acgctgccgg tacctgagtt cgcacggcca
     3481 cctttcgcca catttcaacg gcccccaagg tcgcgaagca ccccccacgt cctctcgcaa
     3541 gtcgcttccg cagcctgaat caggagccac gcaggccttc tgccatgcac gccccgctgg
     3601 ctcctgacag gatttcgaag gcggcgcgtt ggccagagaa gagcgccccc tccatccaac
     3661 cgggcatggg agaagtttgg tcgccagcga agtggacccg gccttcgcgc gttgcgagcg
     3721 cgtgtcccca ctgcgtgagt tgcgtcggcc ggaaccacgc aaaggcgccc cgagcccaag
     3781 ggtccaggtc ccaggtgtag gaggttccgc tctgcacttc ggaggacagc tccggcgcga
     3841 actggctcag gccccgcaca gtcatcacga tgcgctcctc ctcggagaga aggcccgccg
     3901 cgcgggcgca cgggcccgcg cgatacgctt cgaggatgcc tgacggcgga ggttgagtcg
     3961 gtgccgcgtc gagaacgtgc atgatgggga ggtccgtcag ccagccactc gaccaccccc
     4021 actctgccca gaggcggcgc cggtattgga tgaaggtacg cacgacggac gtatgctcga
     4081 gttcgcgaat cgcccgcatc ttgtccgccg agaagacggg ggcgagttcg atgtcgcgaa
     4141 ggaccgagaa cggtatggcg cacacgagcc gctccgccga gaacgttctg tgttctcccg
     4201 tgctgccgcg gcaatacacg cggacggtcc cctgctcttg ttcaatgcgc ttcgctgccc
     4261 atccgaactg aatccttccg ccgagcgttt ccgccagggc ggcgggaagc cggtcacttc
     4321 cgcctctcac ggtgtaggcg ctcgaacctc ctctgccgac agtcagcaac tcccgtcgaa
     4381 gaacgccaag cgccgagctt gcgtcgatgc catcaccaac gaggtcaaac cgccccaggc
     4441 tcaccgcgtc gatggccgct gaagaagcgc cccgttccgc gagggcctca cgcatcgtca
     4501 ggccatcgag gcgggtcgcg cctggaccgg gccaggtctc atggaagggg tcgccgacag
     4561 cctccacgag gggggccacg tacctccgat agaggcctga gaggccgagc tgctgctcat
     4621 ccgccctcag gacatgcggg aggcccgtat catcaccgat gggggccagg atgcgacgtc
     4681 cccgaagatg gaagggccgc aaggccgacg tgtgcggaac tggcagcggg tcgagcccca
     4741 ggccgagcct cctcgcaaag ccgaggaccg tcgagtgccc gctgaggaca tacttcgcgc
     4801 ccgcctcggc atacattccg tccaggaacg gctgccgcag ggtcaggacc cggccaccga
     4861 ctcgagagcg cgcctcgagc acgacgacgt cctggcctgc gaggacaagc gcattcgctg
     4921 ccgccagtcc tgcgaggccc gcgcccagaa tgatgacgcg ttcgctcatt cgagacgtcg
     4981 cctcgcgcct tcggtgagac tcatgccagc ggaagcaggt acggattcag cagtcctcgc
     5041 ctgagcttga gcggcgatgt gggcggcttt ggattctgcg ggggaaggcg gcgcggcagc
     5101 agtccactcc cgaggcgtaa caggctcgga cagaacgcca gaggctgcgt cccaaggacc
     5161 agcgcctggc aagtccggaa cggagaggcc gcagccagtg tgagattgat gaccagggtg
     5221 cgcagcccat gctgtcggaa cctcgtgacg agctcccccg ccgcgtcggg gacccgaaca
     5281 cgagggagcg ccaccggggg accctccagg agcaggtcga cagcgctccg catcgcgggg
     5341 ttcaagtaga gcaacgtgat gtcgaggagg gagtcgatga gccagtagtt ctccaggacc
     5401 gggggccgcc ggagcgcctc gaagtcaatc gcaagctgcc gacgaagcgc cacggcctcc
     5461 gtcaacattc gcatttcacg gagcgctcgg tgcacggcca ctgccgggtc ccccgcggcc
     5521 gcggcggcga ggagcgtccc tcctttgggg aacgcaccct ctcctgtcgc ctcggcgatg
     5581 agtgcgatga cgtgaaggcc ggcgcgaccg ggcaggtacg agaagtggag ggtccagccc
     5641 gaacccttga aatgacgcga caccccctca tcaatgtcga gcgtagccat gtcgaggcgc
     5701 cgcgcccctt gacctcggta ccaggcgagt tggagcgcat cgcgctccac cacctccagg
     5761 agcgcggacc gcaacgcggc gtcatcgctc gtatgcgtcg cgacgccatt ggatgaggca
     5821 cacaggaagc gcgggcccac gagccgttcg gaagtcgtga aggctgcggg aacgagcaaa
     5881 tgctcaccgg tctccgcatc cgtggccctg acccaatcca gtggcgtgtc ctgggtgact
     5941 ggcgcgtagg ggaaccccgg gcttgcgtac tgctccggcg tgtagaggtc ccaatcacgc
     6001 atgggcagga aaggctgcgc gagcgcacgc gccggagtcg cgcagacgtc gggcacacgc
     6061 aggagggacg aggcacgctc gatggcctct gctcgcgcgg tcagcctccg ctcctccagg
     6121 gatgctccga cgcccacctg ggccaggaaa tgtcctgaca ccacgggacg tcctcgtttc
     6181 cgaagacgca gggtggcggc gccccaggtc aaggtgacaa tgggaggagc cccccgcctt
     6241 ccgcggaccg tggcttcgcg cgtcgcgatt cccacgcgac ccagcgcggc accggccatc
     6301 cgcgagccct ccagcttgcc gatgcggttc gccgcgcccc agttcgcgga gcacgcgcat
     6361 cccgccgcgg gccgggaccg gatgcgtctg gcgcctggcc gcccgtaggc gccctctgtc
     6421 caggcacgcg catcgaggga atgcggcttc atggctttcc ggagggccgt cagatcggct
     6481 gcccccagcg caatcacagc gcggagaggg agcgcattcg ccacgacggc tgcctcatcg
     6541 ggcgcatccg cgctctggac ctcccagagc agaccacagg cgacacacac accactgcca
     6601 gcgcttcgcc aacgtgtggc gctcagttgg atgagcgctc cgctgttctc ggaaatgtcg
     6661 gccgtgcgaa gaggccccag tcgcagcctg ccggtgatct ccaatgcctt gcggagggct
     6721 tgctcgtcca tcaaacgtgg agcgcctcgc cccagcgccc cttcctcttc gagccgtgct
     6781 ccgagctccc tggcgccggt agggtcgacg cccgccttca ccagcgcgcg gacgacagcg
     6841 gcgcgccctc cgcggcgaac gtctccgagg atggagacga gaagagcaaa ggggaggccc
     6901 cgtatctcca tcagcgccct gcgggtctga aggatggaga ttcccctcgc catttcgagg
     6961 agcacgacac ctgactgaac tcccatgtcg tgcccgggac gccgctactt cgaaatgtgg
     7021 gaaatgctgg acacggtggt ttcaaatgcc ttgagcgcgg acaccttcag aacgcgcacc
     7081 gtgaccttga gagtctttgc acgcttcgta cgagcaggct tcttcatgtt cggcctccct
     7141 gggtgagcga gaagtgtccc ctcaagccac acttcgagtc aacaacaggc cagggcactt
     7201 tgagccgtga tgggaattgc cgtggcccac acaggagatg ggcctacctg tcagagcgca
     7261 acaccatgcg cgctgacagt ccatttcagt ttcgagctga cctcagacac gccccgaggt
     7321 cagataggac tcacgaccag aggattggca acgacgatat gtccgcgccg gaatcttcgt
     7381 tctcagaggg ccagcccctt cccattctgg gggcgcgtca caacccgtct tgagtgaaac
     7441 tgaagggccc cgcatgtccc gtcttggtct ttcgttcatc gtattgttcg catgcacgtc
     7501 cgcattcgcc tccccacctc cgcggattga aaccgaagcg gaatcgctcg cgtcgactcc
     7561 gacaccgttc ccggtgctgc gtgagttcgt gaccgaggac atctatcact accgattcga
     7621 cattcgtgtc ggcaccgcac ccaacgccat cgtgcgggtc catcgcgtcg tccgagagcg
     7681 cgcgccgtgg cggcctcggt cgcccgccca cgcgatcatg ttgcttcacg gagacttcgc
     7741 caacttcgtc acgaacttcg tgccgacgct cggcaatccc gcgtcatcgg cgccggggct
     7801 cgctccgtac ctggcggccc gggacattga cgtctggggt gttgaccgtc gctggacgct
     7861 gccctccgcc aacggagaca tctccgacct gggcgagatg ggggttgccc aggagctcgg
     7921 agacatcgcc atcgcgctca ccttcgcacg ggcaacgcgg cccagcggcg accgcactac
     7981 gagccggatc gtgctcggcg gattctcgca tggcggcgag ctcgcctacg catatgccgc
     8041 cgccaacggc cgccacgtgt ctgcggtcgc cgtgctcgac gcctattacg acatcgcgcc
     8101 tgagcacgcg gaactgcgcg agcttgcgtg cgccaatgcc gccgcgggac gtgaggcgct
     8161 tgctcaaggc gtgaccgact ccgacaacag cttcttcatc acgttgggcg agctggcccg
     8221 gagcgcgccc caggcgcagt ctcccgtgtt ttcgagctac accaaccgcg gtgcacttct
     8281 cgcgatgaat ggactgacct accttctcgc gccgtacacg ccgctgtacc atttgagtgc
     8341 gcccatcctc gacgccaacg gcaacgtcac ggtattgcgc gagtcgtccg aggacagcgt
     8401 gagcgcgtgg ttcgccagcg cgccgccgca ccagtcgctg cgtgagaccg tggaactcga
     8461 cgaaatctgg tgcgggacga ctccaaggcc cgagctctcg aacatccgcg tcccgttgct
     8521 ctacctcggt gccgctggcg cgttcggtga ctacgggctg tattcgacga cccgggtgtc
     8581 atcgacggat gtcacggcgc tcgtcgtccg ccgcttcggt ccggcgcgag tggccgagga
     8641 cttcggccat ggcgacctct tgtacgcggc agatgcaccg acgctcgcgt ggcaaccgct
     8701 cgcgacgtgg ttgctgagcc actgaggaga agcatctccc gggagactgg ggtggcctac
     8761 ccggccgcca ccttcgtggc ctggaagaag tccgccggca tcggccgctc cagcgcccaa
     8821 aggatgcgca tcggccgctc cgactcgtga ctcacgtagc ggaccggccc caggcacagg
     8881 tagggcattg gctcgccacg gtcgtccctc ttgcgctcgc gcacgaagag gatgacatgc
     8941 gaccctctct gaacgtgctc tacgtaccgc cggcccgtgg gcgacccgga cgacgtggcg
     9001 ttctgcgact cccaatgaaa cagggtgggc gagacggcat agtccgcata gcgggtggtg
     9061 ggcttgaagt ccgcatccga cttgtcgagc gtgacgaaca gcaggtccgt tcgatgcgct
     9121 tccacccaca ggacgccttc tcgcacttcc cgcagggtcc ccttcgttcc cagaacgccg
     9181 taggcggcaa tgacctcgta gagaccatag gtcgcatggc tcgcgagggg cacagcccca
     9241 gaagggtcca tcggcgtatg aaccctgcgg ctccggtctg cgagcaactc cagcagctcc
     9301 agcaactcct cgcgaagggc ggcgcaggac cacagctcgg acatcgcttg aggccactcc
     9361 accaccgcgc ggctccggaa gcccagcgcg gaaaagaaca tccactggag gcgctgctcc
     9421 cggctgcctg gcgccgccgg ctccggcggt gcggccaagg acacccaggt atgccactgc
     9481 ctcaaccgtt cttcgtcccc cacgtgaagc atgcgcgcca aggccttgga cagcgcggcc
     9541 tcgtacggcc cggcagggcg gttctcataa ccggcgtcgc ggcgcagcgt cgtgaaggcg
     9601 ctgcgctccg tgggccgtcc gtagacgtcc tccaactcgt agccagtggc gcccaggaaa
     9661 gccgacaggc gcgtctcggg aggcagtgac tggagatcct cgagcaacat gcgccgtgcc
     9721 tggaggacgg cagcgcggac gttttgtagg acatgctccc gagcaatctt gtccaaccga
     9781 atggcacagc caggaggcag gcgtgggaag tcgtgctcca gctcggtctc taactgccga
     9841 cgcgtcccgc cgacgatagc ccgatagcgc acgtcgaagc ggtagcgctt gtgcgcctgc
     9901 ccgatgaagt cgagcaccgt gaggacgctc ttgccggaac catgagacca acgcagcccg
     9961 cggcccaatt gctggaggaa gatggtggcg ctctccgtgg gtcggagaag aaggagtgtg
    10021 tccacctcag ggatgtccac gccctcattg aagaggtcca cggtaaagac ggcttgaagc
    10081 tcgccgcgcc ggagccgagc aacagcatct cggcgctctc cgtccggcga accggcatgg
    10141 agggccacgg cattgagtcc cgcggcgttg aagtgctggg ccatgacctc cgcgtgtgtg
    10201 acaccgacgc agaatcccag tgcgcgcatg gcgtgcggga tgtgaacgta ctccgcgacg
    10261 gcctgaagga tgcgccgggc ccggaggtca tcgccggtgt acaaagcgtc aagctcgccc
    10321 ctcgcgtaca tgccccgctt gaacgtcacc gatgagaggt cggtgccgtc atgcacccca
    10381 aaatattgga aggggcagag caggccctgt tcgagggcct gccagaggcg gacgtcgcag
    10441 gcgatgcggc cgtcgaacca gcccagcacg gactgtccgt ctcctcggtc cggagtcgcg
    10501 gtgaggccga gcagaatgcg cggcttcaag cgctccagca gtttgcgata cgtgggcgcc
    10561 tcggcatggt ggaactcgtc caccaccacg acgtcgtacg cgtccggtgc cagcgtttcg
    10621 agctgccgct gtagggactg cacggaggcg aagacgtggc gggccccgta gagtggccgc
    10681 tcgccagcga cgagccgctc gccgaaggcg gagtcccgca acaccaactg aaagacctgc
    10741 tggctctgct ggaggatttc atcccggtgc gcgacaaaca ggagccgctc atgtccctgg
    10801 gcacgcagcc gccgatagtc gaaggcagcc acccatgtct taccggtgcc tgtcgcggca
    10861 acgacaaggt tcttccgatg cccatgttgg cgctccgcct ccagcgcttc gagcatctgt
    10921 cgctggtggg gcttcggctc gaactcgatg ttgagcgcca caagacgctc caacgaggac
    10981 gcggaggaac cgccccgctc ccgggcgagt gccgtggaca gccgctcacc atcgcggtct
    11041 gggtcgtagg agacgaactc cggttcactc cagtactgct cgaatgttgc gccgacccgc
    11101 tcgaggatgc cggcgttgtc cgactggctc acgcgcacgt tccactccag gccgtccacc
    11161 a
//
