LOCUS       NZ_VFSH01000010        12154 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus shenyangsis strain D19 Scaffold10, whole genome
            shotgun sequence.
ACCESSION   NZ_VFSH01000010
VERSION     NZ_VFSH01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus shenyangsis
  ORGANISM  Streptococcus shenyangsis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 72214)
  AUTHORS   Xiao,C., Li,X., Sun,Y. and Liu,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2019) Key Laboratory of Environmental Pollution
            and Microecology of Liaoning Province, Shenyang Medical College, No.
            146, Huanghe North Street, Shenyang, Liaoning 110034, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAP denovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006385785.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 12:53:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,899
            Genes (coding)                    :: 1,820
            CDSs (with protein)               :: 1,820
            Genes (RNA)                       :: 55
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 23 of 79
            Pseudo Genes (incomplete)         :: 50 of 79
            Pseudo Genes (internal stop)      :: 25 of 79
            Pseudo Genes (multiple problems)  :: 17 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:16:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 30543
            Orig. end                         :: 42697
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VFSH01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12154
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12154
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12154
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7154](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7154)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    2593..2611
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.95"
                     /tool="antismash"
     misc_feature    2679..2692
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.09"
                     /tool="antismash"
     gene            complement(2762..3469)
                     /gene="purC"
                     /locus_tag="FJR77_RS07730"
                     /old_locus_tag="FJR77_07730"
     CDS             complement(2762..3469)
                     /EC_number="6.3.2.6"
                     /GO_function="GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="purC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /locus_tag="FJR77_RS07730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07730"
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="WP_140834208.1"
                     /transl_table=11
                     /translation="MSKQFIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAGK
                     GVLNNQISSFIFEKLNAVGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSKRF
                     GVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINELLKA
                     WFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLGELTD
                     VYEIVWEKLQELK"
     gene            complement(3639..4988)
                     /gene="comB"
                     /locus_tag="FJR77_RS07735"
                     /old_locus_tag="FJR77_07735"
     CDS             complement(3639..4988)
                     /codon_start=1
                     /gene="comB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /locus_tag="FJR77_RS07735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07735"
                     /product="competence pheromone export protein ComB"
                     /protein_id="WP_140834206.1"
                     /transl_table=11
                     /translation="MKPEFLESAEFYNRRYHNFSSRVIVPMSLLLVFLLGFATLAEKEM
                     SLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESYVS
                     QLDMLKDQKKQLEYLQKSLQEGTDYFPEEDKFGYQATFRDYLSQAGSLRASTSQQNETI
                     ASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIEIGASLASQNLAYSLYQSYKSQGE
                     ENPQAKAQAVAQVEAQLSQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKV
                     GQELTLLDQKILEVESGKKVQGNLLDKGKITASEDGVLHLNPETSDSTMVAEGALLAQL
                     YPSLEKEGKAKLTAYLSSKDVGRIKVGDSVRYTTTHDAKNQLFLDSTITSIDSTATKTE
                     KGNFFKIEAETNLTSEQVAKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            complement(5001..7154)
                     /gene="comA"
                     /locus_tag="FJR77_RS07740"
                     /old_locus_tag="FJR77_07740"
     CDS             complement(5001..7154)
                     /codon_start=1
                     /gene="comA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /locus_tag="FJR77_RS07740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07740"
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="WP_140834205.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 7.7e-44, bitscore:
                     141.1, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1127.3,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYSLAHLRELAKTT
                     MDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTGQD
                     KDSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKDQKNGLLSFIPILVK
                     QRGLIANIVLATLLVTLINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYILQQIL
                     SYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSIIDALAS
                     TILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNRDTMEAN
                     AVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFIYSRAESQQKALKKVAH
                     LLLNVGILWMGAVLVMDGKISLGQLITYNTLLVYFTNPLENIINLQTKLQTAQVANNRL
                     NEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTIPQGSKVAFVG
                     ISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQPYVFNGTILE
                     NLLLGAKEGTTQEDILRAVELTEIREDIERMPLNYQTELTSDGAGISGGQRQRIALARA
                     LLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAERTEKVVVLDQGK
                     IVEEGKHADLLAQGGFYAHLVNS"
     misc_feature    7283..7296
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.97"
                     /tool="antismash"
     gene            complement(7574..7777)
                     /locus_tag="FJR77_RS07745"
                     /old_locus_tag="FJR77_07745"
     CDS             complement(7574..7777)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869798.1"
                     /locus_tag="FJR77_RS07745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07745"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_140834204.1"
                     /transl_table=11
                     /translation="MKFVKSILKVWPEIMVLLSSMSYIIIRLVADMNKVSLPTWLDSFN
                     IPTIALFVMVFILLYRSKEKKE"
     gene            complement(7856..8035)
                     /locus_tag="FJR77_RS07750"
                     /old_locus_tag="FJR77_07750"
     CDS             complement(7856..8035)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846993.1"
                     /locus_tag="FJR77_RS07750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07750"
                     /product="hypothetical protein"
                     /protein_id="WP_033679470.1"
                     /transl_table=11
                     /translation="MKTFLLGFAEGYFTVSLIIYIATYLILKNPINTLFYPEIYPVLFG
                     LFYLGYKVNKKRVE"
     gene            complement(8116..8331)
                     /locus_tag="FJR77_RS10360"
                     /old_locus_tag="FJR77_07755"
     CDS             complement(8116..8331)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755479.1"
                     /locus_tag="FJR77_RS10360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07755"
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_078353051.1"
                     /transl_table=11
                     /translation="MNNRDLNLNTLLPKLATIFPLLGITLNVALHVIRTGSLVSFNWQW
                     TLVGLLFSAYFGIFRKDLSKNNQNYK"
     gene            complement(8335..8502)
                     /locus_tag="FJR77_RS07760"
                     /old_locus_tag="FJR77_07760"
     CDS             complement(8335..8502)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284951.1"
                     /locus_tag="FJR77_RS07760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07760"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_078353132.1"
                     /transl_table=11
                     /translation="MNLEQFYQLSDDELVGTKGGFGLAEAVGIAGLLNAAVQIFNAGYK
                     FGSDFARRGR"
     gene            complement(8511..8711)
                     /locus_tag="FJR77_RS07765"
                     /old_locus_tag="FJR77_07765"
     CDS             complement(8511..8711)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478863.1"
                     /locus_tag="FJR77_RS07765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07765"
                     /product="hypothetical protein"
                     /protein_id="WP_023940558.1"
                     /transl_table=11
                     /translation="MFLKKEVILLGVLLSLRIIFTIVQILIDYKILSGQYSVNFMDISE
                     VFTYPILILIIYIAIKYNTKE"
     misc_feature    complement(8697..8710)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.08"
                     /tool="antismash"
     misc_feature    8752..8763
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.9"
                     /tool="antismash"
     misc_feature    8778..8792
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.73"
                     /tool="antismash"
     gene            complement(8802..9002)
                     /locus_tag="FJR77_RS07770"
                     /old_locus_tag="FJR77_07770"
     CDS             complement(8802..9002)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055966.1"
                     /locus_tag="FJR77_RS07770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07770"
                     /product="SPPN_00645 family bacteriocin-like peptide"
                     /protein_id="WP_049512841.1"
                     /transl_table=11
                     /translation="MNMIKIEEKFTSLTEKDLATVEGGRVSWRGNINIDVHKVIDSTGR
                     FFGGIYNAGRAFGRNVYNAFY"
     misc_feature    9027..9040
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.44"
                     /tool="antismash"
     misc_feature    complement(9028..9041)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.58"
                     /tool="antismash"
     gene            complement(9035..9223)
                     /locus_tag="FJR77_RS07775"
                     /old_locus_tag="FJR77_07775"
     CDS             complement(9035..9223)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439599.1"
                     /locus_tag="FJR77_RS07775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07775"
                     /product="hypothetical protein"
                     /protein_id="WP_140834203.1"
                     /transl_table=11
                     /translation="MERIIESWITRVENFYSLQSIPVKVLIFIVGVVGVYALGTLLLGP
                     IYWAGQKFGEMVYNYLF"
     gene            complement(9243..9425)
                     /locus_tag="FJR77_RS07780"
                     /old_locus_tag="FJR77_07780"
     CDS             complement(9243..9425)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435077.1"
                     /locus_tag="FJR77_RS07780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07780"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_140834202.1"
                     /transl_table=11
                     /translation="MEQIKNMENSFHVMSHEELADVEGGLLLGLVVVGGVAIAGTFLLG
                     AVDGASGAHAKRRGR"
     gene            complement(9535..9675)
                     /locus_tag="FJR77_RS07785"
                     /old_locus_tag="FJR77_07785"
     CDS             complement(9535..9675)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004581.1"
                     /locus_tag="FJR77_RS07785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07785"
                     /product="hypothetical protein"
                     /protein_id="WP_181146624.1"
                     /transl_table=11
                     /translation="MNEMNDKYTIEVLKESDLALLVGGSKESYQRGLMVGLILRRALFG
                     F"
     gene            complement(9687..9815)
                     /locus_tag="FJR77_RS07790"
                     /old_locus_tag="FJR77_07790"
     CDS             complement(9687..9815)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658145.1"
                     /locus_tag="FJR77_RS07790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07790"
                     /product="smi_0059.1 family bacteriocin-like peptide"
                     /protein_id="WP_000658221.1"
                     /transl_table=11
                     /translation="MKEHYYGFVELTSEELTDIQGGENWTKTLVDWYIGFRRGFGF"
     misc_feature    complement(9890..9903)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.28"
                     /tool="antismash"
     misc_feature    complement(10014..10027)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.13"
                     /tool="antismash"
     gene            complement(10185..10916)
                     /locus_tag="FJR77_RS07795"
                     /old_locus_tag="FJR77_07795"
     CDS             complement(10185..10916)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221865.1"
                     /locus_tag="FJR77_RS07795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_07795"
                     /product="response regulator transcription factor"
                     /protein_id="WP_140834201.1"
                     /transl_table=11
                     /translation="MRILVLEDDKVQQGRIEQTLLDIGRSRNLRLEIDIAKNYGDVEKY
                     SQYFDHYQLYLLDLEIDGERERGFQVAQQVRERDPFTSIVFVSTHSEALPLAFRYHLSA
                     LDFISKDQPEADYRHQLERCLDYVLAVDKRENMRLFTYSFEGRRGFTLPYHEILAFETS
                     VESHRIKVYYANHSIKTIYGSLKDIAAKAEKDYFVYANRNTLVSLQAIEEMTATEVTLL
                     EGLHFPVSRTARRTLKKHLNV"
     gene            complement(10913..11728)
                     /locus_tag="FJR77_RS10365"
     CDS             complement(10913..11728)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.5"
                     /locus_tag="FJR77_RS10365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_235888725.1"
                     /transl_table=11
                     /translation="MTNINSSFYVRFDTTISLLIFILFFSWLFYIKHLEQVYRDEQTIQ
                     RQEDENRSLQGMVDKLGHLYDEVRGFRHDFAGIVASMEPAIANQDMTEVSTIYQDVFLK
                     TNEKLRKADYTAFNLHNIHDIAIRNTLAKAMIVADKQGIHFSLETVGVVEELALPMLEA
                     IRILSILTTNALEAASEAENPQIRVALLAGDRSVRFIIENTRKKEELNPSILSQKGYST
                     KGNHSGLGLATLEDMVFHYDLNLDTQLGETTFRQDLELPFKDEKRGGEE"
ORIGIN
        1 tgtaaagaca aatccaccca attgagctgt caaagccgtt tcaagttcag gcaagcttac
       61 ttctgcaccg atatgatttc caaaagtagc aagtgccaaa ctttcaacca caccaccata
      121 tttgacagct gatgcagatg tcactttgtg accagcttgg atgtcttcaa actgagcaaa
      181 attagactta atcaagtcaa aatcaatctc tgtagagagg gcttgacctg ggatgtagta
      241 gatattttct ccagcagctt taaattctgg agagagcaca ttacggctat ctgccgtcgt
      301 caccccaaag gcaaccaagg ttggcggaac ggtcaattct tcaaaagtac cagacataga
      361 gtccttacca ccgatagatg gcaaaccaag ttgaatctgt gcttcgatag agcctagaag
      421 agctgctact ggctggccaa aacgctcggc ttgtttgtcc atccgctcga aatactcttg
      481 gtaagagaaa cgagccttag accagtttgc gccagcagcc accaaacgag cagttgcttc
      541 gataaccgca taagcagcac cgtggtatgg agaccattca gctacatatg ggttgaaacc
      601 ttgagccatg actgaggcag tatgagtcac accgtgttgt acaggcaatt tctgcacaga
      661 agcctcagtc ggtgtgagtt ggtagcgacc accaagcgga tggttgactg tagaacgacc
      721 gactgagcaa tcaaagatag tctgcaagcc tttttgactc gcatggttca agtcagatag
      781 aaccgtatgt gtatcaaatt caagtgtgtc agcgcttgtt gtgcgctctt ctgggagttt
      841 gacatccttg tccaccacct ttgcatcaac gaccacgcgc acaccattgg tatcaaggaa
      901 acgacgttcc aagtcgacaa tggtttcacc attccagtgc ataaccagat ttggtttttc
      961 agtcaccgta gccaccacaa cagcatcaat attttctttg ttacattcta caacaaaatc
     1021 atctacgtcc tcaggacgaa ccacaactgc catccgttct tgtgattcag agatagcaat
     1081 ttcggtacca ttcaagcctt ggtatttaag aggaaccttg ttgaggtcga tttcaagacc
     1141 gtctgccaat tcaccgatag ctacacagac accaccagca ccaaagtcat tggacttctt
     1201 gataagacga gtcacatcgc cattacggaa cagacgttga atcttgcgtt cttcgatggc
     1261 attccctttt tgaacctcag caccagcagt ctctacagac tcaactgttt gaactttaga
     1321 agagcctgtc gcaccaccga caccatcacg tccagtctta cctccaagaa ggataatgac
     1381 atcacccgct tctggttttt ctcggacgac attgcccttg ggagcagcac ctacaactgc
     1441 acctaactcc atacgtttag caacaaagcc tgggtggaag tactcacgaa cgtaggtcgt
     1501 cgcaagccca atctggtttc cgtaagaaga ataaccatga gcagctgtct tagaaatgac
     1561 ctgttgtggc aatttcccag cacgcgtttc cgaaatcggt gctgtaatat caccggcacc
     1621 tgagatacgc atagcttggt aaacgtatga acgtcctgat aatgggtcac gaatggctcc
     1681 accaatacag gtagcagctc caccaaatgg ctcaatctca gttgggtgat tgtgggtttc
     1741 gtttttaaac atgaggagcc atggttcttt cacaccatta acgtccactt cgatttctac
     1801 tgagcaagcg ttaatctcgt ctgacacttc catatcgtcc aaccgcccat tagcacgctc
     1861 atatcgaccg aaaatagtcg ccatatccat caaggtttgc ggtttttccg aacgccccaa
     1921 ctcatcacgc atggcaatat acttgtcata ggttgactgc aattgctttt ggaatttaga
     1981 agctgaaaag tcgatgtgct tcaactcagt ctcaaaagtc gtgtgacggc agtggtcaga
     2041 ccagtaagtg tccaaaacct tgagttccgt ctcagttggc acacgtccaa ttgacttgaa
     2101 ataatcttgg ataaagagca aatcatccac ttccatagcc atcccttgct cgtccttgta
     2161 gcgagcaaag tcctctgctg tatagctttc aaagaaagtc aatttcggaa tagtcttgtc
     2221 tgactcagaa aattcctgct tggaaatccc tgtcgtgatg tccttgaaac gagaatcaac
     2281 tggattgagc aagtagtttt tgactgcttc taattcggtc gaatcaatat ctttattgac
     2341 caagtaaagt tgggctgtat tgactattac atcactcgaa cttcctagca aaagcaaggc
     2401 ttcctgtgaa gaagctgcac gctggtcaaa ttgacctggc aggctttcaa tggcaaagaa
     2461 agcatagtta gccagatccg cctgtacagc agcttcgtcc aagacatggt ctgtcacctg
     2521 ctcagagaaa atgtgtttct ctgcaggcgc aaacaagtcc tctgccaaat caaatacatc
     2581 atacacttgc acgatgcgaa tacttttcaa acttgacagt cccaagttat gcttgagttc
     2641 tctaaccaaa ctctctgact tgacctgaaa atcagccttt ttttcaacaa aaatacgttt
     2701 atccatcaat ctttattcct ttatttcagc tcttgcaata tccccaacga ccttgaggtt
     2761 gttatttcaa ttcctgcaac ttttcccaaa caatctcgta aacgtctgtt agttctccca
     2821 atcctctacg gaaaacatcc ttatccatgt ggttgccatc cgcatcccac aaacggcagt
     2881 tatctggtga aaattcgtct gccaagataa tcttgccatc cttatcaaaa ccgaactcta
     2941 gcttaaagtc aatcaactta agcccaatct cagcaaacca agctttcaag agttcattga
     3001 tacgacgcgt ttcttccttc aagtaggcaa tctgctggtc atccgcaatc tgtaggaatt
     3061 tcacatgctc gtcattgatg aatggatcat ccaaatcatc atttttatag taaaattcga
     3121 caatcggagt ctccaaggcg attccctcat ccacaccaaa acgttttgaa aaggaaccag
     3181 ctgtatagtt gcgaagcacg acctccagag gaataatctt aaccttttta ttgagttgtt
     3241 ctgtatctga aagtttctcc acaaagtgag tcgccacacc aaccgcattt aatttctcaa
     3301 aaataaaaga tgaaatctga ttattcaaga ctcccttacc tgcaatctgc tccttcttaa
     3361 caccattgaa agcagtcgcc tgatccttgt aagttgaaat aataagattt tcatcctcag
     3421 ttgtatagat atctttagct tttcccgaat agataaattg ttttgacatt taaaagttcg
     3481 cctttcgtat tacttgagca cattctacca taaaaaactt aaaattacaa gaatttaacc
     3541 gaataaataa cgtatcgaca ttaaaaatcg taaagaaaaa actgcaagaa gaatctttct
     3601 cacagtttta aaatcattta actctaaaaa cacgaacatt actctttgtt caaaaattga
     3661 tccaaataat aacgtagata acttttctta cccgtaatca tctgcaggcg accttccacc
     3721 ccgtaccgaa gttttgcaac ctgctcagaa gttagattag tctccgcctc aattttaaag
     3781 aaattccctt tctcagtctt agtagctgtc gaatcaatgc ttgtaatagt agaatctagg
     3841 aaaagttgat tcttggcatc atgagtcgtc gtatagcgaa cagaatcacc gaccttgatt
     3901 cttcctacat cctttgaact aagataagct gttagtttgg ctttcccttc tttttccaag
     3961 gacggataaa gttgggctag tagggcacct tctgcaacca tggtagaatc actggtctca
     4021 ggattaaggt gaagcactcc atcctcactt gccgtaattt tccccttgtc taaaagattt
     4081 ccctgtacct tcttacctga ctccacctcc aagattttct ggtctagaag ggtcaattcc
     4141 tgaccaacct tcgctaagtg ttgggattta agggactcca attgactgct taagcctgac
     4201 gcataggctt gctgggtacc tgaacctgca tactggacac ggtaagtagc aagactggat
     4261 tctaactgag aaagctgcgc ttcaacctgc gcaactgcct gagctttagc ttgcggattt
     4321 tcctcaccct gagacttgta ggactggtag agggagtagg ctagattctg actggccaag
     4381 gaagcgccta tttcaatagc tgacttagct gtctggtaat cgcgaatttt agcctctgtt
     4441 tggctgatga gattgccgat ttcggcttgg gtttggctag ctgctgcatt ctgggacgcg
     4501 atggtctcat tttgttgcga tgtactagcc ctaagactac ctgcttggct gaggtagtcg
     4561 cgaaaggtgg cttggtagcc aaacttatcc tcctctggaa agtaatccgt cccttcttgc
     4621 aggctctttt gcaaatactc caattgcttt ttttgatcct taagcatgtc caactgactg
     4681 acataagact ccgcttggac accctctgcc ccttcctgat attgaaccag aagatctccc
     4741 ttcttaacca gcttattttc ttccaaatga ttgacaagaa tgcgattgtt gctagttgac
     4801 tggatatttg caaggattcg actaggctca acagtagctc tagtagacaa gctcatctcc
     4861 ttctctgcaa gagttgcaaa gccaagtaaa aacacaagta gaagggacat gggtacaata
     4921 acccgactgg aaaaattatg gtaacgacga ttataaaact ccgcactttc taaaaattct
     4981 ggtttcatcc tctcctcttt ctagctattg accaaatggg cataaaatcc accctgtgca
     5041 agcaaatcag catgctttcc ttcttcaaca atcttgccct gatccaaaac aacaaccttc
     5101 tctgtccgct cagcaatggt caagcggtga gcaatgaaaa tcaaggtctt gtctaaagcc
     5161 ataagattat cgacaattcg cttctctgtc agaatatcca aactgctagt tgcctcatcc
     5221 aaaatcaaga ccggcgcatc tgtcaagaga gcacgcgcta gagcaattct ctgacgttga
     5281 ccacctgaga ttcctgcccc atccgaagtc aattctgtct ggtaattcag gggcatgcgc
     5341 tcaatgtcct cccgaatctc tgtcaactcg accgcccgca agatgtcctc ctgagtcgtc
     5401 ccctccttgg ctcccaaaag aagattctcc aaaatcgttc cgttaaagac atagggctgt
     5461 tgaggcagat agttgatata ctggcgcagg gcctttttat caatctgatt gagattgaca
     5521 ccacccagac taatctctcc ctgacttggg tcgtaaaaat tcaccatcat cttagccaag
     5581 gtcgtcttac ctgaccctga aatccccaca aaagccacct tagagccttg gggaatggtc
     5641 aaattgatat ctgacaagac atctcgacca tagccatact tgtaatgaac ctgcttgaag
     5701 gtcatatctc ccttcatcaa gctcaaatcc tcaaccgttt tcttctcctc aaactccgaa
     5761 gctactagat aaacttcatt cagacggtta ttggcaacct gcgctgtctg aagcttggtt
     5821 tgcagattga tgatattttc caaaggatta gtaaagtaaa ccagcaaggt attataggta
     5881 atcaactgcc ccaaacttat cttgccatcc atgaccagaa cagcccccat ccagagaata
     5941 ccgacattga gcaagagatg ggcaactttt ttcagagcct tttgctgact ctctgcccga
     6001 ctatagataa aggatttttt cagataatcc acaaattcct tgtcaatctt ttggtaacgc
     6061 tgactttcac tggtcaaaga cttaatggtc tcaataccgt tgatgtcctc gatgatagaa
     6121 gaagacagaa ccgcattggc ttccatggta tcccgattca tcttctcaaa cggcttcata
     6181 aaagcaaaga taattactgt gtaaatagga agcgccaata aggtcatgaa aaagagattg
     6241 gtattttgtg aaaataaaac aagtgaaata atgacaaccg ttgacacatc taggaaaatc
     6301 gaaagaatgg tcgaagccag cgcatcaatg atactgttag catctgtaaa acgagacacg
     6361 atttcccctg tccgtcgtgt cgcaaaaaag gacataggca ggtgaaaaac atgtttgata
     6421 taggataaaa tcacgtcaat cgacaagcgt tgccccaaaa caagtaagag atactcctga
     6481 gcgtaagaca agatttgctg gagaatataa acaatcacca gcccaataga aataatcccc
     6541 agtgtcgaac gcatctgatc tggcacatag gtatcaatga tagactgcag ataataagaa
     6601 cccacaatgt taatcaaggt taccaagagt gttgccaaaa cgatattagc aatcaagcca
     6661 cgctgcttca ctaatatagg gataaaagaa agcagaccat tcttttgatc cttatgaggc
     6721 ttgtagtctg gactaggtgc cataaaaaga gtcacccctg tccattcttc cgcaaaacgc
     6781 tcacgtggca gtttagttaa cttcaccccg ggatctggat cggcaatatg aatgctatcc
     6841 ttatcctgcc cagtcaccac atagtagtgg agcaatttcc cttccttaag cacatgagca
     6901 acaaaaggaa aagtcaaatc tggcaaatca aagagcgtca tatccgcctt gatggctcgt
     6961 gtctcaaagc caatctcctc tgccaccttg accaagccca aagccgtcgt cccatccatg
     7021 gtcgtcttgg ccaattctcg caagtgagcc aaagaataat agctaccata gtagccaaaa
     7081 accatagcta atgaagctac accgcagtcc atctgatcca cctgcggacg atagtgacgt
     7141 ttcccaaatt tcatattcat ctccttttcc taaactcatc tgataacaac tatcttaccc
     7201 caatccccaa acaaaaactg gacttccttc ccaaatgcgc ctatctccct cccaactgca
     7261 cttttcggat aaaaaaatag gcagaacaaa cgtcctacct actaaaaata tggtatgaaa
     7321 ggaagttgat tttttactgt caccctaggc aagcttggga caaataaatc attgatagta
     7381 acctttacga tatcctattt aaagttaatt caatttttaa tgtccctgct atttctaagc
     7441 cacctctggt ataatctatt catagtcaga atagataaca ccgttctaaa ttacgattca
     7501 tagttactcc gttttttgat tcgttactat aagacaccta ttccctatag agcgtctact
     7561 gcttcaaatt tatctattct tttttttctt tacttctata taaaagaatg aataccatca
     7621 caaataatgc aatcgttggt atattgaaac tatcaagcca agtaggtaaa gatactttat
     7681 tcatatcagc tactaatctg atgatgatat aagacattga actaagtaat accataattt
     7741 caggccaaac tttcagtatt gatttaacga atttcatatt aaactccgat gtgatgcatt
     7801 cttagattct agcctagcta ttttcagcgc agactaacaa gtcattctta actgtttact
     7861 ctactcgctt tttattgacc ttatatccta gataaaagag cccaaataaa acagggtaaa
     7921 tttctggata aaatagggta ttgatcggat ttttcaaaat caaatacgtt gcgatgtaga
     7981 taatgagtga tacggtaaaa taaccctcag caaaacctaa taaaaatgtt ttcataagaa
     8041 actcctaatt acctgacaag agtggcggaa aaacgctacc attcatatac acaaaatcct
     8101 tttgattcat gttcttcatt tatagttttg attatttttt gacaaatctt tgcgaaaaat
     8161 accaaaatat gctgagaaaa gcaaaccaac caaagtccat tgccaattaa aggatactaa
     8221 cgaacctgta cgaatcacat gaagcgcaac attaagagtt attcccaaca aaggaaatat
     8281 cgtagctagt ttaggtaaca atgtattgag attaaggtcg cgattattca ttatctaacg
     8341 cccacgacga gcaaaatctg aaccaaattt gtaaccagca ttaaaaattt gtacagcagc
     8401 attcagtagt cctgcaattc caactgcttc cgcaagacca aaaccacctt ttgttcccac
     8461 taattcatcg tcagataatt gataaaattg ttctaagttc atattcgtca ttactccttt
     8521 gtattatatt ttattgctat gtaaatgatt aaaatgagta ttggataagt aaatacttct
     8581 gaaatatcca taaaatttac actatactgt ccagataata ttttataatc aattaaaatt
     8641 tgaacgatag taaaaataat acgtagcgat aacaataccc ctaataatat cacttctttt
     8701 tttaaaaaca tagaaatacc tctctgacta caatcagatt atggtctatt cgaaaacgaa
     8761 ttcaaaaata taatatatga tattcatttt aataaactat attaataaaa tgcattgtaa
     8821 acatttcgcc caaatgctct accagcatta tagataccac cgaaaaatct tcccgtacta
     8881 tcaatcactt tatggacatc gatgttaata ttacctctcc atgacactcg accgccctct
     8941 actgttgcta aatctttttc tgtaagactt gtaaattttt cttcaatttt tatcatattc
     9001 attatgtctg ttcctcttta tctataattt tttattaaaa taaataattg taaaccattt
     9061 cgccaaactt ctgccccgcc caataaatcg gtcctaaaag taatgtccct aaagcataaa
     9121 ccccaactac tccaactatg aaaattaata cttttacagg aattgactgc aaactgtaaa
     9181 aattctctac cctagtaatc caactttcta ttatgcgttc cattagcgct cttccttccc
     9241 tgttaacgtc cacgacgctt agcatgtgct cctgaagcac catctacagc acccaataaa
     9301 aatgttcctg caattgcgac tcctccaact actactaaac caagcaacaa gcctccttct
     9361 acatcggcca attcctcatg actcattaca tgaaaactat tttccatatt tttaatttgt
     9421 tccatcttat tctccttttt tgattattaa cgattgaaaa ggtagttatt tatctttcct
     9481 ttttcatata ttctgttcat tgttgtaggt aaactttact ctctaaatct tatcttaaaa
     9541 tccaaataac gcacgacgta aaattaaccc caccattaaa ccacgttgat aactttcctt
     9601 gctaccaccc accagcaagg ctaagtcgct ttcctttaat acttcaatag tatacttatc
     9661 attcatttca ttcatatctt atcctcctaa aaaccaaaac ctctacgaaa tccaatgtac
     9721 caatctacaa gggtcttcgt ccaattttct ccaccttgaa tatcggtcaa ttcttccgac
     9781 gttagctcaa caaatccata gtaatgttct ttcattattt tccttccttt cttttttctt
     9841 ctcacttatt tattcgaaaa aattttctaa aatgtacaac taaattaaat ttatttaaaa
     9901 aataactcag tgaaccatgg gtagaacatt gatataaaca taaaccagac tcctttcctt
     9961 ttcctcttat ttatctattc gaaaaacaat tcaaaaatgt acagctaatt aaatttattt
    10021 gaaaaataat ttagtaaacc atggatagaa aattggtaga tacatgaact aggctccttt
    10081 ctgtttcctc ttacttatct attcgagaaa gaatctaaaa aaataagaat atcagaaatt
    10141 tttccgacat tcttattgat aaactcatcc aagtctaaca gatattagac attaagatgt
    10201 tttttaagtg ttcttctggc tgttcgtgat acggggaagt gcaagccttc taacagggtc
    10261 acttctgtcg cagtcatctc ttcaatggcc tgtaaactaa ccaacgtatt gcgattggca
    10321 tagacaaagt aatctttttc agccttggca gcaatatcct tgagactgcc ataaatggtc
    10381 ttgatagaat gattggcata atagaccttg atacgatgag attccactga ggtttcaaag
    10441 gctaaaatct catggtaggg tagagtaaag ccccgtctcc cctcaaaact ataggtaaag
    10501 agacgcatat tttccctctt gtccactgcc agtacatagt ccagacagcg ctccagctga
    10561 tgacggtagt ccgcctctgg ctgatccttg gaaataaaat ccaaggcaga caagtggtag
    10621 cgaaaagcta gtggcaaagc ctccgagtgt gtggacacaa agacaatact tgtaaaagga
    10681 tcccgctccc gaacctgttg agcaacctga aagccccgtt cacgctctcc atcaatctct
    10741 aaatccaata aatagagctg gtaatggtca aaatactgag aatacttttc aacatctcca
    10801 tagtttttgg caatatcaat ttccaatctc aaattacgag agcgaccaat atctagtaaa
    10861 gtctgctcta tccgtccttg ctggacttta tcgtcttcca aaactaaaat tctcattcct
    10921 ctccccctcg tttctcatcc ttaaatggca attctaaatc ttgtctaaag gtcgtctctc
    10981 ctagctgggt atctaagttt aaatcataat gaaaaaccat atcctccaag gttgctaagc
    11041 ccagtccact gtggtttccc ttggtagagt aacccttttg actgagaatg ctgggattca
    11101 gttcttcttt cttacgagtg ttttcaatga taaaacggac actcctatcg cctgccaata
    11161 aagcaactcg aatctgcgga ttttccgcct cactggctgc ctctaaggca tttgtcgtga
    11221 ggatagagag gatacggata gcctccaaca tcggcaatgc tagttcttct acgaccccca
    11281 ccgtctccaa actaaaatga attccctgct tatctgccac aatcatggct tttgccaagg
    11341 tattacgaat ggctatatca tggatattat gaagattaaa ggccgtgtaa tccgccttcc
    11401 tcagcttttc attggtcttt agaaagacat cttgataaat ggtagaaact tcagtcatgt
    11461 cttgattggc tatggcaggt tccatgctgg cgacaattcc tgcaaaatca tgacggaaac
    11521 ctcgcacttc atcatagaga tgacctagct tgtccaccat cccttgcaag gaacgatttt
    11581 catcttcctg tctctgaatg gtctgctcat cacgataaac ctgctccaaa tgcttgatgt
    11641 aaaagagcca agagaaaaag agaatgaaaa ttaggagaga gatggtcgtg tcaaaacgaa
    11701 cgtagaaaga actatttata ttggtcataa acgaagagaa tatcctaaac acagtcagag
    11761 gtaggataat gaaaaaactc ttacgatagt ggattttgaa agcatctgaa aagaataaat
    11821 ccaaatccaa agaccaacgc ttcattattt tacaacatag aaatattgat aataaatcaa
    11881 atacagatct atataaggtg gaaaacgaat ctacaaaaca acaaggctca ccggataata
    11941 cacgaatcca aattgtcaaa aataaataac gaatactctg aaagaagaga aaaagataca
    12001 gagaaagaaa aaaagcttcc ccttttcttt tatgcaattc taataaaaaa acacagtaaa
    12061 agaagagaat gattcctata tggaataatt ctccaaatgt ttgacgagca gataaactaa
    12121 ttaaaaatac aaggacccaa taacgttttt tata
//
