LOCUS       NZ_VFSH01000004        10297 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus shenyangsis strain D19 Scaffold4, whole genome shotgun
            sequence.
ACCESSION   NZ_VFSH01000004
VERSION     NZ_VFSH01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus shenyangsis
  ORGANISM  Streptococcus shenyangsis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 173969)
  AUTHORS   Xiao,C., Li,X., Sun,Y. and Liu,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2019) Key Laboratory of Environmental Pollution
            and Microecology of Liaoning Province, Shenyang Medical College, No.
            146, Huanghe North Street, Shenyang, Liaoning 110034, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAP denovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006385785.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 12:53:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,899
            Genes (coding)                    :: 1,820
            CDSs (with protein)               :: 1,820
            Genes (RNA)                       :: 55
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 23 of 79
            Pseudo Genes (incomplete)         :: 50 of 79
            Pseudo Genes (internal stop)      :: 25 of 79
            Pseudo Genes (multiple problems)  :: 17 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:16:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 119166
            Orig. end                         :: 129463
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VFSH01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10297
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10297
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10297
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5297](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5297)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(77..955)
                     /gene="rsgA"
                     /locus_tag="FJR77_RS04495"
                     /old_locus_tag="FJR77_04495"
     CDS             complement(77..955)
                     /GO_function="GO:0003924 - GTPase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0043022 - ribosome binding [Evidence IEA]"
                     /GO_process="GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /gene="rsgA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /locus_tag="FJR77_RS04495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04495"
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="WP_140833456.1"
                     /transl_table=11
                     /translation="MQGQIIKALAGFYYVESEGKVYQTRARGNFRKKGHTPYVGDWVDF
                     SAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHKGI
                     HPIIYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLLLLTGKVTVFMGQTGVGKS
                     TLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVTTAED
                     LNQAFPEIASVSRNCKFRTCTHTHEPSCSVKPAVEEGTIASFRFDNYLQFLSEIENRRE
                     TYKKVSKKIPK"
     gene            complement(957..1829)
                     /gene="rsmA"
                     /locus_tag="FJR77_RS04500"
                     /old_locus_tag="FJR77_04500"
     CDS             complement(957..1829)
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /locus_tag="FJR77_RS04500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04500"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_138707080.1"
                     /transl_table=11
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     DQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDILKV
                     DLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRISAQ
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVEDENLF
                     FKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLAEFASL
                     ADALKGQGL"
     gene            complement(1950..2258)
                     /locus_tag="FJR77_RS04505"
                     /old_locus_tag="FJR77_04505"
     CDS             complement(1950..2258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333266.1"
                     /locus_tag="FJR77_RS04505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04505"
                     /product="hypothetical protein"
                     /protein_id="WP_000333264.1"
                     /transl_table=11
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVNGYLL
                     DFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENR"
     gene            complement(2251..2892)
                     /locus_tag="FJR77_RS04510"
                     /old_locus_tag="FJR77_04510"
     CDS             complement(2251..2892)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /locus_tag="FJR77_RS04510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04510"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_140833322.1"
                     /transl_table=11
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLLN
                     ILGKLEKIDGGRVLYQGKDLKNIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFVGQ
                     KISKVERLERQVEALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILADEPT
                     AALDPENSEEVMNLLVGLKDENRMIIIATHNPLVWNKADEIIDMRELAHV"
     gene            complement(2894..4924)
                     /locus_tag="FJR77_RS04515"
                     /old_locus_tag="FJR77_04515"
     CDS             complement(2894..4924)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /locus_tag="FJR77_RS04515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04515"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_181146595.1"
                     /transl_table=11
                     /translation="MKRLFILISMVLVSLYMLITSVDHREEILFGNYLSVDVTGMVINQ
                     PVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGQGELPEGLTISSKES
                     AETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSSGEDPFSIVLLLAATPMVLLSLA
                     IFLLTFMSLILIYRIKSLRQAGIRLIAGESLFGVALRPVLEDMRQLIFSMLVSSLLGLG
                     ILWYQGALFMATVQLVIIGLLLYGLALVGISTLLSVVYLLGLQENSLVDLLKGKLPLKR
                     MMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASDKWSQSSDRYRLSFGWSSAFAD
                     EEGMRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLNGNRLSDYTPSGNVIYV
                     SPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYLQNFSSESV
                     EVTSQKHYLPQVTLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQATGTRPVAGML
                     WGTTANSALKLDRYGDSITALKDQGLYHKVSYLVKSQLFFAKVLNDKRMEFYSLLIGTV
                     LTLSTAILLFDSMNLLYFEQFRREIMIRRLSGMTIYELHGKYLLAQGGVLLLGLILSSV
                     LTGDSLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(5001..5297)
                     /locus_tag="FJR77_RS04520"
                     /old_locus_tag="FJR77_04520"
     CDS             complement(5001..5297)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /locus_tag="FJR77_RS04520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04520"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000815396.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 4.3e-21,
                     bitscore: 68.8, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQTVKKLALVASIAATLGGSVAVVSAAVKYPDGGVWTYGSGDGG
                     AYSNYYHSSEYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     misc_feature    complement(5664..5673)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.31"
                     /tool="antismash"
     gene            complement(5744..6655)
                     /locus_tag="FJR77_RS04525"
                     /old_locus_tag="FJR77_04525"
     CDS             complement(5744..6655)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904843.1"
                     /locus_tag="FJR77_RS04525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04525"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_140833318.1"
                     /transl_table=11
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPSL
                     PKLDYIARRLGVPAYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEIEN
                     LFYEQLPSEEKVWFEATRATIDVIRSGRSEYGETVLDDYFKTIYDKELFLINELEVINL
                     YFAIVLTKIKQGQSQISEIERIHSFLVRLANHVELISPEYLFALSNTLFSGLACLDNLS
                     TYDSLETYIFSLNHIMEKTQDFQKKPIILMLEWKLSLIIKNDYVSAEQFYQKSKLFADI
                     IENSYLVTMLEKQWQEDLKKYL"
     gene            complement(6727..7287)
                     /gene="rnmV"
                     /locus_tag="FJR77_RS04530"
                     /old_locus_tag="FJR77_04530"
     CDS             complement(6727..7287)
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /locus_tag="FJR77_RS04530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04530"
                     /product="ribonuclease M5"
                     /protein_id="WP_140833316.1"
                     /transl_table=11
                     /translation="MKEKISQVIVVEGRDDTANLKRYFDVETYETRGSAINEQDLERIQ
                     RLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEHAS
                     YEDLKTALAQVTEQFEHESQFDISRSDLIHLGFLAGADSRKRREYLGEALRIGYSNGKQ
                     LLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(7287..8060)
                     /locus_tag="FJR77_RS04535"
                     /old_locus_tag="FJR77_04535"
     CDS             complement(7287..8060)
                     /EC_number="3.1.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /locus_tag="FJR77_RS04535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04535"
                     /product="TatD family hydrolase"
                     /protein_id="WP_140833314.1"
                     /transl_table=11
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIER
                     ALELVDEYEQLYATIGWHPTEAGTYTEEIEAYLLDKLKHPKVVALGEIGLDYHWMTSPK
                     EVQEQVFRRQIQLSKDLNLPFVVHTRDALEDTYDIIKSEGVGPRGGIMHSFSGTLEWAE
                     KFVELGMKISFSGVVTFKKATDIQEAARELPLDKILVETDAPYLAPVPKRGRENKTAYT
                     RYVVDFIADLRGMTAEELAVATTANAERIFGLDSK"
     misc_feature    9540..9555
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 24.07"
                     /tool="antismash"
ORIGIN
        1 aatttcacgt tcaaagttgg cataatctgc tgccagaatt gacggagcaa tcttgtattg
       61 agacataggt ttctccttat tttggaattt ttttgctgac ttttttataa gtttctctgc
      121 gattttcaat ttcactgagg aattgcaggt agttgtcaaa acggaagctg gcaatagtcc
      181 cttcttccac tgctggcttc actgaacagg acggctcatg ggtatgggta caagtacgga
      241 atttgcagtt tcggctgaca ctagcaatct ctggaaaggc ctgattaagg tcttcagccg
      301 ttgtcacttc ataatccagc gatgaaaagc ctggtgtatc tgcgatttta cccccattga
      361 gattataaaa actaacagct cgagtggtat ggcgcccgcg acctaaacta tcagaaattt
      421 ctcctgtttc aagattgagg tctggtgcga ttttattgag aagagttgac ttcccaacac
      481 ctgtctgccc cataaagact gtaaccttgc ctgttaacaa gagcagtagt tcctctttac
      541 tggtcacaaa gtcatagccg atatcaccat aggtctgttg gtaaaaatcc agttcccctc
      601 tatcttccag taggtccatt ttagaaatat aaatgatagg atgaataccc ttgtgctcta
      661 aaagaaccaa aaaccgatcc aacaaattgc tgttaaaatc aggttccttg acagacatga
      721 tcaccacagc ttggtcaata ttgacaatag gcggacggac cagactgttt ttccgttcgt
      781 gaatcttgag aatatagcct tctgaatttt cttcggcaga aaagtcgacc cagtccccaa
      841 cgtaaggggt atgacctttt ttacggaaat ttccacgcgc tcgtgtttga tagactttgc
      901 cctcactctc tacatagtag aacccagcca aggctttaat gatttgtccc tgcatcttag
      961 agtccttgcc ctttaagtgc atctgctagg ctggcaaatt ctgccaagct gagagcttcc
     1021 ccacgcacac ttggtgacaa acctgcttgg tccaaggcct tggtcagctt gtccttgact
     1081 tcttcggtct taccaaagta acctgtcagg ttattccata aggtcttgcg acgatgagtg
     1141 aaacttgcct tggaaacctt aaagaataag ttctcgtctt ctactgctac ggctggttct
     1201 ggacggcgca ccattttcaa gatggctgag tccacatttg gtgctggcac aaagaccgta
     1261 cgaggcacga taaaggcaac cttggcagtc atgtaatact gcaccgcaat cgacaagcta
     1321 ccgtaagcct tggtattcgg ctgagctgaa atgcggtccg ccacttcttt ttgcatcatg
     1381 acgacaaact cactaaaagg aatgccgctc tcgatcaagt gcatgagaat aggcgtcgtg
     1441 atgtaataag gcaaattagc cactaccttg atgggcaggt caggattttt gaaattctgg
     1501 atatgttgcg ccaaatcaac cttgagaata tcttcgttaa ctacggtcac attatcaaaa
     1561 tcacgcaagg tatctgccaa aattggcacc aaacggtggt cgatctcaaa agccatgacc
     1621 tctgctgcac gctcagccag aaactccgtt aaggcaccaa tacctggccc aatttcaata
     1681 acattgacct ggtcatcaat ctcagccgta tcgacaattt tttgaaggat attagtatca
     1741 gtcagaaaat tttgtccgaa agacttttta aaggtaaaac cgtgacgctc cagcactgcc
     1801 ttggtcacgc tataatctgc aattctcatc tattctcttt tctattatct gttactacta
     1861 ttgtaacaca ttccccttgt attttggggg tgaaaatcat accaaaaaac tatctttcct
     1921 catgaagaaa gatagtcttg gaaatggact tatcggttct ctcgaaacat acgttcaaaa
     1981 aaatcagctt cttgataact tcgattttca ggtagggtca aatctacatc ctcctccaca
     2041 tcgtccactg tcagttcaac cgtttgaggt aaatttttca aacgataatg cttttgcctt
     2101 tgcccttcaa tgctagcaaa atcaagcaaa tagccattta ctcggatttg aaccaaaagg
     2161 agatgattgg tcatattttc catgatgaga atatcttcta aaccacctct ggcactagga
     2221 taatcagata ccctgcgaat acgaattttt tcacacatga gcaagctccc tcatatcaat
     2281 gatttcatcg gccttattcc agactagggg attatgggtc gcaatgatga tcattcgatt
     2341 ttcatctttc aagcccacca atagattcat aacctcctct gaattttcag gatctagagc
     2401 tgctgttggt tcatccgcta aaatcaaggg tggatttttc aaaatagtct tcgcaagggc
     2461 aactcgttgg gcctctcccc cagataaagt ataaattttt tgttctagat ccaaataccc
     2521 tagattaacc ttttctaaag cctccacttg cctttccaaa cgttctactt ttgagatttt
     2581 ctgaccaaca aagcccaaat ccaaattctc tttgattgac tggttttcta aaaggccaaa
     2641 attttgaaag agatagccca tctggtctcg aaaatactca cgagtgggaa tgttttttaa
     2701 atctttcccc tgatagagaa cccttccacc atctatcttt tctagctttc ctaagatgtt
     2761 cagcaaagtc gtctttccgc ttccactctt tccgattaag gcataaacct tgcccttctc
     2821 cagcttgaga tttaaaccag agaaaatcgc acggtcatta aacttctttt ccaatccttg
     2881 aatatcaatc atcttatttt cctttcaata ctgccatgct accagcttct tcctttttat
     2941 cctgccttac taaaatcaag agggcgttaa gagtaaataa agctacaact agagcgctaa
     3001 tcaagctatc tcctgtcaaa acactagata ggattagccc aagcaagaga actcctcctt
     3061 gcgccagtaa gtacttgcca tgaagctcat agattgtcat accagaaaga cgtctaatca
     3121 tgatctcccg tctgaactgc tcaaaataga gaagattcat ggaatcaaat aacaaaatag
     3181 ccgtagacag agtcaaaaca gtcccaataa ggagagagta aaactccatc cgtttgtcat
     3241 ttagtacctt ggcaaaaaat agctggcttt ttaccaagta agagaccttg tgatatagtc
     3301 cttgatcttt tagagctgtg atgctatctc catatcgatc tagtttcaag gcactattag
     3361 ccgtagttcc ccacaacatc cctgcaacag gtcttgttcc agtcgcttgt ggcgtgagaa
     3421 ctacaataat cgggtctttt aggtactggc gtgtcgtctt gtacccatcg ttatagagga
     3481 agcgttcctg tcctgtttct gtaaaagcta gcgttacctg tgggaggtag tgtttctgac
     3541 tcgtcacttc tacactttca gatgaaaagt tttgcaggta atctgtaaat aatccttgat
     3601 agtaggcaga ttgctctcgc aagctttcag gcaagatcag cccaaactct ccctcagaca
     3661 agttttgcat cttgtccata aactctgaag aaacggtaat cttttcttct atcagatagc
     3721 gcggtgagac atagataaca ttccctgacg gtgtatagtc actgagtcga ttgccattta
     3781 gatccacttc tgcgccatct gagaaattgt caacattgct catgatataa aaagagtctg
     3841 tattggctaa ccgttcttca gtaaatgtct gccactcacg attgtcctta cgcattcctt
     3901 cttcatcggc aaatgcacta gaccaaccaa aggatagacg gtaacgatct gaggactggc
     3961 tccatttatc actagctctc tccatttcct gcatttcacg gtagtggggt aggagagcag
     4021 tcgcactcga gccgacaacc aatacagcta aaagttgccc caccatcatc aatgtcatca
     4081 tacgtttgag agggagtttc cctttcaata gatccaccag gctattttcc tgtaaaccaa
     4141 gtagatacac gacacttaat aaggtagata tccctaccaa ggccaatcca taaagcaaaa
     4201 gaccaatgat gaccagttgc actgttgcca taaacaaggc accttgatac cagagaatcc
     4261 ccaatcccaa aagactggat accagcatcg agaagataag ctgtctcata tcttctaaca
     4321 ctggtctgag agccacccca aacaagctct caccagctat taagcgaatc cctgcttgac
     4381 gaagggattt tatccgataa atcagaatca aactcataaa ggtcagcaga aaaatagcta
     4441 aactcagtag caccataggg gtggccgcca atagtaagac tatcgaaaat ggatcttctc
     4501 cactcgaaac aaagccttga tagccaagtt ctttcaaggt ggtctgtaag ctcactccat
     4561 ccaaacttcc tgacacaatc aagtaagacc ctaataaatc actcgtttct gcactctcct
     4621 tggaggaaat ggtcagacct tctggaagct ctccctgacc gtaagtggca taggtaaagc
     4681 gtgtttctcc agcttcattt ggctcaacaa tccgacgggc gatgagacta ttatgctcta
     4741 ctgccaagtg actcaaagcc tctgtcacct cttcgcgact agctactggt tgatttatta
     4801 ccattcctgt cacatcaaca gagagatagt taccaaataa aatctcttct cgatggtcaa
     4861 cggaagttat cagcatataa agcgatacca ataccattga aatcaaaata aataaacgtt
     4921 tcatcatttc ctccaaaaag ttacttggtt acttagtgag aaggtgatca agtaaagcta
     4981 aacaataggg ctataccatt ctaaacatta tagtagaatg caactttctc tccccaagaa
     5041 gtacgaatcc atgcacgaga agtccctcca gcaccagcat atcccttatc agatgaacca
     5101 gtttttctac taacaactgt tgagctatga tattctgaag agtgatagta gtttgagtaa
     5161 gcacctccgt cacctgaacc atacgtccag acaccaccat ctggatactt aaccgcggcc
     5221 gatactactg ctacacttcc acctaatgtt gctgcaatgc tcgctacaag ggctaatttt
     5281 ttaactgttt gtttcatatt gatactcctt tattttttat tttatcataa atatttatta
     5341 tatattgtcc attttctatt caagtaaaac taagtcatgg atcatagacc ttttcaaagc
     5401 aacacctcat ggcatcctct ccttattttt ctaatttagt ctatcaaatt atatttatta
     5461 tatattatga ataaattttt gagactaagt agacataact aaattttatt taaaccacca
     5521 agtaactaaa taaaataaac taaaaaagtt catactgtaa tctcctttca cgttttaatt
     5581 aattataaat tattcttaaa taaactcaaa ggacatcaat gtccttaaaa tataagcatt
     5641 tcatgtcatt tttagcttat atataaaaaa acagccctaa agacgaactt tagaactgtt
     5701 tcaagatcag aacaaggaca tttttgtcac tgcttcacta tgtttataaa tattttttta
     5761 aatcttcttg ccattgtttc tccaacatag taactaaata agaattttct attatatcag
     5821 caaatagctt cgatttctga tagaactgtt cggcagagac ataatcattt tttattatta
     5881 gggataattt ccactccaac attaatataa ttggtttctt ctgaaaatct tgtgtttttt
     5941 ccattatgtg attaaggcta aagatatagg tttctagtga atcataagtc gataaattat
     6001 ccaaacaggc aagacccgaa aacaaagtat tacttaaagc aaacagatat tctggtgaaa
     6061 ttaattccac atgatttgcc aaacgaacta aaaacgaatg aattctttca atttctgaaa
     6121 tctgactttg accttgtttt atctttgtaa gcactatagc aaaatataag ttgataactt
     6181 ctaattcatt tatcaaaaac aattccttat cataaattgt tttaaaataa tcgtccagta
     6241 cggtttcccc gtattctgat cgtccgcttc gaataacatc tatcgttgct ctagtagctt
     6301 cgaaccatac tttttcctca ctaggaagtt gctcataaaa caagttctct atctcttcca
     6361 aacacgcttc tttcttatcg tattcctctt ctttaccata gattggttca cgtaaaatct
     6421 ggtatttcaa ttctaaataa gcagaaggaa gagctgaaaa atccggcata aggctataag
     6481 ctggaactcc taaccgacga gcaatatagt ctaacttcgg caaactcggt tgcgaagctc
     6541 cactttcaat tcgcgacagt tgacgaactg tcagttcttg ctcatctcca caaaaatctg
     6601 gacgactaat tcctttttct atccgaatct ctttaatttt ttgaccaatt aacatcagac
     6661 gtctcctctt ctacctgctt cgaaaaatac tgtgaaactt aaaaaattgt aacgttcaca
     6721 tctttcctac tcataacttt tcatagcttc ttccacttct gctaaagtaa ccccaaacaa
     6781 ctctaagcgt ttgaggagtt gcttgccgtt agaatagccg attcggagag cctctcctag
     6841 atattctctt cgcttacggc tgtctgctcc cgctagaaag ccaaggtgaa tcaagtcgct
     6901 acgactgatg tcaaactgac tctcatgttc aaattgttct gtaacctgag ctagagccgt
     6961 tttcaggtct tcatagctgg catgctcaat tcccagggaa cggcccttgg tcttggactt
     7021 gggaacagct tcatctcgtt tgaggaaggc atgctgaact gttggaatgg ccgtcataat
     7081 catgcgacga atccgctccc cattaaaatc tgggtctgta aagacaatga ccccatgacg
     7141 ctggtgcagg cgctgaattc gctctagatc ctgctcatta atggcagaac ctctagtctc
     7201 ataggtctct acatcgaaat aacgtttgag attggccgta tcatcgcgac cttcgaccac
     7261 gatgacttgt gaaattttct ctttcattac ttactgtcca atccaaaaat gcgctctgca
     7321 tttgcagtcg ttgctactgc tagctcttct gccgtcatgc cacgcaagtc agcgataaag
     7381 tccactacat agcgagtata ggctgttttg ttttcgcgac cacgcttggg tacaggtgct
     7441 aagtaaggcg catccgtttc caccaaaatc ttgtccaaag gaagttctct agcagcctct
     7501 tggatatcgg ttgccttctt gaaagtcact actcctgaaa aggaaatttt catgccaagc
     7561 tcgacaaact tctctgccca ttcaagagtt cctgagaaag aatgcatgat accacctcga
     7621 gggccaactc cctcgctctt gataatatca taggtatctt ccagcgcatc acgggtatgg
     7681 acaacaaaag gtaaattcaa gtccttagat aactgaatct gacgacgaaa aacctgctcc
     7741 tgcacttcct taggcgatgt catccaatga taatctaagc caatttcacc tagagccaca
     7801 accttgggat gcttgagctt gtcaagcaag taggcttcaa tctcctctgt gtaagtacca
     7861 gcttctgtag gatgccaacc aatagtcgcg taaagttgct catactcgtc taccaactcc
     7921 aaggcacgct caatcgtcgg tttatcaaaa cccacaatat tcatctgcgt aacccccatc
     7981 tcagcagcca aggcaatttc ttctgcctca cgacctgcaa attcttctac attcaagtgt
     8041 gtatgtgtat caaaaatcat ctcttctaac ctcgttttct atcttctatt atagcaaaaa
     8101 aactgacctc tctcctcatc tgaaaaaaat attctaaaac ccatcatcat ctcttgatgc
     8161 tcttttttca aagcaatata atagcactta ttgaattttt caacaaagta aatgaccatg
     8221 tttacaaaat taaaatagcc cctcatagat cgtcctccta agtccttaac cgaaggctag
     8281 gggagactgc tgggaaaact ccgatactgg gcatcttgtc aagcggcgct ctgtcctcca
     8341 tcgcctatgg gccttagcaa gtagtcctaa tatcccagtt tatctctaat aaaccttggc
     8401 agaatatcct gcacaatcag atgagtctca aactcaaata cgttcgtcgt tggcaccaag
     8461 acgtcgttgt cgctagctac tcctatcact taaaagaata aagaaaaggc cctaccagaa
     8521 ccaataagca tctggtaagg cctttttctg aaacaagtca ctatgcttat actcaatgaa
     8581 aatcaaagag caaactagaa gctagccaca ggctgctcaa agcactgctt tgaggttgta
     8641 gatgaaactg acgaagtcag taaccatata tacggtaagg caacgctgac gtggtttgaa
     8701 gagattttcg aagagtatta actgaaaagc atgactagga atatcattga atgtgatttg
     8761 ctaaatcttc ctgagctttt ttatttgact cagctttttc ttttagccat ttttcataga
     8821 gttcttggta ctgtttagca gttactggct ctttttgcaa ttcaacatat ttatagttgt
     8881 ctgcatttcc cttatgacca acgaatgaga atggacctgt aaacggtaca gtcttacgga
     8941 aaattgggtt ggctccgcca ctttgaactg gtaggaagag ggcactatct gtcaaccaag
     9001 cttgggcttc agcgtatttt tcataacgag cttgagtatc tgtgatttca gcatcagcct
     9061 gatccaagag tttcttgtag tcagaaagac caatcttgtc cttaatttcc ttgtcttttc
     9121 cttcagtcaa gcccatgttt ttcaactgag aacctgtctc tggatcaagg atggctaggt
     9181 aagtcttcgg atctgagtag tctccacccc atcctgagat gtcgatatcg taatcttttt
     9241 gttctgctgt atctgcaaag taggttgcct gatctttttc atctggagaa agttggataa
     9301 catcgatgac aacattatcc ttaccaagag tggcttctac tgtttgcttg agagagccag
     9361 cctgttgcac atctagctta gcagtctggt caactggcat atccaaatga attgggaagg
     9421 ttactccctg agcttgaaga ctttctttag ccttagcaaa tttctctttg gccttgtcag
     9481 ggttcaagaa ggcttgctta ccatcgttta ggttgatatt tgcccaatct ttaccgtagt
     9541 tgacaatttt ttcattgaca atatcaccga agttcttatc gccaacttgg acaaagttac
     9601 ttggtgtaat cgtgttacgt aagatacggt ctgctccatc ttcaccattt gtctgtgctg
     9661 cataagcatg acggtcaaag gcaaagttga tagcctgacg gaaatcctta ttttgcatag
     9721 ctgcgcgtga atctgtcttt tccttatcag actgtttcat agtctgcttg tagctttgac
     9781 gattcacatt gaaaagataa tagaaagtaa ctgaattttg cggtgtatag gtaatcttgt
     9841 cctcatttcc cttcttaagc tcagaaaaga ctgaacttgt tgggaagata cggccatcag
     9901 tatagttacc atccaagaaa cctcttgcaa tgctgtcttg gtcagaaccg tcaaagaaag
     9961 aaagtttcac tttctcgatt ttaacattgt ccttatccca gtaattaggg tttttatcaa
    10021 gttcaatcaa agattttgat gtgaatgatt taaataagta aggaccattt gacaagatac
    10081 ttgatggtgt cacacttcca aagtcatctc ccttagactt caagaaagct tcgtttaccg
    10141 gactaagaat acttgctgtt gttttagagt tccaataagt ctcaggctgg ttgagagtgt
    10201 attgtagcgt gtaatcatcc accgccttaa caccaacagt tccaaaatca gatgattgcc
    10261 cttcaacata gtctgctaac cctttaatag agtcttg
//
