LOCUS       NZ_VFSH01000004        12154 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus shenyangsis strain D19 Scaffold4, whole genome shotgun
            sequence.
ACCESSION   NZ_VFSH01000004
VERSION     NZ_VFSH01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus shenyangsis
  ORGANISM  Streptococcus shenyangsis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 173969)
  AUTHORS   Xiao,C., Li,X., Sun,Y. and Liu,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2019) Key Laboratory of Environmental Pollution
            and Microecology of Liaoning Province, Shenyang Medical College, No.
            146, Huanghe North Street, Shenyang, Liaoning 110034, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAP denovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006385785.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 12:53:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,899
            Genes (coding)                    :: 1,820
            CDSs (with protein)               :: 1,820
            Genes (RNA)                       :: 55
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 23 of 79
            Pseudo Genes (incomplete)         :: 50 of 79
            Pseudo Genes (internal stop)      :: 25 of 79
            Pseudo Genes (multiple problems)  :: 17 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:16:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 24442
            Orig. end                         :: 36596
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VFSH01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12154
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12154
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12154
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7154](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7154
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(610..888)
                     /locus_tag="FJR77_RS04060"
                     /old_locus_tag="FJR77_04060"
     CDS             complement(610..888)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042208.1"
                     /locus_tag="FJR77_RS04060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04060"
                     /product="hypothetical protein"
                     /protein_id="WP_000042207.1"
                     /transl_table=11
                     /translation="MSKNSRTEKARVTESADFGAYFLILAVVAVGALAYFSEDFGPRWA
                     KVIEIVGAPFSLGLGFFAFDKGFEMLNDYKKNKRKEDDEEEKNNNQN"
     gene            complement(1079..2116)
                     /locus_tag="FJR77_RS04065"
                     /old_locus_tag="FJR77_04065"
     CDS             complement(1079..2116)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744512.1"
                     /locus_tag="FJR77_RS04065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04065"
                     /product="thioredoxin domain-containing protein"
                     /protein_id="WP_000795770.1"
                     /transl_table=11
                     /translation="MKNQKIKSLLLVPLVFASITTGVVYAEEQPTTSSIQANDSSVVTP
                     DLPSAKEKVEHKPSVVTPEEYEKNVTDFKKIDIEAVRQSFTEDQLEHTIYFGRKTCSHC
                     RQFSPELKEFNNLIEKKLEYYDLDGKDFDGEAREFLFKKVGIPGTPTILYLKNGHPISG
                     WVGGGATAQQVYDYMYSRNSPKQTETVKPSEETVTESVRDEKTRNDSMSISNKVVSDSK
                     GMSDEKRTEMKISNDNPNSNSENSGKVESTNSTSKAKTTQSGNLAMLDNKNQLETPKPI
                     FDVAQENKTPLTDVKDADYVSESITSNEKLLPKTGEEESYQLIWLAVAFFMASIMIKVK
                     QKIYK"
     gene            complement(2277..2681)
                     /locus_tag="FJR77_RS04070"
                     /old_locus_tag="FJR77_04070"
     CDS             complement(2277..2681)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /locus_tag="FJR77_RS04070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04070"
                     /product="immunity protein"
                     /protein_id="WP_140833403.1"
                     /transl_table=11
                     /translation="MKKIFFKKRTIFLARLFLGQFPVLVSTYLFLSRQFLNFSVVFQFL
                     LVVINLASILATVYLTREMRIREFEDDDFVSPRTNQLMFIGLTGFMSIICLYRGIIATE
                     AYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
     gene            complement(2710..3024)
                     /locus_tag="FJR77_RS10285"
                     /pseudo=""
     CDS             complement(2710..3024)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077227.1"
                     /locus_tag="FJR77_RS10285"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="role in replication"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSQFEIVDTENFAKVEEGFGGWEEEAFQKNYMV"
     gene            complement(3121..3723)
                     /locus_tag="FJR77_RS04080"
                     /old_locus_tag="FJR77_04080"
     CDS             complement(3121..3723)
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151808.1"
                     /locus_tag="FJR77_RS04080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04080"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000151808.1"
                     /sec_met_domain="Abi (E-value: 5.2e-19, bitscore: 61.9,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIFNKCHAVFVGFLAFITVTTVGYTINQGDFFQNEYLFIITKTF
                     WISLSVAYAKWFDMVSLRRLTKKEILLFIGSFLICVLVNIGYYSFFTVSSGARYQHLEA
                     TSTGISLSFIASVTVFGPILEEFVFRGILQGAVFENSWLGLILTASLFSFLHAPYDVPS
                     FIYYLFGGFMLGFAYKKSQNLSVPILVHICYNCLSFL"
     gene            complement(4105..4302)
                     /locus_tag="FJR77_RS04085"
                     /old_locus_tag="FJR77_04085"
     CDS             complement(4105..4302)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FJR77_RS04085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FJR77_04085"
                     /product="hypothetical protein"
                     /protein_id="WP_000521218.1"
                     /transl_table=11
                     /translation="MGKVTIIELSELELIYGGDSHGSIFHPDTWVRYFEYALTHPGKGG
                     NLPLCENYTPAYPGKILPQC"
     gene            complement(4312..4506)
                     /locus_tag="FJR77_RS04090"
                     /old_locus_tag="FJR77_04090"
     CDS             complement(4312..4506)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FJR77_RS04090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FJR77_04090"
                     /product="hypothetical protein"
                     /protein_id="WP_000874095.1"
                     /transl_table=11
                     /translation="MKYIKVLTDSNLERIQGGDDLINFYELGEKIGRYLAHPHPNSVTL
                     PMCYEYGPAYPAPVLPCRK"
     gene            complement(4523..4708)
                     /locus_tag="FJR77_RS04095"
                     /old_locus_tag="FJR77_04095"
     CDS             complement(4523..4708)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603122.1"
                     /locus_tag="FJR77_RS04095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04095"
                     /product="hypothetical protein"
                     /protein_id="WP_000603122.1"
                     /transl_table=11
                     /translation="MILQSSVSLKILDEDTLSDIEGGIAWWVGPAGFFAYGILAGYTDE
                     KCMIDNGNHWYCVKLP"
     gene            5001..7154
                     /gene="blpA"
                     /locus_tag="FJR77_RS04105"
                     /old_locus_tag="FJR77_04105"
     CDS             5001..7154
                     /codon_start=1
                     /gene="blpA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /locus_tag="FJR77_RS04105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04105"
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="WP_000205163.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.2e-42, bitscore:
                     137.5, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1116.2,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKTN
                     KEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQTK
                     KDYLIIGDPDPSVKITKMSKERFFSEWTGVTIFLAPKPSYQPHKDKKNGLLSFLPLIFK
                     QKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMRSTLGIISVGLVITYILQQVM
                     SFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSIIDALAS
                     TILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMVIIFSFMKPFEKMNHDVMQSN
                     SMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQTSLKQGTK
                     LVLNILILWFGAQLVMSSKISIGQLITFNTLLSYFTTPMENIINLQTKLQSAKVANNRL
                     NEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIKQGDKVSLVG
                     VSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQAYIFNGSILE
                     NLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGGQKQRIALARAL
                     LTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRLSIAERTNRVIVLDQGK
                     IIEVGSHQELMQAQGFYHHLFNK"
     gene            7165..8526
                     /locus_tag="FJR77_RS04110"
                     /old_locus_tag="FJR77_04110"
     CDS             7165..8526
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /locus_tag="FJR77_RS04110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04110"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_140833400.1"
                     /transl_table=11
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLLTFILIFSLVATKEI
                     TVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTALET
                     QLHRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFRNFTKQSHDIELGITKTNTEV
                     SNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRYLVAS
                     QGQTQGTADEPFLSQINQSIAGLETSIASLKIQQAGIGSVATYDNSLVTKIEVLRTQFL
                     QTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIIHLNSEFEGKNRIPTGTEI
                     AQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGITIIGQLQTIDQTPT
                     RTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKMLTHSD"
     gene            8583..8738
                     /gene="blpC"
                     /locus_tag="FJR77_RS04115"
                     /old_locus_tag="FJR77_04115"
     CDS             8583..8738
                     /codon_start=1
                     /gene="blpC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /locus_tag="FJR77_RS04115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04115"
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="WP_138705438.1"
                     /transl_table=11
                     /translation="MDKKQNLTSFQELTTTELNQITGGGWWEDLLYRFNLIEQNNTKGF
                     NQPIQL"
     gene            complement(8781..10121)
                     /locus_tag="FJR77_RS04120"
                     /old_locus_tag="FJR77_04120"
     CDS             complement(8781..10121)
                     /EC_number="2.7.13.3"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /locus_tag="FJR77_RS04120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04120"
                     /product="sensor histidine kinase"
                     /protein_id="WP_140833398.1"
                     /transl_table=11
                     /translation="MNIAWILLYSLIINGLKIVIFFKVDGISLTFDRIFKAFLLKFLLA
                     AIFTTFKFLVLTDYLSYLIEPLFGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRGVS
                     YFVLPFLGQEIVDKDSNPIFLLIMIFVCFIVLVFLKWLDYDFTRLRREILDKDFQKSLT
                     KINWAMGAYYLVMQSLSYLEYEQGIQLRTVRHFILVFYLLFFMGIIKKLDTYLKDKLRE
                     RLNQEQALRYRDMERYSRHIEELYKEVRGFRHDYTNLLTSLRLGIEEEDMEQIKEVYDS
                     VLKDSSQKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKKIVFNVEVPEEIQVEGMS
                     LLDFLTIVSILCDNAIEASAEATQPHVSIAFLKNGAQETFIIENSIKEEVIDLSEIFSF
                     GASSKGEERGVGLYTVIKIVESHPNTSLNTTCQNQVFRQVLTVIPIE"
     gene            complement(10135..10872)
                     /locus_tag="FJR77_RS04125"
                     /old_locus_tag="FJR77_04125"
     CDS             complement(10135..10872)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /locus_tag="FJR77_RS04125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04125"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_140833396.1"
                     /transl_table=11
                     /translation="MRIFVLEDDFSQQDRIETTIEKLLKEHHITPSSFEVFGKPDQLLS
                     EVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQVS
                     ALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYYLET
                     SPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKLLFFP
                     NGGSCMIARYKVREVSEAINNLH"
     gene            complement(10877..11215)
                     /locus_tag="FJR77_RS04130"
                     /old_locus_tag="FJR77_04130"
     CDS             complement(10877..11215)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /locus_tag="FJR77_RS04130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04130"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_000682137.1"
                     /transl_table=11
                     /translation="MKHMIIQTQKTVYKVNVDDVYYIQTHPTKAHTVQIVTEEASFNMV
                     QNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYREIR
                     QKWLKEGE"
     misc_feature    complement(11342..11355)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.71"
                     /tool="antismash"
     gene            11577..11828
                     /locus_tag="FJR77_RS04135"
                     /old_locus_tag="FJR77_04135"
     CDS             11577..11828
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130045.1"
                     /locus_tag="FJR77_RS04135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_04135"
                     /product="hypothetical protein"
                     /protein_id="WP_001130044.1"
                     /transl_table=11
                     /translation="MAKSNKTKQLTSIELSHINGGSIPYGWYSPFHIISDNVLCRNGYS
                     YNKANLDNGKCIIDWGNIVGNVANNIGASLGDLHNPLP"
ORIGIN
        1 caggcgtcct ctctattctg ttgataagac ggagtaggga tataactgtc aactgaatca
       61 gaagtatgga ttctcctctt attaatgaag tcagttggtt ttcaactgca tataaaaaga
      121 ctggtagcat aacacaggct atagcaatca caaacaggtt gctccccgtt tcttctccct
      181 tgttcaccac ggaaatcatg agaagatttg atgctatgat caacgtagaa cagatgataa
      241 aaaaggattt cttattcata taggatacct caaataatat aacatccaac ttattaaaag
      301 tcaggctgga taaagagaaa atttatctgt tagcttctgc aaattgaaac attgtttttg
      361 tattcacgag gaatactttt tttattttcg tcctgttaga atatcgtatg cttgtactcc
      421 atcaagcaac cccattccat tagatagtat gttgtctaac tcctccatct tttcatctga
      481 aaatgttatt tttcagcgtt gattactttt tcataggttg ctatcgtcct tgacaacacc
      541 atcttcccaa actcttctcc aagtaacttc tttttactcg ccatttttta cccctttatt
      601 ttacattttt tagttctgat tgttgttttt ttcttcctcg tcgtcttctt ttcttttatt
      661 ctttttataa tcgttcagca tttcaaaacc tttatcaaaa gcaaaaaaac ctaaacctaa
      721 agaaaacggc gctccgacta tttctataac ttttgcccac cttggcccaa aatcttctga
      781 aaaataagca agcgccccga cggctacaac tgctaaaatt aaaaaatatg ctccaaaatc
      841 tgcactttct gttactctag ctttttctgt tctactattc ttactcatga taattttcct
      901 ttcattttcg attttctact tcagctaata ctaactattt accattcatt tcatatacag
      961 ttataatctt ttatatataa actaaataca cctatcttct cactcttgtc tataggcaaa
     1021 ggttagccat atgttattac aaacaaataa tatcattgca cctgtttacc atctattttt
     1081 atttataaat tttttgtttc actttaatca ttatagaagc cataaaaaat gcgacagcca
     1141 gccaaatcag ttggtaactt tcttcctctc ctgttttcgg taataatttt tcattcgaag
     1201 taatagattc gcttacataa tcagcatcct taacatcggt taaaggagtt ttattttctt
     1261 gagcaacatc aaaaataggt ttgggagttt ccaattgatt cttattgtct aacattgcta
     1321 aattgccaga ttgagtagtt ttagctttag aagttgagtt tgtactctcc actttcccac
     1381 tattttcact attagaattg ggattatcat tggatatttt catttcggtt cttttttcat
     1441 cactcatacc tttgctgtca cttacaactt tattactaat actcatgctg tcatttctgg
     1501 ttttctcatc acggacactt tcagttactg tttcctcaga aggtttcact gtctctgttt
     1561 gcttaggtga atttctcgag tacatataat catacacctg ttgtgcagtt gctccgccac
     1621 caacccatcc agatatcgga tgtccatttt ttaaatacaa aattgttggt gttcctggaa
     1681 taccgacttt tttaaataga aactctctcg cttccccatc aaaatcctta ccgtccaaat
     1741 cataatactc taacttcttt tcaatcaagt tattaaattc tttcaattca ggagaaaatt
     1801 gacgacaatg agagcaagtt tttctaccaa aataaatagt atgttccaat tgatcctctg
     1861 taaaagattg acgtacagct tcaatatcta tctttttaaa gtccgtaaca ttcttctcat
     1921 actcttctgg ggttacaaca gaaggtttgt gctccacctt ttccttagcc gatggcaaat
     1981 ctggtgtcac aacagaacta tcattcgcct ggatagatga agtagtaggc tgttcttctg
     2041 catatacaac acccgtagta atagatgcaa atacaagtgg tacaagtaat agcgatttta
     2101 tcttttgatt cttcataaga atactccttt acatttgtta tttttatctt accttattta
     2161 agattacaaa ttcatataaa tcttaaatgg tcatcaaaac atcttgaatt gcaaaaactt
     2221 cattataaaa gggtaagtat ttcgaaaaca cttacccctt tatactttat taagctctac
     2281 tttttataat acttcaagcc ccacataagc agaagcatga taattaagca gagaacagcg
     2341 ccaatatatg cgattatttg ttggtaggct tctgttgcta tgatacctct atacaaacaa
     2401 ataatagaca taaagcctgt caagccgatg aacatgagtt gattggttct aggactaaca
     2461 aaatcatcat cttcaaattc tcttatcctc atttccctag tgagataaac agtagccaaa
     2521 atagaagcca agttaataac tactaaaaga aattggaaaa ctacggaaaa atttaaaaac
     2581 tgacgagata gaaacagata agtagagaca agcacgggaa actgacctag gaacaatctc
     2641 gcaaggaaga tggtccgttt tttaaaaaat atttttttca ttgctttctc cattacaatt
     2701 aaatgacatc taatcatttt ctaaaatttg ataacttttg tgaaatgttt tagcagaaac
     2761 aactctatga tttgtaaggt caagataatc tcctagatag ccaagaacat acattccata
     2821 accagcgaat cctcctacaa ctgttccaat cagactaaac ttgatattag ttacgaaaaa
     2881 agtattaaga accaccatta gaatgcgcag atgattatcc aatcaaacca tataattttt
     2941 ttgaaaagct tcctcttccc aacctccgaa accttcttca actttcgcaa aattctcagt
     3001 atccacaatc tcaaattgtg acatcattta atattattaa tctaaacagt aatagtatcc
     3061 atttccctat tttttaagat tttttatcca atattatatc atattggtta cttacatata
     3121 ctacaagaat gataaacaat tgtaacagat atgaactaaa ataggcaccg atagattttg
     3181 actcttttta taagcaaagc ccagcatgaa acctccaaat agataataga taaacgaagg
     3241 aacatcataa ggagcatgca ggaaagagaa gagagaggca gtcagtataa gccccaacca
     3301 agaattttca aaaaccgcac cttgaaggat acctctgaaa acaaattcct ccagaatagg
     3361 cccaaaaact gtcacgcttg caataaagga taaggaaatt cctgtactag tagcctcaag
     3421 gtgttgatat ctagcaccag aagagactgt aaaaaaacta taataaccta tatttaccag
     3481 cacacaaata agaaagcttc caataaaaag tagaatttct ttcttcgtta accttcttag
     3541 cgaaaccata tcaaaccatt tcgcataggc aacactcagt gaaatccaga aagtctttgt
     3601 gataataaat aggtactcgt tttgaaaaaa atctccttga ttgatggtat aacctactgt
     3661 agtaactgtt ataaaggcta aaaatccaac aaacacagca tgacatttgt taaaaatagt
     3721 cataaataca acctctcttt aattgaagta actaaaaact taaacttcaa aatttttttc
     3781 tctataaata cattttactg atattccaaa catagacaat ctaaattttt gtgaatttct
     3841 acaagaaaat tggaattttt caaatcatta cgagcaaagt ttgaaactaa caaactcctc
     3901 caccttacta taaagcacca gaaaacggct aatttcaata cttaggatat ttgagactat
     3961 ataatcgaat ctaagtttag aatttccttt ttttctagag tagttcctca gataacttta
     4021 cctatcattc gaatttacct aaacacagta taataaaact ttgtctttaa cctgaggtgt
     4081 tttcaactat agttttataa aaatttaaca ttgtggtaaa atttttcctg gatacgcagg
     4141 tgtatagttt tcacataaag ggagattccc tccttttccc gggtgagtta aagcatattc
     4201 aaaatatctc acccaagtgt caggatgaaa tattgaacca tgactgtcac caccataaat
     4261 caattctagt tccgataatt ctataatagt aacttttccc attctatttc cctactttct
     4321 acatggtaaa acaggcgccg gataagctgg accatattcg taacacattg gaagagtaac
     4381 cgaatttgga tgagggtgtg ctaaatatct tcctattttt tctcccaatt cataaaaatt
     4441 tatcaaatcg tctcctcctt gtattctttc taaatttgaa tctgttaata cctttatata
     4501 tttcattgtt ttactccttc ttctatggta gcttaacaca ataccagtga tttccattat
     4561 caatcataca tttctcatca gtataaccag ctaatatccc ataagcaaaa aatcctgctg
     4621 gaccaaccca ccaagcaatt ccgccttcta tatcagaaag tgtatcctca tcaagaattt
     4681 ttaaggatac agaagattgt aaaatcatga tgaatacctc cattttattt ttatttttat
     4741 tttttgtctt gttgcaacct tgacaagttt agtatatcac cgtttattaa tttttttcat
     4801 ccaaatcttg aattgtcatc gaaacgtctt aaattagctt ttttatttaa aaccacctct
     4861 aaattttttt taaagataat ttctaatcac ttttttacca ttcaggaagt ttcaatgact
     4921 attcaagatt tcataaaata tgaacttatt tttatggcat aatataccta tctactatat
     4981 gaaaatgaaa ggaattgcca atgacttctt ataaacgtac atttgttcct caaatagatg
     5041 ctagagactg cggtgttgct gccttagcct cgattgctaa attctatggt tcagattttt
     5101 ctctagctca cttaagagaa cttgcaaaga ccaataaaga agggacgact gctcttggca
     5161 ttgtaaaagc cgctgatgaa atgggctttg aaacaagacc tgttcaagca gataaaactc
     5221 tctttgacat gagtgatgtc ccctatccat ttatcgttca cgttaacaaa gaaggaaaac
     5281 tccaacatta ctatgttgtc tatcaaacaa agaaagacta tctgattatt ggtgatcctg
     5341 atccttctgt aaaaatcacc aaaatgtcaa aagaacgctt tttctctgaa tggactggag
     5401 taactatttt tctagctccc aaacctagct atcaacccca taaagataaa aagaatggtc
     5461 tactaagctt ccttcctctg attttcaaac aaaaatctct tattgcttat attgttctct
     5521 caagcttatt ggtcactatt atcaatatag gtggttctta ttatctccaa ggaatcttgg
     5581 atgaatacat cccaaatcag atgagatcaa ctttaggaat tatctcagtt ggtctggtta
     5641 tcacctatat cctccagcaa gtcatgagct tctcccgtga ttatctccta actgttctga
     5701 gtcagagatt aagcattgat gtgattttat cctatattcg ccatattttt gaacttccta
     5761 tgtctttctt tgcgacacgt cgtacagggg aaattatttc acggttcaca gatgccaact
     5821 ctattataga tgccttggct tctaccattc tttctctttt tctggatgtt tccattctga
     5881 ttcttgtagg gggcgtctta ctggcacaaa accctaatct cttccttctt tctcttattt
     5941 ccattcctat atacatggtc atcatctttt cttttatgaa gccttttgaa aaaatgaacc
     6001 atgatgtcat gcaaagtaat tctatggtta gttctgccat tatcgaagat atcaacggga
     6061 ttgaaactat aaagtcgctc acgagtgaag aaaatcgcta tcaaaatata gacagcgaat
     6121 ttgtagatta tttggaaaaa tcctttaagc tcagtaaata ttctatttta caaacgagtt
     6181 taaagcaggg aacaaaatta gttctgaata tccttatcct atggtttggc gctcaattag
     6241 tcatgtcgag taaaatttct atcggtcagc tgattacctt taacacactt ctttcttact
     6301 ttacaactcc tatggaaaat attatcaatc tccaaaccaa actccaatct gcgaaagtcg
     6361 ctaataaccg tttgaacgaa gtctatctag tcgaatctga atttcaagtt caagaaaacc
     6421 ctgttcattc acattttttg atgggcgata ttgaatttga tgacctttct tataagtatg
     6481 gttttggacg agatacctta acagatatta atctcacgat taaacaagga gataaggtta
     6541 gcctagttgg agtcagtggt tctggtaaaa caactttagc caaaatgatt gtcaatttct
     6601 ttgaacccta caaaggacat atttccatca atcatcagga tattaaaaac attgataaga
     6661 aagtcttgcg ccgtcatatt aattacctac cccaacaagc ctatatcttt aatggctcta
     6721 tcttggaaaa tttaaccttg ggcggtaatc atatgattag ccaagaagat attctaagag
     6781 cttgtgaatt agctgaaatc cgtcaagaca ttgaaagaat gcctatgggc tatcaaactc
     6841 agctctctga tggagctggt ctatcaggag gacaaaagca acgaatcgct ctcgctcgtg
     6901 ctcttttaac taaagctcct gttctaatac tagatgaagc tactagcggt cttgatgtct
     6961 tgactgagaa aaaagttata gataatctta tgtccctcac tgataaaacc attctctttg
     7021 tagcccatcg tctcagtata gccgaacgaa ccaaccgtgt cattgttctt gaccagggga
     7081 aaatcattga agttggtagc caccaagagt taatgcaggc gcaaggtttc taccatcatc
     7141 tgttcaataa ataaggagaa tgtcatgaat cctaatcttt ttagaagcgt cgagttttat
     7201 cagagacgtt accataacta tgcgacagtg ttaattatac ctctttcatt actacttact
     7261 ttcatcttga ttttctccct tgttgccaca aaagaaatta ctgtcacttc ccaaggagaa
     7321 atcgccccta caagtgtcat tgcatctatt cagtcaacaa gtgataatcc tatccttgct
     7381 aatcatttag tggcaaatca agtagttgaa aaaggggact tactcatcaa atactctgaa
     7441 acaatggaag aaagtcagaa aactgcctta gaaactcaac tacacagact tgagaagcaa
     7501 aaagaaggac ttggaatttt gaaacaaagc ttagaaaaag cgactgatct tttttctggc
     7561 gaggatgagt ttggctacca taataccttt aggaatttta ctaaacaatc ccatgatatt
     7621 gaactgggta tcacaaagac taacactgag gtttcaaatc aagccaatct tgccaatagc
     7681 agttcatcag ccatcgaaca agaaattaca aaagttcaac aacaaattgg agaatatcaa
     7741 gagctgagag atgctatcat aaataacaga gctcgcttac caactggcaa tccgcaccag
     7801 tcaattttga atcgttatct tgtagcctca caaggacaaa cacagggaac tgcagatgag
     7861 ccatttttat ctcaaattaa tcaaagtatt gcaggtcttg aaacatctat cgcaagcctc
     7921 aaaattcagc aagctggtat cggaagtgta gcaacttatg ataatagttt agtaaccaaa
     7981 attgaagtac tccgcactca gtttttacag acagcctcac agcaacaact aaccgtggag
     8041 aatcaattaa cagaattaaa agtacaacta gatcaagcga cacagcgttt ggaaaacaat
     8101 actttaaccg ctccaagtaa aggtatcatt cacttaaaca gtgaatttga aggtaaaaat
     8161 agaattccaa ctggtacaga aattgctcaa atatttcctg tcatcacaga tacaagagaa
     8221 gtactaatca cttactacgt atcttctgac tatctacctc tactagataa aggacaaact
     8281 gtaagattaa aactggagaa gattggaaat cacggcatta ccatcatcgg ccaacttcag
     8341 acaattgatc aaactcctac cagaacagag caaggaaatc tctttaaatt aaccgctctt
     8401 gcaaaactat ctaacgagga tagtaaactc atccaatatg gcttacaagg tcgcgtcact
     8461 agtgtaactg caaagaaaac atattttgat tatttcaaag ataaaatgtt aacccattct
     8521 gattaatttt cagataacac tctataacta tttattatct tataaaacag gagaatcata
     8581 acatggataa gaaacaaaac ctaacttcat ttcaagaact aacaactacc gaactcaacc
     8641 agattacagg tggaggatgg tgggaagatt tattatatag atttaatcta attgaacaaa
     8701 acaatacaaa aggatttaat caaccaatac aactataaaa aataagaccg agaaacaaaa
     8761 actctcggtc ttatttttca tcattctata ggtatcacag taagcacctg acgaaagact
     8821 tgattttgac aggtggtatt tagactggta ttaggatgac tttcaacaat cttaatgaca
     8881 gtataaagac caactcctct ctcctcccct ttagaacttg ctccaaagga gaagatttca
     8941 gaaagatcga tgacctcctc tttgatggag ttttcaatga taaaggtctc ctgtgctcca
     9001 ttttttaaaa aggcaattga aacatgaggt tgagtagcct ctgcactagc ttcaatggca
     9061 ttatcacaaa ggatagacac aatggttaga aaatcaagca ggctcatccc ctcgacctga
     9121 atctcctcag gaacttcgac attaaagaca atcttcttat ctctggcttt tataaatttc
     9181 cctgctagga gacttttgag ggcacggtca cgaacattta ccaatctgcc aagatcatat
     9241 ttattgtcct gcaatttctg actggaatcc tttaagacgg aatcgtagac ctcttttatc
     9301 tgctccatat cctcctcttc aatacccaga cgtaaactag tcaagaggtt ggtgtagtca
     9361 tggcgaaaac cccgtacttc cttgtaaagt tcctctatat gccgactata gcgttccata
     9421 tctctatagc gcaaggcctg ctcttggttc agtctctcac ggagtttgtc cttcaaatag
     9481 gtatctaact tcttgataat tcccataaaa aagagtaggt aaaagactag gatgaaatgg
     9541 cgaacagtcc ttaattgaat accttgttca tattcaaggt aagatagact ttgcatcact
     9601 agataataag cccccattgc ccagttaatc ttagtaagag acttttgaaa atctttatct
     9661 agaatctccc ttctcaatct agtgaaatca tagtccaacc atttcaaaaa gactaaaact
     9721 atgaagcaaa cgaatatcat aatcaataaa aagataggat tgctatcttt atctacaatt
     9781 tcttgtccca aaaatggaag tacaaaatag gagactcctc tataaaagag attcaccaat
     9841 atcattggaa agagaccata aaagaaaagg agttttttag gaagccctct taacaataag
     9901 aaagacaaac ctatgccgaa caaaggttct attaagtatg agagatagtc agtcaggact
     9961 aaaaatttaa aagttgtaaa aattgctgcc agaagaaatt ttaaaagaaa ggccttaaaa
    10021 attctatcga aagtgagact aattccatct accttaaaga atatgacaat ttttagtcca
    10081 ttaataataa gtgaatacaa caatatccaa gcaatgttca taaactctcc tagctcagtg
    10141 taagttattg atggcctcag acacttccct gaccttataa cgcgctatca tacagcttcc
    10201 accattggga aagaaaagga gtttttcttt cttatccaaa tgcaccacat ttgcaggatt
    10261 gatgagaaaa gagcggtggc actgcaagag acggggctcc tgcttgaaaa cctcctctaa
    10321 actcgctgta aattccagcc tgtccgtctt ggtatagaga ataacacgat gggctctggg
    10381 cgacgtttca agatagtaaa cctctttaaa aggatactgg aattgggcaa attttgattt
    10441 aaagtaaaag caatcttccg ccagactttt actgtcttga ctattggcat agagaagggc
    10501 tgtttcgata cgagattcaa actcctctgc cgaaagagcc ttatcaatgt agtccaaagc
    10561 agacacttgg tagcgaaagg acaggggcat aaactccgag tgagtcgtca caaagacgat
    10621 gagagcataa ggatctcgat cccgaatttt tctagccact tccagcccct tcatctcctc
    10681 atttcgaatc tcaatatcca aaaagaatag ctgatgggct cccttctcat gcacctctga
    10741 cagcagctgg tctggtttgc caaaaacttc aaaagaactg ggagtgatat gatgttcctt
    10801 caagagtttc tcaatcgttg tttcaattct atcttgttgg gaaaaatcat cttctaaaac
    10861 aaatattctc atcttcttac tctccttctt tcaaccattt ttgacgaatt tctctatagc
    10921 gacgtctggc atacttgaca gcgtattgac cttcttctcc aagaaaaagg attctttctt
    10981 gaaaatcaat cgattttact ttatcaagat taaccaaaca atttcgatga catctcatca
    11041 aggtttcccc atattggttc tcaagattac ttaaattttg aaccatatta aaactagctt
    11101 cttctgtaac aatctgtaca gtatgggctt tagttggatg tgtttggata tagtagacat
    11161 cgtcgacgtt tactttatag actgttttct gtgtctgaat aatcatatgt ttcattggat
    11221 tctcctccta agaaaagaat aacagaaaaa atctaacaca agcacaccca atcctaaaca
    11281 ggccttcaac gctcctgaaa ggtagcttgt tcctcaaata agcatgataa tcttaccact
    11341 attttatcat aaaattcact cagcgcaatt caagacattt caatgacaat taaagatttg
    11401 aataaaaaaa aaagagttaa aagtgataaa ctatgattat agaaagatta tgtttgcaat
    11461 aactttaaaa atcatcaaaa aaggagggct gcatatgatt tattcacatc caccatgatt
    11521 aacaccctat aaatatactc tattgcaatt gacgtaaata taacaaggag aaaattatgg
    11581 ccaagtcaaa taaaacaaaa caattaacat caatcgaact ttctcatata aatggcggta
    11641 gtattccata tggatggtat tctccgtttc atataattag cgataatgta ctatgtagaa
    11701 atggatattc atataataaa gctaatcttg ataatggtaa atgtatcatt gattggggca
    11761 acatagttgg caatgttgct aataatatag gtgcctcact aggagattta cacaatccct
    11821 taccttaaaa tataaattat ataggaggtc acgtaaatga cagacccaat caaaacagct
    11881 cagactttga ttacggactt aaacaaagcc taccaagcat gcaaacaggc aacagctgac
    11941 gacgtccgct ttcaggagca attaaactct attcttggtt ttctagccaa ggctgagact
    12001 gtggataatc gcgtcttgat tgaactggaa aaattctacc agacttccag tcttctcatg
    12061 ggactcagcg ccctcaatcc agatgctcct acacgcgccg cttggcgggc ctatgaccgc
    12121 ttccactttg accaagtcaa gaccaagttg agtc
//
