LOCUS       NZ_VFSH01000001        10285 bp    DNA     linear   CON 12-DEC-2024
DEFINITION  Streptococcus shenyangsis strain D19 Scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_VFSH01000001
VERSION     NZ_VFSH01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus shenyangsis
  ORGANISM  Streptococcus shenyangsis
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 311998)
  AUTHORS   Xiao,C., Li,X., Sun,Y. and Liu,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2019) Key Laboratory of Environmental Pollution
            and Microecology of Liaoning Province, Shenyang Medical College, No.
            146, Huanghe North Street, Shenyang, Liaoning 110034, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAP denovo v. 2.04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006385785.1-RS_2024_12_11
            Annotation Date                   :: 12/11/2024 12:53:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,899
            Genes (coding)                    :: 1,820
            CDSs (with protein)               :: 1,820
            Genes (RNA)                       :: 55
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 23 of 79
            Pseudo Genes (incomplete)         :: 50 of 79
            Pseudo Genes (internal stop)      :: 25 of 79
            Pseudo Genes (multiple problems)  :: 17 of 79
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 21:16:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 170298
            Orig. end                         :: 180583
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VFSH01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10285
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10285
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10285
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5285](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5285)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    198..216
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.46"
                     /tool="antismash"
     gene            complement(217..1338)
                     /gene="mnmA"
                     /locus_tag="FJR77_RS00950"
                     /old_locus_tag="FJR77_00950"
     CDS             complement(217..1338)
                     /EC_number="2.8.1.13"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="mnmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /locus_tag="FJR77_RS00950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00950"
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="WP_049543159.1"
                     /transl_table=11
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVMCN
                     KEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLSQEQ
                     LQKTMFPLGHLEKPEVRKLAEEAGLATAKKKDSTGICFIGEKNFKNFLSNYLPAQPGRM
                     MTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQGFYHD
                     SLMSTSLEASQVHFTRDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFAEPQRAI
                     TPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            complement(1495..1710)
                     /locus_tag="FJR77_RS00955"
                     /old_locus_tag="FJR77_00955"
     CDS             complement(1495..1710)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838227.1"
                     /locus_tag="FJR77_RS00955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00955"
                     /product="hypothetical protein"
                     /protein_id="WP_140832857.1"
                     /transl_table=11
                     /translation="MKHFFAGIGEIHLLSLLLYLGMSTSPIIGLVIGHTKINLINSIII
                     ALGMILALILVLSRVYRKYFYRDDDD"
     gene            complement(2212..2841)
                     /locus_tag="FJR77_RS00960"
                     /old_locus_tag="FJR77_00960"
     CDS             complement(2212..2841)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593110.1"
                     /locus_tag="FJR77_RS00960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00960"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_140832855.1"
                     /transl_table=11
                     /translation="MIELKNITKTIGGKVILDHLSLRIDQGDLVAIVGKSGSGKSTLLN
                     LLGLIDGDYSGQYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLMLAL
                     KYVKFSKKDKLKKVEEILERVGLPATLHQKVSELSGGEQQRIAVARAILKPSQLILADE
                     PTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIEL"
     gene            complement(2838..4946)
                     /locus_tag="FJR77_RS00965"
                     /old_locus_tag="FJR77_00965"
     CDS             complement(2838..4946)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /locus_tag="FJR77_RS00965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00965"
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_140832853.1"
                     /transl_table=11
                     /translation="MKKISHLCMFLLLLCTTFFVFNVNYTREAVRIREMGKTVDSLDLY
                     LKDINKPAASVLRFFEDVSKEYKVSIIKTDSGDEVIKSGVFDKDTFPYQEFGISSLDFT
                     TDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEMDKD
                     RIVQKWSDFFKIDQAALLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQPMMEM
                     KRVGVQKLLGFQSRAILAGFVKTNFYLLLGGSLVIDLGLFLVLDYRPKLLFPSLLLSQF
                     LLLQLYLFISWLTYLMIQKMTVSSMLKGFSSVKLGLIFNYVMKIGTTILLTALLIGVGR
                     SLEQENKELAYQQQWVSQGNYLTLETFQLNDNLWQEELAGSGKSIDYFYQFYQDLLAKI
                     QVNYVRSASLPIKPVIKAEQVQQYQLPDKVDVYYANSNFLKSKGFKLPDTSTKKVILMP
                     ASDRGQEGKNQFLGKSIAYLSMRYEEQQKQRIEDMDVEIAYYEGDWSFFPYNNERKENL
                     HNPIISLVNDQDMMWEEKTRLSTTGLNNPMKVENTEQNQKVITELVEKLLDGNYLKFSS
                     IQAIQQEKVDSYRDAVRNFNILFALFGLLSMMISYFLLVTTFLLKRTDIVTKKFMGWKL
                     VDRYRPLLVLLLLGYSLPLLVLIFFAHALLPLLLFAGFTCLDILFVLVLASRMEKRSLA
                     ELLKGGIL"
     gene            complement(5001..5285)
                     /locus_tag="FJR77_RS00970"
                     /old_locus_tag="FJR77_00970"
     CDS             complement(5001..5285)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230167.1"
                     /locus_tag="FJR77_RS00970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00970"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_125451172.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 2.9e-28,
                     bitscore: 92.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVDGGQWNYGVGWTGTFGY
                     SDYLHSTRSHTATVKHGGRISKDREDPEVWAQASLNKIPPTGLEYFYGF"
     misc_feature    5331..5347
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 23.57"
                     /tool="antismash"
     gene            5668..6525
                     /locus_tag="FJR77_RS00975"
                     /old_locus_tag="FJR77_00975"
     CDS             5668..6525
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /locus_tag="FJR77_RS00975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00975"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_140832851.1"
                     /transl_table=11
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPKY
                     ENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQNYL
                     KTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLKILN
                     TILFSLPIEHLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNICQQIC
                     DTLLEDAKKKKKYDSLAVAYVRIGICRDDAKLIQKGFSLLELVDETSMLSHLKQEVETY
                     YQAK"
     gene            complement(6583..7203)
                     /locus_tag="FJR77_RS00980"
                     /old_locus_tag="FJR77_00980"
     CDS             complement(6583..7203)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /locus_tag="FJR77_RS00980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00980"
                     /product="LysM domain-containing protein"
                     /protein_id="WP_140832849.1"
                     /transl_table=11
                     /translation="MKSTTKKIKTTLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLSE
                     IAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVAPASTTYAAPAAQDETVSAP
                     VVETTEVVEEVPVASEAVAEETVASTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQ
                     LTDSYLNGDYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     misc_feature    complement(7273..7289)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 26.23"
                     /tool="antismash"
     gene            7443..8114
                     /gene="sdaAB"
                     /locus_tag="FJR77_RS00985"
                     /old_locus_tag="FJR77_00985"
     CDS             7443..8114
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /locus_tag="FJR77_RS00985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00985"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="WP_000838912.1"
                     /transl_table=11
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPTE
                     VEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKDSN
                     APHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGM
                     IALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFF
                     K"
     misc_feature    complement(8123..8139)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.79"
                     /tool="antismash"
     gene            8123..8995
                     /gene="sdaAA"
                     /locus_tag="FJR77_RS00990"
                     /old_locus_tag="FJR77_00990"
     CDS             8123..8995
                     /EC_number="4.3.1.17"
                     /GO_function="GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /GO_process="GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="sdaAA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /locus_tag="FJR77_RS00990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00990"
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="WP_140832846.1"
                     /transl_table=11
                     /translation="MFYSIKELVEQANLDFQGNVAELMITTEFELTGREREEVFLLMER
                     NLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNGKTLSDYTILSAARNAIAVNEHN
                     AKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASISGA
                     EGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEVPCVK
                     RNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAATPTGR
                     RLQKEIFGE"
     gene            9017..9649
                     /locus_tag="FJR77_RS00995"
                     /old_locus_tag="FJR77_00995"
     CDS             9017..9649
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /locus_tag="FJR77_RS00995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FJR77_00995"
                     /product="HAD family hydrolase"
                     /protein_id="WP_140832844.1"
                     /sec_met_domain="HAD_2 (E-value: 4.8e-40, bitscore: 131.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTHTFKELGVPSPDTKTIRG
                     FMGPPLESSFATCLPKEQITEAVQIYRSYYRAKGIHEAQLFPNIVDLLEKLSSSYPLYI
                     TTTKNTPTAQDMAKNLGIYHFFDGIYGSSPEAPHKADVIRQALQTHQLSPEQVIIIGDT
                     KFDMQGAQETGIQKLAVTWGFGEQADLLNYQPDYITHKPLEILEYFQ"
ORIGIN
        1 tttgaagaat caaagctgtc gcccgaactc caaaaaccgt attgtctact tttgtccgaa
       61 agtcttgctg agtcattctt atcctttccc ttaaacgata caaaaacagt caaaactcca
      121 aagaagtgca ggacaaaaaa gcctgcaaca tccatgagct ttgaccatca tttctattgc
      181 tgttattatt gtagcagatt gagaaaattt gtcaatctaa atgtactgac aaacttgtcc
      241 atcgcggtaa gcattgtcaa tcaagccacc acctagacac tcctcgccat cgtaaaagac
      301 aactgcctgt cctggtgtaa tcgcacgttg tggctctgca aagatgacct ctgccttgtc
      361 tcctttaaca tgtactgtca ccttagaatc aggctgacgg tagcggaatt tagccgtaca
      421 ttcgagcgta aactcctctg gcatatcacg agtaaagtgg acttggctag cctctaggct
      481 ggttgacatg agcgaatcat ggtagaaacc ttggcctaca tagaggatat tcttgcttag
      541 gtcttttccg acaacgaacc aaggggcatt gtcaccgccg tgttgcccac cgataccgag
      601 tccgccacgc tgaccgatcg tataatacat cagacctgca tgctcgccca tatcgcgacc
      661 atctacagtc atcatgcgac caggctgagc tggtaagtag ttgctgagga aatttttaaa
      721 gttcttttct ccgataaagc aaatccctgt cgagtctttc ttcttagcag tcgcaaggcc
      781 tgcttcttct gctagttttc gaacttcagg cttttccaaa tgtcccagtg gaaacatggt
      841 tttttgaagt tgttcttgcg aaagttgact gaggaaataa gtctgatcct tgccattgtc
      901 cacgccacga agcatgtgaa cagtgccatc ctcatcacgc gctactcgag cataatgccc
      961 agttgctaca tagtctgcac ccaaggtcat ggcatagtcc aaaaaggcct tgaacttgat
     1021 ttccttgttg cacataacat ctggatttgg cgtgcgccct gcacggtatt ccgctaggaa
     1081 atactcgaaa acgcggtccc agtactcttt ttcaaaattg acagagtagt aaggaatgcc
     1141 aatctggtct gctaccgcag ccacatcctt gtaatcttcg gtcgccgtac agacgccgtt
     1201 ttcatctgtg tcatcccagt tcttcatgaa gataccgatc acatcgtagc cctgctcttt
     1261 gagcaagaga gccgtcaccg acgaatcaac accaccactc atccccacaa caacacgtgt
     1321 tttagagtta tcactcatgg taagtctccc atctattcgt ttattcacga ttgaaggtcg
     1381 tgtgtgcttc acgattgaag gtcgcttgag cagttttcat tataacatgc ttggttagag
     1441 aagacaagaa agaggctgat aatctaccaa cctctggttt tattgataaa aactttagtc
     1501 atcatcgtct ctataaaaat attttctata cactcttgat aataccaaaa ttaatgccaa
     1561 tatcatacca agagctataa taatagaatt tattagattt attttagtat gtccaatcac
     1621 tagtccaata attgggctag tagacatgcc tagatataat aaaagtgaca aaagatgaat
     1681 ttcaccaatt cctgcaaaaa aatgtttcat gtaatgcttc tccatttgct ccattaaatc
     1741 tattttattt tagagtagca taaaataagc aatccttcaa taaaatccat tgacgaaata
     1801 ctattctaaa gcattgataa ctcagatttc tcagaataga gctgcccaaa ctatcgaaca
     1861 gctcaagaac atatatatct atgcgattct aaacttagaa ttgctataga ataaatgttt
     1921 tatgggtatt ctaggtcttt gactgcttaa aattttacgt tctgaaggat tctaaagcta
     1981 gaatagcata aaaatcatga aaactgaact attcgaagct tcatagtcat acgatttcca
     2041 taggaagtat ctttttagta cgtgtggtta ggaggtaatt ttagggaatc tcccaaaaac
     2101 aaccaatttc ctatttttat caatcaaaaa atgttgataa atgagtgttt taaaattaaa
     2161 ttttaaaggt atccttaaat ctaaagattt ttgataagat ttctgtattt ttcacaattc
     2221 aatgattcga tgacattgtt gggctacata agcatcgtgg gtcacgataa tgactgtttt
     2281 cccctcgcga ttcatctcta agagaaactt caagaccaaa tctctatttt caggatccag
     2341 agaacctgtt ggttcatcgg ctaaaatcaa ctggctgggt tttaagatgg ctctagcaac
     2401 tgcaattcgt tgttgttcgc caccagacaa ctcggaaacc ttttgatgca aagtagctgg
     2461 caaacctact ctctctaaaa tctcttccac ctttttgagc ttgtctttct tagaaaattt
     2521 cacatatttc agcgccagca tgagattgta ctcgaccgtt tcatcatcaa tcagggcaaa
     2581 attttgaaac agataagaga tatgttcacg gattattgtt tgcgacttag cagaattaac
     2641 cgctagattt gtctgaccaa aaatctcata ctgtccgcta taatcaccat ctatcaaacc
     2701 taataaattt aacaaggtcg acttgccact gccacttttt ccaacgatgg caaccaaatc
     2761 cccctgatca atcctgagag ataagtgatc caaaatcact tttcctccaa tggttttggt
     2821 aatatttttc aactcaatca taagatgccc cctttcaata actctgctag acttcttttc
     2881 tccatcctag aagccaatac tagcacaaat agtatatcca gacatgtaaa gcctgcaaac
     2941 agcaggagtg gcaagagcgc atgggcaaag aaaatcaata ctagaagagg gagactatag
     3001 cccaacaaga gcagaactaa gagagggcgg tagcgatcga ccagtttcca ccccataaac
     3061 ttcttggtaa cgatatctgt tcgcttcaat aagaaagttg ttactaataa gaagtaggaa
     3121 atcatcatgc taaggagacc aaacaaagca aagagtatat taaaatttcg aactgcatct
     3181 cgataagaat ctactttctc ttgttgaatg gcttgaatcg atgaaaattt taaataattc
     3241 ccatctaata atttctcaac taactccgta atcacttttt gattttgttc tgtattttca
     3301 actttcatcg gattgtttaa acctgtcgtt gacaagcgag tcttttcttc ccacatcatg
     3361 tcttgatcat ttacaagact aatgattgga ttgtggaggt tttcctttcg ctcattatta
     3421 taagggaaga aagaccaatc tccttcatag taggcaattt ctacatccat atcttctatc
     3481 ctttgctttt gctgttcttc atacctcata gaaagatagg cgatggattt ccccaagaac
     3541 tgattcttgc cttcttgtcc tctatcactg gctggcatca aaataacttt tttagtgctg
     3601 gtatctggta acttgaatcc cttactcttt agaaaattgc tattagcata gtaaacatct
     3661 accttatcag gcagttggta ctgctgcact tgttctgctt tgatgactgg tttgatagga
     3721 agactcgcac ttcgaacata atttacttgt atttttgcta gtaaatcttg ataaaattgg
     3781 tagaaataat ctatagattt ccctgaccct gctagctctt cttgccacag gttatcattg
     3841 agttggaagg tttctaaggt caagtaattc ccttggctta cccactgttg ctgataagca
     3901 agttctttgt tttcttgttc tagacttctg cccaccccaa tcagtaaggc cgtcagtaaa
     3961 atagttgtcc ctattttcat cacataattg aagataagac caagtttgac agatgaaaaa
     4021 cctttcaaca tggaactgac tgtcattttc tgaatcatca ggtaggtcag ccaactgatg
     4081 aacaaataca actgtaaaag cagaaattgg gataagagta aactcgggaa caaaagtttt
     4141 ggtctgtaat ccagcactaa aaataatccc aagtcaatga caagacttcc acccaagaga
     4201 agataaaaat tagtctttac aaaaccagct aaaatcgctc tgctttgaaa accaagtaac
     4261 ttttgaactc ccactcgttt catctccatc atcggctgat aaactgttac taacacaaga
     4321 agtaaaatag ccaagacaaa aacaatggca gataaaagca aatctcgatt tatgacttcc
     4381 actgcacttt tataggtagg ctctagcaag gcagcctggt ctatcttgaa aaaatcgctc
     4441 catttctgta caatcctatc cttatccatc tcttgtgtag aagttatcgt atagcgacca
     4501 tttaaactac gagatgtatc cttgatatag gtttgaaaag tcataagctg aataggcttg
     4561 gcttttagaa aggtcggaat cgtaccaagt ttattagaaa tttctttatt actgtagact
     4621 ccttcaccat ctgtggtaaa atcaagagaa gaaatcccaa actcttggta ggggaaggta
     4681 tctttatcaa aaacaccaga cttgattacc tcatcaccac tgtctgtttt gatgatagag
     4741 accttgtact cctttgatac atcctcaaaa aatcgaagaa cagacgctgc aggtttgtta
     4801 atatctttca aatacaaatc caaagaatct acagtctttc ccatttcccg aatccgaacc
     4861 gcttcacgtg tgtagtttac attaaaaaca aaaaaagtgg tacacagcaa cagcagaaac
     4921 atacaaagat gactgatttt tttcataaaa gtacctccac taaagttctc cctctcctaa
     4981 taggagaggg aaaatttcat ttaaaatcca tagaaatatt ctaatcctgt tggcggaatt
     5041 ttattaaggg aagcttgagc ccaaacctcg ggatcttcac gatccttaga gattctacct
     5101 ccatgcttga cagttgctgt atgagatcga gtagaatgta aataatcaga atatccaaaa
     5161 gttcctgtcc aacctactcc atagttccat tgaccaccat caacccatac tgctaaagca
     5221 tttgtagtag caagtatacc acataccatt aaagatgcaa caccaatttt aactaatttt
     5281 ctcatgattt atttctccta ttatcgagag ctgttccgta aaaacagcta tagtaattta
     5341 tcaacaagta tcgtagtcca ttggactcac cttcctttca tttttacaac tagcacatta
     5401 tatcggccat tgccatggga aaaccgtagc aatgctgaat aagaaagcaa gaataactaa
     5461 aaagcgaaaa acttttttat tcatactctt tccctcctat ctgtattttg tatctatagt
     5521 atacctcttc tttactctat tttaaaatta gttaactttt ttcacacatt attttagttt
     5581 tcttttttac tttttacatt gctatgatac aatattttta acaaaatttt attgtttgtt
     5641 aacttttttc acgaaaagga gaaaatcatg cgttatgatt tcggaaaggt ctataaagaa
     5701 atacgtgagt caaaggggct gacccaagaa gaggtctgtg ggggtgttct ctcaagaacc
     5761 agcctatcaa agattgaaag tggcaagaca accccaaaat atgaaaatat ggaatttctt
     5821 ctccgtcaaa tcaatatgag ttttgaagag tttgaatata tctgtcaact ttatcaacca
     5881 agtcaacgca cagagattat gcaaacctat ctcaatatgc gttccattat cgggaccagc
     5941 gatcttgtca atttatttca aaaatgccaa aactatctca agacccacca cgatctccct
     6001 atagaagaaa tcagagacat gcttgaagtt gttatttata tccgtcaaca cggggcaggg
     6061 gaactatcag atcacgcaga acaggttgtc aaaaaacttt ggagaaagat tgaaaaacaa
     6121 gatacatggt atgaaagtga cctaaaaatc ctcaatacca tccttttcag cttgcccatt
     6181 gaacacctcc atctcatcac tggaaaaatc ttgcaacgct tagaagtcta taaaaactat
     6241 caacatttat atgacttgcg aatgacaatt ctattaaacc tttccacact ctatctatac
     6301 aatcaagata aaaacatatg ccaacaaatc tgcgatactt tactagagga tgccaagaaa
     6361 aagaaaaaat atgatagctt ggctgtcgcc tatgtccgta tcggaatttg tagggatgat
     6421 gctaaattga ttcaaaaagg tttctcactt ctggagctgg tcgatgaaac ttctatgctg
     6481 tctcatctca aacaagaagt ggagacctat tatcaagcga agtaaagata aaaaagtcga
     6541 gggagttcct cgaccttttc atattattct gttcgttatt tcttaatacc agccgttgtt
     6601 aagccagaag tttttagcgg ctgtccatga accgtaacgt cctgcaacgt aggcatctgc
     6661 tacacgttct tggttttcag ctgagtagtc accgttcaag tatgaatctg tcaattggta
     6721 acgtccgatg tagcgtccgt ttgtagctgt gtagctaccg cctgattctt tttgagcgat
     6781 ccattcttta gcttctgctt cagatccgct tacagttgaa gcaaccgttt cttctgctac
     6841 tgcttcgctt gctactggaa cttcttccac tacttctgta gtttctacta ctggagctga
     6901 aacagtttca tcttgagcag ctggtgctgc ataagtcgtt gaggctggtg ctactggcgc
     6961 tacaggacca tcgataacca actcttgacc aacataaatc aaatgaatgt tgtcaatgtg
     7021 gttgttttct gccaatttct caacagttgt attgtgagtt tcagcgattt ctgaaagtgt
     7081 atcaccttct ttaactgtgt aagttgatga ttcttgagca gatacaaatg atggagcaaa
     7141 tactgcaaac aaggcagcta ctcctgcaag agttgtttta atctttttag ttgttgattt
     7201 catatttgaa aattctcctt ctttctatac ttctatcata ccctttaaat attaccgttt
     7261 cttgacactt ttatgtagaa atattacaaa attatttttt atttccctaa ataagctatt
     7321 ttttgttaca aaagatgctt ttttatgcta tttgtagtgt aaaaaggttt tcatccacaa
     7381 ttaaagccaa gacctttatt ctttgatata atagagataa gaatttttat aaggggaaac
     7441 tgatgaaatc acttcgtttt caatctgtct ttgatatcat cggaccagtt atgattggcc
     7501 catctagtag tcataccgct ggggctgttc gtattgggaa gattgtctct tccatttttg
     7561 atgatactcc gacagaagtc gaattccaac tttttaactc atttgccaag acctatcgtg
     7621 gtcacgggac agacctagcc cttgttgctg gtattttagg catggataca gatgatcctg
     7681 aaatcccaaa cagtctggag attgcccaca agcgtggtat caagattgtc tggaccattc
     7741 agaaagacag caatgctcct catccaaaca ccactaaaat taccgttaaa aatgcccaca
     7801 agactatcag tgtgactggt atttctatcg ggggagggaa tattcaggtc accgaactca
     7861 atggttttgc cgtctctctc aatatgaata caccgactat catcatcgtt catcaagata
     7921 ttccaggtat gattgccctc gtaacagagg cactttcccg ctatggtatt aatatcgccc
     7981 agatgaatgt tactcgtgaa aaagctggtg aaaaagccat tatgatcatc gaagtcgaca
     8041 gtcgcaactg tgatgaggcc atcgaagaaa ttcgaaaaat ccctcatctc cacaatgtca
     8101 atttctttaa ataggaggaa gcatgtttta ttctatcaaa gaattggtcg agcaagcaaa
     8161 tctggacttt caaggaaatg tcgcagaact catgattaca acagagtttg aattgacagg
     8221 tcgcgaacgt gaagaagtct tccttctcat ggaacgcaat ctggaagtca tgaaagcctc
     8281 tgtccaactt ggactcaatg aaaataaatc tcgtagtggc ctgacaggtg gagatgctgc
     8341 caaactggat cactacatca aaaacggaaa aactttatca gattacacga ttctctctgc
     8401 tgcccgaaat gctatcgcag tcaatgaaca caatgctaaa atgggcttgg tctgtgccac
     8461 tccgaccgca ggaagtgctg gctgtctccc ttccgttctc actgctgcta ttgaaaaatt
     8521 agaccttagc cacgagcaac agctagattt cctctttgct gctggtgcct ttggactagt
     8581 catcgcaaat aatgcctcta tctcaggtgc tgagggcggt tgccaagccg aagtcggttc
     8641 ggcctctgct atgagtgctg cagccttgac tctggctgca ggtgggacac cctaccaagc
     8701 cagtcaagct attgcctttg tcattaaaaa tatgctaggc ctcatctgtg accctgttgc
     8761 aggtttggtt gaagttccct gtgtcaaacg taatgccatg ggagctagct ttgccttcat
     8821 cgcagcagac atggccctgg caggtatcga atctaaaatc cctgtggatg aagttatcga
     8881 tgccatgtac caagtaggag caagcatgcc gactgccttt cgtgaaacag ctgaaggtgg
     8941 actcgctgcc acccctactg gtcgtcgcct ccaaaaagaa attttcggag aataagctta
     9001 tcaaatagga gaaaccatga cctctatcac agctatcttt ttcgatctag atggaaccct
     9061 cgttgacagt tctatcggga ttcacaatgc ctttacccat acctttaaag agctaggagt
     9121 gcctagtcct gataccaaaa cgattcgtgg ttttatggga ccgcccctcg aaagtagttt
     9181 tgcgacctgc ctacccaaag aacaaatcac tgaggctgtg cagatatacc gttcttacta
     9241 tagggcaaaa ggcatccatg aagctcaact ctttcctaat attgtagact tgcttgagaa
     9301 gttatcgagc agttatccac tctacatcac cacgacaaaa aatactccaa ccgctcaaga
     9361 catggctaaa aatttgggaa tctatcattt ctttgatggc atttatggtt ccagccctga
     9421 agctccccat aaggccgatg tcattcgcca agccttgcag acacatcaac taagccctga
     9481 acaagtcatc atcatcggag ataccaagtt tgatatgcag ggagctcaag aaacaggcat
     9541 tcagaaattg gccgtcactt ggggatttgg agagcaagca gatctactaa actatcaacc
     9601 tgattatatc acccacaaac ccttagaaat tttggagtat tttcaataac atagactgag
     9661 cgacaacccc atttcagaag ataatgaagt aaatctctac ataacaaaac gcatattacc
     9721 aaggttcaag atgtactgca ccccaaaaag ttagattttt tctgtctaac ttttggggtg
     9781 cagttcaatt caaggactga taatatgcgt ttttcttttt tattcaacgt gggttgtttg
     9841 atttgcaaag gcgataatag cttgtttcaa ctcatctcct ctgagagttc ggaactcttc
     9901 aatcttttga tcgatggctt ctagaggcat gacattaacc ttaccaacga aaatcttatc
     9961 cataacttgg ttatcaacaa gttcggttga taccaagagt tcttcgtaga gtttttcacc
    10021 tggacggatt ccaacttcaa cgattggaat ttcgctctca gtgtgcccac ttagaaggac
    10081 cattttcttg gctaagtcat aaattttgac tggtttgccc atatcaagga taaagacttc
    10141 tccatctttg gcataagcac cagcatggat aaccaaacgg ctagcttctg gaatagtcat
    10201 aaagtaacgg gtcatgcgga agtctgtcac cgttacaggc ccaccttcag caatctgacg
    10261 ttcaaagact ggtatgacac tacca
//
