GCF_005864065.1 - 35 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_VANP01000001.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	RRE-containing	200,305	221,414
Region 1.2	RiPP-like	261,994	272,263
Region 1.3	NI-siderophore,NRPS,T1PKS	761,642	852,174	Medium	FW0622	other:other

NZ_VANP01000010.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 2.1	PKS-like,NRPS-like	296,352	318,508	Low	naphthyridinomycin	NRPS:Type I

NZ_VANP01000012.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 4.1	T1PKS,NRPS	1	158,061	Low	cyphomycin	PKS

NZ_VANP01000014.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	T1PKS,PKS-like	24,942	119,917	Medium	abyssomicin M/abyssomicin N/abyssomicin O/abyssomicin P/abyssomicin Q/abyssomicin R/abyssomicin S/abyssomicin T/abyssomicin U/abyssomicin V/abyssomicin W/abyssomicin X	PKS
Region 6.2	NRPS	172,133	207,844	Low	naphthyridinomycin	NRPS:Type I

NZ_VANP01000015.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 7.1	NI-siderophore	29,672	61,007

NZ_VANP01000016.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 8.1	terpene-precursor	59,322	80,389

NZ_VANP01000017.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 9.1	NRPS	124,013	159,191	Low	marinacarboline A/marinacarboline B/marinacarboline C/marinacarboline D	other:other

NZ_VANP01000018.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 10.1	T1PKS	1	65,339	Medium	salinilactam	PKS
Region 10.2	redox-cofactor	67,442	90,290

NZ_VANP01000019.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 11.1	terpene	21,940	42,908

NZ_VANP01000002.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	lanthipeptide-class-ii	2,919	25,795
Region 12.2	terpene	609,091	630,011	Low	isorenieratene	terpene

NZ_VANP01000025.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 18.1	NRPS,NRPS-like,T1PKS,terpene-precursor	1	61,080	Low	aurachin C/aurachin D/aurachin SS	PKS+terpene

NZ_VANP01000028.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 21.1	T1PKS	1	23,059

NZ_VANP01000032.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 26.1	T1PKS	1	6,013

NZ_VANP01000037.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 31.1	NRPS	1	2,973

NZ_VANP01000004.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 34.1	T1PKS	1	25,336
Region 34.2	terpene	437,122	476,423	Low	hopene	terpene
Region 34.3	azole-containing-RiPP,thioamitides	583,011	614,778

NZ_VANP01000005.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 43.1	T3PKS	107,805	148,875
Region 43.2	lanthipeptide-class-iv,NRPS	157,507	228,799	Low	labyrinthopeptin A2/labyrinthopeptin A1/labyrinthopeptin A3	ribosomal:RiPP:Lanthipeptide
Region 43.3	T3PKS,lanthipeptide-class-iii	446,707	487,786	High	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone/2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	PKS:Type III

NZ_VANP01000006.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 44.1	phenazine,NRPS-like,T1PKS	166,989	229,478	Medium	phenazine SA/phenazine SB/phenazine SC	other:other

NZ_VANP01000007.1 (Microbispora fusca)

Region	Type	From	To
Region 45.1	NRPS-like	110,104	152,860
Region 45.2	lanthipeptide-class-i	195,762	222,187
Region 45.3	T1PKS	278,291	326,162

NZ_VANP01000008.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 46.1	t3nrps-iterative,NRPS-like,butyrolactone,PKS-like,blactam	180,050	260,915	Low	tabtoxin	other:other
Region 46.2	RiPP-like	267,364	278,509
Region 46.3	terpene	288,710	311,180

NZ_VANP01000009.1 (Microbispora fusca)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 47.1	blactam	71,483	93,312
Region 47.2	terpene	141,771	166,699	High	geosmin	terpene:Sesquiterpene
Region 47.3	NAPAA	179,415	213,473

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1.1	RRE-containing	200,305	221,414
Region 1.2	RiPP-like	261,994	272,263
Region 1.3	NI-siderophore,NRPS,T1PKS	761,642	852,174	Medium	FW0622	other:other
Region 2.1	PKS-like,NRPS-like	296,352	318,508	Low	naphthyridinomycin	NRPS:Type I
Region 4.1	T1PKS,NRPS	1	158,061	Low	cyphomycin	PKS
Region 6.1	T1PKS,PKS-like	24,942	119,917	Medium	abyssomicin M/abyssomicin N/abyssomicin O/abyssomicin P/abyssomicin Q/abyssomicin R/abyssomicin S/abyssomicin T/abyssomicin U/abyssomicin V/abyssomicin W/abyssomicin X	PKS
Region 6.2	NRPS	172,133	207,844	Low	naphthyridinomycin	NRPS:Type I
Region 7.1	NI-siderophore	29,672	61,007
Region 8.1	terpene-precursor	59,322	80,389
Region 9.1	NRPS	124,013	159,191	Low	marinacarboline A/marinacarboline B/marinacarboline C/marinacarboline D	other:other
Region 10.1	T1PKS	1	65,339	Medium	salinilactam	PKS
Region 10.2	redox-cofactor	67,442	90,290
Region 11.1	terpene	21,940	42,908
Region 12.1	lanthipeptide-class-ii	2,919	25,795
Region 12.2	terpene	609,091	630,011	Low	isorenieratene	terpene
Region 18.1	NRPS,NRPS-like,T1PKS,terpene-precursor	1	61,080	Low	aurachin C/aurachin D/aurachin SS	PKS+terpene
Region 21.1	T1PKS	1	23,059
Region 26.1	T1PKS	1	6,013
Region 31.1	NRPS	1	2,973
Region 34.1	T1PKS	1	25,336
Region 34.2	terpene	437,122	476,423	Low	hopene	terpene
Region 34.3	azole-containing-RiPP,thioamitides	583,011	614,778
Region 43.1	T3PKS	107,805	148,875
Region 43.2	lanthipeptide-class-iv,NRPS	157,507	228,799	Low	labyrinthopeptin A2/labyrinthopeptin A1/labyrinthopeptin A3	ribosomal:RiPP:Lanthipeptide
Region 43.3	T3PKS,lanthipeptide-class-iii	446,707	487,786	High	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone/2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	PKS:Type III
Region 44.1	phenazine,NRPS-like,T1PKS	166,989	229,478	Medium	phenazine SA/phenazine SB/phenazine SC	other:other
Region 45.1	NRPS-like	110,104	152,860
Region 45.2	lanthipeptide-class-i	195,762	222,187
Region 45.3	T1PKS	278,291	326,162
Region 46.1	t3nrps-iterative,NRPS-like,butyrolactone,PKS-like,blactam	180,050	260,915	Low	tabtoxin	other:other
Region 46.2	RiPP-like	267,364	278,509
Region 46.3	terpene	288,710	311,180
Region 47.1	blactam	71,483	93,312
Region 47.2	terpene	141,771	166,699	High	geosmin	terpene:Sesquiterpene
Region 47.3	NAPAA	179,415	213,473

No secondary metabolite regions were found in these records:: NZ_VANP01000011.1 (Microbispora fusca); NZ_VANP01000013.1 (Microbispora fusca); NZ_VANP01000020.1 (Microbispora fusca); NZ_VANP01000021.1 (Microbispora fusca); NZ_VANP01000022.1 (Microbispora fusca); NZ_VANP01000023.1 (Microbispora fusca); NZ_VANP01000024.1 (Microbispora fusca); NZ_VANP01000026.1 (Microbispora fusca); NZ_VANP01000027.1 (Microbispora fusca); NZ_VANP01000029.1 (Microbispora fusca); NZ_VANP01000003.1 (Microbispora fusca); NZ_VANP01000030.1 (Microbispora fusca); NZ_VANP01000031.1 (Microbispora fusca); NZ_VANP01000033.1 (Microbispora fusca); NZ_VANP01000034.1 (Microbispora fusca); NZ_VANP01000035.1 (Microbispora fusca); NZ_VANP01000036.1 (Microbispora fusca); NZ_VANP01000038.1 (Microbispora fusca); NZ_VANP01000039.1 (Microbispora fusca); NZ_VANP01000040.1 (Microbispora fusca); NZ_VANP01000041.1 (Microbispora fusca); NZ_VANP01000042.1 (Microbispora fusca); NZ_VANP01000043.1 (Microbispora fusca); NZ_VANP01000044.1 (Microbispora fusca); NZ_VANP01000045.1 (Microbispora fusca); NZ_VANP01000046.1 (Microbispora fusca); NZ_VANP01000047.1 (Microbispora fusca)

Compact view

NZ_VANP01000001.1 - Region 1 - RRE-containing

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Location: 200,305 - 221,414 nt. (total: 21,110 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

FED44_RS01030: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002317.2	0.45	ribosomal	herbicolin O, mcceh252, pantocin A	Pantoea agglomerans
BGC0000585.5	0.44	ribosomal	microcin C7	Escherichia coli
BGC0001633.3	0.36	other (other)	ketomemicin B3, ketomemicin B4	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000812.3	0.35	other (other)	huperzine A	Shiraia sp. slf14
BGC0000041.3	0.33	PKS	oronofacic acid	Pseudomonas syringae
BGC0001858.2	0.32	PKS	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0000353.5	0.31	NRPS	tacrolimus	Streptomyces sp. MA6548
BGC0000925.5	0.29	other (other)	quinolobactin	Pseudomonas fluorescens
BGC0001913.3	0.29	PKS	phomoidride	fungal sp. ATCC 74256
BGC0002026.3	0.28	NRPS, PKS	thaxteramide A1, thaxteramide A2, thaxteramide B1, thaxteramide B2	Jahnella sp. MSr9139

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002317.2	0.45	ribosomal	herbicolin O, mcceh252, pantocin A	Pantoea agglomerans
BGC0000585.5	0.44	ribosomal	microcin C7	Escherichia coli
BGC0001633.3	0.36	other (other)	ketomemicin B3, ketomemicin B4	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000812.3	0.35	other (other)	huperzine A	Shiraia sp. slf14
BGC0000041.3	0.33	PKS	oronofacic acid	Pseudomonas syringae
BGC0001858.2	0.32	PKS	alternapyrone B, alternapyrone C, alternapyrone D, alternapyrone E, alternapyrone F	Parastagonospora nodorum SN15
BGC0000353.5	0.31	NRPS	tacrolimus	Streptomyces sp. MA6548
BGC0000925.5	0.29	other (other)	quinolobactin	Pseudomonas fluorescens
BGC0001913.3	0.29	PKS	phomoidride	fungal sp. ATCC 74256
BGC0002026.3	0.28	NRPS, PKS	thaxteramide A1, thaxteramide A2, thaxteramide B1, thaxteramide B2	Jahnella sp. MSr9139

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (9):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.55 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.75 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.27 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.08 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.83 out of 27.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.35 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 21.04 out of 35.63

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.12 out of 32.93

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 19.92 out of 30.98

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Pantocin_Microcin_RRE; Location: 0 - 94; Score: 62.1

TFBS definitions

Regulator	Description

NZ_VANP01000001.1 - Region 2 - RiPP-like

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Location: 261,994 - 272,263 nt. (total: 10,270 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

FED44_RS01340: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Similar gene clusters

Analysis type:

Reference	RiPP-like	Similarity score	Type	Compound(s)	Organism
BGC0000883.5		0.23	other (other)	arginomycin	Streptomyces arginensis
BGC0001652.5		0.18	NRPS	limazepine C, limazepine D, limazepine E, limazepine F, limazepine A	Streptomyces sp. ICBB 8177

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0000883.5		0.23	other (other)	arginomycin	Streptomyces arginensis
BGC0001652.5		0.18	NRPS	limazepine C, limazepine D, limazepine E, limazepine F, limazepine A	Streptomyces sp. ICBB 8177

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.31 out of 18.46

Confidence: strong

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 22.67 out of 26.33

Confidence: medium

Additional weak hits (5):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.81 out of 30.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.16 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.42 out of 36.54

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.84 out of 26.33

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.88 out of 35.63

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
RpoN	Regulator of nitrogen assimilation and virulence

NZ_VANP01000001.1 - Region 3 - NI-siderophore,NRPS,T1PKS

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Location: 761,642 - 852,174 nt. (total: 90,533 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

FED44_RS03780: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

FED44_RS03835: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

FED44_RS03915: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: PPTase acylase methyltransferase

FED44_RS03890: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

FED44_RS03905: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

FED44_RS03970: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: protease

FED44_RS03755: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: decarboxylase

FED44_RS03785: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: ligase synthase

FED44_RS03900: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

FED44_RS03910: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.93	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.92	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.92	PKS	asperlactone	Aspergillus ochraceus
BGC0002075.3	0.85	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001873.3	0.80	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001478.5	0.82	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.77	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0002305.2	0.74	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001453.5	0.73	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000940.5	0.72	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001873.3	0.72	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.74 out of 20.97

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.57 out of 36.53

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 28.57 out of 36.53

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.57 out of 24.78

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (26):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.35 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.31 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.41 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.01 out of 26.06

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.16 out of 25.88

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.03 out of 26.85

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.91 out of 21.55

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 18.94 out of 27.55

Confidence: weak

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.67 out of 29.72

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.68 out of 29.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.71 out of 30.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.48 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.65 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.55 out of 26.06

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.31 out of 20.97

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.19 out of 20.97

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.14 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.66 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.26 out of 24.78

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.58 out of 32.93

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.07 out of 27.04

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.92 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.73 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.19 out of 29.65

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.86 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.00 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 761641 - 852174:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (Me-redmal) + (Ser - Pro)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 789678 - 836839:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (Me-redmal) + (Ser - Pro)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 6, location 797080 - 852174:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (Me-redmal) + (Ser - Pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS03900: Ser - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (453..859): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFCSGEVNLYGPTEATVDVSF
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1516..1918): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVSFQESFMVSSGEENQYGPTESHVVTSF
Nearest Stachelhaus code(s):: Pro DVQFVAQVVK (85% 8Å match)
Stachelhaus code match:: 90% (moderate)

FED44_RS03905: mmal

PKS_AT (536..841): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 150.9
Ethylmalonyl-CoA: 117.0
Methoxymalonyl-CoA: 115.9
Malonyl-CoA: 92.1
Isobutyryl-CoA: 74.6
Propionyl-CoA: 72.0
Benzoyl-CoA: 69.8
2-Methylbutyryl-CoA: 66.7
trans-1,2-CPDA: 61.2
CHC-CoA: 58.1
3-Methylbutyryl-CoA: 51.3
Acetyl-CoA: 46.1
inactive: 27.9
fatty_acid: 19.6

FED44_RS03910: mal

PKS_AT (1676..1967): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 166.5
inactive: 106.3
Methoxymalonyl-CoA: 61.3
Methylmalonyl-CoA: 58.4
Isobutyryl-CoA: 49.6
Propionyl-CoA: 39.9
Ethylmalonyl-CoA: 34.6
Benzoyl-CoA: 32.4
2-Methylbutyryl-CoA: 24.6
fatty_acid: 21.5
Acetyl-CoA: 20.0
trans-1,2-CPDA: 15.2
CHC-CoA: 14.9
3-Methylbutyryl-CoA: 12.2

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CcpA	Catabolite control protein A
DmdR1	Iron(II)-dependent repressor
LexA variant 2	Repressor of DNA damage response

NZ_VANP01000010.1 - Region 1 - NRPS-like,PKS-like

Download region GenBank file

Download region SVG

Location: 296,352 - 318,508 nt. (total: 22,157 nt) Show pHMM detection rules used

Region on contig edge.

PKS-like: (t2pks2 or ksIII)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase oxygenase

FED44_RS25365: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

FED44_RS25395: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: methyltransferase

FED44_RS25400: methyltransferase

smcogs: SMCOG1248: methyltransferase

Isomerases: isomerase

FED44_RS25375: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

FED44_RS25425: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

FED44_RS25430: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.57	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.56	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.56	NRPS	enniatin	Fusarium equiseti
BGC0000307.4	0.55	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.54	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.54	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.54	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.54	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.54	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.54	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.61	NRPS	enniatin	Fusarium equiseti
BGC0000307.4	0.61	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.61	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.61	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.61	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.61	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.61	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 21.84 out of 21.84

Confidence: strong

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.03 out of 29.72

Confidence: medium

Additional weak hits (8):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.64 out of 28.14

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.70 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.98 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.51 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.60 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.39 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.81 out of 29.65

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
MexT	Global virulence regulator
NtrC	Nitrogen regulatory protein C

NZ_VANP01000012.1 - Region 1 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 158,061 nt. (total: 158,061 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

FED44_RS26580: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

FED44_RS26590: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

FED44_RS26645: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

FED44_RS26710: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

FED44_RS26745: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

FED44_RS26750: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

FED44_RS26770: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

FED44_RS26895: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

FED44_RS26985: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: PPTase acylase transferase

FED44_RS26595: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

FED44_RS26730: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

FED44_RS26735: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

FED44_RS26765: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

FED44_RS37690: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: dehalogenase

FED44_RS26815: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Lyases: decarboxylase hydratase

FED44_RS26760: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

FED44_RS26915: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: ligase synthase

FED44_RS26725: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

FED44_RS26755: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

FED44_RS26925: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

FED44_RS26930: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

FED44_RS26935: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transporter

FED44_RS26630: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	1.15	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001164.3	1.14	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	1.14	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001276.3	1.13	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	1.00	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.89	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001244.3	0.86	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001284.4	0.86	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000041.3	0.81	PKS	oronofacic acid	Pseudomonas syringae
BGC0000067.5	0.77	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.73	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.73	PKS	asperlactone	Aspergillus ochraceus
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001164.3	0.66	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.65	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000041.3	0.65	PKS	oronofacic acid	Pseudomonas syringae
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 20.47 out of 20.47

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.63 out of 23.79

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.79 out of 20.97

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.26 out of 19.26

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.73 out of 20.97

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.79 out of 21.84

Confidence: medium

Additional weak hits (51):

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 18.88 out of 27.55

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.10 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.48 out of 27.78

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.37 out of 26.12

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.13 out of 30.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.21 out of 26.06

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.74 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.11 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.87 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.91 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.75 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.44 out of 27.78

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.72 out of 30.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.29 out of 29.38

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.37 out of 30.22

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.76 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.69 out of 26.06

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.34 out of 25.88

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.07 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.72 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.23 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.38 out of 29.38

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.61 out of 28.14

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.68 out of 39.62

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.16 out of 30.22

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.91 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.37 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.90 out of 29.38

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.25 out of 20.97

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.24 out of 29.25

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.53 out of 26.85

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.75 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.68 out of 27.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.40 out of 27.18

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.99 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.80 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.85 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.70 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.44 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 19.65 out of 22.40

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.11 out of 29.65

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 35.63

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.96 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.57 out of 29.65

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.99 out of 32.93

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 20.94 out of 32.93

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 158061:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal) + (ccmal) + (emal - Me-redmal) + (Me-ccmal - Me-ohmal) + (Me-ccmal - Me-ccmal) + (Me-ccmal) + (pk - NMe-Ser) + (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 26541:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 19342 - 108092:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (emal - Me-redmal) + (Me-ccmal - Me-ohmal) + (Me-ccmal - Me-ccmal) + (Me-ccmal) + (pk - NMe-Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 105269 - 158061:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS37690: pk

PKS_AT (567..864): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 225.8
Methylmalonyl-CoA: 225.2
Methoxymalonyl-CoA: 185.4
2-Methylbutyryl-CoA: 157.2
trans-1,2-CPDA: 142.4
Propionyl-CoA: 114.6
CHC-CoA: 106.7
Malonyl-CoA: 103.9
Isobutyryl-CoA: 93.4
Benzoyl-CoA: 67.8
Acetyl-CoA: 64.0
fatty_acid: 51.7
3-Methylbutyryl-CoA: 40.1
inactive: 30.0

FED44_RS26725: pk - Ser

Search NORINE for peptide: strict or relaxed

PKS_AT (552..850): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 174.7
Ethylmalonyl-CoA: 154.5
Methoxymalonyl-CoA: 140.9
trans-1,2-CPDA: 82.8
2-Methylbutyryl-CoA: 79.8
Isobutyryl-CoA: 73.4
Propionyl-CoA: 71.3
Malonyl-CoA: 69.4
CHC-CoA: 66.0
Benzoyl-CoA: 53.0
Acetyl-CoA: 30.8
fatty_acid: 27.2
inactive: 17.9
3-Methylbutyryl-CoA: 13.1

AMP-binding (1515..1914): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWQTFDVSVWEWHFIASGEYNLWGATECSVDSTC
Nearest Stachelhaus code(s):: Ser DVWHISLVDK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

FED44_RS26730: mmal

PKS_AT (572..881): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 187.3
Ethylmalonyl-CoA: 160.6
Methoxymalonyl-CoA: 140.4
2-Methylbutyryl-CoA: 104.5
trans-1,2-CPDA: 92.8
Malonyl-CoA: 91.5
CHC-CoA: 88.3
Propionyl-CoA: 82.9
Isobutyryl-CoA: 80.3
Benzoyl-CoA: 60.4
Acetyl-CoA: 37.9
fatty_acid: 29.3
3-Methylbutyryl-CoA: 23.2
inactive: 17.5

FED44_RS26735: mmal - mmal - mmal

PKS_AT (92..387): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.4
Ethylmalonyl-CoA: 191.7
Methoxymalonyl-CoA: 167.8
2-Methylbutyryl-CoA: 143.0
Propionyl-CoA: 130.9
Malonyl-CoA: 130.5
CHC-CoA: 119.9
trans-1,2-CPDA: 115.4
Isobutyryl-CoA: 70.4
Benzoyl-CoA: 58.1
Acetyl-CoA: 56.7
fatty_acid: 42.2
inactive: 34.9
3-Methylbutyryl-CoA: 34.9

PKS_AT (1543..1809): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 206.5
Ethylmalonyl-CoA: 195.0
Methoxymalonyl-CoA: 163.8
2-Methylbutyryl-CoA: 128.2
trans-1,2-CPDA: 117.0
Propionyl-CoA: 100.6
CHC-CoA: 95.6
Malonyl-CoA: 95.5
Isobutyryl-CoA: 77.8
Benzoyl-CoA: 63.1
3-Methylbutyryl-CoA: 42.3
Acetyl-CoA: 36.2
fatty_acid: 31.3
inactive: 23.9

PKS_AT (3194..3466): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.2
Ethylmalonyl-CoA: 208.3
Methoxymalonyl-CoA: 173.5
2-Methylbutyryl-CoA: 144.6
trans-1,2-CPDA: 122.8
Propionyl-CoA: 115.0
CHC-CoA: 111.1
Malonyl-CoA: 102.1
Isobutyryl-CoA: 77.1
Benzoyl-CoA: 60.9
Acetyl-CoA: 44.0
3-Methylbutyryl-CoA: 37.2
fatty_acid: 30.3
inactive: 20.4

FED44_RS26740: mmal

PKS_AT (576..849): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 201.0
Ethylmalonyl-CoA: 182.0
Methoxymalonyl-CoA: 165.4
2-Methylbutyryl-CoA: 140.8
Malonyl-CoA: 119.6
Propionyl-CoA: 113.5
CHC-CoA: 111.8
trans-1,2-CPDA: 109.5
Isobutyryl-CoA: 61.9
Acetyl-CoA: 53.8
Benzoyl-CoA: 53.2
fatty_acid: 39.4
3-Methylbutyryl-CoA: 38.4
inactive: 37.8

FED44_RS26745: emal - mmal

PKS_AT (124..422): emal

ATSignature: 2-Methylbutyryl-CoA

Top 3 matches:: 2-Methylbutyryl-CoA: 70.8%; Isobutyryl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 154.1
2-Methylbutyryl-CoA: 152.0
Methylmalonyl-CoA: 151.8
Methoxymalonyl-CoA: 126.0
Propionyl-CoA: 120.1
trans-1,2-CPDA: 102.5
CHC-CoA: 100.9
Malonyl-CoA: 68.7
Isobutyryl-CoA: 68.4
Benzoyl-CoA: 45.2
Acetyl-CoA: 27.7
3-Methylbutyryl-CoA: 27.5
fatty_acid: 26.6
inactive: 21.7

PKS_AT (1117..1417): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.9
Ethylmalonyl-CoA: 208.8
Methoxymalonyl-CoA: 181.5
2-Methylbutyryl-CoA: 139.6
trans-1,2-CPDA: 131.0
Propionyl-CoA: 100.8
CHC-CoA: 100.1
Malonyl-CoA: 98.9
Isobutyryl-CoA: 82.9
Benzoyl-CoA: 56.4
Acetyl-CoA: 44.1
inactive: 39.6
3-Methylbutyryl-CoA: 32.1
fatty_acid: 31.8

FED44_RS26750: mal

PKS_AT (545..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 192.7
inactive: 94.2
Methoxymalonyl-CoA: 72.1
Methylmalonyl-CoA: 63.8
Propionyl-CoA: 38.7
Isobutyryl-CoA: 33.4
Ethylmalonyl-CoA: 29.0
Benzoyl-CoA: 22.2
fatty_acid: 19.5
2-Methylbutyryl-CoA: 19.4
Acetyl-CoA: 19.0
3-Methylbutyryl-CoA: 15.9
trans-1,2-CPDA: 13.2
CHC-CoA: 8.9

FED44_RS26935: mal

PKS_AT (1497..1805): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.2
inactive: 112.8
Methoxymalonyl-CoA: 73.0
Methylmalonyl-CoA: 55.6
Propionyl-CoA: 37.1
Isobutyryl-CoA: 36.7
Acetyl-CoA: 29.9
Benzoyl-CoA: 28.2
Ethylmalonyl-CoA: 23.8
fatty_acid: 10.2
trans-1,2-CPDA: 8.4
2-Methylbutyryl-CoA: 6.6
CHC-CoA: 5.7
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
CelR	Cellobiose uptake repressor
IolR	Regulator of the inositol catabolism
MexT	Global virulence regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_VANP01000014.1 - Region 1 - PKS-like,T1PKS

Download region GenBank file

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Location: 24,942 - 119,917 nt. (total: 94,976 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

FED44_RS28475: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

FED44_RS28665: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

FED44_RS28705: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

FED44_RS28720: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

FED44_RS28730: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acyltransferase

FED44_RS28715: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Hydrolases: esterase hydrolase phosphatase

FED44_RS28500: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

FED44_RS28580: thioesterase

smcogs: SMCOG1004: thioesterase

FED44_RS28675: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

Lyases: cyclase

FED44_RS28735: Diels-Alderase cyclase

extras: DA_cyclase: Diels-Alderase cyclase

Ligases: synthase

FED44_RS28480: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

FED44_RS28485: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

FED44_RS28495: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

FED44_RS28560: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

FED44_RS28670: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

FED44_RS37770: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.78	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.78	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002175.3	0.59	PKS	YWA1	Aspergillus oryzae RIB40
BGC0000037.4	0.58	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001284.4	0.57	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.56	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.78	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.78	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.78	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.77	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001492.5	0.71	PKS	abyssomicin M, abyssomicin N, abyssomicin O, abyssomicin P, abyssomicin Q, abyssomicin R, abyssomicin S, abyssomicin T, abyssomicin U, abyssomicin V, abyssomicin W, abyssomicin X	Streptomyces sp. LC-6-2
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000041.3	0.63	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 24.07 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 26.03 out of 30.11

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 21.07 out of 25.31

Confidence: medium

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 27.51 out of 30.97

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (27):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.02 out of 18.57

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.19 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 15.56 out of 31.73

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.94 out of 26.85

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.01 out of 20.97

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.48 out of 19.26

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.77 out of 21.55

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.82 out of 26.06

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.08 out of 20.97

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 18.81 out of 27.55

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.54 out of 30.97

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.04 out of 19.26

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.20 out of 21.55

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 16.96 out of 22.40

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 25.75

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.88 out of 25.75

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.15 out of 26.06

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.97 out of 30.98

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 22.60 out of 35.63

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.50 out of 35.63

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.38 out of 23.43

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.72 out of 28.14

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.10 out of 29.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.26 out of 18.57

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.59 out of 25.88

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.14 out of 27.04

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.04 out of 20.01

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 24941 - 119917:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal - Me-ccmal - ccmal - ccmal) + (Me-mal - Me-redmal) + (mal) + (pk)
Non-colinear ordering:: FED44_RS37770 + FED44_RS28665 + FED44_RS28670 + FED44_RS28480

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS28480: pk

PKS_AT (529..819): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 75.0%; Ethylmalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 123.1
Ethylmalonyl-CoA: 106.0
Methoxymalonyl-CoA: 98.5
Malonyl-CoA: 93.8
Isobutyryl-CoA: 65.0
Propionyl-CoA: 58.0
trans-1,2-CPDA: 57.7
2-Methylbutyryl-CoA: 54.2
Benzoyl-CoA: 48.9
Acetyl-CoA: 39.3
3-Methylbutyryl-CoA: 34.5
inactive: 28.4
fatty_acid: 27.4
CHC-CoA: 21.8

FED44_RS37770: mal - mmal - mal - mal

PKS_AT (550..854): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 186.1
inactive: 99.9
Methoxymalonyl-CoA: 74.0
Methylmalonyl-CoA: 72.0
Isobutyryl-CoA: 44.2
Ethylmalonyl-CoA: 38.7
Propionyl-CoA: 36.0
Benzoyl-CoA: 28.8
fatty_acid: 16.6
CHC-CoA: 16.6
2-Methylbutyryl-CoA: 10.0
trans-1,2-CPDA: 7.7
3-Methylbutyryl-CoA: 0.0
Acetyl-CoA: -1.1

PKS_AT (1642..1938): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 209.4
Ethylmalonyl-CoA: 205.6
Methoxymalonyl-CoA: 164.4
2-Methylbutyryl-CoA: 124.8
trans-1,2-CPDA: 123.3
CHC-CoA: 100.7
Propionyl-CoA: 99.4
Malonyl-CoA: 85.1
Isobutyryl-CoA: 82.5
Benzoyl-CoA: 67.8
3-Methylbutyryl-CoA: 45.2
Acetyl-CoA: 36.9
inactive: 20.9
fatty_acid: 20.0

PKS_AT (3480..3764): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 181.9
inactive: 70.8
Methoxymalonyl-CoA: 59.7
Methylmalonyl-CoA: 54.7
Propionyl-CoA: 31.6
Ethylmalonyl-CoA: 22.2
Isobutyryl-CoA: 19.1
Benzoyl-CoA: 17.8
2-Methylbutyryl-CoA: 17.7
Acetyl-CoA: 14.1
fatty_acid: 11.1
trans-1,2-CPDA: 10.9
CHC-CoA: 8.1
3-Methylbutyryl-CoA: 1.7

PKS_AT (5225..5508): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.1
inactive: 72.4
Methoxymalonyl-CoA: 57.3
Methylmalonyl-CoA: 55.6
Propionyl-CoA: 22.0
Ethylmalonyl-CoA: 19.4
CHC-CoA: 17.4
Acetyl-CoA: 17.3
trans-1,2-CPDA: 15.0
Isobutyryl-CoA: 15.0
2-Methylbutyryl-CoA: 13.4
fatty_acid: 11.9
Benzoyl-CoA: 10.1
3-Methylbutyryl-CoA: 0.0

FED44_RS28665: mmal - mmal

PKS_AT (571..870): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.4
Ethylmalonyl-CoA: 187.5
Methoxymalonyl-CoA: 170.7
2-Methylbutyryl-CoA: 123.7
trans-1,2-CPDA: 115.3
Propionyl-CoA: 103.1
Malonyl-CoA: 96.2
CHC-CoA: 96.2
Isobutyryl-CoA: 93.4
3-Methylbutyryl-CoA: 56.6
Acetyl-CoA: 48.8
Benzoyl-CoA: 46.8
inactive: 30.4
fatty_acid: 26.6

PKS_AT (1987..2289): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.4
Ethylmalonyl-CoA: 167.6
Methoxymalonyl-CoA: 157.1
trans-1,2-CPDA: 110.9
2-Methylbutyryl-CoA: 97.7
Propionyl-CoA: 93.2
Malonyl-CoA: 81.3
CHC-CoA: 69.4
Isobutyryl-CoA: 57.9
Benzoyl-CoA: 46.7
Acetyl-CoA: 40.5
3-Methylbutyryl-CoA: 39.7
inactive: 26.8
fatty_acid: 18.6

FED44_RS28670: mal

PKS_AT (577..882): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Propionyl-CoA: 58.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 141.3
Methoxymalonyl-CoA: 62.1
inactive: 60.1
Methylmalonyl-CoA: 48.5
Propionyl-CoA: 38.9
2-Methylbutyryl-CoA: 32.1
Isobutyryl-CoA: 14.2
fatty_acid: 13.6
CHC-CoA: 13.0
Ethylmalonyl-CoA: 11.6
Acetyl-CoA: 8.6
3-Methylbutyryl-CoA: 6.3
trans-1,2-CPDA: 6.2
Benzoyl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
CelR	Cellobiose uptake repressor
CopR	Copper-responsive regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_VANP01000014.1 - Region 2 - NRPS

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Location: 172,133 - 207,844 nt. (total: 35,712 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

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resistance

TTA codons

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NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase oxygenase phosphate reductase

FED44_RS28960: N-acetyl-gamma-glutamyl-phosphate reductase

smcogs: SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase

FED44_RS29015: dioxygenase, TauD/TfdA

smcogs: SMCOG1121: dioxygenase, TauD/TfdA

FED44_RS29020: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

Transferases: aminotransferase kinase transketolase

FED44_RS28955: acetylglutamate kinase

smcogs: SMCOG1174: acetylglutamate kinase

FED44_RS28980: transketolase

smcogs: SMCOG1204: transketolase

FED44_RS28985: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: esterase

FED44_RS29055: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

FED44_RS28975: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

FED44_RS29030: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS29040: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS29050: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS29060: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS29065: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.71	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.70	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.71	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.70	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

Additional weak hits (8):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.29 out of 28.14

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.78 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.53 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.72 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.42 out of 29.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.10 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 172132 - 207844:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala) + (NH2) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS29030: X

AMP-binding (297..723): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SARMFDGAAGGWHSSVGGELNDYGPTECAVWATA
Nearest Stachelhaus code(s):: dohmTyr DGGHAGDVWK (85% 8Å match); mTyr or ohdmTyr DGSHSGDVWK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

FED44_RS29040: X

AMP-binding (490..887): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AARAFDAAGGGWDGVVAGEFNEYGPTEATVWASV
Nearest Stachelhaus code(s):: 4,5-dhArg DAGDIAEVWK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

FED44_RS29060: NH2

CAL_domain (147..445): NH2

minowa: NH2

Prediction, score:

NH2: 126.4
Acetyl-CoA: 38.8
fatty_acid: 34.2
AHBA: 10.3
shikimic_acid: 0.0

FED44_RS29065: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (499..919): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: MWATFDLSVFEANTNLAGENNLYGPTEDTTYSTH
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_VANP01000015.1 - Region 1 - NI-siderophore

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Location: 29,672 - 61,007 nt. (total: 31,336 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

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SubClusterBlast

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Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

FED44_RS29250: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

Lyases: decarboxylase

FED44_RS29275: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000946.5	0.54	other (other)	vibrioferrin	Vibrio parahaemolyticus
BGC0002527.2	0.54	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0001408.5	0.53	other (other)	xanthoferrin	Xanthomonas oryzae pv. oryzae KACC 10331
BGC0000947.5	0.44	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0001364.3	0.40	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0000943.5	0.40	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002313.3	0.39	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000772.5	0.39	saccharide	lipopolysaccharide	Brucella melitensis
BGC0001870.3	0.38	other (other)	putrebactin, avaroferrin	Xenorhabdus budapestensis
BGC0000563.4	0.37	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000946.5	0.54	other (other)	vibrioferrin	Vibrio parahaemolyticus
BGC0002527.2	0.54	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0001408.5	0.53	other (other)	xanthoferrin	Xanthomonas oryzae pv. oryzae KACC 10331
BGC0000947.5	0.44	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0001364.3	0.40	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0000943.5	0.40	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002313.3	0.39	other (other)	streptozotocin	Streptomyces achromogenes subsp. streptozoticus
BGC0000772.5	0.39	saccharide	lipopolysaccharide	Brucella melitensis
BGC0001870.3	0.38	other (other)	putrebactin, avaroferrin	Xenorhabdus budapestensis
BGC0000563.4	0.37	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.18 out of 20.47

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.54 out of 23.79

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.52 out of 19.26

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.17 out of 25.31

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.86 out of 36.53

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.58 out of 23.79

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (14):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.82 out of 30.25

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.95 out of 21.55

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.41 out of 31.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.70 out of 21.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.78 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.74 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.45 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.36 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.45 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.09 out of 27.78

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.17 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.44 out of 36.54

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.98 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.52 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor
DmdR1	Iron(II)-dependent repressor
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_VANP01000016.1 - Region 1 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 59,322 - 80,389 nt. (total: 21,068 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

FED44_RS30285: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

FED44_RS30290: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

Transferases: carbamoyltransferase phosphorylase

FED44_RS30225: ornithine carbamoyltransferase

smcogs: SMCOG1114: ornithine carbamoyltransferase

FED44_RS30275: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

Ligases: synthetase

FED44_RS30270: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001521.3	0.41	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0000664.5	0.41	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0002345.2	0.40	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000906.3	0.39	other (other)	heme D1	Heliophilum fasciatum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001521.3	0.41	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0001456.3	0.41	terpene	isorenieratene	Streptomyces argillaceus
BGC0000664.5	0.41	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.40	terpene	carotenoid	Streptomyces avermitilis
BGC0002345.2	0.40	other (other)	hydrogen cyanide	Pseudomonas fluorescens
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000906.3	0.39	other (other)	heme D1	Heliophilum fasciatum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

FED44_RS30270: PT_FPPS_like

Domain 1 (47 - 347): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 25.49 out of 29.65

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.00 out of 24.78

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.27 out of 24.78

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 19.86 out of 21.84

Confidence: medium

Additional weak hits (4):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.29 out of 18.57

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.43 out of 21.55

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.51 out of 35.63

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.78 out of 29.25

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response
MexT	Global virulence regulator

NZ_VANP01000017.1 - Region 1 - NRPS

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Download region SVG

Location: 124,013 - 159,191 nt. (total: 35,179 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase electron transfer oxidoreductase oxygenase

FED44_RS31360: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

FED44_RS31365: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

FED44_RS31370: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

FED44_RS31400: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

FED44_RS31410: flavodoxin

smcogs: SMCOG1134: flavodoxin

FED44_RS31450: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

FED44_RS31465: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

FED44_RS31470: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

Transferases: glycosyltransferase

FED44_RS31460: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

Hydrolases: hydrolase phosphatase

FED44_RS31455: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

FED44_RS31475: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase

FED44_RS31445: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.68 out of 20.47

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.37 out of 26.85

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 30.11 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

Additional weak hits (8):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.51 out of 26.85

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.29 out of 21.55

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.81 out of 28.14

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.69 out of 21.55

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.59 out of 32.74

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.79 out of 22.40

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.15 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 124012 - 159191:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS31445: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (508..903): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HWAGFDLVVPNAKCVVAGEVNEYGPTEASVGTCI
Nearest Stachelhaus code(s):: D-Ala DLTKVNEVGK (71% 8Å match); Asn DLTKVGEVGK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CelR	Cellobiose uptake repressor
RutR	Regulator of the pyrimidine and purine metabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_VANP01000018.1 - Region 1 - T1PKS

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Location: 1 - 65,339 nt. (total: 65,339 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

FED44_RS31560: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase aminotransferase

FED44_RS31490: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

FED44_RS31510: aminotransferase

smcogs: SMCOG1019: aminotransferase

FED44_RS31520: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

FED44_RS31545: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: dehalogenase esterase

FED44_RS31500: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

FED44_RS31580: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

FED44_RS31530: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS31535: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS31540: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

FED44_RS38255: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.73	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.73	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0000041.3	0.63	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.73	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.73	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.73	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0000041.3	0.63	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.49 out of 20.97

Confidence: medium

Additional weak hits (24):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.67 out of 27.78

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.34 out of 26.12

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 12.78 out of 28.14

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.76 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.08 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.91 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 19.90 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.35 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.29 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.41 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.20 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.66 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.06 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.59 out of 27.78

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.84 out of 30.22

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.67 out of 30.22

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.64 out of 30.97

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.20 out of 39.62

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.53 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.79 out of 21.84

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.91 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.60 out of 29.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.31 out of 35.63

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.50 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 65339:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ccmal - ccmal) + (ccmal) + (ccmal - ccmal)
Non-colinear ordering:: FED44_RS31540 + FED44_RS31545 + FED44_RS38255

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS38255: mal - mal

PKS_AT (563..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.5
inactive: 114.8
Methoxymalonyl-CoA: 58.6
Methylmalonyl-CoA: 55.9
Isobutyryl-CoA: 30.0
Acetyl-CoA: 25.8
Ethylmalonyl-CoA: 24.0
Propionyl-CoA: 21.1
fatty_acid: 19.0
Benzoyl-CoA: 16.4
CHC-CoA: 14.8
trans-1,2-CPDA: 8.2
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.3

PKS_AT (2351..2641): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.0
inactive: 113.8
Methoxymalonyl-CoA: 59.9
Methylmalonyl-CoA: 58.6
Ethylmalonyl-CoA: 26.4
Propionyl-CoA: 23.4
Isobutyryl-CoA: 22.4
Acetyl-CoA: 21.4
trans-1,2-CPDA: 18.0
fatty_acid: 17.9
Benzoyl-CoA: 17.7
CHC-CoA: 12.8
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.4

FED44_RS31520: pk

PKS_AT (39..278): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 13.1
Methylmalonyl-CoA: 10.8
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

FED44_RS31530: X

AMP-binding (18..410): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YLMFFDCAMFDCFSLFAGEENIYGPTELTITVTG
Nearest Stachelhaus code(s):: bMe-Asp DCFFFALITK (82% 8Å match); Ala DCFFFALITK (79% 8Å match)
Stachelhaus code match:: 80% (moderate)

FED44_RS31535: X

AMP-binding (2..411): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGMSFDFSLLDLNGLYSGEVNCFGSTESIAASLT
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

FED44_RS31540: mal - mal - mal

PKS_AT (705..1003): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.8
inactive: 113.7
Methoxymalonyl-CoA: 67.9
Methylmalonyl-CoA: 54.0
Isobutyryl-CoA: 30.4
Propionyl-CoA: 27.6
Acetyl-CoA: 25.0
Ethylmalonyl-CoA: 13.6
fatty_acid: 13.5
Benzoyl-CoA: 12.9
CHC-CoA: 7.7
trans-1,2-CPDA: 7.3
2-Methylbutyryl-CoA: 5.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (2423..2711): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.2
inactive: 107.4
Methoxymalonyl-CoA: 63.7
Methylmalonyl-CoA: 63.3
Propionyl-CoA: 41.6
Isobutyryl-CoA: 39.1
Acetyl-CoA: 31.0
Ethylmalonyl-CoA: 24.2
CHC-CoA: 14.7
Benzoyl-CoA: 13.5
fatty_acid: 9.8
trans-1,2-CPDA: 7.6
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -2.1

PKS_AT (4200..4499): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 209.3
inactive: 96.4
Methoxymalonyl-CoA: 60.8
Methylmalonyl-CoA: 52.1
Isobutyryl-CoA: 25.3
Ethylmalonyl-CoA: 17.0
Propionyl-CoA: 16.8
Acetyl-CoA: 16.2
fatty_acid: 9.3
Benzoyl-CoA: 8.1
CHC-CoA: 6.3
2-Methylbutyryl-CoA: 2.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

FED44_RS31545: mal

PKS_AT (554..843): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 189.8
inactive: 89.9
Methoxymalonyl-CoA: 57.9
Methylmalonyl-CoA: 49.8
Propionyl-CoA: 23.2
Isobutyryl-CoA: 22.4
Acetyl-CoA: 21.7
Ethylmalonyl-CoA: 20.4
Benzoyl-CoA: 13.3
CHC-CoA: 12.2
fatty_acid: 9.4
trans-1,2-CPDA: 4.7
2-Methylbutyryl-CoA: 1.4
3-Methylbutyryl-CoA: -1.5

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
ToxT	Regulator of MSHA biosynthesis

NZ_VANP01000018.1 - Region 2 - redox-cofactor

Download region GenBank file

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Location: 67,442 - 90,290 nt. (total: 22,849 nt) Show pHMM detection rules used

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

FED44_RS31650: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: halogenase transferase

FED44_RS31635: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

FED44_RS31695: halogenase

smcogs: SMCOG1119: halogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.52	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002493.3	0.41	NRPS	parabactin	Paracoccus denitrificans PD1222
BGC0002584.2	0.40	ribosomal	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0002218.2	0.39	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0001221.5	0.38	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0002284.3	0.37	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000122.5	0.36	PKS	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001444.5	0.36	other (shikimate-derived)	caboxamycin	Streptomyces sp. NTK 937
BGC0000877.5	0.35	other (other)	polyoxin A, polyoxin H	Streptomyces cacaoi subsp. asoensis
BGC0001366.5	0.35	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.52	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002493.3	0.41	NRPS	parabactin	Paracoccus denitrificans PD1222
BGC0002584.2	0.40	ribosomal	azolemycin B, azolemycin A, azolemycin D, azolemycin C	Streptomyces sp. FXJ1.264
BGC0002218.2	0.39	terpene	(-)-Cyatha-3,12-diene	Dentipellis sp. KUC8613
BGC0001221.5	0.38	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0002284.3	0.37	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000122.5	0.36	PKS	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0001444.5	0.36	other (shikimate-derived)	caboxamycin	Streptomyces sp. NTK 937
BGC0000877.5	0.35	other (other)	polyoxin A, polyoxin H	Streptomyces cacaoi subsp. asoensis
BGC0001366.5	0.35	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 20.47

Confidence: medium

Additional weak hits (9):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.68 out of 30.22

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.30 out of 20.01

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.23 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.43 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.11 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.84 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.13 out of 26.06

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.49 out of 25.88

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Mycofactocin_RRE; Location: 5 - 85; Score: 94.1

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_VANP01000019.1 - Region 1 - terpene

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Location: 21,940 - 42,908 nt. (total: 20,969 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

FED44_RS32025: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Hydrolases: hydrolase

FED44_RS32060: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: cyclase

FED44_RS32015: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001746.3	0.75	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0001201.3	0.51	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0000675.5	0.45	terpene	(+)-T-muurolol	Streptomyces clavuligerus ATCC 27064
BGC0001387.5	0.41	other (other)	nucleocidin	Streptomyces calvus
BGC0001294.5	0.41	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002318.2	0.40	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0000528.5	0.36	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002509.3	0.35	NRPS	montamide A, capsimycin, clifednamide A, frontalamide A, combamide A	Streptomyces sp.
BGC0001972.4	0.35	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001746.3	0.75	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0001201.3	0.51	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0000675.5	0.45	terpene	(+)-T-muurolol	Streptomyces clavuligerus ATCC 27064
BGC0001387.5	0.41	other (other)	nucleocidin	Streptomyces calvus
BGC0001294.5	0.41	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002318.2	0.40	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0000528.5	0.36	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002316.2	0.36	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0002509.3	0.35	NRPS	montamide A, capsimycin, clifednamide A, frontalamide A, combamide A	Streptomyces sp.
BGC0001972.4	0.35	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

FED44_RS32015: T1TS

Domain 1 (11 - 317): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.16 out of 26.33

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.67 out of 25.31

Confidence: medium

Additional weak hits (9):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 19.96 out of 27.18

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.86 out of 19.52

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.42 out of 20.97

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.82 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.00 out of 27.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.28 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.47 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CatR	H2O2-responsive repressor

NZ_VANP01000002.1 - Region 1 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 2,919 - 25,795 nt. (total: 22,877 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: protease

FED44_RS05235: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase dehydratase

FED44_RS05220: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

FED44_RS05265: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: mutase

FED44_RS05170: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.52	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000503.5	0.47	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002700.2	0.45	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0001407.5	0.45	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0002346.3	0.45	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0001789.3	0.44	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000536.3	0.44	ribosomal	nisin Q	Lactococcus lactis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000528.5	0.52	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000503.5	0.47	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002700.2	0.45	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0001407.5	0.45	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0002346.3	0.45	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0001789.3	0.44	ribosomal	suicin 90-1330	Streptococcus suis
BGC0000536.3	0.44	ribosomal	nisin Q	Lactococcus lactis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for FED44_RS05220

FED44_RS05225

Leader:

MPLTLDRSEEIVSAWRSGADVEGWDSPAG

Core:

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

Core with dehydrations:

PLFIGEYAEDhaEIDhbLDhbALDDhbRLADhaDhaCysDhbCysDhaRFCysCys

antiSMASH-DB 4.0 matches for FED44_RS05225

100.0%

NZ_JABBWY010000002.1and 3 others:

NZ_JABBWZ010000011.1 Lanthipeptides: HHX40_RS26720_lanthipeptideNZ_VANP01000002.1 Lanthipeptides: FED44_RS05225_lanthipeptideNZ_VYTZ01000002.1 Lanthipeptides: F5972_RS06340_lanthipeptide

Lanthipeptides: HHX37_RS08025_lanthipeptide

1

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

32

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

1

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

32

66.0%

NZ_BMQJ01000023.1:

Lanthipeptides: IEZ10_RS33030_lanthipeptide

1

PLFIGEYAESEITLTALDTRLASSCTCSRFCC

32

PLFIG+YAESEITLTALDTRLASSCTCSRFCC

16

PLFIGDYAESEITLTALDTRLASSCTCSRFCC

47

49.0%

NZ_JABBWY010000002.1and 3 others:

NZ_JABBWZ010000011.1 Lanthipeptides: HHX40_RS26715_lanthipeptideNZ_VANP01000002.1 Lanthipeptides: FED44_RS05230_lanthipeptideNZ_VYTZ01000002.1 Lanthipeptides: F5972_RS06335_lanthipeptide

Lanthipeptides: HHX37_RS08020_lanthipeptide

1

PLFIGEYAESEITLTALDTRLASSCT

26

PLFIGEYAESEITLTALDTR SSCT

16

PLFIGEYAESEITLTALDTRRPSSCT

41

with 26 more sequence matches

Only the first 10 are displayed here.

45.7%

NZ_FWXV01000001.1:

Lanthipeptides: B9C43_RS03005_lanthipeptide

1

PLFI-GEYAESEITLTALDTRLASSCTCSR--FCC

32

P+F GE+AE +I SSCT SR FCC

1

PIFASGEFAEGDIVACNPPGSRCSSCTASRPVFCC

35

40.5%

NZ_CP048405.1:

Lanthipeptides: GXW83_RS14070_lanthipeptide

1

PLFI-GEYAESEITLTALDTRLASSCTCS----RFCC

32

PLF G YAESE+T + + CS R CC

1

PLFTSGAYAESELTASGAVRTIQCGTACSYSAGRECC

37

40.0%

NZ_FOZI01000001.1:

Lanthipeptides: BM338_RS00935_lanthipeptide

1

PLFIG-EYAESEI-----TLTALDTRLASSCTCS--RFCC

32

PLF G ++AE+EI +LT ++CT S R+CC

2

PLFTGGQFAEAEIISEASSLTTFG-GCGTACTGSHTRYCC

40

38.9%

NZ_AZWQ01000024.1:

Lanthipeptides: B116_RS0113780_lanthipeptide

1

PLFIG-EYAESEITLTALD-TRLASSCTCSR

29

PLF G +Y EIT T + + SSCT S+

1

PLFTGGKYVVQEITSTWGGCSTVCSSCTGSQ

31

37.8%

NZ_AZVD01000036.1and 1 other:

NZ_KB913036.1 Lanthipeptides: A39M_RS0105590_lanthipeptide

Lanthipeptides: K389_RS0119060_lanthipeptide

1

PLFIG-EYAESEITLTAL-DTRLASSCTCS

28

PLF G Y EIT TA+ + S+CT S

1

PLFTGGRYVVQEITATAVANWTYCSACTGS

30

37.1%

NZ_JANYMP010000013.1:

Lanthipeptides: NZH93_RS25860_lanthipeptide

1

PLFI-GEYAESEITLTALDTRLASSCTCSR

29

PLF GE+AE +I S+CT SR

1

PLFASGEFAEGDIVACNPPGSRCSACTASR

30

37.0%

NZ_QFWW01000008.1:

Lanthipeptides: DI276_RS14925_lanthipeptide

1

PLFI-GEYAESEITLTALDTRLASSCTCS--RFCC

32

PLF GEYAE E+T+T + + CT S R CC

12

PLFPSGEYAEHELTMTGHSGKCGTVCTYSQGRQCC

46

36.1%

NZ_JALPRT010000019.1and 1 other:

NZ_KB895919.1 Lanthipeptides: C570_RS1000000130195_lanthipeptide

Lanthipeptides: M0M45_RS13255_lanthipeptide

1

PLFIG-EYAESEITLTALD-TRLASSCT

26

PLF G Y EIT T + + SSCT

1

PLFTGGRYVVQEITSTWGGCSTVCSSCT

28

35.3%

NZ_CP034550.1:

Lanthipeptides: EKG83_RS30035_lanthipeptide

5

GEYAESEITLTALDTRLASSCTCSR--FCC

32

GE+AE +I SSCT S +CC

5

GEFAEGDIVACNPPGSRCSSCTASAHVYCC

34

34.7%

NZ_QOIL01000017.1:

Lanthipeptides: DQ384_RS40545_lanthipeptide

1

PLFIGEYAESEITLTALDTRLASSCTC-SRFCC

32

P IGEY E EIT++ ++T+ SSCT FCC

17

PPLIGEYTEEEITMSLIETKRPSSCTAGGGFCC

49

FED44_RS05230

Leader:

MPVTLDRSEEIVSAWRSGADVEGWDSPAG

Core:

PLFIGEYAESEITLTALDTRRPSSCTGANTIPCC

Core with dehydrations:

PLFIGEYAEDhaEIDhbLDhbALDDhbRRPDhaDhaCysDhbGANDhbIPCysCys

antiSMASH-DB 4.0 matches for FED44_RS05230

70.6%

NZ_JABBWY010000002.1and 3 others:

NZ_JABBWZ010000011.1 Lanthipeptides: HHX40_RS26720_lanthipeptideNZ_VANP01000002.1 Lanthipeptides: FED44_RS05225_lanthipeptideNZ_VYTZ01000002.1 Lanthipeptides: F5972_RS06340_lanthipeptide

Lanthipeptides: HHX37_RS08025_lanthipeptide

1

PLFIGEYAESEITLTALDTRRPSSCT

26

PLFIGEYAESEITLTALDTR SSCT

1

PLFIGEYAESEITLTALDTRLASSCT

26

69.4%

NZ_JABBWY010000002.1and 3 others:

NZ_JABBWZ010000011.1 Lanthipeptides: HHX40_RS26715_lanthipeptideNZ_VANP01000002.1 Lanthipeptides: FED44_RS05230_lanthipeptideNZ_VYTZ01000002.1 Lanthipeptides: F5972_RS06335_lanthipeptide

Lanthipeptides: HHX37_RS08020_lanthipeptide

1

PLFIGEYAESEITLTALDTRRPSSCTGANTIPCC

34

PLFIGEYAESEITLTALDTRRPSSCTGANTIPCC

16

PLFIGEYAESEITLTALDTRRPSSCTGANTIPCC

49

48.9%

NZ_BMQJ01000023.1:

Lanthipeptides: IEZ10_RS33030_lanthipeptide

1

PLFIGEYAESEITLTALDTRRPSSCT

26

PLFIG+YAESEITLTALDTR SSCT

16

PLFIGDYAESEITLTALDTRLASSCT

41

with 39 more sequence matches

Only the first 10 are displayed here.

48.6%

NZ_JANZKX010000002.1:

Lanthipeptides: O9Y01_RS08280_lanthipeptide

1

PLFI-GEYAESEITL-TALDTRRP-SSCTGANTIPCC

34

PLF G Y+E+EI + +TR+ SSC+G+ TI CC

1

PLFSSGVYSEAEIAGDVSRETRQGCSSCSGSRTIMCC

37

47.2%

NZ_JALPRT010000019.1and 1 other:

NZ_KB895919.1 Lanthipeptides: C570_RS1000000130195_lanthipeptide

Lanthipeptides: M0M45_RS13255_lanthipeptide

1

PLFIG-EYAESEITLTALD-TRRPSSCTGANTIPCC

34

PLF G Y EIT T + SSCTG+ I CC

1

PLFTGGRYVVQEITSTWGGCSTVCSSCTGSVPIQCC

36

44.4%

NZ_AZWQ01000024.1:

Lanthipeptides: B116_RS0113780_lanthipeptide

1

PLFIG-EYAESEITLTALD-TRRPSSCTGANTIPCC

34

PLF G +Y EIT T + SSCTG+ + CC

1

PLFTGGKYVVQEITSTWGGCSTVCSSCTGSQRVSCC

36

41.5%

NZ_CP079946.1:

Lanthipeptides: KYK13_RS20175_lanthipeptide

1

PLFIG----EYAESEITLTALDTRRPSSCTGANTIPCC

34

P+F+G E A +E L A DT SSCT + T CC

6

PVFVGGTSTEKALTESNLVA-DTGC-SSCTASTTAFCC

41

40.0%

NZ_FWXV01000001.1:

Lanthipeptides: B9C43_RS03005_lanthipeptide

1

PLFI-GEYAESEITLTALDTRRPSSCTGANTIPCC

34

P+F GE+AE +I R SSCT + + CC

1

PIFASGEFAEGDIVACNPPGSRCSSCTASRPVFCC

35

40.0%

NZ_JANYMP010000013.1:

Lanthipeptides: NZH93_RS25860_lanthipeptide

1

PLFI-GEYAESEITLTALDTRRPSSCTGANTIPCC

34

PLF GE+AE +I R S+CT + + CC

1

PLFASGEFAEGDIVACNPPGSRCSACTASRPVVCC

35

39.5%

NZ_CP064030.1:

Lanthipeptides: ISN74_RS08625_lanthipeptide

1

PLFI-GEYAESEI-------TLTALDTRR-PSSCTGANTIPCC

34

PLF+ G++AES+I TL A+ + R S+C+G+ CC

1

PLFVSGQFAESDIVSSTNVLTLGAVCSGRCGSACSGSAHAECC

43

37.8%

NZ_AZVD01000036.1and 1 other:

NZ_KB913036.1 Lanthipeptides: A39M_RS0105590_lanthipeptide

Lanthipeptides: K389_RS0119060_lanthipeptide

1

PLFIG-EYAESEITLTAL-DTRRPSSCTGAN

29

PLF G Y EIT TA+ + S+CTG+

1

PLFTGGRYVVQEITATAVANWTYCSACTGSG

31

37.5%

NZ_CP072597.1:

Lanthipeptides: J5226_RS01600_lanthipeptide

1

PLFI-GEYAESEITL-TALDTRRPSSCTGANTIPCC

34

PLFI GE+AE++I + +R S+CTG TI CC

13

PLFISGEFAEADIAGDNEIISRNTSACTGCGTIQCC

48

37.5%

NZ_FOZI01000001.1:

Lanthipeptides: BM338_RS00935_lanthipeptide

1

PLFIG-EYAESEITLTALDTRR----PSSCTGANTIPCC

34

PLF G ++AE+EI A ++CTG++T CC

2

PLFTGGQFAEAEIISEASSLTTFGGCGTACTGSHTRYCC

40

allorf_010170_010304

Leader:

MVGNADPGRPAGQCHRKQTAPFA

Core:

GVRVKRWCKRPPAPTVTWAAR

Core with dehydrations:

GVRVKRWCysKRPPAPDhbVDhbWAAR

antiSMASH-DB 4.0 matches for allorf_010170_010304

100.0%

NZ_VANP01000002.1:

Lanthipeptides: allorf_010170_010304_lanthipeptide

1

GVRVKRWCKRPPAPTVTWAAR

21

GVRVKRWCKRPPAPTVTWAAR

1

GVRVKRWCKRPPAPTVTWAAR

21

33.3%

NZ_BMUU01000006.1and 1 other:

NZ_BMUZ01000004.1 Lanthipeptides: allorf_264042_264134_lanthipeptide

Lanthipeptides: allorf_267084_267176_lanthipeptide

7

WCKRPPAPTVT

17

WC+R P TVT

5

WCRRAPGRTVT

15

23.3%

NZ_CP095742.1:

Lanthipeptides: allorf_0677509_0677667_lanthipeptide

1

GVRVKRWCKR-----PPAPTV

16

GVR+KR C + PPA +V

8

GVRLKRHCPQHSGPLPPATSV

28

with 1 more sequence matches

19.0%

NZ_CP061033.1:

Lanthipeptides: allorf_2073279_2073341_lanthipeptide

1

GVRVKRWCK

9

G+R++R+C

5

GIRIRRFCH

13

MIBiG 4.0 matches for allorf_010170_010304

7.8%

BGC0002120.3 (NQE92413.1):

Lanthipeptide: nocathioamide A

9

KRPPAP

14

RP AP

33

SRPAAP

38

allorf_020225_020401

Leader:

MATGRRRGRASRRDAGAGPRARSGPGTRRDETGA

Core:

RCFPPHRATLLMELGDSGRGKSIS

Core with dehydrations:

RCysFPPHRADhbLLMELGDDhaGRGKDhaIDha

antiSMASH-DB 4.0 matches for allorf_020225_020401

100.0%

NZ_VANP01000002.1:

Lanthipeptides: allorf_020225_020401_lanthipeptide

1

RCFPPHRATLLMELGDSGRGKSIS

24

RCFPPHRATLLMELGDSGRGKSIS

1

RCFPPHRATLLMELGDSGRGKSIS

24

88.5%

NZ_JABBWY010000002.1and 1 other:

NZ_JABBWZ010000011.1 Lanthipeptides: allorf_012175_012357_lanthipeptide

Lanthipeptides: allorf_683196_683378_lanthipeptide

1

RCFPPHRATLLMELGDSGRGKSIS

24

RCFPPHRA+LLMELGDSGRGKSIS

1

RCFPPHRASLLMELGDSGRGKSIS

24

73.1%

NZ_VYTZ01000002.1:

Lanthipeptides: allorf_519450_519632_lanthipeptide

1

RCFPPHRATLLMELGDSGRGKSIS

24

RCF HR TL +ELGDSGRGKSIS

1

RCFRRHRVTLSIELGDSGRGKSIS

24

MIBiG 4.0 matches for allorf_020225_020401

20.8%

BGC0002150.2 (MBO0654592.1):

Lasso peptide: triculamin

15

GDSGRGKSI

23

GD RGK +

7

GDGIRGKGV

15

allorf_020423_020557

Leader:

MPCAVTRHALPSGSRIAVEPAA

Core:

WTTECDGATTERSPLTSLQSGM

Core with dehydrations:

WDhbDhbECysDGADhbDhbERDhaPLDhbDhaLQDhaGM

antiSMASH-DB 4.0 matches for allorf_020423_020557

100.0%

NZ_VANP01000002.1:

Lanthipeptides: allorf_020423_020557_lanthipeptide

1

WTTECDGATTERSPLTSLQSGM

22

WTTECDGATTERSPLTSLQSGM

1

WTTECDGATTERSPLTSLQSGM

22

13.5%

NZ_JAENHX010000011.1:

Lanthipeptides: JIN86_RS20375_lanthipeptide

1

WTTECDGAT

9

W+ EC+G T

27

WSAECNGTT

35

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (12):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.97 out of 39.62

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.92 out of 25.75

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.50 out of 30.22

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.26 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.91 out of 27.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.08 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.08 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.16 out of 29.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.79 out of 35.63

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.63 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.06 out of 28.19

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.65 out of 32.93

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

FED44_RS05225 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 3355.6 Da

Molecular weight: 3357.8 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3375.8 Da N = 1; 3393.9 Da N = 2; 3411.9 Da N = 3; 3429.9 Da N = 4

FED44_RS05230 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 3484.7 Da

Molecular weight: 3486.9 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3504.9 Da N = 1; 3523.0 Da N = 2; 3541.0 Da N = 3; 3559.0 Da N = 4; 3577.0 Da N = 5

allorf_010170_010304 - Class II

Cleavage pHMM score: -6.60

RODEO score: 0

Monoisotopic mass: 2398.4 Da

Molecular weight: 2399.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2417.9 Da N = 1

allorf_020225_020401 - Class II

Cleavage pHMM score: -4.50

RODEO score: 0

Monoisotopic mass: 2555.3 Da

Molecular weight: 2556.9 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2575.0 Da N = 1; 2593.0 Da N = 2; 2611.0 Da N = 3

allorf_020423_020557 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 2226.0 Da

Molecular weight: 2227.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2245.4 Da N = 1; 2263.4 Da N = 2; 2281.5 Da N = 3; 2299.5 Da N = 4; 2317.5 Da N = 5; 2335.5 Da N = 6; 2353.5 Da N = 7

TFBS definitions

Regulator	Description

NZ_VANP01000002.1 - Region 2 - terpene

Download region GenBank file

Download region SVG

Location: 609,091 - 630,011 nt. (total: 20,921 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

FED44_RS07775: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

FED44_RS07810: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Ligases: synthase

FED44_RS07780: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.53	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.53	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.51	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.51	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000649.3	0.49	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000656.5	0.47	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000645.3	0.45	terpene	carotenoid	Halobacillus halophilus DSM 2266
BGC0000650.3	0.45	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000648.3	0.42	terpene	carotenoid	Myxococcus xanthus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.53	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.53	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.51	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.51	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000649.3	0.49	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000656.5	0.47	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000645.3	0.45	terpene	carotenoid	Halobacillus halophilus DSM 2266
BGC0000650.3	0.45	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000648.3	0.42	terpene	carotenoid	Myxococcus xanthus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene		PSPP	C30	acyclic	PP
triterpene		squalene	C30	acyclic	none
triterpene	C30 carotenoid	diapophytoene	C30	acyclic	none
tetraterpene	C40 carotenoid	phytoene	C40	acyclic	none
triterpene		hydroxysqualene	C30	acyclic	OH

Genes with terpene domains:

FED44_RS07805: PT_phytoene_like

Domain 1 (12 - 301): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → PSPP; FPP → squalene; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (9):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.97 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.71 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.82 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.51 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.82 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.29 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.10 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 16.96 out of 22.40

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.05 out of 23.43

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description

NZ_VANP01000025.1 - Region 1 - NRPS,NRPS-like,T1PKS,terpene-precursor

Download region GenBank file

Download region SVG

Location: 1 - 61,080 nt. (total: 61,080 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

FED44_RS34600: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

FED44_RS34620: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

FED44_RS34670: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

FED44_RS34680: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

FED44_RS34720: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

FED44_RS34725: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

FED44_RS34730: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

FED44_RS34790: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

Transferases: aminotransferase sulfhydrylase

FED44_RS34590: O-succinylhomoserine sulfhydrylase

smcogs: SMCOG1168: O-succinylhomoserine sulfhydrylase

FED44_RS34635: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

FED44_RS34750: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

FED44_RS34755: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: amidase deacetylase esterase

FED44_RS34615: thioesterase

smcogs: SMCOG1004: thioesterase

FED44_RS34640: thioesterase

smcogs: SMCOG1004: thioesterase

FED44_RS34645: thioesterase

smcogs: SMCOG1004: thioesterase

FED44_RS34715: amidase

smcogs: SMCOG1105: amidase

FED44_RS34780: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

Lyases: lyase

FED44_RS34630: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Isomerases: isomerase

FED44_RS34660: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase synthetase

FED44_RS34585: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS34605: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS34690: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

FED44_RS34695: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS34705: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

FED44_RS34735: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS34765: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

FED44_RS34800: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.88	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000357.5	0.85	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001166.5	0.84	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001268.4	0.75	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.75	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.67	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.67	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

FED44_RS34765: PT_FPPS_like

Domain 1 (51 - 347): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.18 out of 20.47

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 20.10 out of 21.84

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.33 out of 19.52

Confidence: strong

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 24.59 out of 27.18

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.49 out of 25.31

Confidence: medium

Additional weak hits (12):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.81 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.60 out of 27.78

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.90 out of 30.22

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.07 out of 28.14

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.25 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.67 out of 31.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.67 out of 21.55

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 20.25 out of 30.97

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.76 out of 27.04

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.52 out of 20.01

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.04 out of 32.74

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Terpene

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 61080:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (D-Tyr - Ile) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 33562:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (D-Tyr - Ile) + (mal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 0 - 22977:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val) + (D-Tyr - Ile)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 10988 - 54342:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - mal) + (D-Tyr - Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS34585: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (25..416): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDGCFDPSAFEGWMLGGGEVNGYGPTEDTCFTSC
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (68% 8Å match)
Stachelhaus code match:: 80% (moderate)

FED44_RS34605: Tyr - Ile

Search NORINE for peptide: strict or relaxed

AMP-binding (21..411): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LEQAFDASVSEVTLIVAGEINAYGPTETTVCATM
Nearest Stachelhaus code(s):: Tyr DASTIAAVCK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1546..1941): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LDPTGDSLAFEIWVWT-GDVNGYGSSETAVFTTR
Nearest Stachelhaus code(s):: Ile or aIle DAFWWGGVFK (59% 8Å match)
Stachelhaus code match:: 80% (moderate)

FED44_RS34690: mal

PKS_AT (5..284): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 80.9
Methoxymalonyl-CoA: 39.6
Methylmalonyl-CoA: 32.6
inactive: 19.1
Ethylmalonyl-CoA: 13.6
fatty_acid: 11.8
Isobutyryl-CoA: 11.0
Benzoyl-CoA: 10.5
trans-1,2-CPDA: 7.5
Propionyl-CoA: 1.2
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

FED44_RS34695: X

AMP-binding (15..416): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ATTTHTVGPMAAFWLYGGTVQTYGSTETWEIGTM
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

FED44_RS34735: X

AMP-binding (25..434): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RTMHWAFAQLGPEALNTFDGQSWGQSETGPVTLG
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CatR	H2O2-responsive repressor
MexT	Global virulence regulator
NrdR	Represses ribonucleotide reductase encoding genes
VqsM	Regulator of quorum-sensing signalling systems

NZ_VANP01000028.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 23,059 nt. (total: 23,059 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

FED44_RS37915: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase

FED44_RS38285: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001051.5	0.64	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.62	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.60	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001051.5	0.64	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002192.3	0.62	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.62	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.62	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.60	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 23059:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (ccmal - ccmal - ccmal)
Non-colinear ordering:: FED44_RS38285 + FED44_RS37915

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS38285: mal

PKS_AT (558..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 200.3
inactive: 97.7
Methoxymalonyl-CoA: 72.9
Methylmalonyl-CoA: 63.7
Isobutyryl-CoA: 41.1
Propionyl-CoA: 31.4
Ethylmalonyl-CoA: 30.5
2-Methylbutyryl-CoA: 27.1
Acetyl-CoA: 21.9
trans-1,2-CPDA: 17.3
Benzoyl-CoA: 16.7
fatty_acid: 14.8
3-Methylbutyryl-CoA: 2.8
CHC-CoA: 2.1

FED44_RS37915: mal - mal - mal

PKS_AT (561..849): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.5
inactive: 112.4
Methoxymalonyl-CoA: 62.9
Methylmalonyl-CoA: 60.8
Propionyl-CoA: 26.0
Acetyl-CoA: 24.1
Isobutyryl-CoA: 21.8
Ethylmalonyl-CoA: 21.5
Benzoyl-CoA: 20.4
fatty_acid: 20.0
trans-1,2-CPDA: 15.7
CHC-CoA: 12.8
3-Methylbutyryl-CoA: 3.3
2-Methylbutyryl-CoA: -2.1

PKS_AT (2311..2601): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.6
inactive: 103.8
Methoxymalonyl-CoA: 62.2
Methylmalonyl-CoA: 55.9
Ethylmalonyl-CoA: 24.1
Acetyl-CoA: 23.0
Propionyl-CoA: 21.7
Isobutyryl-CoA: 20.6
trans-1,2-CPDA: 19.4
fatty_acid: 18.7
Benzoyl-CoA: 18.2
CHC-CoA: 12.5
2-Methylbutyryl-CoA: 10.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (4076..4366): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.8
inactive: 99.1
Methoxymalonyl-CoA: 61.5
Methylmalonyl-CoA: 57.2
Acetyl-CoA: 23.0
Ethylmalonyl-CoA: 22.0
Propionyl-CoA: 21.5
Isobutyryl-CoA: 21.2
Benzoyl-CoA: 18.3
fatty_acid: 17.8
trans-1,2-CPDA: 16.9
2-Methylbutyryl-CoA: 9.9
CHC-CoA: 0.1
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

NZ_VANP01000032.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 6,013 nt. (total: 6,013 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acylase

FED44_RS35285: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: synthase

FED44_RS35280: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000041.3	0.62	PKS	oronofacic acid	Pseudomonas syringae
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.62	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000037.4	0.62	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001273.3	0.61	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.61	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.60	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.60	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002154.2	0.60	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000041.3	0.62	PKS	oronofacic acid	Pseudomonas syringae
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.62	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0000037.4	0.62	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0001273.3	0.61	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.61	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.60	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.60	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002154.2	0.60	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.60	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.52 out of 18.57

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 6013:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS35285: mmal

PKS_AT (764..1062): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.7
Ethylmalonyl-CoA: 206.6
Methoxymalonyl-CoA: 172.5
2-Methylbutyryl-CoA: 148.7
trans-1,2-CPDA: 128.2
Malonyl-CoA: 110.9
Propionyl-CoA: 101.2
CHC-CoA: 86.7
Isobutyryl-CoA: 82.6
Benzoyl-CoA: 63.5
inactive: 43.4
Acetyl-CoA: 39.3
3-Methylbutyryl-CoA: 30.4
fatty_acid: 30.3

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description

NZ_VANP01000037.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 2,973 nt. (total: 2,973 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

FED44_RS35370: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000342.4	0.62	NRPS	enniatin	Fusarium equiseti
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000357.5	0.62	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001240.4	0.62	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001166.5	0.62	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000342.4	0.62	NRPS	enniatin	Fusarium equiseti
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000357.5	0.62	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001240.4	0.62	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001166.5	0.62	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NZ_VANP01000004.1 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 25,336 nt. (total: 25,336 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: transferase

FED44_RS11325: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Ligases: synthase

FED44_RS37230: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.3	0.61	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.60	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.60	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.60	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.60	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001254.3	0.60	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.59	PKS	FR901512	fungal sp. No.14919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001160.5	0.61	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.61	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001273.3	0.61	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.60	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.60	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.60	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.60	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001254.3	0.60	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.59	PKS	FR901512	fungal sp. No.14919

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (27):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.83 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.46 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.04 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.66 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.03 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.97 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.04 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.00 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.12 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.06 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.18 out of 27.78

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.11 out of 30.22

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.24 out of 29.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 16.87 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.36 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.42 out of 29.38

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.87 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.40 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.52 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.54 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.17 out of 27.78

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.18 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.05 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.35 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.57 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.29 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 25336:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS37230: mmal

PKS_AT (587..815): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 62.5%; Malonyl-CoA: 58.3%; Ethylmalonyl-CoA: 58.3%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 77.6
Methylmalonyl-CoA: 71.8
Ethylmalonyl-CoA: 68.9
Malonyl-CoA: 62.8
Methoxymalonyl-CoA: 62.7
2-Methylbutyryl-CoA: 52.2
trans-1,2-CPDA: 39.6
Propionyl-CoA: 38.5
Benzoyl-CoA: 25.1
Isobutyryl-CoA: 20.7
inactive: 13.9
fatty_acid: 12.5
Acetyl-CoA: 12.2
3-Methylbutyryl-CoA: 7.9

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator

NZ_VANP01000004.1 - Region 2 - terpene

Download region GenBank file

Download region SVG

Location: 437,122 - 476,423 nt. (total: 39,302 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

FED44_RS13455: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

FED44_RS13515: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

FED44_RS13525: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Hydrolases: deacetylase hydrolase

FED44_RS13420: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

FED44_RS13555: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Ligases: synthetase

FED44_RS13520: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.56	terpene	carotenoid	Rhodobacter sphaeroides
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000663.5	0.53	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0002610.3	0.50	terpene	stellatic acid	Aspergillus stellatus
BGC0002162.3	0.50	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0000646.5	0.48	terpene	β-carotein	uncultured bacterium
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000664.5	0.47	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.47	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.47	terpene	carotenoid	Myxococcus xanthus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000647.3	0.56	terpene	carotenoid	Rhodobacter sphaeroides
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000663.5	0.53	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0002610.3	0.50	terpene	stellatic acid	Aspergillus stellatus
BGC0002162.3	0.50	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0000646.5	0.48	terpene	β-carotein	uncultured bacterium
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000664.5	0.47	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000633.3	0.47	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.47	terpene	carotenoid	Myxococcus xanthus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

FED44_RS13425: PT_phytoene_like

Domain 1 (10 - 254): Prenyltransferase; Phytoene synthase like

Subtype: Hydroxysqualene synthase

Reactions:: PSPP → hydroxysqualene

FED44_RS13430: PT_phytoene_like

Domain 1 (48 - 363): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → PSPP; FPP → squalene; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

FED44_RS13440: T2TS

Domain 1 (7 - 618): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

FED44_RS13520: PT_FPPS_like

Domain 1 (60 - 348): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (7):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.07 out of 33.73

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.73 out of 35.63

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.67 out of 20.01

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.14 out of 24.78

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.22 out of 25.88

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.04 out of 26.85

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description

NZ_VANP01000004.1 - Region 3 - azole-containing-RiPP,thioamitides

Download region GenBank file

Download region SVG

Location: 583,011 - 614,778 nt. (total: 31,768 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)
thioamitides: ((YcaO or TIGR03604) and TfuA)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

FED44_RS14130: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

FED44_RS38080: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

Transferases: methyltransferase

FED44_RS14125: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002099.3	0.48	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0002084.4	0.48	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0001849.4	0.46	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0001201.3	0.40	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0000844.5	0.37	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002560.3	0.35	ribosomal	thiostreptamide S4	Streptomyces sp. NRRL S-4
BGC0002127.3	0.34	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0001485.4	0.31	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0000902.4	0.31	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0001336.5	0.30	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002099.3	0.57	ribosomal	biarylitide YFH	Planomonospora sp. ID107089
BGC0002084.4	0.57	ribosomal	biarylitide YYH	Planomonospora sp. ID82291
BGC0001849.4	0.56	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0001201.3	0.52	terpene	eremophilene	Sorangium cellulosum So ce56
BGC0000844.5	0.50	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0002560.3	0.49	ribosomal	thiostreptamide S4	Streptomyces sp. NRRL S-4
BGC0002127.3	0.48	PKS	flaviolin, 1,3,6,8-tetrahydroxynaphthalene	Streptomyces coelicolor A3(2)
BGC0001485.4	0.46	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0000902.4	0.46	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0001336.5	0.45	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.15 out of 23.79

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.29 out of 27.78

Confidence: medium

Additional weak hits (7):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.86 out of 30.25

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.42 out of 39.62

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.77 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.63 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.14 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.06 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.17 out of 29.38

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

RREFinder

TFBS Finder

Azole RiPPs

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 1 - 89; Score: 45.9
: RRE type: Stand_Alone_Lasso_RRE; Location: 1 - 89; Score: 45.9

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
IolR	Regulator of the inositol catabolism

Core peptide predictions

No core peptides found.

NZ_VANP01000005.1 - Region 1 - T3PKS

Download region GenBank file

Download region SVG

Location: 107,805 - 148,875 nt. (total: 41,071 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

FED44_RS14795: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: synthase

FED44_RS14815: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

FED44_RS14825: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.58	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001164.3	0.55	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.55	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001831.3	0.45	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0002318.2	0.42	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0001294.5	0.41	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0000903.3	0.40	other (other)	fluorometabolite	Streptomyces cattleya
BGC0002387.2	0.39	PKS	yangpumicin A	Micromonospora yangpuensis
BGC0000205.3	0.39	PKS	bryostatin	Candidatus Endobugula sertula
BGC0001647.3	0.38	PKS	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.58	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001164.3	0.55	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.55	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001831.3	0.45	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0002318.2	0.42	ribosomal	canucin A, canucin B	Streptomyces canus
BGC0001294.5	0.41	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0000903.3	0.40	other (other)	fluorometabolite	Streptomyces cattleya
BGC0002387.2	0.39	PKS	yangpumicin A	Micromonospora yangpuensis
BGC0000205.3	0.39	PKS	bryostatin	Candidatus Endobugula sertula
BGC0001647.3	0.38	PKS	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.32 out of 23.79

Confidence: medium

Additional weak hits (18):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.90 out of 30.22

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.13 out of 30.97

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.20 out of 36.54

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.88 out of 31.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 21.27 out of 36.54

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 22.07 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.74 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.32 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.82 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.79 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.19 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.21 out of 29.38

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.40 out of 18.57

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.27 out of 30.22

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.55 out of 32.93

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
MexT	Global virulence regulator

NZ_VANP01000005.1 - Region 2 - NRPS,lanthipeptide-class-iv

Download region GenBank file

Download region SVG

Location: 157,507 - 228,799 nt. (total: 71,293 nt) Show pHMM detection rules used

lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

FED44_RS14975: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

FED44_RS14980: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

FED44_RS15120: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: methyltransferase

FED44_RS14925: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase

FED44_RS14920: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: ligase synthase

FED44_RS15015: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS15020: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS15025: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

FED44_RS15035: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

FED44_RS15045: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

FED44_RS15050: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS15090: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

FED44_RS37325: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: transporter

FED44_RS14990: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.46	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.44	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.44	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.43	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.42	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.42	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.41	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.40	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.39	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001050.5	0.39	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.74	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.71	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001050.5	0.71	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 30.11 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 30.11 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.15 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 29.02 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 28.65 out of 30.11

Confidence: strong

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 21.08 out of 23.43

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 25.39 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 25.19 out of 30.11

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.08 out of 18.57

Confidence: medium

Additional weak hits (33):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.53 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.72 out of 28.14

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.29 out of 25.75

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.48 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.04 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.83 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.21 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.46 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 19.08 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.30 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.56 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.15 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.80 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.36 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.27 out of 29.25

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.64 out of 21.55

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.84 out of 30.22

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.67 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.85 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.96 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.76 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.92 out of 26.06

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.91 out of 39.62

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.92 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.62 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.59 out of 36.54

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.39 out of 30.22

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.01 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.28 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.97 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.37 out of 28.19

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.57 out of 24.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.48 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

No core peptides found.

Predicted core structure(s)

For candidate cluster 2, location 157506 - 228799:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Gly) + (Asp)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 170447 - 228799:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Gly) + (Asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS15015: X

AMP-binding (460..870): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RWMAFDTHLEEPDLIIGGELNTYGPTEAAVIATW
Nearest Stachelhaus code(s):: Lys DTEDIGSVIK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)

FED44_RS15020: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (29..429): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: LSFTFDISVLELQALCGGEWNVYGPTETTVWSTA
Nearest Stachelhaus code(s):: Gly DILQLGVVWK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

FED44_RS15050: X

AMP-binding (21..416): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: CQPFTNAGQTMNFLGSAAATNYYTSTEAAPAQTI
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

FED44_RS37325: Asp

Search NORINE for peptide: strict or relaxed

AMP-binding (552..965): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YGVQFDFGVTVLKILLGGEVNHYGPTEATVGVTT
Nearest Stachelhaus code(s):: Asp DLTKIGHVGK (74% 8Å match); Asn DLTKLGEVGK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
BldD	Development and antibiotic global regulator
CelR	Cellobiose uptake repressor
ToxT	Regulator of MSHA biosynthesis

NZ_VANP01000005.1 - Region 3 - T3PKS,lanthipeptide-class-iii

Download region GenBank file

Download region SVG

Location: 446,707 - 487,786 nt. (total: 41,080 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

FED44_RS16120: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

FED44_RS16125: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

FED44_RS16185: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

FED44_RS16235: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

Transferases: aminotransferase methyltransferase

FED44_RS16230: Methylation

MITE: MITE0000155Methylation (identity: 70.20%)
smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

FED44_RS16300: aminotransferase

smcogs: SMCOG1019: aminotransferase

Ligases: ligase synthase

FED44_RS16200: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

FED44_RS16225: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

FED44_RS16285: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	1.14	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.64	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000513.3	0.63	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000496.5	0.59	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000501.3	0.54	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.54	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0001831.3	0.45	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0000518.3	0.45	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000553.3	0.42	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0002700.2	0.38	ribosomal	bacinapeptin	Bacillus nakamurai

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.83	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000513.3	0.65	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000496.5	0.63	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0002276.2	0.58	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000518.3	0.56	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0001831.3	0.56	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0000553.3	0.53	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for FED44_RS16245

FED44_RS16250

Leader:

MALLDLQTLETPGGHGGGGGSN

Core:

LSLLACTPHENSSLSLICG

Core with dehydrations:

LDhaLLACysDhbPHENDhaDhaLDhaLICysG

antiSMASH-DB 4.0 matches for FED44_RS16250

86.4%

NZ_BMMN01000011.1and 5 others:

NZ_FTNI01000016.1 Lanthipeptides: BXA11_RS42315_lanthipeptideNZ_JABBWY010000006.1 Lanthipeptides: HHX37_RS18530_lanthipeptideNZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS12960_lanthipeptideNZ_VANP01000005.1 Lanthipeptides: FED44_RS16250_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17725_lanthipeptide

Lanthipeptides: IEY80_RS26005_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLLACTPHENSSLSLICG

4

LSLLACTPHENSSLSLICG

22

85.0%

NZ_JABBWV010000002.1:

Lanthipeptides: HHX31_RS05740_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLSL+CG

2

LSLLGCTPHENSSLSLVCG

20

80.0%

NZ_JABBWU010000019.1:

Lanthipeptides: HHX30_RS29855_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

+SLL CTPHENSSLS+ICG

2

VSLLGCTPHENSSLSIICG

20

with 16 more sequence matches

Only the first 10 are displayed here.

80.0%

NZ_JABBWX010000013.1:

Lanthipeptides: HHX35_RS30140_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLS++CG

2

LSLLGCTPHENSSLSIVCG

20

77.3%

NZ_BCBX01000043.1and 1 other:

NZ_BCBX01000043.1 Lanthipeptides: AP234_RS36585_lanthipeptide

Lanthipeptides: AP234_RS36580_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLLAC PHENSSLSL+CG

4

LSLLACVPHENSSLSLLCG

22

77.3%

NZ_BMMN01000011.1and 6 others:

NZ_FTNI01000016.1 Lanthipeptides: BXA11_RS42310_lanthipeptideNZ_JABBWY010000006.1 Lanthipeptides: HHX37_RS18535_lanthipeptideNZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS12965_lanthipeptideNZ_VANP01000005.1 Lanthipeptides: FED44_RS16255_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17720_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17715_lanthipeptide

Lanthipeptides: IEY80_RS26010_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLS+ICG

4

LSLLGCTPHENSSLSIICG

22

75.0%

NZ_JABBWU010000019.1and 6 others:

NZ_JABBWU010000019.1 Lanthipeptides: HHX30_RS29850_lanthipeptideNZ_JABBWV010000002.1 Lanthipeptides: HHX31_RS05735_lanthipeptideNZ_JABBWX010000013.1 Lanthipeptides: HHX35_RS30145_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08060_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08055_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08065_lanthipeptide

Lanthipeptides: HHX30_RS29860_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLSLICG

6

LSLLGCTPHENSSLSLICG

24

69.6%

NZ_KI866522.1:

Lanthipeptides: MPTA5024_RS40525_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLS++CG

4

LSLLGCTPHENSSLSVLCG

22

69.6%

NZ_QOIL01000022.1and 1 other:

NZ_QOIL01000022.1 Lanthipeptides: DQ384_RS31185_lanthipeptide

Lanthipeptides: DQ384_RS31190_lanthipeptide

1

LSLLACTPHENSSLSLICG

19

LSLL CTPHENSSLS++CG

4

LSLLGCTPHENSSLSILCG

22

50.0%

NZ_AUBE01000003.1:

Lanthipeptides: G412_RS38950_lanthipeptide

1

LSLLACTPHENSSLSLIC

18

LSLLAC H ++S L+C

3

LSLLACDKHSSNSF-LLC

19

45.5%

NZ_CM000913.1and 1 other:

NZ_CP016559.1 Lanthipeptides: BB341_RS04185_lanthipeptide

Lanthipeptides: SCLAV_RS23790_lanthipeptide

2

SLLACTPHENSSLSLIC

18

SLL C PH + SL L+C

6

SLLVCGPHSSQSL-LLC

21

42.9%

NZ_CP042266.1:

Lanthipeptides: FQU76_RS00910_lanthipeptide

1

LSLLACTPHENSSL

14

LSLL+C H N SL

5

LSLLSCGHHSNLSL

18

38.1%

NZ_BMQJ01000002.1:

Lanthipeptides: IEZ10_RS04315_lanthipeptide

1

LSLLACTPHENSSLSLI

17

L++L C H S+LSL+

2

LTVLGCESHTPSNLSLL

18

FED44_RS16255

Leader:

MALLDLQTLEAPTGGGGG

Core:

GTQGSNLSLLGCTPHENSSLSIICG

Core with dehydrations:

GDhbQGDhaNLDhaLLGCysDhbPHENDhaDhaLDhaIICysG

antiSMASH-DB 4.0 matches for FED44_RS16255

100.0%

NZ_JABBWY010000006.1and 2 others:

NZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS12965_lanthipeptideNZ_VANP01000005.1 Lanthipeptides: FED44_RS16255_lanthipeptide

Lanthipeptides: HHX37_RS18535_lanthipeptide

1

GTQGSNLSLLGCTPHENSSLSIICG

25

GTQGSNLSLLGCTPHENSSLSIICG

1

GTQGSNLSLLGCTPHENSSLSIICG

25

88.0%

NZ_BMMN01000011.1and 3 others:

NZ_FTNI01000016.1 Lanthipeptides: BXA11_RS42310_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17720_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17715_lanthipeptide

Lanthipeptides: IEY80_RS26010_lanthipeptide

4

GSNLSLLGCTPHENSSLSIICG

25

GSNLSLLGCTPHENSSLSIICG

1

GSNLSLLGCTPHENSSLSIICG

22

84.0%

NZ_JABBWU010000019.1and 5 others:

NZ_JABBWU010000019.1 Lanthipeptides: HHX30_RS29850_lanthipeptideNZ_JABBWV010000002.1 Lanthipeptides: HHX31_RS05735_lanthipeptideNZ_JABBWX010000013.1 Lanthipeptides: HHX35_RS30145_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08060_lanthipeptideNZ_JABBXA010000003.1 Lanthipeptides: HHX41_RS08055_lanthipeptide

Lanthipeptides: HHX30_RS29860_lanthipeptide

4

GSNLSLLGCTPHENSSLSIICG

25

GSNLSLLGCTPHENSSLS+ICG

3

GSNLSLLGCTPHENSSLSLICG

24

with 30 more sequence matches

Only the first 10 are displayed here.

84.0%

NZ_QOIL01000022.1and 1 other:

NZ_QOIL01000022.1 Lanthipeptides: DQ384_RS31185_lanthipeptide

Lanthipeptides: DQ384_RS31190_lanthipeptide

4

GSNLSLLGCTPHENSSLSIICG

25

GSNLSLLGCTPHENSSLSI+CG

1

GSNLSLLGCTPHENSSLSILCG

22

80.0%

NZ_BMMN01000011.1and 1 other:

NZ_FTNI01000016.1 Lanthipeptides: BXA11_RS42315_lanthipeptide

Lanthipeptides: IEY80_RS26005_lanthipeptide

4

GSNLSLLGCTPHENSSLSIICG

25

GSNLSLL CTPHENSSLS+ICG

1

GSNLSLLACTPHENSSLSLICG

22

80.0%

NZ_KI866522.1:

Lanthipeptides: MPTA5024_RS40525_lanthipeptide

4

GSNLSLLGCTPHENSSLSIICG

25

GSNLSLLGCTPHENSSLS++CG

1

GSNLSLLGCTPHENSSLSVLCG

22

76.0%

NZ_JABBWU010000019.1:

Lanthipeptides: HHX30_RS29855_lanthipeptide

6

NLSLLGCTPHENSSLSIICG

25

N+SLLGCTPHENSSLSIICG

1

NVSLLGCTPHENSSLSIICG

20

76.0%

NZ_JABBWX010000013.1:

Lanthipeptides: HHX35_RS30140_lanthipeptide

6

NLSLLGCTPHENSSLSIICG

25

NLSLLGCTPHENSSLSI+CG

1

NLSLLGCTPHENSSLSIVCG

20

76.0%

NZ_JABBXA010000003.1:

Lanthipeptides: HHX41_RS08065_lanthipeptide

6

NLSLLGCTPHENSSLSIICG

25

NLSLLGCTPHENSSLS+ICG

1

NLSLLGCTPHENSSLSLICG

20

72.0%

NZ_BCBX01000043.1and 1 other:

NZ_BCBX01000043.1 Lanthipeptides: AP234_RS36585_lanthipeptide

Lanthipeptides: AP234_RS36580_lanthipeptide

4

GSNLSLLGCTPHENSSLSIICG

25

GSNLSLL C PHENSSLS++CG

1

GSNLSLLACVPHENSSLSLLCG

22

72.0%

NZ_JABBWV010000002.1:

Lanthipeptides: HHX31_RS05740_lanthipeptide

6

NLSLLGCTPHENSSLSIICG

25

NLSLLGCTPHENSSLS++CG

1

NLSLLGCTPHENSSLSLVCG

20

68.0%

NZ_JABBWY010000006.1and 3 others:

NZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS12960_lanthipeptideNZ_VANP01000005.1 Lanthipeptides: FED44_RS16250_lanthipeptideNZ_VYTZ01000006.1 Lanthipeptides: F5972_RS17725_lanthipeptide

Lanthipeptides: HHX37_RS18530_lanthipeptide

7

LSLLGCTPHENSSLSIICG

25

LSLL CTPHENSSLS+ICG

1

LSLLACTPHENSSLSLICG

19

48.0%

NZ_BIFH01000016.1:

Lanthipeptides: EMA17_RS12985_lanthipeptide

4

GSNLSLLGCTPHENSSLSII

23

GSN+SLL C NSSLS++

3

GSNVSLLLCG---NSSLSLL

19

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.24 out of 19.26

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.79 out of 25.31

Confidence: medium

Additional weak hits (30):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.49 out of 30.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.24 out of 27.78

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.80 out of 19.26

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.82 out of 26.33

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.12 out of 30.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.22 out of 29.25

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.35 out of 25.88

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.63 out of 18.57

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.12 out of 21.55

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.11 out of 25.75

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.14 out of 18.57

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.13 out of 30.97

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.25 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.43 out of 36.54

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.62 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.50 out of 21.55

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.57 out of 27.55

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.29 out of 35.63

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.04 out of 35.63

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.60 out of 30.98

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.53 out of 24.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.04 out of 18.57

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.52 out of 29.25

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.20 out of 33.14

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.95 out of 35.63

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.98 out of 29.65

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.06 out of 26.06

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.52 out of 27.04

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

FED44_RS16250 - Class III

Cleavage pHMM score: 15.70

RODEO score: 0

Monoisotopic mass: 1867.0 Da

Molecular weight: 1868.2 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1886.2 Da N = 1; 1904.2 Da N = 2; 1922.2 Da N = 3

FED44_RS16255 - Class III

Cleavage pHMM score: 10.30

RODEO score: 0

Monoisotopic mass: 2361.2 Da

Molecular weight: 2362.6 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2380.6 Da N = 1; 2398.6 Da N = 2; 2416.7 Da N = 3; 2434.7 Da N = 4; 2452.7 Da N = 5

NRPS/PKS substrate predictions

FED44_RS16285: X

AMP-binding (23..482): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RALAYIEGQQY-SVWCATAIDVYGMTETTGAFTV
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor

NZ_VANP01000006.1 - Region 1 - NRPS-like,T1PKS,phenazine

Download region GenBank file

Download region SVG

Location: 166,989 - 229,478 nt. (total: 62,490 nt) Show pHMM detection rules used

phenazine: phzB
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

FED44_RS17600: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

FED44_RS17635: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

FED44_RS17750: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: dehalogenase hydrolase

FED44_RS17520: isochorismatase

smcogs: SMCOG1027: isochorismatase

FED44_RS17680: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

FED44_RS17715: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Lyases: hydratase

FED44_RS17630: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Isomerases: isomerase

FED44_RS17515: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

FED44_RS17670: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS17675: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.76	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.62	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002164.2	0.62	NRPS	peramine	Epichloe festucae
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0001273.3	0.60	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.59	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.59	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.59	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001276.3	0.59	PKS	6-methylsalicyclic acid	Aspergillus terreus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000936.5	0.71	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0001249.5	0.70	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0002164.2	0.64	NRPS	peramine	Epichloe festucae
BGC0001261.3	0.64	NRPS	AM-toxin	Alternaria alternata
BGC0001273.3	0.64	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.37 out of 20.47

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.27 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.43 out of 23.79

Confidence: medium

Additional weak hits (18):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.95 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.60 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.00 out of 29.38

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.03 out of 21.55

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.93 out of 28.14

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.56 out of 21.55

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.58 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.74 out of 29.38

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.48 out of 26.85

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.16 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.48 out of 29.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.59 out of 35.63

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.09 out of 24.78

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 22.12 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.49 out of 28.19

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.59 out of 23.43

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.37 out of 28.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.09 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 166988 - 229478:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 182988 - 224878:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 184918 - 229478:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS17670: Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (13..414): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LALAFDLSVVEWFVILGGEYNAYGPTEATVATTV
Nearest Stachelhaus code(s):: Hty DLGFTGAVCK (65% 8Å match); OH-Asp or OMe-Asp DLTKIGAVNK (56% 8Å match); Asp DLTKIGAVNK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

FED44_RS17675: mal

PKS_AT (560..846): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Propionyl-CoA: 54.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 101.0
Methoxymalonyl-CoA: 68.6
Methylmalonyl-CoA: 63.3
inactive: 54.2
Ethylmalonyl-CoA: 40.1
Isobutyryl-CoA: 39.0
Acetyl-CoA: 29.8
Propionyl-CoA: 29.3
fatty_acid: 23.2
Benzoyl-CoA: 19.6
CHC-CoA: 17.1
trans-1,2-CPDA: 13.2
2-Methylbutyryl-CoA: 11.1
3-Methylbutyryl-CoA: 9.1

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_VANP01000007.1 - Region 1 - NRPS-like

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Location: 110,104 - 152,860 nt. (total: 42,757 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

FED44_RS19755: dioxygenase, TauD/TfdA

smcogs: SMCOG1121: dioxygenase, TauD/TfdA

Transferases: aminotransferase formyltransferase

FED44_RS19705: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

FED44_RS19725: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Isomerases: mutase

FED44_RS19635: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase

FED44_RS19695: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.60	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.59	NRPS	AM-toxin	Alternaria alternata
BGC0001817.5	0.46	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000410.5	0.44	NRPS	pseudomonine	Pseudomonas fluorescens
BGC0001843.2	0.44	NRPS	tridecaptin M	Paenibacillus sp. M-152
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000449.5	0.42	NRPS	tridecaptin	Paenibacillus terrae
BGC0001292.5	0.42	other (other)	A33853	Streptomyces sp. NRRL 12068

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.60	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.59	NRPS	AM-toxin	Alternaria alternata
BGC0001817.5	0.46	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0000410.5	0.44	NRPS	pseudomonine	Pseudomonas fluorescens
BGC0001843.2	0.44	NRPS	tridecaptin M	Paenibacillus sp. M-152
BGC0002150.2	0.43	ribosomal	triculamin	Streptomyces triculaminicus
BGC0002151.2	0.43	ribosomal	alboverticillin	Streptomyces griseocarneus
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000449.5	0.42	NRPS	tridecaptin	Paenibacillus terrae
BGC0001292.5	0.42	other (other)	A33853	Streptomyces sp. NRRL 12068

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.31 out of 18.46

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.26 out of 23.79

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 25.84 out of 30.12

Confidence: medium

Additional weak hits (14):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.18 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.32 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.93 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.54 out of 27.78

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.97 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.93 out of 29.38

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.71 out of 31.73

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.24 out of 29.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.49 out of 29.38

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.56 out of 21.55

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.34 out of 27.18

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.86 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.61 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.18 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 110103 - 152860:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Tyr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS19695: Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (9..386): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAQSFDASISEATLVFAGELNAYGPTEGTVCFSI
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
OsdR	Development and stress management regulator
RpoN	Regulator of nitrogen assimilation and virulence

NZ_VANP01000007.1 - Region 2 - lanthipeptide-class-i

Download region GenBank file

Download region SVG

Location: 195,762 - 222,187 nt. (total: 26,426 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

FED44_RS19985: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

FED44_RS19990: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000544.4	0.41	ribosomal	planosporicin	Planomonospora alba
BGC0000514.5	0.36	ribosomal	gallidermin	Staphylococcus gallinarum
BGC0000529.5	0.34	ribosomal	microbisporicin A2	Microbispora corallina
BGC0000796.3	0.25	saccharide	S-layer glycan	Paenibacillus alvei

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000544.4	0.41	ribosomal	planosporicin	Planomonospora alba
BGC0000514.5	0.36	ribosomal	gallidermin	Staphylococcus gallinarum
BGC0000529.5	0.34	ribosomal	microbisporicin A2	Microbispora corallina
BGC0000796.3	0.25	saccharide	S-layer glycan	Paenibacillus alvei

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for FED44_RS19985

FED44_RS19980

Leader:

MSSNLGPVQGHPSGSPALGAAARRGPFDLDVSVVEAADAGSL

Core:

ISVTDDGCGTTCPKACTTGSR

Core with dehydrations:

IDhaVDhbDDGCysGDhbDhbCysPKACysDhbDhbGDhaR

antiSMASH-DB 4.0 matches for FED44_RS19980

100.0%

NZ_VANP01000007.1:

Lanthipeptides: FED44_RS19980_lanthipeptide

1

ISVTDDGCGTTCPKACTTGSR

21

ISVTDDGCGTTCPKACTTGSR

1

ISVTDDGCGTTCPKACTTGSR

21

63.6%

NZ_CP028834.1and 1 other:

NZ_CP074380.1 Lanthipeptides: KGS77_RS25025_lanthipeptide

Lanthipeptides: DBP13_RS26755_lanthipeptide

1

ISVTDDGCGTTCPKACTT

18

IS+TDDGCG TC ACTT

3

ISLTDDGCGETC-GACTT

19

61.9%

NZ_BBXD01000026.1and 1 other:

NZ_KB913036.1 Lanthipeptides: A39M_RS1000000128430_lanthipeptide

Lanthipeptides: AW272_RS36470_lanthipeptide

1

ISVTDDGCGTTCPKACTTG

19

+ +TDDGCG+TC ACTTG

1

VRLTDDGCGSTCT-ACTTG

18

with 166 more sequence matches

Only the first 10 are displayed here.

61.9%

NZ_BNBY01000025.1:

Lanthipeptides: IE332_RS31285_lanthipeptide

3

VTDDGCGTTCPKACTTGSR

21

+TDDGCG+TC ACTTG

2

LTDDGCGSTC-GACTTGFH

19

61.9%

NZ_CP023701.1:

Lanthipeptides: CP968_RS31915_lanthipeptide

2

SVTDDGCGTTCPKACTTG

19

++TDDGCG+TC ACTTG

1

NLTDDGCGSTC-GACTTG

17

61.9%

NZ_CP026498.1and 1 other:

NZ_KK853997.1 Lanthipeptides: KCH_RS39425_lanthipeptide

Lanthipeptides: C2142_RS04990_lanthipeptide

3

VTDDGCGTTCPKACTTGSR

21

+TDDGCG+TC ACTTG

2

LTDDGCGSTC-GACTTGVH

19

61.9%

NZ_FZOF01000021.1:

Lanthipeptides: CHF15_RS30750_lanthipeptide

3

VTDDGCGTTCPKACTTGS

20

+TDDGCG+TC ACTTG+

1

LTDDGCGSTC-GACTTGA

17

61.9%

NZ_JAINUL010000001.1:

Lanthipeptides: K7B10_RS07615_lanthipeptide

2

SVTDDGCGTTCPKACTTG

19

++TDDGCG+TC ACTTG

2

TLTDDGCGSTCG-ACTTG

18

61.9%

NZ_QQVZ01000001.1:

Lanthipeptides: DV517_RS13190_lanthipeptide

3

VTDDGCGTTCPKACTTG

19

+TDDGCG+TC ACTTG

2

LTDDGCGSTC-GACTTG

17

59.1%

NZ_AZXF01000017.1:

Lanthipeptides: B042_RS24865_lanthipeptide

1

ISVTDDGCGTTCPKACTT

18

I++TDDGCGTTCP C T

1

INLTDDGCGTTCPSTCVT

18

59.1%

NZ_BMXL01000030.1and 1 other:

NZ_CP099837.1 Lanthipeptides: NE857_RS26275_lanthipeptide

Lanthipeptides: IE143_RS19675_lanthipeptide

1

ISVTDDGCGTTCPKACTT

18

I++TDDGCG++CP ACTT

1

INLTDDGCGSSCPSACTT

18

59.1%

NZ_NDXL01000002.1:

Lanthipeptides: B7024_RS28290_lanthipeptide

1

ISVTDDGCGTTCPKACTT

18

IS+T+DGCG TC ACTT

3

ISLTNDGCGETC-GACTT

19

57.1%

NZ_AP018365.1:

Lanthipeptides: RVR_RS08440_lanthipeptide

2

SVTDDGCGTTCPKACTT

18

S+TDD CG+TC ACTT

2

SLTDDNCGSTC-GACTT

17

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.14 out of 23.79

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.75 out of 23.05

Confidence: medium

Additional weak hits (14):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.96 out of 26.33

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.96 out of 29.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.77 out of 29.38

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.78 out of 39.62

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.88 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.75 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.67 out of 26.06

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.21 out of 26.33

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.26 out of 30.22

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.72 out of 30.22

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.88 out of 20.97

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.07 out of 27.18

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.20 out of 27.18

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.72 out of 30.98

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

FED44_RS19980 - Class I

Cleavage pHMM score: -0.40

RODEO score: 22

Monoisotopic mass: 1945.9 Da

Molecular weight: 1947.1 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 1965.2 Da N = 1; 1983.2 Da N = 2; 2001.2 Da N = 3; 2019.2 Da N = 4

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 160 - 251; Score: 60.2

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
IolR	Regulator of the inositol catabolism

NZ_VANP01000007.1 - Region 3 - T1PKS

Download region GenBank file

Download region SVG

Location: 278,291 - 326,162 nt. (total: 47,872 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

FED44_RS20335: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: dehalogenase

FED44_RS20350: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

FED44_RS20370: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Lyases: dehydratase

FED44_RS20375: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

FED44_RS20380: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS20385: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

FED44_RS20390: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002276.2	0.64	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.62	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001268.4	0.62	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.66	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.66	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.66	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002276.2	0.64	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002191.2	0.62	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001268.4	0.62	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.61	PKS	ACT-Toxin II	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.28 out of 26.33

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.67 out of 30.11

Confidence: medium

Additional weak hits (11):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.80 out of 31.73

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.58 out of 30.11

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.31 out of 26.85

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.64 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.81 out of 21.84

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.57 out of 26.12

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 19.98 out of 27.18

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.73 out of 28.19

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.60 out of 30.11

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.10 out of 30.11

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.92 out of 18.57

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 278290 - 326162:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS20380: X

AMP-binding (24..447): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QLLA-------GFAVSAGALSVYGATECMPVAAI
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

FED44_RS20390: pk

PKS_AT (587..881): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; Methylmalonyl-CoA: 70.8%; CE-Malonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 114.5
Malonyl-CoA: 97.2
Methoxymalonyl-CoA: 94.7
Isobutyryl-CoA: 75.0
Ethylmalonyl-CoA: 71.5
CHC-CoA: 61.6
Propionyl-CoA: 47.2
trans-1,2-CPDA: 45.2
2-Methylbutyryl-CoA: 39.6
Benzoyl-CoA: 35.5
fatty_acid: 25.8
inactive: 23.5
Acetyl-CoA: 12.2
3-Methylbutyryl-CoA: 7.8

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CRP	Cyclic AMP receptor protein
CelR	Cellobiose uptake repressor

NZ_VANP01000008.1 - Region 1 - NRPS-like,PKS-like,blactam,butyrolactone,t3nrps-iterative

Download region GenBank file

Download region SVG

Location: 180,050 - 260,915 nt. (total: 80,866 nt) Show pHMM detection rules used

t3nrps-iterative: (AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC) and not ATP-grasp)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
butyrolactone: AfsA
PKS-like: (t2pks2 or ksIII)
blactam: (BLS or CAS or tabtoxin)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

FED44_RS21725: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

FED44_RS21740: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

FED44_RS21780: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

FED44_RS21795: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

FED44_RS21800: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

FED44_RS21810: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

FED44_RS21830: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

FED44_RS21885: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

FED44_RS21930: dioxygenase, TauD/TfdA

smcogs: SMCOG1121: dioxygenase, TauD/TfdA

FED44_RS21955: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: aminotransferase

FED44_RS21895: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: hydrolase

FED44_RS21935: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Lyases: decarboxylase hydratase lyase

FED44_RS21825: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

FED44_RS21905: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

FED44_RS21920: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

FED44_RS21940: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Ligases: ligase synthase

FED44_RS21755: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS21820: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

FED44_RS21850: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

FED44_RS21855: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000848.5	1.37	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000849.4	1.10	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0002276.2	1.09	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000850.5	0.94	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0000846.5	0.83	other (other)	tabtoxin	Pseudomonas syringae
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002578.2	0.71	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0000894.4	0.68	other (other)	citrinin	Monascus aurantiacus
BGC0001909.3	0.63	PKS	strobilurin A	Strobilurus tenacellus
BGC0002631.2	0.62	ribosomal	Myxarylin	Pyxidicoccus fallax

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000848.5	0.76	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002276.2	0.73	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000849.4	0.69	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000850.5	0.65	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0000846.5	0.60	other (other)	tabtoxin	Pseudomonas syringae
BGC0001135.5	0.59	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002578.2	0.58	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0000662.5	0.55	terpene	grixazone A	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002826.2	0.55	other (other)	closoxazole A, closoxazole B	Clostridium cavendishii DSM 21758
BGC0002631.2	0.53	ribosomal	Myxarylin	Pyxidicoccus fallax

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.04 out of 18.57

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.08 out of 18.57

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.52 out of 19.26

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

Additional weak hits (34):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.12 out of 29.38

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.45 out of 36.54

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.36 out of 18.57

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.50 out of 30.25

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.93 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.96 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.86 out of 26.06

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.09 out of 21.55

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.10 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.31 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.41 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 18.96 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.28 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 20.15 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.00 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.18 out of 31.73

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.41 out of 29.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.23 out of 30.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.20 out of 29.25

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.18 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.18 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.80 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.62 out of 31.73

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.51 out of 20.97

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.66 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.11 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.33 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.61 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.14 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.47 out of 28.14

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.33 out of 25.75

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.46 out of 33.14

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.14 out of 35.63

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.74 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 180049 - 260915:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS21755: X

AMP-binding (89..509): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MPPLHLVARRLKAMVLGGAFDSYGTTETGLVMLD
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

FED44_RS21820: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (50..453): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: PHLSWGKHAWSACAVAAGERDGYGQTETTAQIGT
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
ToxT	Regulator of MSHA biosynthesis

NZ_VANP01000008.1 - Region 2 - RiPP-like

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Location: 267,364 - 278,509 nt. (total: 11,146 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase

FED44_RS22040: methyltransferase

smcogs: SMCOG1089: methyltransferase

FED44_RS22050: methyltransferase

smcogs: SMCOG1248: methyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001157.6	0.35	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0000468.5	0.35	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0000470.6	0.35	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0000469.6	0.33	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0000249.5	0.29	PKS	nogalamycin	Streptomyces nogalater
BGC0002019.3	0.26	terpene	tiancilactone A, tiancilactone B, tiancilactone C, tiancilactone D, tiancilactone E, tiancilactone F, tiancilactone G, tiancilactone H	Streptomyces sp. CB03234
BGC0001484.3	0.24	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0002688.2	0.24	NRPS	benarthin, dibenarthin	Planobispora rosea
BGC0000907.5	0.22	other (other)	lincomycin	Streptomyces lincolnensis
BGC0001117.5	0.22	NRPS	himastatin	Streptomyces himastatinicus ATCC 53653

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001157.6	0.35	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0000468.5	0.35	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0000470.6	0.35	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0000469.6	0.33	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0000249.5	0.29	PKS	nogalamycin	Streptomyces nogalater
BGC0002019.3	0.26	terpene	tiancilactone A, tiancilactone B, tiancilactone C, tiancilactone D, tiancilactone E, tiancilactone F, tiancilactone G, tiancilactone H	Streptomyces sp. CB03234
BGC0001484.3	0.24	other (other)	2'-chloropentostatin, 2'-amino-2'-deoxyadenosine	Actinomadura sp. ATCC 39365
BGC0002688.2	0.24	NRPS	benarthin, dibenarthin	Planobispora rosea
BGC0000907.5	0.22	other (other)	lincomycin	Streptomyces lincolnensis
BGC0001117.5	0.22	NRPS	himastatin	Streptomyces himastatinicus ATCC 53653

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.00 out of 27.55

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.46 out of 31.73

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.78 out of 27.18

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.18 out of 27.18

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_VANP01000008.1 - Region 3 - terpene

Download region GenBank file

Download region SVG

Location: 288,710 - 311,180 nt. (total: 22,471 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

FED44_RS22170: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

FED44_RS22185: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Translocases: transporter

FED44_RS22135: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001910.4	0.51	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.51	terpene	raimonol	Streptomyces anulatus
BGC0000286.3	0.46	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001197.5	0.43	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001483.4	0.42	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0000678.5	0.41	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001840.2	0.39	NRPS, PKS	detoxin S1	Streptomyces sp. NRRL S-325
BGC0002339.3	0.39	terpene	2-hydroxysorangiadenosine	Vitiosangium cumulatum
BGC0002805.2	0.36	other (other)	Azodyrecin A, Azodyrecin B, Azodyrecin C	Streptomyces mirabilis strain P8-A
BGC0000188.5	0.36	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001910.4	0.51	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.51	terpene	raimonol	Streptomyces anulatus
BGC0000286.3	0.46	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001197.5	0.43	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001483.4	0.42	other (other)	5-isoprenylindole-3-carboxylate β-D-glycosyl ester	Streptomyces sp. RM-5-8
BGC0000678.5	0.41	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001840.2	0.39	NRPS, PKS	detoxin S1	Streptomyces sp. NRRL S-325
BGC0002339.3	0.39	terpene	2-hydroxysorangiadenosine	Vitiosangium cumulatum
BGC0002805.2	0.36	other (other)	Azodyrecin A, Azodyrecin B, Azodyrecin C	Streptomyces mirabilis strain P8-A
BGC0000188.5	0.36	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
diterpene			C20	C6-C11 + C10-C15	PP

Genes with terpene domains:

FED44_RS22160: T2TS

Domain 1 (10 - 507): Type 2 terpene synthase

Subtype: Diterpene synthase

Reactions:: GGPP → diterpene_C6-C11/C10-C15_PP

FED44_RS22165: T1TS

Domain 1 (12 - 280): Type 1 terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.30 out of 27.78

Confidence: medium

Additional weak hits (5):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.79 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.75 out of 30.25

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.06 out of 21.55

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.87 out of 36.53

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.67 out of 26.06

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria

NZ_VANP01000009.1 - Region 1 - blactam

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Location: 71,483 - 93,312 nt. (total: 21,830 nt) Show pHMM detection rules used

blactam: (BLS or CAS or tabtoxin)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase

FED44_RS22950: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: dehalogenase hydrolase

FED44_RS22980: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

FED44_RS22985: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000846.5	0.50	other (other)	tabtoxin	Pseudomonas syringae
BGC0000885.3	0.45	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0001880.3	0.41	ribosomal	scytodecamide	Scytonema sp. UIC 10036
BGC0001545.3	0.38	NRPS	chrysogine	Fusarium graminearum PH-1
BGC0001374.5	0.37	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001575.4	0.36	NRPS	PZN5, PZN6, PZN7, PZN9, PZN10, PZN11	Ruminococcus sp. 5_1_39BFAA
BGC0000937.5	0.34	other (other)	fosfazinomycin A, fosfazinomycin B	Streptomyces sp. WM6372
BGC0002499.2	0.33	NRPS, PKS	enteromycin	Streptomyces achromogenes subsp. streptozoticus
BGC0001739.3	0.33	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525
BGC0001007.5	0.33	NRPS, PKS	lymphostin, neolymphostinol B, lymphostinol, neolymphostin b	Salinispora arenicola CNS-205

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000846.5	0.50	other (other)	tabtoxin	Pseudomonas syringae
BGC0000885.3	0.45	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0001880.3	0.41	ribosomal	scytodecamide	Scytonema sp. UIC 10036
BGC0001545.3	0.38	NRPS	chrysogine	Fusarium graminearum PH-1
BGC0001374.5	0.37	other (other)	thienodolin	Streptomyces albogriseolus
BGC0001575.4	0.36	NRPS	PZN5, PZN6, PZN7, PZN9, PZN10, PZN11	Ruminococcus sp. 5_1_39BFAA
BGC0000937.5	0.34	other (other)	fosfazinomycin A, fosfazinomycin B	Streptomyces sp. WM6372
BGC0002499.2	0.33	NRPS, PKS	enteromycin	Streptomyces achromogenes subsp. streptozoticus
BGC0001739.3	0.33	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525
BGC0001007.5	0.33	NRPS, PKS	lymphostin, neolymphostinol B, lymphostinol, neolymphostin b	Salinispora arenicola CNS-205

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (10):

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.88 out of 23.43

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.49 out of 32.74

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.96 out of 26.06

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.53 out of 32.74

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.50 out of 30.98

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.43 out of 33.14

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.22 out of 23.43

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.37 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.19 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.95 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_VANP01000009.1 - Region 2 - terpene

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Location: 141,771 - 166,699 nt. (total: 24,929 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: sulfotransferase

FED44_RS23315: Sulfotransferase domain

extras: Sulfotransfer_3: Sulfotransferase domain

Lyases: cyclase

FED44_RS23335: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: synthetase

FED44_RS23345: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.80	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002708.2	0.75	terpene	(+)-δ-cadinol	Coniophora puteana RWD-64-598 SS2
BGC0000657.5	0.57	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0002309.2	0.47	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000677.5	0.47	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001911.3	0.42	terpene	raimonol	Streptomyces anulatus
BGC0000927.3	0.41	other (flavin)	roseoflavin	Streptomyces davaonensis JCM 4913
BGC0001910.4	0.41	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0002316.2	0.40	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.80	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002708.2	0.75	terpene	(+)-δ-cadinol	Coniophora puteana RWD-64-598 SS2
BGC0000657.5	0.57	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0002309.2	0.47	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000677.5	0.47	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001911.3	0.42	terpene	raimonol	Streptomyces anulatus
BGC0000927.3	0.41	other (flavin)	roseoflavin	Streptomyces davaonensis JCM 4913
BGC0001910.4	0.41	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0002316.2	0.40	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

FED44_RS23335: T1TS + T1TS

Domain 1 (2 - 321): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (340 - 685): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

FED44_RS23345: PT_FPPS_like

Domain 1 (54 - 356): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (2):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.45 out of 25.75

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate

TFBS definitions

Regulator	Description

NZ_VANP01000009.1 - Region 3 - NAPAA

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Location: 179,415 - 213,473 nt. (total: 34,059 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

FED44_RS23560: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001249.5	0.60	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001249.5	0.60	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.62 out of 19.52

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.37 out of 23.79

Confidence: medium

Additional weak hits (13):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.09 out of 21.55

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.23 out of 20.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.25 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.58 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.65 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.68 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.20 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.63 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.86 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.99 out of 27.78

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.79 out of 30.97

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.11 out of 26.33

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.02 out of 27.04

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 179414 - 213473:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

FED44_RS23535: X

AMP-binding (28..423): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FVVMFDFSVEEPYCLVGGELNTYGPTEATVTATC
Nearest Stachelhaus code(s):: Lys DIESIGTVTK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
IolR	Regulator of the inositol catabolism