LOCUS       NZ_VANP01000037         2973 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Microbispora fusca strain NEAU-HEGS1-5 Scaffold37, whole genome
            shotgun sequence.
ACCESSION   NZ_VANP01000037
VERSION     NZ_VANP01000037.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbispora fusca
  ORGANISM  Microbispora fusca
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Microbispora.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Yu,B.
  TITLE     Isolation, diversity and antifungal activity of Actinobacteria from
            wheat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Yu,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Northeast Agricultural University, School of
            Life Science, Mucai59, Harbin, Heilongjiang 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005864065.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:18:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,124
            CDSs (total)                      :: 7,053
            Genes (coding)                    :: 6,990
            CDSs (with protein)               :: 6,990
            Genes (RNA)                       :: 71
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3, 4 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 11 of 63
            Pseudo Genes (incomplete)         :: 57 of 63
            Pseudo Genes (internal stop)      :: 6 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:39:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2973
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VANP01000037.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS             complement(<1..2969)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (275-339). E-value: 3.8e-20. Score:
                     63.8. Matches aSDomain: nrpspksdomains_FED44_RS35370_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (362-644). E-value:
                     1.1e-80. Score: 262.8. Matches aSDomain:
                     nrpspksdomains_FED44_RS35370_Condensation_LCL.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="FED44_RS35370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_35370"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_138463397.1"
                     /sec_met_domain="PP-binding (E-value: 4.6e-18, bitscore:
                     51.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.7e-65, bitscore:
                     206.5, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.6e-44, bitscore:
                     137.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLYGPTEDTTYSTHAVTDPGAERTPLGRALPGSRVHLLDRELR
                     PVPLGAIGEVYLSGSGVTRGYHQRPALTAERYLPLPDDEGGRLYRTGDLARWRPCTTAD
                     GGVEFFLDYHGRVDHQVKLRGFRIEPGEVEHALLQHPAVSEAVVTVHGDHLIAHIASKN
                     TPTRDHGNHAQGDERAHDGHSDHTGTHDMHGGRPAVAADHGDLALCDADCEQILLRYLR
                     GCLPQHLVPARVVVMEALPRTPNGKIDRKALPEPSLTTAGAGEVEPPGTEAQRLVAGLF
                     AGLLGRPVTNIHEDFFSLGGHSLLATRLVHRLSEALGVVVPLRLVFDHPTVAGLAERLP
                     GVGAGEVVAIPVGRHPAGDHVHPATANQQRLWFLCHLHPDTNLAYHITGAATVRGPLDR
                     DVLRTALADTARRHESLRTTLREENEELVQVVSAEPVADLEEVTASDWAAVLDEEASRP
                     LDLAAGPLMRTVLVRVSDEEHVLLLSLHHLICDGWSLDLLLREITGHYARLAEGPAGNE
                     APSPASVQFADVELWERPVTDEDMDFWREHLAGAQTLDLPTDRPRPPYQTHRGDAVPLS
                     LPAATVAAVARATGASTFSVVATALGVLLAKLSGQDDVTIGFAASGRTRPELTDVIGML
                     VTTLPLRMRPAPETTLRDLLRATHDAALATQQHQHVGFDRLINECVRERDLSRSPLFQV
                     MLSINGTPPQYEFPGLAVDPVPVHPRASQFELSVHLEQSVEGLGGFLTYNTDLFDARSA
                     CLLAERLTVVVEALAADPGARVVDLDVRTAGEKTVLGALGDGGLLTGPSVGAGGCVHGL
                     VEAVVDRCPDAVAVRAVDGVVRYRELDERAEWLARHLVGLGARAGVLVGVCLPRTSDLI
                     VALLAVLKTGAAYVPIDPGYPPERVAYLLADSEAAVLVTTPDHNPLAGSAGSAGVQVVD
                     VRAVLGPVGGSPGSGRPGRLGVSVGAGDTAYVIYTSGSTGRPKGVVIEHRQTVAMLTWA
                     G"
     source          1..2973
                     /collection_date="2016-05-10"
                     /country="China: Zhumadian"
                     /db_xref="taxon:2576905"
                     /host="wheat"
                     /isolation_source="ear"
                     /mol_type="genomic DNA"
                     /organism="Microbispora fusca"
                     /strain="NEAU-HEGS1-5"
                     /submitter_seqid="Scaffold37"
                     /type_material="type strain of Microbispora fusca"
     gene            complement(<1..>2973)
                     /locus_tag="FED44_RS35370"
                     /old_locus_tag="FED44_35370"
     region          1..2973
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2973
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2973
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2969](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2969)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(31..90)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35370_0008"
                     /evalue="1.80E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="FED44_RS35370"
                     /protein_end="979"
                     /protein_start="959"
                     /score="35.6"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVVIEH"
     misc_feature    complement(81..83)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       complement(304..345)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35370_0007"
                     /evalue="7.30E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="FED44_RS35370"
                     /protein_end="888"
                     /protein_start="874"
                     /score="19.0"
                     /tool="antismash"
                     /translation="LAVLKTGAAYVPID"
     misc_feature    complement(321..323)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       complement(877..993)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35370_0006"
                     /evalue="9.10E-12"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="FED44_RS35370"
                     /protein_end="697"
                     /protein_start="658"
                     /score="37.3"
                     /tool="antismash"
                     /translation="HDAALATQQHQHVGFDRLINECVRERDLSRSPLFQVMLS"
     aSDomain        complement(1036..1881)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS35370_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.10E-80"
                     /label="FED44_RS35370_Condensation_LCL.1"
                     /locus_tag="FED44_RS35370"
                     /protein_end="644"
                     /protein_start="362"
                     /score="262.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHLICDG"
                     /tool="antismash"
                     /translation="PATANQQRLWFLCHLHPDTNLAYHITGAATVRGPLDRDVLRTALA
                     DTARRHESLRTTLREENEELVQVVSAEPVADLEEVTASDWAAVLDEEASRPLDLAAGPL
                     MRTVLVRVSDEEHVLLLSLHHLICDGWSLDLLLREITGHYARLAEGPAGNEAPSPASVQ
                     FADVELWERPVTDEDMDFWREHLAGAQTLDLPTDRPRPPYQTHRGDAVPLSLPAATVAA
                     VARATGASTFSVVATALGVLLAKLSGQDDVTIGFAASGRTRPELTDVIGMLVTTLPLRM
                     R"
     CDS_motif       complement(1042..1131)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35370_0005"
                     /evalue="5.00E-11"
                     /label="C5_LCL_267-296"
                     /locus_tag="FED44_RS35370"
                     /protein_end="642"
                     /protein_start="612"
                     /score="35.0"
                     /tool="antismash"
                     /translation="DVTIGFAASGRTRPELTDVIGMLVTTLPLR"
     CDS_motif       complement(1450..1515)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35370_0004"
                     /evalue="2.80E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="FED44_RS35370"
                     /protein_end="506"
                     /protein_start="484"
                     /score="29.5"
                     /tool="antismash"
                     /translation="LHHLICDGWSLDLLLREITGHY"
     CDS_motif       complement(1708..1818)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35370_0003"
                     /evalue="1.60E-13"
                     /label="C2_LCL_024-062"
                     /locus_tag="FED44_RS35370"
                     /protein_end="420"
                     /protein_start="383"
                     /score="43.0"
                     /tool="antismash"
                     /translation="AYHITGAATVRGPLDRDVLRTALADTARRHESLRTTL"
     aSDomain        complement(1951..2142)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS35370_PCP.1"
                     /evalue="3.80E-20"
                     /label="FED44_RS35370_PCP.1"
                     /locus_tag="FED44_RS35370"
                     /protein_end="339"
                     /protein_start="275"
                     /score="63.8"
                     /tool="antismash"
                     /translation="LVAGLFAGLLGRPVTNIHEDFFSLGGHSLLATRLVHRLSEALGVV
                     VPLRLVFDHPTVAGLAERL"
     CDS_motif       complement(2566..2631)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35370_0002"
                     /evalue="1.40E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="FED44_RS35370"
                     /protein_end="134"
                     /protein_start="112"
                     /score="36.3"
                     /tool="antismash"
                     /translation="YHGRVDHQVKLRGFRIEPGEVE"
     CDS_motif       complement(2740..2826)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35370_0001"
                     /evalue="2.00E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="FED44_RS35370"
                     /protein_end="76"
                     /protein_start="47"
                     /score="45.7"
                     /tool="antismash"
                     /translation="PLGAIGEVYLSGSGVTRGYHQRPALTAER"
ORIGIN
        1 gccccgccca ggtcagcatc gccaccgtct gccgatgctc gatcaccaca cccttcggcc
       61 gccccgtcga ccccgacgta taaatcacat acgccgtatc ccccgcgccg acggagacac
      121 ccaaccgacc aggacggccg gaaccggggc ttcccccgac cgggccgagc accgcgcgaa
      181 catcgacgac ctgcacccca gccgagcccg cggaaccggc cagggggttg tggtcgggtg
      241 tggtcaccag cacggccgcc tcgctgtcgg ccagcaggta ggccacccgc tccggcggat
      301 atcccggatc gatcggcaca taagccgcgc cggtcttcaa caccgccagc aacgccacga
      361 tcagatcact ggtccgcggc aggcacaccc ccaccaacac cccagcccgc gcacccaacc
      421 ccaccagatg acgagccaac cactcagccc gctcatccaa ctcccgatac cgcaccaccc
      481 cgtcgaccgc ccgcaccgcc accgcatccg gacaccgatc caccaccgcc tcgaccaacc
      541 catgcacaca cccaccagcc ccgacggatg gaccggtcag caagccaccg tcacccaacg
      601 caccaaggac ggtcttctcc cccgccgtac ggacatcgag atccaccacc cgcgcgccgg
      661 gatcggcggc cagcgcctcc accaccaccg tcaaccgctc cgccagcaga cacgccgacc
      721 gcgcatcgaa cagatccgtg ttgtaggtca ggaacccgcc caacccctcc accgactgct
      781 ccagatgcac cgacaactcg aactggctgg ctcggggatg caccggcacc gggtcgaccg
      841 cgagcccggg gaactcgtac tgcggtggcg tgccgttgat cgacagcatg acctggaaga
      901 gaggactgcg gctgaggtcg cgttcccgta cgcactcgtt gatcaggcga tcgaagccga
      961 cgtgctggtg ctgctgggtg gcgagcgcgg cgtcgtgcgt ggcgcgcagc aggtcacgga
     1021 gagtcgtctc cggcgccggg cgcatgcgca ggggcagcgt cgtgacgagc atgccgatga
     1081 catccgtgag ttcgggccgg gtacgccctg aggcggcgaa cccgatggtg acgtcgtcct
     1141 ggccgctgag cttggccagg agcacaccca gcgccgtcgc gacgacggag aacgtgcttg
     1201 ccccggtcgc ccgggccacg gcggccacgg tcgcggcggg cagggacagc ggaacggcgt
     1261 ccccacggtg cgtctggtac ggcgggcgtg gcctgtccgt gggcaggtcg agcgtctgcg
     1321 cgccggcgag gtgctcgcgc cagaaatcca tgtcctcgtc ggtgacaggc cgctcccaca
     1381 gctcgacgtc cgcgaactgc acagacgcgg gtgagggggc ctcgtttccc gccggacctt
     1441 cggccagcct ggcgtagtgg ccggtgatct cacggagcag caggtcgagc gaccagccgt
     1501 cgcagatgag gtgatgcagg ctcaggagca gcacgtgctc ctcgtccgac accctgacca
     1561 ggaccgtacg catcaacggc ccggcggcga ggtcgagcgg acgggacgcc tcctcgtcga
     1621 gcaccgcggc ccagtcggac gcggtcactt cctccaggtc ggcgaccggt tccgcggaca
     1681 ccacctgtac gagctcctcg ttctcctcac ggagggtggt gcgcaggctt tcgtggcgcc
     1741 gggcggtgtc ggcgagggcg gtgcgcagca catcccggtc gagcggcccg cgcacggtcg
     1801 ccgcgccggt gatgtggtac gccagattcg tgtccgggtg cagatgacac aagaaccaca
     1861 accgctgctg attcgccgtc gccggatgca catgatcacc agccggatga cgacccaccg
     1921 gaatcgccac cacctcaccg gcacccacac ccggcaaccg ctcagccaga ccggccaccg
     1981 tcggatgatc gaacaccagc cgaagcggca ccaccacacc cagcgcctcc gacaaccgat
     2041 gcaccaaccg cgtggccagc aacgaatgcc cacccagact gaagaagtcc tcatggatgt
     2101 tcgtgaccgg ccggcccagc agaccggcga acaacccggc caccagacgc tgcgcctcag
     2161 tgccgggagg ctccacttca ccggccccgg cggtcgtgag cgacggctcg ggcagcgcct
     2221 tacggtcgat cttcccgttc ggcgtacgcg gcagcgcctc catcaccaca acacgggcag
     2281 gcaccaaatg ctgcggcaga cacccccgca gataccgcag aagaatctgc tcgcaatccg
     2341 catcgcacag ggcaaggtca ccgtgatccg ccgcaacggc aggacgacca ccatgcatgt
     2401 catgcgtgcc ggtgtggtca ctgtgcccgt cgtgggctcg ctcgtcgccc tgcgcatggt
     2461 tgccgtggtc gcgggtggga gtgttcttgc tggcgatgtg ggcgatcagg tggtcgccat
     2521 ggacggtgac gacggcctcc gacacggcgg gatgctggag cagggcgtgt tccacctcgc
     2581 ccggctcgat ccggaacccc cggagcttca cctggtgatc cacccggccg tggtagtcca
     2641 ggaagaactc caccccaccg tccgcggtgg tgcaggggcg ccagcgggcc agatcccctg
     2701 tccgatacag ccgcccaccc tcatcgtcgg ggaggggcag gtagcgttcg gccgtcaggg
     2761 cgggccgctg gtggtacccc cgcgtcaccc cgctcccgga caggtacacc tcgccgatcg
     2821 cgcccagcgg caccggacgc agctccctgt ccagcaggtg cacgcgtgat ccgggcaggg
     2881 cgcggcccag cggggtccgc tcagcaccgg gatcggtcac cgcgtgggtg gagtacgtgg
     2941 tgtcctcggt cggcccgtac aggttgttga cca
//
