LOCUS       NZ_VANP01000032         6013 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Microbispora fusca strain NEAU-HEGS1-5 Scaffold32, whole genome
            shotgun sequence.
ACCESSION   NZ_VANP01000032
VERSION     NZ_VANP01000032.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbispora fusca
  ORGANISM  Microbispora fusca
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Microbispora.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Yu,B.
  TITLE     Isolation, diversity and antifungal activity of Actinobacteria from
            wheat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Yu,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Northeast Agricultural University, School of
            Life Science, Mucai59, Harbin, Heilongjiang 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005864065.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:18:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,124
            CDSs (total)                      :: 7,053
            Genes (coding)                    :: 6,990
            CDSs (with protein)               :: 6,990
            Genes (RNA)                       :: 71
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3, 4 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 11 of 63
            Pseudo Genes (incomplete)         :: 57 of 63
            Pseudo Genes (internal stop)      :: 6 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:39:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 6013
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VANP01000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..6013
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:>6009](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..>6009)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     gene            complement(<1..2087)
                     /locus_tag="FED44_RS35280"
                     /old_locus_tag="FED44_35280"
     CDS             complement(<1..2087)
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (3-30). E-value:
                     9.5e-10. Score: 29.9. Matches aSDomain:
                     nrpspksdomains_FED44_RS35280_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (35-461). E-value:
                     1.5e-185. Score: 609.1. Matches aSDomain:
                     nrpspksdomains_FED44_RS35280_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082190.1"
                     /locus_tag="FED44_RS35280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_35280"
                     /product="type I polyketide synthase"
                     /protein_id="WP_138463387.1"
                     /sec_met_domain="mod_KS (E-value: 4.5e-234, bitscore:
                     764.0, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 6.7e-97, bitscore:
                     311.0, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 6.6e-26, bitscore: 78.4,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MANDDKLREYLKRSIAETKQAEKRLRQIEAKSREPIAIVGMGCRL
                     PGGVASPEDLWDLVAEGRDAVTEFPADRGWDLDALYDPDPDQPGTSYTRHGGFLDDVAG
                     FDAGFFGISRREALASDPQQRLLLEVAWETLERAGIDPATLKGSRTAVYAGVADRTYGS
                     RLPEVPEDLEGYLAIGNLGSVLSGRIAYTFGFEGPAVTVDTACSSSLVALHLAVQALRT
                     GECDLALAGGVTVMASPIGFTEFSRQRGLAPDGRCKAFSSSADGTGWAEGVGLLLVERL
                     SDARRNGHPVLAVVRGTAINQDGASNGLTAPNGPSQQRVITHALHNAGLTPADVDAVEA
                     HGTGTTLGDPIEAQALLATYGHNRPADRPLWLGSLKSNIGHAQAAAGVAGIIKTVHAIR
                     NGLLPQTLHVDQPTPHVDWTTGSIKLLTESQPWPDHGHPRRAAVSAFGVSGTNAHVIIE
                     QAPDQTDAATTVETPPNGSRATSAGSLAPAEAGHQAPATQDPGAHDTPRPVEVSQGRGV
                     PVVGWVVSARSPEALRAQAARLADHLDRHPSLDAVDIAHSLATTRAHFDHRAVITGTTP
                     QELLERTRALADGRITPGIVTGVARPGKVAFVFTGQGSQRPGMGRGLYAAYPTFATAFD
                     HVIQALDEHLAGHTPVPLREVLLGDADQRLLDQTLYTQPALFALQTALTHLLATWGITP
                     HT"
     source          1..6013
                     /collection_date="2016-05-10"
                     /country="China: Zhumadian"
                     /db_xref="taxon:2576905"
                     /host="wheat"
                     /isolation_source="ear"
                     /mol_type="genomic DNA"
                     /organism="Microbispora fusca"
                     /strain="NEAU-HEGS1-5"
                     /submitter_seqid="Scaffold32"
                     /type_material="type strain of Microbispora fusca"
     region          1..6013
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..6013
                     /SMILES="C(C)C(=O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     CDS_motif       complement(216..269)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35280_0005"
                     /evalue="1.00E-06"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="FED44_RS35280"
                     /protein_end="624"
                     /protein_start="606"
                     /score="20.9"
                     /tool="antismash"
                     /translation="FVFTGQGSQRPGMGRGLY"
     aSDomain        complement(705..1982)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS35280_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.50E-185"
                     /label="FED44_RS35280_PKS_KS.1"
                     /locus_tag="FED44_RS35280"
                     /protein_end="461"
                     /protein_start="35"
                     /score="609.1"
                     /tool="antismash"
                     /translation="IAIVGMGCRLPGGVASPEDLWDLVAEGRDAVTEFPADRGWDLDAL
                     YDPDPDQPGTSYTRHGGFLDDVAGFDAGFFGISRREALASDPQQRLLLEVAWETLERAG
                     IDPATLKGSRTAVYAGVADRTYGSRLPEVPEDLEGYLAIGNLGSVLSGRIAYTFGFEGP
                     AVTVDTACSSSLVALHLAVQALRTGECDLALAGGVTVMASPIGFTEFSRQRGLAPDGRC
                     KAFSSSADGTGWAEGVGLLLVERLSDARRNGHPVLAVVRGTAINQDGASNGLTAPNGPS
                     QQRVITHALHNAGLTPADVDAVEAHGTGTTLGDPIEAQALLATYGHNRPADRPLWLGSL
                     KSNIGHAQAAAGVAGIIKTVHAIRNGLLPQTLHVDQPTPHVDWTTGSIKLLTESQPWPD
                     HGHPRRAAVSAFGVSGTNAHVIIEQAP"
     CDS_motif       complement(927..971)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35280_0004"
                     /evalue="1.10E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="FED44_RS35280"
                     /protein_end="387"
                     /protein_start="372"
                     /score="11.5"
                     /tool="antismash"
                     /translation="GSLKSNIGHAQAAAG"
     CDS_motif       complement(1032..1076)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35280_0003"
                     /evalue="1.40E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="FED44_RS35280"
                     /protein_end="352"
                     /protein_start="337"
                     /score="13.8"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       complement(1452..1499)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35280_0002"
                     /evalue="2.90E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="FED44_RS35280"
                     /protein_end="212"
                     /protein_start="196"
                     /score="22.2"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       complement(1701..1727)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35280_0001"
                     /evalue="9.00E-03"
                     /label="PKSI-KS_m2"
                     /locus_tag="FED44_RS35280"
                     /protein_end="129"
                     /protein_start="120"
                     /score="9.0"
                     /tool="antismash"
                     /translation="DPQQRLLLE"
     aSDomain        complement(1998..2078)
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS35280_PKS_Docking_Nterm.
                     1"
                     /evalue="9.50E-10"
                     /label="FED44_RS35280_PKS_Docking_Nterm.1"
                     /locus_tag="FED44_RS35280"
                     /protein_end="30"
                     /protein_start="3"
                     /score="29.9"
                     /tool="antismash"
                     /translation="DDKLREYLKRSIAETKQAEKRLRQIEA"
     misc_feature    complement(2087..2096)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.52"
                     /tool="antismash"
     CDS             complement(2094..>6009)
                     /NRPS_PKS="Domain: PKS_PP (114-198). E-value: 3e-28. Score:
                     90.0. Matches aSDomain:
                     nrpspksdomains_FED44_RS35285_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (225-651). E-value:
                     2.7e-185. Score: 608.2. Matches aSDomain:
                     nrpspksdomains_FED44_RS35285_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (764-1062). E-value: 4.3e-110.
                     Score: 359.7. Matches aSDomain:
                     nrpspksdomains_FED44_RS35285_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_PP (1142-1225). E-value: 1.4e-29.
                     Score: 94.3. Matches aSDomain:
                     nrpspksdomains_FED44_RS35285_PKS_PP.2"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (1242-1304). E-value:
                     1.2e-09. Score: 30.8. Matches aSDomain:
                     nrpspksdomains_FED44_RS35285_PKS_Docking_Cterm.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /locus_tag="FED44_RS35285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_35285"
                     /product="type I polyketide synthase"
                     /protein_id="WP_138463388.1"
                     /sec_met_domain="mod_KS (E-value: 1e-235, bitscore: 769.4,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 9.1e-98, bitscore:
                     313.9, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.4e-111, bitscore:
                     359.7, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1e-16, bitscore:
                     48.5, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TFHDALAHHRHTQNLPATSLAWGLWAHTSTLTAGLGQNDHKRLAR
                     LGLRPLETDHALALFDAVVFSAAAQPPVLVPARLNTATDAPPPLLRRLISPRRRAVRAA
                     AGQDGSLTTRLSALTPERRSAALLDLVRTEVATVLSHPDPHTIPPDRPLIELGLDSLTA
                     VELRNRLTTTTHLRLPATLTFDHPTPEGIAELLDGLLGGDVRAEKAPVRAETTATGSLA
                     DDPVVIVGMACRLPGGIESPEALWRLVERGGDAVSEFPADRGWDLERLYDPDPDHLGTS
                     YTRHGGFLRDVADFDATFFGISPREALATDPQQRLLLEVAWEAFERAGIDPRRLRGSRT
                     GVFAGVMYHDYAPRLQEIPGDLEGYLVNGSAGSIASGRVAYTFGFEGPAVTVDTACSSS
                     LVALHLAAQSLRTGESDLALAGGVAVMSSPATFVEFSRQRALAADGRCKAFSSSADGTG
                     WGEGVALLLLERLSDARRNDHPVLAVVRGSAVNQDGASNGLTAPSGPSQQRVIRQALAN
                     AGLTTGEVDVVEAHGTGTTLGDPIEAQALLATYGQDRPAERPLWLGSLKSNIAHTQAAA
                     GGAGVIKMIMAMRAGVLPKTLHVDEPTPHVDWSSGGVRLLDEARPWPEDGGPRRAGVSS
                     FGVSGTNAHVILEQLPAAEEPVRTPGTPPPGTRPAVPPAVPWVLSGHLPEALRGQAVSL
                     ATYLDGRADLDPVDVAHSLVSARTLLAERAVVVAPDAEGLTSGLAALTREGALPGHVVR
                     GTADVAGKVVFVFPGQGGQWPGMAAGLLESAPVFAKRLAECERALSEFVDWSLLDVVRG
                     VPGAPGLDRVDVVQPVLWAVMVSLAELWQSYGVRPDAVVGHSQGEIAAAVVAGGLSLRD
                     AARVVALRSQAIRTLSGDGGMASVALPLEEAEARIAPWDGRLSVGVVNGPGSVVVSGEV
                     GALAELLAALDAEGVRNRRLPVDYASHSAQVELISDELPKALSPITPRALTVPMLSTVT
                     GEWAGEAELDADYWYANLRRPVRFEAATRALAASGHTAFLEISPHPVLASSIEDTLEAA
                     AVAPALVTGTLRRDEGGPERFLVSLAEAFVRGVPVDWAVALRGADPRRVDLPTYAFQRT
                     RFWLDASRTSSATSSEGGVNDGAAGGQAPEPASRFAGLDPEQRGEAVLDLVRTEVAAVL
                     RHADGAEIPPARAFRELGLDSLTAVDLRNRLRTATGLPLPTTLIFDHPSPAAVTDYILS
                     ALAEAEVDGDGEPAGDPLTALRRLEASLLAEPGGRGEVVSRLRSLLARFDTPLGADDDP
                     DDEIDLDSATDDELFDLLDRS"
     gene            complement(2094..>6013)
                     /locus_tag="FED44_RS35285"
                     /old_locus_tag="FED44_35285"
     aSDomain        complement(2097..2282)
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS35285_PKS_Docking_Cterm.
                     1"
                     /evalue="1.20E-09"
                     /label="FED44_RS35285_PKS_Docking_Cterm.1"
                     /locus_tag="FED44_RS35285"
                     /protein_end="1304"
                     /protein_start="1242"
                     /score="30.8"
                     /tool="antismash"
                     /translation="LTALRRLEASLLAEPGGRGEVVSRLRSLLARFDTPLGADDDPDDE
                     IDLDSATDDELFDLLDR"
     CDS_motif       complement(2280..2318)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35285_0009"
                     /evalue="9.90E+01"
                     /label="PKSI-KS_m5"
                     /locus_tag="FED44_RS35285"
                     /protein_end="1243"
                     /protein_start="1230"
                     /score="-1.9"
                     /tool="antismash"
                     /translation="EVDGDGEPAGDPL"
     aSDomain        complement(2334..2582)
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS35285_PKS_PP.2"
                     /evalue="1.40E-29"
                     /label="FED44_RS35285_PKS_PP.2"
                     /locus_tag="FED44_RS35285"
                     /protein_end="1225"
                     /protein_start="1142"
                     /score="94.3"
                     /tool="antismash"
                     /translation="AGLDPEQRGEAVLDLVRTEVAAVLRHADGAEIPPARAFRELGLDS
                     LTAVDLRNRLRTATGLPLPTTLIFDHPSPAAVTDYILS"
     aSModule        complement(2334..5333)
                     /complete
                     /domains="nrpspksdomains_FED44_RS35285_PKS_KS.1"
                     /domains="nrpspksdomains_FED44_RS35285_PKS_AT.1"
                     /domains="nrpspksdomains_FED44_RS35285_PKS_PP.2"
                     /locus_tags="FED44_RS35285"
                     /monomer_pairings="mmal -> Me-mal"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(2823..3716)
                     /ASF="Methylmalonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS35285_PKS_AT.1"
                     /evalue="4.30E-110"
                     /label="FED44_RS35285_PKS_AT.1"
                     /locus_tag="FED44_RS35285"
                     /protein_end="1062"
                     /protein_start="764"
                     /score="359.7"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFPGQGGQWPGMAAGLLESAPVFAKRLAECERALSEFVDWSLLDV
                     VRGVPGAPGLDRVDVVQPVLWAVMVSLAELWQSYGVRPDAVVGHSQGEIAAAVVAGGLS
                     LRDAARVVALRSQAIRTLSGDGGMASVALPLEEAEARIAPWDGRLSVGVVNGPGSVVVS
                     GEVGALAELLAALDAEGVRNRRLPVDYASHSAQVELISDELPKALSPITPRALTVPMLS
                     TVTGEWAGEAELDADYWYANLRRPVRFEAATRALAASGHTAFLEISPHPVLASSIEDTL
                     EAAAVAPALVTGTLRRD"
     CDS_motif       complement(2898..2927)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35285_0008"
                     /evalue="6.70E-03"
                     /label="PKSI-AT-mM_m9"
                     /locus_tag="FED44_RS35285"
                     /protein_end="1037"
                     /protein_start="1027"
                     /score="9.3"
                     /tool="antismash"
                     /translation="FLEISPHPVL"
     CDS_motif       complement(2970..3017)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35285_0007"
                     /evalue="7.40E-06"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="FED44_RS35285"
                     /protein_end="1013"
                     /protein_start="997"
                     /score="17.9"
                     /tool="antismash"
                     /translation="LDADYWYANLRRPVRF"
     CDS_motif       complement(3123..3155)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35285_0006"
                     /evalue="3.20E-04"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="FED44_RS35285"
                     /protein_end="962"
                     /protein_start="951"
                     /score="13.4"
                     /tool="antismash"
                     /translation="VDYASHSAQVE"
     CDS_motif       complement(3369..3479)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35285_0005"
                     /evalue="1.30E-18"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="FED44_RS35285"
                     /protein_end="880"
                     /protein_start="843"
                     /score="58.7"
                     /tool="antismash"
                     /translation="GVRPDAVVGHSQGEIAAAVVAGGLSLRDAARVVALRS"
     CDS_motif       complement(3483..3554)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35285_0004"
                     /evalue="2.30E-10"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="FED44_RS35285"
                     /protein_end="842"
                     /protein_start="818"
                     /score="32.5"
                     /tool="antismash"
                     /translation="LDRVDVVQPVLWAVMVSLAELWQS"
     CDS_motif       complement(3666..3722)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35285_0003"
                     /evalue="3.10E-08"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="FED44_RS35285"
                     /protein_end="781"
                     /protein_start="762"
                     /score="25.5"
                     /tool="antismash"
                     /translation="VFVFPGQGGQWPGMAAGLL"
     aSDomain        complement(4056..5333)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS35285_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="2.70E-185"
                     /label="FED44_RS35285_PKS_KS.1"
                     /locus_tag="FED44_RS35285"
                     /protein_end="651"
                     /protein_start="225"
                     /score="608.2"
                     /tool="antismash"
                     /translation="VVIVGMACRLPGGIESPEALWRLVERGGDAVSEFPADRGWDLERL
                     YDPDPDHLGTSYTRHGGFLRDVADFDATFFGISPREALATDPQQRLLLEVAWEAFERAG
                     IDPRRLRGSRTGVFAGVMYHDYAPRLQEIPGDLEGYLVNGSAGSIASGRVAYTFGFEGP
                     AVTVDTACSSSLVALHLAAQSLRTGESDLALAGGVAVMSSPATFVEFSRQRALAADGRC
                     KAFSSSADGTGWGEGVALLLLERLSDARRNDHPVLAVVRGSAVNQDGASNGLTAPSGPS
                     QQRVIRQALANAGLTTGEVDVVEAHGTGTTLGDPIEAQALLATYGQDRPAERPLWLGSL
                     KSNIAHTQAAAGGAGVIKMIMAMRAGVLPKTLHVDEPTPHVDWSSGGVRLLDEARPWPE
                     DGGPRRAGVSSFGVSGTNAHVILEQLP"
     CDS_motif       complement(4383..4427)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35285_0002"
                     /evalue="2.50E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="FED44_RS35285"
                     /protein_end="542"
                     /protein_start="527"
                     /score="13.1"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       complement(4803..4850)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS35285_0001"
                     /evalue="6.10E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="FED44_RS35285"
                     /protein_end="402"
                     /protein_start="386"
                     /score="21.2"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     aSDomain        complement(5415..5666)
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS35285_PKS_PP.1"
                     /evalue="3.00E-28"
                     /label="FED44_RS35285_PKS_PP.1"
                     /locus_tag="FED44_RS35285"
                     /protein_end="198"
                     /protein_start="114"
                     /score="90.0"
                     /tool="antismash"
                     /translation="LSALTPERRSAALLDLVRTEVATVLSHPDPHTIPPDRPLIELGLD
                     SLTAVELRNRLTTTTHLRLPATLTFDHPTPEGIAELLDG"
ORIGIN
        1 gtgtgggggg tgatgcccca ggtggcgagc aggtgggtga gggcggtctg cagggcgaac
       61 agggcgggct gggtgtagag cgtctggtcc agcagcctct ggtcggcgtc cccgagcagg
      121 acctcgcgta gtggcaccgg ggtgtgtccg gccaggtgct catccagtgc ctggatgacg
      181 tggtcgaatg cggtggcgaa ggtggggtag gcggcgtaca ggccgcgtcc catgccgggg
      241 cgttggctgc cttggccggt gaacacgaac gcgaccttgc cgggacgggc gaccccggtg
      301 acgatgccgg gggtgatgcg gccgtcggcg agggcgcggg tgcgctccag gagttcctgt
      361 ggtgtggtgc cggtgatgac ggcgcggtgg tcgaagtggg cgcgggtggt ggccagggag
      421 tgcgcgatgt cgaccgcgtc caggctgggg tggcggtcga ggtggtcggc cagccgggcg
      481 gcctgggcgc gtagtgcttc gggtgaccgt gccgagacca cccaccccac caccggcacc
      541 ccacgcccct gggaaacctc caccggacgc ggagtgtcgt gggcaccggg gtcctgcgtg
      601 gccggtgcct gatgcccggc ctccgccggc gccaggctgc cggcggacgt cgcgcgtgac
      661 ccgttcggtg gcgtttccac ggtcgtggcc gcgtcagtct ggtcgggtgc ctgttcgatg
      721 atgacgtggg cgttggtgcc gctgacgccg aaggcggaga cggcggcgcg gcgggggtgg
      781 ccgtggtcgg gccagggctg ggattcggtg aggagtttga tgctgccggt ggtccagtcg
      841 acgtgggggg tgggctggtc gacgtggagg gtttggggga gcaggccgtt gcggatggcg
      901 tggacggttt tgatgattcc ggcgacgccg gcggcggctt gggcgtggcc gatgttggat
      961 ttgagggagc cgagccatag cgggcggtcg gcggggcggt tgtggccgta ggtggcgagc
     1021 aatgcctggg cttcgatggg gtcgccgagg gtggtgccgg tgccgtgggc ttcgacggcg
     1081 tcgacgtctg cgggggtgag tccggcgttg tggagggcgt gggtgatgac gcgttgctgg
     1141 gaggggccgt tgggtgcggt caggccgttg gaggcgccgt cttggttgat ggcggtgccg
     1201 cggacgacgg ccagcacggg gtgtccgttc ctccgcgcgt ccgacaggcg ttccaccagg
     1261 aggaggccga ctccttcggc ccagccggtg ccgtcggcgg aggaggagaa cgccttgcac
     1321 cgcccgtccg gcgcgagccc ccgctgccgg gagaactcgg tgaacccgat cgggctcgcc
     1381 atcaccgtca ccccgcccgc gagcgcgagg tcgcattccc ccgtacgcag ggcctggacg
     1441 gcgaggtgca gcgcgaccag tgacgacgag caggccgtgt ccacggtcac cgccgggccc
     1501 tcgaagccga aggtgtaggc gatccgcccc gacagcacgc tgcccaggtt gccgattgcc
     1561 agatagccct ccaggtcttc gggcacctcc ggcagccgtg agccgtacgt acggtcggcc
     1621 acccccgcgt acaccgccgt acggctgccc ttgagggtgg cagggtcgat gccggcgcgt
     1681 tcgagggttt cccaggcgac ttcgaggagg aggcgttgct gggggtcgct ggcgagtgct
     1741 tcgcggcggg agatgccgaa gaagccggcg tcgaatccgg cgacgtcgtc gaggaagccg
     1801 ccgtggcggg tgtaggaggt gccgggctgg tcggggtcgg ggtcgtagag ggcgtcgagg
     1861 tcccagccac ggtcggcggg gaactcggtg acggcgtcgc ggccctcggc gaccaggtcc
     1921 cacaggtcct cgggggaggc caccccgccg ggcaggcggc accccattcc cacgatcgcg
     1981 atcggctccc gcgacttcgc ctcgatctgc cgcagccgtt tctccgcctg cttggtctcc
     2041 gcgatcgagc gcttgagata ttcgcgcagc ttgtcgtcgt tcgccatcac aaatcagctc
     2101 ctgtccagga ggtcgaagag ttcgtcgtcg gtggcggagt cgaggtcgat ctcgtcgtcc
     2161 gggtcgtcgt ccgcgccgag cggggtgtcg aagcgggcca gcagtgagcg caggcgggag
     2221 acgacctcgc cccggccgcc gggctcggcg agcaggctcg cctccagccg ccgcagcgcg
     2281 gtcagcgggt cgccggcggg ttctccgtcg ccgtccacct cggcctcggc cagcgccgac
     2341 aggatgtagt cggtcaccgc ggcgggcgag ggatgatcga agatcagcgt ggtcggcagg
     2401 ggcagcccgg tcgccgtacg gagccggttg cgcaggtcca cggcggtcag cgagtcgagc
     2461 cccagctccc ggaacgccct ggccggcggg atctccgcac cgtcggcgtg ccgcagcacc
     2521 gcggcgacct ccgtgcggac gaggtcgagc acggcctcgc cgcgctgctc cgggtcgagc
     2581 ccggcgaacc gcgacgcggg ttcgggcgcc tgccccccgg cagcgccgtc gttcaccccg
     2641 ccctcggaag acgtggccga ggacgtacgc gaggcgtcga gccagaagcg ggtgcgctgg
     2701 aaggcgtagg tcggcaggtc gacgcggcgt gggtcggctc cgcggagggc gacggcccag
     2761 tcgaccggca cgccccgcac gaaggcctcg gcgagcgaca cgaggaaccg ctccggcccg
     2821 ccttcgtcgc gccggagcgt cccggtgacc agcgcgggcg cgaccgccgc cgcctccagg
     2881 gtgtcctcga tgctcgacgc cagcaccgga tgcgggctga tctccaggaa cgcggtgtga
     2941 ccactggccg ccagggcgcg ggtggcggcc tcgaaccgca cgggccgccg caggttcgcg
     3001 taccagtagt cggcgtcgag ctccgcctcg ccggcccact cgcccgtgac ggtcgacagc
     3061 atgggcacgg tcagcgcgcg gggcgtgatc ggggacagcg ccttcggcaa ctcgtcggag
     3121 atcagctcga cctgggcgga gtgcgaggcg tagtcgacgg ggagccgccg gttgcgcacc
     3181 ccctccgcgt cgagcgcggc cagcagttcg gccagggcgc ctacctcgcc ggacacgacc
     3241 acgctgcccg gcccgttgac gaccccgacc gacagccgtc cgtcccaggg cgcgatccgg
     3301 gcctcggcct cctccagcgg cagggccacc gacgccatgc cgccgtcgcc cgacagggtc
     3361 cggatggcct gactgcgcag ggccaccacg cgggcggcgt cgcgcagcga cagtccgccc
     3421 gccaccacgg ccgcggcgat ctctccctgc gagtggccca ccaccgcgtc gggacggacc
     3481 ccgtacgact gccagagctc ggcgagcgac accatgacgg cccacagcac cggctgcacc
     3541 acgtcgaccc ggtcgagccc cggcgcgccg gggacgccgc gcaccacgtc gagcagcgac
     3601 cagtcgacga actcgctcaa cgcccgctcg cactccgcga gccgtttcgc gaacacgggc
     3661 gccgactcca gcagcccggc ggccatgccg ggccactgcc cgccctgccc cgggaagacg
     3721 aacacgacct tcccggcgac gtcggccgtg ccgcgtacga cgtggccggg cagcgcgccc
     3781 tcgcgggtga gggcggccag gccggaggtc agcccctccg cgtccggcgc gaccacgacg
     3841 gcgcgctcgg ccagcagcgt cctggcggag acgagggagt gcgccacgtc gaccgggtcg
     3901 aggtccgccc ggccgtccag gtacgtcgcg aggctgacgg cctgaccgcg cagcgcctcg
     3961 ggcaggtgcc ccgacagcac ccacggcacg gcgggcggca cggcgggccg cgtgccgggc
     4021 ggcggcgtgc ccggcgtcct gaccggctcc tcggcggcgg ggagctgctc caggatcacg
     4081 tgcgcgttcg tcccgctcac cccgaacgac gacaccccgg cccgtctcgg gccgccgtcc
     4141 tccggccagg gccgcgcctc gtcgaggagg cggacgccgc cgctcgacca gtccacgtgc
     4201 ggggtcggct cgtcgacgtg cagggtcttc ggcagcaccc cggcgcgcat cgccatgatc
     4261 atcttgatca cgccggcacc gcccgccgcg gcctgggtgt gcgcgatgtt cgacttgagg
     4321 gaaccgagcc acagcggccg ctcggccggg cggtcctggc cgtacgtcgc cagcagcgcc
     4381 tgcgcctcga tcgggtcgcc gagcgtcgtg ccggtgccgt gcgcctcgac cacgtccacc
     4441 tcgccggtag tcagcccggc gttggcgagc gcctgccgga tgacccgctg ctgcgacggg
     4501 ccgctcgggg cggtcaggcc gttggacgcg ccgtcctggt tgacggccga gccgcggacc
     4561 accgcgagca ccggatggtc gttcctgcgt gcgtccgaca gccgttcgag gaggagcagg
     4621 gcgacgcctt ccccccagcc ggtgccgtcg gccgaggacg agaacgcctt gcaacgcccg
     4681 tcggccgcca gggcccgctg ccgggagaac tcgacgaacg tggcgggcga cgacatcacg
     4741 gcgacgccgc cggcgagcgc gaggtccgac tcacccgtac gcagcgactg cgcggccagg
     4801 tgcagcgcga ccagcgacga cgagcaggcc gtgtcgacgg tcaccgccgg gccctcgaag
     4861 ccgaacgtgt acgcgacgcg gcccgaggcg atgctgcccg cgctgccgtt gacgagatag
     4921 ccctccaggt cgcccgggat ctcctgcagc cggggcgcgt agtcgtggta catcaccccg
     4981 gcgaagaccc cggtgcggct gcccctgagc cggcgggggt cgatcccggc ccgctcgaac
     5041 gcctcccacg ccacttcgag gagcagccgc tgctgcgggt ccgtcgccag cgcctcacgg
     5101 ggcgagatgc cgaagaaggt ggcgtcgaag tcggcgacgt cccgcaggaa cccgccgtgc
     5161 cgggtgtagg aggtgccgag atggtccgga tcggggtcgt agagccgttc gaggtcccag
     5221 ccccggtcgg cggggaactc cgacacggcg tccccgccgc gctccaccag ccgccacagc
     5281 gcctccggcg actcgatgcc gcccgggagc cggcaggcca tcccgacgat gacgaccgga
     5341 tcgtccgcga gcgaccctgt cgcggtcgtc tcggcccgca ccggcgcctt ctccgcccgg
     5401 acgtccccgc cgagcaggcc gtccagcagt tcggcgatgc cctcgggggt ggggtggtcg
     5461 aaggtgagag tggcggggag tcggaggtgg gttgtggtgg tgaggcggtt gcggagttcg
     5521 acggcggtga gggagtcgag gccgagttcg atgagggggc ggtcgggggg gatggtgtgg
     5581 gggtcggggt ggctgaggac ggtggcgacc tcggtgcgga ccaggtcgag cagggccgcg
     5641 ctccgccgtt cgggagtcag cgcggagagt cgcgtcgtga gggagccgtc ctgcccggcg
     5701 gccgcccgca ccgcccggcg gcgtggggag atcaggcggc gcagcagggg cggcggcgcg
     5761 tcggtcgccg tgttcaggcg agccggtacg agcaccggcg gctgcgcggc cgcgctgaag
     5821 accaccgcgt cgaacagggc cagggcgtga tccgtttcta gcgggcgcag gccgagcctg
     5881 gccaggcgct tgtggtcgtt ctgaccgagt ccggcggtga gggtgctggt gtgggcccat
     5941 agtccccagg cgaggctggt ggcggggagg ttctgggtgt ggcggtggtg ggcgagggcg
     6001 tcgtggaagg tgt
//
