LOCUS       NZ_VANP01000018        22849 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Microbispora fusca strain NEAU-HEGS1-5 Scaffold18, whole genome
            shotgun sequence.
ACCESSION   NZ_VANP01000018
VERSION     NZ_VANP01000018.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbispora fusca
  ORGANISM  Microbispora fusca
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Microbispora.
REFERENCE   1  (bases 1 to 135272)
  AUTHORS   Yu,B.
  TITLE     Isolation, diversity and antifungal activity of Actinobacteria from
            wheat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 135272)
  AUTHORS   Yu,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Northeast Agricultural University, School of
            Life Science, Mucai59, Harbin, Heilongjiang 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005864065.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:18:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,124
            CDSs (total)                      :: 7,053
            Genes (coding)                    :: 6,990
            CDSs (with protein)               :: 6,990
            Genes (RNA)                       :: 71
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3, 4 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 11 of 63
            Pseudo Genes (incomplete)         :: 57 of 63
            Pseudo Genes (internal stop)      :: 6 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:39:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 67441
            Orig. end                         :: 90290
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VANP01000018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22849
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="2"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22849
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22849
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12849](-)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12849)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    complement(638..651)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.3"
                     /tool="antismash"
     misc_feature    complement(732..747)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.68"
                     /tool="antismash"
     misc_feature    1070..1083
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 17.99"
                     /tool="antismash"
     misc_feature    complement(1900..1915)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.23"
                     /tool="antismash"
     misc_feature    complement(2065..2080)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.43"
                     /tool="antismash"
     gene            2069..4849
                     /locus_tag="FED44_RS31630"
                     /old_locus_tag="FED44_31630"
     CDS             2069..4849
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1149: LuxR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890458.1"
                     /locus_tag="FED44_RS31630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31630"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_117409961.1"
                     /transl_table=11
                     /translation="MNLIERDEALTSLQEMLAAALTGKGKVAMLSGTVATGKSALLDAF
                     AEQAVELGALPVAATASRMEQDLPLGVLGQLIEEAPLVEEERVRALALLEEGKQTAMSS
                     GLASETLDQVDAQIAHALCTVLLELSERYPLVIVVDDVHYADRPSLLCLAYLARRVRFA
                     RIMVIFSYSDYVRSTDTFFWTDLLRQPYCGVVQLNPLSRQGVHTLVRERAGDDVAVRFA
                     EDWYAFSAGNPLLAAALIEDYLGGPRERDGEPVTDEPVVGERYGQAVLTCLHRGEPELL
                     RIARCLAVLGDTDSLDRLLGLGHTQISRAMNALTAGGVLSVGRFRHESARTAVLAEVGL
                     DERRDLHRRAAELAHADGAATSVIADHLVQAGPIDAPWVVPVLEDAARDALREGRTERG
                     VDYLRLAWQACTDERRRLKIATMLVRAEWRINPEAPDRHLADLTEAMQRGHLRGSDGVV
                     LARALLWHGRYDDAQDVIEHLTTAGYESDPETVMELNIARPWLRATHPAFASKLGEPRA
                     DTGTALVSVAAGRRLETATALAQVLVNGPHEGALIAVERILRLARLDEMSMDTVECALL
                     ALTYGGQAEKAAPLCDLFSVEASSRRAPSRQARLSAIRAEMAVRQGDMVSAEQYARAAL
                     EIMPITSWGVTVGSPLGSLVIALTAMGRFDDVHDILDRPVLDAMFESRYGLHYLYARGR
                     YSLAVGQAALALRDFQLCGELTGRWGLDVPGLLPWRAGAAEACLQMGRSEQASRLIEDQ
                     LSRCNSRTPRELGMTIRLLAATSELRHRPMLLRKSADMLQNGGDRYEQARSLFDLAEAY
                     RGLGEHRRVGMIMIRARALMKECGAEIGGAQAAAETGKQASPALSGGSAALLSEAERRV
                     AVLAAAGHTNREIAGELYLTVSTVEQHLTRIYRKLDISSRADLPAVLEFGGAAVG"
     misc_feature    2105..2107
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5047..6636
                     /locus_tag="FED44_RS31635"
                     /old_locus_tag="FED44_31635"
     CDS             5047..6636
                     /EC_number="6.-.-.-"
                     /GO_function="GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854441.1"
                     /locus_tag="FED44_RS31635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31635"
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="WP_220499464.1"
                     /transl_table=11
                     /translation="MGTVVTRVSDLPKESMRERLADLQVLKESIHSGPSEEATARQHAK
                     GKLTVRERLDVLLDSGSFVEVEAFRQHRATGFGLEHRRPYTDGVVAGWGKVYGREVMVF
                     AHDFRMFGGALGEAHAEKIHKVMDLAEAAGVPLIGLNDGAGARIQEGVTALAGYGGIFR
                     RNVRMSGVIPQINVMLGPCAGGAAYSPALSDFVFMVQNTAQMFITGPDVVKAVTGEEIS
                     ADDLGGARVHGTLSGVAGFVCPDEVSCLEEVRYLLSLLPANNQELPPSITCTDPANRRN
                     DALLDLVPADPAISYDMSAVIEEIVDDGEFFEFHAGWAQNLICGLARFDGHVTGIIANQ
                     PAHLAGVLDIKASEKGARFIQMCDAFNIPLVSLVDVPGFLPGTDQEHGGIIRHGAKLLY
                     AYCAATVPRISMVLRKAYGGAYIVMDSRSIGADLSFAWPTNEIAVMGAEGAANVIFRRE
                     IAAAPDPVEAHAQRVEEYRRQLMHPYYAAERGLVDDVIDPSETRATLTRALGLLRAKQA
                     SGPRRKHGNPPM"
     gene            6636..6860
                     /locus_tag="FED44_RS31640"
                     /old_locus_tag="FED44_31640"
     CDS             6636..6860
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025192.4"
                     /locus_tag="FED44_RS31640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31640"
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="WP_117409931.1"
                     /transl_table=11
                     /translation="MGAELRVVAGNPTEEEICALVCALYAVAADRADGAEPGPVAGPKR
                     HRPRLRLPDYGSPRSWVACHRTDALTGVR"
     gene            complement(6942..8402)
                     /gene="mftF"
                     /locus_tag="FED44_RS31645"
                     /old_locus_tag="FED44_31645"
     CDS             complement(6942..8402)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /locus_tag="FED44_RS31645"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31645"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_138462967.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1e-21, bitscore:
                     69.9, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHDQITRDSDPRAPGRLPDGFTVRLNRRVRVRDGGRTLVGGAPT
                     RVMYLTPAAADLFDGRRLRVRDRVSALLADRLLEAGLADPVVGDLPGLDPSQVTVVVPV
                     RDRPAALDRLLSGIGDRHRVIVVDDASAAPEAVAEVAAAHGAVLVALAENVGPAAARNA
                     GLRRVTTPYVAFADSDVVLEPGTLPTLLRHFADPRVAVAAPRVLGLRLPGRARWILRYE
                     DARSSLDLGLDPGIVRPRAPVSWVPSAFLLARADALGHGFSAEMRVGEDVDLVWRLARA
                     GWRVRYEPAATVRHEHRTVVHDWLRRKAFYGTGADLLARRHGRDVAPAVLAPWSAAFVL
                     ALLAQRRWSLPAAALIFAGTAATMSAKVRRSEHPVRLALTLTADGAVAAVWQAAALLLR
                     HWWPLAAVGCLLSRRLRRAVAAVAVMDALTDLRRTGAHLDPVRYAVARRLDDAAYGSGL
                     WLGAIRGRSVRALLPDIRWKTRSSRPSA"
     gene            complement(8399..9223)
                     /locus_tag="FED44_RS31650"
                     /old_locus_tag="FED44_31650"
     CDS             complement(8399..9223)
                     /NRPS_PKS="Domain: PKS_KR (12-190). E-value: 1.1e-09.
                     Score: 30.4. Matches aSDomain:
                     nrpspksdomains_FED44_RS31650_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR04504.1"
                     /locus_tag="FED44_RS31650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31650"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_138462968.1"
                     /transl_table=11
                     /translation="MSQVSGEDGGFRVALVTGAARGIGAAVVRALCARGYRVVAVDSCA
                     ADGPARPAGVTYPLAADADLKSLADDHPGVVVPYIADVRDRDALARAAAVAADRFGRLD
                     AAVAAAAVMAGGLPLWETPDAHVRALWETDVLGVWNTAAACVPHMLAGPEPGACRFVAV
                     ASAAGSHGLFRLSAYTMAKHAVVGLVRGLAADLVGTGVTAAAVSPGSTRTPMLTATADV
                     YGLDGVEDFASSQLLRRLIEPAEIAETIAFCCSREGGVVNGSVISADGGFTP"
     aSDomain        complement(8654..9187)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FED44_RS31650_PKS_KR.1"
                     /evalue="1.10E-09"
                     /label="FED44_RS31650_PKS_KR.1"
                     /locus_tag="FED44_RS31650"
                     /protein_end="190"
                     /protein_start="12"
                     /score="30.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALVTGAARGIGAAVVRALCARGYRVVAVDSCAADGPARPAGVTY
                     PLAADADLKSLADDHPGVVVPYIADVRDRDALARAAAVAADRFGRLDAAVAAAAVMAGG
                     LPLWETPDAHVRALWETDVLGVWNTAAACVPHMLAGPEPGACRFVAVASAAGSHGLFRL
                     SAYTMAKHAVVGLVR"
     CDS_motif       complement(9113..9181)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_FED44_RS31650_0001"
                     /evalue="2.70E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="FED44_RS31650"
                     /protein_end="37"
                     /protein_start="14"
                     /score="13.6"
                     /tool="antismash"
                     /translation="LVTGAARGIGAAVVRALCARGYR"
     gene            complement(9220..10008)
                     /gene="mftE"
                     /locus_tag="FED44_RS31655"
                     /old_locus_tag="FED44_31655"
     CDS             complement(9220..10008)
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: protein motif:HMM:TIGR03964.1"
                     /locus_tag="FED44_RS31655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31655"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_138462969.1"
                     /transl_table=11
                     /translation="MRERGEGGLADLSWPALPSSPTVLVPLGSTEQHGPHLPLHTDTAV
                     AAAVARAAAARLLAVAPADPVLVAPPVAYGASGEHQDFPGTMSIGTAALRFLLVELVRS
                     LSRWAGRVVIVNGHGGNLDALAAATPLLREEGHDVCWAPCAAGPADAHAGHAETSLMLH
                     LAPRLVDMARAEPGNLAPLAEIMPELAARGVAAVSPSGVLGDPTTATAGEGRRLLDRMA
                     DDVAARVRHGRADHTGRLLPAPTPAPTPGPAAAAATASPA"
     gene            complement(10001..11224)
                     /gene="mftD"
                     /locus_tag="FED44_RS31660"
                     /old_locus_tag="FED44_31660"
     CDS             complement(10001..11224)
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293397.1"
                     /locus_tag="FED44_RS31660"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="FED44_31660"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_117409935.1"
                     /sec_met_domain="TIGR03966 (E-value: 3.9e-213, bitscore:
                     699.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAANAWFETVAEAQRRARRHLPKSVYSALLAGSERGRTYRDNTAA
                     FAELGFAPHVADLPAKRDLSTTVMGQQISLPVLISPTGVQAVHPDGEVAVARAAAARGT
                     IMGLSNFASKPVEEVVAANPQTFFQMYWMGTRESMAAHMERARAAGVKGLIVTLDWTFS
                     HGRDWGSPWIPERLDLKALARFAPQGLTRPRWLLGFARTGRLPDLTVPNVAEPGRPAPT
                     FFGAYGEWMRTPPPGWDDVRWMREQWDGPFMLKGISRLDDARRAVDAGVTAISVSNHGG
                     NNLDGTPATIRLLPGIARVVDGQAEVLLDGGVRRGGDVVKALALGARAVMIGRASLWGL
                     AANGQAGVENVLDILRGGIDSALLGLGRASIHDLEPGDVLVPPGFTREIGFTGEIGSTR
                     EIGSPGDA"
     gene            complement(11242..12849)
                     /gene="mftC"
                     /locus_tag="FED44_RS31665"
                     /old_locus_tag="FED44_31665"
     CDS             complement(11242..12849)
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03962.1"
                     /locus_tag="FED44_RS31665"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="FED44_31665"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_138462970.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 1.9e-29,
                     bitscore: 94.1, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 9.2e-25, bitscore: 80.3,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 2.8e-11, bitscore: 35.6,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 2.5e-15, bitscore:
                     49.0, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 8.1e-07, bitscore: 21.9,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDPLDEAWELSPRVALRPEPFGALAYHFGNRRLTFLKRPELVSV
                     LRDLSTEPDARAALTRAGVPAGRWPAYAEALRGLAGADMIRPRGRVESRSTAARNSTAQ
                     NSTAQNSTAQNSAVQDSPARSGTASGSLAKRFEYGLAAPICLTWELTYACNLSCVHCLS
                     SSGRRDPRELATAQCEAVIDELQAMQVFYVNIGGGEPTVRPDFWHLVQYAVNHQVGVKF
                     STNGTRLTPERARFLAATDYVDVQVSLDGATPEVNDRVRGPGSYETALRALDNLAAAGF
                     ADAKLSVVVTRENAGQLDEFKAIADERGATLRLTRLRPSGRGADVWDELHPTAAQQREL
                     YEWLLRAGEDVLTGDSFFHLAGYGESLPGLNLCGAGRVVCLIDPLGDVYACPFAIHDRF
                     RAGNVLSDGGFAAVWRESELFEDLRSPQTSGACTGCGFFDSCRGGCMAAKFFTGLPLDG
                     PDPECVQGHAADALAAAGRTVPRPSQDHSRRPGARRAGAGPVPVRIGRRPSAAPPVHPP
                     VHACAESPLAGFSPSSDV"
     aSDomain        complement(12595..12834)
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_FED44_RS31665_Mycofactocin_RRE.1"
                     /evalue="6.80E-30"
                     /identifier="RREFam016.1"
                     /label="FED44_RS31665"
                     /locus_tag="FED44_RS31665"
                     /protein_end="85"
                     /protein_start="5"
                     /score="94.1"
                     /tool="antismash"
                     /translation="DEAWELSPRVALRPEPFGALAYHFGNRRLTFLKRPELVSVLRDLS
                     TEPDARAALTRAGVPAGRWPAYAEALRGLAGADMI"
     gene            complement(12849..12947)
                     /gene="mftA"
                     /locus_tag="FED44_RS31670"
                     /old_locus_tag="FED44_31670"
     CDS             complement(12849..12947)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049342.1"
                     /locus_tag="FED44_RS31670"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="FED44_31670"
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_117409937.1"
                     /transl_table=11
                     /translation="MEETTVPAEATEELTAETLIEEVSIDGMCGVY"
     misc_feature    12978..12991
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.11"
                     /tool="antismash"
     misc_feature    complement(12979..12992)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.84"
                     /tool="antismash"
     gene            complement(13231..14010)
                     /locus_tag="FED44_RS31675"
                     /old_locus_tag="FED44_31675"
     CDS             complement(13231..14010)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541262.1"
                     /locus_tag="FED44_RS31675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31675"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_244302439.1"
                     /transl_table=11
                     /translation="MRREGDAVPKPVVGESARRRRRPTKNGVVLSEGLIVRTALRMLRE
                     HGPAGLTVRRLGIALGADPSTLYRYFRGVDDLTLAIGDMLVGQAMAGWSGGGDWRAGLR
                     DLGLRIHAAYLEHPQAAVLTASRISGKSHEIAADEAILGLLRGAGFPDREAVGVYHTFI
                     DMTLAFAALDAATLALPAEAREADEEIWRATYARLPRATHPNIAAVAPLLVARMNDSAY
                     PAALEMLLDSAASRLTRPRPAPRTARGGGRDRPDKGV"
     misc_feature    14078..14091
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.13"
                     /tool="antismash"
     misc_feature    14088..14101
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.49"
                     /tool="antismash"
     gene            14106..15329
                     /locus_tag="FED44_RS31680"
                     /old_locus_tag="FED44_31680"
     CDS             14106..15329
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888683.1"
                     /locus_tag="FED44_RS31680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31680"
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="WP_138462971.1"
                     /transl_table=11
                     /translation="MRVLLVGAGGVGTAITRIAARRSFLEHMVVADYDLSRAKAAVAAL
                     GETGDRFSACRADAGDEVAVAALLAEHRCDVLLNATDPRFVMPLFRAALAAGAHYLDMA
                     MSLSRPHPDRPHEECGVKLGDAQFETAAEWERAGRLALVGMGVEPGLSDVFARYAADEL
                     FDHIEEIGVRDGADLTVDGYGFAPSFNIWTTIEECLNPPVVYETGRGWFTTPPFSEPEV
                     FDFPEGIGPVECVNVEHEEVLLMPRWIDAERVTFKYGLGAEFIDTLRTLHLLGLDRTEP
                     VQVAAGGGTAVKVSPRDVVAACLPDPATLGERMRGKTCAGTWVKGTKDGGPREVYLYHV
                     VDNQWSMREYGSQAVVWQTAVNPVVALELMASGAWSGAGVLGPEAFPPRPFLDLLTEYG
                     TPWGMREQ"
     gene            complement(15357..15890)
                     /locus_tag="FED44_RS31685"
                     /old_locus_tag="FED44_31685"
     CDS             complement(15357..15890)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FED44_RS31685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FED44_31685"
                     /product="hypothetical protein"
                     /protein_id="WP_138462972.1"
                     /transl_table=11
                     /translation="MKTPVRTPAQTHRVRRSALAAALLAGALFAGGVLGPAGTPAQAAT
                     TITASLSITRAAPPGPGLMNVRVLVKVPMNLYDANGYLNNGARIELRFYGEDPVSDNLV
                     LGPITYVRSAVGLSATEEGIRLLTQWQEEPGSWLNEDDGLLDGVTDEVYVRARFVDGDG
                     VAIQARSNVVTGIF"
     gene            complement(15981..19151)
                     /locus_tag="FED44_RS31690"
                     /old_locus_tag="FED44_31690"
     CDS             complement(15981..19151)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1149: LuxR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /locus_tag="FED44_RS31690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31690"
                     /product="AAA family ATPase"
                     /protein_id="WP_138462973.1"
                     /transl_table=11
                     /translation="MQVHITMLGRFAVHVDGSVLPPGAWHRRQAAELVKLLALAPRRRL
                     HREEVIDALWPGVSPGQAAPRLHKAAHYARRALGATDGVVLRGESVGLLPDVPVVVDAV
                     RFAELAAAALADGGAAAAVAALEAYAGPLLPDDPYERWAQAHRDRLRLLYARLLRQAER
                     WDELVELDPANEEAHLALIRRHAERGDRRAALRQYERLDRALGRELGVAPGAEAVALRE
                     ALTADPAGRAGPAAPAVLVGREAQTRRFEETIAEVGRGRGRTVFLAGAPGIGKSFLARW
                     ALDRVAGMGWRTGAGAASRVEGAWPYAPVLEAVADLCRRHPTLLDGLDDRCRESIELAL
                     SGRDLDWSGDGGHQRLYVAVMELMRLASAGSGALLVVDDAHEADEAGLRLLHYLARSCA
                     SERVLLLLCHRRQPLTDTFEQVRSSLLTRGAAVELPLTPLGREETEALARASRPGLGPE
                     TGERIWHLSGGLPFAVVEAARVAGEGDAPGAVPGEVVLGLLDPRARAVLERVAIAGSAF
                     DTDEFIALSGATEDEAFDCLDAALAALVAERTASGYRFRHPLIREALLAGIPPHRQRRL
                     HRVCAERLAALNASPARVGHHLLAAGDAAAAVPYVLRAAQTEAAVGAFREALALVDSIR
                     HVCGEADRPRALALRADLLAAVGDAAAAAAYREAIEAAPPPRRRPLRARLAQMLVLAGD
                     LETAGTMLDELDLGGGPPDPTILLARGQLAYFRGDLELARTVSEQARGLVTAGEMDRQV
                     LDLVTLRGLVAHNRGEWYQQLRVELRRTRDEPSLATAIFDSHLCVAEYLLYGPTPYREV
                     IELAAALRETATRAGAMRAVAFATALIGEAALLSDDLDLAERELRESVDLHREIGAFAG
                     ESHSLQRLAELCLARGDKAAAGRLLNRALPLARWSNLAMHLMQRIFGTMIVAADGPREA
                     RAVVDRAWATLGAGDVCFFCHVMLAVPSAIACALGDARLHLATAERSAELWQGTSWQAA
                     ILEARAHIARAEGDHPAEARLLAQAADLFDDAGHPRDAARCRRSGATWEAARKESPAGE
                     KDAGRASS"
     gene            complement(19183..20379)
                     /locus_tag="FED44_RS31695"
                     /old_locus_tag="FED44_31695"
     CDS             complement(19183..20379)
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1119: halogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /locus_tag="FED44_RS31695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31695"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_138462974.1"
                     /transl_table=11
                     /translation="MESFDVAVVGARAGGAAAAMLLARQGLRVLLADRDRYGADTLSTH
                     ALMRGGVLLLSRWGLLDRIVAAGTPPVRQTRFHYGDETATVSIRPRLGVEALYAPRRSL
                     LDALLVDAAAAAGAEVRFGVTVTGLLREDSGRVAGVTGHDRRGTRVSVRARLTVGADGR
                     RSTVARAAGARTLRTGTGAGAVVYGYWTGLETSGYEWFYRPGHTAGMIPTNDGQVCVFA
                     GMPAALFRATAGEQGAAGTSGGGTSGARTGGGLGGRYHRMLAAATKGAGGRLAAAGPPL
                     RLRTWVGRPSFVREAQGPGWALVGDAGSFLDPLSSHGITDALRDAERLAREVAAAGGPG
                     SLSGTYAADRDRVIGPIYDAVDRIAAYGWDLNQVRRHLLELNSAMSAEVEMISAVGAG"
     gene            complement(20450..21022)
                     /locus_tag="FED44_RS31700"
                     /old_locus_tag="FED44_31700"
     CDS             complement(20450..21022)
                     /EC_number="1.11.1.-"
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /locus_tag="FED44_RS31700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31700"
                     /product="OsmC family protein"
                     /protein_id="WP_138462975.1"
                     /transl_table=11
                     /translation="MSTTQTRAPRNGVDTPTLFATLNAVKGDPELAKFRFRATNTWISG
                     THNRSVIRGFYGAGQEDTTRTDDFTYDADHPAVLVGGGNAPTPVEFLLHAIASCITAGV
                     ANIAAARGVTLHEVSSTVEGDIDLLGVLGLSERVRNGYQGIRVTFRISGDAPADVLRGL
                     VEQSRARSAVYDVLTNGTRVDIDVVAA"
     gene            21344..22093
                     /locus_tag="FED44_RS31705"
                     /old_locus_tag="FED44_31705"
     CDS             21344..22093
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630141.1"
                     /locus_tag="FED44_RS31705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31705"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_138462976.1"
                     /transl_table=11
                     /translation="MELADARRRRADRARQVADLLRCEIVRGGFDGGHLPLEAALAREF
                     GASRNTVRDALDLLRDEGLIARCPGVGTTVAAEKYPHGLYRLLGLAETLREHGEVTNEV
                     RAMGLIPPPAAVRQRLDLLEGEPVVYIERLRRLDGLPLSLDLTYLVRELGEPLFDADLV
                     HNDVFVLLERIAGRPLGAAELTIEAVNADAHTAAVLDAPRGAALLMVERLTHLSDGRPV
                     DLEFVRFRGDRLTMRGHLWRNTDDPGQ"
     gene            22077..22304
                     /locus_tag="FED44_RS31710"
                     /old_locus_tag="FED44_31710"
     CDS             22077..22304
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514073.1"
                     /locus_tag="FED44_RS31710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_31710"
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="WP_079312946.1"
                     /transl_table=11
                     /translation="MTLVNSRVDVPVTIDESLCIEGCTLCVDVCPLDSLAIHEVSGKAY
                     MHVDECWYCGPCADRCPADAVTVNIPYLLR"
     misc_feature    22758..22772
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
ORIGIN
        1 gcgggcgcct cgctggcttt ccaccgacct tcgcgacggc aaccagtcgc tggccagtcc
       61 gatgagcccg gcgcgcaaga tggccatgtt cgatctgctg gtccggatgg gctacaagga
      121 gatcgaggtc ggctttcccg tggcgagcga ggacgaccac gccttcctgc gcgagctcat
      181 cgagcgcgac ctgatccccg acgacgtccg cgtctccgtc ctggtgcagg cacgtgacga
      241 gttgatccgg cggaccgtgg agagcctcga aggcgctccc cgcgccacca tccacctgta
      301 caacgcgacg tcgccgttgt tccggcggct ggtgttcggg atgaccaggg acgagtgcaa
      361 ggacctggcc gtccagggca cgcgcctgat gatgaagtac gcggacaaga cgctgagcga
      421 ctgtgacctg ggcttccagt actctcccga gctgttcagc gacaccgagc tggacttcgc
      481 cctcgaggtc tgcgaggcgg tcatggacgt ctggcagccc ggccccggac gggacatcat
      541 cctgaacttc ccgtcgaccg tcgagcggag cacgcccaac gtcttcgccg accagatcga
      601 gtggctcgac cgcaacctgt cccggcggga gcacgtctgc ctgtccatcc acccgcacaa
      661 cgaccggggg accggcgtcg cctcggcgga gctggccatg atggccggcg cgcagcgcat
      721 cgagggctgc ctgttcggca acggcgagcg cgcgggcaac gtggacctgg tcacgctcgg
      781 catgaacctg ttcagccacg gcgtcgaccc gggcatcgac ttctccgacc tcaacgcgat
      841 ccgccgcgtc gtggaggagt gcaacggcat tccggtccac ccgcgccacc cctacggggg
      901 cgacctcgtc tacacctcct tctccggctc ccaccaggac gccatcaaga agggcttcga
      961 cgcgctggag cgggaggccg ctgcggtcgg cgccccgatg gacgagatgc cctgggacat
     1021 gccgtacctg cccgtcgatc ccgcggacgt gggccggacc tacgaggccg tggtgcggat
     1081 caacagccag tccggcaagg gcggcgtcgc ctacatcatg agcacctggt acggcaggca
     1141 cctgccgcgc gacctgcaga tcgacttcgc ccgggtggtg cagcggcggg ccgacagcga
     1201 aggcatggag atcaccccca gcggggtgcg ggagctgttc gagagcgagt acacgtgccc
     1261 gcccgggccg cccgtcttcg gaccggagcc gctcgccgtc gaactgcacg tggacggcgg
     1321 cgggttcgag gtcggcgcgg agcgcgccga cgcgctgcag gcactccggg tgagcctcgc
     1381 cgagtggggg atcgaggtcc gcgccgtcca ccggaccggt gtcggccagg gggaacagga
     1441 cgtcgcggcg ggggtcgcgg tctacgccga gtgccgcctg gggaaccgta cgcactgggg
     1501 cgtggggatc gagcgggacg ccgagaccgc cgcgctccgc gcggtgcagg ccgtcgtcca
     1561 ccgggccgcc cggctcgtcg agccctccgg caggaaccac cgcgaactgg gcgaggctca
     1621 cgggcgcccg cgcaggcggc ggctggccac caccgcgtga ccgcgcacga ccgacctccg
     1681 gccctcgtcc acgggccccg tacggaaggg cacgacgcga tgacgcgaaa gcagctgaag
     1741 aagagccggc cctcggggcg ccctgcggcc aaggacgaag cgcccgacct gcgtactccc
     1801 tccggccgcc cgctgaagtt ctgacgcgcc cgccgcctcc gccctgtcac gggcggggcg
     1861 gcgggtgtcg cgcgtcccgg cgggtcccgt ccctggcgcg tgaaatcgcc gtgaattccc
     1921 agctcacgag gtcatggcgg gggcgcggga gtgcgggata agcgcgcgat agcgatccaa
     1981 aagtggccgg cgagaccatc gtctccaaca agcaggcaag cgtccattcg taaggacgga
     2041 agctcagctg gagggggccc gcttgatgat gaacttgatt gagagagacg aggcactgac
     2101 ctcgttacag gagatgctgg cggccgcgct gacgggcaag ggcaaggtcg cgatgctcag
     2161 cggcaccgtc gccaccggga agagcgcact gctcgacgcg ttcgccgagc aggccgtgga
     2221 gctgggcgcc ctgcccgtcg ccgcgaccgc gtcccgcatg gagcaggacc tgccgctcgg
     2281 cgtgctcggc cagctcatcg aagaggcgcc gctggtggag gaggagcgcg tgcgcgcgct
     2341 cgcgctgctt gaggagggca agcagaccgc gatgagctcc gggctcgcct ccgagaccct
     2401 ggaccaggtg gacgcccaga tcgcgcacgc tctgtgcact gtgctgctgg agctgtcgga
     2461 gcgttatccc ctggtgatcg tggtcgacga cgtgcactac gccgaccgtc cgtcgttgct
     2521 gtgcctggcg tacctcgccc ggcgcgtacg cttcgcccgg atcatggtga tcttcagtta
     2581 ctccgactac gtgcgcagca ccgacacctt cttctggacc gacctgctgc gccagccgta
     2641 ctgcggggtc gtgcagctca acccgctgtc ccggcagggg gtgcacacgc tcgtgcgcga
     2701 gcgggcgggc gacgacgtcg cggtccgctt cgcggaggac tggtacgcgt tcagcgccgg
     2761 caacccgctg ctggccgccg ccctcatcga ggactacctc ggcgggccgc gggagaggga
     2821 cggcgagccg gtgaccgacg agccggtggt cggcgagcgg tacggccagg ccgtgctgac
     2881 ctgcctgcac cggggagagc ccgaactgct caggatcgcc cggtgcctgg cggtgctcgg
     2941 cgacaccgac tcgctcgacc ggctgctcgg cctggggcac acccagatca gcagggccat
     3001 gaacgccctg acggccggcg gcgtgctcag cgtgggccgg ttccggcacg agtcggcccg
     3061 tacggccgtg ctggccgagg tgggcctcga cgagcgccgg gacctgcacc gccgcgcggc
     3121 cgaactggcg cacgcggacg gcgcggccac gtcggtgatc gccgatcacc tcgtccaggc
     3181 cggcccgatc gacgcgccct gggtggtgcc ggtgctggag gacgcggcca gggacgcgct
     3241 gcgcgagggc cgcaccgagc gtggcgtgga ctacctgcgc ctcgcgtggc aggcgtgcac
     3301 cgacgagcgg cgccgcctca agatcgccac gatgctggtg cgtgccgagt ggcgcatcaa
     3361 ccccgaggcg cccgaccggc acctggccga cctcaccgag gcgatgcagc gcggtcacct
     3421 gcgcggcagc gacggcgtgg tcctggcccg cgcgctgctt tggcacggcc ggtacgacga
     3481 cgcgcaggac gtgatcgagc atctgaccac ggccggctac gagagcgacc ccgagacggt
     3541 gatggagctc aacatcgcgc gcccgtggct gcgcgccacc catcccgcct tcgcgtccaa
     3601 gctcggcgag ccgcgcgcgg acaccggaac cgccctggtg tcggtggccg cggggcgccg
     3661 tctggagacg gcgaccgcgc tcgcgcaggt gctcgtcaac ggcccgcacg agggcgccct
     3721 gatcgccgtc gagcgcatcc tgcggctcgc gcgcctggac gagatgtcga tggacaccgt
     3781 ggagtgcgcg ctgctcgcgc tcacctacgg cgggcaggcg gagaaggccg cgccgctgtg
     3841 cgacctgttc agcgtcgagg cgtcctcccg gcgcgccccg agccgccagg ccaggctctc
     3901 ggccatccgc gccgagatgg ccgtacggca gggcgacatg gtcagcgccg agcagtacgc
     3961 gcgggccgcg ttggagatca tgcccatcac gagctggggt gtcacggtgg gcagcccgct
     4021 cggcagcctg gtgatcgcgc tgaccgcgat gggccggttc gacgacgtgc acgacatcct
     4081 cgaccggccc gtgctggacg ctatgttcga gagccggtac ggcctgcact acctgtacgc
     4141 caggggccgc tacagcctgg cggtcgggca ggcggcgctg gccctgcggg acttccagct
     4201 ctgcggcgag ctgaccggca ggtggggcct ggacgtgccg ggcctgctgc cgtggcgggc
     4261 cggcgccgct gaggcctgcc tgcagatggg ccggtccgag caggcctccc ggctgatcga
     4321 ggaccagttg tcccgctgca actcccgcac gccgcgggag ctcggcatga cgatccgcct
     4381 gctggcggcc accagcgagc tgcggcaccg gccgatgctc ctgcgcaagt ccgccgacat
     4441 gctgcagaac ggcggtgacc gctacgagca ggcccgctcg ctcttcgacc tggcggaggc
     4501 ctatcgcggc ctgggcgagc accggagagt cggcatgatc atgattcggg cgcgggccct
     4561 gatgaaggag tgcggagccg agatcggcgg cgcgcaggcg gcggccgaga ccggcaagca
     4621 ggcctccccg gctctgagcg ggggcagcgc ggcgctgctg agcgaggccg agcgccgggt
     4681 cgcggtgctg gccgccgccg ggcacaccaa ccgggagatc gccggtgagc tctacctcac
     4741 ggtgagcacg gtggagcagc acctcacccg gatctaccgc aagctcgaca tcagcagccg
     4801 ggccgacctg cccgcggtcc tcgagttcgg cggggccgcc gtcggctgag tcgatcgtgg
     4861 tcccccggtg gccggcccgt agggggccgg ccaccgccgg accggaacac tgatatccgg
     4921 accgttttcc ggttctattc gggacgcgtt ttatcggcgc ggtcgccgtc ctttttaggg
     4981 gtgtcgtggc gttgatcggc ccccttaccc tgagggcacc cggttgtgcc gtcaggagga
     5041 atacatatgg ggaccgtggt gacgcgggtc agcgacctgc cgaaggaatc tatgcgggaa
     5101 cggctggcgg atttgcaggt gctcaaggag agcatccatt caggcccgag tgaggaggcg
     5161 accgcgcgcc agcacgccaa gggcaagctc accgtgcggg agcgcctgga cgtgctcctc
     5221 gacagcgggt ccttcgtgga ggtggaggcg ttccggcagc accgggccac cggtttcggg
     5281 ctggagcacc gcaggccgta caccgacggc gtggtggcgg ggtggggcaa ggtctacggc
     5341 cgcgaggtca tggtcttcgc ccacgatttc cggatgttcg gcggcgcgct cggtgaggcg
     5401 cacgccgaga agatccacaa ggtgatggac ctggccgagg ccgccggggt gccgctgatc
     5461 ggtttgaacg acggcgccgg ggcccggatc caggaaggtg tgaccgcgct tgccggttat
     5521 ggcggtattt tccggcgcaa tgtgcggatg tcgggggtga ttccgcagat caacgtcatg
     5581 ctcggcccgt gcgcgggggg agcggcgtac tcgccggctc tctccgattt cgtgttcatg
     5641 gtgcagaaca cggcccagat gttcatcacg ggcccggacg tcgtgaaggc ggtgaccggt
     5701 gaggagatca gcgccgacga cctcggtggt gcgcgggtgc acggcacact ctccggcgtg
     5761 gcggggttcg tctgcccgga cgaggtgtcc tgtcttgagg aagtccgcta tctgctgtcc
     5821 ctgctgcccg ccaacaacca ggaactgccg ccgtccatca cctgcaccga cccggcgaac
     5881 cggcgcaacg acgccctgct cgacctggtg ccggctgatc cggcgatctc ctacgacatg
     5941 tcggcggtga tcgaggagat cgtcgacgac ggggagttct tcgagttcca cgcgggctgg
     6001 gcgcagaacc tcatctgcgg gctggccagg ttcgacggcc acgtcaccgg catcatcgcc
     6061 aaccagcccg cccacctggc cggggtgctg gacatcaagg cctcggagaa gggcgcgcgc
     6121 ttcatccaga tgtgcgacgc cttcaacatc ccgctggtca gcctggtcga cgtgccgggc
     6181 ttcctgcccg ggaccgacca ggagcacggc ggcatcatca ggcacggcgc caagctcctc
     6241 tacgcgtact gcgccgcgac cgtgccgcgc atctcgatgg tgctgcgcaa ggcgtacggc
     6301 ggcgcgtaca tcgtgatgga ctccaggtcg atcggcgcgg acctgtcctt cgcctggccg
     6361 accaacgaga tcgcggtcat gggcgcggag ggcgcggcca acgtcatctt ccgccgggag
     6421 atcgccgccg cgccggaccc ggtcgaggcg cacgcccagc gggtggagga ataccgcagg
     6481 cagctcatgc acccctacta cgccgccgag cgcgggctgg tcgacgacgt catcgacccg
     6541 agcgagacca gggccaccct gaccagggcg ctcggcctgc tgcgggccaa gcaggcgtcg
     6601 gggccgcggc gcaagcacgg aaaccccccg atgtgatggg cgccgaactg cgggtggtcg
     6661 cgggaaatcc caccgaggag gagatctgcg cgctggtgtg cgccctgtac gcggtcgcgg
     6721 cggaccgtgc ggacggcgca gaacccgggc cggtcgccgg gccgaagaga caccggcccc
     6781 ggctgcgcct gccggactac gggtcgcccc ggtcctgggt ggcgtgccac cgaaccgacg
     6841 cgctcaccgg tgtccggtga cgccgcagcc gccgccccgg gtgctcccgg ggcggcggct
     6901 caccacgttc tccaagcggc cggtctcccg gcgaacggct tctaggcgga cggcctcgac
     6961 gagcgtgtct tccaccggat gtcgggcagc agggcgcgca ccgagcgccc gcggatcgcg
     7021 ccgagccaca ggccggaccc gtacgccgcg tcgtccaggc gccgggccac ggcgtagcgg
     7081 accgggtcga ggtgcgcgcc ggtgcgccgc agatcggtga gggcgtccat cacggccacg
     7141 gcggccacgg cccggcggag cctcctggac agcaggcagc cgacggcggc gagcggccac
     7201 cagtggcgga gcagcagcgc cgccgcctgc cagacggccg cgacggcacc gtcggccgtc
     7261 agcgtcaggg cgaggcgcac cgggtgctcg ctccgcctga ccttggccga catcgtcgcg
     7321 gccgtgcccg cgaagatcag cgccgcggcc ggcagcgacc accgccgctg cgccagcagc
     7381 gccagcacga aggccgcgct ccagggcgcc agcaccgcgg gggccacgtc gcggccgtgc
     7441 cgccgggcga gcaggtcggc tccggtgccg tagaaggcct tgcgccgcag ccagtcgtgg
     7501 accaccgtgc ggtgctcgtg ccgcaccgtc gcggccggct cgtagcgcac ccgccagccc
     7561 gcccgcgcca gccgccacac caggtcgacg tcctcgccga cgcgcatctc ggcgctgaag
     7621 ccgtggccga gggcgtccgc ccgcgccagc aggaacgcgc tcgggaccca ggagaccggc
     7681 gccctcgggc gtacgatgcc cggatcgagg cccaggtcga gggaggaccg cgcgtcctcg
     7741 tagcggagga tccagcgggc ccggccgggc aggcggagcc ccagcacgcg cggcgcggcc
     7801 acggccaccc ggggatcggc gaagtgccgg agcagggtcg gcagcgtgcc gggttcgagg
     7861 accacgtcgg agtccgcgaa ggcgacgtag ggcgtggtga cccggcgcag gcccgcgttg
     7921 cgggcggcgg cggggccgac gttctccgcc agcgcgacga gcaccgcgcc gtgcgccgcc
     7981 gcgacctcgg cgacggcctc cggggcggcc gacgcgtcgt ccaccacgat cacgcggtgc
     8041 cggtcgccga tgcccgacag cagccggtcg agcgcggcgg gccggtcgcg caccggcacg
     8101 accaccgtca cctgcgaggg gtcgagaccg ggcaggtccc ccacgaccgg gtcggcgagg
     8161 cccgcctcca gcagccggtc ggcgagcagc gcagagacgc ggtcgcggac ccgcagcctg
     8221 cggccgtcga acaggtccgc ggcggccggg gtgaggtaca tgacccgcgt cggcgcgcca
     8281 cccaccaggg tgcgcccgcc gtcgcggacc cggacacgcc ggttcagccg gacggtgaaa
     8341 ccgtccggaa gccggccggg cgcccggggg tccgagtcgc gggtgatctg atcgtgggtc
     8401 atggagtgaa gcctccgtcc gccgatatca ccgatccgtt gacgacgcct ccctcgcgag
     8461 agcagcagaa cgcgatggtc tcggcgatct cggcgggctc gatcaggcgg cgcagcagct
     8521 gggacgaggc gaagtcctcg acgccgtcca ggccgtacac atcggcggtg gcggtgagca
     8581 tcggcgtgcg ggtcgaaccg ggggagacgg ccgccgcggt cacccccgtg cccaccaggt
     8641 cggcggcgag gccgcgcacc agcccgacca ccgcgtgctt ggccatcgtg tacgccgaca
     8701 ggcggaacag cccgtgcgag ccggccgcag aggccaccgc gacgaaccgg cacgcccccg
     8761 gctcgggccc cgccagcatg tgcgggacgc acgccgcggc ggtgttccac accccgagca
     8821 cgtcggtctc ccacagggcg cgcacgtgcg cgtccggggt ctcccacagc ggcaggccgc
     8881 cggccatgac cgccgcggcg gccacggccg cgtccagccg gccgaagcgg tcagcagcca
     8941 cggcggcggc gcgggcgagc gcgtcgcggt cgcgcacgtc cgcgatgtac ggcaccacca
     9001 cgccggggtg gtcgtcggcc agtgacttca ggtcggcgtc ggcagcgagc gggtaggtca
     9061 ccccggcggg ccgggccggg ccgtccgccg cgcacgagtc gaccgcgacc acccggtagc
     9121 ccctggcgca cagcgcccgc acgacggcgg cgccgatccc gcgggccgcc ccggtcacca
     9181 gggcgacccg gaagccgccg tcctcgccgc tcacctggct catgccggag aggccgtcgc
     9241 ggccgcggcc gcgggtccgg gtgtgggtgc gggggtgggg gcgggcagga ggcggccggt
     9301 gtggtcggcc cgtccgtgcc ggacgcgggc cgccacgtcg tcggccatcc ggtcgagcag
     9361 gcggcgtccc tccccggcgg tggccgtggt cggatcaccg agcacgccgg agggtgacac
     9421 ggcggcgacg cccctggccg cgagctcggg catgatctcc gcgagcgggg cgaggttgcc
     9481 cggctccgcc cgggccatgt ccaccagacg cggggccaga tgcagcatga gtgacgtctc
     9541 ggcgtggccg gcgtgggcgt cggccggccc ggccgcgcag ggcgcccagc acacgtcgtg
     9601 gccttcctcg cgcagcagcg gcgtcgcggc ggcgagggcg tccaggttcc cgccgtgacc
     9661 gttcacgatc acgacgcgcc cggcccagcg ggacagggag cggacgagtt cgacgagcag
     9721 gaaccggagg gcggcggtcc cgatggacat ggtgcccggg aagtcctggt gctcgccgct
     9781 cgcgccgtac gcgacgggcg gcgccacgag gacggggtcg gccggagcca ccgcgagaag
     9841 gcgggcggcg gcggccctcg ccaccgccgc cgcgaccgcc gtgtccgtgt gcaggggcag
     9901 gtgcgggccg tgctgctcag tggagccgag cggcaccagc acggtcggcg aggacggcag
     9961 cgcgggccac gacaggtccg ccaggccgcc ttcgcccctc tcacgcatcg cccggagacc
    10021 cgatctccct ggtggagccg atctccccgg tgaacccgat ctccctggtg aagccgggcg
    10081 ggaccagcac gtcgccgggc tccagatcgt ggatcgacgc gcggcccagg ccgagcagcg
    10141 ccgagtcgat gccgccgcgc aggatgtcga gcacgttctc cacgcccgcc tggccgttcg
    10201 ccgccaggcc ccacagcgag gcgcgtccga tcatcacggc ccgcgcgccg agcgcgagcg
    10261 ccttgaccac gtcgcccccg cgccgtacgc cgccgtcgag cagcacctcg gcctggccgt
    10321 cgaccacccg cgcgatgccc gggagcagcc ggatggtcgc gggcgtgccg tccaggttgt
    10381 tgccgccgtg gttcgacacc gagatcgcgg tgaccccggc gtccaccgcg cggcgggcgt
    10441 cgtcgagccg ggagatgccc ttgagcatga acgggccgtc ccactgctcg cgcatccacc
    10501 ggacgtcatc ccagccgggt ggcggggtcc gcatccactc gccgtacgcg ccgaagaacg
    10561 tcggagcggg acggccgggc tccgccacgt tgggaacggt gaggtcgggc agccggccgg
    10621 tcctggcgaa gcccagcagc caccgcggcc gggtcaggcc ctgcggcgcg aagcgggcga
    10681 gcgccttcag gtccaggcgt tcggggatcc acgggctgcc ccagtccctg ccgtgggaga
    10741 acgtccagtc cagggtcacg atcagcccct tgaccccggc ggcgcgggcc cgctccatgt
    10801 gcgcggccat ggactcgcgg gtgcccatcc agtacatctg gaagaaggtc tgcgggttgg
    10861 cggccacgac ctcctcgacg ggcttgctcg cgaagttgct cagccccatg atcgtgccgc
    10921 gcgccgcggc ggcgcgtgcc accgcaacct ccccgtcggg atgcacggcc tgcaccccgg
    10981 tgggggagat gagcaccggc agggagatct gctgccccat cacggtggtg gacaggtcgc
    11041 gcttggccgg caggtccgcc acgtgcggcg cgaagccgag ttcggcgaac gccgccgtgt
    11101 tgtcgcggta cgtccggcct ctctccgaac cggcgagcag cgcggagtac accgacttcg
    11161 gcaggtggcg tcgcgctctg cgctgcgcct ccgcaaccgt ctcgaaccag gcgttcgcgg
    11221 ccacgagcta tcgtccttct gctacacgtc ggatgagggg ctgaacccgg cgagcgggct
    11281 ctccgcgcac gcgtgcacgg gtgggtgtac gggtggggcg gcggaggggc ggcgtccgat
    11341 gcgcaccgga accggcccgg ctccggcccg gcgggcgccg gggcggcggg agtggtcctg
    11401 gctgggccgg ggcaccgtac ggcccgccgc ggcgagcgcg tcggcggcgt gcccctgcac
    11461 gcattcgggg tcgggcccgt cgagcggcag gccggtgaag aacttggcgg ccatgcaccc
    11521 gccccggcag gagtcgaaga acccgcatcc ggtgcacgcg cccgaggtct gcggcgaccg
    11581 caggtcctcg aacagctccg actcccgcca cacggcggcg aacccgccgt cggagagcac
    11641 gttgccggcg cggaagcggt cgtggatcgc gaacgggcag gcgtacacgt cgcccagagg
    11701 gtcgatcagg cagaccaccc ggcccgcgcc gcacaggttc agccccggca gcgactcgcc
    11761 gtaccccgcg aggtggaaga acgagtcgcc ggtgagcacg tcctcgcccg cgcgcagcag
    11821 ccactcgtac agctcacgct gctgggccgc cgtcgggtgc agttcgtccc acacgtcggc
    11881 gccccgtccg gacgggcgca ggcgggtgag ccggagcgtc gccccgcgct cgtcggcgat
    11941 ggccttgaac tcgtcgagct ggcccgcgtt ctcccgggtc acgaccaccg acagcttcgc
    12001 gtcggcgaac ccggccgcgg cgaggttgtc gagcgcccgc agcgccgtct cgtacgaccc
    12061 cgggccgcgg acccggtcgt tgacctcggg ggtggcgccg tcgagcgaca cctgcacgtc
    12121 cacgtagtcc gtggcggcca ggaaccgcgc ccgctcgggg gtgagtcgcg tcccgttcgt
    12181 ggagaacttc accccgacct ggtggttcac ggcgtactgc accagatgcc agaagtcggg
    12241 ccgcacggtc ggctccccgc cgccgatgtt gacgtagaag acctgcatgg cctgcagctc
    12301 gtcgatcacc gcctcgcact gggcggtggc gagctcacgc gggtcacgcc gccccgacga
    12361 cgacaggcag tgcacgcacg acaggttgca ggcgtaggtg agttcccagg tcaggcagat
    12421 gggagcggcc aggccgtact cgaaccgctt ggccagcgac ccgctcgccg tccccgaccg
    12481 tgccgggctg tcctgtactg ccgagttctg cgccgtcgag ttctgcgccg tcgaattctg
    12541 cgccgtcgag tttcgcgctg ccgtgctccg gctctccacc cgcccgcgcg gcctgatcat
    12601 gtccgcgccg gcgaggcccc gcagggcctc cgcgtacgcg ggccaccggc cggcgggaac
    12661 gccggcacgg gtcagcgcgg cacgggcgtc gggctcggtg gagaggtcgc ggagcaccga
    12721 gacgagctcg gggcgcttga ggaaggtcag ccgccggttg ccgaagtggt aggccagggc
    12781 cccgaacggc tccggccgca gggccacccg gggagacagc tcccaggcct cgtccagggg
    12841 atcgttcatc agtagacgcc gcacatgccg tcgatcgaca cctcttcgat cagcgtctcc
    12901 gccgtgagct cctccgtcgc ctcggccgga acggtcgtct cctccatggc tgctccttcc
    12961 tcggggcgcg gaacctggtt ttttggaata agcgggtttt cggggataag ccgggtcttc
    13021 gggatattcg tgcggcgaga gccggaccgg ggcgtcctcg cggaatcgcc ggcgctctcg
    13081 agatgtgagg gaagggacta ccgccgcgac ggtctcgatt cgagcggcca cgattcgagc
    13141 ccgctcgacc tgagcgaact cgaatgaggc gactcgcctt tctgctgaaa caccaatcac
    13201 actaaaagga gtgataggcg tggaaaaccc ctagacaccc ttgtccgggc ggtcccggcc
    13261 cccgcctcgt gccgtccggg gtgcggggcg ggggcgcgtg agccgcgacg cggcgctgtc
    13321 gaggagcatc tccagggcgg cggggtaggc gctgtcgttc atgcgggcga ccagcagagg
    13381 ggcgaccgcc gcgatgttgg ggtgggtggc ccggggcagc cgggcgtagg tcgcccgcca
    13441 gatctcctcg tcggcttcgc gcgcctcggc gggcagcgcc agggtggccg cgtccagcgc
    13501 cgcgaaggcc agcgtcatgt cgatgaaggt gtggtagacg ccgaccgcct cacggtcggg
    13561 gaaccccgcg ccacgcagca gcccgaggat cgcctcgtcg gccgcgatct cgtgcgactt
    13621 gccgctgatg cggctggcgg tcagcaccgc ggcctgcggg tgttcgaggt aggcggcgtg
    13681 gatgcgcagg cccaggtcgc gaaggccggc gcgccagtcg ccgcctccgc tccagccggc
    13741 catcgcctgt ccgacgagca tgtcgccgat ggccagggtc aggtcgtcca cgcctctgaa
    13801 gtagcggtag agcgtgctgg ggtcggctcc cagggcgatg ccgagtcgcc gtacggtgag
    13861 gccggcgggg ccgtgctcgc gcagcatgcg cagcgcggtc cgcacgatca ggccctccga
    13921 gaggaccacc ccgttcttcg tcgggcggcg ccgccggcgc gccgactcgc ccaccaccgg
    13981 cttgggcacg gcgtccccct ctctcctcac cgcctacctt atgccaacgc cattgacgtt
    14041 cggggtgggg gtggcgttgg atcggcacat caggggcgtt tgtccaagaa agggatgttt
    14101 gtaccatgcg cgtgctgctt gtcggagccg gtggagtggg tacggcgatc acccggatcg
    14161 cggcccgcag atccttcctc gagcacatgg tcgtcgccga ctacgacctg tcccgtgcca
    14221 aggcggccgt cgccgcgctc ggggagacgg gggatcgctt cagcgcctgc cgggccgacg
    14281 cgggtgacga ggtcgcggtc gcggcgctgc tggccgagca ccgctgcgac gtcctcctca
    14341 acgccacgga cccgcggttc gtcatgccgt tgttccgggc ggcgctggcg gccggcgcgc
    14401 actacctgga catggcgatg tcgctgtcgc ggccccatcc ggaccgcccg cacgaggagt
    14461 gcggggtcaa gctgggcgac gcccagttcg agacggccgc ggagtgggag cgggcgggcc
    14521 ggctcgcgct ggtcggcatg ggcgtggagc cggggctgtc ggacgtgttc gcccggtacg
    14581 ccgccgacga actcttcgac cacatcgagg agatcggcgt gcgggacggc gccgacctca
    14641 ccgtcgacgg atacggcttc gcgccgtcgt tcaacatctg gaccacgatc gaggagtgcc
    14701 tgaacccccc ggtcgtctac gagaccggcc ggggatggtt caccaccccg cccttcagcg
    14761 agcccgaggt gttcgacttc cccgagggga tcggcccggt cgagtgcgtc aacgtcgagc
    14821 acgaggaggt cctgctcatg ccccgctgga tcgacgccga gcgggtgacc ttcaagtacg
    14881 ggctgggcgc ggagttcatc gacacgctgc ggaccctgca cctgctgggg ctggaccgca
    14941 ccgagcccgt ccaggtcgcc gccgggggcg gcaccgccgt caaggtgtcc ccgcgcgacg
    15001 tggtcgcggc gtgcctgccc gacccggcca cgctcgggga gcgcatgcgg ggcaagacgt
    15061 gcgcgggcac ctgggtcaag ggaaccaagg acggcggccc gcgtgaggtc tacctctacc
    15121 acgtggtcga caaccagtgg tccatgcgtg agtacggctc ccaggcggtg gtgtggcaga
    15181 ccgccgtcaa cccggtcgtg gccctcgaac tgatggcctc cggcgcctgg tccggcgccg
    15241 gagtcctggg tcccgaggcc ttcccgccgc ggcccttcct cgacctgctc accgagtacg
    15301 gcaccccctg gggcatgcgc gagcagtgac cccggcgcgg gccgtgcccg cctccgtcag
    15361 aagatgcccg tgacgacgtt cgacctggcc tggatggcga cgccgtcgcc gtcgacgaac
    15421 ctggcccgta cgtagacctc gtcggtgacg ccgtcgagaa ggccgtcgtc ctcgttcagc
    15481 cagctcccgg gctcctcctg ccactgcgtc agcaggcgga tgccctcctc cgtggccgac
    15541 agccccaccg ccgacctcac gtaggtgatc gggccgagga cgaggttgtc ggagaccggg
    15601 tcctcgccgt agaagcgcag ctctatccgc gctccgttgt tgaggtaccc gttggcgtcg
    15661 tacaggttca tggggacctt gaccaggacg cgcacgttca tcaggccggg cccgggaggc
    15721 gccgctctgg tgatgctcag cgacgccgtg atcgtggtcg ccgcctgtgc cggtgtgccg
    15781 gccgggccga gcactccgcc ggcgaacagc gcgccggcga gcagcgcggc ggcgagcgcc
    15841 gagcggcgaa cgcggtgcgt ctgcgcgggg gtcctcacag gggttttcac aggggtcttc
    15901 acgggcttgt tcgaggggat gcgcatgggt ctcctccgga tggcgcacgc cgggcgctct
    15961 gcccggcgac tccgcacacg ctaggaggag gcccttcccg catccttctc tccggccggc
    16021 gactccttcc gcgccgcctc ccacgtggcg ccgctccggc ggcagcgggc cgcgtcccgc
    16081 ggatgcccgg cgtcgtcgaa gaggtcggcc gcctgtgcca gcaggcgggc ctccgccggg
    16141 tggtctccct cggcccgggc gatatgggcc cgggcctcca ggatcgccgc ctgccaggaa
    16201 gtgccctgcc acagctcggc ggagcgctcg gcggtggcca gatggagccg cgcgtcgccg
    16261 agggcgcagg cgatcgccga tggcacggcg agcatcacat ggcagaagaa gcagacgtcg
    16321 ccggcgccga gggtggccca ggcgcggtcg acgacggccc gcgcctcccg ggggccgtcc
    16381 gccgcgacga tcatggtgcc gaagatgcgc tgcatgaggt gcatcgcgag gttcgaccag
    16441 cgcgccagcg gcagggcgcg gttgagcagg cggcccgcgg ccgccttgtc gccgcgggcc
    16501 aggcacagtt cggccaggcg ttgcagggag tgcgactcgc cggcgaacgc gccgatctcc
    16561 cgatgcaggt cgaccgactc gcgcagctcc cgctcggcca ggtcgaggtc gtcgctcagc
    16621 agggcggcct ccccgatcag cgcggtcgcg aacgccacgg cccgcatcgc gcccgcccgc
    16681 gtcgcggtct cccgcagcgc cgccgccagc tcgatcacct cccggtacgg cgtcgggccg
    16741 tagagcaggt actccgccac acagaggtgg gagtcgaaga tcgcggtggc cagggacggc
    16801 tcgtcacggg tccggcgtag ctccacgcgg agctgctggt accactctcc ccggttgtgc
    16861 gcgaccagcc cccgcagtgt gaccaggtcg aggacctgcc ggtccatctc gcccgccgtc
    16921 accaggccgc gcgcctgctc cgacaccgta cgcgccagct ccaggtcgcc ccggaagtag
    16981 gcgagctgac cccgggcgag caggatcgtc gggtcgggcg gtccgccgcc gaggtccagc
    17041 tcgtccagca tcgtcccggc cgtctccaga tcgccggcca gcaccagcat ctgggccagc
    17101 cgggcccgga gcggcctgcg ccggggcggc ggggcggcct cgatcgcctc ccggtaggcc
    17161 gccgccgccg cggcgtcgcc gacggcggcg agcaggtcgg cgcgcagcgc gagcgcacgc
    17221 gggcggtcgg cctcgccgca cacgtgccgg atcgagtcga ccagggccag cgcctcgcgg
    17281 aacgccccca ccgccgcctc ggtctgcgcg gcacggagca cgtacggcac ggccgccgcg
    17341 gcgtcccccg ctgcgagcag gtggtgaccg acccgcgccg gcgaggcgtt cagggcggcc
    17401 agccgctcgg cgcagacccg gtgcagccgg cgctgccggt ggggcgggat gccggcgagc
    17461 agcgcctcgc ggatcagggg gtgccggaac cggtagccgc tcgcggtgcg ctcggcgacc
    17521 agtgccgcca gcgccgcgtc caggcagtcg aacgcctcgt cctcggtcgc gccggacaac
    17581 gcgatgaact cgtcggtgtc gaacgcggag cccgcgatcg ccacccgttc gagcacggcc
    17641 cgcgcccgcg ggtcgagcag gcccagcacg acctcgcccg ggacggcgcc gggcgcgtca
    17701 ccctcgccgg ccacccgcgc ggcctcgacg acggcgaagg gcagcccccc ggagagatgc
    17761 cagatccgct cgcccgtctc cgggcccagc cccggccggg acgcccgtgc cagcgcctcg
    17821 gtctcctccc ggccgagggg agtcagggga agctccaccg ccgcgccccg cgtcagcagg
    17881 ctcgaccgca cctgctcgaa cgtgtccgtg agcggctgcc tgcggtggca gagcaacagg
    17941 agcacgcgtt cgctcgcgca gcttcgcgcg aggtagtgca gcaggcgcag gcccgcctcg
    18001 tccgcctcgt gcgcgtcgtc gacgacgagc agggcgccgc tgcccgcgga cgcgagccgc
    18061 atcagctcca tgaccgcgac gtagagacgc tggtggccgc cgtcgccgct ccagtcgagg
    18121 tcgcggccgg aaagcgccag ctcgatgctc tcccggcagc ggtcgtccag gccgtccagc
    18181 agggtggggt ggcgccggca gaggtccgcg accgcttcga gcaccggggc gtacggccag
    18241 gcgccctcca cccgcgacgc cgcgccggcg ccggtgcgcc agcccatgcc cgcgacccgg
    18301 tcgagcgccc accgggcgag gaacgacttg ccgatacccg gtgcgccggc caggaacacc
    18361 gtgcggcccc ggccccgccc gacctcggcg atcgtctcct cgaaccggcg ggtctgcgcc
    18421 tcgcggccca ccaggacggc gggagcggcc ggccccgccc gtccggcggg atcggcggtc
    18481 agcgcctcgc gcagggcgac cgcctccgcg cccggcgcga cgccgagctc ccggccgagg
    18541 gcccggtcga gccgctcgta ctgccgcagg gcggcccggc ggtcgccgcg ctccgcgtgc
    18601 cgccggatca gggcgagatg cgcctcctcg ttggccgggt cgagctcgac gagctcgtcc
    18661 cagcgctccg cctgccggag caggcgcgcg tacagcagac gcaaccggtc gcggtgggcc
    18721 tgcgcccatc gctcgtacgg atcgtcgggc agcaggggac cggcgtaggc ctccagcgcc
    18781 gcgacggccg ctgcggctcc gccgtcggcg agggcggcgg cggccagctc ggcgaagcgg
    18841 accgcgtcga cgaccacggg cacgtcggga agcagtccga cgctctcgcc gcgcagcacg
    18901 acaccgtccg tggcaccgag ggcccggcgg gcgtagtggg cggccttgtg cagccggggc
    18961 gcggcctggc cgggggagac gcccggccag agcgcgtcga tcacctcttc gcggtgcagg
    19021 cggcggcgcg gtgccagggc gagcagcttg accagctccg ccgcctggcg gcgatgccac
    19081 gccccgggcg ggagcacgct gccgtccacg tggacggcga accgtcccag catggtgatg
    19141 tgcacctgca tgtcctggca cctccggaca tgaaggtact cgtcagccgg cgccgacggc
    19201 actgatcatc tccacctccg cgctcatcgc cgagttgagc tccagcaggt ggcggcgcac
    19261 ctggttgagg tcccagccat aggccgcgat ccggtccacc gcgtcgtaga tcggaccgat
    19321 gacgcggtcc cggtcggcgg cgtacgtgcc ggacagtgat cccggtccgc ccgccgccgc
    19381 gacctcccgc gccagcctct ccgcgtcgcg cagcgcgtcg gtgatgccgt gggagctcag
    19441 cgggtccagg aaggagccgg cgtcgccgac cagcgcccac ccgggtccct gggcctcgcg
    19501 gacgaaactc ggccgcccca cccaggtacg caaccgaaga ggaggcccgg cggcggccag
    19561 cctgccgccg gcgcccttcg tggcggcggc gagcatccgg tggtagcgcc cacccaaccc
    19621 gcctcccgtc ctggcgccac ttgttccgcc gccggaggtc ccggcggcgc cctgttcccc
    19681 ggcggtggcc cggaagaggg cggcgggcat cccggcgaag acgcacacct ggccgtcgtt
    19741 ggtcgggatc atgcccgctg tgtgaccggg ccggtagaac cactcgtagc cgctcgtttc
    19801 caggccggtc cagtagccgt acacgacggc cccggccccg gtgcccgtac gcagcgtgcg
    19861 ggcaccggcg gcacgggcga cggtggaccg ccggccgtcc gcgccgaccg tgagccgggc
    19921 ccggacggac acacgggtgc cccggcggtc gtggccggtc accccggcga cccgccccga
    19981 gtcctcccgg agcaggccgg tgacggtgac cccgaaccgc acctcggccc cggcggccgc
    20041 ggccgcgtcc accagcagcg cgtcgagcag gctccgccgg ggcgcgtaga gcgcctccac
    20101 ccccaggcgg ggcctgatgc tgacggtggc ggtctcgtcg ccgtagtgga aacgggtctg
    20161 ccgcaccggc ggagtgccgg cggcgacgat ccggtcgagc agcccccacc gggacagcag
    20221 cagcacgcca ccccgcatca gggcgtgggt ggacagcgtg tccgcgccgt accggtcgcg
    20281 gtcggccagc agcacccgca gcccctgccg ggccagcagc atcgcggccg cggccccgcc
    20341 cgcgcgagcg ccgacgacgg ccacatcgaa actctccacg tcgtcttccc tctcccctcg
    20401 cgggcgcgcg tcggtgcgtg acccggccgg tctcaccgcg gacccgatct caggccgcga
    20461 ccacgtcgat gtccacccgc gtgccgttcg tcagcacgtc gtagaccgcc gacctggccc
    20521 gggactgctc gaccagcccg cgcagcacgt cggccggcgc gtcaccggag atccggaagg
    20581 tgacccggat gccctggtag ccgttgcgca cccgctccga caggccgagt acgcccagga
    20641 ggtcgatgtc gccctcgacg gtcgagctca cctcgtgcag cgtcacgccg cgggcggcgg
    20701 cgatgttcgc cacgcccgcg gtgatgcacg aggcgatggc gtgcagcagg aactccaccg
    20761 gggtcggggc gttgcccccg ccgaccagga ccgccgggtg gtcggcgtcg taggtgaagt
    20821 cgtcggtccg ggtggtgtcc tcctggccgg cgccgtagaa gccgcggatg acactgcggt
    20881 tgtgggtgcc gctgatccag gtgttggtgg cccggaaccg gaacttcgcc agttccggat
    20941 ctcccttcac ggcgttcagc gtcgcgaaga gcgtgggggt gtcgacgccg ttgcgcggcg
    21001 cacgggtctg tgtggtgctc acggtttcct cctccgccgc acggtcccgg ccgccctgcg
    21061 ggccgccggg cgacgtctac cggtgaccgg cgacgggagg acagtaggag gcccgccttc
    21121 ccgcgtcctt ccgcgagccg gacccggacc ttccccggct cgatctccgg ctcgcgcccg
    21181 agcccgccgt cgcctgccga ggcggcgggg aggtgccgga ggggtggcgg ttctcggctg
    21241 tcttcctgcg taccgccagg tcagcggtgc atggaggccg cctggggtgg cgtaatccgg
    21301 ccgttatcca tatattccta ccgggaagct aggcttcttt cccatggagc tggcggatgc
    21361 gaggcggcgg cgggcggacc gggcccgcca ggtggccgac ctgctgcggt gcgagatcgt
    21421 gcgcggtggt ttcgacggcg gccacctgcc cctggaggcc gctctcgccc gggagttcgg
    21481 ggcctcgcgc aacaccgtca gggacgccct tgacctgctc agggacgagg gcctcatcgc
    21541 gcggtgcccg ggagtgggca ccacggtcgc ggcggagaag tacccgcacg gcctgtaccg
    21601 gctgctcggc ctggccgaga cgctccggga gcacggcgag gtgacgaacg aggtccgcgc
    21661 catgggcctc atcccgcctc ccgccgcggt ccggcaacgg ctcgacctgc tcgagggcga
    21721 gcccgtcgtc tacatcgagc ggctgcgcag actcgacggg ctgccgctct cgctcgacct
    21781 gacctatctc gtacgagagc tcggcgagcc gctcttcgac gccgacctcg tgcacaacga
    21841 cgtgttcgtg ctcctggagc ggatcgccgg gcggccgctc ggcgcggcgg agctcacgat
    21901 cgaggccgtc aacgccgacg cccacaccgc cgccgtcctc gacgcgccgc gcggagcggc
    21961 gctgctgatg gtcgagcgcc tcacccacct ctccgacggc cgccccgtgg acctggagtt
    22021 cgtccggttc cgcggcgacc gtctcaccat gcgtggccat ctctggagga acaccgatga
    22081 ccctggtcaa tagccgcgtc gacgtgcccg tgacgatcga cgagtcgctg tgcatcgagg
    22141 gctgcacgct gtgcgtggac gtctgcccgc tcgactcact ggccatccac gaggtcagcg
    22201 gcaaggcgta catgcacgtg gacgagtgct ggtactgcgg cccctgcgcc gaccggtgcc
    22261 ccgctgacgc cgtcacggtg aacatcccct acctgctccg atagaaggac atccccccat
    22321 gcgcacaatc ttcgccaccg tggcgctgct ggccgcggcc gccggcgccc tctccgggtg
    22381 ctccgtctcc gcgggcgccg acgacggcaa ggagaccgtg gtcgtcggat atcagtcgaa
    22441 gaccatcaac acggtgacgg ccgggacgct gctgcgcggc ctcggctact tcgagaagcg
    22501 cctcggcggc aggtaccggg tcgagtggca ggactacgac accggcgccc cgatcaccgc
    22561 caagatggtg gccgggaaga tcgacatcgg gtcgatgggc gactacccgc tcctcatcaa
    22621 cgcctcgcgc acccagggcg tgtccgccgc ccggacccgg ctcgtgtcgg cgaccggcta
    22681 caacctgcgc ggcgggctca actcggtggt ggtcgccccg tcgtcgaagg cgcgcacgct
    22741 ggccgacctg aaaggtggga aggtgtcggc cagctccggg tcggcgggcc acggcacgct
    22801 cgtccaggcg ctggagcggg ccgggctcga ccccgccacc gacgtggag
//
