LOCUS       NZ_VANP01000008        11146 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Microbispora fusca strain NEAU-HEGS1-5 Scaffold8, whole genome
            shotgun sequence.
ACCESSION   NZ_VANP01000008
VERSION     NZ_VANP01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbispora fusca
  ORGANISM  Microbispora fusca
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Microbispora.
REFERENCE   1  (bases 1 to 395720)
  AUTHORS   Yu,B.
  TITLE     Isolation, diversity and antifungal activity of Actinobacteria from
            wheat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 395720)
  AUTHORS   Yu,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Northeast Agricultural University, School of
            Life Science, Mucai59, Harbin, Heilongjiang 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005864065.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:18:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,124
            CDSs (total)                      :: 7,053
            Genes (coding)                    :: 6,990
            CDSs (with protein)               :: 6,990
            Genes (RNA)                       :: 71
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3, 4 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 11 of 63
            Pseudo Genes (incomplete)         :: 57 of 63
            Pseudo Genes (internal stop)      :: 6 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:39:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 267363
            Orig. end                         :: 278509
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VANP01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11146
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11146
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11146
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6146](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6146)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1341..1649)
                     /locus_tag="FED44_RS22015"
                     /old_locus_tag="FED44_22020"
     CDS             complement(1341..1649)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FED44_RS22015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FED44_22020"
                     /product="hypothetical protein"
                     /protein_id="WP_138461886.1"
                     /transl_table=11
                     /translation="MTEPDIGIVGEWLHSYEEDTGDAEVYRPARHPFRPSRRPRRGLEF
                     RADGTFVELRPGPADRPRPVTGRWHARESGVRVVFPEGGRTDLTVLSCQDGVLTIAK"
     gene            complement(1654..2781)
                     /locus_tag="FED44_RS22020"
                     /old_locus_tag="FED44_22025"
     CDS             complement(1654..2781)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017393.4"
                     /locus_tag="FED44_RS22020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_22025"
                     /product="zinc metalloprotease"
                     /protein_id="WP_208759117.1"
                     /transl_table=11
                     /translation="MTEPTNARFPARDLCGTMLVHRRLLNESMTYRARRAAIETRALRY
                     EQGRRAPVRTEVLTIPVVVHVVHDRAVPADDIADEQIHSQIEALNRDFRAANPDVAKVP
                     DVWKALVADARVEFRLATRGPDGEPATGIVRVATDRHDFTVDDGVKSGATGGSDPWPSD
                     EYLNLWVCRLRGGLLGYAQFPGGPPQTDGVVILNTAFGTTGTAAHPFDGGRTATHEIGH
                     WLDLYHIWGDDDGACTSDDKVADTPNQADANYGLPAFPRVTCGNGPNGDMFVNYMDYTD
                     DAGMFMFTRGQAARMNACLEGVRASFLVSPAAKEAPAKEAPAKEERLTRAMPETWRPSH
                     DGDVERLLQDSERLTTEVLNDIRAAIGMVGGHTDG"
     gene            complement(2778..4895)
                     /locus_tag="FED44_RS22025"
                     /old_locus_tag="FED44_22030"
     CDS             complement(2778..4895)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /locus_tag="FED44_RS22025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_22030"
                     /product="NACHT domain-containing protein"
                     /protein_id="WP_138461887.1"
                     /transl_table=11
                     /translation="MERSRWHRRHHMWTSVVLGVLLLVLAVRLKLLVEDLELPEQGARN
                     ISMNVGVLMTAANSVIELMKGGLQFLSQWRTSRKPPSAPAQSDVDNAKDVLAGLVAEQW
                     RDETILRSLADPEPIPVPWRSTEHAELMDHPRLIAKDFVSFPSLSADLAGMAAELRALR
                     SRRLVILGGPGSGKTTLAVQLLMELLRTRLPGEPVPVLLSAARWDAEVHPRLQDWLADC
                     IAVDYPALTADEFGPDVAKTLTARGGVLPVIDGLDELPGQARAGILLALNHSMSDRDQL
                     ILTCRTEQFAESVDLIGDVLTAAAVIEPGEITAETAADYLQACLPPRPHPAWLHVLDAL
                     RKGIAPALAEVTATPLGLWLVRSAYVVSRQDPAPLLELGRADAARLYDHLCDRFIPALV
                     GSRTPAADADEPFRPRHSWDPEHVRHWLAYLSGQLLTPGEDTRNVAWWHLARYASTGTV
                     RLMVSVVSGVLVGLSIAAVAHAPRAGALFGLVSALAVLVMTRSWFTEPPGHAEFQVRGR
                     LSLLGRTLAEAVVAGLLGALVGGLMSHRAGGALKIALLSGLTYVLTVGLIRWVEHPTSS
                     TTARSPRSTWRADRNLTLIRALCGLGVGLTVAAVGLVAKLNPPAVLAGGLLLGPLFGLI
                     LGSHHAWLAYTLTIPRLARRGRLPLRAMCFLDDAHRLGLLRTEGPFYQFRNVELQRRLA
                     RDHIFERKDSP"
     misc_feature    complement(4251..4253)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4855..4869)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.78"
                     /tool="antismash"
     gene            complement(5001..6146)
                     /locus_tag="FED44_RS22030"
                     /old_locus_tag="FED44_22035"
     CDS             complement(5001..6146)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="FED44_RS22030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_22035"
                     /product="YcaO-like family protein"
                     /protein_id="WP_167521556.1"
                     /sec_met_domain="YcaO (E-value: 9.5e-40, bitscore: 131.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGEGREPAAWHCRLRGPDGVPAPSAEGMGKGRRAEARAGALFEAL
                     EHYLTGPALFDPAAVEPVEPARIAAGPLRADASAVLLDGRCAAPLACNRYQGPGGAVPV
                     PLFLSAPWYVEADAARLRERAGDAHDYTGLMRYSCNSGSAVGVTATEALLHALNETIER
                     DALSLLLVRAFLGTGERPSVVDPRTLPPELARAHAVAGQVTGAPVHLLDITSDLMVPTM
                     LAYVAPTPDRPHRRGAGTSLSPAHAAWRALTELVQTTVGERIQRRGDAERLARYPELLA
                     CGRFDLTEHLHAARTVPFPEDGARPGRPRDQLREVAARLGARGYAPLTRTVAVLPGWIT
                     AVHVVVPGLERFMLVVEGNLVLPGPRGRAAVRHAVRAGDGC"
     gene            complement(6235..6357)
                     /locus_tag="FED44_RS38170"
     CDS             complement(6235..6357)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FED44_RS38170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_259402575.1"
                     /transl_table=11
                     /translation="MGRGVDFARAESLDDVEQELTEAERERLREITEERDENAG"
     gene            complement(6419..7234)
                     /locus_tag="FED44_RS22035"
                     /old_locus_tag="FED44_22040"
     CDS             complement(6419..7234)
                     /GO_function="GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542018.1"
                     /locus_tag="FED44_RS22035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_22040"
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="WP_138461889.1"
                     /transl_table=11
                     /translation="MRRRLGLIVALVLTLLTLVVPAAAASRPLVVAHRGASAYAPENTV
                     AAFRLARDQGADMFELDVQETADHHLVLMHDTTLSRTTDVEKVFPGRSPWRVGDLTLAE
                     IRGLDAGSWFSRKYAGERVPTLGETLREMRGSGLGLLLEIKEPRLYPGVEARVAEEIGR
                     DTGWRGRIVVQSFDWESMRRFHRLVPGVPIGLLGTPPVADLPRLAAFADQINPPYAGVT
                     AGYVRRVHRLGMKVLTWTVDAPAAVRRLTGAGVDGVITNRPDVVREVAG"
     gene            complement(7372..8169)
                     /locus_tag="FED44_RS22040"
                     /old_locus_tag="FED44_22045"
     CDS             complement(7372..8169)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132006.1"
                     /locus_tag="FED44_RS22040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_22045"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_138461890.1"
                     /transl_table=11
                     /translation="MPTFEGAPHKARQMAESFGEDAERYDRTRPAYPEAMVERIVAASP
                     GPEVLDVGCGTGLAARQFRSAGCRVLGVEPDARMAAFARRGGIEVEVATFEAWHPADRT
                     FDAVVAGTAWHWVDPVAGAVKAAEVLRPGGRLAAFWHVFEPPPAVSESLAAVYRKVLPG
                     FPPQDGARPKSALDGYRPLLDKAADGIREVGAFGEPEEWRFDWQRFYTRDEWLDQLPTS
                     GLLTRLPPDTLSEMLAEVGAAIDAIGGGFTVAYVTVVVTAARL"
     misc_feature    8152..8168
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.0"
                     /tool="antismash"
     misc_feature    complement(8172..8186)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.18"
                     /tool="antismash"
     misc_feature    8218..8234
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.46"
                     /tool="antismash"
     gene            8402..8539
                     /locus_tag="FED44_RS35925"
     CDS             8402..8539
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FED44_RS35925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167521557.1"
                     /transl_table=11
                     /translation="MLEARGLEVPDDVRERIRRCQDLDQLRTWVRRAVTVTSAQELFTE
                     "
     gene            8668..9261
                     /locus_tag="FED44_RS22045"
                     /old_locus_tag="FED44_22050"
     CDS             8668..9261
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132005.1"
                     /locus_tag="FED44_RS22045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_22050"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_111701070.1"
                     /transl_table=11
                     /translation="MPTGVALRDVRTQLFDAAERVLLRDGPSGLTSRAVTTEAGCAKGV
                     LHRHFADFDAFLAELVLDGIARIDRQAAALRESAGTGTVAGNLTDVLTDLFGSVAVAVV
                     GLIISRDGLRARLRQARPGQGLPLLTEAAAMLAAYLAAERDLGRIAADADVDTLAPTLL
                     GASHLLFADRDGGPPQADAVHKMVNTVIAGVVRD"
     misc_feature    9016..9018
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9450..10229
                     /locus_tag="FED44_RS22050"
                     /old_locus_tag="FED44_22055"
     CDS             9450..10229
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /locus_tag="FED44_RS22050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_22055"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_138461891.1"
                     /transl_table=11
                     /translation="MNDDDRLILRRTFTEDAALYDRTRPGYPAAMFEHIPEGSRVLEIG
                     CGTGQATVALAGRGCAVTAVELGAEMAALARRKLARFTGVEVVNAAFEEWPLPPERFDV
                     VFAATSFHWLDPATRVSRSADALRADGLLAVVSTEHVAGGSEAFFAEVQRCYERWDPAT
                     PPGLRLTPANDIPYDSGEIDDSGRFGPVRFHRYEWDHAYTTREYLDLLNTYSGHRALPD
                     EARTGLLGCIAALIDGRYGGRIVKRYLTQLALARKLG"
ORIGIN
        1 gatcgtgggc agtcccgccc gggtcagccc gccgttcagg cccgccgtcc agtaacggcg
       61 caggtgctcg gggtcgcgtc cctgctggcc tcggccgtgc aggaacacca ggtgcagggc
      121 acccccgaac gccgcggagt ccgcggagag ccccgtcgtg ccgggctcgt caggagacac
      181 cgtggtggcc tctgacgagc cccgcatgac cggccgcgtg gccgcggact ccgccggccc
      241 cgccggcgcc gtcacgggtg acgcggtggg agccgaccct gcggcgcggc cgtcctggag
      301 ccccgactcc ggccccatct ccgcgagcag cgccgtccac gcgccgtcga gccgcagcgt
      361 gaccaggtgc cgcaggatcg agctgacccg cacgccctcg ttggacaccc agtccacggc
      421 gtcgtcgccg tcaccggcct gccagacctg gccgtcgcga cggaggatgc ggtcctggtc
      481 gtcggtcctc ggcacgccgc catggtggag cgccgtcacc tcccactggt cgttgtacac
      541 gggcgagccc gaactgcccg gctccgtgtc ggtctggtat tgcaggaagt cgtccagacg
      601 gacctccagc cggttctccc ggatggagat ctccttcaac cggccgccgg gatggccgac
      661 gatgttcacc ggctcgccga tgacgagctt gcccggctgc gcgctgagcg tgttccagcc
      721 gaagatctcc cccgccggcc gtccgccggc ctcgggagcg accaggacga gggcgaagtc
      781 gaggccctcg tcggccacga agaacgcgtc cggatcgagg gtgaaccgct tggccacctc
      841 aggcgtgttg tccacgccga cctgggcgtc gaactcggcg aacgcctgcc gtacggcctc
      901 cgggcagggg aggacgtggt ggttggtcag cagcagccgg ggcgagacca ggaagccggt
      961 accgatcggc agctcccggc cgttctcgct caccgagatc ctggcgacgg tgcgggccgc
     1021 ccgggccccg cgcggcagga agctccacgc ctggaggtcc ttggcgacgc cgaggatgcg
     1081 ctcgtgcatc gcgggccggt ccaggcggac gccccgggtg gcctccatca ctgcctcgac
     1141 cggcactccc tgacgctgga cgagccgggc ggcacgggcc gcgagcaggt ccggcgagtc
     1201 ggggaaggcg tcgccctcac tctgcctgcg gcgcacctcg tcccggaccg tgccgctgcg
     1261 gcgataccgt acggctgccg ccctggcgtg gcgcaggccc ttctcccgga cctcgtcggc
     1321 gatgtccacg gcgtctcccg tcacttcgcg atcgtcagca cgccgtcctg gcaggacagc
     1381 acggtgaggt cggtgcgccc gccttcgggg aagacgacgc gcacgccgct ctcccgcgcg
     1441 tgccagcggc ccgtcaccgg ccgcgggcgg tcggcggggc cggggcggag ctcgacgaac
     1501 gtgccgtccg cccggaactc caggccccgg cgcggacggc gagagggccg gaacggatgg
     1561 cgcgcggggc ggtagacctc cgcgtcgccg gtgtcctcct cgtaggagtg cagccactcc
     1621 cccacgatgc cgatgtccgg ttccgtcacg ggctcacccg tccgtgtggc cgccgaccat
     1681 gccgatcgcg gcgcggatgt cgttcagcac ctcggtggtc agccgctcgc tgtcctggag
     1741 cagccgctcc acgtccccgt cgtggctggg acgccacgtc tccggcatcg cccgcgtgag
     1801 gcgctcctcc ttggcgggcg cctctttcgc gggtgcctcc ttcgccgccg gcgagacgag
     1861 gaaagaggcc cgcacgccct ccaggcaggc gttcatccgc gcggcctgcc cccgggtgaa
     1921 catgaacatg ccggcgtcgt cggtgtagtc catgtagttc acgaacatgt cgccgttcgg
     1981 gccgttgccg caggtgacgc ggggaaaagc cggcagcccg tagttcgcgt ccgcctggtt
     2041 cggcgtgtcg gcgaccttgt cgtcgctcgt gcaggcgccg tcgtcgtctc cccagatgtg
     2101 gtagaggtcc agccagtggc cgatctcgtg ggtcgccgta cggccgccgt cgaaggggtg
     2161 ggccgccgtg cccgtcgtcc cgaaggccgt gttgaggatg acgacgccgt cggtctgcgg
     2221 cgggccgccg ggaaactggg cgtacccgag caggcccccg cgaagccggc acacccacag
     2281 gttcaggtac tcgtcgctcg gccacgggtc cgaccctccg gtggcgcccg acttcacgcc
     2341 gtcgtccacg gtgaagtcgt gccggtcggt ggcgacgcgc acgatccccg tcgcgggctc
     2401 gccgtcgggc ccgcgcgtgg ccaggcggaa ctccacccgg gcgtccgcca ccagggcctt
     2461 ccacacgtcc ggcaccttcg ccacgtccgg attcgccgcg cggaagtccc ggttgagcgc
     2521 ctcgatctgg ctgtggatct gttcgtcggc gatgtcgtcg gccggcacgg cccggtcgtg
     2581 caccacgtgc acgacgaccg ggatggtcag cacctcggtc cggacggggg cgcgccgccc
     2641 ctgctcgtac cgcagggccc gggtctcgat cgccgcgcgc cgcgcccgat aggtcatgct
     2701 ctcgttgagc agcctgcggt gcaccagcat cgtcccgcac aggtcgcggg ccgggaaccg
     2761 ggcgttcgtc ggttcggtca tggcgagtcc ttacgctcga atatgtggtc acgcgccagg
     2821 cgccgctgca actcgacgtt gcggaactgg tagaacggcc cctcggtgcg cagcaggccc
     2881 aggcggtggg cgtcgtcgag gaagcacatg gcgcgcagcg gcagccgtcc gcgcctggcg
     2941 agccgcggga tcgtcagcgt gtaggcgagc caggcgtggt ggctgccgag tatgagcccg
     3001 aacagcggcc cgagcagcag gccgcccgcg agcacggccg gcggattcag cttggccacc
     3061 aggccgacgg ctgcgaccgt cagaccgacg cccagcccgc acagcgcccg gatgagcgtg
     3121 aggttgcggt cggcgcgcca ggtcgaccgg ggggagcgcg ccgtcgtgct gctggtcgga
     3181 tgctcgaccc accggatcag gccgacggtg agcacgtatg tgagcccgga cagcagcgcg
     3241 atcttgagcg ccccgccggc cctgtggctc atcagcccgc ccacgagcgc gcccagcagg
     3301 ccggccacga cggcttccgc cagcgtgcgg ccgagcaggg acagccgtcc gcgcacctgg
     3361 aactcggcgt gaccaggcgg ctcggtgaac cacgatctcg tcatgaccag cacggcgagc
     3421 gcggacacca ggccgaacaa cgctccggcg cgcggcgcgt gcgccaccgc cgcgatcgag
     3481 aggcccacca ggaccccgct gacgacgctg accatgagcc ggaccgtccc ggtggaggcg
     3541 taccgcgcca ggtgccacca ggcgacgttg cgcgtgtcct ccccgggggt caggagctgg
     3601 cccgacaggt aggcgagcca gtgccgcacg tgctccgggt cccaggagtg ccggggccgg
     3661 aacggctcgt ccgcgtccgc ggcgggggta cgcgagccga cgagggcggg gatgaaccgg
     3721 tcgcacaggt ggtcgtagag acgggccgcg tccgcccgcc cgagttcgag cagcggcgcg
     3781 gggtcctggc ggctgacgac ataggcggag cggacgagcc agagccccag cggggtcgcg
     3841 gtgacctcgg ccagggcggg cgcgatcccc ttcctcagcg cgtcgagcac gtgaagccag
     3901 gcggggtgcg gcctgggcgg caggcacgcc tggaggtagt cggcggccgt ctcggcggtg
     3961 atctcccccg gctcgatgac cgccgccgcg gtgagcacgt ctccgatcag gtccacggac
     4021 tcggcgaact gctccgtacg gcaggtcagg atcaactggt cgcggtcgct catcgagtgg
     4081 ttgagcgcca ggaggatccc ggcccgcgcc tgtcccggca gttcgtccag gccgtcgatg
     4141 accggcagga ccccgccgcg cgccgtcagg gtcttcgcga cgtcaggacc gaactcgtcc
     4201 gccgtcagcg ccgggtagtc gacggcgatg cagtcggcca gccagtcctg taatcgggga
     4261 tggacctcgg cgtcccagcg tgccgccgac agcagcacgg gaacgggctc accgggcagc
     4321 cgcgtccgga gcagttccat cagcagttgc acggcgaggg tcgtcttgcc cgaccccggg
     4381 cctccgagga tgaccaggcg gcggctgcgc agcgcccgga gttcggccgc cattccggcg
     4441 aggtccgcgc tcaggctggg gaagctgacg aagtccttgg cgatcagccg cgggtggtcc
     4501 atgagctcgg cgtgctccgt agacctccac ggcaccggga tgggctcggg atcggccagc
     4561 gaccgcagga tcgtctcgtc cctccactgc tcggccacga gaccggcgag gacgtccttg
     4621 gcgttgtcga cgtcgctctg ggcgggggcc gacggaggct ttctggacgt gcgccactgg
     4681 gacaggaact ggaggccccc tttcatcagc tcgatgacgc tgttcgccgc ggtcatgagc
     4741 accccgacgt tcatcgatat attgcgggcc ccctgttccg gcagttccaa atcctcgacc
     4801 aacaacttga gccgcaccgc cagaaccagg agcaggaccc ccagcaccac cgaggtccac
     4861 atgtggtgcc gtcgatgcca cctcgacctc tccacaaggc catcatgcga accgctctgc
     4921 cggaagcgta agaagggaat ccgcccagtg tgggaaagtg ctcgtacacc cgcgcgcggg
     4981 gaggtcaagc ccttggtaga tcaacacccg tctccggccc gtacggcatg ccgcacggcg
     5041 gcccgtcccc gggggccggg caggacgagg ttgccctcca cgaccagcat gaagcgctcc
     5101 aggcccggca cgacgacgtg cacggccgtt atccaaccgg ggagaacggc caccgtacgc
     5161 gtgagcggtg cgtagccgcg cgcgccgagg cgggcggcca cttcgcggag ctggtcccgg
     5221 ggacggcccg gccgcgctcc gtcctcgggg aagggaaccg tacgggccgc gtgcagatgc
     5281 tcggtgaggt cgaaacgccc gcacgccagc agttcgggat accgggcgag ccgctccgcg
     5341 tccccccggc gctggatgcg ctcgcccacg gtggtctgca caagctcggt gagcgcccgc
     5401 catgcggcgt gagcggggct cagggaggtt ccggctccgc gccggtgcgg gcggtccggc
     5461 gtcggcgcga cgtaggcgag catggtgggg accatcaaat cgctggtgat gtcaagcagg
     5521 tggacgggcg ctccggtgac ctgaccggcc acggcgtggg cgcgggcgag ctcgggcggc
     5581 agggtgcgcg gatcgacgac cgacggccgt tcgcccgtgc cgaggaaggc gcgcaccagc
     5641 agcagggaga gcgcgtcgcg ttcgatcgtc tcgttgaggg cgtgcagcag cgcctccgtc
     5701 gcggtgacgc ccacggccga tccgctgttg cagctgtacc tcatcagacc ggtgtagtcg
     5761 tgggcgtcac cggcccgctc gcgcagccgc gccgcgtccg cctcgacgta ccagggggcg
     5821 gacaggaaca ggggcaccgg gacggcgccc ccgggtccct ggtaccggtt acaggccagc
     5881 ggcgcggcgc atcggccgtc gagcaggacg gcggacgcgt ccgcccgcag cggcccggcc
     5941 gcgatcctgg ccggttcgac gggttccacc gccgccgggt cgaacagcgc cgggcccgtc
     6001 aggtagtgct ccagcgcctc gaacagggcg ccggcacggg cctccgcgcg ccgtcccttg
     6061 cccatgccct ccgccgacgg cgccgggaca ccgtccggcc cccgcaggcg gcagtgccag
     6121 gcggccggct cacggccctc gcccatgtcc accaggtcgg gcgtcaggcc cagatgcgcg
     6181 agggccgcac cggcccggcg tacggcctcc ggaagcggga cgacccgctc cgcgctaacc
     6241 ggcgttctcg tcgcgctcct cggtgatctc gcgcagtcgt tcgcgttcgg cctcggtgag
     6301 ctcctgctcg acgtcgtcca gcgactcggc tctggcgaag tccacgccgc gtcccatggg
     6361 ctgctccctt caacaggcgg gagggacatc tgcccgcgtc cgggtcaggc caacaccatc
     6421 aaccggccac ctcccgtacg acgtccggcc tgttggtgat gacgccgtcc acacccgcgc
     6481 cggtcaggcg gcgtacggcg gcgggcgcgt ccaccgtcca cgtgaggacc ttcatcccca
     6541 gcctgtggac gcggcggacg tagccggccg tgacaccggc gtacggcggg ttgatctggt
     6601 cggcgaacgc cgccaggcgg ggcaggtccg cgaccggcgg cgtgcccagc aggccgatcg
     6661 gcaccccggg cacgaggcgg tggaaccggc gcatcgactc ccagtcgaac gactgcacca
     6721 cgatccggcc ccgccagccc gtgtcacgcc cgatctcctc ggccacccgg gcctcgactc
     6781 ccgggtagag ccggggctcc ttgatctcca gcagcaaccc gagcccgctg ccgcgcatct
     6841 cgcgcagcgt ctcgccgagc gtcggcaccc gctctcccgc gtacttccgg gagaaccacg
     6901 agccggcgtc cagcccgcgg atctcggcca gcgtgaggtc gccgacccgc cacggcgacc
     6961 ggccgggaaa gaccttctcc acgtcggtcg tacggctgag cgtcgtgtcg tgcatcagca
     7021 ccaggtgatg gtccgcggtc tcctgcacgt cgagttcgaa catgtcggcg ccctggtcac
     7081 gggccagccg gaacgcggcg acggtgttct cgggcgcgta cgccgacgcg ccgcgatgag
     7141 cgaccaccag cggccgcgac gccgccgcgg ccggaacgac gagggtgaga agggtcagga
     7201 cgagtgcgac gataaggccg agtcgccggc gcatggttct cctcgggtgt cgggaagcgt
     7261 cctacgaccc gaacctgacc acgcgccatt acaccgagtt gagttcccga tggcggccgg
     7321 gtgccgtctc gatgagcatc acgtccgtct ccggacattt cgccgtacgg gtcagaggcg
     7381 cgcggcggtg accaccaccg tgacgtaggc gacggtgaag ccgcctccga tcgcgtcgat
     7441 cgcggccccg acctcggcga gcatctccga cagcgtgtcc ggcgggagcc gggtcagcag
     7501 gccggacgtg ggcaactggt ccagccactc atcgcgcgtg tagaaccgct gccagtcgaa
     7561 ccgccactcc tcgggctcgc cgaacgcgcc cacctcccgg atgccgtcgg cggccttgtc
     7621 cagcagcggc cggtatccgt cgagggccga cttcggacgc gcgccgtcct gtggcgggaa
     7681 gccgggcaac accttccggt agaccgcggc gagggactcc gacacggcgg gcggcggctc
     7741 gaacacgtgc cagaacgccg ccagccggcc accgggacgc agcacctcgg cggccttgac
     7801 cgcgccggcg accggatcga cccagtgcca ggccgtcccg gcgacgaccg cgtcgaacgt
     7861 ccggtcggcg gggtgccagg cctcgaacgt cgccacctcg acctcgatcc cgccgcggcg
     7921 tgcgaaggcg gccatccgcg cgtcgggttc gacgccgagc acccggcagc ccgccgaccg
     7981 gaactgccgt gccgcgaggc cggtgccgca gccgacgtcg aggacctcgg gcccgggact
     8041 ggcggcgacg atccgctcca ccatggcctc gggataggcg ggccgggtgc ggtcgtaccg
     8101 ttcggcgtcc tcaccgaacg actccgccat ctgcctggcc ttgtggggtg caccttcaaa
     8161 agtgggcatg tgcccactta tagtgggcac atgcccactc gtcaacggcg ttcacccttg
     8221 gagttgtttt cgaaccgacc cgcccttcac ggccgcccgg gtgggaagta ttcaactcgc
     8281 tacccatagt gacgaccaag cgtctggagg agatggtgac caccattcag gacttcgagc
     8341 acctcggcga gaagcacttc tcccactggc tcgccaaggc gagagcgacg cgatcctcaa
     8401 cgtgctggag gcccggggcc tggaggttcc cgacgacgtc cgcgagcgga tccgccgctg
     8461 tcaggatctg gaccagttgc ggacctgggt acgccgggcc gtcaccgtca cctcggccca
     8521 ggagttgttc accgagtagc agacccggcg acgacccgac cttcgtatcc gtccacggtg
     8581 gtggagtcgc acactcggtc ctccagcttc gaagaccgag gaggcggcac cggtaaagtc
     8641 gatcgtggcg gagaggaggg acgaacggtg ccgacgggag tggccctccg cgacgtacgc
     8701 acgcagttgt tcgacgcggc cgagcgcgtg ctgctcaggg acggaccgag tgggttgacc
     8761 agccgggcgg tcaccaccga ggcgggctgc gccaagggtg tcctgcaccg gcacttcgcc
     8821 gacttcgacg ccttcctcgc cgagttggtg ctcgacggca tcgccaggat cgaccggcag
     8881 gccgccgctc tgcgcgagtc cgccgggacc ggcaccgtcg ccggcaacct caccgacgtg
     8941 ctgacggacc tgttcggttc ggtcgccgtc gcggtagtcg gcctgatcat ctcccgggac
     9001 ggcctgcgcg cccggttacg tcaggccagg cccggccagg gccttccact cctgacggag
     9061 gccgcggcca tgctcgccgc ctacctcgcc gccgagcgcg atctgggccg gatcgcggcg
     9121 gacgccgacg tcgacacgct cgctcccacg ctgctcggcg cctcacacct gctgttcgcc
     9181 gaccgcgacg gcggtccgcc ccaggccgac gccgtgcaca agatggtgaa caccgtcatc
     9241 gccggtgtcg tacgcgactg agccgcgaac cctcccgccc tgcgcgccgt cggcacagag
     9301 cgcctcgggg ccccggccgg gccggtgcag ccgatcaagg tgccacccgg aataaccacg
     9361 tgatcgtcag gagcgtggcc ggtaacgtga cctggtgtga cgaggccggc gctcttcaac
     9421 cgcgaaaccc gttgccctgg tagccgggcg tgaacgacga cgaccggctg atactgcgcc
     9481 gaaccttcac cgaggacgcc gccctgtacg accggacgcg gcccggctac ccggccgcga
     9541 tgttcgagca cattcccgaa ggcagccggg tgctggagat cggttgcggc accggtcagg
     9601 ccacggtcgc cctcgccggg cgtggctgcg cggtcacggc ggtcgaactg ggggccgaga
     9661 tggcggcgct cgcccgccgc aagctcgccc ggttcaccgg cgtggaggtg gtcaacgcgg
     9721 ccttcgagga gtggccgctg ccgccggagc ggttcgacgt cgtcttcgcc gccaccagct
     9781 tccactggct cgacccggcc acacgcgtga gcaggtcggc cgacgcgttg cgggcggacg
     9841 gcctgctggc cgtcgtctcg accgagcacg tcgccggtgg cagcgaggct ttcttcgccg
     9901 aggtgcagcg ctgctacgag cggtgggacc ccgccacgcc gcctggtctg cggttgacgc
     9961 ccgcgaacga catcccgtac gacagcggcg agatcgacga ctccggccgc ttcgggccgg
    10021 tgcggttcca tcgctacgag tgggaccacg cctacacgac gcgggagtat ctcgacctgc
    10081 tcaataccta ctccggccac cgcgccctgc ccgacgaggc ccgcacggga ctgctcggct
    10141 gcatcgcggc tctcatcgac ggccgttacg gcgggcgcat cgtcaagcgt tacctcaccc
    10201 agctcgccct cgcacggaag ctcggctgac ggtcagccgg gcggcgcggt gaaggcggct
    10261 tcgagaccgg ggcagggtgg ggtcagcgga gcaggtccag ggcgcgggcg gtgccgaggg
    10321 agtcgtcgac ggcggtgtgc cggggctcgg cctcgccacg cagcacctcg tggatgtggc
    10381 ggatgcccca ggagaacgtc gaaccctccc ccggctcgtg ccgctcgacc agccgctcct
    10441 cgcgccagac ctccagcacg tcgtgcggct tcgggtggaa gggattggtc aggcggatct
    10501 ccccgccggt gccgaccagc cgggcgtacg tcgtggacgg caggtcgaag ctgcagctca
    10561 gcagcaggcg gccgggatcg aattcgagca ctccggcggt ctcgatgtcc acccctgcct
    10621 cgcccttgac ggtggcggcg tgaccggcca cggcctcgcg gccgaacagc agttgcgcga
    10681 accgtacgga gtaacagccc acgtcgtaga gggcgccgcc gccgagggcc gcgtcgagcc
    10741 ggatgttctc cgcgccctca agccggaagt ggaaggacga ccagatctcc cgcaggtcgc
    10801 cgatgacgcc ctgttcgagg aggtcgcgca gccgggccgt ctgcgggtgg aacgggaaga
    10861 cgaacgcctc ccagagcggg cgggccgacc ggcgagccac gtcgatcacg ttctcggcct
    10921 ccgcggccgt caggcacagc ggcttctcgc acagcacggc cttgcccgcc tccagggcgg
    10981 cgatcgtcca ctgcgcgtgc agggcgttcg ggagcgggac gtagaccgcg tcgatgtccg
    11041 ggtcctcgat cacggcctcg tacccgcgca cggcacgctc gacaccgttc tgcccggcga
    11101 acgcctcggc ccgtgcgagg tcgcgacccg ccacgacggc ggccgt
//
