LOCUS       NZ_VANP01000001        10270 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Microbispora fusca strain NEAU-HEGS1-5 Scaffold1, whole genome
            shotgun sequence.
ACCESSION   NZ_VANP01000001
VERSION     NZ_VANP01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Microbispora fusca
  ORGANISM  Microbispora fusca
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Microbispora.
REFERENCE   1  (bases 1 to 1094452)
  AUTHORS   Yu,B.
  TITLE     Isolation, diversity and antifungal activity of Actinobacteria from
            wheat
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1094452)
  AUTHORS   Yu,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2019) Northeast Agricultural University, School of
            Life Science, Mucai59, Harbin, Heilongjiang 150030, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SOAPdenovo v. 2.04 OR JUL-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005864065.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:18:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,124
            CDSs (total)                      :: 7,053
            Genes (coding)                    :: 6,990
            CDSs (with protein)               :: 6,990
            Genes (RNA)                       :: 71
            rRNAs                             :: 4, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3, 4 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 11 of 63
            Pseudo Genes (incomplete)         :: 57 of 63
            Pseudo Genes (internal stop)      :: 6 of 63
            Pseudo Genes (multiple problems)  :: 11 of 63
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 19:39:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 261993
            Orig. end                         :: 272263
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            VANP01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10270
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10270
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10270
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5270](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5270)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    369..382
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.31"
                     /tool="antismash"
     misc_feature    411..424
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 22.67"
                     /tool="antismash"
     gene            complement(421..>525)
                     /locus_tag="FED44_RS37000"
                     /old_locus_tag="FED44_01280"
                     /pseudo=""
     CDS             complement(421..>525)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542673.1"
                     /locus_tag="FED44_RS37000"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01280"
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="ECYHQQYLFKVPNGYCGIGGTGVSCPVGLTAGDA"
     gene            complement(548..1066)
                     /locus_tag="FED44_RS01285"
                     /old_locus_tag="FED44_01285"
     CDS             complement(548..1066)
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]"
                     /GO_function="GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0009432 - SOS response [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /GO_process="GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542679.1"
                     /locus_tag="FED44_RS01285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01285"
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_111700553.1"
                     /transl_table=11
                     /translation="MSDVVFTAPAVDDLRRIGPDAAPKVLKKILLLLDNPEAGYPLGGE
                     LTGFRKLVVGRNTWRVVYRITDDKSIEICEVWAVGERADGEVYAEATARVRAAGGGRPE
                     VVQLGRVIERLGALAAHIRVEQPPSREPVPDWLADRLIYTVGMSREDVAALDLQQAVDL
                     WADFRSNPR"
     misc_feature    complement(1047..1062)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.81"
                     /tool="antismash"
     gene            complement(1070..1378)
                     /locus_tag="FED44_RS01290"
                     /old_locus_tag="FED44_01290"
     CDS             complement(1070..1378)
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542678.1"
                     /locus_tag="FED44_RS01290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01290"
                     /product="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
                     /protein_id="WP_138459482.1"
                     /transl_table=11
                     /translation="MTVLHEAAALSVTEAAQRGVARLVADAERGTDLVVTRRHQPVAAV
                     VSIRRLEELEEAAADLRDLALVLARSVTDTGERVPLDDVLSAFGHTRESLAALPDDE"
     misc_feature    complement(1392..1410)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 21.16"
                     /tool="antismash"
     misc_feature    1395..1413
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            complement(1647..2063)
                     /locus_tag="FED44_RS01300"
                     /old_locus_tag="FED44_01300"
     CDS             complement(1647..2063)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /locus_tag="FED44_RS01300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01300"
                     /product="PIN domain-containing protein"
                     /protein_id="WP_138459483.1"
                     /transl_table=11
                     /translation="MARARSKLSAGTVILDSEGLSKWCAADQRVTAIVREAQNNDFRVV
                     ISALTPIEAFDGRVTNDRLRWHLSRLRVEPVTEEVSVHALDLLRESGLHGHKYAIDAVV
                     AATALRSTRPVIVLTSDEDDMSKLCGKAVRLVRA"
     gene            complement(2039..2350)
                     /locus_tag="FED44_RS01305"
                     /old_locus_tag="FED44_01305"
     CDS             complement(2039..2350)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653588.1"
                     /locus_tag="FED44_RS01305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01305"
                     /product="hypothetical protein"
                     /protein_id="WP_138459484.1"
                     /transl_table=11
                     /translation="MSTPEQAFPPGEGPAAKVSVSLRAGNIRAIKERVGARGFSAYVDA
                     AVERQIERDLLEEALQANENECGPIPQHLRDEAEQLFRSVKSAPELQEERGWHAHEAS"
     gene            2549..2722
                     /locus_tag="FED44_RS35540"
     CDS             2549..2722
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FED44_RS35540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_167521354.1"
                     /transl_table=11
                     /translation="MAEIRRRWFLSEGEGGLPLSLKRSYIREALHAVIIYPSGKGRRRF
                     DPDLLELVWRED"
     misc_feature    complement(2958..2978)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.88"
                     /tool="antismash"
     gene            3405..4802
                     /locus_tag="FED44_RS01310"
                     /old_locus_tag="FED44_01310"
     CDS             3405..4802
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042633.2"
                     /locus_tag="FED44_RS01310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01310"
                     /product="replication initiator"
                     /protein_id="WP_138459485.1"
                     /transl_table=11
                     /translation="MSELPPFSPDVLAAAQRAAMPDFTRWQEMVRATGGCAQPIRLQGH
                     RITFDAGLEVLDVYRTADEPTGYLLTACGNRRASRCPACSEVYRDDTYHLILSGLRGGK
                     GVPEDVSAHPRVFATFTAPSFGAVHARRVKGDKVLPCRPRRDNPVCAHGRPEGCVVRHA
                     DNDHQVGQPICVDCYDYRGAVLWNAHAGELWRRFTQALPATFARHLGVSRAELRRRLRL
                     SYAKVAEYQARGLVHFHAVIRLDGPDGPSDRPPDGATVPVLQEAIRETAAVVSVPVPDD
                     DPSFVGRWGTQLDVDPIYVATELEGIADQRVAAYIAKYATKGAESAGTVDRPIREVADV
                     ARLDVTEHARRMIYTCFALGHDPAYRLIPLRQWAHMLGYRGHFSTKSRHYSTTLGALRQ
                     VRADHQAERARERRGLPARDERETLTVGQWRFAGSGYRNGEHLWAELIRQRIATARRIA
                     RERGKSA"
     gene            complement(5001..5270)
                     /locus_tag="FED44_RS01315"
                     /old_locus_tag="FED44_01315"
     CDS             complement(5001..5270)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /locus_tag="FED44_RS01315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01315"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_138459486.1"
                     /sec_met_domain="TIGR03651 (E-value: 9.2e-05, bitscore:
                     18.7, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEFRPDQLKWEAVLEEIRRRIRTGEYQVGHPIPGEPRMAEEFGI
                     AKGTARRVINELLKAGDIYTVLGKGTFVADPETGGPPRRDTEDE"
     gene            5402..5743
                     /locus_tag="FED44_RS01320"
                     /old_locus_tag="FED44_01320"
     CDS             5402..5743
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FED44_RS01320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FED44_01320"
                     /product="hypothetical protein"
                     /protein_id="WP_111700549.1"
                     /transl_table=11
                     /translation="MRQNMIRNRTADQQAGILAALGDLLRSQEVRTRLISHITLRMTGL
                     PVWDPAVSELDIYGLGGQVATVTIDQKHKPCRFLIKLPKSGTVVPLSTVDQPQSTVRWI
                     AGWANGRTR"
     misc_feature    6247..6260
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.84"
                     /tool="antismash"
     gene            6398..6904
                     /locus_tag="FED44_RS01325"
                     /old_locus_tag="FED44_01325"
     CDS             6398..6904
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FED44_RS01325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="FED44_01325"
                     /product="hypothetical protein"
                     /protein_id="WP_154956552.1"
                     /transl_table=11
                     /translation="MSFDVHVSYRLIYLADLADDMWAPETFPDDHPEHPVGIIRVKDGK
                     GLLVTGLHTGTVGFSVTVSDRDPEPDVNEYEDIVEISFHTQTGRIFLIEWGGEGFHKLP
                     DLSSGPGWYRLRYHAVHMDRATKSDCSTDAIIDRYLLQIWPHNESIPQVVKSTSHALTY
                     WRNAK"
     gene            complement(7182..7844)
                     /gene="msrA"
                     /locus_tag="FED44_RS01330"
                     /old_locus_tag="FED44_01330"
     CDS             complement(7182..7844)
                     /EC_number="1.8.4.11"
                     /GO_function="GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_function="GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542673.1"
                     /locus_tag="FED44_RS01330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01330"
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="WP_111700547.1"
                     /transl_table=11
                     /translation="MGWLFGRDKTTMVEPTDALPGRAEIMPVATRHQVLGAPLAPPYPE
                     GTEIAEFGLGCFWGAERKFWQTPGVVSTAVGYAGGYTPNPTYEEVCSGLTGHTEVVRVV
                     FDPAQVSYEELLKVFWEAHDPTQGMRQGNDVGTQYRSAVYTHGPEQLEAALASQEAYQK
                     VLTDAGYGEITTDIAPAGEFYFAEDYHQQYLFRNINGYCGIGGTGVACPIGLTTGEA"
     gene            7980..8885
                     /gene="rarD"
                     /locus_tag="FED44_RS01335"
                     /old_locus_tag="FED44_01335"
     CDS             7980..8885
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /gene="rarD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130561.1"
                     /locus_tag="FED44_RS01335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01335"
                     /product="EamA family transporter RarD"
                     /protein_id="WP_138459488.1"
                     /transl_table=11
                     /translation="MPDTRRGLLFGVAAYTMWGLFPLYWPLLKPSGAVEILAHRMVWSL
                     VVVVAVLAVRRHWSWIRTITRRQLILLTVAAVTVSVNWGTYIYAVNTGHVVESALGYFI
                     NPLVSVLFGVLVFRERLRVWQWAAVGLGAVAVLILTLDYGRLPWIALVLAFSFGTYGLV
                     KKKAGVGSAESLTIETLVLLVPALAYLLVLEATGTGTFAHHGAGHALLLVAAGLITAVP
                     LLFFTASALLVPLTVIGLLQYIAPVLQFLCGVLIVHETMPASRWAGFVVVWLALSVFTW
                     DSIRAARVSRRERAAELQTV"
     gene            8961..9788
                     /locus_tag="FED44_RS01340"
                     /old_locus_tag="FED44_01340"
     CDS             8961..9788
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /GO_process="GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544527.1"
                     /locus_tag="FED44_RS01340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="FED44_01340"
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="WP_138459489.1"
                     /transl_table=11
                     /translation="MSKDFRFGIVAGLAPDAAAWTATARRAENLGYSTLLVPDTLGTLP
                     PLLAMTAAATATTTLRVGTFVLAAPYRNPRLLAHELAGLDFLSGGRLEVGLGAGRPAAE
                     RDAHALGMPYGTAGERVAQVRSVIDEVRTAFAAGGLSVRPQGPPILVAGAGPRMLALAA
                     EAADIVALAVPAHTPEEGLRGPVGILRETAGARFDDIELSMNLFQVGDAPAPWLPEGSR
                     TIPDGAAAVLRGSPREMADTLLRRRDELGVSYVTVNGVYADAFAPVVEILTGR"
ORIGIN
        1 gcatccacgc cgagagcacg cggaacccga gcgatcggct ccagctgctc gccgcggaac
       61 tcgtcgacat ggcgccgccg cgtccacgcc aactgccgcc gcatcatctc ggcttcgcgg
      121 taccgctcca cctccgcatg agcgagccgg agaaccggga actgcgactc atcaccacac
      181 tgactcactt cggtaccgcc accgacgtca ccatcgcaga actgcgattg gaagcattcc
      241 taccggccga cgaaacgacc agcaccgcgc tgaccgagct cgttacgtgg tgaacgccat
      301 ctgctaaggc cgcgtatcgg atgttgccgg cggaatctcg cctgctcgca cggctctggc
      361 cctcgatcgg caccgggctt gccgggcgac cgagggccag gatcagtcag ttgccgcaga
      421 tcaagcgtca ccggcagtca ggccgaccgg gcagctcaca ccggtgccgc cgatgccgca
      481 gtagccgttc ggcaccttaa aaaggtattg ctggtggtag cactccaatt caggcagtaa
      541 ttcgcagcta gcgcgggttg gaccgaaagt cagcccagag gtcgaccgcc tgctgcaagt
      601 cgagagcggc gacgtcctcg cgagacatac cgaccgtgta gatcaggcga tccgcgagcc
      661 aatccggcac cggctccctg gagggcggtt gctcgacacg aatgtgagcg gccagcgcgc
      721 ccaagcgttc gatgacccgg ccaagctgca ccacctcggg acgcccccca ccggcagccc
      781 gaaccctggc tgtggcctcg gcgtagacct cgccatcggc cctctcgccc acagcccata
      841 cttcacagat ctcgatcgac ttgtcgtccg tgatccgata gacgacccgc caggtgttgc
      901 gcccgaccac gagcttgcga aagccggtca gctccccgcc cagcggataa cccgcctcgg
      961 gattgtcgag caaaagaagg atcttcttga gcaccttggg cgcggcgtcc ggaccgattc
     1021 gccgcagatc atcgaccgcc ggagccgtga agacgacgtc agacacgggc tactcgtcgt
     1081 ccggcagcgc ggccagcgac tcccgcgtgt gcccgaaagc ggagagcacg tcatcgagcg
     1141 ggacacgctc tccggtgtcg gtgaccgagc gtgcgagcac gagcgccaag tcccggagat
     1201 cggcggcggc ctcctccaac tcttccaggc gccgaatgct gacaaccgcc gccaccggct
     1261 gatgccgacg ggtcaccacc agatcggtcc cacgctcagc gtcagcgacg agacgcgcca
     1321 ccccacgctg agcggcttct gtgacactca gcgcggcagc ttcatgcagc acggtcatac
     1381 cactactgta caggagtctg tatagaaaat gcaggaggcg ctcagttccc gcaaggcgat
     1441 caccgtcggc agcgcggatc cgatctcatc gctgcccctc gtcaccttcc caaggggcaa
     1501 cgactcacag tcctgccagg tgactgatca ggcctcgccg gcggtcaggc cgacggggca
     1561 gctcacatcg atgccgcagt agccgttcgg caccttaaaa aggtattgct ggtggtagtc
     1621 gtatcgctgg ggcaataatt ctgcgcctac gcccgcacga ggcggactgc ctttccgcag
     1681 agcttgctca tgtcgtcctc gtccgaggtg aggacgatga cgggccgggt ggagcgaaga
     1741 gccgtggcgg ccacgacggc atcgatcgcg tacttgtggc cgtgaagacc gctctcacgg
     1801 agcagatcca gcgcgtggac ggagacctcc tcggtcaccg gctcgacacg cagccgggac
     1861 agatgccagc gcaaccggtc gtttgtcacc cggccgtcga aggcctcgat aggtgtcaag
     1921 gcactgataa caacgcggaa gtcgttgttc tgtgcctctc ggacgatcgc cgtgactcgc
     1981 tggtcggccg cgcaccactt cgagagtccc tcggagtcaa ggatgaccgt ccccgcactc
     2041 agcttgcttc gtgcgcgtgc catccacgct cctcctgtag ctccggggcg gacttcacgc
     2101 tacggaacag ctgctcggcc tcgtcccgca gatgctgcgg gatgggaccg cactcgttct
     2161 cgttcgcctg cagggcctcc tccagtaggt cccgctcgat ctgccgctcc acagcggcgt
     2221 cgacgtacgc ggagaacccg cgcgcgccga cacgctcctt gatggcacgg atgttgccgg
     2281 cccgcaagga gacgctcacc ttggctgcgg gcccttcccc cggcgggaag gcttgttcag
     2341 gcgtactcat ggcggcagtt tactactaca agtagcaaac caccggcacc ggacgcagga
     2401 aacctccgag tagcgccagg ccgctattag cagcgagctg ttcttctcgg cggctcccga
     2461 tctggagaag cgcatatccc gccttcgcac ggacgctcag agcttcgcgg cgacggctca
     2521 acgacgacag gcccgctccg ctaccgacgt ggccgagata cggcggcggt ggttcctgtc
     2581 ggagggtgag ggcggcctac cgctctcgct gaagcggtcc tacatccggg aggcgctgca
     2641 cgccgtgatc atctacccga gcggcaaggg gcgcaggcgc ttcgatcccg accttcttga
     2701 actggtctgg cgtgaggact gaggacacga gaacgcccaa gacgtccagg acgtctcgcg
     2761 gtcggcggta gctcctccag ccttaccgcc aggcgacaac ccccttacca gcctcccgcc
     2821 acagccggac gtctacggac gccgctggac gccagcggac accttgggac gccgtcaaag
     2881 acgtcatgcc aattccggct tcggaactga ccggtacgca tacgcgtcct actggggtag
     2941 aacagaactg taagaagtca ccgtctccct gacggaatga cacaggcgag aagctacgcg
     3001 acccattttc cggcgtgcat gagatccggg cggcaaacaa aacaactaac gccgtaccgc
     3061 tggagggacc cggcccagcg gcaggcgtca cgccctttca ctttctggtc gcacactccc
     3121 aggttctctc gctgacgtgc ccgtgttcgg ccgttcggct gctggctcac ggtgctcggc
     3181 gtctgttccg catgcgcggc ggtggctcca cccgcgagga aaggagccca cctgaccaca
     3241 cagaccctca tgtctgccca ggagtggggc agacgcaaag ccgccgagtc tcctcgttgg
     3301 tctgccgaca agtggcaccg ggtggccacc gttctcggtg ccgtgctggc cgacgaacct
     3361 ggccgtaccg agcccgccga cgagaagagc gggagggatg ccgcgtgagc gagcttcctc
     3421 ccttctcccc cgatgtcctc gctgctgccc agcgcgccgc catgccggac ttcacccggt
     3481 ggcaggagat ggtgcgcgcg acgggtggct gcgcccagcc gatccggctg caaggtcacc
     3541 ggatcacctt cgatgccggg ttggaagtgc tggacgtcta ccggacggcc gacgagccga
     3601 ccgggtacct gctgacggcg tgcggcaacc gtcgtgcgtc ccggtgcccg gcctgctcgg
     3661 aggtctaccg ggacgacacc taccacctga tcctgtccgg cctgcgcggc ggcaagggcg
     3721 tccccgagga cgtgagtgcc catccccgcg tgttcgctac gttcacggcc ccctcgttcg
     3781 gcgcggtcca cgctcgtcgg gtgaaggggg acaaggtgct gccgtgccgt ccccggcggg
     3841 acaacccggt gtgcgcgcac ggcaggccgg aaggctgcgt ggtccgccat gccgacaatg
     3901 accaccaggt cgggcagccg atctgcgtgg actgctacga ctaccggggc gcggtcctgt
     3961 ggaacgcgca cgccggtgag ctgtggcggc gcttcactca agctctgccc gcgacgttcg
     4021 cccggcacct cggcgtctcg cgggccgagc ttcggcgacg gctgcggctg tcgtacgcca
     4081 aggtggccga gtatcaggcg cggggcctgg tccacttcca cgcggtgatc cgtctcgacg
     4141 gtccggatgg tccgtccgat cgtccgccgg acggggcgac cgtgccggtg ctccaggagg
     4201 cgattcggga aaccgcggct gtggtctcgg tccctgtgcc ggatgatgac ccgtcgttcg
     4261 tcggccggtg gggcacgcag ctcgacgtgg accccatcta tgtcgcgacg gagttggagg
     4321 gtatcgcgga tcagcgggtg gcggcctaca tcgccaagta cgcgacgaag ggggccgagt
     4381 cggccgggac cgtggaccgg cccatccggg aggtcgccga cgttgcacgg ctcgatgtca
     4441 ccgagcacgc gcggcgcatg atctacacct gcttcgcgct cggccacgat ccggcctacc
     4501 ggctgatccc gctgcggcag tgggcgcaca tgctcggcta ccggggccac ttctcgacca
     4561 agagccgcca ctactcgacc actctcggcg cgctgcgtca ggtacgggcc gatcaccagg
     4621 ccgagcgggc gcgggaacgg cggggcctgc ctgctcgtga tgagcgggag acgttgacgg
     4681 tcggccagtg gcgcttcgcc gggagcggct accggaacgg cgaacacctg tgggccgaac
     4741 tgatccggca acgcatcgcc accgctcgcc ggatcgcccg tgaacgggga aagtcggcct
     4801 gaccttgcgg gatgtgcggc gcgagtggtg cggccggcgc aatgccgtag gcacccgaag
     4861 ggccgcgaag cggttgggcc ggcggcgcca cggcggaacg ctactgccct cggctcatcg
     4921 gctctacgct tggtgcgcct tccatcgtct cgctgacaac cgatagaagg ctgaaggccc
     4981 tcgaactcgt tcgggggcct tcactcgtct tcggtgtcgc gccggggcgg gccgccggtc
     5041 tcggggtcgg cgacgaaggt tcccttgccg agcacggtgt agatgtctcc tgctttgagc
     5101 agttcgttga tgacgcggcg tgcggtgccc ttggcgatgc cgaactcctc ggccatgcgg
     5161 ggttcaccag ggatggggtg acccacctgg tattcgcccg tccggatacg ccgtcgtatc
     5221 tcttcgagga cggcttccca tttgagctgg tcaggccgga actcgatcac gcgctcaaga
     5281 gtaggaacga aagcgttcca tgtcgttacc acaggtacct agaggtacct atgagaaggg
     5341 ctaggtagga ctaggaaggt cggcgtacgc tcaacccgtc agcgagacag atgggagagc
     5401 catgcgtcag aacatgatcc gcaaccggac cgccgaccag caggcgggca tcctggccgc
     5461 cctcggcgac ctactccgat cccaggaggt ccgtacccgg ctcatcagcc acatcaccct
     5521 gcggatgacc gggctccccg tctgggaccc ggcagtctcg gagctggaca tctacggcct
     5581 cggcggacaa gtcgcgacgg tcaccatcga ccagaagcac aagccctgcc gcttcctgat
     5641 caagctaccg aagtccggga ccgtcgttcc gctatccacg gtggaccaac cacagtccac
     5701 cgtccgctgg atcgccggct gggccaacgg caggacacgg taaggcggcg gtactgatgg
     5761 cggcctccat cccgcccctg gacaagtgcc tgagtccgca ctgggagatg cgccaaaaag
     5821 atcacgtgaa actcgtctgg catgagccct tcgagggggt ccggcggtcg cgagcactcg
     5881 cctggacctg cgactgccgg gctgtcgtct acgaactcct ccaggccggt gggcaggcgt
     5941 tcatccgcaa gaccacccag accgaaccca caccggggat cgaagagagc accgctggcc
     6001 catggaggag gcgcgcagag tgtggcatgc cctgctcctc ggcgagatgc gatagacagt
     6061 ccagccctcg cgcggccccg ctcacctccg gcggggccgc actcgcgttt acgacccgcg
     6121 ccactcctcc cccgcccccg atagctgccg acatcagtgg cccgaccgag gtgtcaatgc
     6181 cgtcttcgcg tgtccgtcca cccacgacgt acaccgcaac cccacaccca acccgcacag
     6241 acggcgttga caccgaccaa gggccacgaa caatcgagca gcgggggcgg aagaggttgg
     6301 aggtgcccaa ctagagaaac agccaggcgt cataagatgc gcggtattcc gaatcgttat
     6361 aaccgtagaa tccccgacca tgactaccaa tcggaccgtg tcgttcgatg tccacgtcag
     6421 ctaccgtctg atctacctcg ccgacctcgc cgacgacatg tgggctccgg aaaccttccc
     6481 cgatgaccac cccgagcacc ccgtgggcat cattcgggtg aaagacggta aaggtctcct
     6541 ggtcacgggc ctgcacacgg ggacggtcgg cttctcggtg accgtgtcgg accgcgatcc
     6601 agagccggac gtcaacgagt acgaggacat cgtggaaatc agcttccaca cccaaacagg
     6661 acggatcttc cttatcgagt ggggaggcga gggatttcac aagctacccg acttgtcgtc
     6721 aggcccgggc tggtaccggt tgcggtatca cgccgtccac atggaccggg ccacaaaatc
     6781 ggactgctcg acggatgcca tcatcgatcg ttatctgctg cagatctggc cacataatga
     6841 gtccatcccc caagtagtca agagcacgag tcacgcgctc acctactggc gcaatgcgaa
     6901 gtgacctccc aacgctccag ctcacttggg ctcgatcgcc aggatgaaaa aggttaactg
     6961 aatggctttt cagtacgctg cgcgcggccc gagtcgctgg cggccgggat cggcccccag
     7021 gccgcatgcc cggccgccga gcggacgcag agccgcgcgg cggcgcggag cgccgtcgcc
     7081 ttgaccctgt agtgaaggtt ctcgccgaag acggctctgg ccctcgatcc gcgccgggct
     7141 tgccgggcga ccaagggcca ggatcagtca gttaccgcag gtcaagcctc accggtggtc
     7201 aggccgatcg gacacgcaac gcccgtgcct ccgatcccgc agtacccgtt aatgttgcgg
     7261 aaaaggtact gctggtgata atcctcggcg aagtagaact ccccggcggg ggctatgtcg
     7321 gtggtgatct cgccgtaccc ggcgtcggtc agcaccttct ggtacgcctc ctgggacgcc
     7381 agggcggcct ccagttgctc cgggccgtgg gtgtagacgg ccgaccggta ctgcgtgccg
     7441 acgtcgttgc cctggcgcat gccctgggtg gggtcgtgcg cctcccagaa gaccttgaga
     7501 agctcctcgt acgacacctg ggccgggtcg aagaccaccc ggacgacctc ggtgtgcccg
     7561 gtgaggccgg agcagacctc ctcgtacgtg gggttgggcg tgtacccgcc cgcgtagccc
     7621 accgcggtcg agacgacgcc cggggtctgc cagaacttgc gctcggcacc ccagaagcag
     7681 cccagcccga actcggcgat ctccgttccc tcggggtacg gcggagccag cggcgcgccg
     7741 aggacctgat ggcgggtggc caccggcatg atctcggccc ggcccggcag cgcgtccgtg
     7801 ggctccacca tcgtcgtctt gtccctgccg aacagccagc ccatcgtgaa cctccccttc
     7861 tgttctgcca acaccaacca cccacccggc cgcgttgttc ctcccgtaag gatttctaaa
     7921 cgtcaggcgt agagctcctt atttgtcctg aatcgaggta agcagcgaag ataggcgaca
     7981 tgcctgatac ccgccgtggc ctcctgttcg gcgtcgccgc ctacacgatg tggggtctgt
     8041 tccctctcta ctggcccctg ctcaagccgt ccggcgcggt ggagatcctc gcgcaccgga
     8101 tggtctggtc gctcgtcgtg gtggtcgcgg tcctcgccgt acgacgtcac tggtcgtgga
     8161 tccgcaccat cacccgacgc cagctgatcc tcctcaccgt cgcggccgtc acggtctcgg
     8221 tgaactgggg cacctacatc tacgccgtca acaccggcca cgtggtcgaa agcgccctcg
     8281 gctacttcat caaccccctg gtcagcgtgc tcttcggggt gctggtcttc cgtgagcggc
     8341 tgcgcgtgtg gcagtgggcc gcggtcggcc tgggcgcggt cgcggttctc atcctgacgc
     8401 tcgactacgg gcgcctgccc tggatcgcgc tcgtgctcgc cttcagcttc ggcacgtacg
     8461 gcctggtcaa gaagaaggcg ggggtgggca gcgcggagag cctgaccatc gagaccctcg
     8521 tgctgctggt gccggccctg gcctacctgc tcgtcctgga ggcgaccggc acgggcacct
     8581 tcgcccacca cggcgccggc cacgccctgc tgctggtggc cgccggcctg atcaccgcgg
     8641 tgccgttgct gttcttcacc gcctcggcgc tgctggtccc cctcacggtc atcggcctcc
     8701 tgcagtacat cgcgccggtg ctgcagttcc tctgcggcgt gctgatcgtc cacgagacca
     8761 tgcccgccag ccggtgggcc ggcttcgtcg tcgtctggct cgcgctgagc gtcttcacct
     8821 gggacagcat ccgcgccgcc cgcgtctccc gccgcgagcg cgccgccgag ctccagaccg
     8881 tgtagtacgc ccccgggagt acggctcccg ccaccgacgg ggggacgacc tcgaccggcc
     8941 gcagcggcga ggatcggccc atgagcaagg acttccgctt cgggatcgtc gccggcctgg
     9001 cccccgacgc cgccgcgtgg acggccacgg cccgccgggc cgagaacctc ggctactcga
     9061 cccttctcgt ccccgacacg ctggggacgc tccctccgct gctcgccatg accgcggcgg
     9121 ccaccgccac gacgacgctg cgcgtgggca cgttcgtgct ggccgcgccg taccgcaatc
     9181 cccggctgct ggcacacgag ctggccgggc tcgacttcct gtccggcgga cggctggagg
     9241 tcggcctggg agccggccgc cccgccgccg agcgggacgc ccacgcgctc ggcatgccgt
     9301 acggcaccgc gggcgagcgc gtcgcgcagg tcaggtcggt gatcgacgag gtgcggacgg
     9361 cgttcgcggc gggcggcctc agcgtgcgcc ctcaggggcc gcccatcctg gtcgcgggcg
     9421 ccggtccccg catgctcgcg ctcgccgcgg aggcggccga catcgtcgcg ctcgcggtgc
     9481 ccgcccacac gcccgaggag ggcctgcgcg ggcccgtcgg catcctgcgg gagacggccg
     9541 gggcgaggtt cgacgacatc gagctgagca tgaacctctt ccaggtcggc gacgcgccgg
     9601 ccccgtggct gcccgagggg tcccgcacca tccccgacgg cgcggccgcc gtgctgcgcg
     9661 gttcgccccg ggagatggcc gacacgctgc tgcgccgccg tgacgagctg ggcgtctcct
     9721 acgtcaccgt caacggcgtc tacgccgacg cgttcgcccc cgtcgtcgag atcctcaccg
     9781 gccgctgaca cgcgcctctc ggcggcgtca gcgcgtggcc ccgatgcgcg accgggtgcg
     9841 tgcgggcgcc gactccggca gcgaggcgag gatgtcggcg agcgtgaccc ccgcgaggct
     9901 gccgcgccac gcggcgtgcg cctcgcccat cttctccgcg atcacacaga tgctccggca
     9961 gtcctcggga ggcagcgccc cgcgtcccct ctggcggatc tcacggcact catacggcga
    10021 agacgcgccg tcgacggcct cgacgatctg cagcaacgtg atgtccgagg ccgcccgccc
    10081 gagccggaag ccgccccgcg ggccggtcat cgcggccagc acaccggcct tcacgagcgc
    10141 ctgcagctgc ttggccaggt agggcgccgg gacgtcgtaa tactcagcca gctgtgccgc
    10201 cgacgcggtg gcgcccggct ccagctgcgc cagcgtggtg gcgcagtgca acacccactc
    10261 ggtgctcgcg
//
