LOCUS       NZ_CP048835            10423 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. So13.3 chromosome, complete genome.
ACCESSION   NZ_CP048835
VERSION     NZ_CP048835.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. So13.3
  ORGANISM  Streptomyces sp. So13.3
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Quezada-Solis,D., Khalil,Z.G., Capon,R.J.,
            Andreote,F.D. and Barrientos,L.
  TITLE     Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed
            Culture and Elicitation Conditions for the Production of
            Antimicrobial Compounds
  JOURNAL   Biomolecules 10 (5), 673 (2020)
   PUBMED   32349314
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Lamilla,C., Abanto,M., Maruyama,F., Jorquera,M.A.,
            Santos,A., Martinez-Urtaza,J. and Barrientos,L.
  TITLE     Antarctic Streptomyces fildesensis So13.3 strain as a promising
            source for antimicrobials discovery
  JOURNAL   Sci Rep 9 (1), 7488 (2019)
   PUBMED   31097761
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K. and Barrientos,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Centro de Excelencia en Investigacion
            Biotecnologica Aplicada al Medio Ambiente (CIBAMA), Universidad de
            La Frontera, Av. Francisco Salazar 01145, Temuco, La Araucania
            4811230, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2020
            Assembly Method                   :: Unicycler v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140.0x
            Sequencing Technology :: Illumina MiSeq; Illumina HiSeq; Oxford
            Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005860785.2-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:03:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,707
            CDSs (total)                      :: 8,616
            Genes (coding)                    :: 8,274
            CDSs (with protein)               :: 8,274
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 109 of 342
            Pseudo Genes (incomplete)         :: 268 of 342
            Pseudo Genes (internal stop)      :: 24 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:45:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8793469
            Orig. end                         :: 8803892
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Laboratorio de Biologia
            Molecular Aplicada, Universidad de La Frontera, Avenida Alemania
            0458, Temuco, Chile.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10423
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="28"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10423
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10423
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5423](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5423
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    629..642
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.21"
                     /tool="antismash"
     misc_feature    complement(1376..1391)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.82"
                     /tool="antismash"
     gene            complement(1460..2728)
                     /gene="efeB"
                     /locus_tag="C8250_RS39225"
                     /old_locus_tag="C8250_039265"
     CDS             complement(1460..2728)
                     /EC_number="4.98.1.1"
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /GO_process="GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="efeB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717657.1"
                     /locus_tag="C8250_RS39225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_039265"
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="WP_138356663.1"
                     /transl_table=11
                     /translation="MSQVRRRGFLRGAAAGIGTAVAGAAVSGAAVSDAGPSAATSAADS
                     TDTPTAPGDFHGAHQGGILTPPQRATAMVSFDVTAEGRRELTELLRTLTDRARFLAAGG
                     TPPPVGITAPPSDSGVLGPQVPSGGLTVTAGVGSSLFDGRFGLTDLKPSRLGVMPSFPD
                     DDLDPAWCHGDLSLQLSASSPDVVLHALRDIARHTRGGLQVRWRMDGFTSPPRPSGTPR
                     NHLGFKDGTANPDTAKATEADRLLWVGAGAEPRWTAGGTYQVVRLIRMLVEFWDRVSIT
                     EQERMFGRSRDTGAPLDGDAEFDAPRYTQDPKGDVIPLDSHIRLANPRTPQTEGQRILR
                     RGYNYDRGMDSNGNLDMGLIFSCYQQDLVRQFETVQKRLAGEPLVDYVQPFGGGYFFVL
                     PGVRDRTDWLGRALLTQNPHIRP"
     gene            complement(2725..3918)
                     /locus_tag="C8250_RS39230"
                     /old_locus_tag="C8250_039270"
     CDS             complement(2725..3918)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537664.1"
                     /locus_tag="C8250_RS39230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_039270"
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="WP_138356685.1"
                     /transl_table=11
                     /translation="MWLAGGVVASVALIAGVALWAGSPDGPQTADDPAPPHADDGLPHT
                     SVEVSRSSCGQGWVRPQPGTQVFDLRNTSSGAAEVDLLDPATGAVYGEVEGLAPGTTRP
                     MRVLLGNGTYAFKCLPDDADAVTGPTVRVSGGSAARGPAAVPVTAHDLIPPTLAYQQWI
                     QAGTVELAERTAALKDAVDAGDLAAARRAWLPAHLVYERMGAAYGTFGDADSAINGTTA
                     GLPGGLADPGFAGFHRVEYGLWHGESAASLRGPADALAEAVRALRDGWPQERMDPGDLG
                     LRAHEILENTVQRELTGRTDYGSGSNLATARANLDGTRELLGRLHPLLATRFPELPRLD
                     AWLDRTQHTLDSFDYDGTWAPLGTLPRADRQRINSDMSELVERLAPVAAMCDVRRTT"
     misc_feature    4166..4180
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 20.47"
                     /tool="antismash"
     gene            4209..4424
                     /locus_tag="C8250_RS39235"
                     /old_locus_tag="C8250_039275"
     CDS             4209..4424
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C8250_RS39235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="C8250_039275"
                     /product="hypothetical protein"
                     /protein_id="WP_232485944.1"
                     /transl_table=11
                     /translation="MSRDVEVLVDGKEVAHQRVRAGSSVLTAELPEDPAQPFQIRIHQP
                     RFGSSVPQCVLSLDDVDRTMPPRLVA"
     misc_feature    complement(4491..4505)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
     misc_feature    4756..4770
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     misc_feature    4871..4885
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.86"
                     /tool="antismash"
     gene            5001..5423
                     /locus_tag="C8250_RS39240"
                     /old_locus_tag="C8250_039280"
     CDS             5001..5423
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038019.1"
                     /locus_tag="C8250_RS39240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_039280"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_138356661.1"
                     /sec_met_domain="melC (E-value: 4.8e-45, bitscore: 152.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSELTRRSVLRGAVTGVAAAAFTGVALGLPVALAARGTGNDTGND
                     IDNTAGSVPHTGPEAFDETYRGRRIHGAPRAAGGHHHGGPGYAVFVDGKELHVMQNADS
                     SWISVVNHYETFATPRAAARAAVLELQGAALVPVAV"
     gene            5522..6340
                     /locus_tag="C8250_RS39245"
                     /old_locus_tag="C8250_039285"
     CDS             5522..6340
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091617.1"
                     /locus_tag="C8250_RS39245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_039285"
                     /product="tyrosinase family protein"
                     /protein_id="WP_138356660.1"
                     /transl_table=11
                     /translation="MAVRQNQANLTAAQKRAFTDAVLELKRSGRYDDFVTTHNAFIMSD
                     VDNGERVGHRSPSFLPWHRRFLLEFEQALQSVDSSVMLPYWDWTRDRTASASLWGADFL
                     GGDGRASDAQVTTGPFAFNSGRWAVNVRVDSRNYLRRQLGGGGRPLPTRAEVDSVLAMP
                     VYDMAPWNSQSNGFRNHLEGWRGVNLHNRVHVWVGGQMGTGVSPNDPVFWLHHCFIDKL
                     WADWQRLHPTSPYLPAAGTPDVVDLRDTMKPWNDITPADLLDHTSRYTYA"
     gene            6414..7205
                     /locus_tag="C8250_RS39250"
                     /old_locus_tag="C8250_039290"
     CDS             6414..7205
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006124107.1"
                     /locus_tag="C8250_RS39250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_039290"
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_138356659.1"
                     /transl_table=11
                     /translation="MSALSHDRHCSEIVAQTGLLYASIEGADLTVPVPSCPGWNAGQLL
                     RHLGGGQRWAEEVVRKRVLEPIPDDHFRDLSAYTHEDPAVLGPWLVEGAAQLAETLRGA
                     GPDTKVWTPVTGGTTAFYARRFLHETLIHRADATLAIGKKFTAEQEVALDGIDEWMELG
                     ALPMHFEVHPWMRELLGPGRTLHFHATDTPPQAAAEWLIDLTGDAIVWRRAHEKAAVAV
                     RGPLSDLLLIIYKRLPARTEGIEIIGDVELLDFWLERIGFG"
     gene            7514..7813
                     /locus_tag="C8250_RS39255"
                     /old_locus_tag="C8250_039295"
     CDS             7514..7813
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C8250_RS39255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="C8250_039295"
                     /product="hypothetical protein"
                     /protein_id="WP_138356658.1"
                     /transl_table=11
                     /translation="MEALAGGLAVQVAWGHVALATTESGLSHAGNSWSTALQVGWSAAV
                     AALLAVSGRRYAEFRRAGGNVRGLPALVGLALGLGLWRAGSWISTPASAAGHCR"
     gene            complement(8209..8988)
                     /locus_tag="C8250_RS39260"
                     /old_locus_tag="C8250_039300"
     CDS             complement(8209..8988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891154.1"
                     /locus_tag="C8250_RS39260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_039300"
                     /product="VOC family protein"
                     /protein_id="WP_138356657.1"
                     /transl_table=11
                     /translation="MLTTDFTTGAPNWLDLASPDTTATAGFYAAVFGWTFESAGPDAGG
                     YGLFQKDGKTVAALGQLTEEGAAPAWTVYFRTPDADATAKAVEQAGGTVRVEPFDVMDA
                     GRMSCLTDPGGAEFAVWQPGAVKGLEKTSEHNTLLWVELHVPDPSAALSFYRSLFGWRS
                     SEMKMPGMTYTVLSTAEGEQEDASFGGMVAAQGSMEPRWIPYFDAADADDIVTRVQENG
                     GTVLAPAEDVPDVGRIAWLADPFGAPFAVLKPRPRTA"
ORIGIN
        1 gacgacaacg gcggtgtcct tccactgcgg cgactgctgc accttgttga tctgctcgac
       61 gaggaagtgc tgctcatcga tcgggtcgga gtaggcggcg tggccgtcct ggaattccgg
      121 cgccttgagg aagctgaccg agggcaggtt ccccgccttc agcgcgctgt cgaagtcggt
      181 gaggtcgtag gtgtggttgg ccggcccgtt gtggccgatc tcggcgacgc tcgcgggcgc
      241 caggtggtgc gggttcgacg tcgacctgta gtactggaac ggcgagtggt gcgggctgta
      301 gtcaacgacc tgggcgccgc ccaggttggc gtgggtggtg ccggcgcact tcgcgtagtg
      361 gccctgctgc ccgtcccagg cggtactggg gcggaatccg ccctggaacc agccccagct
      421 gaccttctgg gcgttgagca gatcaccgat gttgcggccc tgcagaaccg cgagggcgtt
      481 cttgctgctg tggtcggcgt ccgagcagtc gtcgaaggcc gggtccgggt cgttgatcaa
      541 cgtgccgacg cccttggcgt cgggcgagac gacggcgtac gggtcgggcg tcgccgtctg
      601 cttcgggtgc tcggtgccgg aggccgggtc gacggagatc actccgtggg tctgcgcgga
      661 gatcagattg atggccccgg gggtcgacgg tccgtaggtc gtgccgtagg agttgtcgcc
      721 gagcgagtag tgctgggcgt agttccacat cgcggtgacg gtgttgccgt cgtagtagtc
      781 catgaccagg ccgggctcgc cgaacaggtt gccggagcac ttgccggagt cggtgttctg
      841 gacgtactcg tcggccttgc cgccgttggc cgcgtactgc tcggggccgt agttgtggtt
      901 ctggtcgcag gtcatggcct gctccgggcc gaggcgcttg ggcaggtact ggttcgggtt
      961 cttcttcagc aggccggcgg tgcgcagggt gtcgatgttc ttcggggtgc gcttgtccgc
     1021 ggtgaacgtc gtaccgtcgg tgttcgccgc caccgggtag gtgccgaagt agtggtcgaa
     1081 cgagacgttc tcctggaaga tgaccaccag atgcttgacc ggggtggtcg tcgcggcgcc
     1141 gccggggtgg tagttggggg agtccgcccg ggcggggccc gccccgctga ggagggtgag
     1201 tgccgcggca cctgcgcatg ctccccagcg cgcggcccgg cgtccgggat ccgtcgctct
     1261 tcctgcgttg cccatgttct gtcagcctcc gctgtcgacc ggtcgcgatg taggttacgg
     1321 aacgcgatcc ttcgttcggc ccgcgacacc ctgaccgcct atcagtgtcg tgcggatgaa
     1381 cagccggtca ggaacaacag gccaacgctt gacttgccgt tgaccgttcc gggcggggcg
     1441 tcaccgaagt cttcgactgc tatgggcgta tatgcgggtt ctgggtgagc agggcccgtc
     1501 ccagccagtc ggtgcggtcc cggactccgg gcaggacgaa gaagtagccg cccccgaacg
     1561 gctggacgta gtccaccaac ggctcgcccg cgaggcgctt ctgcacggtc tcgaactgcc
     1621 ggaccaggtc ctgctggtag caggagaaga tcagccccat gtcgaggttg ccgttgctgt
     1681 ccatgccccg gtcgtagttg tagccgcggc gcaggatgcg ctggccctcg gtctgcgggg
     1741 tgcgggggtt ggcgaggcgg atatgactgt cgagcgggat cacatcgcct ttgggatcct
     1801 gcgtgtacct gggcgcgtcg aactcggcgt caccgtccag cggcgcgccg gtgtcgcggc
     1861 tgcggccgaa catccgttcc tgctcggtga tcgagacccg gtcccagaac tcgacgagca
     1921 tccggatcag ccgcaccacc tggtaggtgc cgccggcggt ccaccgcggt tcggcgcccg
     1981 cgcccaccca gagcagccgg tccgcctcgg tcgccttcgc cgtgtccggg ttggcggtgc
     2041 cgtccttgaa cccgaggtgg ttgcggggcg tgccgctcgg gcggggcggg ctggtgaagc
     2101 cgtccatccg ccagcgcacc tgcagaccgc cgcgggtgtg gcgggcgatg tcgcgcagcg
     2161 cgtgcagcac gacgtccggg ctggacgcgg acagttgcag gctcagatcg ccgtggcacc
     2221 aggccgggtc cagatcgtcg tcggggaacg acggcatcac gcccagccgc gacggcttga
     2281 ggtccgtcag tccgaagcgg ccgtcgaaca gggagctccc caccccggcc gtgacggtca
     2341 gaccacctga cgggacctgc gggccgagca cgccggagtc ggacggcggt gcggtgatcc
     2401 cgaccggggg cggggtcccg ccggcggcca ggaaccgggc ccggtcggtg agcgtacgga
     2461 ggagctccgt cagctcccgg cggccctcgg cggtcacgtc gaacgagacc atcgccgtgg
     2521 cgcgctgcgg cggcgtgagg attccgccct gatgcgcacc gtgaaagtca ccgggcgcgg
     2581 tgggcgtgtc cgtggagtcg gcggcggacg tggccgcgct gggcccggcg tccgacacgg
     2641 cggctcccga caccgcggct ccggccaccg ccgtaccgat accggcggcc gcaccgcgca
     2701 ggaatccgcg tcgtcgcacc tggctcatgt cgttctccgt acatcgcaca tggccgccac
     2761 cggagccagc cgctccacca gttcactcat gtcgctgttg atccgctgcc ggtccgcgcg
     2821 gggcagcgtg cccagcggag cccaggtccc gtcgtagtcg aaggagtcga gggtgtgctg
     2881 ggtgcggtcc aaccaggcgt ccagtcgcgg cagttcaggg aaccgggtcg ccagcagcgg
     2941 gtgcagccgg ccgagcagct cacgtgtccc gtcgagattg gcgcgggcgg tggcgaggtt
     3001 gctgccgctg ccgtagtcgg tgcgtccggt cagttcccgc tgcacggtgt tctccaggat
     3061 ctcgtgcgcg cgcagcccca gatcccccgg atccatccgc tcctgcggcc acccgtcccg
     3121 cagcgcccgc accgcctcgg cgagagcgtc agcgggcccg cgcagcgacg ccgcggactc
     3181 cccgtgccac agcccgtact cgacgcggtg gaaccccgcg aagccggggt cggcaaggcc
     3241 gccgggcagc cctgccgtcg ttccgttgat cgcggagtcc gcgtccccga acgttccgta
     3301 cgccgcgccc atccgctcgt agaccagatg cgcgggcagc cacgcgcgcc gggccgccgc
     3361 caggtcgccg gcgtcgacgg cgtccttcag cgcagcggtc cgctccgcca gctcgacggt
     3421 tccggcctgg atccactgct ggtaggccaa cgtcggcggg atgagatcgt gcgccgtcac
     3481 gggcacggcg gccggtcccc gcgcggccga ccctccgctc acccgtaccg tgggaccggt
     3541 gacggcgtcc gcgtcgtcgg gcaagcactt gaacgcgtac gtaccgttcc ccagcagcac
     3601 gcgcatcggc cgggtcgtgc cgggcgccaa cccctccacc tcgccgtaga ccgcgccggt
     3661 ggccggatcc aggaggtcga cctcggcggc cccgctggag gtgttgcgga gatcgaacac
     3721 ctgcgtcccc ggctgggggc gtacccagcc ctgtccgcag ctgctccgcg acacctccac
     3781 ggaggtgtgc ggcagaccat cgtccgcgtg cggcggcgcg gggtcgtccg ccgtctgcgg
     3841 gccgtccggc gaccccgccc acagggcgac ccccgcgatc agtgccacgg aggcgaccac
     3901 gcctcccgcc agccacacct gccggcggcg tcggtgccac ggcggccggc caggatctcc
     3961 gacctgcccc gcaggatcgt cggtgtcctg gccggggtcg gagacggggc cggggccggg
     4021 cacagcgggc ctccaaggat gtcggcggtg ggaaggcggc gtcgttgcat ggtagggcga
     4081 agcgcgggag tcgaaggacg ggtttacgct ggtagtgagg tccgctccgg aggtgcgctg
     4141 tgccccgacg acatcatttc cacctggatc atctcggcca ttcgatcacc gtcaatatcc
     4201 gctccggcgt gagccgtgac gtcgaggtgt tggtcgacgg caaggaagtc gcccaccagc
     4261 gtgtccgcgc cgggtcgagt gtgctgacgg ccgaactgcc ggaagacccc gcccagccgt
     4321 tccagatccg catccaccag ccacgcttcg gctccagcgt gccccagtgc gtcctgagcc
     4381 tggacgacgt ggaccggacg atgccgcccc gcctcgtggc gtgagcctcc gttacttcac
     4441 cctgtgcagg ccggcaacga cgggacggcg cgctgcactc cgtggttctg atccacgagg
     4501 gtgatccgtc gcaggtgtca tggttgaagt ccggtcaacg gccgggtccc ggggcgtgat
     4561 ggtgcgccct cagggcccgg ccgcgctgtt tgatccagcc cgtgatccac ctcgtgatcc
     4621 acctccgcgt ccgccgccgc ggcgctcctg gcggccggtc cgctgctcac cgtcgaacac
     4681 ttttgcgtcg tgatcttttg gtgggggaca tcaccataat tcccgtacaa cccgaggatt
     4741 tgtcgctcct tcggcttcat tcaggagatt ttcgcgtctg aacccgtata gcgccggacg
     4801 ttctgcaaga tccactccgt tcaactttcc acatcttgcg agggaatgtc cggtaaggag
     4861 ccaacgggta agacactcct ttccactcgc ggccgccgcc tgcgaacatc ccggtcggga
     4921 gatcacgaaa tgtgactacc cgttgggatt cctgccttct ggggtcgatg tacccgaagg
     4981 cgacaagatg ggggccgtac atgtctgaac tcacccgccg cagcgtgctg cgcggcgccg
     5041 tcacgggcgt tgccgccgcc gcgttcaccg gggtcgcgct gggcctgccg gtggcgctgg
     5101 ccgcccgggg caccgggaac gacaccggga acgacatcga caacaccgcg gggtccgtcc
     5161 cgcacaccgg acctgaggcg ttcgacgaga cctaccgggg ccggcgcata cacggcgcgc
     5221 cgcgggccgc cggcggacac caccacgggg ggccgggcta cgcggtgttc gtggacggca
     5281 aggaactgca tgtgatgcag aacgccgact cctcctggat cagcgtcgtg aaccactacg
     5341 agacgttcgc cacgccgcgt gctgcggccc gggccgcggt gctcgaactg cagggcgccg
     5401 cgctcgtccc ggtcgctgtc tgacccgatc gtcgcccgac ccgaaccgcc gcaccttaat
     5461 ccgacccgtc gcgacgcgac ccgtcggaac ccgaaccacc cgcaaaggac gaagactctt
     5521 catggcagtc cgtcagaacc aagcgaacct caccgccgca cagaagcgtg ccttcaccga
     5581 cgccgtactg gaactcaagc gcagtggccg gtacgacgat ttcgtcacca cgcacaacgc
     5641 gttcatcatg tccgacgtcg acaacgggga gcgggtcggc caccgatcgc cgtccttcct
     5701 gccctggcac cgcagattcc ttctcgagtt cgagcaggcg ctgcagtccg tcgactcctc
     5761 ggtcatgctg ccgtactggg actggacccg ggaccgtacg gcgtccgcct ccctgtgggg
     5821 cgcggacttc ctcggcggcg acgggcgcgc cagcgacgcg caggtgacca ccggcccgtt
     5881 cgccttcaac tccggacggt gggcggtgaa cgtccgcgtc gactcccgca actatctgcg
     5941 ccgccagctc ggcggcggag gccgccccct gccgacccgg gccgaggtcg attccgtcct
     6001 cgccatgccc gtgtacgaca tggcgccgtg gaacagccag tcgaacggct tccgcaacca
     6061 cctggagggc tggcgcggag tcaatctgca caaccgggtc catgtgtggg tcggcggcca
     6121 gatgggcacc ggcgtgtcgc ccaacgaccc ggtgttctgg ctgcaccact gcttcataga
     6181 caagctgtgg gcggactggc agcgactgca cccgacctcg ccgtacctgc cggccgcggg
     6241 gacgccggac gtcgtcgacc tgcgcgacac catgaagccg tggaacgaca tcaccccggc
     6301 cgacctgctc gaccacacct cccgctacac gtacgcgtga cctgacccgc ccggcggcgg
     6361 aggcgcggac caggagcggt ggcgctgtcg gaggtgcggt ctatcgtgac gtcatgagcg
     6421 ctttgagcca tgaccgccat tgctccgaga tcgtcgcgca gaccggcttg ttgtacgcct
     6481 ccatcgaggg tgcggacctc acggtcccgg tgccctcctg ccccgggtgg aacgccggcc
     6541 agctgctgcg gcacctcggc ggcggccagc gctgggccga ggaagtggtc cggaagcggg
     6601 tcctggagcc gatcccggac gatcacttcc gtgatctgtc cgcgtacacc cacgaggatc
     6661 ccgcggtgct cggaccgtgg ctcgtcgagg gcgccgcgca gctggcggaa acgctgcgcg
     6721 gcgccgggcc ggacacgaag gtgtggaccc cggtcaccgg gggcaccacc gcgttctacg
     6781 cgcgccggtt cctgcacgag acgctcatcc accgggccga cgcgaccctg gcgatcggca
     6841 agaagttcac cgccgagcag gaggtcgccc tcgacggcat cgacgagtgg atggagctcg
     6901 gcgctctgcc gatgcacttc gaggtccacc cctggatgcg cgaactcctc ggccccggcc
     6961 gcaccctgca cttccacgcc acggacaccc cgccgcaggc ggcggcggag tggctgatcg
     7021 atctcaccgg cgacgccatc gtctggcgcc gggcccatga gaaagccgca gtcgccgtac
     7081 gcggcccgct gtccgacctc ctgctgatca tctacaaacg cctcccggcc cggaccgaag
     7141 gcatcgagat catcggcgac gtggaactgc tcgacttctg gctggaacgc atcggcttcg
     7201 ggtgacgcgc cctcccggat ttcgacgcgg tatcggcccg tcgaccgcga cccggccgag
     7261 cccgtcccgg cgctgtccgg cgggttctcg ccgaagggcg taggttccgg caacttcctc
     7321 cccccgctgg atctccaggc cgacccggcg ctcgccgggg aactgcgcac cctggtcgcc
     7381 cgtgccacgc cggcccgtcc gccgacgtgg ctgacggatc cggtggtggc gtgccgcgtg
     7441 ggccaagcat ccctcccggc cggcgcggac ggcggccctg gcccaacctc gtgtcccgct
     7501 gcgccccgga gtcgtggaag ccctggcggg cgggttggcc gtgcaggtgg cgtggggtca
     7561 tgtggcactg gccaccaccg agtcgggcct gtcccatgcg ggcaacagct ggtccaccgc
     7621 actgcaggtc gggtggagtg cggcggtggc ggcgctcctg gcggtctcgg gccggcggta
     7681 cgccgagttc cggcgagccg gcgggaacgt ccgcggcctg ccggcacttg tcgggctcgc
     7741 tctcggcctg ggactgtggc gggccggttc ctggatctcg acaccggcgt cagcagccgg
     7801 tcactgccgt tgacctggat cggagcgatc gacgcgctgt tgctgctcgg cttctcggtg
     7861 gggctcgcca cctgggtggc ctacctggcc ggcgccgggc tcgggatccc ggtcggagca
     7921 ggtcgcagtc aggtcccgga gggcccgaac ggcaggccgg gcggtgccct ttgtcgtcgc
     7981 ggtcgcgtgt gtcgctcatg gcggccacgc cggcggcttc gcgggacgtg ctcaaggtcg
     8041 gcgaggtgtc ccggaccacg cccgtgggac ggggcggcgg aactgcacat ctgggtcgtc
     8101 ggaggcgggg accggcggaa gcagctgctg acgatgatga tcgattcgct gacccactga
     8161 tgcggctgcg gcccggggtc cccggtccgc accaccctcc cgatggggtc aggcagttcg
     8221 ggggcggggc ttgaggacgg cgaagggcgc gccgaacggg tcggcgagcc aggcgatccg
     8281 gccgacgtcc ggtacgtcct cggcgggtgc gaggacggtg ccgccgttct cctgcacccg
     8341 ggtgacgatg tcgtccgcgt cagccgcgtc gaagtacgga atccagcgcg gctccatgct
     8401 cccctgggcg gcgaccattc cgccgaacga agcgtcctcc tgctcgccct cggcggtgga
     8461 gagcacggtg taggtcattc cgggcatctt catctcgctc gaccgccagc cgaagagcga
     8521 acggtagaag gacagcgcgg ccgacgggtc aggaacgtgc agctcgaccc agagcagcgt
     8581 gttgtgttcc gacgtcttct ccaggccttt gaccgccccc ggctgccaga cggcgaactc
     8641 ggcgccgccc gggtcggtga ggcaggacat ccgtcccgcg tccatgacgt cgaagggctc
     8701 gacccgcacg gtcccaccgg cctgctcgac cgccttggcg gtggcgtccg cgtccggggt
     8761 ccggaagtac accgtccagg ccggggcggc gccctcctcg gtgagctgcc ccagggccgc
     8821 gaccgtcttg ccgtccttct ggaacaagcc gtagccgccc gcgtcggggc cggcggactc
     8881 gaaggtccag ccgaagaccg ccgcgtagaa gccggccgtc gccgtggtgt cggggctggc
     8941 gaggtcgagc cagttgggtg cgccggtggt gaagtcggtc gtgagcatcg tgacgatcct
     9001 gcctctcgcg aatgcggtcg gagcatgagc gcggggagat ccaccccgag gtgaacgatc
     9061 cctagcggat cgtagcgaaa cgcccaccgg aggacatcgc gaatggcgga gcggcggcct
     9121 cgaaaacggg ggccggcggc tcctgtgggc ctggagccgc cggcccgccg gccgcgcggt
     9181 gcggggtccg cggtgcgggg tccgggtcct gggcgggtca gatgccgtcg gtgaacagca
     9241 gcttgttggg ggaggtcttg ctgaccgagc tcaggacgcc cttggtggcg gtgtccgcca
     9301 accaggccgt gatgtcctgc ggagtcgcgg ccggatgggt ggtcttgtac agcagcgcgg
     9361 tcccggcgac atgcggagag gccatggagg tgccgtccag agcgacgctg ccgccgccga
     9421 gcttggccga cacgatggcc tgtccggggg cgtagagcga cacgcacttg ccgtagttgg
     9481 agaagtcggt ctcctggtcc tgccggttgc tcgcgccgac ggccacgacg ctccccgccg
     9541 acgccgggga ggtggtgcag gcgtcggtcg cttcgttgcc cgccgcgacg acgggcagga
     9601 cgcccaggtc gaggagggag ctgacggcgc tgttgaccat ggaggacacc gggccgccga
     9661 gtgaggcgtt gaggaccgcc ggcttgccgg ggttcatcgc cgcgttcgct cccgcccatt
     9721 cgagtccggc gatgactttc agactgctgc tcttgccggc acagtcgagg acccggacgc
     9781 tgatcagcgt cgcctggcgt gcgacgccgt acgtcttgcc gccgatggtg cccgcgacgt
     9841 gggtgccgtg gccctcgcag tccgctccgt tgcgtccctg ggacgggaac gcgtcgaagc
     9901 cgggaacggc gcggccctcg aactcactgt ggttgtaatc gatgccggag tcgaggacat
     9961 aggcgttggc gccggcaccc gtggcggtca cggtgaaggt gttgtccagc ggcaggttcg
    10021 gctggtcgat ccggtccagg ccccaggaca ccgaaggcgc caccgcgccg gcggccgcgg
    10081 tcgtcttcgc cggggtgccg ggctccggcg cgcggaactc ggcgtcctgc tccacggcca
    10141 ggacgttcgg gttcgccctg accttggcca gctgagcggc cgacatcttg gcgccgaagc
    10201 cccggagcac cctgctgaac ctgaacaggg aggacacccc ggacttggtc accgcatcgg
    10261 cgacgttggc cttcggcttg agctggacga tgtactggtc ggcgatgggg gacgacgccg
    10321 ggacgcggag cggcgcgaga acgggtgcgc ccgccccggc ggggccgggc ttcgcggcgg
    10381 cggacagcgg acctgcggtc accagggcag cggtgacggc ggt
//
