LOCUS       NZ_CP048835            11908 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. So13.3 chromosome, complete genome.
ACCESSION   NZ_CP048835
VERSION     NZ_CP048835.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. So13.3
  ORGANISM  Streptomyces sp. So13.3
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Quezada-Solis,D., Khalil,Z.G., Capon,R.J.,
            Andreote,F.D. and Barrientos,L.
  TITLE     Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed
            Culture and Elicitation Conditions for the Production of
            Antimicrobial Compounds
  JOURNAL   Biomolecules 10 (5), 673 (2020)
   PUBMED   32349314
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Lamilla,C., Abanto,M., Maruyama,F., Jorquera,M.A.,
            Santos,A., Martinez-Urtaza,J. and Barrientos,L.
  TITLE     Antarctic Streptomyces fildesensis So13.3 strain as a promising
            source for antimicrobials discovery
  JOURNAL   Sci Rep 9 (1), 7488 (2019)
   PUBMED   31097761
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K. and Barrientos,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Centro de Excelencia en Investigacion
            Biotecnologica Aplicada al Medio Ambiente (CIBAMA), Universidad de
            La Frontera, Av. Francisco Salazar 01145, Temuco, La Araucania
            4811230, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2020
            Assembly Method                   :: Unicycler v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140.0x
            Sequencing Technology :: Illumina MiSeq; Illumina HiSeq; Oxford
            Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005860785.2-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:03:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,707
            CDSs (total)                      :: 8,616
            Genes (coding)                    :: 8,274
            CDSs (with protein)               :: 8,274
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 109 of 342
            Pseudo Genes (incomplete)         :: 268 of 342
            Pseudo Genes (internal stop)      :: 24 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:45:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8508334
            Orig. end                         :: 8520242
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Laboratorio de Biologia
            Molecular Aplicada, Universidad de La Frontera, Avenida Alemania
            0458, Temuco, Chile.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11908
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="27"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11908
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11908
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6908](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6908
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(202..1044)
                     /locus_tag="C8250_RS37985"
                     /old_locus_tag="C8250_038015"
     CDS             complement(202..1044)
                     /GO_function="GO:0015267 - channel activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551375.1"
                     /locus_tag="C8250_RS37985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_038015"
                     /product="MIP/aquaporin family protein"
                     /protein_id="WP_138355640.1"
                     /transl_table=11
                     /translation="MADTTDAPKVVGRLASHRLLGELLAEFLGTLVLILFGVGVVAQVV
                     AADLGDHDSIAWAWGFGVTFGVYVAARLSGAHLNPTVTLALAIFRGFPWARVAPYALAQ
                     TAGAFTAALLVRWNSSEVLAKADPGHTIKTQGVFSTLPGNGVFPISEWGALRDQIIGTA
                     ILLLVIMALTDVLNTAPKANLGPLLIGFLVVAIGMAIGTDAGYAINPARDFGPRLASYI
                     TGYNSAWRDQYGDLYFWVPIVGPLIGGPIGAAVYHYVVRPFLPEHAEQPAGSILPEDE"
     misc_feature    complement(1396..1409)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.17"
                     /tool="antismash"
     misc_feature    complement(1404..1418)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.98"
                     /tool="antismash"
     gene            1520..2851
                     /locus_tag="C8250_RS37990"
                     /old_locus_tag="C8250_038020"
     CDS             1520..2851
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019357535.1"
                     /locus_tag="C8250_RS37990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_038020"
                     /product="MFS transporter"
                     /protein_id="WP_138355641.1"
                     /transl_table=11
                     /translation="MTAARRTSPGSQSRDFWLYWSAGTSDALGTQASGLVLPLLLLGLG
                     SSPGTVGVLASVSAIGGLLAGPPASVLADRRWRRGMMVGSALVAAGAMGAIAAAAAWGR
                     PSLLLLCSAALVERVATACYEAAARGTLVTVVAPVHYPRAIARLEAGEQGALVAGPALG
                     GLLFPLARWLPFLMDALSYVFSALCVRAIRADLNPHQKSEDSPPRFTEEFLAGLRFVRN
                     ESFLRFVLLWTAGVNGLLTALYYGAVFSIRGQGSGTASTGLVLALSGAAGLVGAAAAPR
                     LARRIPAARIVIAASWTMVPVAAGLAFATHAWVYGLLLGSVSLTVPAVAVVLHSRAVLV
                     TPDQLQSRVGTTLDTACGVASVLAPAAAGLLTDHLGSTRTALLCAAGLAVLAAYATATA
                     ARRFLPSDHDPDREPGSDNDPATDPNHDPALLSRPQPLEAGEPS"
     misc_feature    2168..2170
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2848..4761
                     /locus_tag="C8250_RS37995"
                     /old_locus_tag="C8250_038025"
     CDS             2848..4761
                     /NRPS_PKS="Domain: Abhydrolase_1 (373-491). E-value:
                     1.8e-08. Score: 26.2. Matches aSDomain:
                     nrpspksdomains_C8250_RS37995_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569707.1"
                     /locus_tag="C8250_RS37995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_038025"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_138355642.1"
                     /sec_met_domain="PF00561 (E-value: 7.1e-05, bitscore: 21.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 1e-34, bitscore:
                     118.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDLPAVPATPVGRDSGLGYQVYRPVLPATCRRSPGLMVLTGDVD
                     GRCEVFAWDAATGAARQVTDRPYGTLLYAIDADAVIWWFDEDRNGRGTWWIQPFSGGPD
                     TPGLTGTGPGRPCGLAVSDSGTVALGLGDEHGLRVHLGPRGGAGREVLRTDGHGLLVDL
                     APAGDLLAVARGAAGKDAVTLLTPDGAVVESLPGTDGRLWALGFAPGRSTPELRPGRST
                     PELLLVAERDGRYVIATWRADTGLELHRWCSFDTEITARWCPQGRTVLVRQDRHGRSRL
                     VTADLDRRELAPVAVPVGTVLDAAPQPGGDVHYLWTDALTPPRTRSLLGAPLPGLEAVP
                     PPIAGRADDVWTQGPDGPVHTLLTVPSGTAGPYPLVFLVHGGPAAHDRDAYDPAVHSLV
                     ESGFAVARVNYRGSTGYGPRWRAAYGQGVGLTQVADLALVRADLVRRGIARADAIGLWG
                     TSWGGYLVLLALGTRPDLWQAAVAVKPLADCATAFAAGTPALRALDTELFGGTPLQVPE
                     IYARSSPSSYAASVRAPLLVVAATQDAKCPPEQVETYLAALRASGVRHDALWLDSGHDG
                     HDGGDHVQVLHRALVFLDRHLRGPRRPQRAAYEELPAPSGVGARGPAAEPAGSTRRNAE
                     RR"
     aSDomain        3967..4320
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C8250_RS37995_Abhydrolase_1.1"
                     /evalue="1.80E-08"
                     /label="C8250_RS37995_Abhydrolase_1.1"
                     /locus_tag="C8250_RS37995"
                     /protein_end="491"
                     /protein_start="373"
                     /score="26.2"
                     /tool="antismash"
                     /translation="PLVFLVHGGPAAHDRDAYDPAVHSLVESGFAVARVNYRGSTGYGP
                     RWRAAYGQGVGLTQVADLALVRADLVRRGIARADAIGLWGTSWGGYLVLLALGTRPDLW
                     QAAVAVKPLADCAT"
     gene            5001..6908
                     /locus_tag="C8250_RS38000"
                     /old_locus_tag="C8250_038030"
     CDS             5001..6908
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359084.1"
                     /locus_tag="C8250_RS38000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_038030"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_138355643.1"
                     /sec_met_domain="TIGR03975 (E-value: 2.1e-180, bitscore:
                     600.0, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILKRSVDEKTPGASAEVLHANLEY
                     VDWITRRREFTLEDYEYFSLSSYFMGCGDWVFSAALYEDPQWRVAEFTEAMKGRLRKER
                     MAMTLELHEDAAEFVQLMADRIVAAEPDVVGFTSTFQQNTAALAAAKYVKRMAPHIRIV
                     MGGANCDGAQGESVHRNFPFVDFVIRGEGEQTFPALLTALQEDTDLTKVPGLCHRVDGR
                     SVANPMSTVPMPPAAILPPDYTGYFERLVASKARSWVEPKLVVEGARGCWWGEKHHCTF
                     CGLNGSSMQFRSKSPDTFYEEIIGLAEKHKVLDMYVVDNILDMGYLSTVLPCIIESDYD
                     LRMHIEIKSNMRVNQLQILADAGLIYVQPGIESLNSRVLDLMDKGVSGTQNVRMLRDGA
                     AVGLSISWNYLHGFPGETAADYDDVIAQIPALEHLNPPVDLSARIAIERFSPYFNKPEL
                     GFSELRPAEPYRLTYDLPESEMLDLAYVFDVPPRGVGDETVKPLNDALAAWKTNYRSSR
                     LTHADLGDRVILVNRRRAFDWTTLELADPVQVSAFRLLEQPHAPAALTRKIAARNPGVA
                     VTEASVEELLLEWRRLGVVFTDGGQYVHVAPPAVNEDMLRLDFMRYAHQVRQVQPPALV
                     "
     gene            7087..7662
                     /locus_tag="C8250_RS38005"
                     /old_locus_tag="C8250_038035"
     CDS             7087..7662
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958122.1"
                     /locus_tag="C8250_RS38005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_038035"
                     /product="DUF5825 family protein"
                     /protein_id="WP_232485138.1"
                     /transl_table=11
                     /translation="MLLGRLEVQGGGSAAVRQLWDQGARRVELPGVVDLTDPTAAADTV
                     RTLCLIRDLTARAVYVDWRLRTGPDPEAWLPLNHLHPPLEVEGPPDPEEVVRQWRDGHY
                     LCKCIWRNGPGFVQIRDRRWGELHRFTAEEPHYHRAITLLEHGAPAAELPADVLADFRE
                     ERLIGEVGELAWWLPYRIQRWIHTAMVV"
     gene            7821..8957
                     /locus_tag="C8250_RS38010"
                     /old_locus_tag="C8250_038040"
     CDS             7821..8957
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018487794.1"
                     /locus_tag="C8250_RS38010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_038040"
                     /product="substrate-binding domain-containing protein"
                     /protein_id="WP_138355644.1"
                     /transl_table=11
                     /translation="MRMHVDQRHELVLKLVRERGSIRVADLAAELGVSSVTLRRDVETL
                     AEQGRVRRLHGSVVWPHDPQAADRAAPSAPAAPAGGGQGPVIGMVVPTTDYYFAAVVAG
                     AREAAAARGARLVIGLSQYLPHEDRAQAARLLSTGADGLLLTPSWGHGVPEPGQEEWIA
                     QQGVPAVLVERWAPLGSPAAALDRVRTDHAHGAALAVRHLASLGHRTVALAAQESPTAV
                     QLAIGHAAAVEALGLDPAPRSPLELEPSRDESDRFDRTLEYLCDAVGKHGVTATLVHSD
                     ADAIVLIPRLQARGVRVPEDLALIAYDDEVAGLADLPLTAVAPPKRVVGALAADLLLDR
                     LAEHSQGAPAQPRRHVELLPELRVRQSCGAAAGSTLSE"
     misc_feature    9011..9024
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            9087..10415
                     /locus_tag="C8250_RS38015"
                     /old_locus_tag="C8250_038045"
     CDS             9087..10415
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1143: sugar-binding lipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606385.1"
                     /locus_tag="C8250_RS38015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_038045"
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="WP_138355645.1"
                     /transl_table=11
                     /translation="MKPISQPTSRTTRLALAAVVASLALATTACGGDKAAAGGAAPDAK
                     PVTLTYWGWAKGAQATVDAFNKTHKDIHVRYSEIAGGPDGYSKITNAVKAGNAPDVAGI
                     EYTQLPEFASQGLLEDLTERAGDTVKQKFPASIQDLVTFGGKTWAVPYDVAPQLYYYRT
                     DLFQKYGIAVPKTWAEFKTAGEKLQQADKNVHIASWPDNDAVLLAALAWQGGGKWFSTE
                     GDAWKVGINDAASQKVASYWQGLVDSGVVNSIPAGSEDETKARTSGTVASFLGASWSAG
                     GMTVAMPKLSGKWGIAPLPTWDGKPASGGYGGTTYAVPKGSKHVKEAAEFSRWVTTDAA
                     AVTARLSSLTSPSSALPANVEMRDVAAKAFKGGAYYAGFDVYKIAGAQVDTIVPGWTFG
                     PVQIATNTAITDAAAKGNLTQGLTAGQASAEKGIKERGLNLVK"
     gene            10536..11462
                     /locus_tag="C8250_RS38020"
                     /old_locus_tag="C8250_038050"
     CDS             10536..11462
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010062460.1"
                     /locus_tag="C8250_RS38020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_038050"
                     /product="sugar ABC transporter permease"
                     /protein_id="WP_244809634.1"
                     /transl_table=11
                     /translation="MAVPLARTPRRRAAARPGQRGAVTVLLVPFFALFGLVMVAPIGYA
                     VWMSLFQERSSGLGFGGTERVFSGLGNYSRALSDPAFRTSFLHIAVYCVAYIPVMIGGA
                     LVLALLLDSALSRAKRFLQLALFLPHAVPSMIAAIIWIYLYTPGLSPVTGWIESLGGNW
                     AFFSADHALSSMINIAVWQWIGYNMVIFYAALQAVPRETLEAAVVDGAGELRIAWQIKV
                     PVIRSSVVLTLLFTCVGAVQLFNEPKILSSRASSLGSTWSPTMYIFKAAFERHDYGLAA
                     ASSLLLALAAAALSFIVSRIGNRGKTA"
ORIGIN
        1 cagctgatgg cggccgacct cgttaccgcc atgatcgaag atcatgaaac gggtgctcgt
       61 cgtgccctgg tcgatcgcgg cgacgtagtc cgccatgtcg gttcctccac gcgtgatcgc
      121 tgctgacggc cggccgggac ggccggtgga acggctccgt gccggggacg ggatccgggt
      181 atctgtcgga gacggccgcg gtcactcgtc ctcgggcagg atgctgcccg ccggctgttc
      241 ggcgtgctcc ggcaggaacg gtctgacgac gtagtggtag acggcggcgc cgatcgggcc
      301 gccgatgagc gggccgacga tgggcaccca gaagtagaga tcgccgtact ggtcgcgcca
      361 cgcgctgttg tagccggtga tgtagctcgc cagccgcgga ccgaagtcac gggccgggtt
      421 gatcgcgtac ccggcgtcgg tgccgatggc catgccgatc gccaccacga ggaacccgat
      481 cagcaggggg cccaggttcg ccttgggagc ggtgttgagt acatcggtca gcgccatgat
      541 gacgagcagc aggatggccg tgccgatgat ctggtcgcgg agcgcgcccc attcgctgat
      601 cgggaagacg ccgttgccgg gcagggtgga gaacaccccc tgggtcttga tggtgtgacc
      661 cgggtccgcc ttggcgagga cttcggagga gttccagcgc accagcagcg cggcggtgaa
      721 ggctcccgcc gtctgtgcca gcgcgtacgg ggcgaccctc gcccatggga agccgcggaa
      781 gatggccagc gcgagggtca cggtcgggtt gagatgcgcg cccgacagcc gtgccgcgac
      841 gtacacaccg aaggtgacgc cgaaccccca cgcccacgcg atgctgtcgt ggtcgcccag
      901 gtcggcggcg acgacctggg cgaccactcc cacgccgaac aggatgagca ccagggtgcc
      961 gaggaactcg gcgagcagtt cgcccagcag ccggtgcgag gccagccggc ccaccacttt
     1021 gggagcatct gtcgtatcgg ccatcggtcc agcccttccc gacgcgtggg cccgctcccc
     1081 gtcgcggtgc cggtactgcg atgtgccggc gtcacgacgt gtcggtggac ccgcttccga
     1141 ctcacgtctg acccattcga caggtacagc ggcccggcgg caccgcgggg agcccctcgt
     1201 gctgccgaac gagtgacgcg gcgcttcccg ccgccccgcg gcggccaaag gccgaggtca
     1261 tggaggggcg gcggggcgcg gccgttccac cggggaagag aagtccgcag aaagcccgag
     1321 aggacttgag tcgtgctcga ataccggtcg aatcgtggcc aattctttct caatggcacg
     1381 tcaactcgaa gtaatggatc gtatgttcat attgcggagc cggaatccgc ctcgctacgc
     1441 ttccggggat tcgagaggaa acactcccct tttccccatc tctgcgggta ttccgcccgg
     1501 gacgtaggac ggcctcgcca tgaccgctgc acgtcgcacg tcgcccggca gccagtcccg
     1561 ggacttctgg ctgtactggt cggcgggcac ctccgacgcg ctcggcaccc aggcctcggg
     1621 cctggtactc ccgctgctgc tgctcggtct cggctcgtcg cccggcaccg tcggcgtcct
     1681 ggcctcggtc tccgcgatcg gtggactgct ggccgggccg cccgcgtccg tgctggccga
     1741 ccggcgctgg cgacggggga tgatggtggg atccgcgctg gtggcggcag gtgcgatggg
     1801 tgcgatagcc gctgccgcgg cctggggccg gccctcgctg ctccttctgt gctcggccgc
     1861 gctcgtcgag cgggtggcca cggcgtgcta cgaggcggcc gcccgcggga cgctggtcac
     1921 cgtggtggcg ccggtccact atccgcgtgc catcgcgcgg ctggaggcgg gggagcaggg
     1981 cgcgctggtg gccggccccg cgctgggcgg cctgctgttc ccgctggccc gctggctgcc
     2041 gtttctcatg gacgctcttt cctatgtgtt ctccgctctc tgcgtacggg ccatccgagc
     2101 cgatctcaat ccgcaccaga aatcggagga ttcgcctccg cgctttaccg aagaattcct
     2161 ggccggatta cgtttcgtgc gcaacgaatc gtttcttcga tttgtgctgc tgtggacggc
     2221 aggcgtcaac gggctcttga cagcgctgta ttacggcgcg gtgttcagca tccgcggcca
     2281 gggctccggc accgcgtcca ccggtctcgt cctcgcgctg tcgggggccg ccgggctggt
     2341 cggcgccgcc gccgcgcccc ggctcgcacg gcggatcccc gccgcgcgga tcgtgatcgc
     2401 cgcctcctgg accatggtgc cggtcgccgc cggcctggcc ttcgccaccc acgcctgggt
     2461 ctacggcctg ctgttgggct ccgtgtcgct gaccgtcccg gcggtcgcgg tcgtcctgca
     2521 ctcgcgggcc gtcctggtga cccccgacca gctccagtcc cgggtcggca ccacgctgga
     2581 caccgcctgc ggagtcgcct ccgtcctggc cccggccgcc gccggactgc tcaccgacca
     2641 cctcgggtcc acccgtacgg cactgctctg cgcggccggg ctggctgtgc tcgccgccta
     2701 cgccaccgcg accgcggccc gccggttcct gccctcggac cacgaccccg accgtgaacc
     2761 cggctccgac aacgacccgg ccaccgaccc gaaccacgac cccgccctgc tgtcccgtcc
     2821 ccagccgctg gaagccggtg agccctcatg agcgatctgc ccgccgtccc cgccaccccc
     2881 gtcggccggg actccggact gggctaccag gtctaccggc ccgtgctgcc cgccacctgc
     2941 cggcggtcgc ccggtctgat ggtgctcacc ggggacgtcg acggccgctg cgaggtcttc
     3001 gcgtgggacg ccgccaccgg cgccgcccgc caggtcaccg accgcccgta cggcaccctg
     3061 ctgtacgcca tcgacgccga tgccgtcatc tggtggttcg acgaggaccg gaacggccgc
     3121 ggcacctggt ggatccagcc cttctccggc ggtcccgaca ccccggggct gaccggcacc
     3181 ggacctggcc gcccctgcgg gctggccgtc agcgacagcg ggaccgtcgc gctcgggctc
     3241 ggggacgagc acggactgcg cgtccacctg ggcccccggg gcggcgccgg acgcgaagtg
     3301 ctgcgcaccg acggtcacgg cctgctggtc gatctcgccc cggcgggcga cctgctggcc
     3361 gtcgcgcgcg gtgcggccgg aaaggacgcg gtcaccctgc tgacaccgga cggcgctgtg
     3421 gtggagagcc tgccgggcac cgacggtcgg ctgtgggcac ttggcttcgc ccccgggcgc
     3481 agcacccccg aactgcgccc cgggcgcagc acccccgaac tgctgctcgt cgccgagcgc
     3541 gacggccgct acgtgatcgc cacctggcgc gccgacaccg gactcgaact gcaccgctgg
     3601 tgctccttcg acaccgagat caccgcccgc tggtgcccgc agggccgcac ggtactggtc
     3661 cgccaggacc ggcacggccg cagcaggctg gtcaccgcgg acctcgaccg gcgtgaactg
     3721 gcgcccgtcg ccgtccccgt gggcaccgtg ctcgacgcgg ccccgcagcc cggcggcgat
     3781 gtgcactacc tgtggaccga cgcgctcacc ccgccgcgca cccgctccct gctcggtgcg
     3841 ccgctgcccg gtctcgaggc ggtaccgccg ccgatcgccg gccgcgccga cgatgtgtgg
     3901 acccagggtc ccgacggccc cgtgcacacc ctgctcaccg tgcccagcgg cacggcggga
     3961 ccgtacccgc tggtcttcct ggtgcacggc ggccccgccg cgcacgaccg ggacgcctac
     4021 gacccggccg tgcactcgct cgtggagtcc ggcttcgccg tcgcccgcgt caactaccgc
     4081 ggctccaccg gctacggacc gcgctggcgg gccgcgtacg gacagggcgt gggactgacc
     4141 caggtcgccg atctcgcgct ggtccgcgcc gacctggtgc gccgcggcat cgcccgcgcg
     4201 gacgcgatcg gcctgtgggg gacctcctgg ggcggctacc tggtgctgct ggcgctgggc
     4261 acccgtcccg acctgtggca ggcggccgtc gcggtcaaac cgctggccga ctgcgcgacg
     4321 gcgttcgcgg ccggcacccc ggcgctgcgc gccctggaca ccgagctgtt cggcggcacc
     4381 ccgctgcagg tgcccgagat ctacgcgcgt tcgtccccgt cgagttacgc cgcctcggtc
     4441 cgggcaccgc tgctggtggt cgccgcgacc caggacgcca agtgcccgcc ggaacaggtc
     4501 gagacgtatc tggcggcgct gcgcgcctcc ggcgtccggc acgacgcgct gtggctggac
     4561 agcggtcatg acgggcacga cggcggcgac cacgtacagg tgctgcaccg ggcgctggtc
     4621 ttcctggacc ggcatctgcg cggcccccgt cgtccgcagc gcgccgcgta cgaagagctc
     4681 cccgccccgt caggggtggg ggccagggga ccggcggccg aacccgccgg ttccacccgc
     4741 cggaacgcag agaggaggta gccaccatgc agaaggacgt catccacaac gatccgctcg
     4801 aggacgaggc ggagaaccgg aagccgggcg tgggggtggt catcaggatc cccttccgta
     4861 acgccgacga gccggagaac agctgagtcc ggaggccggc ccggctcaag ccgccgggcc
     4921 ggcccactcc gcaccacccg atgcaccccg ttaccgaatc cctgtgctga acccctgtgc
     4981 gacgaggaag agaagaagcc gtgcgcgtac tcctggtgaa catgccctgg tcccccatcg
     5041 accttccgtc gctcgcgctg ggcatcctca agcgcagcgt cgacgagaag acccccggag
     5101 cgagtgcgga ggtgttgcac gccaacctcg aatacgtgga ctggatcacc cgacggcggg
     5161 agttcacgct ggaggactac gagtacttct cgctctcctc ctacttcatg ggatgcggcg
     5221 actgggtctt ctccgccgcc ctgtacgagg acccgcagtg gcgggtcgcc gagttcaccg
     5281 aggcgatgaa gggccggctg cgcaaggagc gcatggccat gaccctcgaa ctgcacgagg
     5341 acgcggccga gttcgtccag ctgatggccg accggatcgt ggcggccgag cccgacgtcg
     5401 tcggcttcac ctcgacgttc cagcagaaca ccgcggcgct ggccgcggcc aagtacgtca
     5461 agcgcatggc cccgcacatc cgcatcgtca tgggcggcgc caactgcgac ggcgcgcagg
     5521 gcgagtcggt gcaccggaac ttcccgttcg tcgacttcgt gatccgcggc gagggcgagc
     5581 agacgttccc cgcgctcctg accgcgctcc aggaggacac cgacctgacg aaggtccccg
     5641 gtctgtgcca ccgggtggac gggcggagcg tggccaaccc gatgagcacc gtgccgatgc
     5701 cgccggccgc catcctgccg cccgactaca ccggctactt cgagcggctg gtcgcgtcga
     5761 aggcccggtc ctgggtggag cccaagctgg tggtcgaggg cgcccgcggc tgctggtggg
     5821 gcgagaagca ccactgcacg ttctgcggcc tcaacggctc gtcgatgcag ttccgcagca
     5881 agagcccgga caccttctac gaggagatca tcggcctcgc agagaagcac aaggtgctgg
     5941 acatgtacgt cgtcgacaac atcctcgaca tgggctacct cagcaccgtc ctgccgtgca
     6001 tcatcgagag cgactacgac ctgcgcatgc acatcgagat caagtccaac atgcgcgtca
     6061 accagctgca gatcctcgcc gacgccggac tgatctatgt gcagcccggc atcgagagcc
     6121 tcaacagccg ggtgctcgac ctgatggaca agggggtcag cggcacccag aacgtgcgga
     6181 tgctgcgcga cggcgccgcg gtggggctgt ccatctcctg gaactacctg cacggcttcc
     6241 ccggcgagac cgccgccgac tacgacgacg tcatcgccca gattccggca ctcgagcacc
     6301 tcaacccgcc ggtcgacctg tcggcgcgga tcgccatcga acggttcagc ccctacttca
     6361 acaagcccga actgggcttc tccgaactgc gtcccgcgga gccctaccgg ctgacgtacg
     6421 acctgccgga atcggagatg ctcgatctgg cgtacgtctt cgacgtgccg ccgcgcggcg
     6481 tgggcgacga gacggtcaag ccgctcaacg acgcccttgc cgcctggaag acgaactacc
     6541 gcagcagccg tctcacccac gcggacctcg gtgaccgcgt catcctggtc aaccgccggc
     6601 gcgccttcga ctggaccacc ctcgaactcg ccgaccccgt ccaggtgtcc gcgttccggc
     6661 tgctggagca gccgcacgcg ccggccgccc tcacccgcaa gatcgccgcc cgtaacccgg
     6721 gggtggcggt caccgaggcg tcggtcgagg agctgctgct cgaatggcgc cggctgggag
     6781 tggtgttcac cgacggcggc cagtacgtcc acgtggcgcc gccggcggtc aacgaggaca
     6841 tgctgcggct ggacttcatg cgctacgccc accaggtccg gcaggtacag cccccggccc
     6901 tggtgtgacc ggccccggca ccttctcgtc gctggatcag caccggcccc gcacacaaca
     6961 gcaccagcgt ccgcgcacgc gaaggagact ccgttgagca tcaccaccga caccacccgg
     7021 ccaccggcca cgctgacgat caccgcctgg cgcgaccacg atccggccgc ctgcgagctt
     7081 cccggcatgc tgctgggacg gctggaggtc cagggcggcg gatcggcggc ggtccggcaa
     7141 ctgtgggacc agggcgcgcg ccgggtcgaa ctgccgggcg tcgtcgatct gaccgacccc
     7201 accgcggccg cggacaccgt ccgtactctg tgcctcatcc gcgatctgac cgcccgcgcc
     7261 gtctacgtcg actggcggct gcgcaccgga ccggatccgg aggcctggct gccgctgaac
     7321 catctgcacc cgcccctgga ggtggagggt ccgccggacc ccgaggaggt ggtgcgccaa
     7381 tggcgcgacg ggcactacct gtgcaagtgc atctggcgca acggtcccgg cttcgtgcag
     7441 atccgcgacc ggcgctgggg cgaactgcac cgcttcaccg ccgaggagcc gcactaccac
     7501 cgggcgatca ccctgctgga gcacggcgca ccggccgcgg agcttcccgc ggacgtgctg
     7561 gccgacttcc gcgaggagcg gctgatcggt gaggtcggcg agctggcgtg gtggctgccg
     7621 taccggatcc agcgctggat ccacacagcg atggtggtgt gagctccgct ctccttcagc
     7681 tggtgtcgtg gcggcctcct gcgcaggcga tcgatcgtaa cgatcggaaa gcgatcgata
     7741 tgatagaggc gtagagtgcg gtcatgtcgt gagcggatcg cacacggctg gccattgctg
     7801 ccgcgaccag ggggaatcca atgcggatgc acgtcgacca gcgtcatgaa ctggtgctga
     7861 agctcgtccg cgaacggggc agtatccggg tcgccgacct ggccgccgag ctgggcgtct
     7921 cctccgtcac gctacgccgg gacgtggaga cgctggccga gcagggtcgg gtgcgcaggc
     7981 tgcacggatc ggtcgtctgg ccgcacgatc cgcaggccgc cgaccgggcc gcgccgagcg
     8041 ctcccgccgc cccggccggc ggcggccagg ggcccgtgat cggcatggtc gtgcccacca
     8101 ccgactacta cttcgcggcc gtcgtcgccg gcgcccgcga ggccgccgcc gcccgtggcg
     8161 cgcgcctggt catcggcctg tcgcagtacc tgccgcacga ggaccgcgcg caggccgcgc
     8221 ggctgctgtc gacaggcgcc gacgggctgc tgctcacccc gagctggggg cacggcgtgc
     8281 cggagcccgg ccaggaggag tggatcgcgc agcagggcgt tcccgccgtg ctggtggagc
     8341 gctgggcgcc gctcggcagt ccggcggcgg cgctggaccg agtgcgtacc gaccacgcgc
     8401 acggcgccgc gctcgccgtg cgccatctgg cctcgctcgg gcaccgtacg gtcgcgctgg
     8461 ccgcccagga gagcccgacc gccgtgcagt tggccatcgg gcacgcggcc gcggtcgaag
     8521 cgctgggact cgacccggcc ccccggtcgc cgctggaact cgagccgtcg cgcgacgagt
     8581 ccgaccggtt cgaccggacg ttggagtacc tgtgcgacgc ggtcggcaag cacggggtga
     8641 cggcgacgct ggtgcacagc gacgcggacg ccatcgtgct catcccacgg ttgcaggcgc
     8701 gcggtgtgcg ggtgcccgag gacctggccc tgatcgcgta cgacgacgag gtcgcgggtc
     8761 tcgcggatct gccgctgacg gcggtcgccc cgccgaagcg cgtggtcggg gcgctcgccg
     8821 ccgacctgct gctcgaccgg ctcgcggagc actcccaggg ggccccggcg cagccgcgcc
     8881 ggcatgtgga gctgctgccg gagctgcggg tacggcagtc ctgcggtgcg gcggccggta
     8941 gcacgctgag cgagtgatcg cagttatctg atcgatattc gatctagacg atcgaatcac
     9001 tcattcgctc ttgacggcga tcgatgttgc gcaaatgatg gcctgcattc acctcatctg
     9061 tcggggtcac cgtaggagca cgcgcaatga agcccatctc ccagcccacc tcccgtacga
     9121 cccgtctcgc gctcgcggcc gttgtggcct ccctcgcgct ggccaccacc gcctgcggtg
     9181 gggacaaggc cgcggccggc ggcgccgcgc cggacgccaa gccggtcacc ctgacgtact
     9241 gggggtgggc caagggggcc caggcgaccg tcgacgcgtt caacaagacc cacaaggaca
     9301 tccacgtcag gtactcggag atcgccgggg gtccggacgg atactccaag atcaccaacg
     9361 cggtcaaggc cggcaacgcc cccgacgtgg cggggatcga gtacacccag cttccggagt
     9421 tcgccagcca gggactgctc gaggacctga ccgagcgggc cggcgacacc gtcaagcaga
     9481 agttcccggc ctccatccag gacctggtga ccttcggcgg caagacctgg gccgtgccct
     9541 acgacgtcgc gccccagctg tactactacc gcacggacct gttccagaag tacggcatag
     9601 ccgtccccaa gacctgggca gagttcaaga ccgccggcga gaagctccag caggccgaca
     9661 agaacgtgca catcgcgtcc tggcccgaca acgacgccgt gctgctcgcg gccctcgcct
     9721 ggcagggcgg cggcaagtgg ttcagcaccg agggcgacgc ctggaaggtc ggcatcaacg
     9781 acgccgcgtc ccagaaggtc gcctcctact ggcagggact cgtcgacagc ggcgtcgtca
     9841 actccatccc ggccggcagc gaggacgaga ccaaggccag gacctccggc accgtcgcca
     9901 gcttcctcgg tgcctcctgg agcgcgggcg gcatgaccgt cgcgatgccg aagctgagcg
     9961 gcaagtgggg gatcgccccg ctgcccacct gggacgggaa gcccgccagc ggcggctacg
    10021 gcggcaccac gtacgccgtg ccgaagggca gcaagcacgt caaggaagcg gccgagttct
    10081 cccggtgggt caccacggac gccgccgccg tcaccgcccg cctcagctcg ctgacctcgc
    10141 cgagcagcgc cctgcccgcc aacgtcgaga tgcgtgacgt ggccgcgaag gcgttcaagg
    10201 gcggggccta ctacgcgggc ttcgacgtct acaagatcgc cggtgcgcag gtcgacacca
    10261 tcgtcccggg ctggacgttc ggccccgtgc agatcgcgac caacaccgcc atcaccgacg
    10321 cggcggccaa gggaaacctg actcagggac tgacggcagg tcaggcgtcc gccgagaagg
    10381 gcatcaagga gcgcgggctg aacctggtca agtgaccgcc gggccggcgg gaccgcccgc
    10441 cggccccgtc gtccggtctc cggccacccg ccgggcacgc gctgacgtgc ccggcgggcc
    10501 ccactcccca cgaccccgct cccaaggagt cccccgtggc agtaccactc gcccggaccc
    10561 cgcgccgccg cgccgccgcc cgaccgggcc aacgcggcgc cgtgaccgtg ctcctggtcc
    10621 cgttcttcgc cctgttcggc ctcgtgatgg tggcgcccat cggctacgcg gtgtggatga
    10681 gcctgttcca ggaacgctcc tccggtctcg gcttcggcgg caccgaacgc gtcttctcgg
    10741 gcctgggcaa ctactccaga gccctgtccg acccggcgtt ccgtacctcg ttcctgcaca
    10801 tcgccgtgta ctgcgtcgcc tacatcccgg tgatgatcgg cggcgcgctc gtcctcgcgc
    10861 tactcctgga ctccgccctc agcagggcga agcgcttcct gcagctcgcc ctcttcctgc
    10921 cgcacgcggt gcccagcatg atcgcggcga tcatctggat ctacctctac acgccgggcc
    10981 tcagcccggt caccggctgg atcgagagcc tcggcggcaa ctgggccttc ttctccgccg
    11041 accacgcgct ctcctcgatg atcaatatcg cggtgtggca gtggatcggc tacaacatgg
    11101 tcatcttcta cgccgcgctg caggccgtcc cgcgcgagac gctggaggcg gcggtggtcg
    11161 acggggcggg cgaactgcgg atcgcctggc agatcaaggt gccggtgatc cgctcgtccg
    11221 tggtgctgac actgctgttc acctgcgtcg gcgcggtcca gctcttcaac gagccgaaga
    11281 tcctctccag tcgggcctcc tccctcggct cgacctggtc accgaccatg tacatcttca
    11341 aagccgcctt cgaacgccat gactacggtc tggccgccgc gtcctcgctg ctgctggccc
    11401 tggccgccgc cgcactctcc ttcatcgtca gcaggatcgg aaaccggggg aagacggcat
    11461 gagcaccctt gatctcgacg gccgccaggc cgtccccggc acgcttccgg ccacccccgt
    11521 tcgcgaagga cccgggccgg accaccgccc caccggacgc tcccggcgcc ggcgccccac
    11581 cggcgccctg ctgtcccggg cggcagtcaa cggcctgctc gccgtcgccg ccctctacac
    11641 cctgatgccg ctggcctggc tgctgatcgc ggcgaccaag gaccacggcg atctgttcgg
    11701 caccggcggc ttcaccctcg gcgcgttcca cctcttcgac aacctccggg ccgtgttcac
    11761 cgccgacggc ggcatctacg gccgctggct gctcaacagc ctgctgtact cgctcgtggg
    11821 ctcgctcgtc tcgaccctca tctgcgtggc cgccggatac gccttcgaca agtaccactt
    11881 cgcgggcaag gacaagctct tcggtgtc
//
