LOCUS       NZ_CP048835            22182 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. So13.3 chromosome, complete genome.
ACCESSION   NZ_CP048835
VERSION     NZ_CP048835.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. So13.3
  ORGANISM  Streptomyces sp. So13.3
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Quezada-Solis,D., Khalil,Z.G., Capon,R.J.,
            Andreote,F.D. and Barrientos,L.
  TITLE     Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed
            Culture and Elicitation Conditions for the Production of
            Antimicrobial Compounds
  JOURNAL   Biomolecules 10 (5), 673 (2020)
   PUBMED   32349314
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Lamilla,C., Abanto,M., Maruyama,F., Jorquera,M.A.,
            Santos,A., Martinez-Urtaza,J. and Barrientos,L.
  TITLE     Antarctic Streptomyces fildesensis So13.3 strain as a promising
            source for antimicrobials discovery
  JOURNAL   Sci Rep 9 (1), 7488 (2019)
   PUBMED   31097761
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K. and Barrientos,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Centro de Excelencia en Investigacion
            Biotecnologica Aplicada al Medio Ambiente (CIBAMA), Universidad de
            La Frontera, Av. Francisco Salazar 01145, Temuco, La Araucania
            4811230, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2020
            Assembly Method                   :: Unicycler v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140.0x
            Sequencing Technology :: Illumina MiSeq; Illumina HiSeq; Oxford
            Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005860785.2-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:03:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,707
            CDSs (total)                      :: 8,616
            Genes (coding)                    :: 8,274
            CDSs (with protein)               :: 8,274
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 109 of 342
            Pseudo Genes (incomplete)         :: 268 of 342
            Pseudo Genes (internal stop)      :: 24 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:45:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7239396
            Orig. end                         :: 7261578
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Laboratorio de Biologia
            Molecular Aplicada, Universidad de La Frontera, Avenida Alemania
            0458, Temuco, Chile.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22182
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="19"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22182
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22182
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12182](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12182
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     gene            complement(62..991)
                     /locus_tag="C8250_RS32355"
                     /old_locus_tag="C8250_032365"
     CDS             complement(62..991)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491528.1"
                     /locus_tag="C8250_RS32355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032365"
                     /product="sugar ABC transporter permease"
                     /protein_id="WP_244809883.1"
                     /transl_table=11
                     /translation="MDPPPALRRRRRPVLGAVWPYLLIAPTLVGTAFLLLYPLIRNTVM
                     SFQHFRLGELIRGGATFVGFDNYSEVLNDPEFWTVVRRTLWWTALNVVLIMLIGTLVAL
                     MLARLGKRMRLAVMSALVLTWATPVIAATTIFQWLFQSRFGVVNWVLVELGFDSYRDYS
                     WFADGNSTFFILVTLVVWQSVPFAALTLYGGLTTISAELYESARLDGAGGFQVFRHVTF
                     PMLRQLFALITSLEVIWCFKCFAQIWVISEGGPNGATTTLPVYAFQIAQSLHKYDVGSA
                     VSTLTVLILLVALVLNLRRMFQQEGAAL"
     gene            complement(1123..2397)
                     /locus_tag="C8250_RS32360"
                     /old_locus_tag="C8250_032370"
     CDS             complement(1123..2397)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1143: sugar-binding lipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019982388.1"
                     /locus_tag="C8250_RS32360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032370"
                     /product="extracellular solute-binding protein"
                     /protein_id="WP_138358076.1"
                     /transl_table=11
                     /translation="MRTRLLAGTAALAIAVAGLSACSSSSDSSDTAGDGKSPAELTVWI
                     MKGSVSDPFLQRFKTDFETTHSGTTLKIQIQEWDGIGAKITSALASKDAPDVIEVGNTQ
                     VAQYAASGGVKDLTGKVADLKGADWIPGLAEPGKIDGKQFGIPWYAANRVVVYNKDLFT
                     QAGITTPPKTRDEWIADTTKLNTGGNDGIYLTGQTWYALAGFVWDEGGEFAVKDGDKWK
                     GTLDTPQAQAGMAFYKKLQALGKGPKDSDEAKPLQAEVFAKGKSAQIISVPGGAAAIIK
                     ANPAMKDKIGFFPIPGKTADKPGAVFTGGSDLIIPEASQHQDAAYEVVKALAGEKWQTD
                     MAKEMSYVPNRTSLAGVLAGNEGGAVMAVGAANGHGTPNSPNWAAVEANNPIKQYMTQY
                     LTGADPATAARTASEAIAKALNTAS"
     misc_feature    complement(2468..2483)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.63"
                     /tool="antismash"
     gene            2698..3540
                     /locus_tag="C8250_RS32365"
                     /old_locus_tag="C8250_032375"
     CDS             2698..3540
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064255.1"
                     /locus_tag="C8250_RS32365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032375"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_138358078.1"
                     /transl_table=11
                     /translation="MGSESPRSALKRERVRDYLLELVESHVPGDPIPSERTLCEQLSVS
                     RPTLRSAVDELVNTGLIVREHGRGMFVAPAKITQELVADEHSFSLPKADGTWSSKVLEL
                     TTVQAGARVGRKLHISPAAEITYIARLRLVDGEPMAIEYLHVPALLVPGLRPADMESGD
                     FYRFLRDKHRVRVHEAVQSIEPTVTNEVEARLLGVPLLSPALLFERLTKDETGRPVEYV
                     HSLYRGDRYRIVSRLALGEAGAPGPDAAVRHHPGIPPGDLAGRGDVPSSMVGDVQPAV"
     gene            complement(3578..3892)
                     /locus_tag="C8250_RS32370"
                     /old_locus_tag="C8250_032380"
     CDS             complement(3578..3892)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671007.1"
                     /locus_tag="C8250_RS32370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032380"
                     /product="SAV_915 family protein"
                     /protein_id="WP_138358080.1"
                     /transl_table=11
                     /translation="MPRSRAEEPGNDLPAGPLYVPVRRGPAGFALRLCRTPLGSRTAVA
                     FTSEQRLRSVMGAGQAWIRLSEPAVRALSAPLGATTVTVDPLLTARRVRDPEAVAVALC
                     "
     gene            complement(4099..4404)
                     /locus_tag="C8250_RS43825"
     CDS             complement(4099..4404)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C8250_RS43825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_244809490.1"
                     /transl_table=11
                     /translation="MSGPTGPTPGRGRGHLPGEDVAHAGGGPLGRAIAEDRLDDYEARR
                     RPVAERVVAFTGRTTRIATTRSRVLRTARNLALPVVSRIPAFRTRPATELAELNYR"
     gene            4391..4555
                     /locus_tag="C8250_RS43830"
     CDS             4391..4555
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /locus_tag="C8250_RS43830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_244809491.1"
                     /transl_table=11
                     /translation="MGPDTARRAGLVSAQLLGLALTRYLLRLPAVAALTPDEIETAYAP
                     AIAGVLGLG"
     tRNA            4816..4900
                     /anticodon=(pos:7244244..7244246,aa:Ser,seq:gct)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="C8250_RS32380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="C8250_032390"
                     /product="tRNA-Ser"
     gene            4816..4900
                     /locus_tag="C8250_RS32380"
                     /old_locus_tag="C8250_032390"
     tRNA            4920..5011
                     /anticodon=(pos:7244353..7244355,aa:Ser,seq:cga)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="C8250_RS32385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="C8250_032395"
                     /product="tRNA-Ser"
     gene            4920..5011
                     /locus_tag="C8250_RS32385"
                     /old_locus_tag="C8250_032395"
     tRNA            5016..5095
                     /anticodon=(pos:7244445..7244447,aa:Tyr,seq:gta)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="C8250_RS32390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="C8250_032400"
                     /product="tRNA-Tyr"
     gene            5016..5095
                     /locus_tag="C8250_RS32390"
                     /old_locus_tag="C8250_032400"
     gene            complement(5166..5627)
                     /locus_tag="C8250_RS32395"
                     /old_locus_tag="C8250_032405"
     CDS             complement(5166..5627)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="C8250_RS32395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="C8250_032405"
                     /product="hypothetical protein"
                     /protein_id="WP_138357437.1"
                     /transl_table=11
                     /translation="MGKRQLGQYPQSRYTHLLSSERNSNYTQHRPATFEIPVERPSKGC
                     QSRTLACETCRTSLTYTVFSIPATRARRWAWLLTTLAGIASMLVSVLAIHHLGGEHPGE
                     PGEGVSGLLTLGSIGGFIVAAIGLSFWWYEDGVRGPGRLMGIGGHTIKR"
     misc_feature    complement(5610..5612)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5759..6283)
                     /locus_tag="C8250_RS43835"
     CDS             complement(5759..6283)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="C8250_RS43835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_244809493.1"
                     /transl_table=11
                     /translation="MYVVAFTDHHDRASAAGWILAAHAAGSAIGGLLYGRIAWRRPAAG
                     RLPYLLAALALLFALTGAAPTVVLFALGIAAAGTVVSPTMATAYLAADELAPPGTATRA
                     TAWVNTGVNAGSSLGSGTAGLLVDRLPLPLCFLLAAAPPLLLALGAYPRSGRDARGRSE
                     APLAKASEGRA"
     gene            complement(<6361..6984)
                     /locus_tag="C8250_RS44945"
                     /pseudo=""
     CDS             complement(<6361..6984)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="C8250_RS44945"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSSPRPAASYAAVLRVPHARRTFAAALLGRLSYGIVSLSLLLSLA
                     AATGSYAVAGMITTVFAATSVVLGPARAGLIDRWGARRALPALATPYAVALTALCLATW
                     RPGAPLWLLTFLSAAAGTLTPPLGPTMRALWSALVPDRRLLQRAFSLDTVAEEGLFMTG
                     PLIAVAVRPALGLALSAGLVLIGTLALAASPAAKLLTAREPSVPA"
     gene            complement(7168..8724)
                     /locus_tag="C8250_RS32405"
                     /old_locus_tag="C8250_032415"
     CDS             complement(7168..8724)
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433663.1"
                     /locus_tag="C8250_RS32405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032415"
                     /product="carbohydrate-binding protein"
                     /protein_id="WP_244809885.1"
                     /transl_table=11
                     /translation="MAAAGTFTALAPAAPALALENGLARTPPMGFNNWNTTQCSATFNE
                     AMVKGVADSFVSLGLKDVGYQYVNIDDCWAEKTRDASGNLVPEHTRFPHGIKSVADYVH
                     AKGLKFGLYTSAGTKTCNGTGGFPGGLGHEQQDADLFASWGVDYLKYDNCNNQGVDAQQ
                     RYKAMRDALKKSGRPIVYSICEWGRTDPKVWTWGADVGNLWRTTGDISDNWSSMISRVH
                     TNDDLAAYAGPGHWNDPDMLEVGNGGMTATEYRTHFSLWAMMSAPLLIGSDVRSASAAT
                     QAILKNTDVIALDQDPLGKQATIVSATGGLAVYSKQLANGDRAVALLNETGATATVSTT
                     ASAIGLGGSPSCTLKDLWSKATRSTTGAISASVPSHATVVYRVSRAGTQPDGWYQAEDA
                     AVAQGAVESNHTGYTGTGFVNYDNVTGSYVQWSVTAAQAGPATLTLRYANGTTTGRPMD
                     IAVNSTAVSTGHPFGGTGDWNTWQSASLTVTLKAGANTVRATATTANGGPNVDRLELTQ
                     P"
     gene            8969..9100
                     /gene="pqqA"
                     /locus_tag="C8250_RS32410"
                     /old_locus_tag="C8250_032420"
     CDS             8969..9100
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_030927910.1"
                     /locus_tag="C8250_RS32410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032420"
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="WP_138357438.1"
                     /transl_table=11
                     /translation="MADTTEQTPAQGERPESPAVWSTPEYTVVETALEVTAYSLATR"
     gene            9111..10004
                     /gene="pqqB"
                     /locus_tag="C8250_RS32415"
                     /old_locus_tag="C8250_032425"
     CDS             9111..10004
                     /codon_start=1
                     /gene="pqqB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014669247.1"
                     /locus_tag="C8250_RS32415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032425"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="WP_138357439.1"
                     /transl_table=11
                     /translation="MLLRVLGTAAGGGVPQWNCACPGCSGARRHPGWRRRHASLAVRAA
                     TGRWYIVNATPDIGDQIEDCPELRPGPGARQSPVAGLVLTDAELDHTLGLARLREAGGL
                     QVVSTAPVRRALLTKLRLGEVLTPYTALEWRELGTGAEPLAAGSDVDIDAIPVSGKRPR
                     YAADSAPDAAWVVALRLRDRATGASAVYAPALADWPDALQRAAAAADCVIVDGTFWDDE
                     EPRRTGISERTATGMGHLPIDGPHGTSERLAGLPGRRLYTHLNNTNPLVDPAAPQHKLL
                     AGLGIEVASDGMVIEL"
     gene            10001..10744
                     /gene="pqqC"
                     /locus_tag="C8250_RS32420"
                     /old_locus_tag="C8250_032430"
     CDS             10001..10744
                     /EC_number="1.3.3.11"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR02111"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433953.1"
                     /locus_tag="C8250_RS32420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032430"
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="WP_138357440.1"
                     /sec_met_domain="TIGR02111 (E-value: 1.8e-88, bitscore:
                     294.9, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSAPTTAAGPPATPTDSAIDAGPWSPAAFEARLRAVGEERYHDR
                     HPFNLRMHEGKLSQDELRRWIANRFHYQRHIPVKDALITAKFDTPALRRMWLRRIQDHD
                     GARDGEGGIERWLRLGEAAGLDREELLSGATVLPGVRLAVDGYVNFCRLRPALEAVAAS
                     LTELSAPDLMRTRIDAFERHYRWIEPDGLHYFRTRVTQGRRDSGEALDLVLRWARTRDD
                     QERAVAALGFKCDVLWALLDAVDHA"
     gene            10741..11013
                     /gene="pqqD"
                     /locus_tag="C8250_RS32425"
                     /old_locus_tag="C8250_032435"
     CDS             10741..11013
                     /GO_function="GO:0048038 - quinone binding [Evidence IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PqqD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433952.1"
                     /locus_tag="C8250_RS32425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032435"
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="WP_138357441.1"
                     /sec_met_domain="PqqD_RRE (E-value: 4.2e-15, bitscore:
                     54.2, seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 4.1e-08, bitscore: 32.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTATSDDWRPALAPAILLRRDRVRSTDLLVLPERVVVLSGRAGA
                     IVRLCDGGHSVPQIVGELSACFPGAPVATDVPVFLDRLRAEGWLR"
     aSDomain        10750..11007
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_C8250_RS32425_PqqD_RRE.1"
                     /evalue="1.40E-17"
                     /identifier="RREFam006.1"
                     /label="C8250_RS32425"
                     /locus_tag="C8250_RS32425"
                     /protein_end="89"
                     /protein_start="3"
                     /score="54.2"
                     /tool="antismash"
                     /translation="ATSDDWRPALAPAILLRRDRVRSTDLLVLPERVVVLSGRAGAIVR
                     LCDGGHSVPQIVGELSACFPGAPVATDVPVFLDRLRAEGWL"
     gene            11010..12182
                     /gene="pqqE"
                     /locus_tag="C8250_RS32430"
                     /old_locus_tag="C8250_032440"
     CDS             11010..12182
                     /GO_process="GO:0018149 - peptide cross-linking [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011113086.1"
                     /locus_tag="C8250_RS32430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032440"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="WP_138357442.1"
                     /sec_met_domain="PF04055 (E-value: 3.5e-20, bitscore: 72.0,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 7.9e-06, bitscore: 25.2,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 4.5e-08, bitscore: 32.4,
                     seeds: 166, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 6.5e-09, bitscore:
                     35.0, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPAVPAGSGTSEPEVPRPWALLAELTHACPLHCPYCSNPLELK
                     ARSQELATGDWQDLMRQAGDFGVVHTHLSGGEPLLRPDLEEIVTAAESAGIYTQLVTSG
                     TGLDQARLGALTAAGLRSVQLSVQHADPAASDRIAGRRSFAAKERAAALVRAAGLPLGL
                     NVVLHRDNLDALDAIVELGLAWGVDRIELANAQFYGWGLLNRAALLPAHEQLAVARDAV
                     DRWRTRLGGHPDLVWVVPDYFDGVAKPCMGGWGAVSLTVSPDGTVLPCPAAATLPGLDA
                     PNIRDHTLEWIWDRSPAFNRYRGTAWMSDTCRSCSRRDEDFGGCRCQAFALTGDAARTD
                     PACGLSPDHGLVRAFAREGGADGPLPYVYRYPGRAATGQPDREGVSRSGR"
     gene            complement(12163..12804)
                     /locus_tag="C8250_RS32435"
                     /old_locus_tag="C8250_032445"
     CDS             complement(12163..12804)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019071124.1"
                     /locus_tag="C8250_RS32435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032445"
                     /product="LysE family translocator"
                     /protein_id="WP_138357443.1"
                     /transl_table=11
                     /translation="MVPTDRLLAFAAMSLLLVLIPGPSVLFVLGRALAHGRRTALESVL
                     GNTVGSYVLVVAVSLGLGSLVAGSAAVFLALKLGGAGYLVFLGVKALRHRGDLTMEQNV
                     PGTAAHGGLRTLWEGFVVGVTNPKTMVFFAAVLPQFVDQSAGRIHLQLLILGMVFSLTA
                     LVFDSLWALTASAARTWFARSPRRLAAIGGTGGLAMIGLGVTVAVTGRTD"
     gene            complement(12825..13691)
                     /locus_tag="C8250_RS32440"
                     /old_locus_tag="C8250_032450"
     CDS             complement(12825..13691)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018563095.1"
                     /locus_tag="C8250_RS32440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032450"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_244809497.1"
                     /transl_table=11
                     /translation="MTESGEAVDVRAALLRMRRSGGLPVAFGGLLSGGRQLRISELSGT
                     ETSALRTLAITPGNGLGGKTIAMARPFAVTDYHASRVISHEYDQAVAAEGLRSVLAVPV
                     VVRSRVRGVLYGALRQPMTLGDRPLTAAVDAARELEQSLAVRDEAHRLLAVFHQPVSVD
                     PRAWEEVREAHGELRALAPRITDPRLRQALLTACGRIAAASGAAGRDDAGLDPLPRPAV
                     PVPPLAPREIDVLAAVSSGATNAAAARRLGLRPETVKSYLRSAMRKLGAHTRLEAVVSA
                     RRAGMLP"
     gene            13789..15486
                     /locus_tag="C8250_RS32445"
                     /old_locus_tag="C8250_032455"
     CDS             13789..15486
                     /NRPS_PKS="Domain: AMP-binding (55-455). E-value: 3.6e-84.
                     Score: 274.6. Matches aSDomain:
                     nrpspksdomains_C8250_RS32445_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470039.1"
                     /locus_tag="C8250_RS32445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032455"
                     /product="AMP-binding protein"
                     /protein_id="WP_138357444.1"
                     /sec_met_domain="AMP-binding (E-value: 8.5e-91, bitscore:
                     303.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVTGGTTGATEGPTGEFRAARDFLLRHREEYATAYAGFRWPEPE
                     YFNWALDWFDRIAEDNDRTALWIVEEDGSEGVFSFAELADRSNRVANHLRGLGVRAGDR
                     IVVMLGNQVELWETALAAMKLRAVVIPATPLLGPADLADRIERGGARHVVVRAGDTAKF
                     DGVPGEYTRIVVGGEALDWHPYEEAYASPASFAPDGPTRSADPLMLYFTSGTTARPKLV
                     EQTHLSYPVGHLATMYWIGLQPGDVHLNISSPGWAKHAWSNLFAPWNAEATVFIHNYTR
                     FDAARLMAEMDRCGVTSFCAPPTVWRMLIQADLSALRTPPREVVAAGEPLNPEVIEHVR
                     RSWGVTIRDGFGQTETAVQVANSPGQPLKTGSMGRPCPGYVVVLVDPVTGKPGDEGEIC
                     LDLAHRPVGLMTGYAGDPERTAEAMAGGYYRTGDIGSRDADGYITYVGRSDDVFKASDY
                     KISPFELESVLLEHEAVAEAAVVPAPDPLRLAVPKAYIVLAAGWEPGAETAKAIFAHSR
                     AALAPYKRVRRLEFAELPKTVSGKIRRIELREATARDGSAEYREEDHR"
     aSDomain        13954..15153
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C8250_RS32445_AMP-binding.1"
                     /evalue="3.60E-84"
                     /label="C8250_RS32445_AMP-binding.1"
                     /locus_tag="C8250_RS32445"
                     /protein_end="455"
                     /protein_start="55"
                     /score="274.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="RIAEDNDRTALWIVEEDGSEGVFSFAELADRSNRVANHLRGLGVR
                     AGDRIVVMLGNQVELWETALAAMKLRAVVIPATPLLGPADLADRIERGGARHVVVRAGD
                     TAKFDGVPGEYTRIVVGGEALDWHPYEEAYASPASFAPDGPTRSADPLMLYFTSGTTAR
                     PKLVEQTHLSYPVGHLATMYWIGLQPGDVHLNISSPGWAKHAWSNLFAPWNAEATVFIH
                     NYTRFDAARLMAEMDRCGVTSFCAPPTVWRMLIQADLSALRTPPREVVAAGEPLNPEVI
                     EHVRRSWGVTIRDGFGQTETAVQVANSPGQPLKTGSMGRPCPGYVVVLVDPVTGKPGDE
                     GEICLDLAHRPVGLMTGYAGDPERTAEAMAGGYYRTGDIGSRDADGYITYVGRSDDVFK
                     A"
     CDS_motif       14410..14466
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C8250_RS32445_0001"
                     /evalue="2.30E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="C8250_RS32445"
                     /protein_end="226"
                     /protein_start="207"
                     /score="14.1"
                     /tool="antismash"
                     /translation="MLYFTSGTTARPKLVEQTH"
     CDS_motif       14962..15057
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C8250_RS32445_0002"
                     /evalue="5.10E-04"
                     /label="NRPS-A_a6"
                     /locus_tag="C8250_RS32445"
                     /protein_end="423"
                     /protein_start="391"
                     /score="12.5"
                     /tool="antismash"
                     /translation="PGDEGEICLDLAHRPVGLMTGYAGDPERTAEA"
     gene            15483..17231
                     /locus_tag="C8250_RS32450"
                     /old_locus_tag="C8250_032460"
     CDS             15483..17231
                     /NRPS_PKS="Domain: AMP-binding (58-472). E-value: 7.1e-108.
                     Score: 352.7. Matches aSDomain:
                     nrpspksdomains_C8250_RS32450_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714289.1"
                     /locus_tag="C8250_RS32450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032460"
                     /product="AMP-binding protein"
                     /protein_id="WP_138357445.1"
                     /sec_met_domain="AMP-binding (E-value: 1.7e-111, bitscore:
                     371.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGTGPAEATAANEPPEPSSAEPSSAEPSSAEPSSAEPSYASGTG
                     STPLLGDTIGRNLDRAVAAFPGREALVDVPTGRRWTYTEFGRAVDEVALGLAAKGVARG
                     DRVGIWAVNCPEWVLVQYATARLGAVMVNINPAYRVHELEFVLQQAGIGVLIASTAYKA
                     SDYRAMTEQVRGGCPLLREVVYIGDPGWDELVAGGARLPAGHLAACEAANSCDDPVNIQ
                     YTSGTTGFPKGATLSHHNILNNGYFVGEMIGYTEHDRVCLPVPFYHCFGMVMGNLGATS
                     HGACIVIPAPAFDPVATLHAVERERCTSLYGVPTMFIAELDLPDFATFDLSSLRTGIMA
                     GSPCPVEVMKRVVAEMHMAEVSIAYGMTETSPVSTQTRREDDLERRTATVGRVMPHLEV
                     KVADPATGATVPRGTAGELCTRGYSVMLGYWEEPERTAEAVDAARWMHTGDLAVMRPDG
                     YLSIVGRIKDMIIRGGENVYPREIEEFLYTHPKIADVQVVGVPDERYGEEILACVILRD
                     PADPLTLEELGGFCRGQLAHYKIPRRLRLVDAFPMTVSGKIRKIELREDAIREMSSVVG
                     GEAPPQ"
     aSDomain        15657..16898
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C8250_RS32450_AMP-binding.1"
                     /evalue="7.10E-108"
                     /label="C8250_RS32450_AMP-binding.1"
                     /locus_tag="C8250_RS32450"
                     /protein_end="472"
                     /protein_start="58"
                     /score="352.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="DRAVAAFPGREALVDVPTGRRWTYTEFGRAVDEVALGLAAKGVAR
                     GDRVGIWAVNCPEWVLVQYATARLGAVMVNINPAYRVHELEFVLQQAGIGVLIASTAYK
                     ASDYRAMTEQVRGGCPLLREVVYIGDPGWDELVAGGARLPAGHLAACEAANSCDDPVNI
                     QYTSGTTGFPKGATLSHHNILNNGYFVGEMIGYTEHDRVCLPVPFYHCFGMVMGNLGAT
                     SHGACIVIPAPAFDPVATLHAVERERCTSLYGVPTMFIAELDLPDFATFDLSSLRTGIM
                     AGSPCPVEVMKRVVAEMHMAEVSIAYGMTETSPVSTQTRREDDLERRTATVGRVMPHLE
                     VKVADPATGATVPRGTAGELCTRGYSVMLGYWEEPERTAEAVDAARWMHTGDLAVMRPD
                     GYLSIVGRIKDMIIR"
     CDS_motif       16143..16196
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C8250_RS32450_0001"
                     /evalue="1.30E-05"
                     /label="NRPS-A_a3"
                     /locus_tag="C8250_RS32450"
                     /protein_end="238"
                     /protein_start="220"
                     /score="17.9"
                     /tool="antismash"
                     /translation="IQYTSGTTGFPKGATLSH"
     CDS_motif       16713..16799
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C8250_RS32450_0002"
                     /evalue="8.40E-09"
                     /label="NRPS-A_a6"
                     /locus_tag="C8250_RS32450"
                     /protein_end="439"
                     /protein_start="410"
                     /score="27.8"
                     /tool="antismash"
                     /translation="PRGTAGELCTRGYSVMLGYWEEPERTAEA"
     CDS_motif       16869..16931
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_C8250_RS32450_0003"
                     /evalue="2.00E-03"
                     /label="NRPS-A_a8"
                     /locus_tag="C8250_RS32450"
                     /protein_end="483"
                     /protein_start="462"
                     /score="11.0"
                     /tool="antismash"
                     /translation="VGRIKDMIIRGGENVYPREIE"
     gene            complement(17196..17849)
                     /locus_tag="C8250_RS32455"
                     /old_locus_tag="C8250_032465"
     CDS             complement(17196..17849)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384521.1"
                     /locus_tag="C8250_RS32455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032465"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_138357446.1"
                     /transl_table=11
                     /translation="MENRPGPTGGLRELKKARTRQAISDAAIALFLRHGFDQVPVAEIA
                     AAAEVSKPTLFRYFPTKEDLALHRFADHEGEFARVVRDRPADQAPLTALHRHCAAGLDA
                     RDPVTGLNDTPEVLAYLALLYSAPGLVARLAEYTVRDQEALTGALREASSAAPGDLLPR
                     LAAAQIISVRQVVALANWRKLSDGRSADEVYPEAVAELDQAFGLLGRGLAAHYG"
     gene            17969..19492
                     /locus_tag="C8250_RS32460"
                     /old_locus_tag="C8250_032470"
     CDS             17969..19492
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018553544.1"
                     /locus_tag="C8250_RS32460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032470"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_138357447.1"
                     /sec_met_domain="DAO (E-value: 1e-06, bitscore: 27.6,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSVLIVGAGPTGLTLACELARRGTSVRIVERSPGPQPGSRGKGL
                     NARSLEVFEGMGILDRMRSIGRDDLHFRKYRGGEVVSDTDPFATFSPAPGTPYVRGLLV
                     AQWNVERVLRDRLAEWGVRVEFGTELTGFEQTGDTVTATFAGGERIEVSYLVGCDGGRS
                     GIRKALPVTFGGRTLPEQLMVIGDVEAEGLSQDLWHQWIDEDGGIMLCPLPATSVFQLQ
                     AAPERDAAGLPVEASLESFQRIFDRHARLPGIKLTNPAWISTYRVNERMADRFRVGRVL
                     LAGDAAHVHSIAGGLGMNTGIQDAYNLGWKLALVAAGRAGEGLLDTYEEERLPIAAWTI
                     DTSGELWRQVSRSIRTAGSGTEGVVTPDILGLGLGYRWSSLAVERDAHADDAPLRAGDR
                     APDAPLRDADGAAVRLFEIFSGTHFTLLGFGAGSAAALRESADRHGDAVWTYSIGAGPA
                     GLDDFAGHAAREYGVTGDALVLVRPDGHIALTAPAGDAGAVHDYLGAPA"
     gene            complement(19564..21342)
                     /gene="gcl"
                     /locus_tag="C8250_RS32465"
                     /old_locus_tag="C8250_032475"
     CDS             complement(19564..21342)
                     /EC_number="4.1.1.47"
                     /GO_function="GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="gcl"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986976.1"
                     /locus_tag="C8250_RS32465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_032475"
                     /product="glyoxylate carboligase"
                     /protein_id="WP_187145054.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.7e-46, bitscore:
                     156.9, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2e-31, bitscore:
                     107.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPRMTAARAAVEILKLEGVTDAFGVPGAAINPFYAALKAGGGIDH
                     TLARHVEGASHMAEGYTRANPGNIGVCIGTSGPAGTDMITGLYSAIADSIPILCITGQA
                     PVAVLHKEDFQAVDIASIAKPVTKAATTVLEAAQVPGVFQQAFHLMRSGRPGPVLIDLP
                     IDVQLTEIEFDPETYQPLPVYKPTASRAQIEKAISLLLESERPLIVAGGGIINADASEL
                     LVEFAEMTGTPVIPTLMGWGIIADDHPLNAGMVGLQTSHRYGNATMLESDFVLGIGNRW
                     ANRHTGKLDVYTKGRKFVHVDIEPTQIGKIFAPDYGIASDAKAALELFVEVAKELKAAG
                     RLPDRAEWAASAQHRKATLQRRTHFDNVPIKPQRVYEEMNRAFGPETRYVTTIGLSQIA
                     GAQMLHVYKPRHWINCGQAGPLGWTIPAALGVAKADPEGSVVALSGDYDFQFMLEELAV
                     GAQHKIPYVHVLVNNAYLGLIRQAQRNLDINFQVNLEFENINSPELGVYGVDHVKVVEG
                     LGCKAIRVTEPDELLPAFELAKKLAAEYRVPVVVEAILERITNISMSGSDIASVNEWED
                     IATEPGHAPTSILPLV"
     misc_feature    complement(21449..21465)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.17"
                     /tool="antismash"
ORIGIN
        1 accgacaccg ccacgacgga cagcgcggcg aggttgagcg ggaccttgcg cagcgaccgg
       61 gtcacagggc cgctccttcc tgctggaaca tccggcgcag attgaggacg agcgccacga
      121 gcaggatcag cacggtgagc gtggagaccg ccgagccgac gtcgtacttg tggagcgact
      181 gcgcgatctg gaaggcgtag acgggcaggg tggtggtcgc cccgttcggg ccgccttcgc
      241 tgatcaccca gatctgggcg aagcacttga agcaccagat cacttcgagc gaggtgatca
      301 gggcgaacag ctgccgcagc atggggaagg tgacgtggcg gaagacctgg aagccgccgg
      361 cgccgtccag ccgggccgac tcgtacagct cggcggagat cgtggtgagc ccgccgtaga
      421 gggtcagcgc ggcgaacggc accgactgcc agacgaccag ggtgaccagg atgaagaagg
      481 tcgagttccc gtcggcgaac caggagtagt cgcggtagga gtcgaacccg agctccacca
      541 ggacccagtt gaccactccg aagcgggact ggaagagcca ctggaagatg gtggtggcgg
      601 cgatcaccgg cgtcgcccag gtcagcacga gggcgctcat cacggccagc cgcatccgct
      661 tgccgagccg cgccagcatc agggcgacca gggtgccgat cagcatgatg aggacgacat
      721 tgagcgcggt ccaccacagg gtgcggcgga ccaccgtcca gaactcgggg tcgttgagca
      781 cctccgagta gttgtcgaat ccgacgaagg tggcgccgcc gcggatcagt tcgcccagcc
      841 ggaagtgctg gaaggacatc acggtgttgc ggatcagcgg gtagagcagg aggaaggccg
      901 tgcccaccag ggtgggagcg atcaacagat acggccagac ggcgccgagc acgggccgcc
      961 ggcggcggcg gagggccggg ggcggatcca cggaggcctg ttgagggggg cgccgacggg
     1021 cggcgggggg tgcagcgggc acggcacttc tcctgttgtc ctgttggggt ccgactcgtc
     1081 aggcgggggc ggccacgcgg gcccgcgcgg accgctcccc gttcaggaag cggtgttcag
     1141 cgctttggcg atggcctcgg acgcggtacg ggcggccgtg gccgggtccg ctccggtgag
     1201 gtactgcgtc atgtactgct tgatggggtt gttcgcctcg acagccgccc agttggggga
     1261 gttgggggtg ccgtggccgt tggccgcgcc gaccgccatc accgcgccgc cctcgttgcc
     1321 cgccagcaca ccggccagcg acgtcctgtt ggggacgtag ctcatctcct tggccatgtc
     1381 cgtctgccac ttctcgccgg ccagcgcctt gacgacctcg taggccgcgt cctggtgctg
     1441 cgacgcctcc gggatgatga ggtcggagcc gccggtgaag accgcgccgg gcttgtcggc
     1501 cgtcttgccg gggatcggga agaagccgat cttgtccttc atcgccgggt tggccttgat
     1561 gatcgcggcc gcgccaccgg ggacggagat gatctgcgca ctcttgccct tggcgaagac
     1621 ctccgcctgc agcggcttgg cctcgtcgga gtccttcggg cccttgccca gcgcctggag
     1681 cttcttgtag aacgccatcc cggcctgcgc ctggggcgtg tcgagggtgc ccttccactt
     1741 gtcgccgtcc ttgacggcga actcaccgcc ctcgtcccag acgaagccgg ccagcgcgta
     1801 ccaggtctgc cccgtcagat agatcccgtc gttgccaccg gtgttgagct tggtggtgtc
     1861 ggcgatccac tcgtcgcgcg tcttgggcgg ggtggtgatg ccggcctggg tgaagaggtc
     1921 cttgttgtag accaccacac ggttggcggc gtaccagggg atgccgaact gcttgccgtc
     1981 gatcttgccg ggctcggcca ggcccggtat ccagtccgcg cccttgaggt cggcgacctt
     2041 cccggtcaga tccttgaccc cgccgctggc ggcgtactgc gcgacctggg tgttgccgac
     2101 ctcgatgacg tccggcgcgt ccttgctggc cagagcgctg gtgatcttcg cgccgatacc
     2161 gtcccactcc tggatctgga tcttcagggt ggtaccggag tgggtcgtct cgaaatccgt
     2221 cttgaaccgc tggaggaagg gatcggaaac gctgcccttc atgatccaca cggtgagctc
     2281 ggcggggctc ttgccgtcgc ctgcggtgtc cgacgagtcg gaggaggaac tacaggcgct
     2341 caggccggct acggcgatcg cgagcgccgc ggtgcctgca aggagtctgg ttctcacggg
     2401 gtctgtcacc tcgaatagga gcggcggaac ttaccacttc acaactggtc tggtgctggt
     2461 tggagaaggt ggcatagacc agtgctgccg tcaagaggtc gtgcaggcaa aggcctgacc
     2521 cccggattcc cccggatcac cggtaccggc cgcaactggt ccgcctacgt gacgcgggcc
     2581 ggcctggcgt actggtcagg atctggatag ccttcagggg aggtactgga caggacgcag
     2641 acagaacgct gcccggacgc gccggggcgg accccggacg gcacgaagga gtcaggtatg
     2701 ggaagcgagt cgccacggtc ggcgctcaag cgcgaacggg tgcgcgacta cctgttggag
     2761 ctcgtcgaat cccatgtgcc cggcgacccg atcccctccg agcggaccct gtgcgagcag
     2821 ctgtcggtct cccgcccgac gctgcgctcc gccgtcgacg aactggtcaa caccgggctg
     2881 atcgtccgcg agcacgggcg cgggatgttc gtcgcgcccg ccaagatcac ccaggagctg
     2941 gtggcggacg agcactcgtt ctcgctgccg aaggccgacg gcacctggtc cagcaaggtc
     3001 ctggagctga ccaccgtcca ggccggtgcc agggtgggcc gcaagctgca catctcaccc
     3061 gcggccgaga tcacctacat cgcccggctc cggctcgtcg acggcgagcc gatggccatc
     3121 gagtacctgc acgtgcccgc cctgctcgtg ccgggtctgc ggccggcgga catggagtcc
     3181 ggggacttct accggttcct gcgcgacaag caccgggtgc gggtccacga ggcggtccag
     3241 tccatcgagc cgaccgtcac caacgaggtc gaggcccggc tgctgggcgt gccgctgctt
     3301 tccccggccc tgctcttcga acgtctcacc aaggacgaga ccggccgccc ggtcgagtac
     3361 gtccactcgc tctaccgcgg tgaccgctat cggatcgtct cccggctcgc tctcggcgag
     3421 gccggggctc cggggccgga cgcggccgtc cggcaccacc ccggcatccc gccgggcgat
     3481 ctggcgggcc gcggcgacgt cccctcctcg atggtgggcg acgttcagcc cgcggtgtga
     3541 gcgcgggtcg ttgcgggggt ggtggacgcg gccgtcgtca gcacagggcg acggcgacgg
     3601 cctccgggtc ccgcacccgg cgggcggtga gcagtgggtc gacggtcacc gtggtggcgc
     3661 ccagcggcgc cgacagcgcc cggaccgcgg gctcggagag ccggatccac gcctgcccgg
     3721 cgcccatgac cgaccgcagc cgctgctcgc tggtgaaggc gacggccgtc ctcgaaccga
     3781 gcggcgtacg gcacagccgc agcgcgaaac cggcgggtcc gcgccgtacg ggtacgtaca
     3841 gcgggcccgc cgggagatcg ttcccgggct cttctgcgcg gctgcgcggc attgcggtcc
     3901 tccgggggag cggacgggtg gttccggcgc cggggcggcc gggcggggcg gggggcatcg
     3961 gtgcggatgc cgccccgacg ttcccgacgg tatcgccccc acggtgcgca ctcacctcgc
     4021 gaaccggtcg tcgatgcccg gttgaccctg aaccgggggg aattgacacg gggccgccat
     4081 ccgccggccg gccaccggtc agcggtagtt cagctcggcc agttcggtgg ccggccgggt
     4141 acggaaggcc gggatgcggc tgacgaccgg cagggccagg ttgcgcgccg tgcggagcac
     4201 gcggctccgg gtggtggcga tccgggtcgt ccggccggtg aacgccacga cccgctcggc
     4261 gaccgggcgg cgccgcgcct cgtagtcgtc cagccggtcc tccgcgatgg cccggccgag
     4321 cgggccgccg cccgcatgcg cgacatcttc gcccggcagg tggccccgcc ctcgccccgg
     4381 cgttggcccc gtgggccccg acaccgcccg ccgggcgggc ctggtctccg cccagctcct
     4441 cggcctcgcc ctcacccgct acctcctgcg cctgcccgcc gtcgccgccc tcaccccgga
     4501 cgagatcgag accgcctacg cgccggcgat cgccggcgtc ctggggctcg gctgagcccg
     4561 gcggataatc cgttgccctg gcggtcggcg cccggcacag tggtggcatc accaacgagg
     4621 cgagagggga gcgcacgatg aacgtccagg ccggtctgtc gcaggcgtat cagcgctctt
     4681 ccagtacccc ttcccagcag gggagccccg ggcaggccac gtagtcatcg gtgacggatg
     4741 tcgccgggcc gcccgcggga attcccgcca gggcggcttt ttcgtggccg cccgtctgct
     4801 gtgtccttgc agctgggaga gttggccgag tggtaaggca gcggcttgct aagccgtagt
     4861 cgggtgcgag cccgcgcgcg ttcgatccgc gcactctccg cgtacgtcct gcagttcatg
     4921 gaggagccag ccgaaaggcg gcgccggccg cggtctcgaa aaccgttggg gtgcgcaagc
     4981 atccgtgtgg gttcgacccc cacctcctcc gtcccgggcg aactgggaat cggtgatccc
     5041 aagggcctgt aaagcccccg cttcggctgt ggaggttcga atcctccttc gcccacccgg
     5101 aacgacgcac gcggaacgac gcacgccact gcggcgcgcc acggttacga ggccggggcg
     5161 cgccgtcagc gtttgatggt gtggccgccg atgcccatca gccggccggg gccgcggaca
     5221 ccgtcctcgt accaccagaa gctgagaccg atggcggcga cgatgaagcc gccgatcgag
     5281 ccgagggtga ggagtccgct gacgccctcg ccgggctcgc cggggtgctc gccgccgagg
     5341 tggtggatgg ccaggaccga gacgagcatc gaggcgatgc ccgccagcgt ggtgagcagc
     5401 cacgcccagc gccgggccct ggtcgcgggg atgctgaaga ccgtgtacgt gagcgacgta
     5461 cggcaggtct cacaggcgag ggtgcgcgac tggcagccct tcgagggccg ttcgacggga
     5521 atctcgaagg tcgccggccg gtgctgtgtg tagttgctgt tacgttcgga cgagagcaga
     5581 tgtgtgtacc gggactgggg atattgtcct aattggcgct tgcccaccgc agctccttga
     5641 cgtctggtac tgcgggccac agtaagcgcc aacatgcctg tggcataggg aagttgacga
     5701 ttgaagtacg cggcgggcga ggtatgggcg gacacgcgag gtatcggccc gggacccgct
     5761 acgcccggcc ttcggaagcc ttcgcgagcg gcgcctcgct ccggccccga gcgtcccgcc
     5821 cggaccgggg atacgcgccg agcgcgagca gcagcggagg cgcggccgcc agcaggaagc
     5881 acagcggtag cggcagccgg tcgacgagga gcccggcggt gccggagccc agcgaggagc
     5941 ccgcgttgac acccgtgttg acccaggcgg tggcgcgggt ggcggtcccc ggcggggcga
     6001 gttcgtcggc cgcgaggtac gcggtcgcca tggtggggga gacgacggtg ccggcggcgg
     6061 cgatgccgag ggcgaagagg acgacggtcg gggcggcgcc ggtgagggcg aagagcaggg
     6121 cgagggcggc gagcagatac ggcagccggc cggccgccgg gcgccgccag gcgatccggc
     6181 cgtagaggag cccaccgatc gcgctgcccg ccgcgtgcgc ggcgaggatc cagccggcgg
     6241 cggaggcgcg gtcgtggtgg tcggtgaagg cgacgacgta caactccagg gcgcccaggc
     6301 agcagccgag cccggccgcc gcgtacgccg ccttgcggac cccgccgacc gcagccgcgc
     6361 ggccgggacg gagggctcgc gcgcggtgag cagcttcgcg gccggggacg ccgccagggc
     6421 gagagtgccg atgaggacca ggccggcgga cagggcgagg ccgagcgcgg ggcggacggc
     6481 gacggcgatc agcgggccgg tcatgaacag gccctcctcc gccaccgtgt ccaggctgaa
     6541 cgcccgctgc agcaggcgcc tgtccggaac gagggcgctc cacagcgccc gcatggtcgg
     6601 gccgagcggc ggggtgaggg tgcccgcggc cgccgacagg aaggtgagca gccagagcgg
     6661 ggcacccggc cgccaggtgg ccaggcagag cgcggtcagc gccaccgcgt acggggtggc
     6721 gagggcgggc agggcgcgcc gtgcccccca gcggtcgatg agtccggcac gggccgggcc
     6781 gaggacgacg ctggtcgcgg cgaagaccgt ggtgatcatg ccggcgacgg cgtacgagcc
     6841 ggtcgccgcg gcgagcgaga gcagcaggga gagggagacg atcccgtagg agagccggcc
     6901 caacagggcg gcggcgaagg tgcggcgggc atgggggacc cgcaggacgg cggcgtagga
     6961 cgccgccggg cgtggcgaag acatgggttt tgttcctcag gcagaggcgg gcaggccggc
     7021 gcgaagggcg ggctgcggcc taggcacgag aggtgaggaa catgcgcgcg agtgtagcgc
     7081 acggcgggcg ccgggaccat ggcgcccacc gcgcgggaag gcggtggcgg gcgccatggt
     7141 ccggtcctgc ggagcgtccc gtgaccgtta tggctgggtg agttccagcc ggtccacgtt
     7201 gggtccgccg ttcgcggtgg tcgcggtcgc gcggacggtg ttcgcgccgg ccttgagggt
     7261 gaccgtcagg gacgcgctct gccaggtgtt ccagtcgccg gtcccgccga acggatgccc
     7321 ggtggagacc gccgtgctgt tgacggcgat gtccatcggc cggccggtcg tcgtcccgtt
     7381 cgcgtagcgg agcgtgagtg tggcgggccc cgcctgcgcg gcggtcaccg accactggac
     7441 gtaacttccg gtcacgttgt cgtagttgac gaaaccggtg ccggtgtagc cggtgtggtt
     7501 ggactccacc gcgccctgcg cgacggcggc gtcctcggcc tggtaccagc cgtccggctg
     7561 cgttccggca cgggagaccc ggtagacgac ggtggcgtgc gacggcacgg acgcgctgat
     7621 cgcgccggtc gtggagcggg tggccttgga ccagaggtcc ttgagcgtgc acgagggcga
     7681 gccgccgagg ccgatcgccg aggcggtggt ggagaccgtc gccgtggcgc cggtctcgtt
     7741 gagcagcgcc accgcccggt cgccgttggc gagctgcttg ctgtagacgg ccaggccgcc
     7801 ggtggcggag acgatggtgg cctgcttgcc caacgggtcc tggtccaggg cgatgacgtc
     7861 ggtgttcttc aggatggcct gggtggcggc cgacgccgag cggacatcgc tgccgatcag
     7921 cagcggcgcc gacatcatgg cccacaggct gaagtgggtg cggtactcgg tggcggtcat
     7981 accgccgttg ccgacctcga gcatgtcggg gtcgttccag tggcccggcc ccgcgtacgc
     8041 ggcgagatcg tcattggtgt gcaccctgct gatcatgctc gaccagttgt cgctgatgtc
     8101 gccggtggtg cgccagaggt tgccgacgtc ggcgccccag gtccacacct tggggtcggt
     8161 gcggccccac tcgcagatgc tgtagacgat cggccggccg gacttcttga gcgcgtcgcg
     8221 catcgccttg tagcgctgct gcgcgtcgac gccctggttg ttgcagttgt cgtacttgag
     8281 gtagtccacg ccccaggaag cgaacaggtc ggcgtcctgc tgctcgtggc cgagcccgcc
     8341 ggggaagccg ccggtgccgt tgcaggtctt ggtgcccgcg ctggtgtaga gcccgaactt
     8401 gaggcccttg gcatgcacgt agtcggcgac ggacttgatg ccgtgcggga agcgggtgtg
     8461 ctccggcacg aggttgccgc tcgcgtcacg ggtcttctcg gcccagcagt cgtcgatgtt
     8521 gacgtactgg tagcccacgt ccttgaggcc gagcgatacg aagctgtcgg cgacgccctt
     8581 gaccatcgcc tcgttgaagg tggcgctgca ctgagtggtg ttccagttgt tgaagcccat
     8641 cggtggcgtg cgggccaggc cgttctccag ggcgagcgcg ggggcggcgg gggcgagggc
     8701 ggtgaacgtt ccggcggcgg cgatggtcgc ggtggccagt acggagcaga gcaggcggtg
     8761 cggtctcacg gcatcctcct cgggtcggca ccggaaaggt aggagcctgc cgagtaatcg
     8821 tcaagaacct ttacagatag cagatcggat gaatacgctc cgattgatgg cgctgctgcc
     8881 tgctgtagca gtactgctgt cacacgcgca ccaactgatc ggatctatcc acgcgaggac
     8941 atcacgacag gaaggacggt gcacagccat ggccgacacg acggaacaga ccccggctca
     9001 gggggagcga ccggagagcc cggcggtctg gagtacgccc gagtacacgg tcgtcgagac
     9061 cgcgctcgaa gtgaccgcct actcgctcgc cactcgctga gtccgccgcc gtgctgctgc
     9121 gggtactcgg taccgcggcg ggcggcggcg tcccccagtg gaactgcgcg tgccccgggt
     9181 gctccggggc acgccgccac ccggggtggc gccgccgcca cgcgtcgctc gcggtccgcg
     9241 cggccaccgg ccgctggtac atcgtcaacg cgacccccga catcggcgat cagatcgagg
     9301 actgcccgga gctgcgcccc ggacccggcg cgcggcagtc gccggtcgcc ggactcgtcc
     9361 tcaccgacgc cgaactcgac cacaccctcg gcctcgcccg gctccgtgag gccggcggac
     9421 tgcaggtggt gtcgaccgcg cccgtacgac gggccctcct gacgaagctg cgcctgggcg
     9481 aggtgctcac gccgtacacc gctctggaat ggcgggagtt ggggaccggc gcggagccgc
     9541 tggccgccgg ctcggacgtg gacatcgatg ccatcccggt gtccgggaag cggccgcgct
     9601 acgccgccga cagtgctccc gacgccgcct gggtcgtcgc gctccggctg cgcgaccgcg
     9661 ccaccggcgc ctccgccgtc tacgcccccg ccctcgccga ctggcccgac gctctccagc
     9721 gggccgccgc cgcagccgac tgcgtcatcg tcgacggcac cttctgggac gacgaggagc
     9781 cccgccgcac cggtatctcg gagcgcaccg ccaccggcat gggacatctg ccgatcgacg
     9841 gcccgcacgg cacctcggaa cgcctcgccg gcctgccggg acgccgcctg tacacccacc
     9901 tcaacaacac caaccccctc gtggatccgg ccgcgccgca gcacaagctg ctggccgggc
     9961 tgggcatcga ggtggcctcc gacgggatgg tgatcgagct atgacgtcag cgcccacgac
    10021 agcagcgggg ccgcccgcaa cgcccacgga ctccgccatc gacgccgggc cctggagccc
    10081 ggccgcattc gaggcacggc tgcgcgcggt gggggaggag cggtaccacg accgccaccc
    10141 cttcaacctg cggatgcacg aaggaaagct cagccaggac gagttgcgcc gttggatcgc
    10201 caaccgcttc cactaccagc ggcacatccc cgtcaaggac gccctgatca cggcgaagtt
    10261 cgacaccccc gcactgcggc ggatgtggct gcgccgtatc caggaccacg acggcgcgcg
    10321 ggacggcgag ggcggcatcg aacgctggct ccgcctcggc gaggccgcgg gactcgaccg
    10381 tgaggaactg ctctccggcg ccacggtgct gcccggggta cgcctcgcgg tcgacgggta
    10441 cgtcaacttc tgccggctgc gcccagcgct cgaagccgtc gcggcctcgc tcaccgaact
    10501 gtccgccccc gacctgatgc gcacccgcat cgacgccttc gaacggcact accgctggat
    10561 cgaaccggac gggctgcact acttccgcac ccgcgtcaca cagggccgcc gggacagcgg
    10621 ggaggcgctc gatctggtgt tgcgctgggc ccgcacccgg gacgaccagg agcgcgcggt
    10681 ggccgcgctc ggcttcaagt gcgatgtgct gtgggcactg ctcgacgccg tggaccacgc
    10741 atgacgaccg ccacgagcga cgactggcgt cccgccctgg cccccgccat cctgctgcgc
    10801 cgcgaccggg tccgctcgac ggacctgctg gtgctgcccg agcgggtcgt cgtgctgagc
    10861 ggccgggcgg gcgccatcgt ccggctctgc gacggcggcc acagcgttcc gcagatcgtc
    10921 ggtgaactct ccgcatgctt ccccggcgca cccgtggcca cggacgtacc cgtattcctc
    10981 gaccggctgc gggcggaggg ctggctgcga tgaccacccc tgccgtcccc gccggatccg
    11041 ggacgtccga acccgaggtg ccgcgcccct gggcgctgct cgccgaactc acccacgcct
    11101 gtccgctgca ctgcccttac tgctccaacc ccctggaact gaaggcccgt tcgcaggagc
    11161 tggcgaccgg ggactggcag gacctgatgc gccaggccgg tgacttcggc gtcgtgcaca
    11221 cccacctgtc cggcggcgag ccgctgctgc ggcccgatct ggaggagatc gtcaccgccg
    11281 ccgaatccgc cggtatctac acgcagttgg tcaccagcgg caccggcctc gaccaggccc
    11341 ggctcggcgc gctgaccgcg gccgggctgc gcagcgtcca gctgtccgtg cagcacgcgg
    11401 accccgcggc ctcggaccgg atcgccggca gacgctcctt cgccgcgaag gaacgggcgg
    11461 ccgcgctggt acgcgccgcc ggactgccgc tcggcctgaa cgtcgttctg caccgcgaca
    11521 acctggacgc cctggacgcc atcgtcgaac tgggcctggc ctggggtgtc gaccggatcg
    11581 aactcgccaa cgcccagttc tacgggtggg gcctgctcaa ccgtgccgcg ctgctgcccg
    11641 cccacgagca gctggctgtc gcgcgggacg cggtcgaccg gtggcgtacc cggctcggcg
    11701 gccaccccga cctggtgtgg gtggtgcccg actacttcga cggcgtcgcc aaaccctgta
    11761 tgggcggttg gggcgcggtc tcactgaccg tctctcccga cggcaccgtc ctgccgtgcc
    11821 cggccgccgc cacgctgccc ggcctggacg cgccgaacat ccgtgaccac accctggagt
    11881 ggatctggga ccggtccccg gccttcaacc gctaccgggg caccgcgtgg atgagcgaca
    11941 cctgccgctc ctgctcccgc cgggacgagg acttcggcgg gtgccggtgc caggccttcg
    12001 ccctgacggg cgacgccgcc cgcacggatc cggcctgtgg tctctccccg gaccacggcc
    12061 tggtccgcgc gttcgcccgg gagggcggag ccgacggacc gctcccgtac gtctaccggt
    12121 atcccgggcg ggcggcgacg ggacagcccg atcgcgaggg ggtcagtcgg tccggccggt
    12181 gaccgcgacc gtgacgccga ggccgatcat cgccagcccg ccggtgccgc cgatcgccgc
    12241 cagccggcgc ggggagcgcg cgaaccaggt gcgggcggcg gaggcggtga gggcccagag
    12301 actgtcgaag accagcgcgg tcaggctgaa caccatgccc aggatcagca gctgcagatg
    12361 gatccggccc gcggactggt ccacgaactg cgggagcacg gcggcgaaga acaccattgt
    12421 cttggggttc gtcacaccga ccacgaaccc ctcccacagg gtccgcagcc cgccgtgggc
    12481 ggcggtgccg gggacgttct gctccatcgt caggtcaccg cggtgccgca gcgccttcac
    12541 cccgaggaac accagatacc cggcgccgcc cagcttcagt gccaggaaca ccgccgcgga
    12601 gcccgccacg agcgagccga gcccgagcga caccgccacg accaggacgt aggagcccac
    12661 cgtgttgccg agcacgctct ccagggccgt gcggcggccg tgcgcgaggg cccggccgag
    12721 gacgaacagc acgctggggc ccggtatcag aaccagcagc agggacatcg cggcgaaggc
    12781 cagcaggcgg tcggtcggga ccatcaccat cacctccgtg gccgtcaggg cagcatccct
    12841 gcccggcgcg cggacactac cgcctccagc cgggtgtgag cacccagttt tcgcatcgcg
    12901 gagcgcagat agctcttgac ggtctccggc cgcagtccaa gccgccgcgc ggcggccgcg
    12961 ttcgtcgcgc ccgaggacac cgcggcgagc acatcgatct cacgcggtgc cagcggcggg
    13021 accggcaccg ccggtcgcgg cagtggatcg aggcccgcgt cgtcccgccc cgccgccccc
    13081 gacgcggcgg cgatccgccc gcaggcggtc agcagcgcct gccgcagccg ggggtcggtg
    13141 atccgcgggg cgagggcgcg cagctcgccg tgcgcctcgc ggacctcctc ccaggcccgg
    13201 gggtcgacgg agaccggctg gtggaagacg gccagcagcc ggtgggcctc gtcgcggacc
    13261 gcgagggact gctccagctc ccgcgccgcg tccacggcgg ccgtcagcgg gcggtcgccg
    13321 agcgtcatgg gctggcgcag cgcaccgtac agcaccccgc ggaccctgct gcggacgacc
    13381 accggcaccg ccaggaccga ccgcagaccc tcggccgcga cggcctggtc gtactcatgg
    13441 ctgatcaccc gcgacgcgtg gtaatcggtg acggcgaacg gccgggccat ggcgatggtc
    13501 ttgccgccca gaccgttgcc cggggtgatc gccagggtgc gcagggcgga cgtctccgtg
    13561 cccgagagct ccgagatccg cagctgccgg ccgcccgaca gcagcccccc gaaggcgacc
    13621 ggcagtccgc cggaccgccg catgcgcagc agggccgccc gtacgtcaac ggcttcgccg
    13681 gattcggtca cggtcactcc ttcagcccga cacccccgtc cgggggtggt gagacccggg
    13741 tcactggatg gacgatgcta ctcagcggtc aggcactgag gaggacacat ggcggtgacc
    13801 ggtgggacga ccggagcgac cgaggggccg accggggagt tccgggcggc ccgcgacttc
    13861 ctgctgcggc accgcgagga gtacgcgacg gcctacgcgg gcttccgctg gccggagccc
    13921 gagtacttca actgggcgct ggactggttc gaccggatcg ccgaggacaa cgaccgaacc
    13981 gcgctgtgga tcgtggagga ggacggctcc gaaggcgtct tctccttcgc ggagctggcc
    14041 gaccggtcga accgtgtcgc caaccacctc aggggcctgg gcgtgcgggc cggtgaccgg
    14101 atcgtcgtga tgctcggcaa ccaggtcgag ctgtgggaga ccgcgctcgc cgccatgaag
    14161 ctgcgcgccg tcgtcatccc cgccacccca ctgctcgggc cggccgacct ggcggaccgg
    14221 atcgagcgcg gcggcgccag gcatgtcgtg gtgcgggccg gggacaccgc caagttcgac
    14281 ggggtgccgg gggagtacac ccgtatcgtc gtcggcggcg aggccctgga ctggcacccg
    14341 tacgaggagg cgtacgcgag ccccgcctcg ttcgcgccgg acggccccac ccggtccgcc
    14401 gacccgctga tgctctactt cacctccggc accaccgccc ggcccaagct cgtcgagcag
    14461 acccacctct cgtaccccgt cgggcatctg gccaccatgt actggatcgg cctgcagccc
    14521 ggggacgtgc acctcaacat ctcctcgccg ggctgggcca agcacgcctg gagcaatctc
    14581 ttcgcgccgt ggaacgccga ggcgacggtg ttcatccaca actacacgcg cttcgacgcg
    14641 gcccggctga tggcggagat ggaccgctgc ggggtcacca gcttctgcgc cccgccgacc
    14701 gtgtggcgga tgctgatcca ggcggacctg agcgcgctgc gcaccccgcc gcgcgaggtg
    14761 gtcgcggcgg gcgagccgct gaaccccgag gtcatcgagc acgtccgccg gtcctggggc
    14821 gtcaccatcc gggacggctt cggccagacc gagacggcgg tgcaggtggc caacagcccc
    14881 ggccagccgc tgaagaccgg ctcgatgggc cgaccgtgcc ccggctacgt ggtggtgctc
    14941 gtcgacccgg tcaccggcaa gccgggcgac gagggcgaga tctgcctgga cctcgcgcac
    15001 cgcccggtgg ggctgatgac cggctacgcg ggtgacccgg agcgcaccgc ggaggcgatg
    15061 gcgggcggct actaccgcac cggcgacatc ggttcgcggg acgccgacgg ctacatcacg
    15121 tacgtcggcc gctccgatga cgttttcaag gcctccgact acaagatctc cccgttcgag
    15181 ctggagagcg tgctgctgga gcacgaggcg gtcgccgagg ccgccgtcgt cccggcgccg
    15241 gacccgctgc ggctcgccgt accgaaggcg tacatcgtgc tcgccgccgg ctgggagccg
    15301 ggcgcggaga ccgcgaaggc gatcttcgcg cactcccgcg cggcgctcgc cccgtacaag
    15361 cgcgtccgcc gcctggagtt cgcggagctg cccaagaccg tctccggcaa gatccgccgt
    15421 atcgagctgc gcgaggccac cgcccgggac ggcagtgccg agtaccgcga ggaggaccac
    15481 cgatgaccgg gacaggaccg gccgaagcga cggccgcgaa cgagccgccc gaaccgtcct
    15541 ctgccgaacc gtcctctgct gaaccgtcct ctgctgaacc atcctctgcc gaaccgtcct
    15601 acgcgagcgg caccggcagc accccgctgc tcggcgacac catcggccgc aacctggacc
    15661 gggccgtcgc ggcgtttccc ggccgcgagg cgctggtcga cgtgccgacc ggccgccgct
    15721 ggacctacac cgagttcggc cgggccgtcg acgaggtggc gctggggctg gcggccaagg
    15781 gcgtggcccg cggcgaccgc gtcggcatct gggcggtgaa ctgcccggag tgggtgctcg
    15841 tccagtacgc gaccgcgcgg ctcggcgccg tcatggtcaa cattaacccg gcctaccggg
    15901 tgcacgagct ggagttcgtc ctccagcagg ccggaatcgg ggtgctgatc gcctcgaccg
    15961 cgtacaaggc cagcgactac cgggcgatga cggagcaggt acgcggcggc tgtccgctgc
    16021 tgcgcgaggt cgtgtacatc ggcgacccgg gctgggacga actggtggcg ggcggggcgc
    16081 ggctgccggc cgggcacctg gccgcgtgcg aggcggcgaa cagttgcgac gacccggtca
    16141 acatccagta cacctcgggc accaccggct tccccaaggg cgcgacgctc tcgcaccaca
    16201 acatcctcaa caacgggtac ttcgtcggcg agatgatcgg ctacaccgag cacgaccggg
    16261 tgtgcctgcc ggtgcccttc taccactgct tcggcatggt gatgggcaat ctgggcgcca
    16321 ccagccacgg cgcctgcatc gtcatcccgg cccccgcctt cgacccggtc gccaccctgc
    16381 acgcggtcga acgggagcgc tgcacctcgc tctacggcgt gccgacgatg ttcatcgccg
    16441 aactggacct gccggacttc gcgaccttcg acctgtcctc gctgcgcacc gggatcatgg
    16501 ccggatcgcc ctgcccggtc gaggtgatga agcgcgtggt cgccgagatg cacatggcgg
    16561 aggtgtccat cgcctacggc atgacggaga cctcgcccgt ctccacccag acccggcgcg
    16621 aggacgacct ggagcgccgc accgccaccg tcggccgcgt catgccgcac ctcgaggtga
    16681 aggtcgccga tccggcgacc ggtgcgacgg tgccgcgcgg tacggcgggc gagctgtgca
    16741 cccgcggcta ttcggtgatg ctgggctact gggaggagcc ggagcggacc gccgaggcgg
    16801 tcgacgccgc gcgctggatg cacaccggcg acctcgccgt gatgcgtccc gacggctatc
    16861 tgtccatcgt cggccggatc aaggacatga tcatccgcgg tggggagaac gtctatccgc
    16921 gggagatcga ggagttcctg tacacccacc ccaagatcgc cgatgtgcag gtcgtcgggg
    16981 tgccggacga gcggtacggc gaagagatcc tggcctgcgt catcctccgc gaccccgcgg
    17041 acccgctcac cctggaggag ctcggcggct tctgccgcgg acagctcgcg cactacaaga
    17101 tcccgcgccg gctgcgcctc gtggacgcct tcccgatgac ggtcagcggc aagatccgca
    17161 agatcgaact gcgggaggac gcgatccggg agatgtcatc cgtagtgggc ggcgaggccc
    17221 cgccccagta gtccgaaggc ctggtcgagc tccgcgaccg cctcgggata cacctcgtcg
    17281 gcgctgcgcc cgtccgacag cttccgccag ttggcgaggg cgacgacctg gcgcacggag
    17341 atgatctgcg ccgccgcgag gcggggcagc aggtcgccgg gcgcggccga cgaagcctcc
    17401 cgcagtgctc cggtcagcgc ctcctggtcg cggacggtgt actccgccag ccgtgccacc
    17461 aggccgggcg ccgagtagag cagcgcgaga tacgccagca cctctggggt gtcgttgagc
    17521 ccggtgaccg gatcacgggc gtcgaggccc gccgcgcagt gccggtgcag cgcggtgagc
    17581 ggtgcctggt ccgcggggcg gtcgcgcacc acccgggcga actcgccctc gtggtcggcg
    17641 aaccggtgca gcgccagatc ctccttggtg gggaagtacc ggaagagcgt cggcttggac
    17701 acctcggcag cggcggcgat ctcggcgacc ggcacctggt cgaagccgtg ccggaggaac
    17761 agtgcgatgg cggcgtccga gatggcctgc cgggtcctgg ccttcttcag ttcccgcagt
    17821 ccgcccgttg gtcctggccg gttctccatg gcgctcatcg tagccgaagt gcaatcaagt
    17881 caaaaacgtt actcggttgc atatgaaacc cggtcatggt tgcatcaccg tcagacgaac
    17941 gcggacgagc tcgatgagag gacagcggat gaccagcgtg ctgatcgtgg gagcgggacc
    18001 gaccgggctg acgctggcct gtgaactggc ccggcgcggc acctccgtcc ggatcgtgga
    18061 aaggtcccct ggaccccagc cgggttcccg gggcaagggg ctcaacgcgc gcagcctgga
    18121 ggtcttcgag ggcatgggga ttctcgaccg catgcggtcg atcgggcgcg acgacctgca
    18181 cttccgcaag taccggggcg gcgaggtggt ctccgacacc gatcccttcg cgaccttctc
    18241 cccggcgccc ggcaccccgt acgtacgcgg gctgctcgtc gcgcagtgga acgtcgagcg
    18301 ggtgctgcgg gaccggctcg ccgagtgggg cgtgcgcgtc gagttcggca ccgaactgac
    18361 ggggttcgag cagaccggcg acacggtgac cgccacgttc gcgggcggtg agcggatcga
    18421 ggtgtcctac ctggtgggct gcgacggcgg ccgcagcggg atccgcaagg cgctgccggt
    18481 caccttcggg ggccggacgc tgccggagca gctgatggtc atcggtgatg tcgaggccga
    18541 gggcttgagc caggacctgt ggcaccagtg gatcgacgag gacggcggca tcatgctctg
    18601 cccgctcccc gccacctcgg tgttccagct ccaggcggcc cccgagcggg acgcggcggg
    18661 gctcccggtg gaggcgtcgc tggagagctt ccagcggatc ttcgaccggc acgcgcggct
    18721 ccccggcatc aagctcacca accccgcctg gatctccacc taccgcgtca acgagcggat
    18781 ggccgaccgt ttccgggtcg gccgggtcct cctcgccggg gacgccgccc atgtgcactc
    18841 catcgcgggc ggcctcggga tgaacaccgg tatccaggac gcctacaacc tcggctggaa
    18901 actcgcgctg gtggccgcgg gccgggccgg cgaagggctg ctcgacacct acgaggagga
    18961 gcggctgccg atcgccgcct ggaccatcga caccagcggt gagctgtggc gccaggtctc
    19021 gcgctcgatc aggaccgcgg gcagtgggac ggagggcgtg gtcacgcccg acatcctcgg
    19081 cctggggctg ggctaccgct ggagctcgct ggccgtcgag cgcgacgccc atgccgacga
    19141 cgctccgctg cgcgccggcg accgcgcgcc cgacgccccg ctgcgggacg ccgacggtgc
    19201 cgccgtccgg ctcttcgaga tcttcagcgg cacccacttc acgctgctcg gcttcggcgc
    19261 cggcagcgcg gcggcgctcc gggagagcgc ggaccggcac ggcgacgcgg tgtggaccta
    19321 ctcgatcggt gcgggcccgg cgggcctgga cgacttcgcg gggcacgcgg cccgcgagta
    19381 cggcgtcacc ggcgacgccc tggtgctggt ccgcccggac ggccacatcg cgctcaccgc
    19441 cccggccggt gacgccggtg cggtgcacga ctacctcggc gcgccggcct agggccggaa
    19501 cagaacacga ccgcacccca ggccgtcctc cgggggtgcg gtcgtgcggt cggtggagcg
    19561 ggatcagacg agcggcagga tcgaggtcgg ggcgtggccc ggctcggtcg cgatgtcttc
    19621 ccactcgttg acgctggcga tgtcgctgcc gctcatcgag atgttggtga tgcgctccag
    19681 gatcgcctcg acgaccaccg ggacgcggta ctccgcggcg agcttcttgg ccagctcgaa
    19741 ggcgggcagc agctcgtccg gctcggtgac ccggatcgcc ttgcagccca ggccctcgac
    19801 gaccttgacg tggtcgacgc cgtagacgcc cagctccggg gagttgatgt tctcgaactc
    19861 caggttgacc tggaagttga tgtcgaggtt gcgctgcgcc tggcggatca ggcccaggta
    19921 cgcgttgttg accaggacgt ggacgtacgg gatcttgtgc tgcgcgccga ccgccagctc
    19981 ttcgagcatg aactggaagt cgtagtcgcc ggagagcgcg acgaccgagc cttcggggtc
    20041 ggccttggcg acacccagcg cggccgggat ggtccagccg agcgggccgg cctggccgca
    20101 gttgatccag tgccgcggct tgtagacgtg cagcatctgc gcgccggcga tctgggagag
    20161 gccgatggtg gtgacgtacc gggtctccgg gccgaaggcg cggttcatct cctcgtagac
    20221 gcgctgcggc ttgatcggca cgttgtcgaa gtgcgtccgg cgctgcaggg tggccttgcg
    20281 gtgctgggcg gacgcggccc actcggcgcg gtcgggcagc ctgcccgcgg ccttgagctc
    20341 cttggcgacc tcgacgaaga gctccagcgc ggccttggcg tccgacgcga ttccgtagtc
    20401 cggcgcgaag atcttgccga tctgggtggg ctcgatgtcg acgtggacga acttgcggcc
    20461 cttggtgtag acgtccagct tgccggtgtg gcggttggcc cagcggttgc cgatgcccag
    20521 gacgaagtcc gactcgagca tcgtcgcgtt gccgtagcgg tgcgaggtct gcaggccgac
    20581 catgccggcg ttcagcgggt ggtcgtcggc gatgatgccc cagcccatca gggtggggat
    20641 gaccggggtg ccggtcatct cggcgaactc gaccagcagc tcggaggcgt cggcgttgat
    20701 gatgccgccg ccggcgacga tcagcgggcg ctcggactcg agcagcagcg agatcgcctt
    20761 ctcgatctgg gcgcgcgatg cggtcggctt gtagaccggc agcggctggt acgtctcggg
    20821 gtcgaactcg atctcggtga gctggacgtc gatcggcagg tcgatgagga ccgggccggg
    20881 ccgtcccgag cgcatcaggt ggaacgcctg ctggaagacg ccggggacct gtgcggcctc
    20941 caggaccgtg gtggcggcct tggtgaccgg cttggcgatc gaggcgatgt cgacggcctg
    21001 gaagtcctcc ttgtggagga ccgcgaccgg ggcctggccg gtgatgcaga ggatcgggat
    21061 cgagtccgcg atggccgagt agagcccggt gatcatgtcg gtaccggcgg ggccggacgt
    21121 accgatgcag acaccgatgt tgcccgggtt ggcacgggtg tagccctcgg ccatgtggga
    21181 cgcgccctcg acgtggcggg cgagcgtgtg gtcgatgccc ccgccggcct tgagcgcggc
    21241 gtagaagggg ttgatcgcgg cgcccggcac accgaacgcg tcggtgacgc cctcgagctt
    21301 gaggatctcc acggccgcac gggcggcagt catacgaggc atcgagtact cctgggtcgg
    21361 actgtcgggg tgggctcttg ctgcgaggcc agggcgcccg gttttccgta gtgctagttt
    21421 ccgtaatgcg gaagtctagt tctgctatat ggaagtaatc tagaacgagg ctttccgccc
    21481 gtcaaggcag tacctgttcc ggacctgttc cggacgcgga agagcgggct gccatccggc
    21541 agcccgctct cggtacgcgt gaagtcgctg gaggtcaggg gatgacgggc cccgtccgca
    21601 tgagcgtgtc ggccgcgtcg ttgaccggct gcggagtgcc cgtcagatcc atcacgaaga
    21661 gcgggacgcc caactggtcg gcgcgcagcc gcgcgtcgtg cgcgtagccg gccagggaga
    21721 agaaggcgcc gacggagtcc tcgttcaggc agttgagcca caggcactcg atgtcgcgca
    21781 gcgcggtggg ctgggtggtg gggtcgatct gggccacgag gtcgtcgccg cgcagatcga
    21841 tgccgttggc ggcgtggtct ccggccactc gtatgccggc gaagccgagc cacttcaggt
    21901 actgggcggc ggcggtgagc gcgtcacggg cggtgcggat ggtgaccggg cggaacggcg
    21961 gccgtggcgc gggcggcggc gggccgggga ccgcgggcgg cgaaggggcg gcggacagcg
    22021 ggcgcagggc cgcggtggcc gggccgggag gagcgagtgc cgcggtgtcg accggcatcc
    22081 ggatcaccga accgcagtcg cagccgaact cgggctgcgg ccactggtcc tgccggccgc
    22141 agtcggggca gcgcatgctc acccaggagt cgtcccacga ac
//
