LOCUS       NZ_CP048835            10486 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. So13.3 chromosome, complete genome.
ACCESSION   NZ_CP048835
VERSION     NZ_CP048835.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. So13.3
  ORGANISM  Streptomyces sp. So13.3
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Quezada-Solis,D., Khalil,Z.G., Capon,R.J.,
            Andreote,F.D. and Barrientos,L.
  TITLE     Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed
            Culture and Elicitation Conditions for the Production of
            Antimicrobial Compounds
  JOURNAL   Biomolecules 10 (5), 673 (2020)
   PUBMED   32349314
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Lamilla,C., Abanto,M., Maruyama,F., Jorquera,M.A.,
            Santos,A., Martinez-Urtaza,J. and Barrientos,L.
  TITLE     Antarctic Streptomyces fildesensis So13.3 strain as a promising
            source for antimicrobials discovery
  JOURNAL   Sci Rep 9 (1), 7488 (2019)
   PUBMED   31097761
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K. and Barrientos,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Centro de Excelencia en Investigacion
            Biotecnologica Aplicada al Medio Ambiente (CIBAMA), Universidad de
            La Frontera, Av. Francisco Salazar 01145, Temuco, La Araucania
            4811230, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2020
            Assembly Method                   :: Unicycler v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140.0x
            Sequencing Technology :: Illumina MiSeq; Illumina HiSeq; Oxford
            Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005860785.2-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:03:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,707
            CDSs (total)                      :: 8,616
            Genes (coding)                    :: 8,274
            CDSs (with protein)               :: 8,274
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 109 of 342
            Pseudo Genes (incomplete)         :: 268 of 342
            Pseudo Genes (internal stop)      :: 24 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:45:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5141099
            Orig. end                         :: 5151585
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Laboratorio de Biologia
            Molecular Aplicada, Universidad de La Frontera, Avenida Alemania
            0458, Temuco, Chile.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10486
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="13"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10486
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10486
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5486](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5486)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(530..1642)
                     /locus_tag="C8250_RS23155"
                     /old_locus_tag="C8250_023170"
     CDS             complement(530..1642)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136819.1"
                     /locus_tag="C8250_RS23155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_023170"
                     /product="PD40 domain-containing protein"
                     /protein_id="WP_138351285.1"
                     /transl_table=11
                     /translation="MSARTRGSTAVAVALTATLTAATAALLTGCGPTSGARAVTTPPAA
                     TNTPSSGSSAAPAGGATPSAGTAPSASASTTPAGGSTPTGTPTTAGSTRAPVLNGTANS
                     KLTISDGTRYVVMNGTRVDFGTAVRDLAWSPDGRKAAFIDGAGNLALANPNGTGRVTVA
                     RNPGGQNWSHPTWQVTAYDSIPELSGRSNILFAVRKSGVSELDSVPSSAVNGTPKKLGL
                     GWEAGDEVKPVPQTGNVWPSAGGTHGSAVYANSGTGEVFIRDDYVRQQGSALTHGSQPA
                     MSHSEHEEVVFVRSVGGHDHLFLENPTDAGPVFKDLTPYATTDYTEPAWAPDGSTIAAR
                     TPAGIVTLSTKSSAAPKLVSTYTGLPSYRG"
     misc_feature    1703..1718
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.39"
                     /tool="antismash"
     gene            1886..2698
                     /locus_tag="C8250_RS23160"
                     /old_locus_tag="C8250_023175"
     CDS             1886..2698
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955679.1"
                     /locus_tag="C8250_RS23160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_023175"
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="WP_232484939.1"
                     /transl_table=11
                     /translation="MTELPWIEEAGKVAPKDARALSKLFFDRLQSLEEGTRDHQYARNT
                     LIELNLSLVRFAAGRFRNRSEDMEDIVQVGTIGLIKAIDRFDLSREVEFTTFAIPYITG
                     EIKRFFRDTSWAVHVPRSLQERRIALAQAKDALSQRMDRSPTTAELAEYLDLTPAEVNE
                     GLVASNGYTAGSLDLPAGENNEEAAGSSFVDRLGEDDPGMEAVENLQALKPLMAELGER
                     DRAILRMRFGQEMTQSEIGAELGVSQMHVSRLLTRTLTRLRTGLLTQA"
     gene            complement(2775..3278)
                     /locus_tag="C8250_RS23165"
                     /old_locus_tag="C8250_023180"
     CDS             complement(2775..3278)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039301.3"
                     /locus_tag="C8250_RS23165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_023180"
                     /product="DUF5949 family protein"
                     /protein_id="WP_138351287.1"
                     /transl_table=11
                     /translation="MSQTSTAPDITRQSQMGTLAVIPWAGEHIEDERDIAFLLAYSLGD
                     VEGGPEAAEAAITAAIEEVGLPIGGTILDIAQVPKAPVKLLVEGGQAVLTMPYLHAACP
                     VPEQWVDAARDRGMVYFMFATRPWPEAAPGKPVSEEVLKSFVGDETMLAGSAHCFVPIG
                     SLQG"
     misc_feature    complement(3374..3389)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.71"
                     /tool="antismash"
     misc_feature    complement(3570..3583)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.75"
                     /tool="antismash"
     gene            3658..3909
                     /locus_tag="C8250_RS23170"
                     /old_locus_tag="C8250_023185"
     CDS             3658..3909
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818707.1"
                     /locus_tag="C8250_RS23170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_023185"
                     /product="chaplin"
                     /protein_id="WP_138351288.1"
                     /transl_table=11
                     /translation="MSRIAKAAAITAAAGAVLAAGAGMAAADAGAQGAAVGSPGVLSGN
                     QAQIPVHVPVNVCGNSVNVIGLLNPAFGNHCENVSGGW"
     misc_feature    3661..3673
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.72"
                     /tool="antismash"
     gene            complement(4112..4981)
                     /locus_tag="C8250_RS23175"
                     /old_locus_tag="C8250_023190"
     CDS             complement(4112..4981)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889041.1"
                     /locus_tag="C8250_RS23175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_023190"
                     /product="tyrosinase family protein"
                     /protein_id="WP_138351289.1"
                     /transl_table=11
                     /translation="MYTRQNQKNMTSAQRAKFVRAVLKLKSKGIYDDFVRIHGQYYVSD
                     GDTGLRVGHMTPSFFPWHRRLILEFERKLQSVDSSVTLPYWDWTRDNAKTSSIWASDFL
                     GGDGRRSDGQVMTGPFAYVNGNWPITVAVTDEKYLTRQFGRPDRPIGLPTKADLDKAIA
                     ESVYDTSPWNSTATTKGFRNRIEGWTVSETQGGLLHNRVHQWVGGHMTGATSPNDPVFW
                     LHHAFIDLIWLRWQRKYPKSGYLPARPLAASDPQHGRVVSLNEPMPPWNTKPSEVMNLQ
                     NIYRYEEN"
     gene            complement(5001..5486)
                     /locus_tag="C8250_RS23180"
                     /old_locus_tag="C8250_023195"
     CDS             complement(5001..5486)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129287.1"
                     /locus_tag="C8250_RS23180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_023195"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_138351290.1"
                     /sec_met_domain="melC (E-value: 1.6e-40, bitscore: 137.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEAKENGGGKASPSAHTRRRVLRAAFTAAVAVTGTTAVLHPIIT
                     AGESDAGKTGSGRSDAGSDRAGSAPAAAAGAFDEVYRGRRIQGVPTGTADAATTEGSPV
                     VILIDGRQLHVMRRADGTYLSVANHYQPYATPLDTARGAVDVIGGAKLSLAGGAYHH"
     gene            6108..>7064
                     /locus_tag="C8250_RS43655"
                     /pseudo=""
     CDS             6108..>7064
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006346978.1"
                     /locus_tag="C8250_RS43655"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="glycosyl hydrolase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MGAFTSSGADGVQRIADLQQWLGGTHLTVGHTYLPGESWDNIEGE
                     PELLQPWAQWRKAEPDRLFVLNVPMQERNEQHISDSQVRSLLRSGAAGRFDSHFTELAR
                     RLVALGVPDTVVVLGWEMNGTTYTHRCGPDPESWKTYWNRIVTAMRAVPGQRFRFDFTP
                     NRGQDAIGWTSCYPGDSTVDIIGMDSYDQPPGDSFYDQVEEPFGLQAQVDFAAAHNKAI
                     SYPEWGLFRNGDNADYMRMMLQWINDHRPVYQTLTDYCPHGVWQCTDNPKSSKVFRSTN
                     FARVEPTPTPVPTPVPTPTTTPAPTPSTTPTPSGTPTP"
     misc_feature    complement(6114..6125)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 20.58"
                     /tool="antismash"
     gene            8010..8420
                     /locus_tag="C8250_RS23190"
                     /old_locus_tag="C8250_023205"
     CDS             8010..8420
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049140.1"
                     /locus_tag="C8250_RS23190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_023205"
                     /product="rodlin"
                     /protein_id="WP_138351292.1"
                     /transl_table=11
                     /translation="MLKKFMASAAVAVSIVGLSAMAAPQALAIGNDEGASTTNGNGAEQ
                     SYGNTHTDGVMSPQVGLVQGTLNKPCVGLPLKANIGSLVGLVPITAQDINVLSSPQNQQ
                     CTENSSQIKGDEPLSHILSQIPVLSGNGVGNG"
     misc_feature    complement(8674..8689)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.36"
                     /tool="antismash"
     misc_feature    8702..8716
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.93"
                     /tool="antismash"
     misc_feature    complement(8704..8715)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.89"
                     /tool="antismash"
     gene            8716..9498
                     /locus_tag="C8250_RS43660"
     CDS             8716..9498
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015660307.1"
                     /locus_tag="C8250_RS43660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_244809304.1"
                     /transl_table=11
                     /translation="MRTHRTTGVIVSTALVGALAFGTAGTAFASPDVAPTGTFTGRTAP
                     AAPDPAALLAQVTALGNVGAVLTPVTDLVKAVLAAPGNKLSAEDATKHADAVKAAIAAA
                     KVPAAPAVPAAPAAPAVPAAPAAPAVPAVPAVPAVPAVPALPATPALPGVPALSGVSMH
                     GVSAADRSAPRAADVAGDALAGLQTAVDNLLKAATSGDVAGVLTSALATVTGLVNFLVA
                     TVLGGALPAPDLPGLPKLPALPTLPVPLPPLPLNDLPG"
     misc_feature    10238..10252
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.19"
                     /tool="antismash"
ORIGIN
        1 ttccggccat caccccgtgg gacggggacg agccgcagtt cgcgctgacg gactacggcc
       61 gcagcgccga ctgcgcccgc atgcggcggt gttcggtctg cgacacgctg atgccaccag
      121 gaccggtgtg gcgtgtggtg ggtgccgcgg agagcgccgc gatcgccgac gcgctggccg
      181 ccgggcgccc gtaccgcaat ctcgccccca ccctggaggg cccggggcac cgcgcctgca
      241 tgctctacgc ctcgatggtc tgcccctacc tggcccgtcc gaacgcgcgc cgcgggttgt
      301 cggcacagcg gccggacgag ctgaccggac atgtcgtccg gggcgcggta cggggtgcga
      361 cgggcgcggt ggtgggcttc ggcgactacg agttcgccgt caccggcacc caggtgctgt
      421 tccgcttcct cgacatcgtc gagttcctgc cgcacgacac gtccgacgcg cacctcgaag
      481 agctgcgcgc cgaactcgcc gcccgggacc ggcccggagc acccggggat cagccgcggt
      541 aggacggcag cccggtgtag gtggacacca gcttcggcgc ggcggagctc ttcgtggaga
      601 gcgtgacgat gccggccggc gtcctggcgg cgatggtgct gccgtccggg gcccaggcgg
      661 gctcggtgta gtccgtggtg gcgtacggcg tcaggtcctt gaagaccggg ccggcgtccg
      721 tcgggttctc caggaacagg tggtcgtgac cgccgaccga gcggacgaag acgacctcct
      781 cgtgctcgga gtgcgacatc gccggctgcg agccgtgcgt gagcgcggat ccctgctggc
      841 ggacgtagtc gtcacggatg aagacctcgc cggtgccgga gttcgcgtac acggcgctgc
      901 cgtgggtgcc gcccgcgctc ggccagacgt tgccggtctg cgggacgggc ttcacctcgt
      961 cgccggcctc ccagcccaga ccgagcttct tcggcgtccc gttgacggcg ctggagggga
     1021 cgctgtccag ctcggagacg ccgctcttcc ggaccgcgaa gaggatgttg ctccgcccgg
     1081 acagctccgg aatcgagtcg tacgccgtca cctgccacgt cgggtgcgac cagttctgcc
     1141 cgcccgggtt ccgggcgacg gtgacgcgtc cggtgccgtt cgggttggcg agggcgaggt
     1201 tgccggcgcc gtcgatgaac gcggccttgc ggccgtccgg cgaccaggcc aggtccctga
     1261 cggcggtgcc gaagtccacc ctggtgccgt tcatgaccac gtaccgggtg ccgtcgctga
     1321 tggtcagttt gctgttcgcc gtgccgttca ggaccggcgc gcgcgtgctg cccgccgtgg
     1381 tgggcgtgcc cgtgggggtg gagccgccgg ccggagtggt ggaggcactg gcgctcggag
     1441 cggtgccggc cgacggggtc gcaccgccgg ccggagcggc ggaactgcca ctgctcgggg
     1501 tgttggtggc ggccggcgga gtggtgaccg cccgcgcgcc cgacgtcggg ccgcagccgg
     1561 tgagcagtgc cgcggtggcg gcggtgaggg tggcggtgag agcgacggcg acggcggtgc
     1621 tgccacgggt acgagcggac atgagcggtt ccccccaggg acgttgatag gagaagagtg
     1681 gcgattcaac gtccgtgacg tgtgtgaccg gtgtcacgga cgtgcaacag cgattccggg
     1741 attcatgggt gtgggaccga tggacccggg taggcgggtg ccattgccgg ttgctcctgg
     1801 gaggatgagg aacatgacag cgctagtgaa ctcggacgac acagctgtgg tcgatgtcca
     1861 ggccacgggc ggctgtgcct cgtcgatgac ggaactgccg tggatcgagg aggcgggcaa
     1921 ggtcgcgccg aaggatgcgc gggccctgtc gaagctcttc ttcgaccgcc tgcagagcct
     1981 ggaggagggg acgcgcgacc accagtacgc gcggaacacc ctcatcgagc tgaacctgtc
     2041 cctggtccgg ttcgccgccg gccggttccg caaccggtcg gaggacatgg aggacatcgt
     2101 ccaggtcggg acgatcggcc tgatcaaggc gatcgaccgg ttcgacctgt cgcgcgaggt
     2161 cgagttcacc acgttcgcca ttccgtacat caccggcgag atcaagcgct tcttccggga
     2221 caccagctgg gccgtccatg taccgcgcag cctccaggag cgccggatcg cgctggcgca
     2281 ggccaaggac gcgctctccc agcggatgga ccgctccccg accaccgcgg agctggccga
     2341 atacctggac ctgaccccgg ccgaggtgaa cgaggggctg gtggccagca acggctacac
     2401 cgccggctcc ctggacctcc ccgcggggga gaacaacgag gaggccgcgg gcagttcgtt
     2461 cgtcgaccgg ctcggcgagg acgacccggg catggaggcg gtggagaacc tgcaggctct
     2521 caagccgctg atggcggaac tcggcgagcg ggaccgcgcg atcctgcgga tgcgcttcgg
     2581 ccaggagatg acgcagtccg agatcggcgc cgagctcggg gtctcccaga tgcatgtgtc
     2641 ccggctgctg acccggacgc tcacccggct gcgtaccggg ctgctcaccc aggcgtaaca
     2701 cgtcggaacg tcagcacgaa ggtccccgcc ggcactgtcc ggcggggacc ttcgcgctgt
     2761 gacggccggg gcggtcagcc ctggagggag ccgatcggga cgaagcagtg cgcggacccg
     2821 gccagcatgg tctcgtcgcc cacgaaggac ttgagcacct cttcgctgac cggcttcccc
     2881 ggtgccgcct ccggccacgg ccgggtggcg aacatgaaat acaccatgcc gcggtcgcgg
     2941 gccgcgtcca cccactgctc gggaacgggg caggcagcgt gcaggtacgg catggtcagg
     3001 acggcctggc cgccctcgac gaggagcttc acgggggcct tgggcacctg ggcgatgtcc
     3061 aggatggtcc cgccgatcgg caggccgacc tcctcgatcg ccgcggtgat ggccgcctcc
     3121 gcggcctcgg ggccgccctc gacatcgccg agggagtagg ccaggaggaa cgcgatgtcc
     3181 cgttcgtcct cgatgtgctc gccggcccag gggatcaccg cgagggtgcc catctggctc
     3241 tgccgggtga tgtccggagc ggtgctggtc tgactcatgg caacggactg taacggcggg
     3301 ccgcgcccga ccgggcccgc atgtcacctg agagagcgag tcctgaccgc taaacccacc
     3361 gaacgaggtc gtgtcgatcg cacatgcgat acgacggtat gtccgtggaa ggggtgcgat
     3421 gcaccgcccc cggccgtgac cggtggggcc cgtggtcgtt ggacagggcg catcaccccc
     3481 gcgcgaaggc tccggagcgt ccaagcttcg gagcctttgg tgtcggggcc gtcctcaccg
     3541 gcggtgacgt tcaccagttc ggctcaacct gcgtgactat gcggcataca tacgcgttgc
     3601 gcagggcgtc aatgccgcag gtcctgcggt ttgggtttca ccatcaagga gatttcgatg
     3661 tcgcgtattg ccaaggcagc cgccatcacc gccgcggccg gtgccgttct ggctgcgggc
     3721 gccggcatgg ctgccgccga cgccggtgcc cagggtgctg cggtcggttc ccccggcgtt
     3781 ctgtccggca accaggccca gatccccgtt cacgtgcccg tcaacgtctg cggtaactcc
     3841 gtcaacgtga tcggtctcct gaacccggcg ttcggcaacc actgcgagaa cgtctcgggc
     3901 ggctggtagt cccggttctc aacagttgta gggccactgg accggacgac gaccggcccg
     3961 gcacagcggg ccggcgggac gcagaccgac cgtcccgtca cctgtccgtc cggcggcccg
     4021 gggcgaacac ccccatccca ctggtgcggc cccccgcgat gccttcgcgg ggggccgcac
     4081 tcgtgcgttc gcccgccggt ccgggtgcgg gtcagttctc ctcgtaccgg tagatgttct
     4141 gcaggttcat gacctcggac ggcttcgtgt tccacggcgg catgggctcg ttcagactga
     4201 cgacgcggcc gtgctgcggg tcggaggcgg ccagcggccg ggccggcaga tagcccgact
     4261 tcgggtactt gcgctgccag cgcagccaga tgaggtcgat gaaggcgtgg tgcagccaga
     4321 agaccgggtc gttgggcgag gtggcgcccg tcatatggcc gccgacccac tggtggaccc
     4381 ggttgtggag gagtccgccc tgcgtctcgc tgaccgtcca cccctcgatc ctgttgcgga
     4441 agcccttggt ggtggcggtg gagttccacg gggaggtgtc gtagaccgat tccgcgatcg
     4501 ccttgtcgag gtccgccttg gtgggcagcc cgatcggacg gtccgggcgg ccgaactgcc
     4561 gggtcagata cttctcgtcc gtgacggcga cggtgatcgg ccagttgccg ttgacgtagg
     4621 cgaaggggcc ggtcatgacc tggccgtcgg accgccggcc gtcaccgccc aggaagtcgg
     4681 acgcccagat cgaggaggtc ttcgcgttgt cccgcgtcca gtcccagtag ggcagggtga
     4741 cgctggagtc cacggactgc agcttgcgct cgaactccag gatcagccgg cggtgccacg
     4801 ggaagaacga gggcgtcatg tggccgaccc ggagcccggt gtcgccgtcg gagacgtagt
     4861 actgaccgtg tatgcggacg aagtcgtcgt agatgccctt gctcttgagt ttcaggaccg
     4921 cgcggacgaa cttcgcccgc tgggcgctgg tcatgttctt ctggttctgg cgggtgtaca
     4981 ccgtgtcggc ccttctgcgg tcagtggtgg taggcgccgc cggcgagcga cagcttggct
     5041 ccgccgatca cgtcgaccgc gccgcgggcc gtgtcgagcg gggtggcgta cggctggtag
     5101 tggttggcca cactcaggta ggtgccgtcc gcgcgccgca tgacatggag ctgccggccg
     5161 tcgatgagga tgacgacagg ggacccctcg gtcgtggcgg cgtcggccgt gccggtgggc
     5221 acaccctgga tgcggcggcc ccggtagacc tcgtcgaagg ctccggcggc cgcagccggg
     5281 gcggaccccg cccggtcgct gccggcgtcg gaccggccgg acccggtctt ccccgcgtcg
     5341 gactccccgg ccgtgatgat cgggtgcagt accgccgtcg tgccggtgac ggcgacggct
     5401 gcggtgaacg ccgcccggag cacccggcgg cgcgtgtggg cggacggtga cgctttcccg
     5461 ccgccgtttt ccttggcttc gctcatgctc agctctcctc gatatcggcc gacagggccg
     5521 gacctgtgag gagcggcggt atcgcgccgc attcgcccgg acgggcggcg ccgactgtcc
     5581 gtcagctatg caccatcatc ctgctcccgg cgtgaggcgc tcgctcatcc cggcagccgg
     5641 agtacagaac tccattcgtg cgggtggcgt ttgcctccga cccgtgacga acgtaaatcc
     5701 gatatacggc ggcggaagtc aaccagggac ccggtattca cccgatctga cgatccgacg
     5761 gattcaccgg cggttctgac cggcgcacgt ttcctaatgg actatcagaa gaggcccgct
     5821 cgcccggctg ctcatacttt ctgaattacc gtccggagaa agtgctccgt gtcggtaacg
     5881 tcgcaggagg agcaaacatg gcccaccgac gccagaggct gactggtgca tgtgtcggag
     5941 cggccaccgc cggcctgctc atgctcggga tgacccacac cgccgtcgac gacatgcgcg
     6001 tcgcggcgga cgccacggat ccggcaccgc cgtcaccggc cgccggaccg ccgggtcccg
     6061 ggccggccgg ggccctgcgc cccgcgtcga ccgccggata cacgccgatg ggggcgttca
     6121 cgtcctccgg tgccgacggt gtgcagcgga tcgccgatct gcagcagtgg ctgggcggta
     6181 cccatctcac ggtgggtcac acctatctcc ccggcgagag ctgggacaac atcgagggcg
     6241 agcccgagct gctgcagccg tgggcgcagt ggaggaaggc ggagccggac cggctcttcg
     6301 tcctcaacgt gccgatgcag gagcgcaacg agcagcacat ctccgacagc caggtgcggt
     6361 cgctgctgcg ctccggcgcg gcgggccggt tcgacagcca cttcacggag ctggcgcggc
     6421 gactggtcgc gctgggtgtg cccgacacgg tcgtcgtgct gggctgggag atgaacggca
     6481 ccacgtacac ccaccgctgc ggtccggatc cggagtcctg gaagacgtac tggaaccgca
     6541 tcgtgacggc gatgcgcgcc gttcccggcc agcggttccg gttcgacttc acgccgaacc
     6601 gcggccagga cgccatcggc tggacgtcgt gctacccggg tgactccacc gtggacatca
     6661 tcggcatgga ctcctacgac cagccgccgg gcgactcctt ctacgaccag gtcgaggagc
     6721 cgttcggtct ccaggcgcag gtggacttcg cggccgcgca caacaaggcg atctcctatc
     6781 cggagtgggg actcttccgc aacggcgaca acgcggacta catgcggatg atgctgcagt
     6841 ggatcaacga tcaccggccg gtctaccaga cgctcaccga ctactgtccg cacggcgtgt
     6901 ggcagtgcac ggacaacccc aagtcgtcga aggtcttccg gtcgacgaac ttcgcccgcg
     6961 tcgagccgac gccgacgccg gtaccgaccc ctgttccgac gccgacgaca acgcctgcac
     7021 ccacgccgtc gaccacgcct acgccgagcg ggacgccgac gccgggcggg acaacgagtg
     7081 tgccgctgac gtccacgccc gcggacgtgc cgtcgccgtc gccgacggac ggcgcgaccc
     7141 cggcggagtc cccgaccgtt ccggtgagcc ccgcgccggg tgacgccacg acgccacccg
     7201 cgacggtgac gccgcccgcg accgtgaccc cgaccgtcac cccggccgcg actccggatc
     7261 cggccgtggc ggagaccccg tccgccgcac cggttccggc cgacgcgacg gcgccggtgg
     7321 tcgctccggc ggccccgccg accgccgatc cggtggtggc gccggagccc gcgcccgggc
     7381 tgagcccggc gcccgcgccg agtcccgccg cgacgctcac ccccctgccg gctccgggtc
     7441 ccgatccggc cgccaccggc gccacggcac ccgcgcctgc tccgaccgtc atggttccgg
     7501 tgccggtgac cctcccgacg atcggtgcgc cctgcacacc gacgccggat cccggcttca
     7561 gccccgcccc cagcctgtcg cccgcaccgg ttccgacgtc tcctgttccg acgtctcccg
     7621 ctcctgcgcc gactgcgtcg ccgacgtcgc ccgcgacgcc ggggccggtc gtccaggtgg
     7681 ccggacccgt gtgcacggcg gttccggtgg ggcccttcaa gcccgctccg acgctcagtt
     7741 cacccccggc gcccgccgag gtgtgcctgc cactcgacat gggagacgaa ttcaagcgcc
     7801 agttcggcac cggaaagctg tgcttccgcg tcgaatggca gacccccaag aagagctgac
     7861 cgtaacgcga cgcgcccggc ggcctgccgc cgggcgcacg catggaaaat cggttgggct
     7921 agtggagtga aatcgcggaa ccaaaccgga gatgggcagt tgatcagttc gcttcaatga
     7981 caggaacata gtttcaagaa gggcgaaccg tgctgaagaa gttcatggcc agtgcagcag
     8041 ttgccgtttc catcgtcggt ctctccgcca tggctgcccc gcaggcgctg gccatcggta
     8101 acgacgaggg agcctccacc acgaacggta acggggcgga gcagagctac ggcaacaccc
     8161 acaccgacgg tgtgatgagc ccccaggtcg gcctcgtcca gggcacgctc aacaagccgt
     8221 gtgtcggcct gccgctcaag gcgaacatcg gctcgctcgt cggcctcgtg ccgatcacgg
     8281 cccaggacat caacgttctg tcgtcgccgc agaaccagca gtgcacggag aactcctcgc
     8341 agatcaaggg cgacgagccg ctgtcgcaca tcctgagcca gatcccggtg ctctcgggca
     8401 acggcgtcgg caacggctga cagctgacac ctcatcgcat ggcggtggcc gcgtactccg
     8461 tacgcggcca ccgtcgcgtt ctccggcgct tccgattttc ccggggcacc cggttggctc
     8521 acgtggccgc cgacaatccc ttcgcggcgg ccccggacgc cgaatgcact gtgtccaggg
     8581 gctgttcacg gccggtcccg gccggagggg gagcgcgaca cgccgggtgc cggtagttga
     8641 caggttgcgg ggccgccgtt cgctttcgac ggttgtggag tacacccgtc tgttcagcga
     8701 agggaaaaca tcttcatgcg cacccaccgg acgaccggcg tgatcgtctc caccgccctc
     8761 gtcggcgcac tcgcattcgg cactgccgga acggcattcg cgtcccccga cgtcgctccc
     8821 accggtacct tcaccggtcg caccgccccg gcggctcctg atcccgccgc actactcgcc
     8881 caggtcacag ccctcggcaa tgtcggcgcc gtcctgacgc cggtgacgga cctggtcaag
     8941 gccgtgctcg cggcccccgg caacaagctg tcggcggagg acgccacgaa gcacgccgac
     9001 gcggtcaagg ccgcgatcgc cgcggccaag gtccccgcgg cgccggctgt cccggccgcg
     9061 cccgcagcgc ccgccgtccc cgccgctccg gccgccccgg ccgttcctgc ggttcccgcc
     9121 gtgccggccg tgccggccgt ccccgcgctg ccggccaccc ccgcgctgcc cggagttccg
     9181 gcgctgtccg gtgtgtcgat gcacggcgtc agtgctgcgg accgctccgc accgcgtgcg
     9241 gccgacgtcg cgggtgacgc gctggccggt ctgcagacgg ccgtcgacaa cctgctgaag
     9301 gcggccacgt cgggtgacgt ggcgggtgtc ctgaccagcg cgctcgcgac cgtcaccggc
     9361 ctggtcaact tcctggtcgc cacggtcctc ggcggtgcgc tgccggcgcc ggacctgccc
     9421 ggcctgccga agctcccggc gctgccgacc ctcccggtcc cgctcccgcc gctgccgctc
     9481 aacgacctcc ccggctgacg cgttcaggac cccgacgacc tgcggcacgc ggatggacgc
     9541 gcgccgcagg tcgtggtctt ttccggggcg ggtgccggcg gaagggcgcc tcagacccgc
     9601 ttcgcccgcc aggtgttcag ccgcgaccgc cagctgcgca ccaccggcag atagcggcgc
     9661 accgccgcgg ccgcccggtc ccggagtgcg gcccgtgcga cctggagccg gagggcgggc
     9721 gcgaacagcg gcgacgtcat cagcagccgc tggttggcca cgaggtcggg gcaccagggc
     9781 agcttgttcg cctcgttgcc gcgcagcagg ctcagcgctc cgccgccgcc cgatgcggtg
     9841 gcgtgaccgg cgccgttcct gatcagcatg gtggtgagat cggccttggt ggcgcgcagc
     9901 cgggggtcgg cgccgaagag atagccgccg accagttgcg gggagagcat cttgaggtcc
     9961 gccgccagca cttcgccgtc cagccggtac tcggtgatga cggcgtcgcc gccggccacc
    10021 atgtgctgcc cggcccgctt gaggtgcgcg gcgaagcgcg gcctggcgtg ctccggggtc
    10081 accccgcgtc cctcccactg caggatgtgg aggcgcagca ggttctcgac ggccgtgggc
    10141 acctcgtgct cggggacgac gcgctcctcg atgcccaggg cgtcgagttt gcgtacgtag
    10201 gcccgggtgc gctggccgcg ggaggtggac atccgggtga tcagctcggc cagcgggaca
    10261 ccgggcagct ccatgcagac ggagtccgcc agccgccgct tcggtccctg ccagcactcg
    10321 tggagccgtt cggcggcggc gcccggccgt acctcgcgca ggtcgatcag cgcgccgcgg
    10381 gccgcgcggc gcagtccggt ggccatcgcg gtgacggcgg cgtcggtggc gttgtcgtcg
    10441 agcagcacgt cggagaagtc ggtgatgctg ccgcccagcg gcacca
//
