LOCUS       NZ_CP048835            10453 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. So13.3 chromosome, complete genome.
ACCESSION   NZ_CP048835
VERSION     NZ_CP048835.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. So13.3
  ORGANISM  Streptomyces sp. So13.3
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Quezada-Solis,D., Khalil,Z.G., Capon,R.J.,
            Andreote,F.D. and Barrientos,L.
  TITLE     Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed
            Culture and Elicitation Conditions for the Production of
            Antimicrobial Compounds
  JOURNAL   Biomolecules 10 (5), 673 (2020)
   PUBMED   32349314
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Lamilla,C., Abanto,M., Maruyama,F., Jorquera,M.A.,
            Santos,A., Martinez-Urtaza,J. and Barrientos,L.
  TITLE     Antarctic Streptomyces fildesensis So13.3 strain as a promising
            source for antimicrobials discovery
  JOURNAL   Sci Rep 9 (1), 7488 (2019)
   PUBMED   31097761
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K. and Barrientos,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Centro de Excelencia en Investigacion
            Biotecnologica Aplicada al Medio Ambiente (CIBAMA), Universidad de
            La Frontera, Av. Francisco Salazar 01145, Temuco, La Araucania
            4811230, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2020
            Assembly Method                   :: Unicycler v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140.0x
            Sequencing Technology :: Illumina MiSeq; Illumina HiSeq; Oxford
            Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005860785.2-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:03:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,707
            CDSs (total)                      :: 8,616
            Genes (coding)                    :: 8,274
            CDSs (with protein)               :: 8,274
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 109 of 342
            Pseudo Genes (incomplete)         :: 268 of 342
            Pseudo Genes (internal stop)      :: 24 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:45:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2924963
            Orig. end                         :: 2935416
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Laboratorio de Biologia
            Molecular Aplicada, Universidad de La Frontera, Avenida Alemania
            0458, Temuco, Chile.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10453
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="11"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10453
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10453
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5453](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5453
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    381..394
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.58"
                     /tool="antismash"
     gene            complement(392..1483)
                     /locus_tag="C8250_RS13290"
                     /old_locus_tag="C8250_013330"
     CDS             complement(392..1483)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960017.1"
                     /locus_tag="C8250_RS13290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_013330"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_138355044.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 6.3e-20, bitscore:
                     70.6, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDRGALHVKGRVLVGPEDVRDEVWVLDGRISYDRPAGPLEVTTV
                     EGWALPGLVDAHCHVGLDTHGPVDAETAEKQALTDREAGTLLLRDAGSPSDTRWVDDRE
                     DLPRIIRAGRHIARTRRYIRNYAHEIEPGDLTAYVRQEARRGDGWVKLVGDWIDREAGD
                     LTACWPRGALEEAIAAAHEEGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEDTIPLF
                     VERGVAIVPTLVNIATFPSMAAGGEAKFPRWAAHMRRLHERRHATVGAAHDAGIRIFAG
                     TDAGGSLAHGLIADEIAELVTAGLSPVEALSATTWGARAWLGRDGLTEGASADLIVYAA
                     DPRADVRVLADPRRIVLRGRVVG"
     misc_feature    1483..1497
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.9"
                     /tool="antismash"
     gene            1597..2640
                     /locus_tag="C8250_RS13295"
                     /old_locus_tag="C8250_013335"
     CDS             1597..2640
                     /NRPS_PKS="Domain: Aminotran_5 (20-291). E-value: 9e-29.
                     Score: 92.4. Matches aSDomain:
                     nrpspksdomains_C8250_RS13295_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957694.1"
                     /locus_tag="C8250_RS13295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_013335"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_138355023.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.8e-22, bitscore:
                     77.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPLLDLPPLTAARFAGIERRVAALLGTEQDVVITQGEALLPLE
                     GCIRGGARPGSAALNVVTGPYGKTFGDWLRDAGAEVTDLAVPFHGAVSAEQVERALAEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFMLDAVASVAAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERMAANPAAPRRSYLSLLDWKERWIDTGRAALPHAP
                     AQLEMLALEACLERIDADGLDTVIARHRAAATATREGALALGGGLEPYVYEARDAAPVA
                     TTLRVPAGTDATALVARALALDPSVPLVAGGGALAKEMVRVNHYGPDADPAVVARSLAA
                     LGTALSE"
     aSDomain        1657..2469
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C8250_RS13295_Aminotran_5.1"
                     /evalue="9.00E-29"
                     /label="C8250_RS13295_Aminotran_5.1"
                     /locus_tag="C8250_RS13295"
                     /protein_end="291"
                     /protein_start="20"
                     /score="92.4"
                     /tool="antismash"
                     /translation="RRVAALLGTEQDVVITQGEALLPLEGCIRGGARPGSAALNVVTGP
                     YGKTFGDWLRDAGAEVTDLAVPFHGAVSAEQVERALAEHPEIDFVSLVHAEAATGNTNP
                     VAEIGEVVRAHGALFMLDAVASVAAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAVSVSA
                     RAWERMAANPAAPRRSYLSLLDWKERWIDTGRAALPHAPAQLEMLALEACLERIDADGL
                     DTVIARHRAAATATREGALALGGGLEPYVYEARDAAPVATTLRVPAGTD"
     misc_feature    2829..2844
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3000..3515
                     /gene="ectA"
                     /locus_tag="C8250_RS13300"
                     /old_locus_tag="C8250_013340"
     CDS             3000..3515
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560637.1"
                     /locus_tag="C8250_RS13300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_013340"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_138355024.1"
                     /transl_table=11
                     /translation="MNAATEWKAPLAPAFLDTPTVDDGGAMWRIARDSRTLDLNSSYSY
                     LLWCRDFAGTSVVARDADGAPVGYVTGYLRPEEPRTLVVWQVAVDAAQRGRGVAAAMLD
                     HLAARVAVDDVRWIETTITPDNAASQRLFTSFAERHDVPVEREVLFPGALFPDGHDSEV
                     LFRIGPTV"
     gene            3638..4918
                     /gene="ectB"
                     /locus_tag="C8250_RS13305"
                     /old_locus_tag="C8250_013345"
     CDS             3638..4918
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-364). E-value: 1.5e-73.
                     Score: 239.6. Matches aSDomain:
                     nrpspksdomains_C8250_RS13305_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006375098.1"
                     /locus_tag="C8250_RS13305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_013345"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_138355025.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.4e-75, bitscore:
                     250.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITAPDMTVFESLESEVRSYCRSWPVIFDRAQGSVMYDEDGHAY
                     LDFFSGAGALNYGHNNPVLKRALLDYLERDGVTHGLDMSTTAKRTFLERFQQVVLEPRG
                     LDYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYFDGRVPDFLWFERLLEDQGSGLNQPAAVIVETIQGEGGINVARP
                     EWLRALADLCKRRDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSISGYGMPMS
                     LALFKPELDVWEPGEHNGTFRGNNPAFVTATAALDTYWADGQLEKQTVMRGEQVETALR
                     EICTAQARAGAEYRGRGLAWGLQFIDPSIAGRVCARAFELGMLLETSGPRSEVVKLLPA
                     LTITPDELDEGLRTLTRAVSDVVGESA"
     aSDomain        3725..4729
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_C8250_RS13305_Aminotran_3.1"
                     /evalue="1.50E-73"
                     /label="C8250_RS13305_Aminotran_3.1"
                     /locus_tag="C8250_RS13305"
                     /protein_end="364"
                     /protein_start="29"
                     /score="239.6"
                     /tool="antismash"
                     /translation="DRAQGSVMYDEDGHAYLDFFSGAGALNYGHNNPVLKRALLDYLER
                     DGVTHGLDMSTTAKRTFLERFQQVVLEPRGLDYKVMFPGPTGTNAVEAALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNSMKRAGAGIPLVHGTPMPFDNYFDGRVPDFLWFERL
                     LEDQGSGLNQPAAVIVETIQGEGGINVARPEWLRALADLCKRRDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTLSKSISGYGMPMSLALFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATAALDTYWADGQLEKQTVMRGEQVETALREICTAQARAGAEYRGRGLAWGLQF"
     gene            5001..5453
                     /locus_tag="C8250_RS13310"
                     /old_locus_tag="C8250_013350"
     CDS             5001..5453
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="C8250_RS13310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_013350"
                     /product="ectoine synthase"
                     /protein_id="WP_138355026.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.7e-52, bitscore:
                     174.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRDVTAETGTWRSKRIVLAAEGVGFSMHETTMY
                     AGTVTSMWYANHIEAVLCVDGEGELTNDETGETHWISPGTLYLLDGHERHTMRPKTDLR
                     FVCVFNPPVTGREVHDENGVYPLLTETGGTTATRTRTRNGTETEGA"
     gene            5458..6357
                     /gene="thpD"
                     /locus_tag="C8250_RS13315"
                     /old_locus_tag="C8250_013355"
     CDS             5458..6357
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="C8250_RS13315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_013355"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_138355027.1"
                     /sec_met_domain="PhyH (E-value: 2.7e-37, bitscore: 128.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTDTITSRYPTRGASEVTTPRQDPVVWSEYGTPGPIQPAELR
                     GYDRDGFLTVDQLLLPGEVGRYRDELDRLCADPAIRADPRAIVEPKSGSIRSVFEVHRI
                     SEVFAELVRDPRLADRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSVALTENHDTNGGLMIMPGSHRTFLGCSGETPKDNYKRSLQMQDAGTPSDEA
                     LTELAGEHGIKLFTGAAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENAAVEPFA
                     APVRRPEFIGARDFTPVR"
     gene            complement(6415..7026)
                     /locus_tag="C8250_RS13320"
                     /old_locus_tag="C8250_013360"
     CDS             complement(6415..7026)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671939.1"
                     /locus_tag="C8250_RS13320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_013360"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_138355028.1"
                     /transl_table=11
                     /translation="MELKELPFGLRAYGPAADPSYQDGALVLTAGPGQDRFVHPGRAPK
                     VPAADAPRLLGTPPEGDFQLIARVTARLRAAGDAGALYLQVADEQWAKLCLELSPARYT
                     VVSVVTQGSSDDANSWEVDGDHVWLRITRTGRAFAFHASADGERWTFVRLFALGTEREA
                     AAASVGFMTQCPSPDADGCTAVFDRITFRSGAPADLRDGG"
     gene            complement(7044..8576)
                     /locus_tag="C8250_RS13325"
                     /old_locus_tag="C8250_013365"
     CDS             complement(7044..8576)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004607.1"
                     /locus_tag="C8250_RS13325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_013365"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_138355029.1"
                     /sec_met_domain="Aldedh (E-value: 5.1e-39, bitscore: 133.0,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVPVWSVDPRTGKQREQVADEATTAEIDAAVRAAAAAAPALADR
                     SVRALLLRTAADLLEEAGPQVLEAADAETALGPGRLTGELARTTFQLRAFADIVDEGAF
                     LDVVIDHADPAAKPAPRPELRRYKIPLGVVAVYSASNFPLAFSVPGGDTASALAAGCPV
                     VVKAHPDHPATSELCASVLRRAAVRVGLPADVVAVVHGFQAGIDLIKHPLVSAAGFTGS
                     VRGGRALFDAAAARPRPIPFHGELGSLNPVVITEAAADERAEQIGAGLAGSMTLGVGQF
                     CVKPGLVLAPAGAAGDRLLKAVTAAVSDSDAGVLLDVRMRDAFVAGVEARAALPGVEAP
                     VTPGSGGEHTVSAGYLTVPADRLAAEGPHDLLLEECFGPVTVVARYEDRAQITAVLERL
                     PGNLTATVHIGEEEAGQEDGPATALLAELTPLAGRLLVNGWPTGVAVAPAQHHGGPYPA
                     TTSTSTSVGGTAIERWLRPVAYQDTPVALLPAELRDENPLHLPRRFDGRLEI"
     misc_feature    8628..8642
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.93"
                     /tool="antismash"
     gene            8712..9482
                     /locus_tag="C8250_RS13330"
                     /old_locus_tag="C8250_013370"
     CDS             8712..9482
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="C8250_RS13330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_013370"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_138355030.1"
                     /transl_table=11
                     /translation="MSAGDGGGSQVKSAVRTVELLEFFAGRPGMHSLAAVQESVGYPKS
                     SLYMLLRTLVELGWIETDATGTRYGIGVRALLVGTSYIDGDEVVAAARPTLDRLSDDTS
                     ETIHLARLDGTSVVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATYTDEQVRKLL
                     PETLEPLTEHTHTDREKLIEELAVIREQGFAVDREENTLGLRCFGIAIPYRTPARDAVS
                     CSVPVARLTPAHEQMIKDTLFDARDRLTLATRRL"
ORIGIN
        1 gcctgggccg ccggggcgac ggccagcgca cctgccgcaa gcgcgacggc cgcgccgacg
       61 gcggcggccg aacggcgtgc gggcaggctg aagttggagc tgtacacggt ggaaccccca
      121 cgagtggctt ggagtcagcg gcgccgcttg aggggacaac gcgaccgccg gtgacctcgg
      181 tcccgccata tttacgcact gagagtgaac tggcgactac ctgaagcgag ttcacccata
      241 cgggtggttt ccggcattat cttcgaatct tgctgcacgc ccgttcccac ccgtcacccg
      301 tgccacgtga tgacggcccc cacctccgtt gacgcgccga ggagtacgcg gtggtgctca
      361 acgacccgca cctgcttcgc gtaacgctgc gtcaacccac cacccgcccg cgcagcacga
      421 tgcgccgcgg atcggccagc acccgtacgt ccgcccgcgg atcggcggcg tacacgatga
      481 ggtcggcgga ggcgccctcg gtgagcccgt cgcggccgag ccatgcgcgg gcgccccagg
      541 tcgtggcgga cagcgcctcg accggcgaca ggcccgccgt gacgagttcg gcgatctcgt
      601 cggcgatcag gccgtgggcc agcgagccgc ccgcgtcggt gccggcgaag atccggatgc
      661 cggcgtcgtg cgcggcgccc accgtggcgt gccggcgctc gtgcagccgg cgcatatggg
      721 ccgcccagcg cgggaacttg gcctcgccgc cggccgccat cgacgggaac gtcgcgatgt
      781 tgaccagggt cgggacgatg gcgacgccgc gttccacgaa gagcgggatg gtgtcctcgg
      841 tcagcccggt ggcgtgctcg acgcagtcga tacccgcctc cacgaggtca cgcagcgagt
      901 cctcggcgaa gcagtgcgcg gtgacccgcg cgccctcctc gtgggcggcg gcgatggcct
      961 cctccagggc gccgcgcggc caacaggccg tgaggtcacc ggcctcgcgg tcgatccagt
     1021 cgccgacgag cttcacccag ccgtccccgc gccgcgcctc ctggcggacg tacgccgtca
     1081 gatcccccgg ctcgatctcg tgcgcgtagt tgcggatgta gcggcgggtg cgcgcgatgt
     1141 gccggccggc gcggatgatc cgcggcaggt cctcgcggtc gtcgacccag cgggtgtcgg
     1201 acggcgaccc ggcgtcgcgc agcagcaggg tgccggcctc gcggtcggtc agcgcctgct
     1261 tctccgccgt ctccgcgtcc accgggccgt gcgtgtcgag gccgacgtgg cagtgcgcgt
     1321 cgacgaggcc gggcagcgcc cagccctcga cggtggtcac ctcgagcggt ccggccggcc
     1381 ggtcgtacga gatccggccg tccagcaccc acacctcgtc cctgacgtcc tcggggccga
     1441 ccagcacccg gcccttgacg tgcagcgcac cgcgatcacc catgacactc atgcccagca
     1501 ccctaaaagg tcggaggtac gctcgcgaag gcaccctcgg gcccgacacg gccccgggca
     1561 tgccccagac cttcatccgt acggaagaga cacatcgtga cgcaccccct cctggacctg
     1621 cccccgctga ccgccgcccg tttcgccggg atcgaacggc gtgtcgcggc gctgctgggc
     1681 accgagcagg acgtcgtcat cacccagggc gaggcgctgc tgcccctcga aggctgcatc
     1741 cgcggcgggg cccggcccgg ctccgcggca ctcaatgtgg tgaccggccc ctacgggaag
     1801 acgttcggcg actggctgcg cgacgcgggc gccgaggtca ccgacctggc cgtgccgttc
     1861 cacggggcgg tctccgccga gcaggtggag cgcgctctgg cggagcaccc ggagatcgac
     1921 ttcgtctcgc tggtgcacgc cgaggcggcc accggcaaca cgaacccggt ggcggagatc
     1981 ggcgaggtgg tgcgcgcgca cggcgcgctg ttcatgctgg acgcggtcgc gtcggtggcc
     2041 gcggaaccgc tgctgccgga cgcgtggggc gtggacctgt gcgtcatcgg cgcgcagaag
     2101 gcgatgggcg gccccgccgg cgtgtcggcg gtctcggtca gcgcccgcgc ctgggagcgg
     2161 atggcggcca acccggcggc gccgcgccgc tcgtacctct ccctgctgga ctggaaggag
     2221 cgctggatcg acaccggccg cgccgcactc ccgcacgcgc ccgcgcagtt ggagatgctg
     2281 gcgctggagg cgtgtctgga gcggatcgac gccgacggcc tcgacacggt gatcgcccgg
     2341 caccgggcgg ccgccaccgc gacccgcgag ggcgcgctcg ccctcggcgg cggcctggag
     2401 ccgtatgtgt acgaggcgcg cgacgccgcg ccggtcgcga ccacgctgcg ggtgcccgcg
     2461 gggaccgacg ccaccgcgct ggtggcgcgg gcgctcgccc tggacccctc ggtcccgctc
     2521 gtcgccggcg gtggcgcgct cgccaaggag atggtccgcg tcaaccacta cggccccgac
     2581 gcggatcccg ccgtggtcgc ccggagcctc gcggcgctgg gtacggcgct ttccgaatag
     2641 cgcagcgtca cccattacag cagagcgtga tcacatcaaa ggacaagaaa gcacttccgg
     2701 ggaattcggc ctttctgcgg ccggtccaga ccgaattctc cggaagcatt cttccgcatg
     2761 tctcatgccc agccttccga aggctaaacg caaggattcc gttaaagcat cctggccggc
     2821 tccggcaatg tgacggactc cacaccaaga tcgaatcacc cggtcatatc cggatataac
     2881 cgcaggtctc gccacggttt tggccacccg cacccacccg cgcgataaca cgacgagccg
     2941 gctgcgtaaa gacctccgcc ccatgcaatt ccactttctg ctcggtaggt tcattccgca
     3001 tgaacgccgc tacggaatgg aaggcaccac ttgctcccgc attcctcgac acccccacag
     3061 tcgatgacgg cggagcgatg tggcgcatcg cccgcgattc tcggacgctc gatctcaact
     3121 cctcgtacag ctatctcctg tggtgccgcg acttcgccgg cacctcggtg gtggcccgcg
     3181 acgccgacgg cgccccggtg ggctacgtga ccggatacct ccgccccgag gagccgcgga
     3241 cgctggtcgt ctggcaggtg gccgtcgacg cggcgcagcg cggccgcggc gtggcggcgg
     3301 ccatgctcga ccacctcgcc gcacgcgtcg ccgtcgacga cgtccgctgg atcgagacca
     3361 cgatcacgcc ggacaacgcc gcttcgcagc ggctgttcac ctccttcgcc gagcgccacg
     3421 acgtcccggt cgaacgcgaa gtcctcttcc ccggtgccct gttccccgac gggcacgact
     3481 ccgaggtgct gttccgcatc ggcccgacgg tctgacgcca tcgcgttccg cttcccccca
     3541 cccctgactt cgcaccatcc ccaccgcacc gcacccccac cgcaccgcac ccccaccgca
     3601 ccgcctccac cgcaccgcct cccggaaagc aggcacagtg accatcaccg cacccgacat
     3661 gaccgtcttc gagtccctgg agtcggaggt ccgcagctac tgccgcagct ggccggtcat
     3721 cttcgaccgc gcccagggca gcgtgatgta cgacgaggac ggccacgcgt atctcgactt
     3781 cttctccggc gccggcgcgc tcaactacgg gcacaacaac ccggtgctca aacgcgcgct
     3841 gctggactac ctcgaacgcg acggcgtcac ccacggcctg gatatgagca ccaccgcgaa
     3901 gcgcaccttc ctggagcgct tccagcaggt ggtcctcgag ccgcgcggcc tggactacaa
     3961 ggtgatgttc cccggcccga ccggcaccaa cgccgtcgag gccgcgctca agctggcccg
     4021 taaggtcaag ggccgcgagt cgatcgtctc cttcaccaac gccttccacg ggatgtcgct
     4081 cggctcgctc gccgtcaccg gcaactcgat gaagcgggcc ggcgcgggca tcccgctggt
     4141 gcacggcacc ccgatgccgt tcgacaacta cttcgacggc cgggtgccgg acttcctgtg
     4201 gttcgagcgg ctgctggagg accagggttc cggcctcaac cagccggccg ccgtgatcgt
     4261 cgagacgatc cagggcgagg gcggcatcaa cgtcgcccgt ccggagtggc tgcgggcgct
     4321 ggccgacctg tgcaagcgcc gcgacatgct gctgatcgtc gatgacatcc agatgggctg
     4381 cggccgcacc ggcgcgttct tctccttcga ggaggccggg atcacgccgg acatcgtcac
     4441 gctctccaag tccatcagcg gctacggcat gccgatgtcc ctcgcgctct tcaaaccgga
     4501 gctcgacgtc tgggagccgg gcgagcacaa cggcaccttc cgcggcaaca acccggcgtt
     4561 cgtcaccgcc accgccgcgc tcgacaccta ctgggcggac ggccagctgg agaagcagac
     4621 cgtgatgcgc ggcgagcagg tcgagaccgc gctgcgggag atctgcacgg ctcaggcacg
     4681 cgccggcgcc gagtaccggg gccgcggcct cgcctggggc ctgcagttca tcgacccgtc
     4741 gatcgccggc cgggtctgcg cccgcgcctt cgagctgggg atgctgctgg agacctccgg
     4801 cccgcgcagc gaggtcgtca agctgctgcc ggccctgacc atcacccccg acgagctgga
     4861 cgaggggctg cgcaccctca cgcgggccgt gagcgacgtc gtcggcgaat ccgcctgacc
     4921 caggagccga gtcgagccag gccaggcccg ggtccgcagc cgggcccggc cacgaacgga
     4981 aagaaagcag aggcaaccac gtgatcgtcc gatccttcaa ggacatcgag ggcaccgacc
     5041 gcgatgtgac cgccgagacc ggcacctggc gcagcaagcg catcgtcctc gccgccgagg
     5101 gcgtgggctt ctcgatgcac gagaccacga tgtacgccgg gaccgtgacg tccatgtggt
     5161 acgccaacca catcgaggcg gtgctgtgcg tcgacggtga gggcgagctg accaacgacg
     5221 agaccggcga gacccactgg atctccccgg gcacgctcta cctgctcgac ggccacgagc
     5281 ggcacaccat gcgccccaag accgatctgc gcttcgtctg cgtcttcaac ccgcccgtca
     5341 ccgggcggga ggtgcacgac gagaacgggg tctacccgct gctgaccgag accgggggca
     5401 cgaccgcgac ccgtaccagg acccggaacg ggaccgagac cgagggagcc tgacgccatg
     5461 acgaccacca ccgacaccat caccagccgg tacccgaccc gcggcgcgtc cgaggtcacc
     5521 actccccgcc aggacccggt cgtgtggtcc gagtacggca cgccgggccc gatccagccc
     5581 gccgagctgc gcggctacga ccgggacggc ttcctcaccg tcgaccagct gctcctgccg
     5641 ggcgaagtgg gccgctaccg cgatgagttg gaccggctct gcgccgatcc ggcgatccgg
     5701 gccgaccccc gcgcgatcgt cgagccgaag tccgggtcga tccgctcggt cttcgaggtg
     5761 caccggatca gcgaggtctt cgccgagctg gtccgcgacc cccggctcgc cgaccgcgcg
     5821 cgccagatcc tcggctccga cgtctacgtc caccagagcc ggatcaacgt caagccgggc
     5881 ttcggggcgt cggggttcta ctggcactcg gacttcgaga cctggcacgc cgaggacggt
     5941 ctgcccaaca tgcggacggt ctccgtctcg gtggcgctga ccgagaacca cgacaccaac
     6001 ggcgggctga tgatcatgcc cggctcgcac cgtacgttcc tcggctgctc gggcgagacg
     6061 ccgaaggaca actacaagcg gtcgctgcag atgcaggacg ccggcacccc gtccgacgag
     6121 gcgctcaccg aactcgccgg ggagcacggc atcaagctct tcacgggtgc ggcgggttcc
     6181 gcgacctggt tcgactgcaa ctgcatgcac ggttcgggcg acaacatcac gccgttcccg
     6241 cgcagcaacg tcttcatcgt cttcaacagc gtggagaacg cggcggtcga gccgttcgcg
     6301 gcgccggtac ggcggccgga gttcatcggc gcacgggact tcaccccggt gcggtagcgg
     6361 gcaagccctg cgggggccgg actgctgggg gcagcccggc ccccgcaggg cgtttcagcc
     6421 gccgtcgcgc aggtcggcgg gcgcgccgga ccggaaggtg atccggtcga agaccgccgt
     6481 gcacccgtcg gcgtccggcg acgggcactg cgtcatgaaa ccgacgctcg cggccgccgc
     6541 ctcccgctcc gtgccgaggg cgaagagccg gacgaacgtc caccgctcac cgtcggccga
     6601 ggcgtggaag gcgaaggccc ggccggtgcg ggtgatccgc agccagacgt ggtccccgtc
     6661 cacctcccag ctgttggcgt cgtcggagct gccctgggtg acgaccgaga cgacggtgta
     6721 ccgggcgggc gagagctcca ggcagagctt ggcccactgc tcgtcggcca cctgcaggta
     6781 gagcgctccg gcgtcgccag cggcccgcag ccgggccgtc acccgggcga tcagctggaa
     6841 gtcgccctcg ggcggggtgc cgagcagccg gggcgcgtcg gccgccggta ccttcggcgc
     6901 ccggccgggg tgcacgaagc ggtcctggcc gggacccgcg gtgaggacga gcgcgccgtc
     6961 ctggtagctc ggatcggcgg ccggaccgta cgcccgcagc ccgaagggca gctccttcag
     7021 ctccactacg cgttcagctc cgctcagatc tccagccggc cgtcgaagcg gcgcggcagg
     7081 tggagcgggt tctcgtcgcg cagctccgcg gggagcaggg cgaccggggt gtcctggtac
     7141 gcgaccggcc gcagccagcg ctcgatggcg gtcccgccga cggaggtcga ggtcgacgtg
     7201 gtcgccgggt acgggccgcc gtggtgctgg gcgggggcga cggccacgcc ggtcggccag
     7261 ccgttgacga gcagccggcc cgccagcggg gtgagttcgg cgagcagcgc ggtggccggg
     7321 ccgtcctcct gtcccgcctc ctcctcaccg atgtggacgg tggcggtgag gttgccgggc
     7381 agccgctcca gcacggcggt gatctgggcg cggtcctcgt agcgcgccac gaccgtcacc
     7441 gggccgaagc actcctcgag cagcagatcg tgcggtcctt cggcggccag ccggtcggcg
     7501 ggaacggtga ggtatccggc gctgacggtg tgctcgccgc cggagcccgg ggtcaccggg
     7561 gcctcgacgc cgggcagcgc ggccctggcc tcgacaccgg cgacgaaggc gtcccgcatc
     7621 cggacgtcga gcaggacgcc cgcgtccgag tcgctgacgg cggcggtgac ggccttgagc
     7681 agccggtcgc ccgccgcgcc ggccggggcc agcaccagtc ctggcttgac gcagaactgg
     7741 ccgacgccca gcgtcatgga cccggcgagt cccgcgccga tctgctcggc ccgctcgtcc
     7801 gccgcggcct cggtgatcac gaccgggttg aggctgccca gttcgccatg gaaggggatc
     7861 ggccggggac gggccgcggc cgcgtcgaag agcgcccgcc cgccgcgtac cgagccggtg
     7921 aatccggcgg cgctgaccag cgggtgcttg atgaggtcga taccggcctg gaagccgtgc
     7981 acgacggcga cgacgtcggc gggcagcccg acccggacgg cggcgcggcg cagcacgctc
     8041 gcgcacagct ccgaggtggc ggggtggtcc gggtgcgcct tgacgacgac cgggcagccg
     8101 gcggccagcg cgctcgcggt gtcgccgccg gggacggaga acgccagcgg gaagttgctg
     8161 gccgagtaga ccgcgaccac gccgagcggg atcttgtagc gccgcagttc ggggcgggga
     8221 gcgggcttcg cggccgggtc cgcgtggtcg atgacgacgt ccaggaaggc gccctcgtcg
     8281 acgatgtcgg cgaacgcgcg cagctggaac gtggtgcggg cgagctcgcc ggtcagccgg
     8341 ccggggccga gcgccgtctc ggcgtcggcc gcctccagca cctgcgggcc ggcctcctcc
     8401 agaaggtccg cggcggtccg cagcaggagg gcgcgtacgc tgcggtcggc gagcgcgggt
     8461 gccgcggcgg ccgcggcgcg gacggcggcg tcgatctccg ccgtggtggc ctcgtccgcc
     8521 acctgctccc gctgcttccc ggtacggggg tcgacggacc agactggaac agccaccggg
     8581 ggaacctcca gatttgcctc atgcggatca gccactgttc gatatgctga acagcgtctt
     8641 cacttatgaa tctttgaacc attccggagc ctacttgcag gatccggggc cgaacaagag
     8701 gggtcaggac gatgtctgct ggcgacggtg gcggttctca ggtcaagtcc gcggtccgca
     8761 cggtcgaact gctggaattc ttcgcgggac gacccggaat gcacagcctc gcggccgtac
     8821 aggaatccgt cggctacccc aagtccagcc tctacatgct gctccgcacc ctagtcgagc
     8881 tcggctggat cgaaaccgat gccaccggaa cgcgttacgg gatcggtgtc cgggcgctgc
     8941 tcgtcggcac ctcgtacatc gacggtgacg aggtcgtcgc cgcggcccgc cccaccctcg
     9001 accggctctc cgacgacacc agcgaaacca tccacctcgc ccggctggac ggcaccagcg
     9061 tcgtctatct cgcgacccgc cagtcccagc actacctgcg gcccttcacc cgggtcggcc
     9121 gccgcctccc ggcgcactcc acctcgctcg gcaaggcgct gctcgccacg tacaccgacg
     9181 agcaggtccg caaactgctc cccgagaccc tcgaacccct caccgagcac acccacaccg
     9241 accgcgagaa gctcatcgag gaactcgcgg tcatccggga gcagggcttc gccgtcgacc
     9301 gcgaggagaa caccctcggc ctgcgctgct tcggcatcgc catcccgtac cgcaccccgg
     9361 cccgcgacgc ggtcagctgc tccgtccccg tggcccggct cacccccgcc cacgaacaga
     9421 tgatcaagga caccctcttc gacgcccggg accgcctgac cctggccacc cgccggctgt
     9481 gacctttcgc atcgtcacag aacgtcgacg acctgcccct cacctcgttg cccccgctcc
     9541 cggaatgcaa gaatcgacgc ggagttgatc acacacgggg gaacaacgtg gcgaatcgtc
     9601 cgacggattg gcatgtgctg gacctggacc gggatccggt ccccggggac ccgtacgagg
     9661 tgaaggaact cgcccgcaaa ctgggcgact tcgccgacga cgtgtcctcg gcgctgcgct
     9721 cggtgcgcgg gctgagcggc aacaacgccg tgcaggactg ggccgggctg gcggcggacg
     9781 cgtaccggga gcagttcggg gatctgccgg gcgagctggg caagttggag aagtcctacc
     9841 ggctggcgtc gggggcgctg gagacgtact ggccgaagct ggagacggcg caggccgacg
     9901 ccgaccgggc gctggcgcag ggtcgtacgg cccgccagga cctggacgcg gccaagacca
     9961 cccagacgaa cgccaacgac tgggtgaagc gggcgggcga caagtccaag gagtaccagg
    10021 ccgatccgaa gccgggcgtg gaaccgccgt ccgcggacga ggtccgtacc gccacccgca
    10081 acgcgctgga cgcctcgaac gcgcagaagt ccgcgaacac cgccgtccac gacgcgcagc
    10141 agcgcctcga cgcggcaaag gagttggccg cgcaggccgc ccacttgcgg gacaccgccg
    10201 cctcgaccgc cgaacacgcc ctgcatgagg cgtcggacgc ggggatcaag aacaagcact
    10261 ggtgggaaaa ggcggtcgac tgggtcgccg accactggga cgacatcgtc gccgtctgca
    10321 aggtcatcgt cgcggtcctc ggcatcatcg tgatgatcat cggcggaccg ctggcctgga
    10381 tcgtgctcgc cgcggcgctg gtcgtccttg ccgacacgct catcaaatac gcgcaaggca
    10441 aagcatcgct gtg
//
