LOCUS       NZ_CP048835            21044 bp    DNA     linear   CON 17-MAY-2024
DEFINITION  Streptomyces sp. So13.3 chromosome, complete genome.
ACCESSION   NZ_CP048835
VERSION     NZ_CP048835.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces sp. So13.3
  ORGANISM  Streptomyces sp. So13.3
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Quezada-Solis,D., Khalil,Z.G., Capon,R.J.,
            Andreote,F.D. and Barrientos,L.
  TITLE     Genomic and Metabolomic Analysis of Antarctic Bacteria Revealed
            Culture and Elicitation Conditions for the Production of
            Antimicrobial Compounds
  JOURNAL   Biomolecules 10 (5), 673 (2020)
   PUBMED   32349314
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K., Lamilla,C., Abanto,M., Maruyama,F., Jorquera,M.A.,
            Santos,A., Martinez-Urtaza,J. and Barrientos,L.
  TITLE     Antarctic Streptomyces fildesensis So13.3 strain as a promising
            source for antimicrobials discovery
  JOURNAL   Sci Rep 9 (1), 7488 (2019)
   PUBMED   31097761
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9564008)
  AUTHORS   Nunez-Montero,K. and Barrientos,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2020) Centro de Excelencia en Investigacion
            Biotecnologica Aplicada al Medio Ambiente (CIBAMA), Universidad de
            La Frontera, Av. Francisco Salazar 01145, Temuco, La Araucania
            4811230, Chile
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JAN-2020
            Assembly Method                   :: Unicycler v. OCT-2019
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140.0x
            Sequencing Technology :: Illumina MiSeq; Illumina HiSeq; Oxford
            Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005860785.2-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 04:03:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,707
            CDSs (total)                      :: 8,616
            Genes (coding)                    :: 8,274
            CDSs (with protein)               :: 8,274
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 342
            CDSs (without protein)            :: 342
            Pseudo Genes (ambiguous residues) :: 0 of 342
            Pseudo Genes (frameshifted)       :: 109 of 342
            Pseudo Genes (incomplete)         :: 268 of 342
            Pseudo Genes (internal stop)      :: 24 of 342
            Pseudo Genes (multiple problems)  :: 52 of 342
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:45:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1516537
            Orig. end                         :: >1537581
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria and source DNA available from Laboratorio de Biologia
            Molecular Aplicada, Universidad de La Frontera, Avenida Alemania
            0458, Temuco, Chile.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..>21044
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="7"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..>21044
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..>21044
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:>11044](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..>11044)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(1352..2419)
                     /locus_tag="C8250_RS07095"
                     /old_locus_tag="C8250_007115"
     CDS             complement(1352..2419)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018349.4"
                     /locus_tag="C8250_RS07095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007115"
                     /product="DUF1152 domain-containing protein"
                     /protein_id="WP_138355507.1"
                     /transl_table=11
                     /translation="MSDLLIAAGGGGDPVGTAIVHAATSRDPHAVIATYAWERLEVDPT
                     PGPLGPAAFTGWGESAGGHRFTGRTTAVPPAGSTLPRLSESLGPSLLLLDPYQGVPGLA
                     RQIRRAADASGCGQVRIVDIGGDILTHGDEPTLQSPLPDALTLAACATAGVTASVHVAG
                     PGLDGEIPEGVLLPRLTGGHLTPTDQDVRAAAAALAWHPSEASALFAAAATGRRGTVRT
                     VGAEVRLTPASGQVHHLTLDQALAHNPLARALYQEQPGSLEDAERLALRTCGFSELARE
                     REAAERLSATAPASRAVPVPTSRDQVLYMLWQSAPGAGCVTFRYAARVLGLSWRDIPRL
                     RELTKADGPLLPLVM"
     gene            complement(2416..3885)
                     /locus_tag="C8250_RS07100"
                     /old_locus_tag="C8250_007120"
     CDS             complement(2416..3885)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127145.1"
                     /locus_tag="C8250_RS07100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007120"
                     /product="radical SAM protein"
                     /protein_id="WP_138355508.1"
                     /sec_met_domain="PF04055 (E-value: 1.6e-16, bitscore: 60.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNPTDQLADCIAQGVIPPYNYSYPPRSTYRPLAPGQWDLQELWA
                     QDLTKYQVPELNLYLHVPFCRYKCGFCNLYTVISTDQDLYDAYTDALCTQIRDHAEIIQ
                     ARRLRTVYIGGGTPALLSLRHFEQIFATIAEVYPNWRSTVEEVAVEATPDSIVADPDGF
                     EGLIRLGLTRANIGIQSLVPQEIREAGRGQAGQDVIREAIRIVQDKGLPDLSTDLIMGF
                     AGQTDESWRHSVDELAALAPTTISTYFLTVRPDAWFSRTGAYQYMWKPELYDRYDYARE
                     VFTEHGYVQEGSVRYKTPGRGGYVQKVLTFHGVPLLGLGVGARSYTGVLDYMTGSVKPS
                     MSEVADYITQARNHSLVPTTGFALTPEEIVRKRLVLDSFDLDLGELDAIGLAGLRDRID
                     PVLEAAEANGLIHRVGPRRIQLTPKGFKYRDILSWSFFSDRVIELDREHFENLHDRNSR
                     AKKNMGTPVRITGVTTQENLQEKNEQREKSA"
     gene            4104..5357
                     /locus_tag="C8250_RS07105"
                     /old_locus_tag="C8250_007125"
     CDS             4104..5357
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /locus_tag="C8250_RS07105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007125"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_138355509.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 5e-08, bitscore:
                     32.1, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 5.5e-09,
                     bitscore: 34.8, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHRTWGNATAKIFVVEPLGSSGRFLTAAARDLGAVVHAGTHKDIY
                     DSYPAGLRAELAGVCRTDLADPDRALDEMEAYCRAEGVDAVAACWELLTPLATRLAHRL
                     GLPGNDPDRAAASRNKIAMAAALEHAGVSVPRTVVAGTGDEALDLIGAADLAFPLVIKP
                     AEQGGSWGVSVVGGPDEVRAAVDAAQQYTVAAPHGLSLDPRVIVQEYVDGPEYSADTVV
                     QDGRPFPLPVVRKHTTVDAYRIEIGHTCPAPLPHAAAHEIQAVAARAALAVGIRNGVAH
                     TELKVPPGSGRPVVIETGARLPGGNICEVVEHATGISEARAYLRVVLGQAPETTALRDA
                     ACAIRFLVPPRAGILRTLRTADLPGVYSEFTVRPGDPLRAPVDNSCRIGHVIATAPNSA
                     QAELLADRAISQTTIEVA"
     gene            5361..6617
                     /locus_tag="C8250_RS07110"
                     /old_locus_tag="C8250_007130"
     CDS             5361..6617
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /locus_tag="C8250_RS07110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007130"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="WP_138355510.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 5.2e-09, bitscore:
                     35.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHKIAFLRSVDIQRADPYIQQAVRELTADGIESRLFYTHGTCGPA
                     DFPAGGEHLDPAVTPLELADAVSAWGADTAVSVSLPCENALRDATAKAFLDARGIPTVM
                     TPLAATWLLCDKWETKQALIAAGLDVARGIRADSDLTAGRGLPVPAYRQSLLVAAGEIG
                     YPLLSKPLWDSTSMGIRFLPDRAALDAYLADPPPVSAVLEECLTGELCSVDIVGAPGNY
                     SVLPLCWTGQAGGGAAFTFADLRWCGPRPQADADFAPVAERLVGLCESLGVYGSVNVDM
                     IHVNGRYVVLEINPRIGGATTLSCAASGTNTFATLADMARGRYPDAARPDTRRKRWAIE
                     FLADRPVGQAADELRDRLGLVTAHELVIDGTSHGDIVTFTCEEGDEQRTVKELDTINSH
                     SGILAPVMMGKVRALLCGL"
     gene            6614..7888
                     /locus_tag="C8250_RS07115"
                     /old_locus_tag="C8250_007135"
     CDS             6614..7888
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="C8250_RS07115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007135"
                     /product="MFS transporter"
                     /protein_id="WP_138355511.1"
                     /transl_table=11
                     /translation="MTGATGRRAGRGRAARIAGVAGLPELRGRGRFVVAHVIDSIGNGL
                     LLPLGLLFFITVRHIPAATAGAAASTGQLAALPLTALAGRIMDRAGPKYLAVASNLVCA
                     AGFGGFLVAERPWQIAAAYFTVQAGINGYYTSQRSLTLLVAAGDEPRTWFAFTSSLRNT
                     GLAAGAAIAAAALALWGTGALTALVVADAASYCLAATLFATLNVASGRPNAPPGRTTWS
                     IDRRYLALVLCNLPYVFAQAILSVLLALYATSALRLSAWSASVLLLVNTVAVALTATAV
                     TAHLAPRAARHAVSVAYAVLALSMAAFAAPALPGLRPLAWPALAVGIVLFTVGEILCSP
                     AMAELSTTLGPAAARGSHQALFQMSWSVGGAAAPVLFTVLLHRSSLLPWAFEAILCVLA
                     ALAVLPLVPSGSAFPSSRPRRKRTR"
     gene            7885..8898
                     /locus_tag="C8250_RS07120"
                     /old_locus_tag="C8250_007140"
     CDS             7885..8898
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127151.1"
                     /locus_tag="C8250_RS07120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007140"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_138355512.1"
                     /transl_table=11
                     /translation="MNLESVPTLAAVPAAEWDHLARDTSFYLSHRWLAGEETDPSATCT
                     YLLVRDPHGRLTAAAPLYLVHDEPNTNYRPEHHLDVGQDEAPRVIAGARRGYHNAPLLA
                     PGLDDRQRAGCLTMLRDAAREHAARQSTWHWWPYLTDRAATELGDAYGTAPRRIEDDAT
                     ISLPGTGFDDYLAALPAKRRSGIRGERRAFDARALTVRTEPLANCVDDAGRLLAILQCR
                     HGHVTDTAAMTGVMRRQAQAMGEDAQVTAAYDGATMVAFSLVYHFGNTAWLRAVGHDPQ
                     HTGDAFEYFNLAYYLPIEDAYRHGCTHLHAGMKSLAAKRARGAHTSPLWALADPPR"
     gene            complement(8992..9858)
                     /locus_tag="C8250_RS07125"
                     /old_locus_tag="C8250_007145"
     CDS             complement(8992..9858)
                     /EC_number="2.1.1.255"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0042214 - terpene metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433228.1"
                     /locus_tag="C8250_RS07125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007145"
                     /product="geranyl diphosphate 2-C-methyltransferase"
                     /protein_id="WP_138355513.1"
                     /transl_table=11
                     /translation="MTIDHITAPAPLLNTSPYQRSVAAYWNAEKNPVNLRLGDVDGIYH
                     HHYGIGDVDWSVLDASEDMRENLVTKELHRLECNQTEFLLDQFGAIGPEDRLLDAGSGR
                     GGGSIVANQRFGCSVDGVSISQSQVDFANDQAVKRGVDDKVRFHLKNMLSTGFDSGTFR
                     GIWNNESTMYVDLAQLFAEHSRLLARGGRYVTITGCYNDTYGLPSRAVSQINAHYICDI
                     HPRSTYFKEMAANRLVPISVVDLTSATIPYWELRAKSSLVTGIEDPFLTAYKDGSFQYL
                     LIAADRV"
     gene            complement(10001..>11044)
                     /locus_tag="C8250_RS07130"
                     /old_locus_tag="C8250_007150"
                     /pseudo=""
     CDS             complement(10001..>11044)
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041167.1"
                     /locus_tag="C8250_RS07130"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007150"
                     /product="family 2 encapsulin nanocompartment cargo protein
                     terpene cyclase"
                     /pseudo=""
                     /sec_met_domain="T1TS (E-value: 2.2e-56, bitscore: 191.1,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="QPHPAPATAQPPALAPAPTPTSAPAPARIEGLHCPPAVRDDRTLA
                     DLINEQLLQWADDVDLFRSPDLRAGLLKFDPGRSVVLCHPDAPTTGHLLTAAKLLAGEN
                     AVDDYFCEPELGGTPDGLGARLAVAQSAIDPSHMPAPYDADWRAALAAHPALRAMHSAM
                     ESFTQLATPAQSHRYRHEIASLYLGYEAEAACVQEERTPRVWEYLVQRQLNSFRPCLAI
                     TDAIGGYELPAELFALPSVQRATALASTASTVLNDLYSLRKEQASGRFHYSLPTVIAEE
                     EDCAIEDAFTKAIEVHNELVRAFETEAAALTAHPVAARYVAGLSNWMGGNHEWHAGHVG
                     GRYATSP"
     misc_feature    complement(11370..11385)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.61"
                     /tool="antismash"
     misc_feature    complement(11522..11536)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.39"
                     /tool="antismash"
     misc_feature    11542..11553
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     misc_feature    complement(11543..11552)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.55"
                     /tool="antismash"
     gene            11574..12992
                     /locus_tag="C8250_RS07135"
                     /old_locus_tag="C8250_007155"
     CDS             11574..12992
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174667.1"
                     /locus_tag="C8250_RS07135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007155"
                     /product="family 2B encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_187144764.1"
                     /transl_table=11
                     /translation="MSIEARPESAPAQGNSPSHTSLDTAAARNLATTTKSAPQMAGISS
                     RWLLRMLPWVEVTAGTYRVNRRLTYSVGDGKVTFVKTGSQVRVVPAELGELALLRSFED
                     TAVLTALADRFTQREFAPGELLAEAGQPVDTLFLLAHGKVEKLSEGEFGEQNRLGVLAD
                     GAHFGEQGLLDPETPWDFTARAATTTTVLALSRQDFQTLADQSPALRAHIEAYRAIPRQ
                     RVNKRGEAAIELASGHKGEADLPSTFVDYEVAPREYELSVAQTVLRVHSRVADLYNEPM
                     NQIEQQLKLTIEALRERQEHELINNREFGLLHAADYDQRIQPHAGPPLPDDLDELISRR
                     RGTRFLLAHPRTIAAIGREWTRKGLYPGNAEVDGSSVLAWRGVPILSCGKIPISEARTS
                     SIIAVRTGEDNQGVIGLRQTGIPDEYEPGLNVRFMGLSEKAIISYLVSTYYSAAVLVPD
                     ALGILENVNIANWR"
     gene            complement(13035..15179)
                     /locus_tag="C8250_RS07140"
                     /old_locus_tag="C8250_007160"
     CDS             complement(13035..15179)
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843847.1"
                     /locus_tag="C8250_RS07140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007160"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_138359445.1"
                     /sec_met_domain="Pkinase (E-value: 8.8e-39, bitscore:
                     132.4, seeds: 54, tool: rule-based-clusters)"
                     /sec_met_domain="APH (E-value: 5e-07, bitscore: 28.9,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSPMATPATPAPGSAPHIGPYRVIRELGAGGMGRVYLAASRSGR
                     AVAVKVVRPELTSDPGFRRRFTAEVDAARAVGGAFTAPVVDADPEGPQPWLATEYIPGP
                     SLSEAIQAHGPMPEATLRVLGAGLAEALAAIHRVGLIHRDLKPSNILLARDGPRVIDFG
                     ISRAVDGTVLTADGQIIGSPGYMSPEQSTGAELTPASDVFSLGSVLAFAGTGAPPFGTG
                     AAHALLYRTSHEEPRLEGVPYALLGILTACLDKDPARRPPVGQLQVWLRTVTTAGWLGA
                     VELQVNASERYLHEAVRAPLFSRRRLLAGGGVLAVAAAAGGTALLLRSGGRGATATPEL
                     SWSADLPQPDMALRAVTPSALLCAGKTSGACYDRSTGNALWKDLSGQNTAAIADDRHVY
                     TVRTDGKIHALDVRSGKQLWERPATATGAPHLEFANGSLLVAMVDSGHFQGLDASTGAE
                     RWKSPEGDGIAVTGTTRAGGLVLWGSTSQTSTTASLRGYTTVAPDTGARLWAKDLVALY
                     PPSEGDVLYALDGAMNLLALKPADGATLWSRATSLPPTNSQILAYDGSLELHQGTLYCY
                     PGTDASGAVSGLLAAFDPSTGKTLWSVQTAARGNRGYDRSGATVCYLDSTLHAVDALTG
                     APRWTAGSGLGTLQFLGSARGLFLAAAPGGLYGFHAGTGKQVWHYPVSGGSGYWAAFPV
                     DDHLYVSSSGKLLCFSVPEA"
     gene            complement(15176..17407)
                     /locus_tag="C8250_RS07145"
                     /old_locus_tag="C8250_007165"
     CDS             complement(15176..17407)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182058.1"
                     /locus_tag="C8250_RS07145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007165"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_138359447.1"
                     /sec_met_domain="Pkinase (E-value: 5.4e-40, bitscore:
                     136.4, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTPAYGANDPTAPRQRTTIQEQGQEQRQPQSQSPGRIGPYEVLQ
                     LLGEGGMGRVFLARSPGSRLVALKVIRPEYTEAPDFRGRFRREADAARKVSGFFTPPVL
                     DADAGAEQPWLATAYIPAPSLHDAVARFGPLPEPALRTLGAGLAEALLTIHAAGIVHRD
                     LKPGNVLLAHDGPRVIDFGISRVAEATQVTRTNAVMGTPGFMAPEQIASSRETGPAADV
                     FSLGCVLAFAATGGGPFGTGSTPGILYRAVHAPARLDGVPDALRPLVAACLDKEPARRP
                     SVTALLTALGPVEPSSLLTPGLLADIAAREASAAALLAVPPIPVGPPDTAAPGGPSRRR
                     FLWIAAGGTAVAAASATALIDRRSKPGRSPGGAPGSAGTTIRPGAKVAAGPKPQWSMPL
                     QQLQAGAHLELLGGTLVHWDKTTATGYDSATGQKRWTADPRVPSDVSGKLEWLGVRGST
                     LFASAWGGERGHLLGVDGGGTLRFTHAVTGPEDGDSYADSIHDVFFVARSVALIGTSGD
                     RAYQVRAVDLVTGKLLWSHKVKGSDFHAYSDGVGCFLHDGSDVHGVDLRSGAVRWTVRD
                     VVRPGDYPGLASDGKALLLTSVKVQAFRASDGHRLWTAVNSTTTLNPTTVLGQRAYVVA
                     EGQGTVFALDTGSGDQVWHTASPLTLDPQNSLNPGPAVSTSLVAVPLFAAGDTPGFLVL
                     RASDGKLLWAHQPSGAESASKNAWLLQAVGSTVYAASDTTLYAFRSDTS"
     gene            complement(17425..18240)
                     /locus_tag="C8250_RS07150"
                     /old_locus_tag="C8250_007170"
     CDS             complement(17425..18240)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018538123.1"
                     /locus_tag="C8250_RS07150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="C8250_007170"
                     /product="hypothetical protein"
                     /protein_id="WP_244809004.1"
                     /transl_table=11
                     /translation="MPGGAGLGTGGAAPGQLPPPVPAQPPGAPSAWSQHLPPTPPQYGP
                     PNGGRPGGSGKPWLIGGAVLVVAAIVGVIIATSGGDDKNAASGSPSGAPGGGKLPSQAP
                     AGRTFTHVPDGCRLIKEATIASIAPGTECKPSQFDNTTMAAMITKMPSWDTPSGSGGRL
                     LHLRVHLTVSPGAKGMYDMDKSTALNALKDMRTTTDSRSLTGLGEEAYVVHAVDKDMGG
                     LAEATVIVHEGNAAFSVSFGYDTSDSGPRQQQSEDAAVAAARDVLGSLS"
     misc_feature    complement(18804..18817)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.86"
                     /tool="antismash"
     misc_feature    20245..20260
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.73"
                     /tool="antismash"
     misc_feature    20593..20608
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.39"
                     /tool="antismash"
     misc_feature    complement(20626..20640)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 26.66"
                     /tool="antismash"
ORIGIN
        1 ttcctgacgg tgcatctgat aggggctgaa gatcaccgtg gtacccctgg gaaggaccgt
       61 gtcgccgagt cggctgtcct gagtcacatt gcgggtgaag agccagcctg gcgggtacag
      121 acgcagcgcc tcggagatga cgcggccggt cagctccagc cgcggcaggt cgtgccactc
      181 ggccgctctg ccgcgcagga cggtgtggac ctcggcgcgt acgcgctcct ggatcccggg
      241 gttgccggcc aggaggtggc aggcccaggc catggtcgat ccggtggtgt cgatgcccgc
      301 catgaggaag gacatgacct ggtcatggat ctcggacagg gccaggccgt ccgtgccgtc
      361 ctcgtccgag ccatcccggt ccccgtccct gtcgtcgtcc ctggcctcca acagcaggga
      421 cagcatgtcc tcgtggcgct ggccgcgtcg gcggtagtcg ctgatgatcc cgtcgatcgt
      481 cgcgttcagt gtggcctggg cccgcgcgca gcgccggctc cacggggcca gctgcctgcc
      541 gagcggcgcg aacatgttcc agaacccggc tttgacgatg atgtcgatgc accgcaccat
      601 ctccgcgatc gccgcttcgt ccggcccggc ggcgaacagc gtcgcggtgg ccacccgcgc
      661 cgagttcgcg tgcatgcacg agaaggcgtc gatggtctgc ccgtccgtcc atgagcgggt
      721 catggtgtcg atgtgcccgg tcatcgtcgc ggcgtaggcc ggcaggcgat cgcgccggaa
      781 ggcgggctgc agcaggcgtc gctggcgccg gtgagcctgg tggggacagg tcaccaggcc
      841 ctctcccaga gtgcgcttgc tctgctcgta gatcggtccg gccttgtcga aggtccggtc
      901 gtccatcagc acccggtgca ccaggtccgg atggcacacg accaggccgt gccacggacc
      961 gatgccgatg cggaccaggt ccgcgtgggc ggacagcgag gtcaggaacg ccagcggatc
     1021 cttcatgatg cgcggcgcgt ggcccaggag ccaccacctg ccgggcgccg tgcccgtcgt
     1081 gtaggccttc gcctcctggt gcccgccgtg tgccgcgccg gtcacgcgag cacgcggcgg
     1141 gccggagccg gcgacattgc cgtccgcggt gccgcgtgct cggcactgcg ggtccggggg
     1201 gcggtgcggt ggtgctggga catacggttc ctcgcggcgg gagccttcgg accgactgcg
     1261 gatcggtcgg acgaggtgag aactctccaa ggaaaccgaa gttacgtcgc cgggccgcag
     1321 agcactcgaa ggaggaggaa cccctgacgg ttcacatcac gaggggcagc aacggtccgt
     1381 cggccttagt gagttcgcgc agccgtggga tgtcccgcca ggagaggccg agaacgcgcg
     1441 ccgcgtaccg gaaggtgacg cagccggctc ccggtgcgga ctgccagagc atgtacagga
     1501 cttggtccct gctggtgggt acggggaccg ctcgcgaggc gggcgcggtg gccgaaaggc
     1561 gttcggcagc ctcgcgttcg cgggccagtt ccgagaagcc gcacgtccgc agagcgaggc
     1621 gttccgcgtc ctccaggctt cctggctgtt cctggtacag ggcccgggcc agcgggttgt
     1681 gggcgagcgc ctggtccagg gtgaggtggt ggacctgccc gctcgccggg gtgagccgga
     1741 cctcggcgcc caccgtccgt accgtgccgc gccggccggt ggccgccgcg gcgaacaggg
     1801 cggaggcttc cgagggatgc caggcgagtg ccgctgccgc ggcccggacg tcctggtccg
     1861 tcggcgtcag gtgtccgccg gtgagccgag ggaggagaac cccttccggg atctcaccgt
     1921 ccaggccggg acccgcgaca tggacgctcg ccgtgacccc ggcggtggcg caggcggcca
     1981 gcgtcagtgc gtcgggaagc gggctctgca gagtgggttc gtcgccgtgg gtgaggatgt
     2041 cgcctccgat gtccacgatc ctgacctgcc cgcagccgga ggcgtcggcc gcacggcgga
     2101 tctgccgggc gagtcccggc accccctggt acggatcgag caggaggagc gacggaccga
     2161 ggctctccga caggcgcggc agggtggagc cggccggtgg aacggcggtc gtccgcccgg
     2221 tgaaccggtg tccgccggcg ctctctcccc acccggtgaa ggcggccggc cccagcgggc
     2281 cgggcgtggg gtcgacctcg agccgttccc atgcgtaggt cgcgatgacc gcgtgcgggt
     2341 cgcgggaggt ggcggcgtgc acgatggcgg tgccgacggg atctccgccg cctcccgcgg
     2401 cgatcagcag gtcgctcaag cggacttctc ccgctgctcg ttcttctcct gcaagttctc
     2461 ctgcgtggtg acgccggtga tccgcacggg agtgcccatg ttcttcttcg cgcggctgtt
     2521 cctgtcgtgg aggttctcga agtgctcgcg gtcgagttcg atgacccggt cggagaagaa
     2581 cgaccacgac aggatgtcgc ggtacttgaa ccccttgggc gtgagctgga tcctgcgcgg
     2641 tccgacacgg tggatcagcc cgttggcctc cgccgcctcc aggacggggt cgatgcggtc
     2701 gcgcagcccg gcgagcccga tggcgtccaa ctcgcccaga tccaggtcga agctgtccag
     2761 gaccagccgc ttgcggacga tctcctccgg ggtcagcgcg aacccggtgg tcggcaccag
     2821 ggagtggttc ctggcctgcg tgatgtagtc ggcgacctca ctcatcgacg gcttgaccga
     2881 accggtcatg tagtcgagca cgccggtgta ggagcgggct cccaccccga gcccgagcag
     2941 cggcaccccg tggaacgtca gcaccttctg cacatagccg ccccggcccg gtgtcttgta
     3001 ccggacggag ccttcctgca catagccgtg ctcggtgaag acctcgcggg cgtagtcgta
     3061 gcggtcgtac agttccggct tccacatgta ttggtaggcg ccggtcctcg agaaccaggc
     3121 gtcgggccgc accgtgagga agtaggtgct gatcgtggtg ggcgccagcg ccgcgagctc
     3181 gtcgaccgag tgccgccagc tctcgtcggt ctgtccggcg aaccccatga tgaggtccgt
     3241 ggacaggtcc ggcagcccct tgtcctggac gatccggatc gcctcgcgga tcacgtcctg
     3301 cccggcctgg ccgcgcccgg cctcgcggat ctcctggggc accagcgact ggatgccgat
     3361 gttcgcccgg gtcagcccga ggcgtatcag cccctcgaag ccgtccgggt cggcgacgat
     3421 cgagtccggc gtggcctcga cggcgacctc ttcgacggtg ctgcgccagt tggggtagac
     3481 ctcggcgatc gtggcgaata tctgctcgaa gtgccgcagg gacagcagcg ctggggtgcc
     3541 gccgccgatg tagacggtcc gcaggcggcg cgcctggatg atctcggcgt ggtcacggat
     3601 ctgggtgcac agcgcgtcgg tataggcgtc gtagaggtcc tgatcggtgg agataacggt
     3661 gtagagattg cagaagccgc atttgtagcg gcagaagggc acgtgcaggt acagattgag
     3721 ctcgggtacc tgatatttcg tcagatcctg tgcccacagt tcctgtagat cccactggcc
     3781 gggagccagc gggcgatagg tggaccgcgg cggataggag tagttgtagg gcggaatcac
     3841 accttgggct atgcagtcgg cgagctggtc ggtcggattg ctcacggatt ccctctcctg
     3901 ggcgtgaggt catcgaagtt gaccttgtgc gctccactcc acctcaccgg cgaccggttc
     3961 ctccataggc ggcatggccc aacttggcct gccctactgg cgagttgttc ataggtatct
     4021 ccaacaaccc cggtcggacg agcacgatct ttcggacgac gtcccgatgg accgccggac
     4081 cgcatcccgg ttttagtggt ccaatgcacc gcacctgggg gaacgccaca gcaaagatct
     4141 tcgtcgtcga gccgctcggc agttccggcc gctttctcac cgcggccgcc cgggacctcg
     4201 gcgcggtcgt ccacgcgggt acgcacaagg acatctacga cagctacccg gccggtctgc
     4261 gcgcggagct cgcgggtgtc tgccgcaccg atctcgccga ccccgaccgc gccctggacg
     4321 agatggaggc ctactgccgg gccgagggcg tcgacgcggt ggccgcctgc tgggagttgc
     4381 tcactccgct cgccacccgg ctcgcgcacc gcctcggcct ccccggcaac gaccccgacc
     4441 gggccgcggc atcccggaac aagatcgcca tggccgccgc gctggaacac gccggcgtct
     4501 ccgttccccg caccgtcgtc gccgggaccg gcgacgaggc actcgacctc atcggcgccg
     4561 cggatctcgc cttccctctg gtgatcaaac cggccgagca gggcggttcc tggggcgtga
     4621 gcgtcgtcgg gggtcctgac gaggtccggg cggccgtcga cgccgcccag cagtacaccg
     4681 tcgcggcccc gcacggcctg tcgctcgacc cccgcgtcat cgtgcaggag tacgtcgacg
     4741 gccccgagta cagcgccgac acggtcgtcc aggacggccg gccgttcccg ttgccggtgg
     4801 tccgcaagca caccaccgtg gacgcgtatc ggatcgagat cgggcacacc tgccccgcac
     4861 cgctccccca cgccgccgcg cacgagatcc aggccgtcgc cgcccgcgcc gcgctcgcgg
     4921 tggggatacg caacggcgtc gcccacaccg aactgaaggt tccccccggg agcggccgcc
     4981 ccgtcgtcat cgagacgggg gcacggctgc ccggcgggaa catctgcgaa gtggtggagc
     5041 acgccaccgg catcagcgag gcccgggcct atctgcgcgt ggtcctcggc caggccccgg
     5101 agacgacggc cctgcgggat gccgcctgcg cgatccgttt cctcgtcccg ccccgagcgg
     5161 gcattctgcg caccctgcgc accgccgacc tcccaggtgt gtacagcgag ttcaccgtac
     5221 gacccggcga tccgctgcgc gccccggtcg acaactcctg ccggatcggc catgtcatcg
     5281 cgacagcccc gaactccgcc caggccgaac tgctggccga ccgggcgata tcccagacga
     5341 ctatcgaggt ggcctgaccc gtgcacaaga tcgcgttcct gcgctccgtc gacatccagc
     5401 gcgccgaccc ctacatccag caggccgtcc gcgaactgac cgccgacggc atcgaatccc
     5461 ggctgtttta cacccacggc acgtgcggcc cggccgactt ccccgccggg ggtgagcacc
     5521 tcgaccccgc ggtgacaccc ctggaactgg ccgacgccgt gagcgcgtgg ggagcggaca
     5581 ccgccgtctc cgtctcgctg ccctgcgaga acgcattgcg cgacgcgacc gccaaagcat
     5641 tcctggacgc acgcggcatc cccaccgtga tgaccccgct ggccgccacc tggctgctct
     5701 gcgacaagtg ggagacgaag caggccctca tcgccgcagg actcgatgtc gcccgaggga
     5761 tccgggcgga cagcgatctg acggccgggc gcggtctgcc cgtgcccgcc taccgtcaga
     5821 gcctgctcgt cgccgccggt gagatcggct acccgctgct ctccaaaccg ctgtgggact
     5881 ccaccagcat gggcatccgc ttccttccgg accgcgccgc gctcgacgcc tatctcgccg
     5941 acccgccccc ggtcagcgcc gtgctggagg agtgtctgac cggcgagctg tgttccgtcg
     6001 acatcgtcgg cgcgcccggg aactacagcg tcctgccgtt gtgctggacc ggacaggcag
     6061 gcggcggggc cgcgttcacc ttcgccgacc tacgctggtg cggaccgcgc ccgcaggcgg
     6121 acgccgactt cgccccggtc gccgagcgtc tcgtcgggct gtgcgagagc ctcggcgtgt
     6181 acggctcggt caacgtcgac atgatccacg tcaacggccg ctacgtcgtc ctggagatca
     6241 atccgcgcat cggcggagcc accaccttgt cctgcgccgc ctccggcacc aacaccttcg
     6301 cgacgctcgc cgacatggca cgcggccggt acccggacgc cgcgcgaccc gacacccggc
     6361 gcaagcgctg ggcgatcgag ttcctggccg accggcccgt cggacaggcg gcggacgaac
     6421 tccgcgaccg cctcggcctg gtcaccgcgc acgagttggt catcgacggc accagccacg
     6481 gcgacatcgt caccttcacc tgtgaggaag gtgacgagca gcgcaccgtc aaggaactcg
     6541 acacgatcaa ctcccactcg ggcatcctcg cccccgtcat gatgggcaag gtccgcgctc
     6601 tgctgtgcgg cctgtgaccg gagcgaccgg ccgccgtgcg ggccgcggcc gggccgcccg
     6661 gatcgccggt gtcgccggtc tgcccgagct gcgcggacga ggccggttcg tcgtcgccca
     6721 cgtcatcgac tccatcggca acgggctgct gctgccgctg gggctgctgt tcttcatcac
     6781 cgtccggcac atcccggccg cgacggcggg cgcggcggcg agcacgggcc agttggccgc
     6841 cctcccgctc accgccctcg cgggccggat catggaccgc gccgggccga agtacctcgc
     6901 cgtcgcctcc aatctggtct gcgcggcggg cttcggcggc tttctcgtcg cggaacggcc
     6961 gtggcagatc gcggccgcgt acttcacggt ccaggcgggc atcaacggct actacaccag
     7021 ccagcggtcg ctgaccctgc tggtggcggc gggggacgaa ccgcgtacct ggttcgcgtt
     7081 cacctcctcg ctgcgcaaca cgggtctcgc cgccggagcc gcgatcgccg ccgcggccct
     7141 cgccctgtgg ggcaccggag cactgaccgc tctggtggtc gccgacgcgg cgagctactg
     7201 cctcgccgcc accttgttcg cgaccctcaa cgtggcgtcc ggacgcccca acgcacctcc
     7261 tggacgcacg acatggtcca tcgaccgccg ctacctggcc ctggtcctgt gcaacctgcc
     7321 ctacgtgttc gcccaggcga tcctgtcggt cctgctcgcg ctctacgcca cgagcgctct
     7381 acggctgtcc gcctggtccg ccagcgtgct gctgctcgtc aacaccgtgg ccgtcgccct
     7441 caccgcgaca gcggtgaccg cacacctggc cccgcgcgcc gcccggcacg cggtctccgt
     7501 cgcctacgcc gtgctcgccc tgtcgatggc cgcgttcgcc gcaccggccc tgccgggcct
     7561 gcgcccactg gcctggcccg cgctggcggt cggcatcgtg ctgttcaccg tcggggagat
     7621 cctctgctca ccggccatgg cggaactgtc caccacgctc ggtccggcgg ccgcccgggg
     7681 atcccaccag gcgctgttcc agatgtcgtg gtccgtcggc ggcgcggcgg cgcccgtgct
     7741 cttcaccgtg ctgctgcacc gttcgtcgct gttgccgtgg gccttcgagg cgatcctctg
     7801 cgtcctcgcc gccctggccg tcctcccgtt ggtcccctcg gggtcagcgt tcccgtccag
     7861 ccgcccccgt cgaaagcgca ctcggtgaac ctcgaatccg tccccacgct ggccgccgtc
     7921 cccgccgccg agtgggacca cctcgcccgc gacacgagct tctacctctc ccaccgctgg
     7981 ctggcgggcg aggagaccga ccccagcgcg acctgcacct acctcctcgt ccgggacccg
     8041 cacggccggc tgaccgccgc cgcccccctg tacctggtgc acgacgagcc caataccaac
     8101 taccggccgg agcaccatct ggacgtgggg caggacgaag ctccgcgcgt catcgccgga
     8161 gccaggcgcg ggtaccacaa cgcccctttg ctggcccccg ggttggacga ccggcaacgt
     8221 gccggctgcc tgacgatgct gcgcgacgcc gcacgtgagc acgccgcccg gcagagcacc
     8281 tggcactggt ggccctacct caccgaccgg gccgccacgg aactcggcga tgcctatggc
     8341 acggcgccgc gccggatcga ggacgacgcc accatctcgc tgcccggcac gggcttcgac
     8401 gactacctcg ccgcgctccc ggccaagcgg cgcagcggca tccgcggcga acgccgcgcg
     8461 ttcgacgccc gggccctgac cgtgcgcacc gaaccgctcg cgaactgcgt ggacgacgcc
     8521 ggacggctgc tggcgatcct gcagtgccgc cacggccatg tcaccgacac cgcggccatg
     8581 accggcgtca tgcgccgcca ggcccaggcc atgggcgagg acgcccaggt caccgcggcc
     8641 tacgacggcg cgaccatggt cgccttctcc ctcgtctacc acttcgggaa caccgcgtgg
     8701 ctgcgggccg tcggccatga tccccagcac accggggacg cgttcgagta cttcaacctc
     8761 gcctactacc tgccgatcga ggacgcgtac cggcacggct gcacacatct ccacgccggg
     8821 atgaagtccc tggccgccaa gcgcgcccgt ggagcacaca cgtcgccgct gtgggcgctg
     8881 gccgacccgc cacgctgacc ggggatccct ggacgcggct gtccgtacgg aaggacctct
     8941 gccggaccgt cgcgcgacgg tccggcagag gggcagtgag ggcctgagtg atcagacgcg
     9001 gtcggcggcg atgaggaggt actggaaact gccgtccttg taggcggtga ggaacgggtc
     9061 ctcgatgccg gtgaccagcg acgacttggc gcgcagctcc cagtacggga tcgtcgcgga
     9121 ggtgaggtcc acgacgctga tcgggacgag gcggttggcc gccatctcct tgaagtacgt
     9181 gctgcggggg tggatgtcgc agatgtagtg cgcgttgatc tgactcaccg cccgggaggg
     9241 cagtccatag gtgtcgttgt agcagccggt gatggtgacg taccgcccgc cgcgtgccag
     9301 cagccgggag tgctcggcga acagctgcgc cagatcgacg tacatggtgc tctcgttgtt
     9361 ccagatcccg cggaaggtac ccgagtcgaa gccggtgctc agcatgttct tgaggtggaa
     9421 ccgcaccttg tcgtccacac cccgcttgac ggcctggtcg ttggcgaagt cgacctggga
     9481 ctgggagatg gacaccccgt cgacggagca cccgaaccgc tggttcgcca cgatgctgcc
     9541 cccgccgcgg cccgatccgg cgtccagcag ccggtcctcg ggaccgatcg cgccgaactg
     9601 gtcgagcagg aactcggtct ggttgcactc cagtcggtgg agttccttgg tgaccaggtt
     9661 ctcgcgcatg tcctccgacg cgtcgaggac cgaccagtcg acgtcaccga tcccgtagtg
     9721 gtggtgatag atgccgtcca cgtcgcccag gcgcaggttg accgggttct tctcagcgtt
     9781 ccagtaggcg gcgacggacc gctggtaagg gctggtgttg aggagagggg cgggggcggt
     9841 gatgtggtcg atggtcatgt gcgggcttgc tcctttgatc gtcaggtcac ttcaggggct
     9901 gagctcaagg tctgggctca gcggctcggc tcaggggccg ggcaggctgg gcaggacgca
     9961 tgcgggagtg cggcgtcgtt cgtcggggcg ggttccgctc tcagggcgag gtggcatacc
    10021 ggccgccgac gtggccggcg tgccattcgt ggttgccgcc catccagttc gagagcccgg
    10081 ccacgtaccg ggcggcgacc ggatgagccg tgagcgcggc ggcctcggtc tcgaacgcgc
    10141 gcaccagctc gttgtgcact tcgatggcct tcgtgaaggc gtcctcgatc gcgcagtcct
    10201 cctcctccgc gatgacggtc ggcaggctgt agtggaagcg gcccgacgcc tgttccttgc
    10261 gcagggagta caggtcgttg aggaccgtgg acgcggtgga ggcgagcgcg gtcgcgcgct
    10321 gcacggacgg cagggcgaac agctccgcgg gcagttcgta accgccgatc gcgtccgtga
    10381 tcgccaggca cgggcggaag gagttgagct ggcgctgcac caggtactcc catacccggg
    10441 gggtgcgttc ctcctggacg caggcggctt cggcctcgta gccgaggtac aggctggcga
    10501 tctcatggcg gtaccggtgg gactgtgcgg gggtggcgag ctgggtgaag gactccatgg
    10561 ccgagtgcat ggcgcgcagc gccggatgcg cggccagggc cgcccgccag tcggcgtcgt
    10621 agggggcggg catgtggctg gggtcgatcg cggactgggc gaccgcgaga cgggcgccga
    10681 gaccgtcggg tgtgccgccc aactcgggtt cgcagaagta gtcgtccacc gcgttctcgc
    10741 cggccaggag cttggcggcg gtcagaaggt ggccggtggt gggggcgtcg gggtggcaca
    10801 gcacgacgga acggcccggg tcgaacttca gcagaccggc gcgcaggtcc ggggaccgga
    10861 acaggtccac gtcgtcggcc cattggagaa gttgctcatt gatcaggtcg gcgagcgttc
    10921 ggtcgtcgcg gaccgcgggc gggcagtgca ggccctcgat ccgcgcaggt gcaggtgccg
    10981 acgtaggcgt tggggccggg gccagtgcgg gtggctgtgc cgtcgccggt gccgggtggg
    11041 gttggcgccg ttgccggtgg ctgtgccggg cgggccgggg ggtggggcgg acgtgcgaag
    11101 cggctggcgg cgcttcccag accggtcggc ccggcgagga ctcgttccag cgcttcggcc
    11161 gggggctgac cccggccggt ggtggtcatt gcgtggctct ccctgcctga cgtggcgata
    11221 ggttcgatgc cgttcacgcg ccacgccgga atccttcgcg gtaacccaat gaataagttg
    11281 attctgagcc atgtgggcgc agcgcctggg ctgaatcatt accgtcatgg ctgcatctga
    11341 cactcactcg atcgagtgat agcgcggcgg aggaagatca gcaatacttc aaccggcgag
    11401 ttcccctgca atcaagagag ttgggccgaa gacggcacca ggtcggccgc cggccccggc
    11461 gacaggaatt cctccgagtc accatggcat acgcattcag gaactgacgc cgtacttcgt
    11521 gtggaaaagc aatccacgga cataaaagaa cgccacggca aaggactcca tccatgtcga
    11581 tcgaagcccg tccggaatcc gcgcccgctc agggaaacag cccttcccat acgagccttg
    11641 atacggctgc tgcccggaat ctggcgacca ccaccaagtc cgcacctcag atggcgggaa
    11701 tctcctcccg ctggctgctg cgcatgctgc cctgggtaga agtcacggcc ggcacctacc
    11761 gggtcaaccg ccggctgacc tactccgtcg gcgacggcaa ggtgaccttc gtcaaaaccg
    11821 gatcacaagt acgggtcgtc cccgccgaac tcggcgaact cgccctgctg cgctccttcg
    11881 aggacaccgc ggtcctcacc gcgctcgccg accgcttcac ccagcgcgag ttcgcccccg
    11941 gcgagctcct ggccgaagcc ggccagcccg tcgacaccct gttcctcctc gcccacggca
    12001 aggtcgaaaa gctcagcgag ggcgaattcg gcgagcagaa tcgtctcggc gtcctcgccg
    12061 acggcgccca cttcggcgaa cagggcctgc tcgacccgga aacaccctgg gacttcaccg
    12121 cccgcgccgc gaccaccacc accgtgctgg cactgtcccg acaggacttc cagaccctcg
    12181 ccgaccagtc ccccgccctg cgcgcccaca tcgaggccta ccgcgccatc ccccgccaac
    12241 gcgtcaacaa gcgcggcgag gccgccatcg aactggcctc cggacacaag ggcgaagcgg
    12301 acctgccgag cacgttcgtc gactacgaag tcgcaccccg cgaatacgag ctgagcgtcg
    12361 cccagaccgt actgcgcgtc cacagccgcg tcgcggacct ctacaacgaa ccgatgaacc
    12421 agatcgagca gcagctcaaa ctcaccatcg aagccctgcg cgaacgccag gaacacgaac
    12481 tcatcaacaa ccgcgagttc ggactgctgc acgcggccga ctacgaccag cggatccagc
    12541 cgcacgccgg ccccccgctc cccgacgacc tcgacgaact catcagccgc cgccgcggca
    12601 cccgcttcct gctcgcccac ccccgcacca tcgccgccat cggccgcgaa tggacccgca
    12661 aaggcctcta ccccggcaac gccgaagtcg acggcagcag cgtgctcgcc tggcgcggcg
    12721 tgcccatcct cagctgcgga aagatcccga tcagtgaggc ccgcaccagc tcgatcatcg
    12781 cggtgcgtac cggggaggac aaccagggcg tcatcggcct gcgccagacc ggcatccccg
    12841 acgagtacga acccggactc aatgtgcggt tcatggggct gagcgagaag gccatcatca
    12901 gctacctcgt cagcacctac tactccgccg cggtactcgt ccccgacgcc ctcggcatcc
    12961 tggagaacgt caacatcgcc aactggcgct gaggactgtc ccggtggccg ggcccgcccg
    13021 tcacacgggc ccggtcacgc ctccggtacc gagaagcaga ggagtttgcc tgagctgctc
    13081 acgtagaggt ggtcgtcgac ggggaacgcc gcccagtagc cggatccgcc gctgaccggg
    13141 tagtgccaga cctgttttcc ggtgccggcg tggaaaccgt agaggcctcc cggtgccgcg
    13201 gcgaggaaga ggccgcgtgc ggagcccagg aactggagtg tgccgaggcc ggagcccgcg
    13261 gtccagcggg gtgctccggt cagcgcgtcg acggcgtgca gggtcgagtc gaggtagcag
    13321 acggtcgcgc cggaccggtc gtagccccgg tttccgcggg cggccgtctg cacactccac
    13381 agggtctttc ccgtcgacgg gtcgaaggcc gcgagcagtc cgctgaccgc tcccgaggca
    13441 tcggtgcccg ggtagcagta cagagttccc tggtgcagct ccaaggaccc gtcataggcg
    13501 aggatttggc tgttcgtggg cggcaggctg gtggcgcggc tccagagggt ggcgccgtcg
    13561 gcgggcttga gggcgagcag gttcatggcg ccgtcgaggg cgtagagcac atcgccctcg
    13621 gacggcgggt agagggcgac gaggtccttc gcccacagcc gggcgcctgt gtccggagcg
    13681 acggtggtgt atccgcggag actcgcggtc gtggacgtct gcgaggtgct gccccagagg
    13741 accaggccgc ccgcccgggt ggtgccggtg accgcgatgc cgtcaccttc cggcgacttc
    13801 caccgctcgg cgccggtcga cgcgtcgagg ccctggaagt gcccgctgtc gaccatggcc
    13861 acgagcagcg aaccattggc gaactccagg tgcggtgcgc cggtcgcggt cgcgggccgt
    13921 tcccacagct gtttcccgga ccggacgtcg agcgcgtgga tctttccgtc ggtccgtacc
    13981 gtgtagacgt gtctgtcgtc cgcgatggcg gccgtgttct ggccgctcag gtccttccag
    14041 agcgcgttgc cggtgctccg gtcgtagcag gcgcccgagg tcttcccggc gcagagcagc
    14101 gcggacggcg tgaccgcccg cagggccatg tcgggctgcg ggaggtccgc gctccaggac
    14161 agttcgggag tggcggtcgc gccccggccg cctgagcgga gcagcagcgc ggtgcctccg
    14221 gcggccgcgg ccacggcgag caccccgccg ccggccagca gccggcgcct gctgaacagg
    14281 ggcgccctca ccgcttcgtg gaggtaccgc tccgacgcgt tcacctgcag ctcgaccgcg
    14341 ccgagccagc ccgcggtggt caccgtacgc agccacacct gcagttgccc gacgggtggg
    14401 cgccgggccg ggtccttgtc gaggcaggcg gtgaggatgc cgagcagtgc gtagggcacg
    14461 ccctccagcc gtggttcctc gtgggatgtg cggtagagca gcgcgtgcgc cgcgccggta
    14521 ccgaagggcg gggctccggt accggcgaag gccagcacgc tgccgagcga gaacacatcg
    14581 ctggcgggcg tgagttcggc tcccgtcgac tgctccggcg acatgtagcc gggcgacccg
    14641 atgatctggc cgtccgcggt gagcacggtc ccgtcgacgg cccggctgat gccgaagtcg
    14701 atgacccggg gcccgtcgcg ggccagcagg atgttggacg gcttgaggtc gcggtggatc
    14761 agccccacgc ggtggatggc ggccagcgcc tcggccagcc cggcgccgag cacgcgcagg
    14821 gtggcttccg gcatgggccc atgggcctgg atcgcttcgc tgagcgaggg gccggggatg
    14881 tactcggtgg ccagccaggg ctgcggtcct tcggggtcgg cgtcgacgac gggcgcggtg
    14941 aaggcgccgc cgaccgcgcg ggcggcgtcc acctctgccg tgaagcgccg ccggaagccg
    15001 ggatcggagg tcagctcggg acggacgacc ttgacggcga cggcgcgccc gctgcgcgag
    15061 gcggccaggt acacccggcc catgccgccc gcgccgagtt cccggatcac ccggtacggc
    15121 ccgatgtgcg gggcggagcc gggggccggg gtggccggcg tggccatcgg gctggtcatg
    15181 aggtgtcgct ccggaacgcg tagagggtgg tgtcggacgc ggcgtagacg gtgctgccga
    15241 cggcctgcag caaccacgcg ttcttgctcg cggattcggc gccggagggc tggtgcgccc
    15301 agagcagctt gccgtcggag gcgcgcagga cgaggaagcc cggggtgtcg cctgcggcga
    15361 acagcgggac cgccaccagg gacgtggaga cggcggggcc cgggttcagc gagttctgcg
    15421 ggtcgagggt cagcggactc gcggtgtgcc acacctggtc gccgctgccc gtgtcgaggg
    15481 cgaagacggt gccctggccc tcggcgacca cgtaggcccg ctgtcccagg accgtggtgg
    15541 ggttgagcgt ggtcgtcgag ttgactgcgg tccacaggcg gtggccgtcc gaagcgcgga
    15601 acgcctgcac cttgacgctg gtgaggagca gtgccttacc gtcgctggcc aggcccgggt
    15661 agtcgccggg cctgacgacg tcacggaccg tccaccgcac cgcgccgctc cgcagatcca
    15721 ctccgtggac gtcactgccg tcgtgcagaa agcagccgac cccgtcggag taggcgtgga
    15781 agtcgctgcc cttcaccttg tgggaccaca gcagcttccc ggtgacgagg tccacggcgc
    15841 gcacctggta ggccctgtcg ccgctggttc cgatcagggc gaccgagcgg gcgacgaaga
    15901 agacgtcgtg aatggagtcg gcgtaggagt ccccgtcctc cggcccggtg accgcgtggg
    15961 tgaaccgcag ggtgccgccg ccgtccacgc cgagcagatg gccgcgctcg ccgccccagg
    16021 ccgaggcgaa cagcgtcgat ccccgcacgc cgagccactc gagcttgccg gagacgtcgg
    16081 acggcacccg ggggtcggcc gtccagcgct tctgtccggt cgcggagtcg tagccggtcg
    16141 cggtggtctt gtcccagtgc acgagggtgc ccccgaggag ctccaggtgc gcgccggcct
    16201 gcagttgctg cagcggcatc gaccactgcg gcttcggtcc cgccgccacc ttggcgccgg
    16261 gccggatcgt ggtgcccgcg ctgccggggg cgccgcccgg ggagcggccg ggcttcgacc
    16321 tccggtcgat cagggccgtg gcgctcgcgg cggccacggc ggtgccgccg gcggcgatcc
    16381 acaggaagcg gcggcggctc ggcccgcccg gggccgccgt gtccggcggg ccgacgggga
    16441 tcggaggcac ggccagcagg gcggcggcgc tcgcttcccg tgcggcgatg tccgccagca
    16501 ggcccggggt gagcagtgag gagggttcga ccggacccag tgcggtcagc agcgcggtga
    16561 cggacggcct gcgggcgggc tccttgtcca gacaggccgc gaccagggga cgcagagcgt
    16621 cgggtacccc gtccagtcgc gccggggcgt ggacggcccg gtagaggatc ccgggcgtgc
    16681 tcccggtgcc gaagggaccc ccgccggtgg ccgcgaacgc caggacgcag ccgagggaga
    16741 agacatcggc cgccggtccg gtctcgcggc tcgacgcgat ctgctccgga gccatgaatc
    16801 cgggcgttcc catcacagcg ttcgtccggg tcacctgggt cgcctccgcc acccggctga
    16861 tcccgaagtc gatgacacgg ggcccgtcgt gggccaggag cacgttcccc ggtttgaggt
    16921 cgcggtggac gatgccggcg gcgtggatcg tcagcagcgc ctcggcgagt ccggcgccga
    16981 gggtccgcag ggccggctcg ggcagcgggc cgaagcgcgc cacggcgtcg tgcagggacg
    17041 gcgcggggat gtaggcggtg gccagccacg gctgctcggc gcccgcgtcg gcgtcgagca
    17101 ccggcggggt gaagaacccg ctcaccttcc gggcggcgtc ggcctcgcgc cggaagcgcc
    17161 cgcggaagtc cggcgcctcg gtgtactcgg gccggatcac cttcagcgcg accagccgcg
    17221 atcccggcga cctggccagg aagaccctgc ccataccgcc ctcgcccagg agttggagga
    17281 cctcgtaggg gccgatccgg cctgggctct gcgactgcgg ttgccgctgt tcctgcccct
    17341 gttcctggat ggtggtgcgc tgtcgcgggg cggtggggtc gttggcgccg tacgccggtg
    17401 tctgcacggt gtcgtgacgg cgggtcagct gagggagccg agtacgtcgc gggcggcggc
    17461 gacggcggcg tcctcggact gttgctggcg tgggccggag tccgaggtgt catagccgaa
    17521 actcacggag aacgcggcgt tgccctcgtg gacgatcacc gttgcctctg ccaggccccc
    17581 catgtccttg tcgacggcgt ggacgacgta ggcctcttcg ccgagaccgg tgagcgaccg
    17641 ggaatcggtg gtcgtgcgca tgtccttcag ggcgttgaga gcggtcgact tgtccatgtc
    17701 gtacatgccc ttcgctccgg ggctgaccgt gaggtgcacc cgcagatgca gcagccggcc
    17761 gccggaaccg gagggagtgt cccagctcgg catcttggtg atcatcgctg ccatggtcgt
    17821 gttgtcgaac tgcgacggct tgcactcggt tccgggcgcg atgctcgcga tggtggcctc
    17881 cttgatcagc cggcacccat cggggacgtg ggtgaacgtc cgtcccgccg gggcctgcga
    17941 cggaagcttg ccgccgcccg gggcaccgga cggactcccc gacgcggcgt tcttgtcatc
    18001 gcccccggat gtggcgatga tcacgccgac gatcgcggcc acgacgagca cggcgccgcc
    18061 gatcagccag ggcttcccac tcccgcccgg ccgccccccg ttcggcggtc cgtactgcgg
    18121 cggggtcggc gggaggtgct gcgaccaggc cgagggcgcg cccggcggct gggccgggac
    18181 cggcggcggc agctgcccgg gggctgcgcc gccggtcccc agcccggccc cccccggcat
    18241 cccctgccct cccggcatcc cctgccctgc cggcgtcccc tgcccgagcg gctgggtggg
    18301 gtcggtccgg taagggccct gccgggggtc catggggttc tgcggggtct cactcacggg
    18361 gcagttcgct ttccgtccgg ttctcggcat agggaacggc gcaggccagt cccgtgggcc
    18421 gggtccggga cgaaggcgca ccggtacccc acgcacttgc caaagtcttc cgcgaggcgc
    18481 tgcaatgctg atgaaatctt gctgaatcgg gggacgcgga ggactacggg gtgggcgcag
    18541 attcaacggg gtggctacag atcgacctgc tcgggccggt cagagccacc aggggcgacg
    18601 caccgctcga actcggtccg gtacggcggc aggaagtgct ggccgcgctg gtcctgcggc
    18661 cgggcaccct cgtcacccac cagcagctgc tcgacgacgt ctggggggcc gatcctccgg
    18721 gaacgggcca tcgggtcctg ccgagttacg tctatccgct gcggaagctg ctggacacgc
    18781 gggatgccgg tcctgccgag tcgttgatcc gcagcgagcg cggcggctac cgcttcgtgg
    18841 ccggcggggc acggagggac gtcgacgaac tggccgagcg ggccggcggc gcccggcgcg
    18901 cgcaggccgc cggcgatccc gcggccgccc tcgacaactt cgccctggct ctcgggttgt
    18961 tccgcggcga gccgctgacc ggtctgctgg ggccgttcgc ggacgccgaa cggcagcggc
    19021 tggcgcagca ccggcgcacg ctcgaacggg agcggctgga gtgtctggtg ctgctcggcc
    19081 ggtacacgga cgccctggac ggcctcgtgg tgccggccgc ggcccggccg tacgacgaac
    19141 cgctcgccgc gctgcggatg cgcgcgctgt acggcagcga ccggcaggtc gaggcgctgg
    19201 ccgtctacgg ggagacacgg gaccggctcc tcgacgagct gggggtggag cccggcgagg
    19261 aactgcgtcg ggtgcaccag ggcgtcctgc gccgggacga cgggctgctg ctcggccggg
    19321 tggtgcggca gcgcgcggcc gggtcggcgc ccgcgcgccc cgcgccgcag cggaacgaac
    19381 tgcccgggga caccgcctgc ctggtcggcc gcgaggccga actcgccctg ctcaccgcgc
    19441 ccgcgtccgc cggggccgtg tccgtggcgg ccgtggacgg cacggcgggt gtcggcaaga
    19501 ccgcgctggt ggtccgcgcc gcctggacgc tccgcgacca gtacccggac ggctgcctgt
    19561 tcgtggacct gcacgcgtac ggcaccggcc aggagagcac aggaccgcag cggacgctgc
    19621 accggctgct gcgcgcggtc gggagcgccg acggtgaact gccggacgag ctggacgagc
    19681 tggtgacggc ctggcgtgcg gcgacgagtt cgctgcggct gctgctggtc gtggacgacg
    19741 ctcccagcgc cgagctggtg cgcccgctgc tgcccgccgg gccgggcagc agggtgctgg
    19801 tggtgggccg gcagcggctg tccgggctcg acgccgacct gcggctcacc gtggagccgc
    19861 tcggcacggg cgagggggtc gcgctgctcc ggcacctcct cggtgaggcg cgcgcggacc
    19921 gggagccgga ggccgcgcgg gaactcgccc ggcgctgcgg cggtctgccg ctggcgctgc
    19981 ggatcaccgg ggcccggctg cagaaccgcc ggtcctggac gctggcccac ctggtggacc
    20041 ggctgtcgga cgacgagcgc cgcctcggtg aactgcgggc cgaggaccgc agcgtggagg
    20101 cggccttccg gatgtcctac gaccagctcg cgcccgggct gcagcgcggt ttccgcgcgc
    20161 tgggactgtc gccgacggcc gggttcgacg cgctcacccc ggccgtcatg ctgggctgct
    20221 cccgcgagga cgccgaggac gtcctggagc aactggtcga cgcgagtctg ctgcagcagc
    20281 cgcggccggg ccgctaccgg ctgcacgacc tggtgcggga ccacacgctg cggctcgccg
    20341 ccgcggagcc cgaggagtcc gcggccgccc gctccgccgt gctgcacctc tacacggccg
    20401 ccggccggat cgccagcgac tggggccccg agggcttccc gacgggaccg gacgtcaccg
    20461 gctcgccgtt cgccgactgg caggccgccg ctgcctggtt ggacgccgcg gcctgccaac
    20521 tggtcgatgt ggtggcctgc gcggtgaccg ccggggaggc cgaccacgcc tgctggatcg
    20581 ccgagggcct ggtcgaccat ctggtccgcc aggggcgcta ccacgagtgc cggtcggccc
    20641 tggaactcgc gctgacctgc gcggacacgg ccgatgaccg gcggatgccc tcctccctgc
    20701 ggagctgtat gggcatcgtc gacatctacc aggggcggtt ccggcaggcg cacgcctggt
    20761 tcaccgacgc actgcgcatc gcccgccgcc tgggcgatct gcgggaacag gcgcgggcgc
    20821 tggccgggat gggcgcctcg gagcggtcgc tggaccgcac cggggaggcg gtcgcccacc
    20881 tgagtgaggt ggtggagctg gcggcccggc tcgacgacga ctggctgggc gggatgtcga
    20941 cctgcaacct cggcgccatc caccaccatc aggggcgcta cgaggacgcg ctcgcgtact
    21001 tcgccacctc cctcgcgttc gccgagaaga tcgaccggcc cagg
//
