LOCUS       NZ_SPGD01000010        12013 bp    DNA     linear   CON 20-MAY-2024
DEFINITION  Streptococcus anginosus strain 1001175st1_H11
            NODE_10_length_69994_cov_77.1519, whole genome shotgun sequence.
ACCESSION   NZ_SPGD01000010
VERSION     NZ_SPGD01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus anginosus
  ORGANISM  Streptococcus anginosus
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus; Streptococcus anginosus group.
REFERENCE   1  (bases 1 to 69994)
  AUTHORS   Yang,C., Faith,J. and Aggarwala,V.
  TITLE     Strain-level differences in gut microbiome composition determine
            fecal IgA levels and are modifiable by gut microbiota manipulation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 69994)
  AUTHORS   Yang,C., Faith,J. and Aggarwala,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2019) Genetics and Genomic Sciences, Icahn School
            of Medicine at Mount Sinai, 1470 Madison Ave, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 85.9707156971x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005844435.1-RS_2024_05_20
            Annotation Date                   :: 05/20/2024 01:50:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,133
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,937
            CDSs (with protein)               :: 1,937
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 73 of 151
            Pseudo Genes (incomplete)         :: 73 of 151
            Pseudo Genes (internal stop)      :: 52 of 151
            Pseudo Genes (multiple problems)  :: 40 of 151
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:21:34
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 18287
            Orig. end                         :: 30300
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            SPGD01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12013
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12013
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12013
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[<5000:7013](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<5001..7013)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    751..764
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.03"
                     /tool="antismash"
     gene            1020..1733
                     /locus_tag="FGQ50_RS05560"
     CDS             1020..1733
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009570335.1"
                     /locus_tag="FGQ50_RS05560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_024051975.1"
                     /sec_met_domain="Abi (E-value: 5.1e-15, bitscore: 47.7,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLACSFIFLIFFTILLAGFPETVALHIAILFKWNLDAVGVMVL
                     VQEILLLFFIIYLLKRANLYSIFKKKKIRRGDILGLLALCFIFYFLTHIRQELVQYMAR
                     TTMNAKLYSKVGIWTGSKLFEICSVLITMLISPIIEELFYRGYVMSRIFVKSKYYLDVL
                     FSASLFALGHMVLLQRDWVNLSFYFLSGFAMSLFYRYCKNIRLQIFFHISWNVYTFIAS
                     IWYIIYNWIYFHFFF"
     gene            1880..2619
                     /locus_tag="FGQ50_RS05565"
                     /pseudo=""
     CDS             1880..2619
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219140.1"
                     /locus_tag="FGQ50_RS05565"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /pseudo=""
                     /transl_table=11
                     /translation="MFILEDNIVQQYRIETIIKEILEEHHLQYHNFEVFGKPKQLLEAI
                     SEKGSHQVFFLDIEIKTEEKRA"
     gene            2653..3948
                     /locus_tag="FGQ50_RS05570"
     CDS             2653..3948
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037258.1"
                     /locus_tag="FGQ50_RS05570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_230318993.1"
                     /transl_table=11
                     /translation="MFLLLFLFSYVSGQKLTSKIYLVAILTNLLYSISFSAIPNSLFSY
                     LIGPTYSLIFAWIIGKSYSKTLKIFYGLFPITLWNLIQRLIIFFIFPLFGFGVVRLNKS
                     LLGPIMTSIMAALMVLPFLRLFQYNFKTLSFQLIDQKDKKVLIFVNLSMIIYHLIVQIL
                     AYLENESGAQTLVFRESLVVFYLILFMSAANYLDKRLRERIQSDLMFQKDLQLKNLKDY
                     SHHIEGLYTEVRSFRHDYANILTTLKLGIEQNDIEIVKNVYDSVLKDSNKHFRNPKYDI
                     GRLIHIKNDALKSLLAAKCAQAQEHNVAVSLEIPEDIYSQGMELVDFITIVSVLCDNAI
                     EAAAPNMTIAYVSSGQKQIFSVENSVKEESVDISQIFDLGVSSKGATRGVGLHNVISIL
                     DRYPNVSLNTTSQNYCFRQTIEIQIKGNHYCN"
     gene            complement(3970..4119)
                     /locus_tag="FGQ50_RS05575"
     CDS             complement(3970..4119)
                     /GO_function="GO:0005186 - pheromone activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037249.1"
                     /locus_tag="FGQ50_RS05575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="WP_022526763.1"
                     /transl_table=11
                     /translation="MDKEQTLNHFHTLTDQELEQIVGSGWLEKLLSPYLNKYKLGQSSQ
                     TNLG"
     gene            complement(4213..>5028)
                     /locus_tag="FGQ50_RS05580"
                     /pseudo=""
     CDS             complement(4213..>5028)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009570339.1"
                     /locus_tag="FGQ50_RS05580"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /pseudo=""
                     /transl_table=11
                     /translation="HEITRQNHYHELYNAISSNASNLPKSNPHQATFNLYKNEYHVKHD
                     SSTTNQYLAQIDTKISSLNSSIANLKIQKAGTGTATTYDNSLSTKIEVLRTQFIQTADQ
                     QLASLTTQITDLQNQLNQANAQLQNNQLTAPETGILHISSTLKGKTLLPKGTEIAQIYP
                     DITKTKEVLITYYVPSTYMTNLKKGQTSRLTLEKIGNQTITIIGKINSIDTSATETKQE
                     NLFKITAKASVSKKDSQILKYGLQGRVTSVIAKKSFFHYYKDKLLNNVE"
     gene            complement(<5001..7013)
                     /locus_tag="FGQ50_RS05585"
                     /pseudo=""
     CDS             complement(<5001..7013)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768646.1"
                     /locus_tag="FGQ50_RS05585"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="peptide cleavage/export ABC transporter"
                     /pseudo=""
                     /sec_met_domain="Peptidase_C39 (E-value: 4.4e-47, bitscore:
                     151.2, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1047.6,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNFKKSFVPQIDARDCGVAALASIAKYYGSNYSLAHLRELAKTNK
                     EGTTALGIVKAAKEMGFETRPIQADMTLFDTEELPYPFIAHVNKDGKLQHYYVVYKNEQ
                     KHLLIGDPDPTVKITKISKEQFTKEWTGVAIFLAPKPSYKPHKDKKNGLASFLPLIFKQ
                     RSLIFYIVLASLLVTLINIVGSYYLQSVLDEYIPNQMKSTLGIISIGLIIAYILQQMMS
                     FSRDYLLTVLSQRLTIDVILSYIRHIFELPMSFFAMRRTGEIISRFTDANSIIDALAST
                     ILSFFLDLFIVMIVGSVLLLQNVNLFLLTLLAIPIYALIIFVFMKPFEKMNNDVMQSNA
                     MVNSAIIEDINGIETIKSLTSEESRYQKIDSEFFDYLDKTFKLSKYSIMQTALKQGAQF
                     VLNVVILWVGASLVMSQKISIGQLITFNALLSYFTSPLESIINLQTKLQSAKVANHRLN
                     EVYLVESEFKTKQVLTEKNFLAGDITFHNVSYKYGFGRDTLSDISLTIKKGDKISLFGI
                     SGSGKTTLAKMIVHFYEPYQGQIRLNGNDLKTIDKKVLRQYINYLPQQAYIFSGSILDN
                     LTLGADQTVSQSDILRACEIAEIRSDIEQMPMGYQTELSDGAGLSGGQKQRIALARALL
                     TKSPILILDEATSGLDVLTEKKVIDNLMKLPDKTII"
     misc_feature    7175..7193
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 24.88"
                     /tool="antismash"
     gene            7295..8350
                     /locus_tag="FGQ50_RS05590"
     CDS             7295..8350
                     /GO_function="GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /GO_process="GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897971.1"
                     /locus_tag="FGQ50_RS05590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="serine hydrolase"
                     /protein_id="WP_024051969.1"
                     /transl_table=11
                     /translation="MSKKTIILCLLGCFMMCGVLVLSAVHFGQARKPHHTKVAKSYTVS
                     QEKKKSPSKESKPATRVPIKSMEEKQKVMEADLPVMGAYGLYYDYANLNLVQVVQAYLD
                     EMGIEHSQVAFSYKDLTSGQTYAMNETQPMTAGSTYKLPLNMLVVDEVEKGNLSLTERF
                     DITHTRYEYIGEHNNYVAAFNGTMNIPEMQEYSLLYSENTPAYALAERLGGLDKAYTMY
                     QRYGKGKGEIKTINQQNQTTTDYYIQVLDYLWKHQEKYKEILYYIGESFPNEYYKRYVR
                     DIPIYQKPGYVAEALNIDAVVCEAKPYLIALYTAGLGGTTSESDEISGVGITQVGQLAY
                     VINEWHRVNMN"
     misc_feature    complement(8394..8408)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     strong, score: 24.54"
                     /tool="antismash"
     gene            8486..9172
                     /locus_tag="FGQ50_RS05595"
     CDS             8486..9172
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069910.1"
                     /locus_tag="FGQ50_RS05595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_024051968.1"
                     /transl_table=11
                     /translation="MINKEHYRYIREHPAFENLPVEHFDKIAVEIQFRKVPKGQIIFFS
                     GDKRERLFLIYKGYVRIEQYDQTDSFNYIDYVKENTMFPYGGMFEDKDYHYSGISVTDL
                     EYFSIPVDLYEYYSKQIIEQMLFITHKLSNILRFHELRLRNSVLASATERVIQVISILC
                     IDFCQDRDDIPFALSMKELAKLGATTRETVNQVLKKLTEENIIQYNRKKLVFLNKEYFL
                     KYFEDM"
     gene            9493..10722
                     /gene="arcA"
                     /locus_tag="FGQ50_RS05600"
     CDS             9493..10722
                     /EC_number="3.5.3.6"
                     /GO_function="GO:0016990 - arginine deiminase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="arcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /locus_tag="FGQ50_RS05600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="arginine deiminase"
                     /protein_id="WP_021001457.1"
                     /transl_table=11
                     /translation="MSTHPIHVFSEIGKLKKVMLHRPGKELENLMPDHLERLLFDDIPF
                     LEDAQKEHDAFADALRKEGIEVLYLEKLTAESLISPEIRNEFIEEYLDEANIRGAQTKV
                     AIRDILHGIEDNLELVEKTMAGFQKAELPEIPEEAKGLTDLVESDYPFAIDPMPNLYFT
                     RDPFATIGNAVSLNHMYSDTRNRETLYGKYIFKYHPVYGGNVDLVYNREETTRIEGGDE
                     LVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVIAFEFANNRKFMHLDTVFTMV
                     DYDKFTIHPEIEGDLRVYSVTYENEKLHIAEEKGDLAELLAKNLGIDKVHLIRCGGGNA
                     VAAAREQWNDGSNTLTIAPGVVVVYDRNVVTNKILEEYGLRLIKIRGSELVRGRGGPRC
                     MSMPFEREEL"
     gene            10746..11762
                     /gene="argF"
                     /locus_tag="FGQ50_RS05605"
     CDS             10746..11762
                     /EC_number="2.1.3.3"
                     /GO_function="GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA]"
                     /GO_process="GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /gene="argF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1114: ornithine carbamoyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /locus_tag="FGQ50_RS05605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ornithine carbamoyltransferase"
                     /protein_id="WP_003028184.1"
                     /transl_table=11
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLAAHLKDLKKRNIEHHYL
                     AGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMFD
                     GIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLVYCG
                     DGRNNVANSLLVTGAILGVNVHIFSPKELFPAEDIVKLAEGYAKESGAHVLITDNADEA
                     VKGADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLIKKADNEDLIFLHCLPAFHDTN
                     TVYGKDVAEKFGVEEMEVTDEVFRSKYARQFDQAENRMHTIKAVMAATLGNLYIPKV"
     misc_feature    11913..11926
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.53"
                     /tool="antismash"
ORIGIN
        1 ttcgtcaagc tgttatcaat aacggtggtg gtcatctcaa ccatgctctt ttctgggaat
       61 tgatgacacc agaaaagaca gagccatcag cagcattagc agcagacctt gaagcaacat
      121 ttggttcatt tgatgacttc aaagcagcat ttacaacagc tgcggcaagt cgctttggtt
      181 caggttgggc ttggctggtg gtcaataatg atggtaaatt agaagtgact tcaactgcta
      241 atcaagacac accaatctct gaagggaaaa caccaatttt aggaattgat gtttgggaac
      301 atgcttatta tgtgaagtac cgcaatgttc gtccagatta catcaaagca ttcttctcag
      361 tcatcaactg gaataaggtt aatgaattgt acgcagcagc aaaataaggt gtgcaatgag
      421 atagaaaaaa cgaatctgat taggttcgtt tttttctgtt atagtaaaat tacctataaa
      481 ttccttccaa ataaaaccag atttgttgtt tgaagaattt cagagaatga tcgtttgttc
      541 tgtcaatcac accaaaaacg agatagtgat ttttaaataa tagctcataa tcaatatttt
      601 ctccgacaat aataattttt cctcctgtag aattatctta ccatataata tgatgtatgg
      661 atgagcaaaa tcttaaatag tcgtcatatg tactgaacga tatttttgac cattcaggac
      721 atcagaatta ccattcagga ttttccggaa attttttata aaaagttgat aaaatgagaa
      781 tgtaacgtta caaaaataaa ttatagaaaa gaggaatatc tatgaatcat ttaacattta
      841 gataattgtc ggaacaagac ttagccaact ataacggtgg tggatacact tttgaagcat
      901 atcgtggtga gtttccgcca ccatcatatt atagccaagc attaagccca caagctttac
      961 acgcacgtat gactgcatgg gcatatggag ttcgtccata attaaaatca ggagacgtga
     1021 tgaagaaact agcatgttca tttatttttc taatattttt cactattttg ctagctggct
     1081 ttccggaaac agtagcgctt catattgcta ttttgtttaa gtggaattta gatgcagttg
     1141 gtgtgatggt gttggttcaa gagattttgt tgctattctt catcatttat ctcttgaaac
     1201 gagcaaattt atattctatt tttaaaaaga aaaagatccg tagaggagat atactagggt
     1261 tgcttgcttt gtgttttata ttttacttct taacgcatat tagacaagag ctggtgcagt
     1321 atatggctcg gacaaccatg aatgcaaaac tttatagtaa ggttggtatt tggactggga
     1381 gtaaactatt tgagatttgt agcgttttga taacaatgtt gatttctcca ataattgagg
     1441 aactctttta ccgaggttat gtcatgtctc gtatttttgt taagtcaaaa tattatttag
     1501 atgtcttatt ttcagctagt ctgtttgcat tggggcatat ggtcttactt caaagagatt
     1561 gggtcaatct cagcttttat ttcttaagtg ggtttgcaat gtctctattt tatcgttatt
     1621 gtaaaaatat acgactccag attttctttc atatatcatg gaatgtatat acttttatag
     1681 cttctatttg gtatatcatt tataattgga tttattttca tttctttttt taataggtga
     1741 aaacaagttg ataggtatta gcttgtttat ttttgtttaa ttattttgaa tatacaaaat
     1801 attctttcat tttatttttg tgaatttctt attcttttta tataataaat tatatgatag
     1861 ggagaatcat gaaaggaata tttttatatt agaagataac attgtgcaac aatatcgcat
     1921 tgaaacgatt attaaagaga ttctagaaga acatcatcta caatatcaca attttgaagt
     1981 ctttggaaaa cctaaacaat tactagaagc catatctgag aagggaagcc atcaggtttt
     2041 ctttttagat attgaaataa aaaccgaaga aaaaagggct tagatgttgc caaaaagata
     2101 cgcgaaatag ctccttatgc agtcatcgtg tttgtaacca gtcattctga attgatgcct
     2161 actgcttttc gactacaggt tgctgctctt gattatatcg acaaatcttt atccaataaa
     2221 gcctttaaag cacggataga aactgcgttg tttcacgcta atagtatgac gggaaaagga
     2281 ctggcggacg actcctttat ttttgagtct cctaatgcac agattcaagt tccatttaaa
     2341 gacatccttt atatagaaac ttcgtctcgc cctaatcgct tagtccttta cacatataga
     2401 ggacggacag aatttactgc taatctttca gatattttag agcaagaaaa acggctatat
     2461 aaatgtcacc gttcctttgt tgttaaccct gcgaacattg ctcgaataga gaaaaaagaa
     2521 cgtatccttt atttcccaaa cggtgctact tgccttattg caagaacaaa attaaagggg
     2581 ttgttagaag tagttgcagc cctacatcgg aggagatgat agataataca actattatta
     2641 gtagttttag atatgtttct gttattgttt cttttttctt atgttagtgg ccaaaaatta
     2701 acaagtaaaa tatatctagt agcaatttta acaaatttac tttatagtat aagtttttct
     2761 gctatcccaa atagtttatt ttcgtactta ataggtccga cctattcctt gatatttgct
     2821 tggattattg gaaagtctta tagtaaaaca ttaaagattt tttatgggct ttttccgatt
     2881 acgttatgga atttgataca acgtttgata atctttttta tatttccctt atttggattt
     2941 ggtgtggtaa gacttaataa aagtttgttg gggccgatta tgacatcgat tatggctgct
     3001 ttaatggtat tgcctttttt acgattgttt caatataatt ttaagacatt atcatttcaa
     3061 ttgattgacc aaaaagataa aaaagtttta atctttgtaa atttgagtat gataatttac
     3121 catttaattg ttcaaatctt ggcttattta gagaatgaga gtggtgcaca aacattagtt
     3181 tttagagaaa gtttggtagt cttttatctt atccttttca tgagtgctgc aaattatttg
     3241 gataagcgtt tacgagaaag gattcaaagt gatttaatgt tccaaaaaga tttgcagtta
     3301 aaaaatttga aagactacag tcatcatatt gagggattat atacagaagt tcggagtttt
     3361 cggcatgatt acgcaaatat tttaacgact ttgaaattag gaattgagca aaatgatata
     3421 gaaattgtaa aaaatgttta tgactcagtg ttaaaagatt ccaataagca ctttcgtaat
     3481 ccaaagtatg atattggacg attaatccat atcaaaaatg atgctttaaa gagcctgtta
     3541 gcagctaagt gtgcacaagc tcaagaacat aatgtggcag tttcattgga gattccagaa
     3601 gatatttatt cacagggaat ggaattagtt gactttatca ctattgtctc cgttttgtgc
     3661 gataatgcta ttgaagctgc tgctccaaat atgacgattg cctatgtttc atcaggacaa
     3721 aagcagatat tttccgttga aaattcagta aaagaagaaa gtgtggatat ttcacaaatc
     3781 tttgacttgg gagttagttc gaaaggggct acgagaggag tgggactgca taatgtaata
     3841 tctattttgg atagatatcc taatgtttcg cttaatacaa ctagtcaaaa ttattgtttt
     3901 cgtcagacaa ttgagattca aataaaagga aatcattact gtaattaaat tcagtaatga
     3961 ttttgtatat tatcctaaat tagtttggct ggattgacct aatttatatt tatttaagta
     4021 aggagaaagt agcttctcta accagccact accaacaatt tgttcaagtt cttggtctgt
     4081 gagagtatga aagtgattaa gtgtttgttc tttgtccata ataggaaccc cctttttgat
     4141 aaatttatta taaactaaat tttgctttgt gtatcattgg atcttaaacg gaattgattt
     4201 cgtcttaaac gatcattcaa cgttgtttaa cagtttatct ttgtagtaat gaaagaatga
     4261 ttttttcgca atgacactcg ttactcgtcc ttgcaaaccg tacttcaaga tttggctatc
     4321 ttttttagat acggaggctt ttgcagtgat tttaaacagg ttttcctgtt ttgtttcagt
     4381 agcagaagtg tcaatgctgt taattttacc gataatagtg attgtctgat ttccaatttt
     4441 ttctagagta aggcgggaag tttgtccctt ttttaaattg gtcatatagg ttgaaggaac
     4501 gtaataggtg ataagaactt cttttgtttt cgtaatatct ggataaatct gagcaatttc
     4561 cgttcctttt ggtagtaaag tctttccttt gagcgtactg ctgatatgaa gaatacctgt
     4621 ttcaggagca gttaactgat tgttttgaag ctgagcattt gcttggttca attgattttg
     4681 caaatcagta atttgtgtcg taagacttgc taactgttgg tctgctgttt ggataaattg
     4741 tgtgcgaagt acctcaatct tagtcgaaag gctattatca taggtcgtcg cagttccagt
     4801 gccagctttt tgaatcttta gattggcaat ggaagaattt aggctggata tctttgtatc
     4861 aatttgagct aagtattgat ttgtagttga cgaatcatgt ttgacgtgat attcattttt
     4921 atataagtta aaggttgctt gatgaggatt gctttttggc aaatttgagg cattgctaga
     4981 aatggcatta tacaattcat gataatggtt ttgtcgggta atttcatgag attatcaatg
     5041 acttttttct ctgttaaaac atctaatcca ctcgttgctt catctaaaat gagaatggga
     5101 gatttcgtca gcagagcgcg tgcaagtgca attcgttgct tttgtccacc agataatcca
     5161 gctccgtcag acaattctgt ctggtatccc atgggcattt gttcgatatc tgaacgaatt
     5221 tctgcaattt cacaggcacg aagaatatct gattgagaaa ctgtttgatc ggctcctaat
     5281 gtgaggttgt ctagaatgga accactaaag atgtaagctt gctgaggtaa gtagttgata
     5341 tattgccgaa gaactttctt atcaattgtc ttgaggtcat ttccattgag gcgaatttgt
     5401 ccttgataag gctcataaaa atgaacaatc atcttagcaa gtgttgtttt tcccgaaccg
     5461 ctaataccga aaagactgat tttatctccc ttcttgatag ttaaactaat atctgataaa
     5521 gtatctcgtc caaagccata tttataggag acgttatgaa aagtaatatc accagctaag
     5581 aaatttttct ccgtaaggac ttgtttggtt ttgaattcgg attctaccag ataaacttca
     5641 ttcagtcgat gattggcaac tttagctgat tgcagtttgg tttgaagatt gataatactt
     5701 tcaagcgggc ttgtaaaata cgaaaggagt gcattaaaag taatcagttg accaattgaa
     5761 atcttttgag acatgactaa ggacgctcca acccagagaa tgaccacatt caaaacaaat
     5821 tgtgctcctt gtttgagagc ggtctgcata atggaatatt tacttaattt gaaagtttta
     5881 tccagataat caaaaaactc gctatcaatc ttctggtaac gactttcttc actggttaag
     5941 gatttaatag tttcaattcc attgatatct tcgataatgg cagaatttac catagcattg
     6001 ctttgcataa catcgttatt cattttttca aagggcttca taaaaacaaa aataatgaga
     6061 gcgtagatag gaattgcaag caaagttagt aaaaagagat tgacattttg cagtaaaaga
     6121 acactaccga caatcatcac aatgaacaaa tcaagaaaga aagacaggat agttgaagct
     6181 agagcatcaa taatagagtt ggcatctgta aaacgagaaa taatctctcc agtgcgtctc
     6241 atcgcaaaaa aggacattgg caattcaaaa atatgccgaa tataagacaa aatcacatca
     6301 attgttaatc gttgactgag tactgttagc aggtagtctc ttgaaaaact catcatttgc
     6361 tgtaagatgt aggcaatgat aagtccaatc gaaataatcc caagagttga tttcatctga
     6421 tttgggatgt attcgtccaa aacactttgc agataatagg agccaacaat gttgatgagg
     6481 gtcacgagca aactagctaa gacaatataa aaaatcaaag aacgctgttt aaagatgagt
     6541 ggtaaaaagc tagcaagtcc atttttctta tccttatgag gtttatagct aggttttggg
     6601 gcgagaaaga tagcaacacc tgtccattct ttagtaaatt gttctttgct aatcttagtt
     6661 attttgacag ttggatcagg atcaccgata agcaagtgct tttgctcgtt tttataaacg
     6721 acataatagt gttgaagctt gccgtctttg ttgacatgtg cgatgaaagg atagggaagc
     6781 tcttctgtat caaacaatgt catgtctgct tggattggtc gggtttcaaa gcccatttct
     6841 tttgctgctt tgacaattcc aagtgcagtt gtcccttctt tgtttgtctt tgcgagctct
     6901 ctcaagtgtg ctaaagaata atttgaacca tagtatttag caatggaagc cagagcagca
     6961 acaccacaat ctctagcatc tatttgtgga acaaacgatt ttttaaaatt cactataatg
     7021 ttttcctttc gttttgtaat gttattttct caaaatacgc aagaaaaaga gtattttaat
     7081 cttaaatggt agttctgatg tcttaaatgg ttaaaaaaca ctatttagga tgtgaaagct
     7141 accattcagg cttattttaa atgaactaga attgttactt tatactgtat atatacaata
     7201 aaggaagttg aaaataattg atgattctgt ttatcatatc ttgtcatttt ttcattcttc
     7261 ctatataatg aggtatataa gttaggagaa gtgcgtgagt aaaaagacaa taattctttg
     7321 tttgcttggt tgctttatga tgtgtggtgt gttggtgtta agtgctgtgc actttgggca
     7381 agctagaaag ccacatcata cgaaggtagc taaaagttac acagttagtc aagaaaagaa
     7441 aaaaagtccg tcaaaagagt caaaaccagc cactcgtgtt ccaataaaaa gtatggaaga
     7501 aaagcagaag gtcatggaag ctgatttgcc ggtaatgggt gcctatggtt tgtattatga
     7561 ttatgccaat ctaaatttgg ttcaagttgt acaggcttat cttgatgaaa tgggtattga
     7621 acattcacaa gttgcatttt cttataagga tttaacgagt ggacaaacct atgctatgaa
     7681 tgagacgcag ccgatgacag caggctcaac ttacaaattg ccattaaata tgttggtcgt
     7741 tgatgaggta gaaaagggaa atttatcact aaccgagcgg tttgatatta cacatactcg
     7801 ctatgaatat atcggtgaac acaataatta cgtagcggca tttaatggga caatgaacat
     7861 tcctgagatg caggaatact ctcttctcta ttctgaaaat acacctgctt atgctttagc
     7921 ggaacgctta ggtggcttgg ataaagccta tacgatgtat cagcgctatg gaaaaggaaa
     7981 gggcgagatt aagacaatca accaacagaa tcaaacaacg acggattatt atattcaggt
     8041 attggattat ctttggaaac atcaggagaa gtataaagag attttgtatt atatcggtga
     8101 atcttttcca aacgaatact ataaacgata tgtaagagat attccaatct accaaaagcc
     8161 aggttacgtt gcagaagccc tgaatataga cgcagttgta tgtgaagcaa agccttattt
     8221 gattgccctt tatacagcag gtctaggggg aacgacttcc gaaagcgatg agattagcgg
     8281 cgttggtatt acgcaagttg gacaattggc ctatgtcatc aatgaatggc atcgagtgaa
     8341 tatgaattaa gatgcatata tttatagggt gaaggacacg ttgtaaaaag aacaaatttc
     8401 agaaaaatgt aagggttctc tttaagattg aagccctttt gtgttataat aagaacagaa
     8461 tgtaaaaaag gatcacaagg atatcatgat taacaaagag cattatcgtt acattaggga
     8521 acatcctgct tttgaaaatt taccagttga acattttgat aaaatagcgg ttgaaattca
     8581 atttcgcaaa gtgccaaaag ggcaaattat tttcttttct ggagataagc gagagcgact
     8641 ttttttgatt tataagggtt atgttcggat tgagcagtat gatcagacag attcgtttaa
     8701 ttacattgat tatgtgaaag aaaataccat gtttccttat ggtggcatgt ttgaagataa
     8761 agattatcat tattctggga tttctgtgac ggatttagaa tacttttcga ttcctgttga
     8821 cttgtatgaa tactattcca aacaaattat agagcaaatg ttattcatta cgcataagtt
     8881 atcaaatatt ttgcgtttcc atgaattacg cttgcgaaat tccgttttag cgagtgcgac
     8941 agagcgagtt atccaagtga tttcaatttt atgtattgat ttttgtcaag atagagatga
     9001 tattcctttt gcccttagta tgaaagagtt agccaagttg ggggcgacca ctcgtgaaac
     9061 agttaatcaa gtgttgaaaa aattgacaga agaaaacatt attcaatata atcgaaagaa
     9121 actcgtcttt ttgaataaag aatacttttt aaagtatttt gaagatatgt agaaaagaag
     9181 gacagaattg cagtccaaaa agcaattttt ggtccttttt taatttagaa acctaaacat
     9241 ttaaaaatat cctcaaaaac ttcatttgtt tctaaaaaat aattattcat aaaatcaata
     9301 aaatgcataa aatatttata gaaagcgttt tattttgggg aaatatctga atttctttta
     9361 aaaaaagcaa aggatttggc attttaaatg tataattgat tgtgaaagcg aaaacaaaag
     9421 atattacaat atgacatgta aatagaactt aaggaaactt aagtcacaaa gaaaaaaaga
     9481 ggaggacata agatgtctac acatcctatt catgttttct cagaaatagg aaaactgaaa
     9541 aaagttatgc tacatcgtcc tggtaaagag ttggaaaact tgatgccaga ccatttggaa
     9601 cgtctacttt ttgacgatat tccattcttg gaagatgctc aaaaagaaca cgatgcattc
     9661 gcagacgctc tacgtaaaga aggtattgaa gttctttacc tcgaaaaatt gactgctgaa
     9721 tcattgattt ctccagaaat tcgtaacgaa tttattgaag aatatcttga cgaagcaaat
     9781 attcgcggag cacaaacaaa agttgctatt cgcgacattc ttcatggtat agaagataac
     9841 cttgagttgg ttgaaaagac aatggcaggc ttccaaaaag cagaattgcc agaaattcct
     9901 gaagaagcta aaggactgac tgacttggta gaatcagact atccatttgc cattgaccca
     9961 atgccaaacc tttatttcac acgtgaccca tttgctacaa ttggtaatgc ggtgtcattg
    10021 aaccacatgt actcagatac acgtaatcgt gaaacacttt atggtaaata cattttcaaa
    10081 taccacccag tatatggtgg caatgttgac ttggtttata accgtgaaga aacaactcgt
    10141 attgaaggtg gagatgaact cgttctttct aaagatgtcc ttgcagtagg tatttcacaa
    10201 cgtacagacg cagcatctat tgaaaaactt ttggttaata tcttcaagaa gaatgttggc
    10261 tttaagaaag tgattgcatt tgaatttgct aataatcgta aattcatgca cttagataca
    10321 gtcttcacaa tggttgacta tgacaaattc acgattcacc cagaaattga aggcgacctt
    10381 cgcgtttact ctgttactta tgaaaatgaa aaacttcata ttgcagaaga aaaaggcgat
    10441 ttggctgaat tgttagctaa gaatcttggt attgataaag ttcatttgat tcgttgcggt
    10501 ggcggaaatg ctgttgcagc tgctcgtgaa caatggaacg acggttcaaa cacccttaca
    10561 atagcaccag gtgtagtagt ggtatatgac cgtaacgttg ttacaaataa gattcttgaa
    10621 gaatacggac ttcgtttgat taagattcgc ggaagtgaat tggttcgcgg tcgtggtgga
    10681 cctcgttgta tgtcaatgcc atttgaacgt gaagaacttt aagattagaa agaggaaata
    10741 atagaatgac aaactcagtg ttccaaggac gcagcttctt ggcagaaaaa gattttacac
    10801 gtgcagaatt agaatacctt attggtttag cagctcactt gaaagatttg aagaaacgca
    10861 atattgaaca tcactaccta gcaggtaaaa atattgctct cttgtttgaa aaaacttcaa
    10921 ctcgtactcg tgcagctttc acaacagcag cgattgatct tggcgctcat ccagaatatc
    10981 taggagctaa cgacattcag cttggcaaga aagaatctac tgaagataca gctaaagtat
    11041 tgggacgtat gtttgacggg attgaattcc gtggatttag ccaacgtatg gtagaagaac
    11101 tagcagaatt ttcaggcgtt cctgtatgga atggtttgac agacgaatgg catccaactc
    11161 aaatgttagc tgactatttg acagttcaag aaaactttgg gcgtttggaa ggcttgactc
    11221 ttgtctactg tggtgacgga cgcaataacg ttgcaaacag tctccttgta acaggtgcta
    11281 tcctcggtgt aaatgttcat atcttttctc caaaagaact cttcccagct gaagatattg
    11341 tgaaattggc agaaggatat gcgaaagaaa gtggcgcaca tgtcttaatt actgacaatg
    11401 cagatgaagc tgttaaaggt gcggatgtcc tttacacaga cgtttgggta tctatgggtg
    11461 aagaagacaa atttgcagaa cgtgttgcac tgttgaaacc ttatcaagta aatatggatt
    11521 tgattaagaa agctgacaat gaagatttga tcttcttgca ctgcttacca gctttccatg
    11581 atacaaatac tgtttatggt aaagatgttg ctgaaaaatt cggcgttgaa gaaatggaag
    11641 taactgacga agtattccgc agcaaatacg ctcgccaatt tgaccaagct gaaaaccgta
    11701 tgcatactat taaagcagtt atggcagcaa ctcttggtaa cctttatatt ccaaaagtat
    11761 aatttaaaac caaaaccgtt taccaacagc tatgagggct gcgactaata gctttagtcc
    11821 agtcctcatt tttgatagtt cttgtgttca ttttagtatg atgttaatag aggaaaaagg
    11881 cgatagaaca aacaactata attactgatg cagtcactct cagagaaatg tagtctgaga
    11941 caatgctatt ctaacctcat tcctacgttt gttgcagctg gattctatct tggactacta
    12001 aaatgaaatt gaa
//
