GCF_005163865.1 - 6 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

NZ_CP035434.1 (Streptococcus pyogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	terpene-precursor	671,045	691,917
Region 2	RiPP-like	935,573	951,174
Region 3	T3PKS	1,152,202	1,193,377
Region 4	azole-containing-RiPP	1,290,146	1,313,930	High	streptolysin S	ribosomal:RiPP:LAP
Region 5	RiPP-like	1,466,507	1,478,397
Region 6	lanthipeptide-class-ii	1,604,662	1,627,496	High	salivaricin A	ribosomal:RiPP:Lanthipeptide

NZ_CP035434.1 - Region 1 - terpene-precursor

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Location: 671,045 - 691,917 nt. (total: 20,873 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthetase

ETT65_RS03675: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002698.2	0.40	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000487.5	0.36	ribosomal	carnocyclin	Carnobacterium maltaromaticum
BGC0000542.3	0.33	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.33	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000502.5	0.33	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001859.4	0.32	other (phosphonate)	fosfomycin	Pseudomonas syringae
BGC0000617.5	0.32	ribosomal	coagulin	Bacillus coagulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002698.2	0.40	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000487.5	0.36	ribosomal	carnocyclin	Carnobacterium maltaromaticum
BGC0000542.3	0.33	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.33	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000502.5	0.33	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001859.4	0.32	other (phosphonate)	fosfomycin	Pseudomonas syringae
BGC0000617.5	0.32	ribosomal	coagulin	Bacillus coagulans

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

ETT65_RS03675: PT_FPPS_like

Domain 1 (25 - 272): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.93 out of 26.33

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.16 out of 20.97

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.00 out of 26.85

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.26 out of 27.78

Confidence: medium

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 26.23 out of 28.14

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.55 out of 23.79

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.54 out of 28.19

Confidence: medium

Additional weak hits (4):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 25.95 out of 39.62

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.76 out of 27.55

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.56 out of 32.74

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.81 out of 30.25

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
IolR	Regulator of the inositol catabolism
PerR	Peroxide regulator
RutR	Regulator of the pyrimidine and purine metabolism
ZuR variant 1	Zinc-responsive repressor

NZ_CP035434.1 - Region 2 - RiPP-like

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Location: 935,573 - 951,174 nt. (total: 15,602 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002577.2	0.51	ribosomal	lactococcin G	Lactococcus lactis
BGC0000617.5	0.51	ribosomal	coagulin	Bacillus coagulans
BGC0002579.2	0.50	ribosomal	carnobacteriocin XY	Carnobacterium maltaromaticum
BGC0000558.5	0.49	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000624.3	0.48	ribosomal	salivaricin CRL1328 α peptide, salivaricin CRL1328 β peptide	Lactobacillus salivarius
BGC0000619.3	0.47	ribosomal	gassericin T	Lactobacillus gasseri
BGC0001388.3	0.47	ribosomal	gassericin E	Lactobacillus gasseri
BGC0001602.3	0.47	ribosomal	gassericin-T	Lactobacillus gasseri
BGC0002005.2	0.46	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0001862.4	0.44	ribosomal	pallidocin	Aeribacillus pallidus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002577.2	0.51	ribosomal	lactococcin G	Lactococcus lactis
BGC0000617.5	0.51	ribosomal	coagulin	Bacillus coagulans
BGC0002579.2	0.50	ribosomal	carnobacteriocin XY	Carnobacterium maltaromaticum
BGC0000558.5	0.49	ribosomal	sublancin 168	Bacillus subtilis subsp. subtilis str. 168
BGC0000624.3	0.48	ribosomal	salivaricin CRL1328 α peptide, salivaricin CRL1328 β peptide	Lactobacillus salivarius
BGC0000619.3	0.47	ribosomal	gassericin T	Lactobacillus gasseri
BGC0001388.3	0.47	ribosomal	gassericin E	Lactobacillus gasseri
BGC0001602.3	0.47	ribosomal	gassericin-T	Lactobacillus gasseri
BGC0002005.2	0.46	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0001862.4	0.44	ribosomal	pallidocin	Aeribacillus pallidus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 16.31 out of 18.16

Confidence: strong

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 22.31 out of 25.88

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.42 out of 24.78

Confidence: medium

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
LexA variant 2	Repressor of DNA damage response
PsrA	Oleic acid responsive regulator

NZ_CP035434.1 - Region 3 - T3PKS

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Location: 1,152,202 - 1,193,377 nt. (total: 41,176 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: reductase

ETT65_RS06070: hydroxymethylglutaryl-CoA reductase

smcogs: SMCOG1187: hydroxymethylglutaryl-CoA reductase

Hydrolases: amidase deformylase esterase

ETT65_RS05975: amidase

smcogs: SMCOG1105: amidase

ETT65_RS06110: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

smcogs: SMCOG1283: 2`,3`-cyclic-nucleotide 2`-phosphodiesterase

ETT65_RS06115: peptide deformylase

smcogs: SMCOG1275: peptide deformylase

Ligases: synthase

ETT65_RS06065: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002561.3	0.44	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000934.4	0.41	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001080.5	0.41	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000286.3	0.40	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0002284.3	0.39	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002362.3	0.38	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0001863.4	0.38	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0001521.3	0.38	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000679.5	0.38	terpene	diazepinomicin	Micromonospora sp. M42
BGC0000515.4	0.37	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002561.3	0.44	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0000934.4	0.41	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001080.5	0.41	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000286.3	0.40	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0002284.3	0.39	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0002362.3	0.38	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0001863.4	0.38	ribosomal, terpene	bacillicn CER074	Bacillus mycoides
BGC0001521.3	0.38	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000679.5	0.38	terpene	diazepinomicin	Micromonospora sp. M42
BGC0000515.4	0.37	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.77 out of 27.78

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.20 out of 24.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.60 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.30 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.93 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.33 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.36 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.42 out of 26.06

Confidence: medium

Additional weak hits (1):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 22.77 out of 28.14

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
LexA variant 2	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes

NZ_CP035434.1 - Region 4 - azole-containing-RiPP

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Location: 1,290,146 - 1,313,930 nt. (total: 23,785 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000566.5	0.97	ribosomal	streptolysin S	Streptococcus pyogenes M1 GAS
BGC0000564.5	0.69	ribosomal	clostridiolysin S	Clostridium botulinum A str. ATCC 3502
BGC0001170.5	0.64	ribosomal	clostridiolysin S	Clostridium sporogenes ATCC 15579
BGC0001171.5	0.50	ribosomal	listeriolysin S	Listeria monocytogenes serotype 4b str. F2365
BGC0000569.5	0.49	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001173.5	0.48	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0002700.2	0.41	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000605.5	0.40	ribosomal	GE37468	Streptomyces sp. ATCC 55365
BGC0001887.3	0.39	ribosomal	huazacin	Bacillus thuringiensis serovar huazhongensis BGSC 4BD1
BGC0002111.3	0.39	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000566.5	0.97	ribosomal	streptolysin S	Streptococcus pyogenes M1 GAS
BGC0000564.5	0.69	ribosomal	clostridiolysin S	Clostridium botulinum A str. ATCC 3502
BGC0001170.5	0.64	ribosomal	clostridiolysin S	Clostridium sporogenes ATCC 15579
BGC0001171.5	0.50	ribosomal	listeriolysin S	Listeria monocytogenes serotype 4b str. F2365
BGC0000569.5	0.49	ribosomal	plantazolicin	Bacillus velezensis FZB42
BGC0001173.5	0.48	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0002700.2	0.41	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000605.5	0.40	ribosomal	GE37468	Streptomyces sp. ATCC 55365
BGC0001887.3	0.39	ribosomal	huazacin	Bacillus thuringiensis serovar huazhongensis BGSC 4BD1
BGC0002111.3	0.39	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 18.98 out of 20.97

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.45 out of 26.06

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.06 out of 18.46

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (3):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.72 out of 30.22

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 22.53 out of 33.73

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.38 out of 27.18

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

RREFinder

TFBS Finder

Azole RiPPs

RRE predictions

: RRE type: Cytolysin_C_RRE; Location: 0 - 90; Score: 133.3

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CcpA	Catabolite control protein A
MogR	Repressor of flagellar motility genes
RpoN	Regulator of nitrogen assimilation and virulence

Core peptide predictions

No core peptides found.

NZ_CP035434.1 - Region 5 - RiPP-like

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Location: 1,466,507 - 1,478,397 nt. (total: 11,891 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	RiPP-like	Similarity score	Type	Compound(s)	Organism
BGC0000526.5		0.22	ribosomal	macedocin	Streptococcus macedonicus

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0000526.5		0.22	ribosomal	macedocin	Streptococcus macedonicus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.12 out of 23.79

Confidence: medium

Additional weak hits (2):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.57 out of 26.85

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 18.45 out of 30.12

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_CP035434.1 - Region 6 - lanthipeptide-class-ii

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Location: 1,604,662 - 1,627,496 nt. (total: 22,835 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: kinase

ETT65_RS08265: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Lyases: cyclase

ETT65_RS08235: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000548.4	0.77	ribosomal	salivaricin A	Streptococcus salivarius
BGC0001787.3	0.56	ribosomal	suicin 3908	Streptococcus suis
BGC0002698.2	0.51	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002005.2	0.50	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0001210.5	0.50	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000516.4	0.50	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000500.5	0.47	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000498.5	0.47	ribosomal	bovicin HJ50	Streptococcus equinus
BGC0000561.5	0.47	ribosomal	thermophilin 1277	Streptococcus thermophilus
BGC0001391.3	0.45	ribosomal	thusin	Bacillus thuringiensis serovar rongseni

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000548.4	0.77	ribosomal	salivaricin A	Streptococcus salivarius
BGC0001787.3	0.56	ribosomal	suicin 3908	Streptococcus suis
BGC0002698.2	0.51	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0002005.2	0.50	ribosomal	RaxX	Xanthomonas oryzae pv. oryzae
BGC0001210.5	0.50	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000516.4	0.50	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000500.5	0.47	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0000498.5	0.47	ribosomal	bovicin HJ50	Streptococcus equinus
BGC0000561.5	0.47	ribosomal	thermophilin 1277	Streptococcus thermophilus
BGC0001391.3	0.45	ribosomal	thusin	Bacillus thuringiensis serovar rongseni

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ETT65_RS08235

ETT65_RS08240

Leader:

MSFMKNSKDILTNAIEEVSEKELMEVAGG

Core:

KKGSGWFATITDDCPNSVFVCC

Core with dehydrations:

KKGDhaGWFADhbIDhbDDCysPNDhaVFVCysCys

antiSMASH-DB 4.0 matches for ETT65_RS08240

100.0%

NC_004070.1and 26 others:

NC_008024.1 Lanthipeptides: MGAS10750_RS08460_lanthipeptideNZ_CAAHHZ010000002.1 Lanthipeptides: E9518_RS02835_lanthipeptideNZ_CAAHIH010000002.1 Lanthipeptides: E9602_RS03585_lanthipeptideNZ_CAAHIO010000002.1 Lanthipeptides: E9584_RS04360_lanthipeptideNZ_CAAHJE010000002.1 Lanthipeptides: E9605_RS03435_lanthipeptideNZ_CAAHJS010000002.1 Lanthipeptides: E9628_RS02300_lanthipeptideNZ_CAAHKC010000012.1 Lanthipeptides: E9636_RS08085_lanthipeptideNZ_CAAHMR010000003.1 Lanthipeptides: E9727_RS05510_lanthipeptideNZ_CAAHQE010000003.1 Lanthipeptides: E9931_RS04945_lanthipeptideNZ_CAAHQG010000004.1 Lanthipeptides: E9903_RS05690_lanthipeptideNZ_CAAIFD010000008.1 Lanthipeptides: FAG63_RS07845_lanthipeptideNZ_CAAJDK010000010.1 Lanthipeptides: FAO79_RS08000_lanthipeptideNZ_CAAJFI010000003.1 Lanthipeptides: FAP60_RS03740_lanthipeptideNZ_CP008926.1 Lanthipeptides: DP15_RS03855_lanthipeptideNZ_CP035434.1 Lanthipeptides: ETT65_RS08240_lanthipeptideNZ_CP035435.1 Lanthipeptides: ETT64_RS07905_lanthipeptideNZ_CP035447.1 Lanthipeptides: ETT52_RS07920_lanthipeptideNZ_CP035449.1 Lanthipeptides: ETT50_RS08255_lanthipeptideNZ_CP035453.1 Lanthipeptides: ETT46_RS08220_lanthipeptideNZ_CP060646.1 Lanthipeptides: H7789_RS07920_lanthipeptideNZ_CP065054.1 Lanthipeptides: I2432_RS09105_lanthipeptideNZ_LRGW01000003.1 Lanthipeptides: AWU01_RS03260_lanthipeptideNZ_LS483298.1 Lanthipeptides: DQM39_RS08650_lanthipeptideNZ_LS483407.1 Lanthipeptides: DQM86_RS08100_lanthipeptideNZ_LS483425.1 Lanthipeptides: DQN36_RS07865_lanthipeptideNZ_LS483430.1 Lanthipeptides: DQN28_RS07600_lanthipeptide

Lanthipeptides: SPYM3_RS08605_lanthipeptide

1

KKGSGWFATITDDCPNSVFVCC

22

KKGSGWFATITDDCPNSVFVCC

1

KKGSGWFATITDDCPNSVFVCC

22

95.5%

NZ_CAAIJM010000014.1:

Lanthipeptides: FAI23_RS09465_lanthipeptide

1

KKGSGWFATITDDCPNSVFVCC

22

KK SGWFATITDDCPNSVFVCC

1

KKVSGWFATITDDCPNSVFVCC

22

90.9%

NZ_CAJHJN010000010.1and 4 others:

NZ_CP066093.1 Lanthipeptides: I6I02_RS06995_lanthipeptideNZ_JACLQK010000029.1 Lanthipeptides: H7U04_RS10750_lanthipeptideNZ_JADPEB010000003.1 Lanthipeptides: I4U26_RS02855_lanthipeptideNZ_KV832031.1 Lanthipeptides: HMPREF2637_RS05170_lanthipeptide

Lanthipeptides: JMZ68_RS08975_lanthipeptide

1

KKGSGWFATITDDCPNSVFVCC

22

K+G+GWFATITDDCPNSVFVCC

1

KRGTGWFATITDDCPNSVFVCC

22

with 66 more sequence matches

Only the first 10 are displayed here.

86.4%

NZ_JAQDVH010000019.1and 3 others:

NZ_JAQDVH010000019.1 Lanthipeptides: PGU04_RS09335_lanthipeptideNZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06620_lanthipeptideNZ_JAQMJT010000006.1 Lanthipeptides: PNU26_RS06620_lanthipeptide

Lanthipeptides: PGU04_RS09335_lanthipeptide

1

KKGSGWFATITDDCPNSVFVCC

22

KKG GW ATITDDCPNS+FVCC

1

KKGPGWIATITDDCPNSIFVCC

22

86.4%

NZ_PKIA01000004.1:

Lanthipeptides: CYK22_RS03780_lanthipeptide

1

KKGSGWFATITDDCPNSVFVCC

22

K+G GW ATITDDCPNSVFVCC

1

KRGPGWIATITDDCPNSVFVCC

22

81.8%

NZ_CP018188.1and 1 other:

NZ_VDNV01000006.1 Lanthipeptides: FSV44_RS08680_lanthipeptide

Lanthipeptides: BSR19_RS10820_lanthipeptide

1

KKGSGWFATITDDCPNSVFVCC

22

K+G GW ATITDDCPNS+FVCC

1

KRGPGWIATITDDCPNSIFVCC

22

77.3%

NZ_CP048273.1and 3 others:

NZ_CP048273.1 Lanthipeptides: M654_RS18555_lanthipeptideNZ_LT745776.1 Lanthipeptides: B2C81_RS11280_lanthipeptideNZ_LT745776.1 Lanthipeptides: B2C81_RS11275_lanthipeptide

Lanthipeptides: M654_RS18560_lanthipeptide

2

KGSGWFATITDDCPNSVFVCC

22

G GW TITDDCPNSVFVCC

1

AGCGWACTITDDCPNSVFVCC

21

77.3%

NZ_CP048890.1and 3 others:

NZ_CP071478.1 Lanthipeptides: J0P05_RS32905_lanthipeptideNZ_LUCO01000008.1 Lanthipeptides: BMBG_RS24280_lanthipeptideNZ_NVLN01000014.1 Lanthipeptides: CON11_RS24965_lanthipeptide

Lanthipeptides: BMG_RS30865_lanthipeptide

2

KGSGWFATITDDCPNSVFVCC

22

G GW TITDDCPNSVFVCC

1

AGCGWSCTITDDCPNSVFVCC

21

77.3%

NZ_NIHT01000008.1and 1 other:

NZ_NIHT01000008.1 Lanthipeptides: CDL23_RS06740_lanthipeptide

Lanthipeptides: CDL23_RS06745_lanthipeptide

2

KGSGWFATITDDCPNSVFVCC

22

GSGW TITDDCPNS+FVCC

2

NGSGWLPTITDDCPNSIFVCC

22

72.7%

NZ_CP036073.1and 11 others:

NZ_CP036073.1 Lanthipeptides: EXW45_RS04930_lanthipeptideNZ_CP036073.1 Lanthipeptides: EXW45_RS04925_lanthipeptideNZ_CP036073.1 Lanthipeptides: EXW45_RS04920_lanthipeptideNZ_CP036073.1 Lanthipeptides: EXW45_RS04915_lanthipeptideNZ_CP036073.1 Lanthipeptides: EXW45_RS04910_lanthipeptideNZ_NUEG01000031.1 Lanthipeptides: CN905_RS18915_lanthipeptideNZ_NUEG01000031.1 Lanthipeptides: CN905_RS18910_lanthipeptideNZ_PVRT01000001.1 Lanthipeptides: C6359_RS05275_lanthipeptideNZ_PVRT01000001.1 Lanthipeptides: C6359_RS05270_lanthipeptideNZ_PVRT01000001.1 Lanthipeptides: C6359_RS05265_lanthipeptideNZ_PVRT01000001.1 Lanthipeptides: C6359_RS05260_lanthipeptide

Lanthipeptides: EXW45_RS04935_lanthipeptide

2

KGSGWFATITDDCPNSVFVCC

22

G GW T+TDDCPNSVFVCC

1

AGCGWLCTVTDDCPNSVFVCC

21

72.7%

NZ_JANHJQ010000013.1:

Lanthipeptides: NON16_RS14160_lanthipeptide

2

KGSGWFATITDDCPNSVFVCC

22

G GW T+TDDCPNSVFVCC

1

DGCGWSCTVTDDCPNSVFVCC

21

68.2%

NZ_MJEH01000022.1and 1 other:

NZ_MJEH01000022.1 Lanthipeptides: BFG57_RS18360_lanthipeptide

Lanthipeptides: BFG57_RS18365_lanthipeptide

3

GSGWFATITDDCPNSVFVCC

22

G GW +TITDDCPNS+ VCC

1

GDGWISTITDDCPNSIIVCC

20

68.2%

NZ_MOWR01000001.1:

Lanthipeptides: BMI76_RS01705_lanthipeptide

2

KGSGWFATITDDCPNSVFVCC

22

+ GW TITD+CPNSVFVCC

1

RWYGWSFTITDECPNSVFVCC

21

MIBiG 4.0 matches for ETT65_RS08240

17.4%

BGC0002111.3 (EVG22_32245):

Lanthipeptide: andalusicin A or andalusicin B

5

GWFATITDDC

14

W +TI+++C

14

AWASTISNNC

23

allorf_1624812_1624904

Leader:

MKKINIFCLFFIKINKKMLTFMFGG

Core:

CMIRS

Core with dehydrations:

CysMIRDha

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.03 out of 26.06

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.03 out of 26.06

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 24.03 out of 26.06

Confidence: strong

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.28 out of 29.38

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.23 out of 26.06

Confidence: medium

Additional weak hits (3):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 26.44 out of 39.62

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.35 out of 19.52

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.24 out of 32.74

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

ETT65_RS08240 - Class II

Cleavage pHMM score: 4.40

RODEO score: 0

Monoisotopic mass: 2305.1 Da

Molecular weight: 2306.6 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2324.6 Da N = 1

allorf_1624812_1624904 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 590.3 Da

Molecular weight: 590.8 Da

Number of bridges: 1

TFBS definitions

Regulator	Description
FuR variant 1	Ferric Uptake Regulator
MogR	Repressor of flagellar motility genes