LOCUS       NZ_CP035434            11891 bp    DNA     circular CON 24-JUL-2024
DEFINITION  Streptococcus pyogenes strain emm75.1 chromosome, complete genome.
ACCESSION   NZ_CP035434
VERSION     NZ_CP035434.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1887700)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Atlas of group A streptococcal vaccine candidates compiled using
            large scale comparative genomics
  JOURNAL   Nat. Genet. (2019) In press
REFERENCE   2  (bases 1 to 1887700)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005163865.1-RS_2024_07_24
            Annotation Date                   :: 07/24/2024 01:48:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,868
            CDSs (total)                      :: 1,779
            Genes (coding)                    :: 1,714
            CDSs (with protein)               :: 1,714
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 36 of 65
            Pseudo Genes (incomplete)         :: 31 of 65
            Pseudo Genes (internal stop)      :: 25 of 65
            Pseudo Genes (multiple problems)  :: 24 of 65
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:28:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1466506
            Orig. end                         :: 1478397
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035434.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11891
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11891
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11891
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6891](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6891)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            195..437
                     /locus_tag="ETT65_RS09850"
                     /old_locus_tag="ETT65_07450"
     CDS             195..437
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285423.1"
                     /locus_tag="ETT65_RS09850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07450"
                     /product="hypothetical protein"
                     /protein_id="WP_032460069.1"
                     /transl_table=11
                     /translation="MKQQEHTVMMCLNKTLTIKLSAPLTSNDLVPLSTNEVIIMEPLLH
                     LLPKVVPVLIHLVDKANVSWLTVILQLPILLKTIW"
     gene            959..1228
                     /gene="stcA"
                     /locus_tag="ETT65_RS07465"
                     /old_locus_tag="ETT65_07455"
     CDS             959..1228
                     /codon_start=1
                     /gene="stcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /locus_tag="ETT65_RS07465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07455"
                     /product="quorum-sensing system protein StcA"
                     /protein_id="WP_023605037.1"
                     /transl_table=11
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHSK
                     QNPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQS"
     gene            complement(1576..1974)
                     /locus_tag="ETT65_RS07470"
                     /old_locus_tag="ETT65_07460"
     CDS             complement(1576..1974)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /locus_tag="ETT65_RS07470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07460"
                     /product="hypothetical protein"
                     /protein_id="WP_011054254.1"
                     /transl_table=11
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLED
                     DAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTGIT
                     YGDDTLADLTHHLLHSDGVIPSLNDKSA"
     gene            complement(2067..3136)
                     /locus_tag="ETT65_RS07475"
                     /old_locus_tag="ETT65_07465"
                     /pseudo=""
     CDS             complement(2067..3136)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /locus_tag="ETT65_RS07475"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07465"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MMMLRDVKVKTSSLLVGCAATLLVSLSSTVAAESVHSSDRRFGVG
                     YKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQIVPG
                     MGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTVFLV
                     QPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTRGSGAFPDNIKVYIN
                     PKGGRPRLLMGMALRILFLQQFYGKKAP"
     gene            complement(3387..3788)
                     /locus_tag="ETT65_RS07480"
                     /old_locus_tag="ETT65_07470"
     CDS             complement(3387..3788)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /locus_tag="ETT65_RS07480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07470"
                     /product="hypothetical protein"
                     /protein_id="WP_023605036.1"
                     /transl_table=11
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDLF
                     VFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEVSIYFYLHL
                     IVVCYTIGMILSLGRLISPNHSIFNKLKK"
     gene            complement(3982..4250)
                     /locus_tag="ETT65_RS07485"
                     /old_locus_tag="ETT65_07475"
                     /pseudo=""
     CDS             complement(3982..4250)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528350.1"
                     /locus_tag="ETT65_RS07485"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07475"
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKILDIIFNLSDRKMIFLENVEKLGGYIVPFTLIILSVAYNFRHP
                     YPIWAAVLGGISLIVIFILFIYICVIDTWYGKKYQKLRLILLSY"
     gene            complement(4300..4494)
                     /locus_tag="ETT65_RS07490"
                     /old_locus_tag="ETT65_07480"
     CDS             complement(4300..4494)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992021.1"
                     /locus_tag="ETT65_RS07490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07480"
                     /product="hypothetical protein"
                     /protein_id="WP_002990768.1"
                     /transl_table=11
                     /translation="MEVLKNGLAIINGGGKDGANIFLTGMAGAAQGVTVCAQTGVFIPW
                     QGYILCGAAGAATNIIWPH"
     gene            complement(4788..4988)
                     /locus_tag="ETT65_RS07500"
                     /old_locus_tag="ETT65_07490"
     CDS             complement(4788..4988)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /locus_tag="ETT65_RS07500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07490"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_003060803.1"
                     /transl_table=11
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHPV
                     LTGGCVVGFTVGGAYLGYTAN"
     gene            complement(5001..5228)
                     /locus_tag="ETT65_RS07505"
                     /old_locus_tag="ETT65_07495"
     CDS             complement(5001..5228)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016754.1"
                     /locus_tag="ETT65_RS07505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07495"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_002992018.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 7.6e-17,
                     bitscore: 58.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAIG
                     FPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            5543..6229
                     /locus_tag="ETT65_RS07510"
                     /old_locus_tag="ETT65_07500"
                     /pseudo=""
     CDS             5543..6229
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /locus_tag="ETT65_RS07510"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07500"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTQFTTELLNFLAQKQDIDEFFRSSLEIAMNDLLQVERSAFLGYE
                     PYEKEGYNTGNSRNGTYSRQFETKYGLVNLIIPRDRNGEFSPVLLPSYARREDHLEEIV
                     IKLYQTGVTTREISDIIERMYGHHLFLSGSLVTKRL"
     gene            complement(6449..6631)
                     /locus_tag="ETT65_RS07515"
                     /old_locus_tag="ETT65_07505"
     CDS             complement(6449..6631)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /locus_tag="ETT65_RS07515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07505"
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="WP_002987564.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3e-07, bitscore:
                     27.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNFLELQFEELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITGG
                     AAAFVCVASGIAAYQ"
     gene            complement(6646..6891)
                     /locus_tag="ETT65_RS07520"
                     /old_locus_tag="ETT65_07510"
     CDS             complement(6646..6891)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /locus_tag="ETT65_RS07520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07510"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_002994504.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2e-15, bitscore:
                     53.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGGVGAG
                     MATLGVGGVAGAFAGAHVGAIAGGLTCVGGMLFNGK"
     misc_feature    complement(6848..6862)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.12"
                     /tool="antismash"
     gene            7289..7543
                     /locus_tag="ETT65_RS09735"
                     /old_locus_tag="ETT65_07515"
     CDS             7289..7543
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990779.1"
                     /locus_tag="ETT65_RS09735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07515"
                     /product="hypothetical protein"
                     /protein_id="WP_011106868.1"
                     /transl_table=11
                     /translation="MSGLFYHKSYFFKSEQVYDLLNEIRMRYPHAHIILIGNHINYEEI
                     FKNHYRVFGVIDTTSHKSLRSIRDQIQLYLDELYNSNNG"
     gene            complement(7850..8326)
                     /locus_tag="ETT65_RS07530"
                     /old_locus_tag="ETT65_07520"
     CDS             complement(7850..8326)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /locus_tag="ETT65_RS07530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07520"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_136096066.1"
                     /transl_table=11
                     /translation="MPQDYISYIRSKVEHDKIILNFAGGILTNDDGKVLMQLRGDKKTW
                     AIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFIYE
                     LTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     gene            complement(8346..9242)
                     /gene="era"
                     /locus_tag="ETT65_RS07535"
                     /old_locus_tag="ETT65_07525"
     CDS             complement(8346..9242)
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /gene="era"
                     /gene_functions="other (smcogs) SMCOG1227: ribosome
                     biogenesis GTP-binding protein YsxC"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /locus_tag="ETT65_RS07535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07525"
                     /product="GTPase Era"
                     /protein_id="WP_002985743.1"
                     /transl_table=11
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIMG
                     IYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDDMI
                     IERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTLIKL
                     LTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKRDEET
                     DKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVKVKKNW
                     RDKKLDLADFGYNEKEY"
     misc_feature    9295..9310
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.57"
                     /tool="antismash"
     gene            complement(9362..9769)
                     /locus_tag="ETT65_RS07540"
                     /old_locus_tag="ETT65_07530"
     CDS             complement(9362..9769)
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /locus_tag="ETT65_RS07540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07530"
                     /product="diacylglycerol kinase family protein"
                     /protein_id="WP_002985746.1"
                     /transl_table=11
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLLS
                     ACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNAKD
                     MAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            complement(9750..10247)
                     /gene="ybeY"
                     /locus_tag="ETT65_RS07545"
                     /old_locus_tag="ETT65_07535"
     CDS             complement(9750..10247)
                     /GO_function="GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ybeY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /locus_tag="ETT65_RS07545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07535"
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="WP_002985748.1"
                     /transl_table=11
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVTN
                     ERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDLAADPSLAEMMAEFDAYIGELFI
                     SIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAYGLT
                     RQ"
     misc_feature    complement(10261..10275)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 18.45"
                     /tool="antismash"
     gene            complement(10406..10981)
                     /locus_tag="ETT65_RS07550"
                     /old_locus_tag="ETT65_07540"
     CDS             complement(10406..10981)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /locus_tag="ETT65_RS07550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_07540"
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="WP_009880369.1"
                     /transl_table=11
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARIIIVGQAPGIVA
                     QETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFAAK
                     WHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPSPRN
                     QLWLAKNPWFEQDLLPILQKRVEAILTK"
ORIGIN
        1 ctttttacat tgatcatgga tatcctgatt atcgttggtg gataaccttt cgcttaaaaa
       61 caatccactt ttgggaaacc cttatattta gtttgtaaca ggaaaactaa taaatattaa
      121 aaagagtgtt agcgacctag cggcagcttt actcttaagt gcgtttgctg ccattgacag
      181 caaggtcaaa gctgatgaaa cagcaggagc ataccgttat gatgtgtctg aacaaaacac
      241 ttactatcaa actatcagct ccattaacca gcaacgacct ggtgccactc tcgacgaatg
      301 aggtgattat tatggaaccc ttacttcatt tacttcccaa agtcgtacca gtacttatac
      361 acttggtaga caaagccaac gtatcatggt taacagtgat attacagtta cctatactct
      421 taaagacaat ttggtagccc ttcataagga atacaaaacc tttgtcaaaa aaatattaca
      481 gataaaccga tcaccagtga ctacgacaag gctaaggtta tttataacca tttaatcaac
      541 gaatacactt acgccacaaa ataacttggc accaatcaca aaaccgtaag cagcatcaac
      601 aatcatactc ctaaagctct ttatcgtggc aagtgtggtg cttgtcaagc ttatgccgtc
      661 atgtttaaag atatggcaga agctgcaggc ttatcaactt ggtatgtgat aaaaaagaat
      721 tgaatgagca ccatgcttga aacatcgtta ccattcatgg tgttaattcc tacgttgatg
      781 ctacctggaa tgattcagca tacaccacaa ggtatttcct tgctggaaaa gacgtgagga
      841 atactgagta ccgtttagat agccagtaca accacttggc taatgctatt ttaaatacca
      901 gctaccaaaa aggctaaact aacaacaact acatttttca actagaaagg cctaaacaat
      961 gaccaaaaaa cagttttatc ttatcttagc tattagcata ctgtgtttta ttcttgccgt
     1021 cagccctaaa ccaccaattc ctaaacacac tattgaaagg aaaaggactg cgatgaccat
     1081 aggccactca aaacaaaacc ctcacaaaaa agaagccctt aaacacaaaa aaaacaaaaa
     1141 taaggctctt tttcttccaa gtggggacag cgctatcaaa tctgttatta gcatgacagt
     1201 tagactcctg ttagatgacc aatcttagct ctcctaaaat cagtggacac taccatttcg
     1261 gtagtgttct tttttaagct aatctcatca ccacgctttc ttcaagctaa aaaactgatc
     1321 atgaaagagc aaccccatca ccctaacagc ccactttgcc cgcactttct taaaaagtta
     1381 ggagaaaacg atataccaat tattttctga tcccagctcc ctatcattag caagatgtgt
     1441 agcacggacc tacatacagg taagctagag tcattctcgc tatttcggac aaaaagaggc
     1501 cggtagttcc ctgacctctt ctttatttat cttgtaacac tcctcttctt agcgagactt
     1561 actagcacat aattcttaag cactcttatc atttagacta ggaataacac catctgaatg
     1621 aagcaaatga tgcgtcagat ctgccagagt atcatctcca taagtaatcc ctgtttggtg
     1681 ctgtttctga tgaaaatcag atgactcctt aactttagca gtcacctgct ggtcacttgg
     1741 atgaaaatta atttctctct tggcttcagc cgcaactggc tcgaggaaca acttattgac
     1801 attacggtct tcctccaaat gattaaggat agcagcatca tcttctaacg aaagaacctt
     1861 cttgggtaac aaaaaggggt tatttagcct tggctcagta acgacttcct tggctactcg
     1921 tgatagttta ttattgtctt cctcaggtat caaacgatta ggcaatcgat gcaattcttc
     1981 cggctgtgag gcgctcacag ctacttcaga agaaacctcc aagccctttc ctgtttgcct
     2041 gtctaaagca accggcaaat tattaactaa ctttttaacc tgtcctcagc gctcatttca
     2101 acactaggaa gagctgtcgc tggttctagc cagctaccaa cttctgctgg tcttacctta
     2161 acatcgctgg caggcttctc cttctcatct tcattaggtc taaaaggctc aaaccgccct
     2221 gttgattttt catctatgac ctttatattg tcttttacta catgagacag tggttcgaca
     2281 tttaccatat cttcatgctc gttaaggctc tggtctcgtt tgaatttacc aggaatacca
     2341 ggagtcggtg ttccgacaga ttgatcggct tcagtaggct cttttacgga gccttttttc
     2401 cataaaactg ctggaggaat aatatccgta gagccatccc cataagtaat cgtggcctgc
     2461 ccccctttgg attaatatat actttaatat tgtctgggaa agccccacta cctcttgtgg
     2521 ttaaaagggc acccgcattt ttaatgagag ttgtcattcg ttcaatacca gcaacgccaa
     2581 tatggttcaa gtcatctact ggtaactgat caacagattt catggtgtat ttccaagctt
     2641 gcggttgaac aagaaaaaca gttcctttat acattgtgtt atgcatatca tcaatgactt
     2701 tgaaatacct tgtatatttg gtttgtacat tagggttagc aacgggaaca gtgctttcag
     2761 gaactcctga catcgtaaaa atccctctag cagaagtttg aaagccctca taaacatgac
     2821 ccataccagg aacaatctga gcggtcaaag acttaccaga accatccaaa gcaatgcgaa
     2881 tatctttcat cagatacatc tgattggtgt aggtaaacct aaccgtgtta gcctcacctc
     2941 gatacaatac cacttgggag ggctgatccg aagaaaactg agaggttgtt tcttccttat
     3001 agccaactcc aaaacgtcta tcagacgaat ggactgactc tgccgcaact gtactactca
     3061 aactgactag taaagtagcc gcacaaccaa ctaataaact gctcgtttta actttaacat
     3121 ctcttaacat catcatgttc ctttcattat tttttattta ggaattcgtc atactatatt
     3181 ttctcatatt agtctaacaa aaaactcgta attctatata aatcatataa aatgacgagc
     3241 tttggcaaag atgagtatag caggatggaa aacagattgt ttctgaaact tcaaaggtat
     3301 cttcttgtca cgaactattc tttaattgaa tcggttacac taaactagtc ccattattta
     3361 aaaacaaact ttaaatatga ttcatttcac tttttaagtt tattaaatat agagtgatta
     3421 ggagaaataa gacgacctaa tgataaaatc attcctattg tatagcaaac aacaataagg
     3481 tgtaaataaa agtaaattga tacctctgat atactcgtca aacatcctat tattataata
     3541 aaagcaatta aggtaggaat aagaattgta cacttaataa ataaccaaaa ttgcttaaca
     3601 acttgatacc atgtgagcac ttctgtcgtt tgtctttgtt tgtagacaaa aacgaataaa
     3661 tcaaggataa aactccaaca gataagttgt atatttttca ttaatggtaa atcgtagaaa
     3721 ctacctatac taaatagaat cactgcagaa ataataacaa tacctaaaaa tcttaatata
     3781 ttacgcatat cttcacctcc tctactctat cggtagcaaa tataaccgaa caaattatta
     3841 ttcctgttat cagagacagt tataactttt ttaaccaatt ttcaatacgc attttgcttt
     3901 taactcttta gtctctaaag ttaaatatca taagtactga tattgtataa gtcatccatt
     3961 ttccccttaa atgtgattgc attatagctc agtaaaatta atcttaattt ctggtatttt
     4021 tttccatacc aagtatctat aacgcaaata tagataaaca aaataaagat cacaataagg
     4081 gatatacctc ctagtacagc agcccaaata ggataagggt gcctaaagtt gtaggctaca
     4141 ctcaagatga taagggtaaa agggacaatg taacctccca gcttttcaac attttctaga
     4201 aatatcatct ttctatcaga taggttaaaa ataatatcta aaattttcat accactcctc
     4261 tactacttat acatcagtga tagatagtca tttatatatt tagtgaggcc agataatatt
     4321 tgtagcagca ccagctgctc cacacaaaat ataaccttgc caagggataa atacgcctgt
     4381 ctgagcacaa actgtcactc cctgcgcagc tcctgccatc cctgtcaaaa agatatttgc
     4441 tccgtctttt ccgcctccat ttatgatagc cagaccattt tttagcactt ccatattttt
     4501 gaagtctttt tcattttaat ttcactcttt tgtattttaa aaagctaaga gtgatatcta
     4561 aattcattgg cataatcact actttctttt atcgataaaa acaaataatg acgctaccaa
     4621 agctacgata gataacatgt ttaccgattt ttccgaaagg gataattgat tgaaaattaa
     4681 aaacactaaa tatggtatag atttatcaac atttttttat gaatggcatc aacaatgatt
     4741 aaaaacatta tagacgcatg cataataata aaaatcgtat agatcattta atttgctgta
     4801 tatcccaaat atgctccacc aactgtaaat cctacaacac atccacctgt caatactgga
     4861 tgtggcagtt tacaaaattt caatccagaa gctaagcctc caagaccacc aattacagca
     4921 tctcctggag cgtttcctcc acttatacta gccatgtcat taattgttaa ttccttcatt
     4981 gttgtcatcc tttctttcct ctagaagccg cctgtagctc ctgttacacc agcccaaaga
     5041 gtaatagctg ttttagctcc aatataacca caggctacac cgcaaactgg aaaacctata
     5101 gcagctccag cactacctgc agcgataacc ccactgacat ttgcctgcca attattctta
     5161 ccaccctcaa ccttggcaag cttttctata gtcatttggt gaaatgtttc cagtttcttt
     5221 atctccatat cgaataactt cttttcataa ttcttaaatt tgtctcgttg caacctcgag
     5281 ctagtacaga atatcataaa gaacagtatc atggtttcaa aatcttaaaa ggctgttttt
     5341 catcatgaaa tgtcttttta tcctctctac acttacgctg tgacaatagt taattaagaa
     5401 gtttaaataa taccattcat gcttttataa atacctttta agatttttga tggtagtgta
     5461 aaatatcttg tataaacaca caaaaaggaa taaatcctgt atagtagagt tacgacactt
     5521 cactagaaag agatttattc ccatgactca gtttaccaca gaattactta acttcctagc
     5581 acaaaaacaa gatattgatg aattctttcg ctcctcttta gaaatagcta tgaatgacct
     5641 cttgcaagtt gaacgatcag cttttcttgg ttatgaacct tatgaaaaag aaggttacaa
     5701 cacaggtaat agccgtaatg ggacttattc tcgtcagttt gaaacaaagt atggcttagt
     5761 caatttaatc atcccaaggg atcgaaacgg cgagttttca ccagttttat taccatctta
     5821 tgctagacga gaggaccact tggaagagat agtgatcaaa ctctatcaga ctggcgttac
     5881 gacacgcgaa atcagtgaca ttattgagcg catgtatggt caccacttgt ttctgagtgg
     5941 aagcctcgtt acaaaaaggt tatgacatca cttgaaacaa cggaaaatct cctaactttc
     6001 tatcaatttc cacattacat ttggtctagt atttactcta caaacttgat tgaatcactc
     6061 aataaagaaa tcaagcgaca aagcaagaag gagggtggtt tttccaaatg aagaagcctt
     6121 agaacgatgt cttgtaagta ttttcgaaga ctataatatt aagttcggag ttcgtattca
     6181 taaaagattc ggagtatgtt ttgacacact tgatagctta tttgactaat attcaaaaac
     6241 tctactcttg tgtttacaca acattattga cagtctcaat taatctatat ccctttaata
     6301 ttattaattt gtaatagtac ttagtaagac ataaacaaat atcacaatta aaattacttt
     6361 acttgcattt tctcttacct ctttatcctt caaaaaaatt tgggaaagta ttagacaaat
     6421 taaaccgagt attataacta taatcatatc attgataagc tgcaattcca gatgctacac
     6481 atacaaatgc tgccgcacct ccagttattg gacctcctgc tgcagctata agcatcccac
     6541 ctaagcaccc tccgatagca gatccaatat ttccttttcc accagaaata tttactaatt
     6601 cttcaaactg taattctaaa aaattatcca tttttcacct caaaattatt ttccattaaa
     6661 taacatacca ccaacgcaag ttaatccacc cgcaatagca cctacatgtg cacctgcaaa
     6721 cgcacctgca actcctccaa cacctaaagt agccatacct gctccaacac cacctgcaat
     6781 agcacctgaa gtaataccac caattaaatg atttttacaa tctgtagcac tataacctcc
     6841 aataatatgg aacaactctt ctttttgtat ttcttctgca aattttatca taactttaaa
     6901 aacctttcta aaatgttata ttcatcttgt tgcaactttg acaaactaaa tatagcatat
     6961 tattataaaa atttttttct atgtcgtgaa tggttgtata aacatctcaa ataaactttt
     7021 taacatatta gagactgtca ataatgttgt gtaaacacaa gagtagagtt tttgaatatt
     7081 agtcaaataa gctatcaagt gtgtcaaaac atactccgaa tcctttatga atacgagctc
     7141 cgaacttaat attatagtct tcgaaaatac ttacaagaca tcgttctctt ttaggatata
     7201 tgatatctat ctatattaac tattacctca agagatactt tctaaaaact caaattatta
     7261 gtgggaaaaa cttagattat aaatacaaat gtcaggttta ttttatcata aatcatactt
     7321 ttttaaatct gagcaagtat atgaccttct aaacgaaata cgtatgcgtt atcctcatgc
     7381 tcatattatt ctaatcggaa atcatattaa ttacgaggaa atatttaaaa atcattatcg
     7441 agtttttggt gttattgaca caacaagtca taaatcatta aggtccattc gagaccaaat
     7501 tcaactttac ttagacgaac tttacaattc gaataatgga taggcacttc atcaattctt
     7561 ttaagaaatg attcttgtca cccccttcca agtctattgc cacagttagg ttcaacttgc
     7621 ctaagtcatg catggctagg tggcaattcg tcataactct aaaacaaatg tttgatgtct
     7681 gctagtatca attggaaaga aaatcctgtt aacatttaac aaaattagtc agacttaatc
     7741 agaaaaattg gaagcaaaaa aagagagcat aagctctcga aacatcgtct ttcaaaaatt
     7801 tttttaacaa gccaatctcc aaaaatactc caactgtcct agagagttct taatgcccca
     7861 tagcaaagct atcactaaaa tattcctcta acatcaggcg atgtttggct gagacactct
     7921 ctaactctgc tatctcctca tgagagaaaa actgtaactt gagcgtttcc tcattatgaa
     7981 agttatcaat agccatatca gaaacagccg ttagttcata gataaagaca atagtctgaa
     8041 cagcatcacc gttgggataa acttcctcaa aatgagtgta aacattcagt aagcgaacag
     8101 cttcaacctc aattccggtt tcctctaaga actcacgttt gcaagtctct aaagagctct
     8161 cacccagttc catagtgcca ccaggaatag cccaggtttt cttatcacca cgcaactgca
     8221 tcagcacctt gccgtcatca ttggtcaaaa tcccaccagc aaagttaaga ataatcttat
     8281 catgctcaac tttggagcgg atataagaaa tgtagtcttg tggcatatga tctcgcttta
     8341 ctatgttagt attctttctc attatagcca aaatcagcta aatcaagctt tttatcccgc
     8401 cagtttttct tgactttgac ccacgtttct aagtagacct tgtcacctaa catgagttca
     8461 atatcacggc gcgccatttt tccgattttt ttgagcatgg ctccttgttt tccaatgatg
     8521 atgccttttt ggctatcgcg ctccaccata atggttgcac ggatatgcac tttatcagtc
     8581 tcttcatctc gcttcatgga ttcgacaaca acagctacgg aatgtggaac ctcttgttgc
     8641 gtcaaatgca gtaccttctc acgaaccatt tctgacacaa ggaaacgttc tggatgatca
     8701 gtaatctgat cttctggaaa atattggaaa ccttcttcaa gattatcagt taataacttg
     8761 attagggttg gcacattatt accttctagg gcagaaattg ggaccacttc tttaaaatcc
     8821 atttggctac ggaagtcatc aatctgttcc aaaagttggt ctggatgaac cttatcaatt
     8881 ttgttgataa ctaaaataac aggaatctta gctgctttta gccgctcgat aatcatgtcg
     8941 tcacccttac cacgcttttc atcggcagga accatgaaaa gaaccgtctc aacttcacgt
     9001 aaagtactat aagcagattc aaccataaaa tcaccaaggg cggtctttgg tttatgaata
     9061 cctggcgtat caataaagac aatttgttct gtttctgtgg tgtaaatgcc cataatttta
     9121 ttacgagtgg tctgagcctt gtcactcatg atggcaattt tttgccccat gacgtgattt
     9181 aaaaaggtgg atttgccaac atttggacga ccaagaatgg ctacaaagcc tgatttaaac
     9241 atagataatt acgggcctta acggtagggc ttcagatcaa gcttgtcact aagcttaacc
     9301 gtcttgtcct ttccttattc tcctatgcca tcatcacata ggatagccca ttcctttctt
     9361 tttaatgaac gaaaatattc caaattttag gaataaaaat aataagtcct gtcaagacag
     9421 catagccaga aatcatcaaa acagcacctg ctgccatatc cttggcattt ttagctagca
     9481 tagaaaagtg atagtcacta gccaaatcaa ctacattttc aatagcagaa ttgacaattt
     9541 ctaaggtaat gaccaaaaaa atggctagaa gtaaaaataa ccattcgatg gctgaaatgc
     9601 taaaaaataa accagcaaca caagctaaac aggcagacaa aaggtgactt ctgagattgc
     9661 gttcttcctt aaaagctgtg aaaacccctg tcaaggcaaa ctccaggctt gaggtaatgg
     9721 ttctgttttt ccatttccgt ttggtggtat tattgtcgtg taaggccata agcagtcaaa
     9781 atctcttcct gtaaggtaaa catctctttt tcttcttcaa gggtgtagtg atcataccca
     9841 ttaatatgta agaatccatg aacagctaaa aatcccattt cacgctcaaa agagtgccca
     9901 tattcttggg actgctcacg cgccttatca atagaaataa acaattcgcc aatataagca
     9961 tcaaactctg ccatcatctc tgccaaacta ggatcagcag ccaaatcctc ttgactaaat
    10021 aaaataggcg tttctggctt atattctaga gaaatcacat ctgttggacg atcagtatct
    10081 cggtattcta agttgagttc atgacttctt tcattggtca caaaggtcac agacatttct
    10141 ttttcttctt tgcccgtctt ttgagcagca aaattgagca aatcaagtgt ttgctccata
    10201 atctcttgcg aaacttgtcc cgtttcgtca atcatctcga tatacataag gttaattgtt
    10261 agggaagggc tgcccacctc atttggtatg ttgggcaccc attattatct gacagataat
    10321 ttcccttatc ctttcaatca gcatttgtta tttacccatt ataccacaag ccctttaaaa
    10381 atgggaatgc taggtaaatc agggcctatt tggttagaat tgcctccaca cgtttttgca
    10441 agataggtaa taaatcctgc tcaaaccagg ggttcttagc taaccacaat tggtttcttg
    10501 gagaaggatg aacaagtgga aaatagtctg gcaagtaatc ctcaaagtga cggactgttt
    10561 cagttaaggt tttgtaagcc ttgttaccaa ggtaaaagtc ctgagcatag cgaccaacca
    10621 aaatcgtcaa ttcaacttct ggcatcaatg ctcttaatgg cgggtgccac ttagccgcaa
    10681 agccttctct aggtggtaag tcaccggatt ttcccttacc aggataatag aaatccatag
    10741 ggataatgcc aaacaatcct gagtggtaaa aggtgtcatt gtccacacct agccagtctc
    10801 gtaagcggat gccactacga tcattccagt aaagtttggt ttcttgagct actattccag
    10861 gagcttggcc aacaataata atcctggctg tttgaggagc atcatataaa ggaaaaatac
    10921 ctctttcggt ataggagaga ttagcctcat ctgccataat agctttggtt aaatcatcca
    10981 tagaatcctc cttaaaaagg ccatattaaa catcatcgta aaggtcttag tgctcttgac
    11041 cgatcacagg gtattttgtc aagcctggct ggtaatcagc ttcaacgtca ctagccttag
    11101 gttttagtaa gtccttcatc tcctctgagg acgtttcata agccttgata atatcagcca
    11161 caacaggatg tcggacaaca tctttagcag agaaatagac aaaatcaatc tgtttaatgc
    11221 cttgtaattt ctgagtagca tcaatcaaac cagacttgac atttcgaggg aggtcaatct
    11281 gactcgtatc tccattgaca atcattttag aattaaaacc aagacgggtc aaaaacatct
    11341 tcatttgcat gatggtcgtg ttttgagcct catctaagat aacaaaagcg tcatccagag
    11401 tacgaccccg catataagct aacggagcaa tctcaatcac atctcgttcc atcaggcgtg
    11461 tggtttgttc cttgcctaaa atgtgataaa gggcatcgta aacaggtctg aggtaaggat
    11521 ccaccttttc tttcaaatcg cctggtaaaa aacctaggct ttcgccagct tctaccgcag
    11581 gacgcgtcaa aataatacgc tttacctgtc ctcttttcag agcagtgact gcaagcgtaa
    11641 cagctaagaa ggttttccct gtccctgcag gtcctacacc aaaaaccaca tcatgacgtt
    11701 tgacactatc aacataggtt ttttgaccta atgttttgac tcgtattgcc ttaccatagt
    11761 tatctttaat gatttcttct tcataaagcg ccataaattg gtcaatctga tgagattctg
    11821 ccatagataa agctgtcacc acatctgatg tgttgaccac catgccacga cccactagaa
    11881 ccagtagggc t
//
