LOCUS       NZ_CP035434            15602 bp    DNA     circular CON 24-JUL-2024
DEFINITION  Streptococcus pyogenes strain emm75.1 chromosome, complete genome.
ACCESSION   NZ_CP035434
VERSION     NZ_CP035434.1
KEYWORDS    RefSeq.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1887700)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Atlas of group A streptococcal vaccine candidates compiled using
            large scale comparative genomics
  JOURNAL   Nat. Genet. (2019) In press
REFERENCE   2  (bases 1 to 1887700)
  AUTHORS   Davies,M.R., McIntyre,L., Mutreja,A., Lacey,J.A., Lees,J.A.,
            Towers,R.J., Duchene,S., Smeesters,P.R., Frost,H.R., Price,D.J.,
            Holden,M.T.G., David,S., Giffard,P.M., Worthing,K.A., Seale,A.C.,
            Berkley,J.A., Harris,S.R., Rivera-Hernandez,T., Berking,O.,
            Cork,A.J., Torres,R.S.L.A., Lithgow,T., Strugnell,R.A., Bergmann,R.,
            Nitsche-Schmitz,P., Chhatwal,G.S., Bentley,S.D., Fraser,J.D.,
            Moreland,N.J., Carapetis,J.R., Steer,A.C., Parkhill,J., Saul,A.,
            Williamson,D.A., Currie,B.J., Tong,S.Y., Dougan,G. and Walker,M.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005163865.1-RS_2024_07_24
            Annotation Date                   :: 07/24/2024 01:48:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,868
            CDSs (total)                      :: 1,779
            Genes (coding)                    :: 1,714
            CDSs (with protein)               :: 1,714
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 36 of 65
            Pseudo Genes (incomplete)         :: 31 of 65
            Pseudo Genes (internal stop)      :: 25 of 65
            Pseudo Genes (multiple problems)  :: 24 of 65
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:28:47
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 935572
            Orig. end                         :: 951174
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035434.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..15602
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..15602
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..15602
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:10602]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..10602
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    691..706
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     gene            complement(720..2231)
                     /locus_tag="ETT65_RS04875"
                     /old_locus_tag="ETT65_04875"
     CDS             complement(720..2231)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368530.1"
                     /locus_tag="ETT65_RS04875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04875"
                     /product="site-specific integrase"
                     /protein_id="WP_136075808.1"
                     /transl_table=11
                     /translation="MYYRTRQTKKGTIRYEIGEKYRDPLTGKWKNVTVSYTKNTSRARK
                     QAERDLQDKIAEAIALTENRYMPEKIHTFGQLKQNWLESWSVSVKSQTVKREELVIKRL
                     GQIIGDDYLLSKITPLLIKNSLSTYMSKYNASPSTMTHIKSTCNKIFNHGVLYNIIAYS
                     PMTAIKLDIPLEKKREVKRRREAKFLEIHELHAFFDTLRRRRNPSYYDLAIVLLFSGIR
                     IGEAAFTEEDFNMETGVLTIDKSLQYHDLRVEDYYFDSTKTINSERQVVLPKVACEATL
                     RAIERSKEFDCDAEANPCPSFKTSPSVFKTEYGSPITSRSFREVLARVEKELVETCEER
                     YGFKWKKHITPHSFRHMHITYLQSGDSGVTIREVMERVGHANFETTMGYTHKLPSAQEQ
                     ALETLNKFAQENGFEFRPVIKWSCKYSPLLQEIVDAHLSEGEIELGIDEFREIIGLAPT
                     YAPRHIASNILPKVRQDLSNIYHSFDIIGIKNKHNKMQSYRLVWD"
     gene            complement(2315..2551)
                     /locus_tag="ETT65_RS04880"
                     /old_locus_tag="ETT65_04880"
     CDS             complement(2315..2551)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693750.1"
                     /locus_tag="ETT65_RS04880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04880"
                     /product="DNA-binding protein"
                     /protein_id="WP_136075807.1"
                     /transl_table=11
                     /translation="MKIKYVSVENTLAEWGDKNSLLKRWEGLNKYTLNRWIAEMREHRE
                     FKQFIINPTHKLVFINLDGFEEFLRWKQRIAVK"
     misc_feature    complement(2821..2830)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 16.31"
                     /tool="antismash"
     gene            complement(2867..3262)
                     /locus_tag="ETT65_RS04885"
                     /old_locus_tag="ETT65_04885"
     CDS             complement(2867..3262)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /locus_tag="ETT65_RS04885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04885"
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_136075806.1"
                     /transl_table=11
                     /translation="MAYFIYVNGLKVPVSKEIYREYWRLTNRENYLQRLDIQYHVRPFS
                     DYTDALLTNVVVDDKMDLEKIIETREILQLLYEALLTLNDEEFTLVKDLFFEEKTLNEI
                     SQSNQISISTVARRRDKILNYLKKLLK"
     gene            complement(3628..4251)
                     /locus_tag="ETT65_RS04890"
                     /old_locus_tag="ETT65_04890"
     CDS             complement(3628..4251)
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379694.1"
                     /locus_tag="ETT65_RS04890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04890"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_136075805.1"
                     /sec_met_domain="Abi (E-value: 7.9e-16, bitscore: 55.0,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLIATQETTPTYVQWLASLGYLLIASIVLILVWKKYKNKDEKGK
                     FPFTWKDFGIAFLFYFATRLVAIGGTFLIQMVTGNATSANDAALMATNEQLLNMFPLYF
                     TAFQVAIGIFAPIMEELVFRGFFSHYFFKSNQKWLKLIISSTIFAVLHIVYPVEFITYF
                     TLGSIFYLAYARRGNIMDSIAVHLLNNSLLVIFSIVNYLLLILG"
     gene            complement(4328..4987)
                     /locus_tag="ETT65_RS04895"
                     /old_locus_tag="ETT65_04895"
     CDS             complement(4328..4987)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="ETT65_RS04895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="ETT65_04895"
                     /product="hypothetical protein"
                     /protein_id="WP_136075804.1"
                     /transl_table=11
                     /translation="MNFFYNIEETKLSTVCGGNPAGAAVVGGGVNAQQLYDYLYLGKSP
                     EQPAEELGNGEQNTTDQDEGAQPGVDLDNQANTEEISNQELSETVSSSDKTKADSSEQS
                     KEVTDKSIENVSTKDESLKIDVKEQENKEETLTKPVSSTSQVTPLASRTTEISGVSTLS
                     RKEETVSQQTPQTVSSISTLPRTGEVNSINLVRIGLALLLASGFTGISRLRIKQEE"
     gene            complement(5001..5254)
                     /locus_tag="ETT65_RS04900"
                     /old_locus_tag="ETT65_04900"
                     /pseudo=""
     CDS             complement(5001..5254)
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379882.1"
                     /locus_tag="ETT65_RS04900"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04900"
                     /product="Blp family class II bacteriocin"
                     /pseudo=""
                     /sec_met_domain="Bacteriocin_IIc (E-value: 5.5e-10,
                     bitscore: 36.2, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKTMEQFKVLNTDSLATVEGGKNNWQTNVWEGAHLLFLVGA"
     gene            complement(5841..7007)
                     /locus_tag="ETT65_RS04905"
                     /old_locus_tag="ETT65_04905"
     CDS             complement(5841..7007)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /locus_tag="ETT65_RS04905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04905"
                     /product="hypothetical protein"
                     /protein_id="WP_136075803.1"
                     /transl_table=11
                     /translation="MRTILSFELTKLLRKKSALGAVTVALLALLGLFYMHFFWSHISNG
                     YHGATVKGKAAVAINRQIAEEHSGYLSDDLISSILNDYAKNQPDLKKKGVYSVVSHYAI
                     NTLVPEPTEMLVAINQSDEVLQFDNIKLKSQEELGSHFPLGDLKLGNFAPWNQLFLVSN
                     SAYILVLLLSVYLSAPLFSGDFSKKMNPLLLTTKYGRNKLTVAKLTASLWISTSLFVVF
                     NAIILAVFAWYFGFSGWDTSVQLNLYWISPFENIMAFPEKLTLLSTFVNLVIFQFIGLI
                     FITAVNAFISSKTKSPLTTFAVSAGAIFVPSFLLQVFQGGLIHKLLTIISVSTSNTADL
                     MLQLSHKGPQGFFLDSFWLNGGLIIILRLLLVTFLIISTYKIIVRVQK"
     gene            complement(7004..7867)
                     /locus_tag="ETT65_RS04910"
                     /old_locus_tag="ETT65_04910"
     CDS             complement(7004..7867)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677582.1"
                     /locus_tag="ETT65_RS04910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04910"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_136075802.1"
                     /transl_table=11
                     /translation="MIVLLSKISKSYGSKSVLHQISAQLTPGLYGLLGANGTGKSTLLN
                     LLCHFTKPDSGTIELDGKREASYFYEQVGFLPQHFSYYGNFTGLDFLLYIATLKGMSKK
                     QAKKEADHLLRLVGLYDVRRKRIAAYSGGMKQRLGIAQALLNDPKILILDEPTVGLDPK
                     ERVKFRNIISDLSAHKIIILSTHIVSDVEAIAKEIIILKNGQFLQQGSSQDLLATMIGK
                     VWEFPVPDGSHLPSGDHFAVVNEKVTSSGRVFRVVSDDRPNQLAQLAQPTLEDLYIYHF
                     REEQAS"
     gene            complement(7950..8147)
                     /locus_tag="ETT65_RS04915"
                     /old_locus_tag="ETT65_04915"
     CDS             complement(7950..8147)
                     /GO_function="GO:0005186 - pheromone activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679509.1"
                     /locus_tag="ETT65_RS04915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04915"
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="WP_136075801.1"
                     /transl_table=11
                     /translation="MNTKTMEQFNTLSADKLAIVEGGFGYTWQCSDGKVSARHWFRKTA
                     EDNMKAYEKISGATCAIFHA"
     gene            8449..10602
                     /locus_tag="ETT65_RS04920"
                     /old_locus_tag="ETT65_04920"
     CDS             8449..10602
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /locus_tag="ETT65_RS04920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04920"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_136075800.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.5e-43, bitscore:
                     142.7, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1134.2,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAYKRTFVPQIDARDCGVAALASIAKFYGSNYSLAHLRELAKTN
                     KEGTTALGIVKAAKEMGFETRAIQADMSLFDMDDVPYPFIVHVTKEGKLQHYYVVYHNK
                     KDHLIIGDPDPTVKVTKMTKERFASEWTSVAIFLAPEPSYKPHKDKKNGLMSFLPLIFR
                     QRSLIFYIVLASLLVTLINIGGSYYLQGILDEYIPNQMKSTLGIISIGLIITYILQQMM
                     SFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEVISRFTDANSIIDALAS
                     TILSLFLDVSILTIVGSVLLVQNTNLFLLSLVSVPIYIIIIFAFMKPFEKMNNDVMQSN
                     SMVSSAIIEDISGIETIKSLTSEESRYQKIDSEFVDYLDKSFKLSKYSILQTTLKQGAQ
                     LILNIVILWFGAQLVMNGHISVGQLITFNTLLSYFTNPLENIINLQTKLQSAKVANNRL
                     NEVYLVESEFRNSQTLTDSQFLTGDIRFEDLSYKYGFGRDTLSDINLTIKQGDKVSLVG
                     ISGSGKTTLAKMIVNFFEPYKGRITINNNDLKLVDKKTLRQHINYLPQQAYIFSGSILE
                     NLTLGANQMISQEDIIRACEIAEIRQDIEQMPMGYQTELSDGAGLSGGQKQRIALARAL
                     LTKAPVLILDEATSGLDVLTEKKVIDNLMAMTDKTIIFVAHRLSISERTNQVIVLDQGK
                     VIETGSHHELMTKQGFYHHLFSK"
     misc_feature    complement(8460..8473)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 22.31"
                     /tool="antismash"
     misc_feature    complement(8464..8477)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 21.42"
                     /tool="antismash"
     gene            10614..11975
                     /locus_tag="ETT65_RS04925"
                     /old_locus_tag="ETT65_04925"
     CDS             10614..11975
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /locus_tag="ETT65_RS04925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04925"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_136075799.1"
                     /transl_table=11
                     /translation="MNPNLFKSAEFYQRRYHNFATLLIVPLILLVGFLLTFSLFAKKEV
                     TVTSRGEITPTTIIASIQSTSNNPITINNLANNQLVKKDELIIQYAETMESSQKQALET
                     QLATLNRQKTGLETLKASLSQGTNLFTEEDEFGYINTFNNFITQSQDIELGISKINSEV
                     SNQAALAGNTIAAIDTQINQLYHQISEYEELRQAITNKSLSLSSGNPHQDTLNNFLAQS
                     QQDSQTGLTDQYLSQINQSISGLEASIASLNIQRAGTGSVATYDNSFGTKVEVLRTQFL
                     QTTSQQLNTLETQITELTAQLEQASVRLENNTITAPETGVIHLNSEFEGKSLIPSGSEI
                     AQIYPALHHTQEALITYYVTSEHVALLKEKQEARLSLAKVGNQNITVIGKIQSIDKTAT
                     KTEDGNLFKVTALAQLSDKNSDVIQYGLQGRVTSVIAKKTYFDYYKDKILSNHD"
     gene            12073..12228
                     /locus_tag="ETT65_RS04930"
                     /old_locus_tag="ETT65_04930"
     CDS             12073..12228
                     /GO_function="GO:0005186 - pheromone activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515807.1"
                     /locus_tag="ETT65_RS04930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04930"
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="WP_136075798.1"
                     /transl_table=11
                     /translation="MTKQTKLLTHFSELTSSELHRISGGDWWSDWMKLFPRPKQTIDSN
                     NKHKLG"
     gene            complement(12277..13620)
                     /locus_tag="ETT65_RS04935"
                     /old_locus_tag="ETT65_04935"
     CDS             complement(12277..13620)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164876.1"
                     /locus_tag="ETT65_RS04935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04935"
                     /product="GHKL domain-containing protein"
                     /protein_id="WP_136075797.1"
                     /transl_table=11
                     /translation="MSIIEYTIEAIVSLGSLIVLFFSVNQLKYAARSIFLVILLRVSVS
                     ALFATLNQLFSNQFFSYFDEPLYGLLLAVVFLRPLPKTLLIFYGLLPFTLENLFYRMVT
                     YFILPLLGQPPGVIEDTPSFVLTILLSLLAVLFFLKWLKYDFVKLRTDNIGTEDKRLLN
                     LTNWVMVVYYLLIQTLTYLEYDIGLSTMAYRQFILVTYLVIFMGIIKQLDVHLRQKLRD
                     QLDFQQDLQVKSLEDYSNQVEDLYREVKGFRHDYSNLLTTLRLAIEENDMLQIKEIYQS
                     VLKDSHKQLRASKYDIGRLVNIDNSALKSLLASKLLQANDSHVSVSLEVPEVIKPQGME
                     LVDFITIVSILCDNAIDAAINAASPKLTIAFLLVNDKQMFIIENNTKEERVDLSKLYSF
                     GYSSKGDNRGIGLYNVMKILERYPHVSLNTTSASYLFGQSLEIDLSSK"
     gene            complement(13634..14371)
                     /locus_tag="ETT65_RS04940"
                     /old_locus_tag="ETT65_04940"
     CDS             complement(13634..14371)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /locus_tag="ETT65_RS04940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04940"
                     /product="response regulator transcription factor"
                     /protein_id="WP_136075796.1"
                     /transl_table=11
                     /translation="MNIFVLEDDFKQQARIEKTIVNLLEKHRIVPKKFEISGKPDQLLQ
                     YIEEKGAHQLFFLDIEIKEEELKGLQVAKQIRELDPYASVVFVTTHSEFMPLSFRYQVS
                     ALDYIDKEFETAEFESRIEKALLYVHAKDHKAVADDSFYYKSKYAHVQYPFNEVYYIET
                     SPRPHRVILYTKTDRMEFTASLSDILKQDKRLLQCHRSFAINPRNVAKVDKHEKQVYFP
                     NGSSCLISRKKLESILAAIDKLH"
     gene            complement(14373..14714)
                     /locus_tag="ETT65_RS04945"
                     /old_locus_tag="ETT65_04945"
     CDS             complement(14373..14714)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841624.1"
                     /locus_tag="ETT65_RS04945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ETT65_04945"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_227877714.1"
                     /transl_table=11
                     /translation="MESIIVQTRQECLKLKIEDIYYIMSHPSRPHCIQIVTDDSNYNFF
                     QSLQIIEDQYSGYFVRCHRNCLVNLAKIKSINVKERVIVLGERGQYQVAFSRRRHQKIL
                     QEWINQGGV"
     gene            14913..15308
                     /locus_tag="ETT65_RS09680"
     CDS             14913..15308
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014638214.1"
                     /locus_tag="ETT65_RS09680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_227877846.1"
                     /transl_table=11
                     /translation="MIEIFPISWLSIVVIAIVKYYLILHNINKQIKLIFYYRPYKSHSP
                     TVYFIIKSRFLKSEKMYAYLEHIRSQSDLATIIIIGSHINYDELFKNHYRVFGVIDTTE
                     NKSLAFIRDQVHFYLDALYGSNQNQAS"
ORIGIN
        1 gccaaagata gtcacttcat ccattggtag taattggtcc ttgatagcac ctgttaaagc
       61 taattttaag cccaccaatt cgtcctcaaa ttttggccat aaaataccag gagtgtctag
      121 aatttccaat tccttattgg acttcaacca ttgttgtcct ttagtcacac caggtttatt
      181 gccaactaca gcaattttct taccagccaa gcgattcatc aaggtagatt taccagcatt
      241 tggaatacca ataatcatgg ttcgcaaggt ttctttttga atgccgcgct ctcgcaagcg
      301 ttgaatttta tcagccatga gttccttggc tgcttctgtt acttttttaa ccgttgattg
      361 ttctttggaa ttgatggcta atgttttaat accttgactt tcataataag ccttccactc
      421 tttcgtacga gtggcatcgg ccaagtcagc tttatttaaa atcattaatt taggtttatc
      481 accaacaatt tttgtaagca tgggattttg actagaaagt ggtaagcgtg catccactaa
      541 aatagttaca aagtcaacat gctttacatt ttcctgaacc tgtcttctgg ctttggacat
      601 gtgcccagga aaccattgaa tagtagccat gttaaaaaaa tcctttcgta tcaagggttt
      661 tcagccctta gtttttaaat aaacatgcga gtcaccaaaa agtcaccaag tgttggacac
      721 taatcccaga ccaaacggta tgattgcatt ttattatgtt tatttttaat tccgataata
      781 tcaaatgaat ggtatatatt tgatagatct tgtcggactt ttggcaaaat atttgatgca
      841 atatgccttg gagcataggt cggtgcaaga cctataattt ctctgaactc atctattcca
      901 agctctattt ctccttcaga gagatgcgca tcaactattt cttgtaataa tggagaatat
      961 ttacaagacc actttattac tggtctaaat tcaaacccat tttcttgagc aaacttattt
     1021 agagtttcca gagcttgttc ttgagcagat ggtagtttat gggtataacc cattgtagtt
     1081 tcaaaattag catgtcctac tcgctccatg acttctctaa tagttacacc tgaatcacct
     1141 gactgcaggt aagtaatatg catatgtcta aaagaatgtg gtgtaatatg ttttttccac
     1201 ttgaaaccat atctttcttc acaggtttct actaattctt tttcaactct cgctaaaact
     1261 tctctaaatg agcgcgaagt aatcggtgag ccatattctg ttttaaaaac acttggtgat
     1321 gttttaaaac ttgggcaggg attagcttca gcatcgcaat caaattcttt actgcgttct
     1381 atcgctctca aagtagcttc acatgctact ttaggtagaa caacttgtcg ttcagagtta
     1441 atggtcttgg tactatcaaa gtaataatct tcaactcgca aatcatgata ttgaagtgat
     1501 ttatcaattg tcaacacacc agtttccata ttaaaatctt cctctgtgaa cgcagcttct
     1561 ccaatccgta ttccagaaaa taaaagcaca atagctaaat cgtagtagct tgggttacga
     1621 cgtcttctca acgtatcaaa gaaagcatgt agttcatgga tttccaaaaa ttttgcttct
     1681 cgtcgtcttt ttacctctcg ttttttctca agtggtatat ccaacttaat cgccgtcata
     1741 ggtgaataag cgatgatatt atatagaacg ccgtgattaa agatcttatt gcaagtgctt
     1801 tttatatgtg tcatggttga cggtgaagca ttgtacttag acatataggt tgaaaggcta
     1861 ttttttatca acaatggtgt aatcttactc agtaagtaat catctccaat aatctgacct
     1921 aaccgcttga tgacaagttc ttcacgcttc actgtctgag atttaacaga cacagaccat
     1981 gattctagcc aattttgctt taattgaccg aatgtatgga ttttttcagg catgtatctg
     2041 ttttctgtta aagcgatggc ttcagctatc ttatcttgaa ggtcacgctc tgcttgtttt
     2101 ctcgctcgac tagtattttt tgtgtaagaa acagtaacgt ttttccactt accagtcaaa
     2161 ggatctctgt atttctcacc tatttcatag cgaatagttc ccttcttagt ttgtcttgtt
     2221 ctatagtaca tacttccatc ctctcaaata aaaaagaaaa acgaaatagt tctagtagaa
     2281 taaatagcca tctactatta tagcacgatt tcctttattt gaccgctatt cgttgcttcc
     2341 atcgtaaaaa ctcctcaaag ccatcaagat ttataaaaac taatttatga gtgggattta
     2401 tgatgaattg cttaaattct ctatgctccc tcatttcagc aatccaacga tttaaggtgt
     2461 acttgtttaa tccctcccag cgttttaaga ggctattttt atccccccac tcagctaatg
     2521 tgttttcaac tgaaacgtat tttattttca tacattctcc ttttacttat attcatgacc
     2581 tccgtgggta gaactggtta ggttctacat ggggattgca cccactgtcc tttctcgatg
     2641 gcagcctaaa cggttagaag tatcattatc ctcatttgtc tcatggcaga taggtgacca
     2701 acctatttta tagtcaacat tattcgctca tatccttgcg tgtctaacta aattgcttta
     2761 caaatgatta atgtcccacc ttggtctatt cagttgtcaa tgggcattta aggaaccata
     2821 gtcccccttc actatataga gtgaaaaaat gataactttt accgccttac ttcaagagct
     2881 ttttgagata gttcaaaatt ttatccctcc gtcttgcaac agttgaaata gaaatttgat
     2941 tagactgaga aatttcattc aatgttttct cctcaaaaaa caagtccttg accagggtaa
     3001 actcctcatc attaagcgtt agtagggctt cgtatagtag ctgcaaaatt tccctagtct
     3061 caataatttt ttcaagatcc attttgtcgt caaccacaac gtttgttaat agagcgtctg
     3121 tataatcaga aaacggtcta acatgatact gaatgtctag cctttgtagg taattctctc
     3181 gattggttaa tcgccaatat tcgcgatata tttctttgga aacagggact tttaacccgt
     3241 tcacatagat aaaatatgcc atcttaatcc tccttattta atcttgataa ggctgaaaaa
     3301 ggcggtgaac gatttttcat aaaacactct caatttttag acttcactat taagagtgaa
     3361 aattttcatg gtttttaccg ctgttttcac aaaaaaatat aaaaaagcga taaagcaaac
     3421 tgctctatcg ccaaatatta tcactacatg agttcagtta gtggttttac tggtctcggt
     3481 tttggaattg gttttggata ctaaaaattt ggaaatttgt atccaatacc ataaattggt
     3541 gtcgaaaaat ctccacctgt aatggcggaa agagcaacag caactaattg tttgatcatc
     3601 atgtgatgat acctcctttt aaaattttta acctaagatt aacaataaat aattaacgat
     3661 agagaaaatc actaacaagc tattattcaa caaatgcaca gcaattgagt ccatgatatt
     3721 tccacgcctt gcgtaagcca agtagaaaat tgaccctagt gtaaaatatg tgatgaattc
     3781 taccggatat acaatatgca agactgcaaa aattgtagaa ctgataatca gtttcaacca
     3841 tttttgatta ctcttgaaaa aataatggct aaagaatcct cggaagacta attcttccat
     3901 aatcggtgcg aaaataccaa tagcgacttg aaaagctgta aaatagagag gaaacatatt
     3961 taacaactgc tcattggttg ccatcaaggc tgcatcatta gctgatgttg cattacccgt
     4021 gaccatttga attaaaaagg tcccaccaat ggctaccaga cgagtagcaa aataaaagag
     4081 aaaagcaatt ccaaagtctt tccaggtaaa ggggaatttt cctttctcat ccttgttttt
     4141 atattttttc caaacaagaa taaggacgat actagcaatc aataaataac ctagactagc
     4201 aagccactgc acatatgttg gagttgtctc ctgagttgca atcaatctca ttggaaagat
     4261 attgacactt aaggtaagaa agattagtcc aataaatttc aataaagttt taagacgtgc
     4321 aatgttttta ctcctcctgt ttaattctca aacgactgat gcctgtgaat cccgaagcaa
     4381 gcaagagggc taaaccgata cgtacaagat taatcgaatt gacctctcct gttctaggca
     4441 aggttgaaat gcttgaaaca gtctgagggg tctgttgact tactgtttcc tcttttctag
     4501 acaatgttga gacgcctgag atttcagtag tacgacttgc taatggtgtg acctgtgatg
     4561 tggagcttac tggcttagtc aaggtttctt ctttattttc ttgctcttta acatcaattt
     4621 tgagactctc atctttggta ctgacatttt cgatagactt atctgtaacc tctttggatt
     4681 gttctgaaga atcagccttg gttttatcag aggaagagac ggtttcggac aactcttgat
     4741 tcgaaatctc ctcagtgtta gcttgattat ctaagtcaac gcctggttgt gccccttcgt
     4801 cttgatcggt tgtattctgt tcaccattcc caagctcttc tgcaggttgt tcaggtgatt
     4861 ttccaagata gaggtagtcg taaagttgct gagcattaac tcctccaccg acaacagctg
     4921 ctcccgcagg attcccgcca caaacggtac tcaacttagt ttcttctata ttataaaaaa
     4981 aattcatctg tccctctcta ttaaaaacca cctgttgctc ctgtaactcc tgcccataga
     5041 gctacaccaa atttagctcc aatgtaacca caagctccca taaacggtgt accaacacca
     5101 cttgctgcac aaatagctgt tcctaagccc caaccagaaa tagcagatga gccccttccc
     5161 aaacattagt ctgccaatta ttttttcctc cctctacagt agccagtgaa tctgtattga
     5221 gcactttaaa ctgttccatt gttttggtat tcatgatgaa tacctccttt atgatataga
     5281 tttatatttt gtcgggttga acctcgacaa gtctagcata gcacttcctc aaaaatatga
     5341 ctgcgttttt cttctctata cgaattaact ttctcgcagc cttaattgtg attaaatttt
     5401 ttctactcga taaatattac catctaaagc attctaaata ctttgacatt cattactgtc
     5461 aatacttagt tcatacacgt cctatcatat ttttaaaaaa cttgatagga tgcgttttta
     5521 ctagaaaaga atagcttatt tggttgatac attatcaaaa gtgatactag acccaaagct
     5581 agcaaacaga attgaactaa acaataatga tttgagtaac ctattcttca taatgaagct
     5641 cctttatgtg tgatagcttc attttacact ttatcctaca aaaaactttc ctatttcctg
     5701 aacagtcatc gaaacgtctc gaatggcaaa tatttactat ttcaatcagt cactatacat
     5761 atcaattatc tctaaaaaat aaaacctctt gatttagctg ttgcaatcaa aaggttcaat
     5821 ttatattatt tattctcaat ttatttttgc actctcacaa ttattttgta tgtagaaatt
     5881 atcaagaaag taactagtaa tagtctgagg ataattatca aaccaccatt caaccaaaaa
     5941 ctatccaaga aaaatccctg aggcccttta tgagacagct gtaacatcaa atctgctgtg
     6001 tttgacgttg acacagagat aattgtcaac aatttatgaa taagcccgcc ttgaaagact
     6061 tgtaggagaa aacttggcac aaaaatcgca ccagcagaca cggcaaaagt agttaacggg
     6121 gacttggttt tgctggagat aaaggcattg acagctgtga tgaagataag accgataaac
     6181 tgaaaaataa ctaggttgac aaaggttgac agaagtgtca atttctctgg aaaagccatg
     6241 atgttttcaa aaggactaat ccagtaaagg ttaagctgga cactagtatc ccagccgctg
     6301 aaaccaaaat accaagcaaa gacggctaaa atgatggcgt tgaaaacaac aaataaactg
     6361 gtgctaatcc ataaactagc tgtcaactta gccactgtca acttattacg accgtacttg
     6421 gtcgttaata agagaggatt catcttttta gaaaaatcac cagaaaagag aggggcagat
     6481 aggtagacag acaaaagcaa gacaaggata taggcgctgt tactgactag aaagagttgg
     6541 ttccaagggg cgaaatttcc cagcttcaag tccccaagtg ggaaatgcga gcctagctct
     6601 tcctgagatt tcagtttgat gttatcaaat tggagcacct catcactctg gttgatggca
     6661 accagcatct ccgttggttc tggaactagg gtattgatgg cgtaatgaga aaccacgctg
     6721 taaacccctt ttttcttgag gtcaggttga tttttggcgt aatcattgag gatggagctg
     6781 attagatcgt cagataaata gccactatgc tcctcggcaa tctgccgatt gatagcaacg
     6841 gctgccttac ctttaacagt agcaccgtgg taaccattac taatatgact ccagaagaaa
     6901 tgcatataaa acaagccaag caaggctaat agggcaaccg ttactgctcc tagagctgat
     6961 tttttacgga gtaatttggt caattcaaag gataagatag ttctcatgag gcttgttcct
     7021 ctctaaagtg gtagatatag aggtcttcca aggtcggttg agcaagttgt gctagctgat
     7081 ttggtcgatc atcgctgacc acacggaaca ctcggccaga tgaagtcact ttctcattga
     7141 ccacagcaaa gtggtcaccg cttggcagat gtgaaccgtc aggcacagga aactcccaga
     7201 ccttaccaat catggtcgca agcaagtctt ggctactgcc ctgctggaga aattgcccat
     7261 ttttcaaaat gataatctcc ttggcgatag cctccacgtc ggaaacgatg tgggtggata
     7321 ggataatgat tttgtgggca gacaggtcac tgatgatgtt gcggaattta acccgctcct
     7381 tgggatccag ccccaccgtc ggctcgtcca aaatcagaat tttcgggtca ttgagtaggg
     7441 cctgagcgat gcctagccgc tgcttcatgc cacccgagta ggcagcaatc cgcttgcgac
     7501 ggacatcgta aagccccacc agtcgcagca agtggtcagc ctcttttttg gcctgttttt
     7561 tagacatacc ttttaaggtt gctatgtaga gtagaaagtc cagacctgta aagttgccat
     7621 aatagctaaa gtgctggggc agaaacccta cctgctcata aaagtagcta gcctcacgct
     7681 taccgtccag ctcgatggtt ccgctgtcgg gtttggtaaa atggcagagc aggttaagca
     7741 gggttgactt gcccgtccca tttgccccca gcaaaccata gaggcctggg gtcagctgag
     7801 cagaaatttg atggagaaca ctctttgagc cataactctt agaaatctta ctgagtaata
     7861 ctatcataat ctatattcct taatcaataa aatctcccta ttcagcaatc tataaagcta
     7921 aatagggaga atacaagact atctacacat tatgcgtgaa aaatagcaca tgtcgcacct
     7981 gagatttttt cataggcttt catgttatct tccgctgtct ttcggaacca atgacgcgcg
     8041 ctaactttac catctgaaca ttgccacgta tacccgaatc ctccctcgac aatcgcaagc
     8101 ttatcagcgc taagtgtgtt aaactgttcc attgtttttg tattcatgat gaatacctcc
     8161 tttatgatat agattttata ttttgtcggg tcgcaacctt gacaagtcta gtatagcact
     8221 tcttcaaaaa atagttctcc catttcctga acagtcatcg aaacgtccta aacagtaaca
     8281 ttttaccgtt taagacgata atagaaccgt tcaggagttc tttttaactc caagattttt
     8341 ctgtgttttg ccattcagga cgtttcgatg accatttaag atttggacgt ttttttcttt
     8401 aattttttgt cataatagtc ttgtcactaa caagaaagga tttgataaat gacagcttac
     8461 aaacgaacct ttgttcctca aatcgatgcc agagactgtg gtgtggctgc tttagcctct
     8521 attgctaagt tctatggctc caattactct ttagcccatt tgagggaatt agctaaaacg
     8581 aataaagaag gaacgaccgc tcttggtatc gtcaaggcag ccaaagaaat gggctttgaa
     8641 accagagcca tacaggcgga tatgagtctc tttgatatgg atgacgttcc ctatcccttc
     8701 atcgttcacg tcactaaaga aggcaaactg caacactact atgtggtcta tcataacaaa
     8761 aaagaccacc tcatcattgg tgatcctgac cctacggtca aagtcaccaa gatgaccaag
     8821 gagcggtttg cttctgaatg gacaagcgtc gccatctttc tggctccaga acccagctat
     8881 aagcctcaca aggataaaaa gaacggcctg atgagctttc tccccttgat tttcaggcaa
     8941 cgttctctta tcttttatat cgtcttggct agtcttctag tgacacttat caatattggt
     9001 ggttcttact acctgcaagg gattttggat gaatacatcc ctaatcagat gaagtcaacc
     9061 ttaggtatta tctccatcgg tctcatcatc acctacatcc tccagcagat gatgagtttc
     9121 tcacgggatt acctcttgac cgtgctcagc cagcggctaa gtattgatgt gattctctcc
     9181 tatatccgcc acatctttga actgcctatg tccttctttg cgacacggcg gactggcgag
     9241 gtcatctctc gctttacaga tgccaattcg atcattgatg ctctggcttc gaccattttg
     9301 tcactcttcc ttgatgtttc catcttgacc attgttggta gtgtactttt ggtacaaaac
     9361 accaaccttt tccttctctc tctggtctca gttcccattt atatcattat tatttttgct
     9421 tttatgaagc cgtttgagaa gatgaataac gatgttatgc agagcaattc catggtcagc
     9481 tctgccatta tcgaagacat cagcggcatt gaaaccatca aatccttgac cagcgaagaa
     9541 agccgctacc agaagattga tagcgaattc gtagattacc tggacaaatc cttcaaactc
     9601 agtaaatact ctatccttca aaccaccctt aaacaggggg ctcagctcat tctaaatatt
     9661 gtcatcttgt ggtttggggc tcagctagtc atgaacggac acatttctgt tggacaactg
     9721 attaccttca acacccttct atcttatttt accaatcctt tagaaaatat tatcaacctc
     9781 caaaccaagc tccagtctgc caaggtggct aacaatcgcc taaatgaagt ctacttagtt
     9841 gaatctgaat tcagaaacag tcagacctta accgattcac agttcctgac tggcgatatt
     9901 cgctttgaag acctttccta taagtacggt tttggacgtg acaccctatc tgatatcaat
     9961 ctgaccatca aacaaggcga taaggtcagt ctagtgggga ttagcggctc aggaaagaca
    10021 accttggcca agatgattgt caacttcttt gagccttaca agggccgaat caccatcaac
    10081 aacaacgatc ttaaactggt tgataaaaag acgttacgac agcacatcaa ctacctcccc
    10141 caacaagctt atatttttag tggctcgatc ttagaaaacc taactttggg agctaatcaa
    10201 atgattagcc aagaagatat tatcagggct tgtgagatcg ctgaaattcg ccaagatatc
    10261 gaacaaatgc ccatgggtta tcaaacagaa ttgtccgatg gggcaggctt atcaggtggg
    10321 caaaaacaac ggattgcttt ggctagggct ctccttacta aagcccctgt tcttattctt
    10381 gatgaagcca ccagtggtct tgatgtttta acggaaaaaa aggtcattga taatctgatg
    10441 gccatgacag ataagaccat tatctttgtc gcacaccgcc tcagtatttc tgaacgaacc
    10501 aatcaagtta tcgttcttga ccaagggaag gtcattgaga ctggttcaca tcacgaatta
    10561 atgaccaaac aaggtttcta ccatcatcta tttagcaagt aaggagacaa agcatgaacc
    10621 ctaatttgtt caaaagtgct gaattttacc agcggcgcta ccataatttt gcgacactct
    10681 taattgtccc cttgatatta ttagtcggtt ttcttctaac cttctccctt tttgccaaaa
    10741 aggaggttac cgtcacatca agaggtgaga ttactccgac gacaatcatc gcttccatcc
    10801 aatccaccag caataaccct atcacaatca ataacctagc caataaccag ctggttaaaa
    10861 aggatgagct catcatccag tacgctgaaa ctatggagag ttcccaaaaa caagcccttg
    10921 aaacccagct agccactcta aatagacaga aaactggact cgaaaccctc aaagcaagtc
    10981 tgtctcaggg aacaaatctc tttacagagg aagatgaatt tggctatatc aatactttta
    11041 acaactttat cactcaatcg caggacatcg aactagggat ttccaaaatc aattccgagg
    11101 tgagtaacca agctgcccta gcaggtaata ccattgctgc gattgacacg caaatcaatc
    11161 aactctacca ccagatttca gaatacgagg aacttcgcca agctattacc aataaaagtt
    11221 tgagtttgtc atctggaaat ccacaccaag acaccttaaa caatttctta gcccaatccc
    11281 agcaagactc ccagacagga ttaactgatc aatacctttc tcaaatcaac caaagtatct
    11341 cagggctaga agcatccata gctagtctga acatccaacg agctggaact ggttctgttg
    11401 cgacctatga taacagcttt ggaacaaagg ttgaagtgct aagaacacag tttttacaaa
    11461 cgacctctca gcagctgaac actcttgaaa cgcaaatcac agagctaaca gcacagctag
    11521 agcaagcctc cgtccgacta gaaaacaata ccattacagc tcctgaaaca ggggtaatcc
    11581 atctcaatag tgagtttgaa ggaaagtctt tgattccaag cggtagtgaa atagctcaaa
    11641 tttaccccgc tctccatcat acccaagaag ccctcattac ttactatgtg acctcagagc
    11701 atgttgccct actcaaagaa aaacaagaag cacggctgag cttggcaaag gttggtaatc
    11761 agaacatcac tgttatcgga aagattcagt ctatcgataa aaccgctact aagacagaag
    11821 atggaaacct cttcaaagtc actgcattag ctcaattatc cgacaagaat agtgacgtca
    11881 tccagtacgg tctccagggt cgagtcacga gtgtcattgc taagaagact tactttgatt
    11941 actataagga taaaatttta tccaaccatg attaaccatt taggaaagtt aaatgactgt
    12001 tcaggatttg aatgtcaaaa atctccatat tttgtgtaca atgaagtaaa aattatataa
    12061 ggaggtcctc acatgaccaa gcaaacaaaa ttactcacgc atttctcaga actaacctct
    12121 tcagaactcc atcgtatttc cggtggggat tggtggagcg attggatgaa actttttcct
    12181 aggccaaagc aaaccataga cagcaataat aaacataaac tgggctaaca tcaaaaaagt
    12241 tggaccgagg gattaaatcc catggtccaa ctttttttat ttcgatgaca aatcaatttc
    12301 tagggattgc ccaaacagat aagatgcact agtcgtgttt agtgatacgt gtggataccg
    12361 ctctagaatt ttcatgacat tatataaacc aatacctctg ttatcccctt tggaactata
    12421 accaaaggaa taaagttttg aaaggtcaac tctttcttcc ttggtgttat tttcgataat
    12481 aaacatctgc ttatcattga ccagcaaaaa agcgatggtt agctttggac tggctgcatt
    12541 tatcgcagcg tctatggcgt tatcacataa tatcgatacg atggtgataa aatctaccaa
    12601 ctccattcct tgtggcttaa tcacttctgg cacttccaaa ctaactgata catgactatc
    12661 attagcttgc aagagcttag acgccagtag actcttcaaa gcagagttat caatattgac
    12721 caagcgtcca atatcgtatt tactggctcg taactgttta tgtgaatcct ttaataccga
    12781 ctgataaatc tcctttattt gcaacatatc attctcttca atagccaagc gtagtgtcgt
    12841 tagtagatta gaataatcgt gcctaaaccc tttcacttca cgataaaggt cctcaacctg
    12901 attactataa tcttccaagc ttttgacttg caagtcctgt tgaaaatcaa gctggtctcg
    12961 tagtttctgc cgcaaatgaa cgtcaagttg tttgataatt cccatgaaga taacaaggta
    13021 agtcactaga ataaattgac gataagccat tgtcgacaat ccgatatcat actctaaata
    13081 agttaaggtt tgtattaaaa ggtagtatac aaccattacc cagttggtca gattgaggag
    13141 ccgtttatct tcagttccaa tattgtccgt tctcaacttg acaaaatcat acttcaacca
    13201 ttttaagaag aagagaacag cgagtaaaga cagcagaata gttaaaacaa aagaaggtgt
    13261 gtcttctata actcctggtg gctgacccaa taaaggtaaa ataaaatagg ttaccatgcg
    13321 ataaaagaga ttttctaggg taaagggaag caagccatag aaaatcaata gggttttagg
    13381 cagtggtctt aaaaacacaa cagctaataa cagaccatac aaaggttcat caaaataaga
    13441 aaaaaattga tttgaaaata actgatttaa ggtcgcgaaa agagctgaaa cggatactct
    13501 gagcagaata accaaaaaga ttgaacgggc agcatacttt aattggttaa cactaaaaaa
    13561 gagaacaata agtgaaccca atgaaacaat ggcttcaata gtgtattcaa taattgacat
    13621 actatctcct taatcaatgc aacttatcaa tagcagccaa tattgattcc agtttttttc
    13681 ttgagataag acaggaagaa ccattaggaa aatagacttg tttctcatgt ttatccactt
    13741 tagcaacatt tctcggattt attgcaaagg aacgatggca ttgcaaaagt cgtttatctt
    13801 gtttcaaaat atctgacaaa ctggctgtaa attccattcg gtctgttttg gtataaagga
    13861 taacacgatg aggccgtgga gaggtttcta tataataaac ttcattaaat gggtattgca
    13921 catgagcata tttagactta taatagaacg aatcatctgc tactgcttta tggtccttag
    13981 cgtgaacata tagtaaagcc ttttctattc gggattcaaa ttcagcagtt tcaaattctt
    14041 tatcaatata atctaaagca gatacttgat accggaaaga caacggcata aattctgaat
    14101 gtgtcgttac aaaaacaaca ctagcataag gatcaagctc cctaatttgt ttagcgactt
    14161 gaaggccctt aagttcctcc tctttaattt caatatccaa aaagaacagc tggtgtgcac
    14221 ctttttcttc tatatattga agcaactgat caggctttcc tgagatttca aatttttttg
    14281 gaactattct atgcttctct aacaaattaa caatagtttt ctcaattctc gcttgttgct
    14341 taaaatcatc ctctaataca aagatattca catcatactc ccccttgatt tatccactct
    14401 tgtaaaattt tctggtgcct acgccttgaa aaagcaactt gatactgccc tctttctcca
    14461 agaacaataa ctctctcttt aacattaatt gacttaattt tagcgagatt aacaagacag
    14521 tttcgatgac atctcacaaa gtaacctgaa tattggtctt caataatctg tagagactga
    14581 aaaaaattat aatttgagtc atccgttaca atttgaatac aatgaggtct gctaggatgt
    14641 gacattatat aataaatgtc ctctatcttg agttttagac attcttgcct agtttgtaca
    14701 ataatcgatt ccatggttat atccctccta tagacaatat accatgaaag caacatcata
    14761 ttcccttgaa atcctaaacg gttatccaaa cgtcttgaat ggtaagtttt ttactattta
    14821 ggacatttca tctacttttc aggaaacaac tctagttaac actttatatt tggtaaaata
    14881 taattcaata ataaaaatag gagtgagaca taatgattga aatttttcca atatcttggt
    14941 taagtattgt tgtgatagct atcgtcaaat attatctaat tcttcataat atcaataagc
    15001 aaatcaaact aattttctac tatcgaccat ataaatccca ctctcctact gtatatttta
    15061 tcataaaatc aaggttttta aaatcagaaa aaatgtacgc ttatttagag catattcgct
    15121 cgcagtcaga tttagcaacc attataatca ttgggagtca cattaactac gacgaactat
    15181 tcaaaaatca ttatcgggta tttggtgtta tcgatacaac tgaaaacaaa tcactagcct
    15241 ttattcgaga ccaagtccat ttttaccttg acgctcttta tggctccaat caaaatcaag
    15301 caagttaagg ttttttataa accgttctag tcttctaact ttatattcgt aaaaatcaat
    15361 agattcttca aggttctttt gaaacatttt cacctctagc tctacctgtt caagtgcaat
    15421 agctgcggtc aggttcatct tggcatagtc atatctggca agccgtctgt tttctgggtc
    15481 tgcgttatca agattgaata agacctctgc aactttatta agatagacaa gtttatcttg
    15541 ggaatctatg atggtcagct ccagttgagc aatgtcttga atcatctcat ccttaatctc
    15601 ct
//
